CN113558138A - Feed additive for improving intestinal health and anti-stress capability of livestock and poultry - Google Patents

Feed additive for improving intestinal health and anti-stress capability of livestock and poultry Download PDF

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Publication number
CN113558138A
CN113558138A CN202010347855.7A CN202010347855A CN113558138A CN 113558138 A CN113558138 A CN 113558138A CN 202010347855 A CN202010347855 A CN 202010347855A CN 113558138 A CN113558138 A CN 113558138A
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percent
feed additive
livestock
poultry
improving
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孙标
韩立虎
张富明
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Zhejiang Qirun Biological Technology Co ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/105Aliphatic or alicyclic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/116Heterocyclic compounds
    • A23K20/137Heterocyclic compounds containing two hetero atoms, of which at least one is nitrogen
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/174Vitamins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/28Silicates, e.g. perlites, zeolites or bentonites

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physiology (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Inorganic Chemistry (AREA)
  • Fodder In General (AREA)

Abstract

The invention relates to a feed additive for improving intestinal health of livestock and poultry and improving anti-stress capability, which comprises 1.0-2.0% of bacillus licheniformis, 3.0-4.0% of bacillus subtilis, 2.0-3.0% of bifidobacterium and 1.0-2.0% of enterococcus faecalis according to weight percentage; 3.0 to 4.0 percent of honeysuckle extract, 3.0 to 4.0 percent of astragalus extract and 1.0 to 2.0 percent of liquorice extract; 0.5 to 1.0 percent of vitamin C; 0.5 to 1.0 percent of tea polyphenol; 2.0 to 4.0 percent of sodium humate; 65.0 to 70.0 percent of montmorillonite; 1.0 to 2.0 percent of zymosan; 2.0 to 4.0 percent of gamma-aminobutyric acid. The feed additive provided by the invention has the advantages that the components are reasonably proportioned, the effects of the components are fully exerted, the growth performance of livestock and poultry can be well improved, the intestinal health is improved, the damage of stress to organisms is reduced, and the breeding benefit can be obviously improved.

