CN104286405A - Method for preparing feed by fermenting fresh soybean straws with compound probiotics - Google Patents

Method for preparing feed by fermenting fresh soybean straws with compound probiotics Download PDF

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Publication number
CN104286405A
CN104286405A CN201410572723.9A CN201410572723A CN104286405A CN 104286405 A CN104286405 A CN 104286405A CN 201410572723 A CN201410572723 A CN 201410572723A CN 104286405 A CN104286405 A CN 104286405A
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raw
soybean stalk
eats
parts
composite probiotics
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徐建雄
包健
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Shanghai Jiaotong University
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Shanghai Jiaotong University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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Abstract

The invention provides a method for preparing a feed by fermenting fresh soybean straws with compound probiotics. The preparation method comprises the following steps: mixing raw materials, namely fresh soybean straws, corn flour, wheat flour, honey, calcium formate and compound probiotics; adjusting water content; stirring to be uniform; and sealing and fermenting to obtain the product. In a process of preparing the feed by fermenting the fresh soybean straws with the compound probiotics, a fermentation process is simple and the production cost is low. The prepared fermented fresh soybean straw feed has good quality, is golden yellow and has a rich aroma, so that the food intake of cud chewers can be greatly increased. The content of lactobacillus and lactic acid in the fermented feed is high and the pH value is low; and the recovery rate of dry matters is very high, and the content of trypsin inhibiting factors and the urease activity are low.

