Background technology
In the livestock-raising process, animal is because the various factors such as environment, feed, disease and antibiotic a large amount of uses usually cause the damage of animal intestinal and the destruction of intestinal environment, thereby cause the decline of breeding performonce fo animals, this class problem is had at present the various schemes such as the lactic acid bacteria class of use product, bacillus category, butyric acid class, oligosaccharide kind product, but effect is all not ideal enough.
The lactic acid bacteria class product because of its anaerobism, be difficult for to be preserved, and not acidproof etc. causing very difficultly in the actual use effectively reach enteron aisle, even there is enough quantity to reach enteron aisle, also mainly is to play the balance effects of microflora, a little less than the repair to impaired enteron aisle.
Common bacillus series products (as: bacillus licheniformis) although easily preserve, easily uses, and it is low that it produces the acid amount, direct regulating intestinal canal flora, and the environment that can only create for intestinal beneficial bacterium is to improve enteron aisle behind the profitable strain amount reproduction.This class bacterium is used separately, a little less than its reparation to impaired enteron aisle, the protective effect, but can promote the growth of bacillus coagulans, clostridium butyricum etc., can be that these floras are created favourable growing environment.
The butyric acid series products can directly be given the intestinal villus energy supply in theory, but in fact because butyric acid is very unstable, and easily destroyed, therefore can not well play a role, this series products is difficult to intestinal environment is played preferably regulating action simultaneously, so be local to the improvement of enteron aisle.
The oligosaccharide kind product can promote the breeding of profitable strain, but in the situation that flora destroys, onset is slower, simultaneously the reparation of impaired enteron aisle is not had direct effect.
Bacillus coagulans (but gemma is heat-resisting, acidproof etc.) can reach efficiently animal intestinal and the effects such as Pfansteihl, acetic acid, antibiotic are produced in performance, thus regulating intestinal canal environment preferably, and promote the intestinal tissue reparation;
The Pfansteihl that bacillus coagulans produces is the high efficiency energy source of clostridium butyricum, can directly promote the Growth and reproduction of clostridium butyricum and promote the clostridium butyricum synthetic butyric acid; Simultaneously, lactic acid acidity is stronger, can reduce the enteron aisle pH value, and low pH value environment can be so that the butyric acid that clostridium butyricum produces be difficult from solution, and the butyric acid of non-dissociated molecular state can better provide energy to intestinal epithelial tissue, thereby promotes the reparation of impaired enteron aisle;
Although it is the intestinal villus energy supply that clostridium butyricum can provide butyric acid, uses separately clostridium butyricum, owing to lacking lactic acid and good intestinal environment etc., makes it produce butyric acid ability and be restricted, so action effect is not remarkable;
And use separately bacillus coagulans, and although can improve preferably intestinal environment, repair being arranged also, it does not produce butyric acid, thereby the reparation of the impaired part of enteron aisle is lacked direct energy supply, and is therefore not ideal enough on impaired enteron aisle is repaired.
The flora imbalance of bacillus licheniformis capable of regulating reaches therapeutic purposes, can impel body to produce antibacterial substance, kill pathogenic bacteria, can produce the resistant activity material, and have unique biology and take the oxygen mechanism of action by force, the growth and breeding that can suppress pathogenic bacteria has good booster action to other beneficial bacteriums.
Summary of the invention
For addressing the above problem, the invention provides a kind of thalline compound and production method thereof with animal intestinal repair function, the product that the method is produced has effectively shortened the time that impaired enteron aisle recovers (reparation), reduced the generation of intestines problem, improve animal production efficiency, reduced death rate.
For achieving the above object, the present invention adopts following technology to realize, a kind of thalline compound with animal intestinal repair function, described compound mainly wherein any two or three are mixed and to form by bacillus coagulans, clostridium butyricum, three kinds of thalline of bacillus licheniformis.
