CN104206691A - Feed for penaeus vannamei and preparation method of feed for penaeus vannamei - Google Patents

Feed for penaeus vannamei and preparation method of feed for penaeus vannamei Download PDF

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CN104206691A
CN104206691A CN201410474642.5A CN201410474642A CN104206691A CN 104206691 A CN104206691 A CN 104206691A CN 201410474642 A CN201410474642 A CN 201410474642A CN 104206691 A CN104206691 A CN 104206691A
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feed
dregs
prawn
penaeus vannmei
content
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CN201410474642.5A
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Chinese (zh)
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程宗佳
张志勇
柏世军
姜思然
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中粮营养健康研究院有限公司
中粮集团有限公司
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Priority to CN201410474642.5A priority Critical patent/CN104206691A/en
Publication of CN104206691A publication Critical patent/CN104206691A/en

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Abstract

The invention relates to the field of animal science and discloses a feed for penaeus vannamei and a preparation method of the feed for penaeus vannamei. The feed for penaeus vannamei comprises alga beta-1, 3-glucan, wherein the content of alga beta-1, 3-glucan is not more than 0.10wt%. The feed for penaeus vannamei added with alga beta-1, 3-glucan can significantly improve the protein content of penaeus vannamei. Experiments show that the feed for penaeus vannamei can significantly reduce the water content of body components of penaeus vannamei, improve the protein content (p<0.05) and further significantly improve the protein content of penaeus vannamei.

Description

Feed of a kind of Penaeus Vannmei and preparation method thereof
Technical field
The present invention relates to animal science field, be specifically related to feed of a kind of Penaeus Vannmei albumen and preparation method thereof.
Background technology
Shrimp is the long body animal of one way of life in water, belongs to arthropod shell-fish, and kind is a lot, comprises freshwater shrimp, river prawn, grass shrimp, cray, prawn, prawn, base enclose shrimp, Chinese lute shrimp, lobster etc.Shrimp contains rich in protein, and nutritive value is very high, and its meat is the same with fish soft, easy to digest, but without fishy smell and spur, simultaneously containing abundant mineral matter (as calcium, phosphorus, iron etc.), has benifit to the healthy pole of the mankind.According to the analysis of science, shrimp edible part protein accounts for about 16 ~ 20%.Also containing abundant magnesium in shrimp, magnesium has important regulating action to cardiomotility, well can protect cardiovascular system; it can reduce blood cholesterol level; prevent artery sclerosis, simultaneously can also coronary artery dilator, be conducive to preventing hypertension and myocardial infarction.Different shrimps kind life habits is different, and the feed be applicable to is also different.Penaeus Vannmei (Litopenaeus vannamei Boone, 1931), formal name used at school Environment of Litopenaeus vannamei Low, belonging to Arthropoda Crustacea Decapoda swimming suborder Penaeidae Penaeus Lito-penaeus subgenus, is wide temperature eurysalinity torrid zone shrimps.Penaeus Vannmei is one of three large shrimp species that current world cultured output is the highest, has, fast growth low to water environment high-output stress-resistance, nutritional requirement, and shrimp body is containing plurality of advantages such as meat amount are large; In recent years, its intensive culture scale is increasing, and 2011 annual productions reach 660,000 tons (fishery yearbooks in 2012).
But in recent years, due under high-density breeding environment, often cause the stress reaction of shrimp body, its immune function sustains damage, and have impact on the survival of shrimp, growth and quality, causes Penaeus Vannmei disease to take place frequently, output declines greatly, and economy is subject to extreme loss.Therefore, the impact of feeding quality on the growth of Penaeus Vannmei and the raising of quality improving Penaeus Vannmei is most important.At present, feed is not also had can to improve growth performance and the quality of Penaeus Vannmei very well.
Summary of the invention
In view of this, feed that the object of this invention is to provide a kind of Penaeus Vannmei of increasing percent protein and preparation method thereof.
For realizing object of the present invention, the present invention adopts following technical scheme:
A feed for Penaeus Vannmei, comprises marine alga β-1,3-glucan, described marine alga β-1,3-beta-dextran content≤0.10wt%.。
Marine alga β-1,3-glucan is originated different from yeast beta-1,3-glucan, and structure is different, adds marine alga β-1,3-glucan and can significantly improve Penaeus Vannmei protein content in the feed of Penaeus Vannmei of the present invention.
Wherein, as preferably ,-1, the 3-beta-dextran content≤0.05wt% of marine alga β described in the feed of Penaeus Vannmei of the present invention.