Description

Composite probiotics ferment eats the method that soybean stalk prepares feed raw
Technical field
The present invention relates to the preparation method of the feed of technical field of livestock husbandry, specifically, relate to a kind of composite probiotics ferment and eat the method that soybean stalk prepares feed raw.
Background technology
China is populous nation, being in great demand to meat, is necessary to greatly develop animal husbandry, but Herbivores feed price is expensive for a long time, directly causes feeding cost too high, thus have impact on the economic benefit of livestock and poultry cultivation.The various potential feed resource of development and utilization is of great significance development China animal husbandry tool.
All kinds of agricultural straw resource of China is very abundant.According to estimates, the annual agricultural crop straw output of China reaches more than 700,000,000 ton, accounts for 30% of world's stalk total output.But the utilization of resources is also unreasonable, according to preliminary investigation, stalk overall utilization is only tens percent.The overwhelming majority idle stacking of stalk or burning disposal, not only cause the great wasting of resources and serious environmental pollution, but also cause the frequent generation of fire and traffic accident.
Stalk is huge potential feed resource, can Direct-fed ruminant, also can as the raw material of animal and fowl fodder.The agricultural byproduct such as stalk are used as feed and not only can reduce environmental pollution, also can turn waste into wealth, solve the problems such as current feed resource is not enough, expensive.
Beanstalk is the byproduct of beans, and annual beanstalk output reaches 1,500 ten thousand tons.Beanstalk resource long-term does not obtain rationally effective development and utilization, and the beanstalk of about 2/3 is incinerated, and causes the wasting of resources and environmental pollution.The main cause that beanstalk can not be used as feed is well that quality is thick and stiff, palatability is poor and be of low nutritive value.Generally adopt the method process unconventional water resources such as hydrolysis, expanded, enzyme in production, because process technology is immature, part processing mode destroys the nutritive value of feed, and processing cost is high, lacks competitiveness.
Utilize composite probiotic fermented feed can not only improve the palatability of feed, improve the digestibility of feed, have additional nutrients the absorption of material, and can growth of animal be promoted, regulate gastrointestinal bacterial flora, livestock and poultry fermented feed containing probiotics of feeding can improve production performance and resistance against diseases, can also reduce antibiotic use, for the mankind provide healthy and safe animal product.
At present, less for the research eating soybean stalk fermented feed raw, general fermented feed does not adopt compound probiotic yet, and fermentation efficiency is low.
Summary of the invention
For defect of the prior art, the invention provides a kind of composite probiotics ferment and eat the method that soybean stalk prepares feed raw.The present invention adopts the technique of microbial solid anaerobic fermentation, improves and eats soybean stalk Forage quality raw.The preparation process of composite probiotic fermented feed is without the need to raw material sterilizing, and zymotechnique is simple, and production cost is low.Soybean stalk feed fine texture is eaten in fermentation prepared by the present invention raw, in golden yellow, have strong fragrance, greatly can improve the feed intake of ruminant.In fermented feed lactic acid bacteria and lactic acid content high, pH value is low, and the dry rate of recovery is very high, trypsin ihhibitor content and urease activity low.
The present invention is achieved by the following technical solutions:
The present invention relates to a kind of composite probiotics ferment and eat the method that soybean stalk prepares feed raw, described preparation method comprises the steps: to eat soybean stalk raw, corn flour, flour by comprising, molasses, calcium formate, the raw material mixing of compound probiotic, regulates moisture, stir, sealing, fermentation.
Preferably, described raw material comprises eats soybean stalk raw, corn flour, flour, molasses, calcium formate, and the parts by weight of compound probiotic are as follows:
More preferably, described raw material comprises eats soybean stalk raw, corn flour, flour, molasses, calcium formate, the parts by weight of compound probiotic mixing are as follows: eat soybean stalk 776 parts raw, corn flour 100 parts, flour 40 parts, 30 parts, molasses, calcium formate 4 parts, compound probiotic 50 parts.
Preferably, soybean stalk is eaten described in raw for front pulverizing of fermenting.
Preferably, the particle diameter eating soybean stalk raw after described pulverizing is 4 ~ 10mm.
More preferably, the particle diameter eating soybean stalk raw after described pulverizing is 5mm.
Preferably, described moisture regulates and specifically refers to the moisture weight content of raw material to be adjusted to 25% ~ 45%.
More preferably, described moisture regulates and specifically refers to the moisture weight content of raw material to be adjusted to 35%.
