CN115176871A - Anti-stress antioxidant feed additive and preparation method and application thereof - Google Patents
Anti-stress antioxidant feed additive and preparation method and application thereof Download PDFInfo
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- CN115176871A CN115176871A CN202210764951.0A CN202210764951A CN115176871A CN 115176871 A CN115176871 A CN 115176871A CN 202210764951 A CN202210764951 A CN 202210764951A CN 115176871 A CN115176871 A CN 115176871A
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- Prior art keywords
- vitamin
- lactic acid
- acid bacteria
- feed additive
- stress
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- 230000002180 anti-stress Effects 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 230000003078 antioxidant effect Effects 0.000 title claims description 10
- 239000003963 antioxidant agent Substances 0.000 title claims description 9
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 96
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 56
- 241000894006 Bacteria Species 0.000 claims abstract description 53
- 239000004310 lactic acid Substances 0.000 claims abstract description 53
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 48
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 48
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
- A23K20/126—Lactones
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/41—Pediococcus
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Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Birds (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Botany (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Inorganic Chemistry (AREA)
- Fodder In General (AREA)
Abstract
The invention relates to an anti-stress and anti-oxidation feed additive and a preparation method and application thereof. The feed additive comprises the components of vitamin C, acanthopanax senticosus extract, taurine, lactic acid bacteria and diluent, and the vitamin C and the lactic acid bacteria are coated by adopting a fluidized bed coating technology. The feed additive is good in stability and storage-resistant, can rapidly supplement vitamin C, taurine and lactic acid bacteria for animals, relieve stress caused by various factors and reduce production performance caused by stress, can directly and indirectly act on free radicals in vivo, reduces damage of the free radicals to organism cells, improves oxidation resistance of organisms, has the effect of regulating intestinal flora, and integrally improves animal health level.
Description
Technical Field
The invention belongs to the technical field of animal nutrition, and particularly relates to an anti-stress and anti-oxidation feed additive, and a preparation method and application thereof.
Background
In modern intensive and large-scale breeding production, the production performance of animals directly relates to production benefits, pathogenic microorganisms directly cause the animals to suffer from diseases and die, the production capacity is reduced, stress also commonly occurs in the breeding process, and the stress is not easy to be perceived, find a stress source and be easily ignored by breeding technicians. Animal stress refers to the condition that after an animal encounters a stressor, the animal is firstly identified and stimulated by the central nervous system, and then a series of biological reactions are initiated by tissues to defend, namely animal stress reactions which comprise behavioral reactions, autonomic nervous system reactions, neuroendocrine system reactions and immune system reactions.
Under the modern large-scale production condition, the behavior regulation function of animals almost loses effect, which is a reason for the stress widely existing in the current intensive production, after the behavior reaction fails, a series of reactions such as nerve, metabolism, immunity and the like can appear next, the reactions are usually not easy to be perceived, no obvious symptoms exist, but the effects such as tension, endocrine dyscrasia, increase of oxidation free radicals, abnormal gastrointestinal tract function and the like are caused on the organism. In addition, animal stress has specificity, and specific biological responses can be generated aiming at different stressors, different animals and different living environments. Therefore, in the breeding process, the stress of the animals is difficult to prevent and occurs more or less, and the difference of understanding, importance and prevention of the stress of the breeding technicians can cause the animals to be affected by the stress. Epidemic diseases are easy to prevent, but stress is difficult to prevent and can cause great loss, so the stress problem needs to be solved urgently, the intangible loss is reduced to the minimum, and the animal welfare can be improved.
The heat stress is most common on the poultry breeding site, particularly in summer, the high-temperature and high-humidity weather in the southern area of China lasts for a long time, if the feeding management is not in place, the ventilation and cooling effects in the breeding house are poor, the heat stress can be directly caused, and great loss is caused for poultry breeding.
Most common anti-stress and anti-oxidation products are unstable, and are easily influenced by environmental factors such as high temperature, high humidity and the like during storage, so that the content is reduced, and the components lose efficacy; after water is dissolved, the water is affected by water quality, such as metal ion content, microorganisms and the like, so that the water rapidly loses efficacy in an aqueous solution. In order to solve the problems, a product which is convenient to directly use on a culture site and has a good anti-stress effect is needed.