The feed of Penaeus Vannmei of the present invention also comprises fish meal, dregs of beans, fermented bean dregs, flour, dusty yeast, squid viscera powder, the dish dregs of rice, soybean lecithin, fish oil, bentonite, calcium dihydrogen phosphate, prawn multidimensional, the many ore deposits of prawn.
Fish meal is raw material with one or more fish, the high-protein feed after processing of deoiling, dewater, pulverize.In fish meal, containing cellulose etc. is not difficult to the material that digests, and crude fat content is high, and the effective energy value of fish meal is high.Fish meal is rich in B family vitamin, especially high with vitamin B12, B2 content, also containing liposoluble vitamins such as vitamin A, D and vitamin Es.Fish meal is good mineral origin, and the content of calcium, phosphorus is very high, and ratio is suitable for, and all phosphorus is all to utilize phosphorus.The selenium content of fish meal is very high, can reach more than 2mg/kg.In addition, in fish meal, the content of iodine, zinc, iron, selenium is also very high, and containing appropriate arsenic.In addition, also containing the somatotrophic unknown factor in fish meal, this material is not also purified to compound, and this material can grow by stimulating animal.Fish meal as animal protein, amino acid ratio wherein and the amino acid needed for animal closest, be important animal protein add feed, the method that there is no replaces with other feeds.As preferably, in the feed of Penaeus Vannmei of the present invention, described fish meal accounts for described feed 25wt%.As preferably, in the feed of Penaeus Vannmei of the present invention, fish meal is preferably low temperature drying fish meal.
Further, in the feed of Penaeus Vannmei of the present invention, described fish meal is preferably the fish meal of gross protein value >=66.0%, i.e. fish meal cp66%.
Dregs of beans is a kind of byproduct obtained after soybean extracting bean oil.As a kind of high protein, dregs of beans is the primary raw material making livestock and poultry feed, can also be used for making cake food, healthy food and cosmetics and antibiotic raw material.Different according to the content of thick protein in dregs of beans, dregs of beans is divided into Three Estate, one-level dregs of beans, secondary dregs of beans and three grades of dregs of beans, is wherein most widely used with secondary dregs of beans (cp43, gross protein value >=43.0%).As preferably, dregs of beans described in the feed of Penaeus Vannmei of the present invention accounts for described feed 25.00wt%.Wherein said dregs of beans is preferably secondary dregs of beans.
Fermented bean dregs has another name called biological peptide, biological soybean meal, bioactive micro peptide, Soybean Peptide, utilize modern biological project fermented bacterium technology to combine with the solid-fermented technique of Chinese tradition, with high-quality dregs of beans for primary raw material, microbe inoculation, the ANFs in dregs of beans is eliminated to greatest extent by the fermentation of microorganism, degrading soybean protein is the little peptide protein sources of high-quality effectively, and can probio be produced, oligopeptides, glutamic acid, lactic acid, vitamin, UGF (UGF) isoreactivity material, there is raising palatability, improve nutrient digestion to absorb, growth promoting effects, reduce effect of diarrhoea.As preferably, fermented bean dregs described in the feed of Penaeus Vannmei of the present invention accounts for described feed 13wt%.
As preferably, flour described in the feed of Penaeus Vannmei of the present invention accounts for described feed 18wt%.
Dusty yeast refers to the yeast of deactivation, and product composition is deactivated yeast thalline mainly, nutritional labeling comprise still be wrapped in thalline inside crude protein, cell wall polysaccharide and abundant vitamin, auxin, trace element etc.The use cost of dusty yeast is lower.As preferably, dusty yeast described in the feed of Penaeus Vannmei of the present invention accounts for described feed 3wt%.
Squid viscera powder is that squid viscera dries the powder worn into, squid viscera powder nutritious, has strong cuttlefish internal organ raw meat fragrance, impel fish, the increase of shrimp appetite, improve the feed intake of fish, shrimp, crab, the special efficacies such as growth promoting effects are a kind of desirable aquatic feeds phagostimulants.As preferably, squid viscera powder described in the feed of Penaeus Vannmei of the present invention accounts for described feed 3wt%.
