CN102630819A - Synbiotic premix for fowls and application thereof - Google Patents
Synbiotic premix for fowls and application thereof Download PDFInfo
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- CN102630819A CN102630819A CN2012100760138A CN201210076013A CN102630819A CN 102630819 A CN102630819 A CN 102630819A CN 2012100760138 A CN2012100760138 A CN 2012100760138A CN 201210076013 A CN201210076013 A CN 201210076013A CN 102630819 A CN102630819 A CN 102630819A
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- vitamin
- premix
- cobastab
- cfu
- bacillus licheniformis
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Abstract
The invention discloses synbiotic premix for fowls and application thereof. The synbiotic premix for fowls comprises synbiotics, vitamin complex and carrier. The synbiotics include bacillus licheniformis, saccharomyces cerevisiae, enterococcus faecalis and oligosaccharide. The vitamin complex comprises vitamin A, vitamin D3, vitamin E, vitamin K3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, D-biotin, niacin, folic acid and pantothenic acid. By adding the prefix into basic ration, laying rate can be increased evidently, and feed-egg ratio and broken egg rate can be increased. In addition, eggshell quality, eggshell color and hardness can be improved evidently, Haugh unit of eggs can be increased evidently, preservation length can be prolonged, and long-distance storage and transportation are benefited.
Description
Technical field
The invention belongs to field of animal feed, be specifically related to a stud bird with symphysis unit's vitamin premix and application thereof.
Background technology
Along with being rooted in the hearts of the people day by day of the strategy of sustainable development, the raising of scientific and technological progress, economic development and living standards of the people, in recent years, pollution-free food, pollution-free food have become people's consumption to be pursued.Yet in breeding process; In order to make animal products obtain certain resistance against diseases through food; Often in feed, excessively use such as additives such as antibiotic, hormones, though these materials have improved the total amount of animal products; Medicines such as a large amount of still undecomposed antibiotic, hormone just maybe also be detained in the carnivorous thing of the egg that consumers in general ate, so that human body is caused very big injury.
Summary of the invention
In order to address the above problem, the present invention provides a stud bird with symphysis unit's vitamin premix and application thereof.
Fowl provided by the invention is with the vitamin premix premix of symphysis unit; This premix contains: symphysis unit, B B-complex and carrier; Described symphysis unit comprises bacillus licheniformis (Bacillus licheniformis), saccharomyces cerevisiae (Saccharomyces cerevisiae), enterococcus faecalis (Enterococcus faecalis) and compound sugar, and said composite microbial element comprises vitamin A, vitamin D
3, vitamin E, vitamin K
3, Cobastab
1, Cobastab
2, Cobastab
6, Cobastab
12, D-biotin, nicotinic acid, folic acid and pantothenic acid.
Wherein, described bacillus licheniformis can be the various bacterial strains that this area is fit to, and is preferably CGMCC No.5094.
Wherein, described saccharomyces cerevisiae can be the various bacterial strains that this area is fit to, and is preferably CGMCC No.2388.
Wherein, described enterococcus faecalis can be the various bacterial strains that this area is fit to, and is preferably CGMCC No.5092.
Wherein, said compound sugar is an xylo-oligosaccharide.
Wherein, described carrier can be any carrier that this area is used for premix, is preferably in glucose, lactose, sucrose, soluble starch, wheat bran, defatted rice bran, powdered rice hulls, maize cob meal, corn protein powder, the stone flour one or more.
In an embodiment of invention, said premix contains compound sugar 4～10g/kg, saccharomyces cerevisiae 1～7 * 10
11Cfu/kg, bacillus licheniformis 5～10 * 10
11Cfu/kg, enterococcus faecalis 2～8 * 10
9Cfu/kg.
In embodiment preferred of the present invention, said premix contains compound sugar 7g/kg, saccharomycete 2 * 10
11Cfu/kg, bacillus 6.5 * 10
11Cfu/kg, lactic acid bacteria 4.8 * 10
8Cfu/kg.