Description

Feed additive for improving intestinal health and anti-stress capability of livestock and poultry
Technical Field
The invention belongs to the technical field of feed additives, and particularly relates to a feed additive for improving intestinal health and anti-stress capability of livestock and poultry.
Background
The fact that bacillus coagulans is formally listed in feed additive variety catalog in the department of agriculture in 12 months in 2013, the feed microecologics for feeds used in the feed additive industry and the breeding industry in China are up to 34, and 18 new probiotics are added compared with the 2008 edition is fully explained: under the industry development trend of 'resistance reduction and resistance prohibition', the feeding microecological preparation is one of the main directions of research, development and popularization of safe and efficient feed additives in China.
Although the development of the microecological preparation as an emerging feed additive is fast in the past decade, the use effect of the microecological preparation is obviously unstable and discontinuous. This is mainly due to the fact that scientists have little knowledge about the structure and characteristics of intestinal microorganisms in normal and pathological states of animals and their knowledge about the interaction between intestinal microorganisms and hosts is almost blank. Only through the more intensive research on the functions of the animal intestinal microbial flora in the growth, development and health care of animals can the aim be achieved, and the novel microecological preparation is ensured to be more effective, safe and targeted.
In the current microecological preparation products, the initial source of the bacterial strains is probably not the animal intestinal tract, so that whether the bacterial strains adapt to the animal intestinal tract environment or successfully pass through the gastrointestinal tract or not and play a role in the microenvironment cannot be determined, and meanwhile, most products are not coated, and are not resistant to storage, high temperature and gastric acid and bile.
The development of green feed additives is trendy, and when probiotics are researched, the characteristics of animal intestinal bacteria flora and the relation between the animal intestinal bacteria flora and hosts and the environment must be studied vigorously to design a high-efficiency new-generation microecological preparation. The livestock and poultry are stressed under the influence of various factors in the growth process, the feed intake is reduced, the in vivo synthesis capacity is reduced, the immune effect is inhibited, the resistance is reduced, and a series of changes of nutrition and physiological metabolism of the livestock and poultry are caused by the stress, so that the production loss of the animal husbandry is immeasurable.
The invention provides a feed additive for improving the intestinal health of livestock and poultry and improving the anti-stress capability, which adopts a mode of jointly regulating and controlling a microecological preparation and a plant extract to condition and maintain the intestinal health of animals and enhance the autoimmune function of organisms. The microecological preparation can regulate microecological flora in animal intestinal tract, increase content of beneficial bacteria such as lactobacillus, bifidobacterium, bacillus subtilis and the like, effectively inhibit growth of pathogenic bacteria, and the plant extract can achieve the effects of regulating center, clearing heat and detoxicating, cooling blood and stopping dysentery, tonifying qi and promoting fluid, promoting appetite, enhancing physique, brightening hair color, ruddy skin color, being good for sleeping, robust in growth and remarkably reducing morbidity. Improving the adaptive capacity and the regulating capacity of the livestock and poultry to the internal environment.
The feed additive for improving the intestinal health of livestock and poultry and improving the anti-stress capability combines the components of plant extract, microecological preparation, sodium humate, montmorillonite, biological polyphenol, vitamins and the like, can replace antibiotics, effectively ensures the intestinal health and improves the production performance. Can improve fat digestibility, regulate intestinal flora balance, and protect intestinal health.
The product of the invention is beneficial to the healthy development of livestock and poultry breeding industry in China, and has wide market application prospect.
Disclosure of Invention
The invention aims to provide the feed additive for improving the intestinal health of livestock and poultry and improving the anti-stress capability, and the feed additive can improve the intestinal health of the livestock and poultry, improve the production performance of the livestock and poultry and reduce the risk hazard of stress to livestock and poultry breeding.
The feed additive provided by the invention has the advantages of improving the intestinal health of livestock and poultry and improving the anti-stress capability, along with reasonable formula design, and remarkable effects of improving the growth performance of the livestock and poultry and reducing stress hazard.
In order to achieve the purpose, the formula of the invention mainly comprises a microecological preparation, a plant extract, vitamin C, biological polyphenol, ore, zymosan and gamma-aminobutyric acid.
The microecological preparation comprises bacillus licheniformis, bacillus subtilis, bifidobacterium and enterococcus faecalis.
The plant extract comprises honeysuckle extract, astragalus extract and liquorice extract.
The biological polyphenol is tea polyphenol.
The ore consists of sodium humate and nano montmorillonite.
The montmorillonite is nano montmorillonite with the granularity of 325 meshes.
The invention has the beneficial effects that:
the feed additive reduces the influence on animal breeding caused by the decrease of feed intake, the decrease of immunity resistance and the decrease of synthesis capacity due to stress, can quickly get rid of animals from a stress state, and can regulate the intestinal health of livestock and poultry, improve the detoxification capacity of liver, improve immunity, increase feed intake, improve feeding efficiency and improve production performance.