The dish dregs of rice take rapeseed as raw material, the product after carrying oil.Obtain rapeseed cake after oil is got in squeezing, after lixiviate or pre-squeezing lixiviate, obtain the dish dregs of rice.The crude protein content of the dish dregs of rice is between 34% ~ 38%, and feature is methionine content high (being only second to sesame-send cake, the dregs of rice), and lysine content is also high.And arginine content is low, be that in cake, dregs of rice feed, content is minimum.The effective energy value of rapeseed dregs is on the low side (content of starch low, Rapeseed Shell be difficult to digestibility and utilization).In mineral matter, the content of calcium and phosphorus is all high, and the content of selenium and manganese is also high.Particularly the content of selenium is the highest in conventional plant feed.Different according to the content of thick protein in the dish dregs of rice, the dish dregs of rice are divided into different brackets, the dish dregs of rice (cp 38%), the dish dregs of rice (cp 36%) and the dish dregs of rice (cp 34%).As preferably, the dish dregs of rice described in the feed of Penaeus Vannmei of the present invention account for described feed 44.50wt%.The wherein said dish dregs of rice are preferably the dish dregs of rice (cp 34%).
Soybean lecithin (Soybean phospholipids) is a kind of mixed phosphatide, it is by phosphatid ylcholine (lecithin, be called for short PC, high-grade is PPC), phosphatidyl-ethanolamine (cephalin, be called for short PE), phosphatidylinositols (lipositol, be called for short PI), phosphatidyl serine (silk amino acid phosphatide, be called for short PS) etc. becomes to be grouped into, wherein most typical is that first three is planted.Wherein lecithin or the preferably source of choline, saturated fatty acid, unrighted acid and highly unsaturated fatty acid.Soybean lecithin has excellent physiologically active and surface-active action, is a kind of efficiency natural nourishing additive agent.In feed, add soybean lecithin can play emulsification, soak, disperse and surface-active action, the nutrition such as cholamine, phosphorus, inositol, choline and aliphatic acid are provided, improve fodder energy, nutritive value, improve food conversion ratio, reduce feed coefficient; Improve palatability, there is food calling effect; Contribute to animal digesting and assimilating grease and liposoluble vitamin; Unsaturated acids in protection feed; Promote animal growth, improve the survival rate of young animal, improve aquatic livestock production performance; Prevention fatty liver, strengthens premunition; Improve the physical quality and output of granulating; Reduce feed loss and energy ezpenditure when extrusion molding; Prevent dust from flying and feed classification; Reduce the molten mistake of water-soluble nutrients in aquatic feeds; Improve drift in aquatic feeds from and sedimentation, reduce feed waste and water pollution; UGF is provided.As preferably, soybean lecithin described in the feed of Penaeus Vannmei of the present invention accounts for described feed 3wt%.
Fish oil is the grease taken out in rich fatty Fish.Fish oil is containing omega-fatty acid (omega 3), and mainly eicosapentaenoic acid (EPA) and DHA (DHA), these are the nutrients that can reduce inflammatory response.As preferably, fish oil described in the feed of Penaeus Vannmei of the present invention accounts for described feed 2wt%.
Bentonite (Bentonite) is the moisture clay pit based on montmorillonite, and chemical composition is: (Al 2, Mg 3) Si 4o 10oH 2nH 2o, bentonite contains the trace element of multiple physiological requirements, except aluminium, silicon, also containing the multiple macro-and trace-element such as Mg, Fe, K, Zn, Cu, Ca, P, Na, I, Se and bioactivator, have increase appetite, aid digestion, regulate organism balance, increase the effect such as immunity function, disease-prevention health.Additive as feed uses, and can improve the conversion ratio of feed, reduces its cost.Meanwhile, except itself having the effect of supplementary mineral nutrition, it also has good palatability, can extend the shelf-life etc. of feed significantly.As preferably, bentonite described in the feed of Penaeus Vannmei of the present invention accounts for described feed 1.95wt%.
As preferably, calcium dihydrogen phosphate described in the feed of Penaeus Vannmei of the present invention accounts for described feed 2.00wt%.
The feed of Penaeus Vannmei of the present invention is also containing prawn multidimensional.Prawn multidimensional is the mixture be made according to a certain ratio by multivitamin.As preferably, prawn multidimensional described in the feed of Penaeus Vannmei of the present invention accounts for described feed 0.1wt%.
Further, as preferably, in the feed of Penaeus Vannmei of the present invention, described prawn multidimensional every kilogram is containing vitamin A 8000IU, vitamin D 2000IU, vitamin E 50mg, vitamin K1 5mg, Cobastab 120mg, Cobastab 220mg, Cobastab 620mg, Cobastab 120.1mg, niacinamide 100mg, pantothenic acid 50mg, folic acid 10mg, biotin 0.5mg.