A kind of production method with thalline compound of animal intestinal repair function, described compound is made as follows:
Thalline is selected: selecting viable count is 1 * 10
6CFU/g~1 * 10
12The bacillus coagulans of CFU/g, selecting viable count is 1 * 10
6CFU/g~1 * 10
12The clostridium butyricum of CFU/g, selecting viable count is 1 * 10
6CFU/g~1 * 10
12The bacillus licheniformis of CFU/g, three kinds of thalline be any two or three thalline wherein;
Material buffer mixes: bacillus coagulans, clostridium butyricum and bacillus licheniformis wherein any two or three are imported tempering tank, wherein add 0.1~5kg cushion in any two or three the mixture by every kilogram of bacillus coagulans, clostridium butyricum and bacillus licheniformis in the tempering tank again, with the common mix and blend of thalline, cushion and carrier of selecting;
Viable bacteria detects: the viable bacteria content of every gram was not less than 1 * 10 after the detection mixing was finished
4CFU/g;
Package storage: the product sealed storage is got final product at normal temperatures and pressures.
As a further improvement on the present invention, described cushion comprises water soluble starch, butyrate.
As a further improvement on the present invention, described carrier comprises corn flour, corn flour core, powdered rice hulls, defatted rice bran, zeolite powder, stone flour, wheat bran, bentonite, montmorillonite, white carbon, glucose, corn protein powder, silica, silicate, precipitated calcium carbonate, bone tankage, oyster shell whiting, kaolin, sodium sulphate.
As a further improvement on the present invention, the ratio of the mixing of thalline is 1~9.9:0.1~9 in described any two.
As a further improvement on the present invention, described three kinds of thalline mix, and bacillus coagulans accounts for 1~98% of three kinds of total viable counts of thalline, and clostridium butyricum accounts for 1~98% of three kinds of total viable counts of thalline, and bacillus licheniformis accounts for 1~98% of three kinds of total viable counts of thalline.
A kind of production method of the thalline compound with animal intestinal repair function and the compound using method that prescription obtains thereof comprise: Fodder making additive, beasts, birds and aquatic products animal intestinal protective agent.
The using method of product of the present invention comprises mixes use with a kind of thalline compound with animal intestinal repair function with feed, mixed feed viable count is not less than 1 * 10
4CFU/g, or a kind of animal intestinal put into drinking water with the thalline compound with protection and repair function, viable count also is not less than 1 * 10
4CFU/g.
The adding of cushion has effectively guaranteed the quantity of thalline inactivation in the mixed process; also reduced simultaneously the inactivation quantity of thalline in follow-up storage and the transportation; when feeding, domestic animal better guarantees the performance of thalline function; prior art middle part fractional bearer surface flatness is relatively poor; easily cause the thalline inactivation when mixing with thalline; the present invention has added cushion; Effective Raise the survival rate of thalline in mixed process; the simultaneous buffering thing has the effect that enteron aisle was protected and repaired to auxiliary thalline better faster after itself entering in the animal body, has namely reached to reduce thalline and add the fire loss ratio in man-hour and improved again the utilization ratio of animal to thalline.
The present invention compared with prior art has following beneficial effect:
A kind of thalline compound with animal intestinal repair function has shortened the time that impaired enteron aisle recovers or repairs, and has reduced the generation of intestines problem, has improved animal production efficiency.
The specific embodiment
Embodiment 1
A kind of thalline compound with animal intestinal repair function as shown in Figure 1, described compound are mainly mixed and are formed by bacillus coagulans, clostridium butyricum.
A kind of animal intestinal production method of the thalline compound with protection and repair function, described compound is made as follows:
Thalline is selected: selecting viable count is 1 * 10
9The bacillus coagulans of CFU/g, selecting viable count is 1 * 10
9The clostridium butyricum of CFU/g;
Material buffer mixes: bacillus coagulans, clostridium butyricum are imported tempering tank, press adding 3.5kg cushion in every kilogram of bacillus coagulans, the clostridium butyricum mixture again in tempering tank, with the common mix and blend of thalline, cushion and carrier of selecting;
Viable bacteria detects: the viable bacteria content of every gram was not less than 1 * 10 after the detection mixing was finished
4CFU/g;
Package storage: the product sealed storage is got final product at normal temperatures and pressures.
As a further improvement on the present invention, described cushion is water soluble starch.