Detailed Description
The following describes in detail specific embodiments of the present invention with reference to specific examples.
The bacillus licheniformis, the bacillus subtilis, the bifidobacterium and the enterococcus faecalis in the formula belong to beneficial probiotic groups, the bacillus licheniformis, the bacillus subtilis, the bifidobacterium and the enterococcus faecalis are used in the invention, the digestion and absorption of nutrient substances can be facilitated, the probiotic metabolism can generate nutrient substances such as vitamins, short-chain fatty acids, antioxidants, amino acids and the like which are necessary for the growth of animals, the growth of harmful bacteria can be inhibited, the toxin generated by the harmful bacteria can be eliminated, and the harm of the toxin to organisms can be resisted.
The honeysuckle extract, the astragalus extract and the liquorice extract in the formula have the effects of enhancing immunity, improving the detoxifying capability of the liver, resisting bacteria, resisting stress and the like.
The preparation method of the honeysuckle extract comprises the steps of taking honeysuckle as a raw material, crushing the honeysuckle by using a crusher, sieving by using a 25-mesh sieve, extracting chlorogenic acid in honeysuckle leaves by using 200W ultrasonic wave in an auxiliary manner, wherein the material-liquid ratio is 1: 10. the extraction temperature is 55 deg.C, the extraction time is 60min, and the ethanol concentration is 40%.
The preparation method of the licorice extract comprises the steps of taking licorice as a raw material, crushing the licorice by using a crusher, sieving the crushed licorice by using a 25-mesh sieve, taking 4-6 times of 50-60% ethanol solution as an extraction medium, extracting for 0.5-1.5 h for 1-3 times, and taking the content of glycyrrhizic acid as a detection index.
The preparation method of the astragalus extract comprises the steps of taking astragalus as a raw material, extracting astragalus polysaccharide, flavone and saponin active ingredients by using a boiling water bath for 2-4 times, combining extracting solutions, adjusting the pH of the extracting solution to 7.0 by using a 1.0mol/L NaOH solution, removing precipitates, adjusting the pH of the extracting solution to 1.0-2.0 by using a 1.0mol/L HCl solution, collecting the precipitates, washing the precipitates by using distilled water and 10-40% ethanol respectively, and drying to obtain the astragalus extract.
The vitamin C in the formula has the effects of resisting oxidation, removing free radicals in vivo and reducing the attack of the free radicals on organs in vivo.
The tea polyphenol in the formula has the effects of scavenging free radicals, resisting bacteria, sterilizing and the like.
The sodium humate and the nano montmorillonite in the invention belong to ore feed additives, have the effects of buffering the acidity change of animal digestive tracts and improving the activity of digestive enzymes, thereby enhancing the appetite and being beneficial to the digestion and absorption of feed nutrients; adsorbing toxic and harmful substances in intestinal tract, and improving body condition; inhibiting oxidation and preventing deactivation of vitamin additives; promoting the growth and reproduction of beneficial microorganisms in rumen of ruminant.
The zymosan in the formula is an extract of beer yeast and baker's yeast, and has the effects of regulating immunity, resisting oxidation, relaxing bowel, regulating gastrointestinal functions, inhibiting bacterial proliferation and the like.
The gamma-aminobutyric acid in the formula has the effects of tranquilizing nerves, resisting anxiety, reducing neuron activity and preventing nerve cells from being overheated.
The feed additive comprises the following raw materials in formula ratio: 1.0-2.0% of bacillus licheniformis, 3.0-4.0% of bacillus subtilis, 2.0-3.0% of bifidobacterium and 1.0-2.0% of enterococcus faecalis; 3.0 to 4.0 percent of honeysuckle extract, 3.0 to 4.0 percent of astragalus extract and 1.0 to 2.0 percent of liquorice extract; 0.5 to 1.0 percent of vitamin C; 0.5 to 1.0 percent of tea polyphenol; 2.0 to 4.0 percent of sodium humate; 65.0 to 70.0 percent of montmorillonite; 1.0 to 2.0 percent of zymosan; 2.0 to 4.0 percent of gamma-aminobutyric acid.
Example 1
The feed additive for improving the intestinal health and the anti-stress capability of livestock and poultry comprises the following components in percentage by mass: 1.0% of bacillus licheniformis, 3.0% of bacillus subtilis, 4.0% of bifidobacterium and 2.0% of enterococcus faecalis; 3.0% of honeysuckle extract, 3.0% of astragalus extract and 2.0% of liquorice extract; 0.5 percent of vitamin C; 0.5 percent of tea polyphenol; 4.0% of sodium humate; montmorillonite 70.0%; 1.0% of zymosan; 2.0% of gamma-aminobutyric acid is placed in a feed additive premixer, fully stirred for 180s, and after the uniform mixing is completed, the feed additive is subpackaged according to 1kg per package by adopting full-automatic quantification, and is packaged and sealed by adopting a company general feed additive packaging bag, so that the feed additive for improving the intestinal health of livestock and poultry and improving the anti-stress capability is obtained.
Example 2
The feed additive for improving the intestinal health and the anti-stress capability of livestock and poultry comprises the following components in percentage by mass: 2.0% of bacillus licheniformis, 4.0% of bacillus subtilis, 3.0% of bifidobacterium and 1.0% of enterococcus faecalis; honeysuckle extract 4.0%, astragalus root extract 4.0%, licorice root extract 1.0%; 1.0% of vitamin C; 1.0% of tea polyphenol; 2.0% of sodium humate; 65.0% of montmorillonite; 1.0% of zymosan; 2.0% of gamma-aminobutyric acid is placed in a feed additive premixer, fully stirred for 180s, and after the uniform mixing is completed, the feed additive is subpackaged according to 1kg per package by adopting full-automatic quantification, and is packaged and sealed by adopting a company general feed additive packaging bag, so that the feed additive for improving the intestinal health of livestock and poultry and improving the anti-stress capability is obtained.