The feed of Penaeus Vannmei of the present invention is also containing the many ore deposits of prawn.The many ore deposits of prawn are the mixtures be made according to a certain ratio by several mineral materials.As preferably, the many ore deposits of prawn described in the feed of Penaeus Vannmei of the present invention account for described feed 0.5wt%.
Further, as preferably, in the feed of Penaeus Vannmei of the present invention, many every kilogram, the ore deposits of described prawn are containing iron 120mg, magnesium 120mg, zinc 150mg, manganese 30mg, copper 18mg, iodine 0.2mg, selenium 0.2mg, cobalt 0.1mg, chromium 0.1mg, potassium 750mg.
In some embodiments, described feed is the expanded pellet diet adopting bulking process process.
Further, in some preferred embodiments, described bulking process condition is temperature is 120 DEG C ~ 140 DEG C, 34 ~ 36 atmospheric pressure (3.4MPa ~ 3.6MPa), 23%-27% water content.Being specially expanded cavity temperature is 120 DEG C ~ 140 DEG C, 34 ~ 36 atmospheric pressure (3.4MPa ~ 3.6MPa), 23%-27% water content.
Present invention also offers a kind of preparation method of feed of Penaeus Vannmei, by marine alga β-1, the many ore deposits mixing of 3-glucan, fish meal, dregs of beans, fermented bean dregs, flour, dusty yeast, squid viscera powder, the dish dregs of rice, soybean lecithin, fish oil, bentonite, calcium dihydrogen phosphate, prawn multidimensional and prawn, adopts bulking process process and get final product; Wherein, each constituent content is fish meal 25wt%, dregs of beans 25wt%, fermented bean dregs 13wt%, flour 18wt%, dusty yeast 3wt%, squid viscera powder 3wt%, marine alga β-1,3 glucan 0.05wt%, dish dregs of rice 4wt%, soybean lecithin 3wt%, fish oil 2wt%, bentonite 1.95wt%, calcium dihydrogen phosphate 1.4wt%, prawn multidimensional 0.1wt%, prawn many ore deposits 0.5wt%.
As preferably, described bulking process condition is temperature is 120 DEG C ~ 140 DEG C, 34 ~ 36 atmospheric pressure (3.4MPa ~ 3.6MPa), 23%-27% water content.Being specially expanded cavity temperature is 120 DEG C ~ 140 DEG C, 34 ~ 36 atmospheric pressure (3.4MPa ~ 3.6MPa), 23% ~ 27% water content.
The invention provides feed of a kind of Penaeus Vannmei and preparation method thereof.The feed of Penaeus Vannmei of the present invention comprises marine alga β-1,3-glucan, described marine alga β-1,3-beta-dextran content≤0.10wt%.Add marine alga β-1,3-glucan in the feed of Penaeus Vannmei of the present invention and can significantly improve Penaeus Vannmei protein content.Experiment shows, show that the feed of Penaeus Vannmei of the present invention significantly can reduce moisture in its Body components of Penaeus Vannmei, improve its protein content (p<0.05), thus significantly improve Penaeus Vannmei protein content, improve the growth performance of Penaeus Vannmei simultaneously, reduce feed coefficient.
Detailed description of the invention
Below in conjunction with the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
In order to understand the present invention further, below in conjunction with embodiment, the present invention is described in detail.Without specified otherwise, various raw material of the present invention is commercially available prod, can be bought obtain by commercial channel.Wherein said marine alga β-1,3 glucan purchased from American Algal Scientific company, name of product is Alga muneTM (marine alga β-1,3 glucan); The described dish dregs of rice are the dish dregs of rice (cp34%); Dregs of beans is dregs of beans (cp43%); Fermented bean dregs is purchased from middle grain fodder (East Platform) Co., Ltd; Prawn multidimensional, the many ore deposits of prawn are purchased from Guangzhou Feixite Aquatic Product Technology Co., Ltd..