As a further improvement on the present invention, described carrier be corn flour, corn flour core, powdered rice hulls, defatted rice bran, zeolite powder, stone flour, wheat bran, bentonite, montmorillonite, white carbon, glucose, corn protein powder, silica, silicate, precipitated calcium carbonate, bone tankage, oyster shell whiting, kaolin, sodium sulphate wherein any one or a few.
As a further improvement on the present invention, the ratio of the mixing of thalline is 1:1 in described any two.
A kind of production method of the thalline compound with animal intestinal repair function and the compound using method that prescription obtains thereof comprise: Fodder making additive, beasts, birds and aquatic products animal intestinal protective agent.
The using method of product of the present invention comprises mixes use with a kind of thalline compound with animal intestinal repair function with feed, mixed feed viable count is not less than 1 * 10
4CFU/g, or a kind of thalline compound with animal intestinal repair function put into drinking water, viable count also is not less than 1 * 10
4CFU/g.
Embodiment 2
A kind of thalline compound with animal intestinal repair function as shown in Figure 1, described compound are mainly mixed and are formed by bacillus coagulans, clostridium butyricum.
A kind of production method with thalline compound of animal intestinal repair function, described compound is made as follows:
Thalline is selected: selecting viable count is 1 * 10
6The bacillus coagulans of CFU/gCFU/g, selecting viable count is 1 * 10
6The clostridium butyricum of CFU/gCFU/g;
Material buffer mixes: bacillus coagulans, clostridium butyricum are imported tempering tank, press adding 0.1kg cushion in every kilogram of bacillus coagulans, the clostridium butyricum mixture again in tempering tank, with the common mix and blend of thalline, cushion and carrier of selecting;
Viable bacteria detects: the viable bacteria content of every gram was not less than 1 * 10 after the detection mixing was finished
4CFU/g;
Package storage: the product sealed storage is got final product at normal temperatures and pressures.
As a further improvement on the present invention, described cushion is butyrate.
As a further improvement on the present invention, described carrier be corn flour, corn flour core, powdered rice hulls, defatted rice bran, zeolite powder, stone flour, wheat bran, bentonite, montmorillonite, white carbon, glucose, corn protein powder, silica, silicate, precipitated calcium carbonate, bone tankage, oyster shell whiting, kaolin, sodium sulphate wherein any one or a few.
As a further improvement on the present invention, the ratio of the mixing of thalline is 1:9 in described any two.
A kind of production method of the thalline compound with animal intestinal repair function and the compound using method that prescription obtains thereof comprise: Fodder making additive, beasts, birds and aquatic products animal intestinal protective agent.
The using method of product of the present invention comprises mixes use with a kind of thalline compound with animal intestinal repair function with feed, mixed feed viable count is not less than 1 * 10
4CFU/g, or a kind of thalline compound with animal intestinal repair function put into drinking water, viable count also is not less than 1 * 10
4CFU/g.
Embodiment 3
A kind of thalline compound with animal intestinal repair function as shown in Figure 1, described compound are mainly mixed and are formed by bacillus coagulans, clostridium butyricum.
A kind of production method with thalline compound of animal intestinal repair function, described compound is made as follows:
Thalline is selected: selecting viable count is 1 * 10
12The bacillus coagulans of CFU/g, selecting viable count is 1 * 10
12The clostridium butyricum of CFU/g;
Material buffer mixes: bacillus coagulans, clostridium butyricum are imported tempering tank, press adding 5kg cushion in every kilogram of bacillus coagulans, the clostridium butyricum mixture again in tempering tank, with the common mix and blend of thalline, cushion and carrier of selecting;
Viable bacteria detects: the viable bacteria content of every gram was not less than 1 * 10 after the detection mixing was finished
4CFU/g;
Package storage: the product sealed storage is got final product at normal temperatures and pressures.
As a further improvement on the present invention, described cushion is water soluble starch.
As a further improvement on the present invention, described carrier be corn flour, corn flour core, powdered rice hulls, defatted rice bran, zeolite powder, stone flour, wheat bran, bentonite, montmorillonite, white carbon, glucose, corn protein powder, silica, silicate, precipitated calcium carbonate, bone tankage, oyster shell whiting, kaolin, sodium sulphate wherein any one or a few.