Disclosure of Invention
The invention aims to solve the problems in the prior art and provides an anti-stress and anti-oxidation feed additive and a preparation method and application thereof.
In a first aspect of the invention, an anti-stress and anti-oxidation feed additive is provided, which comprises vitamin C, acanthopanax root extract, taurine, lactic acid bacteria and a diluent; the anti-stress and anti-oxidation feed additive comprises the following raw material components in parts by weight: 300-700 parts of vitamin C, 1-9 parts of acanthopanax extract, 70-150 parts of taurine, 10-20 parts of lactic acid bacteria and 1000 parts of diluent.
Preferably, the anti-stress and anti-oxidation feed additive comprises the following raw material components in parts by weight: 400-600 parts of vitamin C, 3-8 parts of acanthopanax root extract, 80-130 parts of taurine, 11-19 parts of lactic acid bacteria and 1000 parts of diluent.
More preferably, the functional feed additive product for preventing and controlling poultry bacterial enteritis comprises the following raw material components in parts by weight: 450-550 parts of vitamin C, 4-7 parts of acanthopanax extract, 90-120 parts of taurine, 12-18 parts of lactic acid bacteria and 1000 parts of diluent.
The lactobacillus is lactobacillus plantarum and pediococcus acidilactici, and the ratio of the lactobacillus plantarum to the pediococcus acidilactici is 1.
The vitamin C and lactobacillus are coated vitamin C-lactobacillus mixture, have white fine particles in appearance, high stability, storage stability, no influence from environmental factors, and easy dissolution in water, the vitamin C content is not less than 99.0%, and the lactobacillus content is not less than 1.0 × 10 8 CFU/g, and water content less than or equal to 0.5 percent.
The wall material of the coated vitamin C-lactic acid bacteria is one or more of hydroxypropyl methylcellulose, polyethylene glycol, cellulose acetate phthalate, ethyl cellulose and hydroxypropyl beta-cyclodextrin, and preferably the wall material is selected from the group consisting of polyethylene glycol and hydroxypropyl beta-cyclodextrin. The coating wall material can be utilized to form a layer of protective film on the surface of material particles by using the viscosity of the coating wall material, so that the coating effect can be achieved, and the coating can be quickly dissolved when being dissolved in water.
The diluent is one or more of glucose, soluble starch, lactose, sodium sulfate and sodium chloride, and preferably the diluent is glucose.
The invention provides a preparation method of the anti-stress and anti-oxidation feed additive, which comprises the following specific steps:
(1) Diluting and dissolving the wall material with purified water to prepare 1-7% wall material solution.
(2) Mixing vitamin C raw powder and lactobacillus powder uniformly to obtain soft material, and granulating with a swing granulator to obtain granules with diameter of 0.2-0.5mm.
(3) And putting the vitamin C-lactic acid bacteria particles into an FLP200 type fluidized bed system, introducing the wall material solution into the fluidized bed by using a peristaltic pump, coating, drying, detecting the content and the moisture, and obtaining the coated vitamin C-lactic acid bacteria after the product is qualified.
(4) Weighing the raw materials according to the formula amount, and diluting the acanthopanax senticosus extract with diluent for 4-5 times in equal amount to obtain a mixture A. And (3) fully mixing taurine with the mixture A to obtain a mixture B. And thoroughly mixing the mixture B with the rest of the diluent to obtain a mixture C. And (4) fully and uniformly mixing the mixture C and the coated vitamin C-lactic acid bacteria obtained in the step (3) to obtain the product of the invention.
Preferably, in the step (3), the coating temperature is 30-50 ℃, the drying temperature is 40-60 ℃ and the drying time is 2-4 hours.
Preferably, the amount of the wall material in the step (3) is 1-5% of the total amount of the vitamins and the probiotic mixed powder.
In a third aspect of the invention, the application of the anti-stress and anti-oxidation feed additive is provided, the anti-stress and anti-oxidation feed additive is mainly used for relieving stress caused by various factors and production performance reduction caused by stress, can directly and indirectly act on in vivo free radicals, reduces the damage of the free radicals to organism cells, improves the oxidation resistance of an organism, has the effect of regulating intestinal flora, and integrally improves the health level of animals.