Preferably, the condition of described fermentation is: fermentation temperature is 25 ~ 35 DEG C, and fermentation time is 15 ~ 35 days.
Preferably, the condition of described fermentation is: fermentation temperature is 30 DEG C, and fermentation time is 30 days.
Preferably, described compound probiotic is Bacillus acidi lactici, bacillus subtilis, saccharomycete mass ratio be the mixture of 5:1:3.
Compared with prior art, the present invention has following beneficial effect:
(1) the inventive method adopts the technique of microbial solid anaerobic fermentation, and the raw material adopted is without the need to sterilizing, and zymotechnique is simple, and production cost is low;
(2) soybean stalk feed fine texture is eaten in the fermentation that prepared by the inventive method raw, in golden yellow, have strong fragrance, greatly can improve the feed intake of ruminant;
(3) in the fermented feed prepared of the inventive method lactic acid bacteria and lactic acid content high, pH value is low, and the dry rate of recovery is high, and trypsin ihhibitor content and urease activity are extremely low.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in detail.Following examples will contribute to those skilled in the art and understand the present invention further, but not limit the present invention in any form.It should be pointed out that to those skilled in the art, without departing from the inventive concept of the premise, some distortion and improvement can also be made.These all belong to protection scope of the present invention.
Number in below implementing refers to parts by weight; Soybean stalk of eating raw described in enforcement is pluck the residue after eating soybean pod raw; The Bacillus acidi lactici adopted in example, bacillus subtilis, saccharomycete are purchased from Shanghai Chuangbo Ecological Engineering Co., Ltd.
embodiment 1
The present embodiment relates to a kind of composite probiotics ferment and eats the method that soybean stalk prepares feed raw, and described method comprises the steps:
Choose pulverize for 4mm eat soybean stalk 787 parts raw, corn flour 100 parts, flour 40 parts, 30 parts, molasses, calcium formate 3 parts, compound probiotic 40 parts (Bacillus acidi lactici, bacillus subtilis, saccharomycete mass ratio are 5:1:3), regulate moisture to 45%, stir, load in Fresco Bag, 35 DEG C of fermentations obtain finished product feedstuff in 30 days.
The implementation result of the present embodiment: finished product feedstuff fine texture prepared by the present embodiment, color is golden yellow, and have fragranced, crude protein content is 13.80%, and total bacteria count is 3.20 × 10 9cfumL -1, lactic acid bacteria number is 2.33 × 10 9cfumL -1, pH is 4.81, and the dry rate of recovery is 98.70%, and trypsin ihhibitor content is 0.01mgg -1, urease activity does not detect.
embodiment 2
The present embodiment relates to a kind of composite probiotics ferment and eats the method that soybean stalk prepares feed raw, and described method comprises the steps:
Choose pulverize for 5mm eat soybean stalk 755 parts raw, corn flour 10 parts, flour 10 parts, 50 parts, molasses, calcium formate 5 parts, compound probiotic 70 parts (Bacillus acidi lactici, bacillus subtilis, saccharomycete mass ratio are 5:1:3), regulate moisture to 35%, stir, load in Fresco Bag, 30 DEG C of fermentations obtain finished product feedstuff in 15 days.
The present embodiment implementation result: finished product feedstuff fine texture prepared by the present embodiment, color is golden yellow, and have fragranced, crude protein content is 13.23%, and total bacteria count is 2.07 × 10 9cfumL -1, lactic acid bacteria number is 1.81 × 10 9cfumL -1, pH is 4.87, and the dry rate of recovery is 99.07%, and trypsin ihhibitor content is 0.01mgg -1, urease activity does not detect.
embodiment 3
The present embodiment relates to a kind of composite probiotics ferment and eats the method that soybean stalk prepares feed raw, and described method comprises the steps:
Choose pulverize for 8mm eat soybean stalk 646 parts raw, corn flour 200 parts, flour 100 parts, 20 parts, molasses, calcium formate 4 parts, compound probiotic 30 parts (Bacillus acidi lactici, bacillus subtilis, saccharomycete mass ratio are 5:1:3), regulate moisture to 25%, stir, load in Fresco Bag, 25 DEG C of fermentations obtain finished product feedstuff in 15 days.
The implementation result of the present embodiment: finished product feedstuff fine texture prepared by the present embodiment, color is glassy yellow, savory, and crude protein content is 14.40%, and total bacteria count is 1.67 × 10 9cfumL -1, lactic acid bacteria number is 1.49 × 10 9cfumL -1, pH is 4.93, and the dry rate of recovery is 99.07%, and trypsin ihhibitor content is 0.02mgg -1, urease activity is 0.01mgg -1min -1.
embodiment 4
The present embodiment relates to a kind of composite probiotics ferment and eats the method that soybean stalk prepares feed raw, and described method comprises the steps:
Choose pulverize for 10mm eat soybean stalk 699 parts raw, corn flour 40 parts, flour 200 parts, 10 parts, molasses, calcium formate 1 part, compound probiotic 50 parts (Bacillus acidi lactici, bacillus subtilis, saccharomycete mass ratio are 5:1:3), regulate moisture to 40%, stir, load in Fresco Bag, 30 DEG C of fermentations obtain finished product feedstuff in 35 days.