The feed (not containing marine alga β-1,3 glucan) of embodiment 1, Penaeus Vannmei
25wt% fish meal, 25wt% dregs of beans, 13wt% fermented bean dregs, 18wt% flour, 3wt% dusty yeast, 3wt% squid viscera powder, the 4wt% dish dregs of rice, 3wt% soybean lecithin, 2wt% fish oil, 2wt% bentonite, 1.4wt% calcium dihydrogen phosphate, 0.1wt% prawn multidimensional, the many ore deposits of 0.5wt% prawn.60 mesh sieves are crossed after pulverizing respectively, and stir and evenly mix step by step according to addition order from small to large, through twin-screw feed extruder (TSE65 type, modern foreign work machinery, Beijing, China) be expanded granulation and get final product under the condition of 120 DEG C ~ 140 DEG C, 34 ~ 36 atmospheric pressure (3.4MPa ~ 3.6MPa), 23% ~ 27% water content at expanded cavity temperature, particle diameter is 2mm; Naturally after drying, in-20 DEG C of preservations.Adopt the dry of 105 DEG C of atmosphere pressure desiccations, Kjeldahl's method, the full-cream fat determination method of acid hydrolysis, oxygen bomb calorimetry and 550 DEG C of calcination method mensuration feeds, thick protein, crude fat, total energy and ash content respectively.Detect feed chemistry and consist of dry 93.12%, thick protein 47.35%, crude fat 8.58%, total energy 20.07kJ/g and ash content 10.62%.
The feed (marine alga β-1,3 beta-dextran content 0.05wt%) of embodiment 2, Penaeus Vannmei
25wt% fish meal, 25wt% dregs of beans, 13wt% fermented bean dregs, 18wt% flour, 3wt% dusty yeast, 3wt% squid viscera powder, 0.05wt% marine alga β-1,3 glucan, the 4wt% dish dregs of rice, 3wt% soybean lecithin, 2wt% fish oil, 1.95wt% bentonite, 1.4wt% calcium dihydrogen phosphate, 0.1wt% prawn multidimensional, the many ore deposits of 0.5wt% prawn.60 mesh sieves are crossed after pulverizing respectively, and stir and evenly mix step by step according to addition order from small to large, through twin-screw feed extruder (TSE65 type, modern foreign work machinery, Beijing, China) be expanded granulation and get final product under the condition of 120 DEG C ~ 140 DEG C, 34 ~ 36 atmospheric pressure (3.4MPa ~ 3.6MPa), 23% ~ 27% water content at expanded cavity temperature, particle diameter is 2mm; Naturally after drying, in-20 DEG C of preservations.Adopt the dry of 105 DEG C of atmosphere pressure desiccations, Kjeldahl's method, the full-cream fat determination method of acid hydrolysis, oxygen bomb calorimetry and 550 DEG C of calcination method mensuration feeds, thick protein, crude fat, total energy and ash content respectively.Detect feed chemical composition with embodiment 1.
The feed (marine alga β-1,3 beta-dextran content 0.10wt%) of embodiment 3, Penaeus Vannmei
25wt% fish meal, 25wt% dregs of beans, 13wt% fermented bean dregs, 18wt% flour, 3wt% dusty yeast, 3wt% squid viscera powder, 0.10wt% marine alga β-1,3 glucan, the 4wt% dish dregs of rice, 3wt% soybean lecithin, 2wt% fish oil, 1.90wt% bentonite, 1.4wt% calcium dihydrogen phosphate, 0.1wt% prawn multidimensional, the many ore deposits of 0.5wt% prawn.60 mesh sieves are crossed after pulverizing respectively, and stir and evenly mix step by step according to addition order from small to large, through twin-screw feed extruder (TSE65 type, modern foreign work machinery, Beijing, China) be expanded granulation and get final product under the condition of 120 DEG C ~ 140 DEG C, 34 ~ 36 atmospheric pressure (3.4MPa ~ 3.6MPa), 23% ~ 27% water content at expanded cavity temperature, particle diameter is 2mm; Naturally after drying, in-20 DEG C of preservations.Adopt the dry of 105 DEG C of atmosphere pressure desiccations, Kjeldahl's method, the full-cream fat determination method of acid hydrolysis, oxygen bomb calorimetry and 550 DEG C of calcination method mensuration feeds, thick protein, crude fat, total energy and ash content respectively.Detect feed chemical composition with embodiment 1.