The anti-stress and anti-oxidation feed additive is preferably used for livestock and poultry.
The anti-stress and anti-oxidation feed additive provided by the invention has good water solubility, is convenient to use in a breeding site, and is suitable for a drinking water system of intensive breeding.
The use method of the anti-stress and anti-oxidation feed additive provided by the invention specifically comprises the steps that the product can be used as drinking water, 50-100g of the product is added into each ton of drinking water, and the drinking water is completely drunk within 4-6 hours; when the ventilation and cooling effects of the breeding house are not good in high-temperature and high-humidity weather or the ventilation and cooling effects of the breeding house are not good, 100-200g of drinking water per ton is added into the drinking water, and the drinking water is drunk within 4-6 hours.
Has the advantages that:
1. the invention provides an anti-stress and anti-oxidation feed additive, which can rapidly supplement vitamin C, taurine and lactic acid bacteria for animals, relieve stress caused by various factors and production performance reduction caused by stress; the feed additive is used in stages of brooding, immunization, group transfer and expansion, epidemic disease recovery, material change, slaughter, summer hot weather and the like which are easy to cause stress response, can improve the anti-stress capability and disease resistance of animal organisms, maintain the self-immunity capability of the organisms, and prevent the problems of listlessness, decreased feed intake, increased brooding, increased morbidity, increased mortality and the like which are easy to occur in the stress stage; directly and indirectly act on in vivo free radicals, reduce the damage of the free radicals to organism cells, improve the oxidation resistance of the organism and improve the integral health level. Can be used as an additive product for preventing and treating various adverse reactions caused by the stress of poultry.
2. The coated vitamin C-lactic acid bacteria used in the invention have the characteristics of storage resistance, good stability, difficult influence by environmental factors and easy water dissolution when being stored in a solid form, and are convenient for being used in poultry breeding sites.
3. The lactic acid bacteria can be used for producing gamma-aminobutyric acid through fermentation, have the effects of calming and reducing animal stress, and can supplement intestinal lactic acid bacteria, regulate the balance of intestinal flora and improve immunity.
4. The acanthopanax extract mainly contains eleutheroside B and eleutheroside E as active ingredients, and also contains Superoxide dismutase (SOD) compound, wherein the SOD has good antioxidant function, can enhance organism immunity and resist free radical attack, has antioxidant effect, and can protect vitamin C after being dissolved in water due to the fact that the reducibility of the SOD is stronger than that of the vitamin C, and damage of environmental factors to the vitamin C is reduced.
Detailed Description
The present invention will be further described with reference to the following examples, but the embodiments of the present invention are not limited thereto, and the experimental methods used in the following examples are all conventional methods unless otherwise specified.
Example 1
(1) And diluting and dissolving hydroxypropyl beta-cyclodextrin with purified water to prepare a 2% wall material solution.
(2) Mixing 300g of vitamin C raw powder and 10g of lactobacillus powder (Lactobacillus plantarum: pediococcus acidilactici is 1.
(3) Putting the vitamin C-lactobacillus granules into an FLP200 type fluidized bed system (bottom spraying), introducing the wall material solution into a fluidized bed by using a peristaltic pump, coating at the temperature of 30 ℃, drying at the temperature of 40 ℃ for 4 hours, detecting the content and the moisture, and obtaining the coated vitamin C-lactobacillus after the product is qualified.
(4) 1g of acanthopanax senticosus extract was diluted 5 times with the same amount of glucose to obtain a mixture a. 70g of coated taurine was mixed well with mixture A to give mixture B. And thoroughly mixing the mixture B with the rest glucose to obtain a mixture C. And (4) fully and uniformly mixing the mixture C and the coated vitamin C-lactic acid bacteria obtained in the step (3) to obtain 1000g of the product.
Example 2
(1) Diluting and dissolving polyethylene glycol with purified water to prepare a 3% wall material solution.