As a further improvement on the present invention, the ratio of the mixing of thalline is 9.9:0.1 in described any two.
A kind of production method of the thalline compound with animal intestinal repair function and the compound using method that prescription obtains thereof comprise: Fodder making additive, beasts, birds and aquatic products animal intestinal protective agent.
The using method of product of the present invention comprises mixes use with a kind of thalline compound with animal intestinal repair function with feed, mixed feed viable count is not less than 1 * 10
4CFU/g, or a kind of thalline compound with animal intestinal repair function put into drinking water, viable count also is not less than 1 * 10
4CFU/g.
Embodiment 4
Step is identical with embodiment 1, so different compositions mixes my 1:9 of mixed proportion at clostridium butyricum and bacillus licheniformis.
Embodiment 5
Step is identical with embodiment 1, so different compositions mixes my 99:1 of mixed proportion at clostridium butyricum and bacillus licheniformis.
Embodiment 6
Step is identical with embodiment 1, so different compositions mixes my 1:1 of mixed proportion at clostridium butyricum and bacillus licheniformis.
Embodiment 7
Step is identical with embodiment 1, so different compositions mixes my 1:1 of mixed proportion at bacillus coagulans and bacillus licheniformis.
Embodiment 8
Step is identical with embodiment 1, so different compositions mixes my 99:1 of mixed proportion at bacillus coagulans and bacillus licheniformis.
Embodiment 9
Step is identical with embodiment 1, so different compositions mixes my 1:9 of mixed proportion at bacillus coagulans and bacillus licheniformis.
Embodiment 10
A kind of thalline compound with animal intestinal repair function, described compound are mainly mixed and are formed by bacillus coagulans, clostridium butyricum, three kinds of thalline of bacillus licheniformis.
A kind of production method with thalline compound of animal intestinal repair function, described compound is made as follows:
Thalline is selected: selecting viable count is 1 * 10
9The bacillus coagulans of CFU/g, selecting viable count is 1 * 10
9The clostridium butyricum of CFU/g, selecting viable count is 1 * 10
9The bacillus licheniformis of CFU/g;
Material buffer mixes: bacillus coagulans, clostridium butyricum and bacillus licheniformis are imported tempering tank together, in tempering tank, press adding 3.5kg cushion in every kilogram of bacillus coagulans, clostridium butyricum and the bacillus licheniformis mixture again, with the common mix and blend of thalline, cushion and carrier of selecting;
Viable bacteria detects: the viable bacteria content of every gram was not less than 1 * 10 after the detection mixing was finished
4CFU/g;
Package storage: the product sealed storage is got final product at normal temperatures and pressures.
As a further improvement on the present invention, described cushion is butyrate.
As a further improvement on the present invention, described carrier be corn flour, corn flour core, powdered rice hulls, defatted rice bran, zeolite powder, stone flour, wheat bran, bentonite, montmorillonite, white carbon, glucose, corn protein powder, silica, silicate, precipitated calcium carbonate, bone tankage, oyster shell whiting, kaolin, sodium sulphate wherein one or more, described three kinds of thalline mix, bacillus coagulans accounts for 1% of three kinds of total viable counts of thalline, clostridium butyricum accounts for 98% of three kinds of total viable counts of thalline, and bacillus licheniformis accounts for 1% of three kinds of total viable counts of thalline.
A kind of production method of the thalline compound with animal intestinal repair function and the compound using method that prescription obtains thereof comprise: Fodder making additive, beasts, birds and aquatic products animal intestinal protective agent.
The using method of product of the present invention comprises mixes use with a kind of thalline compound with animal intestinal repair function with feed, mixed feed viable count is not less than 1 * 10
4CFU/g, or a kind of thalline compound with animal intestinal repair function put into drinking water, viable count also is not less than 1 * 10
4CFU/g.
Embodiment 11
A kind of thalline compound with animal intestinal repair function, described compound are mainly mixed and are formed by bacillus coagulans, clostridium butyricum, three kinds of thalline of bacillus licheniformis.