The feed (marine alga β-1,3 beta-dextran content 0.15wt%) of embodiment 4, Penaeus Vannmei
25wt% fish meal, 25wt% dregs of beans, 13wt% fermented bean dregs, 18wt% flour, 3wt% dusty yeast, 3wt% squid viscera powder, 0.15wt% marine alga β-1,3 glucan, the 4wt% dish dregs of rice, 3wt% soybean lecithin, 2wt% fish oil, 1.85wt% bentonite, 1.4wt% calcium dihydrogen phosphate, 0.1wt% prawn multidimensional, the many ore deposits of 0.5wt% prawn.60 mesh sieves are crossed after pulverizing respectively, and stir and evenly mix step by step according to addition order from small to large, through twin-screw feed extruder (TSE65 type, modern foreign work machinery, Beijing, China) be expanded granulation and get final product under the condition of 120 DEG C ~ 140 DEG C, 34 ~ 36 atmospheric pressure (3.4MPa ~ 3.6MPa), 23% ~ 27% water content at expanded cavity temperature, particle diameter is 2mm; Naturally after drying, in-20 DEG C of preservations.Adopt the dry of 105 DEG C of atmosphere pressure desiccations, Kjeldahl's method, the full-cream fat determination method of acid hydrolysis, oxygen bomb calorimetry and 550 DEG C of calcination method mensuration feeds, thick protein, crude fat, total energy and ash content respectively.Detect feed chemical composition with embodiment 1.
The feed (marine alga β-1,3 beta-dextran content 0.20wt%) of embodiment 5, Penaeus Vannmei
25wt% fish meal, 25wt% dregs of beans, 13wt% fermented bean dregs, 18wt% flour, 3wt% dusty yeast, 3wt% squid viscera powder, 0.20wt% marine alga β-1,3 glucan, the 4wt% dish dregs of rice, 3wt% soybean lecithin, 2wt% fish oil, 1.80wt% bentonite, 1.4wt% calcium dihydrogen phosphate, 0.1wt% prawn multidimensional, the many ore deposits of 0.5wt% prawn.60 mesh sieves are crossed after pulverizing respectively, and stir and evenly mix step by step according to addition order from small to large, through twin-screw feed extruder (TSE65 type, modern foreign work machinery, Beijing, China) be expanded granulation and get final product under the condition of 120 DEG C ~ 140 DEG C, 34 ~ 36 atmospheric pressure (3.4MPa ~ 3.6MPa), 23% ~ 27% water content at expanded cavity temperature, particle diameter is 2mm; Naturally after drying, in-20 DEG C of preservations.Adopt the dry of 105 DEG C of atmosphere pressure desiccations, Kjeldahl's method, the full-cream fat determination method of acid hydrolysis, oxygen bomb calorimetry and 550 DEG C of calcination method mensuration feeds, thick protein, crude fat, total energy and ash content respectively.Detect feed chemical composition with embodiment 1.
Embodiment 6, DIFFERENT FEED are on the impact of Penaeus Vannmei protein content
Five kinds of DIFFERENT FEED of 1, test material: embodiment 1-5.
2, test shrimp and rearing conditions
2.1, shrimp is used in test
Test Penaeus Vannmei used purchased from proving ground, Nanhai Aquatic Inst., Chinese Aquatic Scientific Research Inst Shenzhen.Before formal test, 2 weeks by a definite date raising and train is carried out, to conform to Penaeus Vannmei.Test adopts physical health, the uniform Penaeus Vannmei of Individual Size (10.84 ± 0.30g), is divided into 5 processed group at random, five kinds of DIFFERENT FEED of the embodiment 1-5 that feeds respectively, each processed group 3 repetition, each repetition 30 tail Penaeus Vannmei.Breeding cycle is 60 days, and every day, apparent being satiated with food was thrown something and fed 3 times, and the time is respectively 8:30,12:30 and 17:00.
2.2, rearing conditions
Culture experiment carries out in the indoor culture system of proving ground, Nanhai Aquatic Inst., Chinese Aquatic Scientific Research Inst Shenzhen.The every bucket volume of this system is 800L, and test water is the seawater after filtration treatment, and the every day of blowdown sooner or later once, discharges 1/3 volume water altogether.Within every 3 days, measure a water quality, dissolved oxygen >=about 7.5mg/L, ammonia nitrogen≤0.5mg/L, nitrite≤0.1mg/L, pH=7.5 ~ 8.5, cultivating system water temperature is constant temperature 27 DEG C, with heating rod and temperature controller control temperature.
3, feeding and management
Raise mid-term and raising to terminate to weigh and record to Penaeus Vannmei afterwards.Test carries out low temperature stress test to Penaeus Vannmei before the end, specific experiment scheme be test the 57th day by water temperature rapid drawdown 5 DEG C, maintain after one day; Continue rapid drawdown 5 DEG C again; Maintain one day, observe the impact on its survival rate; It is feed of normally throwing something and feeding during low temperature stress.