(2) Mixing 400g of vitamin C raw powder and 11g of lactobacillus powder (Lactobacillus plantarum: pediococcus acidilactici is 1.
(3) Putting the vitamin C-lactic acid bacteria particles into an FLP200 type fluidized bed system (bottom spraying), introducing the wall material solution into the fluidized bed by using a peristaltic pump, coating at the temperature of 35 ℃, drying at the temperature of 45 ℃ for 3 hours, detecting the content and the moisture, and obtaining the coated vitamin C-lactic acid bacteria after the coated vitamin C-lactic acid bacteria are qualified.
(4) Diluting 3g of Acanthopanax senticosus extract with glucose for 5 times to obtain mixture A. 80g of taurine was mixed well with the mixture A to obtain a mixture B. And thoroughly mixing the mixture B with the residual glucose to obtain a mixture C. And (4) fully and uniformly mixing the mixture C and the coated vitamin C-lactic acid bacteria obtained in the step (3) to obtain 1000g of the product.
Example 3
(1) And diluting and dissolving hydroxypropyl beta-cyclodextrin with purified water to prepare a 4% wall material solution.
(2) Uniformly mixing 500g of vitamin C raw powder and 16g of lactobacillus powder (Lactobacillus plantarum: pediococcus acidilactici is 1.
(3) Putting the vitamin C-lactic acid bacteria particles into an FLP200 type fluidized bed system (bottom spraying), introducing the wall material solution into the fluidized bed by using a peristaltic pump, coating at the temperature of 40 ℃, drying at the temperature of 50 ℃ for 2.5 hours, detecting the content and the moisture, and obtaining the coated vitamin C-lactic acid bacteria after the product is qualified.
(4) 5g of the acanthopanax senticosus extract was diluted 4 times with the same amount of glucose to obtain a mixture A. 110g of taurine was mixed well with mixture A to give mixture B. And thoroughly mixing the mixture B with the rest glucose to obtain a mixture C. And (4) fully and uniformly mixing the mixture C and the coated vitamin C-lactic acid bacteria obtained in the step (3) to obtain 1000g of the product.
Example 4
(1) And diluting and dissolving hydroxypropyl beta-cyclodextrin with purified water to prepare a 5% wall material solution.
(2) Mixing 600g of vitamin C raw powder and 19g of lactobacillus powder (Lactobacillus plantarum: pediococcus acidilactici is 1.
(3) Putting the vitamin C-lactic acid bacteria particles into an FLP200 type fluidized bed system (bottom spraying), introducing the wall material solution into the fluidized bed by using a peristaltic pump, coating at the temperature of 45 ℃, drying at the temperature of 55 ℃ for 3 hours, detecting the content and the moisture, and obtaining the coated vitamin C-lactic acid bacteria after the coated vitamin C-lactic acid bacteria are qualified.
(4) 7g of the acanthopanax senticosus extract was diluted 4 times with the same amount of glucose to obtain a mixture A. 130g of taurine was mixed well with mixture A to give mixture B. And thoroughly mixing the mixture B with the residual glucose to obtain a mixture C. And (4) fully and uniformly mixing the mixture C and the coated vitamin C obtained in the step (3) to obtain 1000g of the product.
Example 5
(1) Diluting and dissolving polyethylene glycol with purified water to prepare 6% wall material solution.
(2) Mixing 700g of vitamin C raw powder and 20g of lactobacillus powder (Lactobacillus plantarum: pediococcus acidilactici is 1.
(3) Putting the vitamin C-lactobacillus particles into an FLP200 type fluidized bed system (bottom spraying), introducing the wall material solution into the fluidized bed by using a peristaltic pump, coating at the temperature of 50 ℃, drying at the temperature of 60 ℃ for 2 hours, detecting the content and the moisture, and obtaining the coated vitamin C-lactobacillus after the product is qualified.
(4) 9g of acanthopanax senticosus extract was diluted 4 times with an equal amount of glucose to obtain a mixture a. 150g of taurine was mixed well with mixture A to give mixture B. And thoroughly mixing the mixture B with the residual glucose to obtain a mixture C. And (4) fully and uniformly mixing the mixture C and the coated vitamin C-lactic acid bacteria obtained in the step (3) to obtain 1000g of the product.