Example 3
The feed additive for improving the intestinal health and the anti-stress capability of livestock and poultry comprises the following components in percentage by mass: 1.5% of bacillus licheniformis, 3.5% of bacillus subtilis, 3.5% of bifidobacterium and 1.5% of enterococcus faecalis; 3.5% of honeysuckle extract, 3.5% of astragalus extract and 1.5% of liquorice extract; 1.0% of vitamin C; 1.0% of tea polyphenol; 3.0% of sodium humate; montmorillonite 70.0%; 1.0% of zymosan; 3.0% of gamma-aminobutyric acid is placed in a feed additive premixing machine, fully stirred for 180s, and after the uniform mixing is completed, the feed additive is subpackaged according to 1kg per package by adopting full-automatic quantification, and is packaged and sealed by adopting a common feed additive packaging bag of a company, so that the feed additive for improving the intestinal health of livestock and poultry and improving the anti-stress capability is obtained.
Example 4 feed additive for improving intestinal health and anti-stress ability of livestock and poultry
200 healthy broilers with the average weight of about 2.5kg are selected from cooperative broilers breeding enterprises of Zhejiang corporation, randomly divided into 4 groups, and sequentially comprise a normal-temperature group control group, experimental groups a, b and c, and 50 broilers in each group.
Test feed selection
Control group: the basic ration sold in the market is used as the conventional feed for daily feeding of the broilers.
Experimental group a: the control group conventional feed and the feed additive of the example 1 are added according to the proportion of 300.0g added in each 1.0T feed.
Experimental group b: the control group conventional feed and the feed additive of the example 2 are added according to the proportion of 300.0g added in each 1.0T feed.
Experimental group c: the control group conventional feed and the feed additive of the example 3 are added according to the proportion of 300.0g added in each 1.0T feed.
And (3) carrying out disinfection and sanitation management on the culture environment during the experiment, wherein the experiment time is 30d, the daily weight gain and feed intake of each group of broilers are recorded every day, and the blood of the broilers in each experiment group is collected every 10d for carrying out content analysis on SOD enzyme activity, GSP-PX enzyme activity and MAD in the blood serum.
And performing correlation analysis on the experimental data by adopting the sps 20.0 data analysis software.
The test results are shown in the following tables 1 and 2:
TABLE 1 experiment on the growth performance of broiler chicken as feed additive for improving the intestinal health of livestock and poultry and improving the anti-stress ability
Figure DEST_PATH_IMAGE002AAAA
TABLE 2 feed additive for improving intestinal health of livestock and fowl and increasing anti-stress ability and contents of SOD enzyme activity, GSP-PX enzyme activity and MAD in blood serum of broiler chicken
Figure DEST_PATH_IMAGE004AA
The feed additive for improving the intestinal health of livestock and poultry and improving the anti-stress capability can be seen from the table 1 and the table 2, and the anti-stress agent can obviously improve the oxidation resistance of pigs, promote the growth, obviously improve the activities of SOD enzyme and GSP-PX enzyme in the blood serum of broilers, reduce the content of MAD, reduce the damage of stress to organisms and improve the breeding benefit. Meanwhile, the test indexes of the embodiment 2 are better than those of the embodiments 1 and 3.
Example 4 feeding experiment of weaned piglets with feed additive for improving intestinal health and anti-stress ability of livestock and poultry
200 healthy piglets with the average weight of about 7.5kg of the cooperative breeding enterprises of the near-Yi company are selected and randomly divided into 4 groups, namely a normal-temperature group control group, experimental groups a, b and c, and 50 piglets in each group.
Test feed selection
Control group: the commercial basic ration is used as a conventional feed for daily feeding of weaned piglets.
Experimental group a: the control group conventional feed and the feed additive of the example 1 are added according to the proportion of adding 400.0g of the feed per 1.0T.
Experimental group b: the control group conventional feed and the feed additive of the example 2 are added according to the proportion of adding 400.0g of the feed per 1.0T.
Experimental group c: the control group conventional feed and the feed additive of the example 3 are added according to the proportion of adding 400.0g of the feed per 1.0T.
And (4) well disinfecting and hygienically managing the culture environment during the experiment, wherein the test time is 30d, and daily gain, feed intake, diarrhea rate and death rate of each group are recorded every day.
And performing correlation analysis on the experimental data by adopting the sps 20.0 data analysis software.
The test results are shown in table 3 below:
TABLE 3 feed additive experiment for improving intestinal health and anti-stress ability of livestock and poultry for weaned pig growth performance
Item Control group Experimental group a Experimental group b Experimental group c
Initial age of seed kg 7.56 7.58 7.55 7.54
Daily gain g 300.90 368.49 392.45 388.65
End weight kg 16.59 18.63 19.24 19.20
Daily food intake g 568.90 600.60 612.6 605.10
The ratio of the materials to the meat% 1.89 1.62 1.56 1.55
The mortality rate is% 2.00 0 0 0
The diarrhea rate% 38.50 12.70 10.50 11.60
The test results show that the feed additive for improving the intestinal health of livestock and poultry and improving the anti-stress capability improves the growth rate and the daily gain of piglets, reduces the feed conversion ratio, improves the feed conversion rate, improves the autoimmune capability of piglets and reduces the death rate compared with the conventional feed control group, and the feed additive for improving the intestinal health of livestock and poultry and improving the anti-stress capability has obvious effects on improving the animal health, improving the immunity, improving the growth performance, improving the anti-stress capability and the like.