A kind of production method with thalline compound of animal intestinal repair function, described compound is made as follows:
Thalline is selected: selecting viable count is 1 * 10
6The bacillus coagulans of CFU/gCFU/g, selecting viable count is 1 * 10
6The clostridium butyricum of CFU/gCFU/g, selecting viable count is 1 * 10
6The bacillus licheniformis of CFU/gCFU/g;
Material buffer mixes: bacillus coagulans, clostridium butyricum and bacillus licheniformis are imported tempering tank together, in tempering tank, press adding 0.1kg cushion in every kilogram of bacillus coagulans, clostridium butyricum and the bacillus licheniformis mixture again, with the common mix and blend of thalline, cushion and carrier of selecting;
Viable bacteria detects: the viable bacteria content of every gram was not less than 1 * 10 after the detection mixing was finished
4CFU/g;
Package storage: the product sealed storage is got final product at normal temperatures and pressures.
As a further improvement on the present invention, described cushion is butyrate.
As a further improvement on the present invention, described carrier be corn flour, corn flour core, powdered rice hulls, defatted rice bran, zeolite powder, stone flour, wheat bran, bentonite, montmorillonite, white carbon, glucose, corn protein powder, silica, silicate, precipitated calcium carbonate, bone tankage, oyster shell whiting, kaolin, sodium sulphate wherein one or more, described three kinds of thalline mix, bacillus coagulans accounts for 1% of three kinds of total viable counts of thalline, clostridium butyricum accounts for 1% of three kinds of total viable counts of thalline, and bacillus licheniformis accounts for 98% of three kinds of total viable counts of thalline.
A kind of production method of the thalline compound with animal intestinal repair function and the compound using method that prescription obtains thereof comprise: Fodder making additive, beasts, birds and aquatic products animal intestinal protective agent.
The using method of product of the present invention comprises mixes use with a kind of thalline compound with animal intestinal repair function with feed, mixed feed viable count is not less than 1 * 10
4CFU/g, or a kind of thalline compound with animal intestinal repair function put into drinking water, viable count also is not less than 1 * 10
4CFU/g.
Embodiment 12
A kind of thalline compound with animal intestinal repair function, described compound are mainly mixed and are formed by bacillus coagulans, clostridium butyricum, three kinds of thalline of bacillus licheniformis.
A kind of production method with thalline compound of animal intestinal repair function, described compound is made as follows:
Thalline is selected: selecting viable count is 1 * 10
12The bacillus coagulans of CFU/g, selecting viable count is 1 * 10
12The clostridium butyricum of CFU/g, selecting viable count is 1 * 10
12The bacillus licheniformis of CFU/g;
Material buffer mixes: bacillus coagulans, clostridium butyricum and bacillus licheniformis are imported tempering tank together, in tempering tank, press adding 5kg cushion in every kilogram of bacillus coagulans, clostridium butyricum and the bacillus licheniformis mixture again, with the common mix and blend of thalline, cushion and carrier of selecting;
Viable bacteria detects: the viable bacteria content of every gram was not less than 1 * 10 after the detection mixing was finished
4CFU/g;
Package storage: the product sealed storage is got final product at normal temperatures and pressures.
As a further improvement on the present invention, described cushion is butyrate.
As a further improvement on the present invention, described carrier be corn flour, corn flour core, powdered rice hulls, defatted rice bran, zeolite powder, stone flour, wheat bran, bentonite, montmorillonite, white carbon, glucose, corn protein powder, silica, silicate, precipitated calcium carbonate, bone tankage, oyster shell whiting, kaolin, sodium sulphate wherein one or more, described three kinds of thalline mix, bacillus coagulans accounts for 98% of three kinds of total viable counts of thalline, clostridium butyricum accounts for 1% of three kinds of total viable counts of thalline, and bacillus licheniformis accounts for 1% of three kinds of total viable counts of thalline.
A kind of production method of the thalline compound with animal intestinal repair function and the compound using method that prescription obtains thereof comprise: Fodder making additive, beasts, birds and aquatic products animal intestinal protective agent.
The using method of product of the present invention comprises mixes use with a kind of thalline compound with animal intestinal repair function with feed, mixed feed viable count is not less than 1 * 10
4CFU/g, or a kind of thalline compound with animal intestinal repair function put into drinking water, viable count also is not less than 1 * 10
4CFU/g.