4, shrimp body composition analysis detects
Get 10 tail shrimps as full shrimp sample for every barrel, dry at 75 DEG C after rubbing homogenate and be used for Body components detection.105 DEG C of atmosphere pressure desiccations, Kjeldahl's method, acid hydrolysis full-cream fat determination method, oxygen bomb calorimetry and 550 DEG C of calcination methods is adopted to measure the moisture of feeds and full shrimp, thick protein, crude fat, total energy and ash content (full shrimp do not detect can) respectively.
5, computing formula
Initial counterpoise (initial body weight, IBW);
Last counterpoise (final body weight, FBW) eventually;
Heavy (g)/prawn weight gain (g) of feed coefficient (feed conversion rate, FCR)=feeding feed stuff;
Specific growth rate (specific growth rate, SGR (%d-1))=[100 × (LN (FBW)-LN (IBW))]/number of days (d);
Body weight increase rate (relative weight gain, RWG (%))=100 × prawn weightening finish (g)/initial total weight (g);
The prawn number of the prawn number of survival rate (survival rate, SR (%))=100 × survival/total;
Proteinosis rate (protein productive value, PPV (%))=100 × (shrimp end gross weight × prawn body protein content-shrimp body just gross weight × initial shrimp body protein content)/(feed intake × dietary protein level)
6, data statistics
Test data adopts mean+SD (mean ± SD) to represent, Using statistics software STATISTICA8.0 carries out two-way analysis of variance (two-way ANOVA) to test data, carrying out Multiple range test by Duncan ' s method during significant difference, take P<0.05 as significance of difference standard.The results are shown in Table 1 and table 2.
Table 1 pair Penaeus Vannmei growth performance and survival rate
Note: data are expressed as: Mean ± SD (n=3); The different lowercase alphabet of colleague's data shoulder mark shows significant difference (P<0.05).
As shown in Table 1, in the 0-32d stage, the end last counterpoise of each processed group, feed coefficient, specific growth rate, body weight increase rate are all without significant difference (p>0.05), only there is significant difference (p<0.05) in survival rate, embodiment 1 group of survival rate is significantly higher than embodiment 5 groups, but with other three processed group without significant difference (p>0.05).In the 32-60d stage, the end last counterpoise of each processed group, feed coefficient, specific growth rate, body weight increase rate and survival rate are all without significant difference (p>0.05).In the 0-60d stage, the growth performance of each processed group and survival rate all without significant difference (p>0.05), but add marine alga β-1, the 3 glucan embodiment 2 of 0.05%, the growth performance of Penaeus Vannmei increases, and feed coefficient declines to some extent.
Table 2 is on the impact of Penaeus Vannmei Body components
Project Items Embodiment 1 Embodiment 2 Embodiment 3 Embodiment 4 Embodiment 5
Moisture Moisture 75.24±0.57 ab 74.72±0.39 a 74.48±0.43 a 76.55±0.62 b 76.08±1.16 b
Crude protein CP 18.16±0.48 abc 18.60±0.07 bc 18.79±0.18 c 17.24±0.41 a 17.64±1.13 ab
Crude fat CL 0.61±0.16 0.83±0.15 0.75±0.04 0.68±0.20 0.61±0.13
Ash content Ash 3.23±0.08 3.28±0.19 3.29±0.15 2.97±0.21 3.16±0.23
Note: data are expressed as: Mean ± SD (n=3); The different lowercase alphabet of colleague's data shoulder mark shows significant difference (P<0.05).
As shown in Table 2, marine alga β-1,3 glucans on the impact of crude fat and moisture in Penaeus Vannmei Body components not significantly (p>0.05), but affect significantly (p<0.05) on the content of moisture and crude protein; Low-level interpolation marine alga β-1,3 glucan (≤0.10%) can reduce moisture in its Body components, improves its protein content, but after high level (>=0.15%) interpolation, result is on the contrary.

Claims (7)

1. a feed for Penaeus Vannmei, comprises marine alga β-1,3-glucan, described marine alga β-1,3-beta-dextran content≤0.10wt%.
2. feed according to claim 1, also comprises fish meal, dregs of beans, fermented bean dregs, flour, dusty yeast, squid viscera powder, the dish dregs of rice, soybean lecithin, fish oil, bentonite, calcium dihydrogen phosphate, prawn multidimensional, the many ore deposits of prawn.
3. feed according to claim 2, wherein each constituent content is fish meal 25wt%, dregs of beans 25wt%, fermented bean dregs 13wt%, flour 18wt%, dusty yeast 3wt%, squid viscera powder 3wt%, marine alga β-1,3 glucan 0.05wt%, dish dregs of rice 4wt%, soybean lecithin 3wt%, fish oil 2wt%, bentonite 1.95wt%, calcium dihydrogen phosphate 1.4wt%, prawn multidimensional 0.1wt%, prawn many ore deposits 0.5wt%.