In another embodiment of the invention, said premix contains vitamin A 0.25-0.4g/kg, vitamin D
30.1-0.2g/kg, vitamin E 0.6-0.8g/kg, vitamin K
30.05-0.08g/kg, Cobastab
10.01-0.05g/kg, Cobastab
20.07-0.11g/kg, Cobastab
60.03-0.08g/kg, Cobastab
120.05-0.09g/kg, D-biotin 0.1-0.3g/kg, nicotinic acid 0.5-1.0g/kg, folic acid 0.01-0.04g/kg and pantothenic acid 0.1-0.2g/kg.
The present invention also provides the application of described premix in improving egg fowl production performance and/or egg product matter; It is in the basal diet of egg fowl, to add described premix; Making the addition of described premix in basal diet is 0.1wt%～1wt%, preferred 0.5wt%.
In basal diet, add premix of the present invention, can significantly improve laying rate, reduce feedstuff-egg ratio and rate of broken eggs.Simultaneously, can also significantly improve eggshell quality, eggshell color and luster, hardness, can significantly improve the Ha Shi unit of egg, prolong storage period, be beneficial to long-distance storing.
Bacillus licheniformis of the present invention (Bacillus licheniformis) separates from the Roll road for the applicant, obtains through directed primary dcreening operation and multiple sieve.Its vegetative cell is shaft-like, and its gemma is oval or grows tubular, is middle life or the life of inferior end, and sporangiocyst expands slightly.Isolated or be short chain, rod end is semicircle.In 12～16h, can form bacterium colony, bacterium colony is circular, or irregular, and the edge is hair-like, and bacteria colony white is opaque, corrugationless.Gram-positive.The applicant has carried out experiments such as acid resistance, bile tolerance, heat-resisting quantity, fermentation character and bacteriostatic activity to this bacillus licheniformis, this bacillus licheniformis has characteristics such as strong to contrary environmental resistance, that adhesiveness strong, the growth fast, biomass is big, bacteriostatic activity is good.Bacillus licheniformis of the present invention (Bacillus licheniformis) applicant has been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on July 26th, 2011; Be called for short CGMCC; Address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City; Institute of Microorganism, Academia Sinica, deposit number is CGMCC No.5094.
Saccharomyces cerevisiae of the present invention (Saccharomyces cerevisiae) has anti-adversity such as good stomach juice-resistant, bile tolerance, high temperature resistant, tolerance common antibiotics and produces acid, produces enzyme, suppresses benefit such as pathogen and give birth to functions; For the applicant separates from healthy animal enteron aisle or ight soil, seed selection obtains; And be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on March 3rd, 2008; Be called for short CGMCC; The address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, preserving number is CGMCC No.2388.
Enterococcus faecalis of the present invention (Enterococcus faecalis) obtains for the applicant separates from Radix Polygalae Crotalarioidis, and its biological property is following: the bacterium colony rounding, and smooth surface, opaque, milky, the edge is smooth, gram-positive bacteria; Single thalline is spherical or ellipticity.Enterococcus faecalis of the present invention is through simulated gastric fluid, bile fluid and stable on heating screening; Again through enzymatic productivity screening and bacteriostasis screening; Can tolerate pH2.0; 1% pepsic simulated gastric fluid can tolerate 0.3% artificial bile fluid, can tolerate 85 ℃ pelleting temperature; Also can suppress enteropathogenic E K88, K99 and staphylococcus aureus, have stronger product acid and the ability that suppresses mould in dregs of beans, cotton dregs, the maize straw.Enterococcus faecalis of the present invention (Enterococcus faecalis) applicant has been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on July 26th, 2011; Be called for short CGMCC; Address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City; Institute of Microorganism, Academia Sinica, deposit number is CGMCC No.5092.
The specific embodiment
Following examples are used to explain the present invention, but are not used for limiting scope of the present invention.