Comparative example 1 example 3 product using gamma aminobutyric acid instead of lactic acid bacteria
(1) And diluting and dissolving hydroxypropyl beta-cyclodextrin with purified water to prepare a 4% wall material solution.
(2) 500g of vitamin C raw powder is prepared into a soft material, and the soft material is granulated by a swinging granulator, wherein the diameter of the granules is 0.2-0.5mm.
(3) Putting the vitamin C particles into an FLP200 type fluidized bed system (bottom spraying), introducing the wall material solution into the fluidized bed by using a peristaltic pump, coating at the temperature of 40 ℃, drying at the temperature of 50 ℃ for 2.5 hours, detecting the content and the moisture, and obtaining the coated vitamin C after the vitamin C particles are qualified.
(4) 5g of the acanthopanax senticosus extract was diluted 4 times with the same amount of glucose to obtain a mixture A. 110g of taurine was mixed well with the mixture A to obtain a mixture B. The mixture B was thoroughly mixed with the remaining glucose and 50g of gamma aminobutyric acid to obtain a mixture C. And (4) fully and uniformly mixing the mixture C and the coated vitamin C obtained in the step (3) to obtain 1000g of the product.
Comparative example 2 example 3 product without Acanthopanax senticosus extract
(1) And diluting and dissolving hydroxypropyl beta-cyclodextrin with purified water to prepare a 4% wall material solution.
(2) Mixing 500g of vitamin C raw powder and 16g of lactobacillus powder uniformly to prepare a soft material, and granulating by a swing granulator, wherein the particle diameter is 0.2-0.5mm.
(3) Putting the vitamin C-lactic acid bacteria particles into an FLP200 type fluidized bed system (bottom spraying), introducing the wall material solution into the fluidized bed by using a peristaltic pump, coating at the temperature of 40 ℃, drying at the temperature of 50 ℃ for 2.5 hours, detecting the content and the moisture, and obtaining the coated vitamin C-lactic acid bacteria after the product is qualified.
(4) 110g of taurine was thoroughly mixed with the remaining glucose to obtain a mixture. And (4) fully and uniformly mixing the mixture and the coated vitamin C-lactic acid bacteria obtained in the step (3) to obtain 1000g of the product.
Comparative example 3 product without vitamin-probiotic coating
Accurately weighing the following raw materials in proportion: 500g of vitamin C, 110g of taurine, 5g of acanthopanax extract, 16g of lactic acid bacteria and 369g of glucose. Diluting radix Acanthopanacis Senticosi extract with glucose for 4 times to obtain mixture A. And (3) fully mixing taurine with the mixture A to obtain a mixture B. And thoroughly mixing the mixture B with the residual glucose to obtain a mixture C. And (5) fully and uniformly mixing the mixture C, the vitamin C and the lactic acid bacteria to obtain 1000g of the product.
Comparative example 4
Comparative example 4 the product was according to chinese patent (CN 104839514A): a feed additive for improving the stress resistance of weaned pigs and a preparation method and an application thereof are prepared in the embodiment 1.
EXAMPLE 6 Oxidation resistance and stress resistance test of the product of the invention
Experimental products: examples 1, 2, 3, 4 and 5 of the present invention
Experimental animals: broiler chicken, 200
The experimental steps are as follows:
(1) Broiler chicks of 1 day old, without any immunization, were raised to 10 days old in a suitable environment.
(2) At 10 days of age, all chickens were weighed and recorded, and apparently heavy and apparently light chickens were culled, leaving 180 chicks in close proximity at random, and divided into 6 groups of 30 chickens, each group corresponding to example 1, example 2, example 3, example 4, example 5, and a blank control group.
(3) The products of examples 1-5 were added to drinking water from 11-30 days of age, at a dosage of 100g per ton of drinking water per day for the groups of examples 1-5; the blank control group was not supplemented with the same type of additives. The temperature in the chicken house was raised by 5 ℃ at 10 to 18 o' clock each day while the noise was let off for 10 minutes per hour.