Claims (2)

1. The feed additive for improving intestinal health and anti-stress capability of livestock and poultry is characterized by comprising the following components in percentage by mass:
1.0-2.0% of bacillus licheniformis, 3.0-4.0% of bacillus subtilis, 2.0-3.0% of bifidobacterium and 1.0-2.0% of enterococcus faecalis; 3.0 to 4.0 percent of honeysuckle extract, 3.0 to 4.0 percent of astragalus extract and 1.0 to 2.0 percent of liquorice extract; 0.5 to 1.0 percent of vitamin C; 0.5 to 1.0 percent of tea polyphenol; 2.0 to 4.0 percent of sodium humate; 65.0 to 70.0 percent of montmorillonite; 1.0 to 2.0 percent of zymosan; 2.0 to 4.0 percent of gamma-aminobutyric acid.
2. The feed additive for improving the intestinal health and the anti-stress capability of livestock and poultry according to claim 1, wherein the montmorillonite is nano montmorillonite with the particle size of 325 meshes.
CN202010347855.7A 2020-04-28 2020-04-28 Feed additive for improving intestinal health and anti-stress capability of livestock and poultry Withdrawn CN113558138A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114041535A (en) * 2021-11-24 2022-02-15 石河子大学 Microbial preparation for improving intestinal environment of ruminants such as cattle and sheep and preparation method thereof
CN114041536A (en) * 2021-11-24 2022-02-15 石河子大学 Composite Chinese herbal medicine microecological preparation and preparation method thereof
CN115176871A (en) * 2022-06-29 2022-10-14 瑞普高科(天津)生物技术有限公司 Anti-stress antioxidant feed additive and preparation method and application thereof
CN115211492A (en) * 2022-06-07 2022-10-21 圣道生物技术(山东)有限公司 Stress relieving medicine for resisting stress and promoting growth of livestock and poultry and preparation method and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109699837A (en) * 2017-10-25 2019-05-03 江西六合生物科技有限公司 A kind of feed addictive that child care pig intestinal health can be improved and promotes the growth of child care pig
CN109997958A (en) * 2018-01-04 2019-07-12 山东润牧生物科技有限公司 A kind of improvement animal and bird intestines are healthy, improve anti-stress ability feed addictive

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109699837A (en) * 2017-10-25 2019-05-03 江西六合生物科技有限公司 A kind of feed addictive that child care pig intestinal health can be improved and promotes the growth of child care pig
CN109997958A (en) * 2018-01-04 2019-07-12 山东润牧生物科技有限公司 A kind of improvement animal and bird intestines are healthy, improve anti-stress ability feed addictive

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114041535A (en) * 2021-11-24 2022-02-15 石河子大学 Microbial preparation for improving intestinal environment of ruminants such as cattle and sheep and preparation method thereof
CN114041536A (en) * 2021-11-24 2022-02-15 石河子大学 Composite Chinese herbal medicine microecological preparation and preparation method thereof
CN115211492A (en) * 2022-06-07 2022-10-21 圣道生物技术(山东)有限公司 Stress relieving medicine for resisting stress and promoting growth of livestock and poultry and preparation method and application thereof
CN115211492B (en) * 2022-06-07 2024-04-19 圣道生物技术(山东)有限公司 Stress-resistant growth-promoting stress-relieving medicine for livestock and poultry, and preparation method and application thereof
CN115176871A (en) * 2022-06-29 2022-10-14 瑞普高科(天津)生物技术有限公司 Anti-stress antioxidant feed additive and preparation method and application thereof

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