Embodiment 1
This premix adopts following raw material to process: xylo-oligosaccharide 7g/kg, S. cervisiae (CGMCC No.2388) 2 * 10
11Cfu/kg, bacillus licheniformis (CGMCC No.5094) 6.5 * 10
11Cfu/kg, enterococcus faecalis CGMCC No.5092 4.8 * 10
9Cfu/kg, vitamin A 0.3g/kg, vitamin D
30.1g/kg, vitamin E 0.7g/kg, vitamin K
30.06g/kg, Cobastab
10.03g/kg, Cobastab
20.10g/kg, Cobastab
60.05g/kg, Cobastab
120.07g/kg, D-biotin 0.2g/kg, nicotinic acid 0.8g/kg, folic acid 0.03g/kg and pantothenic acid 0.2g/kg.All the other are maize cob meal.
Preparation method: above-mentioned raw materials is mixed, can obtain premix of the present invention.
Method for using: with the addition of this premix according to weight 0.5%, add in the basal diet of egg fowl, mix, routine is fed, and gets final product.
Embodiment 2
This premix adopts following raw material to process: xylo-oligosaccharide 4g/kg, S. cervisiae (CGMCC No.2388) 7 * 10
11Cfu/kg, bacillus licheniformis (CGMCC No.5094) 5 * 10
11Cfu/kg, enterococcus faecalis CGMCC No.50922 * 10
9Cfu/kg, vitamin A 0.4g/kg, vitamin D
30.1g/kg, vitamin E 0.8g/kg, vitamin K
30.05g/kg, Cobastab
10.01g/kg, Cobastab
20.07g/kg, Cobastab
60.08g/kg, Cobastab
120.09g/kg, D-biotin 0.3g/kg, nicotinic acid 1.0g/kg, folic acid 0.01g/kg and pantothenic acid 0.1g/kg.All the other are powdered rice hulls.
Preparation method: above-mentioned raw materials is mixed, can obtain premix of the present invention.
Method for using: with the addition of this premix according to weight 1.0%, add in the basal diet of egg fowl, mix, routine is fed, and gets final product.
Embodiment 3
This premix adopts following raw material to process: xylo-oligosaccharide 4g/kg, S. cervisiae (CGMCC No.2388) 4 * 10
11Cfu/kg, bacillus licheniformis (CGMCC No.5094) 10 * 10
11Cfu/kg, enterococcus faecalis CGMCC No.50928 * 10
9Cfu/kg, vitamin A 0.25g/kg, vitamin D
30.2g/kg, vitamin E 0.6g/kg, vitamin K
30.08g/kg, Cobastab
10.05g/kg, Cobastab
20.11g/kg, Cobastab
60.03g/kg, Cobastab
120.05g/kg, D-biotin 0.1g/kg, nicotinic acid 0.5g/kg, folic acid 0.04g/kg and pantothenic acid 0.2g/kg.All the other are maize cob meal.
Preparation method: above-mentioned raw materials is mixed, can obtain premix of the present invention.
Method for using: with the addition of this premix according to weight 0.7%, add in the basal diet of egg fowl, mix, routine is fed, and gets final product.
Embodiment 4
This premix adopts following raw material to process: xylo-oligosaccharide 5g/kg, S. cervisiae (CGMCC No.2388) 4 * 10
11Cfu/kg, bacillus licheniformis (CGMCC No.5094) 8 * 10
11Cfu/kg, enterococcus faecalis CGMCC No.50923 * 10
9Cfu/kg, vitamin A 0.3g/kg, vitamin D
30.2g/kg, vitamin E 0.7g/kg, vitamin K
30.06g/kg, Cobastab
10.04g/kg, Cobastab
20.11g/kg, Cobastab
60.03g/kg, Cobastab
120.05g/kg, D-biotin 0.3g/kg, nicotinic acid 0.5g/kg, folic acid 0.04g/kg and pantothenic acid 0.1g/kg.All the other are wheat bran.