(4) Weighing the weight of each group of chickens once every 5 days from the age of 11 days, and calculating the average weight of each group of chickens; blood was collected from all chickens at 30 days of age via vein, and total antioxidant capacity (T-AOC), superoxide dismutase (SOD) and Malondialdehyde (MDA) of each group of chickens were examined.
The experimental results are as follows:
TABLE 1 average weight of chickens in each group
TABLE 2 Total antioxidant capacity of 30-day-old chickens
TABLE 3 superoxide dismutase content in 30-day-old chickens
TABLE 4 malondialdehyde content in 30-day-old groups of chickens
The experimental conclusion is that:
the average weight of the chickens in the groups of examples 1-5 is larger than that of the blank control group by comparing the average weight of the chickens in each group, which shows that the additive can resist the influence of stress on the production performance of the chicken flocks. Comparing the total oxidation resistance of all groups of chickens, the contents of superoxide dismutase and malonaldehyde, the results show that the total oxidation resistance and superoxide dismutase of the chickens of the groups 1-5 are greater than those of the blank control group, and the content of malonaldehyde is less than that of the blank control group, which shows that the additive can improve the oxidation resistance of the chickens and reduce the content of oxidative free radicals.
The average body weight, total antioxidant capacity and malondialdehyde content of the chickens in examples 1-5 were compared to find that the effect of examples 3, 4 and 5 was better than that of examples 1 and 2, indicating that the higher the content of vitamin C, taurine, acanthopanax senticosus extract and lactic acid bacteria, the stronger the anti-stress antioxidant capacity, and considering the input and output, the lower the cost performance of examples 4 and 5 is compared to example 3, indicating that example 3 is the optimal formulation.
Example 7PTIO free radical scavenging assay
Experimental products: inventive example 3 and comparative example 4
The experimental steps are as follows:
(1) Dissolving 3mg of PTIO solid in 20ml of distilled water, performing ultrasonic treatment for 5min, and sufficiently shaking to ensure that the upper part and the lower part are uniform to obtain a PTIO test solution.
(2) 0.1g of inventive example 3 and comparative example 4 were dissolved in 100ml of distilled water, and the mixture was subjected to ultrasonic treatment for 5 minutes and sufficiently shaken to make the upper and lower portions uniform, thereby obtaining the inventive test solution of inventive example 3 and the comparative example 4.
(3) Carrying out a preliminary experiment, taking 800 mu l of PTIO test solution, adding a small amount of the test solution of the invention in the embodiment 3, gradually adding a small amount of the test solution in the adding process, mixing the test solution while adding the test solution, observing the fading condition of the solution, and recording the sample adding amount V of the sample when the color of the solution is basically faded 1 .3 replicates were performed.
(4) Comparative example 4 the test solution was subjected to the same operation as in step (3), and the amount of sample V was recorded 2 .3 replicates were performed.
The experimental results are as follows:
TABLE 5 comparison of test fluid consumption volumes
| Repetition of 1 | Repetition 2 | Repetition of 3 | Average out | |
| V 1 (μl) | 84 | 87 | 85 | 85.3 |
| V 2 (μl) | 148 | 151 | 147 | 148.7 |
And (4) experimental conclusion:
PTIO is a stable water-soluble oxidizing free radical and can quantitatively reflect the capability of a test substance for eliminating the PTIO, so that the lower the consumption of the test substance is under the condition of the same PTIO content, the stronger the capability of the test substance for eliminating the oxidizing free radical is. According to the experimental results, the ability of example 3 of the present invention to eliminate oxidative radicals is significantly stronger than that of comparative example 4.
Example 8 palatability and production Performance testing
Experimental product: inventive example 3 and comparative example 1
Experimental animals: 60-day-old nursery pig with 60 heads
The experimental steps are as follows:
(1) The nursery pigs with uniform body weight were randomly divided into 3 groups, which were blank control groups, example 3 groups, comparative example 1 groups, and 20 pigs each.
(2) The example 3 group and the comparative example 1 group were added with the product of example 3 and the product of comparative example 1 at an addition amount of 100g/t of feed, and the blank control group was not added with any additive product.