The implementation result of the present embodiment: finished product feedstuff fine texture prepared by the present embodiment, color is golden yellow, and have fragranced, crude protein content is 13.68%, and total bacteria count is 3.25 × 10 9cfumL -1, lactic acid bacteria number is 2.28 × 10 9cfumL -1, pH is 4.84, and the dry rate of recovery is 98.66%, and trypsin ihhibitor content is 0.01mgg -1, urease activity does not detect.
embodiment 5
The present embodiment relates to a kind of composite probiotics ferment and eats the method that soybean stalk prepares feed raw, described method is identical with embodiment 4, optimize part to be: described raw material comprises eats soybean stalk raw, corn flour, flour, molasses, calcium formate, the parts by weight of compound probiotic mixing are as follows: eat soybean stalk 776 parts raw, corn flour 100 parts, flour 40 parts, 30 parts, molasses, calcium formate 4 parts, compound probiotic 50 parts (Bacillus acidi lactici, bacillus subtilis, saccharomycete mass ratio are 5:1:3); Fermentation temperature is 30 DEG C, and fermentation time is 30 days.
The beneficial effect of the present embodiment: finished product feedstuff fine texture prepared by the present embodiment, color is golden yellow, and have fragranced, crude protein content is 14%, and total bacteria count is 3.55 × 10 9cfumL -1, lactic acid bacteria number is 2.68 × 10 9cfumL -1, pH is 4.84, and the dry rate of recovery is 99%, and trypsin ihhibitor content is 0.01mgg -1, urease activity does not detect.
comparative example 1
This comparative example is the comparative example of embodiment 4, and a kind of composite probiotics ferment eats the method that soybean stalk prepares feed raw, described method is identical with embodiment 4, and difference is, in compound probiotic, Bacillus acidi lactici, bacillus subtilis, saccharomycete mass ratio are the mixture of 3:1:1.
The implementation result of this comparative example: finished product feedstuff fine texture prepared by this comparative example, color is golden yellow, and have fragranced, crude protein content is 13.68%, and total bacteria count is 2.25 × 10 8cfumL -1, lactic acid bacteria number is 2.18 × 10 8cfumL -1, pH is 4.24, and the dry rate of recovery is 88.96%, and trypsin ihhibitor content is 0.01mgg -1, urease activity does not detect.
comparative example 2
This comparative example is the comparative example of embodiment 4, and a kind of composite probiotics ferment eats the method that soybean stalk prepares feed raw, described method is identical with embodiment 4, difference is, compound probiotic is: purchased from Shanghai Chuangbo Ecological Engineering Co., Ltd, lot number: 080825, main component is lactic acid bacteria, bacillus subtilis, saccharomycete etc., and wherein viable count is more than or equal to 50 × 10 8cfu/ml, lactic acid bacteria number 12 × 10 8cfu/ml.
The implementation result of this comparative example: finished product feedstuff fine texture prepared by this comparative example, color is golden yellow, and have fragranced, crude protein content is 8.68%, and total bacteria count is 1.55 × 10 8cfumL -1, lactic acid bacteria number is 1.38 × 10 8cfumL -1, pH is 5.8, and the dry rate of recovery is 77.86%, and trypsin ihhibitor content is 0.01mgg -1, urease activity does not detect.
comparative example 3
This comparative example is the comparative example of embodiment 4, and a kind of composite probiotics ferment eats the method that soybean stalk prepares feed raw, described method is identical with embodiment 4, difference is, raw material comprises following content: choose pulverize for 10mm eat soybean stalk 855 parts raw, corn flour 5 parts, flour 250 parts, 3 parts, molasses, calcium formate 10 parts, compound probiotic 10 parts, regulate moisture to 40%, stir, load in Fresco Bag, 30 DEG C of fermentations obtain finished product feedstuff in 35 days.
The implementation result of this comparative example: finished product feedstuff fine texture prepared by this comparative example, color is brown color, has fragranced, and crude protein content is 9.57%, and total bacteria count is 1.25 × 10 8cfumL -1, lactic acid bacteria number is 1.03 × 10 8cfumL -1, pH is 6, and the dry rate of recovery is 85%, and trypsin ihhibitor content is 0.01mgg -1, urease activity does not detect.
comparative example 4
This comparative example is the comparative example of embodiment 4, relate to a kind of composite probiotics ferment and eat the method that soybean stalk prepares feed raw, difference is: after the raw material identical with embodiment component and content and moisture being stirred, and loads in Fresco Bag, and 35 DEG C of fermentations obtain finished product feedstuff in 10 days.
The implementation result of this comparative example: finished product feedstuff fine texture prepared by this comparative example, color is brown color, has fragranced, and crude protein content is 5.57%, and total bacteria count is 1.37 × 10 8cfumL -1, lactic acid bacteria number is 1.25 × 10 8cfumL -1, pH is 6.5, and the dry rate of recovery is 90%, and trypsin ihhibitor content is 0.01mgg -1, urease activity does not detect.
Above specific embodiments of the invention are described.It is to be appreciated that the present invention is not limited to above-mentioned particular implementation, those skilled in the art can make various distortion or amendment within the scope of the claims, and this does not affect flesh and blood of the present invention.