Preparation method: above-mentioned raw materials is mixed, can obtain premix of the present invention.
Method for using: with the addition of this premix according to weight 0.8%, add in the basal diet of egg fowl, mix, routine is fed, and gets final product.
Test Example 1 premix is to the influence of laying hen production performance and egg product matter
1 experimental design
1.1 experimental animal and material
Choose healthy honest brown laying hen 360 plumages of egg-laying peak, age in days 265 days, 2 groups of random distribution, each handles 6 repetitions, and each repeats 30.Test was raised 10 days in advance, 60 days experimental periods, amounted to 70 days.Test group is added 0.5% premix of the present invention in corn dregs of beans type daily ration, establishing blank corn dregs of beans type daily ration is control group.
1.2 feeding and management
Routine Management program according to this test chicken house is carried out immunity and daily management, and test is carried out in same hurdle house, and feeding manner is that three layers of staged are raised in cages, and every cage is all put 3 chickens, raised for three times every day, promptly the morning 8:00, noon 12:00, afternoon 5:00.Fixedly the special messenger feeds, free choice feeding, and automatic drinking bowl drinking-water, the condition of production of observing the chicken crowd at any time, the discovery disease is in time treated.
1.3 testing index:
Production target comprises: egg number, feed consumption, egg size; Feed intake, laying rate are calculated in off-test, and each organizes average egg weight, feedstuff-egg ratio.
Egg quality index: egg size, eggshell strength, Hough unit, yolk color.
1.4 data analysis:
Adopt SPSS16.0 software to carry out data statistic analysis.Result of the test is represented with mean+SD, adopts the one-factor analysis of variance, and the relatively employing LSD multiple ratio of mean value was carried out significance test of difference between each was handled, and was significant difference with p＜0.05.
2 result of the tests
2.1 chicken is with the influence of symphysis unit vitamin premix to the laying hen production performance
Table 1 premix of the present invention is to the influence of laying hen production performance
The result shows, adds in the daily ration that premix of the present invention only can reduce day feed consumption effectively, improves average daily egg production, reduces feedstuff-egg ratio, improves laying rate, the reduction rate of broken eggs.Wherein, the most excellent with the premix effect of embodiment 1, the laying rate comparison of this premix you can well imagine high by 6.17% (P＜0.05) according to component, and the feedstuff-egg ratio comparison has reduced by 8.06% (P＜0.05) respectively according to group, and rate of broken eggs is than control group low by 73.8% (P＜0.05).
2.2 improvement to the quality of egg
The premix of table 2 embodiment of the invention 1 is to the influence of egg product matter
Can find out that from table 2 it is bigger to egg shape index, eggshell color and luster, eggshell strength, the influence of Ha Shi unit to add premix in the daily ration, compares with control group, test group eggshell color and luster has improved 6.5% (p＜0.05).
The eggshell strength test group improves 8.5% (p＜0.05) than control group.Ha Shi unit's test group has improved 6.7% (p＜0.05) than control group.Test group and control group are not all having significant difference (p＞0.05) aspect egg size, egg shape index, albumen height and the shell thickness.
Comprehensive each item index is added 0.5% chicken and is improved with symphysis unit vitamin premix group eggshell quality, and eggshell color and luster, hardness all are better than control group, and Ha Shi unit is improved significantly, and prolongs storage period, and very helps long-distance storing.
The above only is a preferred implementation of the present invention; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from know-why of the present invention; Can also make some improvement and retouching, these improvement and retouching also should be regarded as protection scope of the present invention.
Claims (10)
1. a stud bird is with symphysis unit vitamin premix; It is characterized in that; This premix contains: symphysis unit, B B-complex and carrier; Described symphysis unit comprises bacillus licheniformis (Bacillus licheniformis), saccharomyces cerevisiae (Saccharomyces cerevisiae), enterococcus faecalis (Enterococcus faecalis) and compound sugar, and said composite microbial element comprises vitamin A, vitamin D
3, vitamin E, vitamin K
3, Cobastab
1, Cobastab
2, Cobastab
6, Cobastab
12, D-biotin, nicotinic acid, folic acid and pantothenic acid.