(3) The food intake is counted every 10 days, the average food intake is calculated, the weight is weighed every 10 days, and the average weight is calculated.
The experimental results are as follows:
TABLE 6 comparison of average feed intake of pigs in each group
| 61-70 days old (kg) | 71-80 days old (kg) | Age 81-90 days (kg) | |
| EXAMPLE 3 group | 1.26 | 1.48 | 1.70 |
| Comparative example 1 group | 1.10 | 1.29 | 1.48 |
| Blank control group | 1.14 | 1.34 | 1.51 |
Comparing the average feed intake of the pigs in each group, the average feed intake of the pigs in example 3 is significantly higher than that of the pigs in comparative example 1 and the pigs in the blank control group, and the average feed intake of the pigs in comparative example 1 is slightly lower than that of the pigs in the blank control group.
TABLE 7 comparison of average weight of pigs in each group
| 60 days old (kg) | Age 70 days (kg) | 80 days old (kg) | 90 days old (kg) | |
| EXAMPLE 3 group | 21.37 | 27.33 | 33.86 | 40.88 |
| Comparative example 1 group | 18.38 | 24.85 | 30.69 | 37.59 |
| Blank control group | 19.12 | 25.52 | 31.59 | 38.07 |
Comparing the average weight of the pigs in each group, the average weight of the pigs in the example 3 group is significantly higher than that of the pigs in the comparative example 1 group and the blank control group, and the average weight of the pigs in the comparative example 1 group is slightly lower than that of the blank control group.
And (4) experimental conclusion:
the feed intake of the pigs in the group of example 3 was the highest, and the feed intake of the pigs in the group of comparative example 1 was slightly lower than that of the blank control group, which indicates that the palatability of the pigs is affected by the product of comparative example 1, and the reason for the analysis should be related to the slightly bitter taste of gamma aminobutyric acid, which affects the feed intake of the pigs. The weight of the pigs in the group in example 3 is obviously higher than that of the pigs in the group in comparative example 1 and the blank control group, which shows that the product in example 3 has obvious growth promotion effect, and the analysis reason is related to the fact that lactobacillus regulates the intestinal flora of the pigs and promotes the digestion and absorption of feed. In conclusion, the lactobacillus capable of producing gamma aminobutyric acid by fermentation is adopted in the embodiment 3, the feed intake of the pigs is not influenced, and the effect of improving the production performance of the pigs is achieved.
Example 9 high temperature resistance test of the product
Experimental product: inventive example 3, comparative example 2, and comparative example 3
The experimental steps are as follows:
(1) 2 plates were taken and 5g of each of example 3 of the present invention, comparative example 2 and comparative example 3 was weighed into a plate and placed in an oven at 60 ℃ for 48 hours.
(2) The change in appearance was compared between example 3 of the present invention and comparative examples 2 and 3.
(3) The contents of vitamin C and taurine in example 3 and comparative example 2 and comparative example 3 of the present invention were measured.
The experimental results are as follows:
(1) The change in the appearance shape was observed after a high temperature of 60 ℃ for 48 hours:
the results show that after 48 hours at 60 c, the sample of comparative example 2 showed blocking, the sample of comparative example 3 showed blocking and slight discoloration, and there was no visible change to the naked eye in example 3.
(2) The content of vitamin C and probiotics changes after the high temperature of 60 ℃ is 48 hours
TABLE 8 change in vitamin C and taurine content after 48 hours at 60 deg.C
After the high temperature of 60 ℃ is carried out for 48 hours, the vitamin content is reduced by 0.33 percent and the lactic acid bacteria content is reduced by 14.75 percent in the example 3; comparative example 2 the vitamin C content is reduced by 3.75 percent, the lactic acid bacteria content is reduced by 16.76 percent; comparative example 3 the vitamin C content was reduced by 8.44% and the lactic acid bacteria content was reduced by 63.08%.
The experimental conclusion is that:
the results of appearance property change, vitamin C content change and lactic acid bacteria content change of comparative example 2 and comparative example 3 after the high temperature experiment were comprehensively compared, which indicates that example 3 is more stable, less susceptible to environmental factors, and more storage-resistant.