4. the feed according to claim 1-3 any one, described feed is the expanded pellet diet adopting bulking process process.
5. feed according to claim 4, described bulking process condition is temperature is 120 DEG C ~ 140 DEG C, 34 ~ 36 atmospheric pressure, 23%-27% water content.
6. the preparation method of the feed of a Penaeus Vannmei, by marine alga β-1, the many ore deposits mixing of 3-glucan, fish meal, dregs of beans, fermented bean dregs, flour, dusty yeast, squid viscera powder, the dish dregs of rice, soybean lecithin, fish oil, bentonite, calcium dihydrogen phosphate, prawn multidimensional and prawn, adopts bulking process process and get final product; Wherein, each constituent content is fish meal 25wt%, dregs of beans 25wt%, fermented bean dregs 13wt%, flour 18wt%, dusty yeast 3wt%, squid viscera powder 3wt%, marine alga β-1,3 glucan 0.05wt%, dish dregs of rice 4wt%, soybean lecithin 3wt%, fish oil 2wt%, bentonite 1.95wt%, calcium dihydrogen phosphate 1.4wt%, prawn multidimensional 0.1wt%, prawn many ore deposits 0.5wt%.
7. preparation method according to claim 6, described bulking process condition is temperature is 120 DEG C ~ 140 DEG C, 34 ~ 36 atmospheric pressure, 23%-27% water content.
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CN104664148A (en) * 2015-02-05 2015-06-03 天津市晨辉饲料有限公司 Formula of expanded feed for shrimps and preparation method
CN105454734A (en) * 2016-01-05 2016-04-06 无锡肽旗生物科技有限公司 Soybean peptide protein feed used for penaeus vannamei
CN106260737A (en) * 2016-07-22 2017-01-04 天津市晨辉饲料有限公司 A kind of high-grade shrimp mixed feed and preparation method thereof
CN106260588A (en) * 2016-08-11 2017-01-04 天津市晨辉饲料有限公司 Mixed mixed feed for breeding shrimps and preparation method thereof
CN106889373A (en) * 2017-01-19 2017-06-27 浙江海洋大学 A kind of large yellow croaker phagostimulant with squid processing byproduct as primary raw material
CN107183416A (en) * 2017-07-13 2017-09-22 上海海洋大学 The immune optimization type feed for litopenaeus vannamei of fish meal is substituted with fermented bean dregs part

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CN1947552A (en) * 2006-11-03 2007-04-18 朱福康 Compound bait for juvenile white prawn and its prepn. method
CN102742729A (en) * 2012-06-20 2012-10-24 广东省农业科学院畜牧研究所 Composite immunoenhancement feed additive for litopenaeus vannamei and application and feed thereof

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JPH10313794A (en) * 1997-05-21 1998-12-02 Takeda Chem Ind Ltd Feed composition for fish kind cultivation
CN1375215A (en) * 2002-04-12 2002-10-23 中国科学院南海海洋研究所 Granular shrimp feed
JP2003334001A (en) * 2002-05-21 2003-11-25 Nutrinova Nutrition Specialties & Food Ingredients Gmbh beta-GLUCAN-CONTAINING SORBIC ACID PREPARATION TO BE USED AS FEED ADDITIVE FOR RAISING LIVESTOCK
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CN104664148A (en) * 2015-02-05 2015-06-03 天津市晨辉饲料有限公司 Formula of expanded feed for shrimps and preparation method
CN105454734A (en) * 2016-01-05 2016-04-06 无锡肽旗生物科技有限公司 Soybean peptide protein feed used for penaeus vannamei
CN106260737A (en) * 2016-07-22 2017-01-04 天津市晨辉饲料有限公司 A kind of high-grade shrimp mixed feed and preparation method thereof
CN106260588A (en) * 2016-08-11 2017-01-04 天津市晨辉饲料有限公司 Mixed mixed feed for breeding shrimps and preparation method thereof
CN106889373A (en) * 2017-01-19 2017-06-27 浙江海洋大学 A kind of large yellow croaker phagostimulant with squid processing byproduct as primary raw material
CN107183416A (en) * 2017-07-13 2017-09-22 上海海洋大学 The immune optimization type feed for litopenaeus vannamei of fish meal is substituted with fermented bean dregs part

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