2. premix according to claim 1 is characterized in that, described bacillus licheniformis is CGMCC No.5094.
3. premix according to claim 1 is characterized in that, described saccharomyces cerevisiae is CGMCC No.2388.
4. premix according to claim 1 is characterized in that, described enterococcus faecalis is CGMCC No.5092.
5. premix according to claim 1 is characterized in that, described compound sugar is an xylo-oligosaccharide.
6. premix according to claim 1 is characterized in that, described carrier is one or more in glucose, lactose, sucrose, soluble starch, wheat bran, defatted rice bran, powdered rice hulls, maize cob meal, corn protein powder, the stone flour.
7. according to each described premix of claim 1～6, it is characterized in that said premix contains compound sugar 4～10g/kg, saccharomyces cerevisiae 1～7 * 10
11Cfu/kg, bacillus licheniformis 5～10 * 10
11Cfu/kg, enterococcus faecalis 2～8 * 10
9Cfu/kg.
8. premix according to claim 7 is characterized in that, said premix contains compound sugar 7g/kg, saccharomyces cerevisiae 2 * 10
11Cfu/kg, bacillus licheniformis 6.5 * 10
11Cfu/kg, enterococcus faecalis 4.8 * 10
8Cfu/kg.
9. according to each described premix of claim 1～6, it is characterized in that said premix contains vitamin A 0.25-0.4g/kg, vitamin D
30.1-0.2g/kg, vitamin E 0.6-0.8g/kg, vitamin K
30.05-0.08g/kg, Cobastab
10.01-0.05g/kg, Cobastab
20.07-0.11g/kg, Cobastab
60.03-0.08g/kg, Cobastab
120.05-0.09g/kg, D-biotin 0.1-0.3g/kg, nicotinic acid 0.5-1.0g/kg, folic acid 0.01-0.04g/kg and pantothenic acid 0.1-0.2g/kg.
10. the application of each described premix of claim 1～9 in improving egg fowl production performance and/or egg product matter; It is characterized in that; In the basal diet of egg fowl, add described premix; Making the addition of described premix in basal diet is 0.1wt%～1wt%, is preferably 0.5wt%.
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| CN103082092A (en) * | 2013-01-18 | 2013-05-08 | 刘显军 | Optimal selection method of preparation parameters of bacillus licheniformis-xylo-oligosaccharide synbiotics, preparation process and application |
| CN103875893A (en) * | 2014-03-28 | 2014-06-25 | 东莞市天盛生物科技有限公司 | Multi-strain composite microorganism feed additive and preparation method thereof |
| CN103898016A (en) * | 2014-03-25 | 2014-07-02 | 华中农业大学 | High-yield lactic acid bacterium and method for preparing calcium lactate by fermenting eggshells with same |
| CN106689672A (en) * | 2017-01-04 | 2017-05-24 | 陈雨 | Synbiotics composition, synbiotics preparation, preparation method and application thereof |
| CN111838449A (en) * | 2020-06-10 | 2020-10-30 | 广东省农业科学院动物科学研究所 | Feed premix for improving egg quality and application thereof |
| US20210038656A1 (en) * | 2017-11-03 | 2021-02-11 | Nature's Sunshine Products, Inc. | Methods and compositions to enhance metabolic detoxification systems |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103082092A (en) * | 2013-01-18 | 2013-05-08 | 刘显军 | Optimal selection method of preparation parameters of bacillus licheniformis-xylo-oligosaccharide synbiotics, preparation process and application |
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| CN111838449A (en) * | 2020-06-10 | 2020-10-30 | 广东省农业科学院动物科学研究所 | Feed premix for improving egg quality and application thereof |
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