Claims (10)

1. composite probiotics ferment eats the method that soybean stalk prepares feed raw, it is characterized in that, described preparation method comprises the steps: to eat soybean stalk raw by comprising, corn flour, flour, molasses, calcium formate, the raw material mixing of compound probiotic, regulate moisture, stir, sealing, fermentation;
Described raw material comprises eats soybean stalk raw, corn flour, flour, molasses, calcium formate, and the parts by weight of compound probiotic are as follows:
2. composite probiotics ferment according to claim 1 eats the method that soybean stalk prepares feed raw, it is characterized in that, described raw material comprises eats soybean stalk raw, corn flour, flour, molasses, calcium formate, the parts by weight of compound probiotic mixing are as follows: eat soybean stalk 776 parts raw, corn flour 100 parts, flour 40 parts, 30 parts, molasses, calcium formate 4 parts, compound probiotic 50 parts.
3. composite probiotics ferment according to claim 1 and 2 eats the method that soybean stalk prepares feed raw, it is characterized in that, described in eat soybean stalk raw for front pulverizing of fermenting.
4. composite probiotics ferment according to claim 3 eats the method that soybean stalk prepares feed raw, it is characterized in that, the particle diameter eating soybean stalk raw after described pulverizing is 4 ~ 10mm.
5. composite probiotics ferment according to claim 4 eats the method that soybean stalk prepares feed raw, it is characterized in that, the particle diameter eating soybean stalk raw after described pulverizing is 5mm.
6. composite probiotics ferment according to claim 1 eats the method that soybean stalk prepares feed raw, it is characterized in that, described moisture regulates and specifically refers to the moisture weight content of raw material to be adjusted to 25% ~ 45%.
7. composite probiotics ferment according to claim 6 eats the method that soybean stalk prepares feed raw, it is characterized in that, described moisture regulates and specifically refers to the moisture weight content of raw material to be adjusted to 35%.
8. composite probiotics ferment according to claim 1 eats the method that soybean stalk prepares feed raw, it is characterized in that, the condition of described fermentation is: fermentation temperature is 25 ~ 35 DEG C, and fermentation time is 15 ~ 35 days.
9. composite probiotics ferment according to claim 8 eats the method that soybean stalk prepares feed raw, it is characterized in that, the condition of described fermentation is: fermentation temperature is 30 DEG C, and fermentation time is 30 days.
10. composite probiotics ferment according to claim 1 eats the method that soybean stalk prepares feed raw, it is characterized in that, described compound probiotic is Bacillus acidi lactici, bacillus subtilis, saccharomycete mass ratio are the mixture of 5:1:3.
CN201410572723.9A 2014-10-23 2014-10-23 Method for preparing feed by fermenting fresh soybean straws with compound probiotics Pending CN104286405A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104605163A (en) * 2015-02-12 2015-05-13 黑龙江省聚拢乾坤农业技术开发有限公司 High-calcium straw feed formula and production method thereof
CN110403073A (en) * 2019-08-26 2019-11-05 厦门市民安创享实业有限公司 A kind of soybean stalk fermented feed and preparation method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1663422A (en) * 2005-04-19 2005-09-07 单德章 Zymophyte of plant straw feedstuff and its preparation
CN101999551A (en) * 2010-12-08 2011-04-06 上海市奶牛研究所 Total mixed ration (TMR) fermented feed for cow replacement cattle using soybean straws as raw materials and preparation thereof
CN103098980A (en) * 2013-01-28 2013-05-15 西北农林科技大学 Microorganism straw feed fermenting agent

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1663422A (en) * 2005-04-19 2005-09-07 单德章 Zymophyte of plant straw feedstuff and its preparation
CN101999551A (en) * 2010-12-08 2011-04-06 上海市奶牛研究所 Total mixed ration (TMR) fermented feed for cow replacement cattle using soybean straws as raw materials and preparation thereof
CN103098980A (en) * 2013-01-28 2013-05-15 西北农林科技大学 Microorganism straw feed fermenting agent

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104605163A (en) * 2015-02-12 2015-05-13 黑龙江省聚拢乾坤农业技术开发有限公司 High-calcium straw feed formula and production method thereof
CN110403073A (en) * 2019-08-26 2019-11-05 厦门市民安创享实业有限公司 A kind of soybean stalk fermented feed and preparation method thereof

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Application publication date: 20150121