EXAMPLE 10 stability testing of the product in aqueous solution
Experimental products: inventive example 3, comparative example 2, and comparative example 3
The experimental steps are as follows:
(1) 1g (to the nearest 0.0001 g) of each of example 3 of the present invention, comparative example 2 and comparative example 3 was weighed, and placed in 2 beakers, 200ml of purified water was added to each beaker, and the beaker was allowed to stand at normal temperature for 12 hours.
(2) The vitamin C and lactic acid bacteria content was measured every 2 hours for 1 time.
The experimental results are as follows:
TABLE 9 vitamin C content Change
The vitamin C content of the aqueous solution in example 3 is slightly reduced with the time being prolonged, and is reduced by 1.12% in 12 hours; the vitamin C content of the aqueous solution of the comparative example 2 is obviously reduced by 7.49 percent in 12 hours, and the vitamin C content of the aqueous solution of the comparative example 3 is obviously reduced by 13.12 percent in 12 hours.
TABLE 10 lactic acid bacteria content variation
| Example 3 (CFU/ml) | COMPARATIVE EXAMPLE 2 (CFU/ml) | COMPARATIVE EXAMPLE 3 (CFU/ml) | |
| 0 hour | 5.02×10 5 | 5.01×10 5 | 5.02×10 5 |
| 2 hours (h) | 5.01×10 5 | 5.00×10 5 | 4.95×10 5 |
| 4 hours | 5.00×10 5 | 4.97×10 5 | 4.81×10 5 |
| 6 hours | 4.98×10 5 | 4.93×10 5 | 4.68×10 5 |
| 8 hours | 4.92×10 5 | 4.85×10 5 | 4.55×10 5 |
| 10 hours | 4.93×10 5 | 4.87×10 5 | 4.39×10 5 |
| 12 hours | 4.91×10 5 | 4.84×10 5 | 4.11×10 5 |
With the time, the content of the lactic acid bacteria in the water solution in the example 3 is slightly reduced, and is reduced by 2.19 percent in 12 hours; the content of the lactobacillus in the aqueous solution of the comparative example 2 is slightly reduced by 3.39 percent in 12 hours, the content of the lactobacillus in the aqueous solution of the comparative example 3 is obviously reduced by 18.13 percent in 12 hours.
The experimental conclusion is that:
after the comprehensive comparison aqueous solution standing experiment, the vitamin C content of the aqueous solution in the example 3 is relatively stable, and the vitamin C content of the aqueous solutions in the comparative examples 2 and 3 is obviously reduced; the lactic acid bacteria content of the water solution of the example 3 and the water solution of the comparative example 2 are stable, the lactic acid bacteria content of the water solution of the example 3 is obviously reduced, and the fact that the vitamin C content and the lactic acid bacteria content of the water solution of the example 3 are stable is proved that the water solution is more suitable for being added into drinking water.
Claims (10)
1. An anti-stress and anti-oxidation feed additive is characterized by comprising vitamin C, an acanthopanax senticosus extract, taurine, lactic acid bacteria and a diluent.
2. The feed additive according to claim 1, wherein the feed additive comprises the following raw materials in parts by weight: 300-700 parts of vitamin C, 1-9 parts of acanthopanax extract, 70-150 parts of taurine, 10-20 parts of lactic acid bacteria and 1000 parts of diluent.
3. The feed additive as claimed in claim 2, wherein the feed additive comprises the following raw materials in parts by weight: 400-600 parts of vitamin C, 3-8 parts of acanthopanax extract, 80-130 parts of taurine, 11-19 parts of lactic acid bacteria and 1000 parts of diluent.
4. The feed additive according to any one of claims 1 to 3, wherein the lactic acid bacteria are Lactobacillus plantarum and Pediococcus acidilactici, and the ratio of Lactobacillus plantarum to Pediococcus acidilactici is 1; the vitamin C and the lactobacillus are coated vitamin C-lactobacillus.
5. The feed additive according to claim 4, wherein the coating wall material of the coated vitamin C-lactic acid bacteria is one or more of hydroxypropyl methylcellulose, polyethylene glycol, cellulose acetate phthalate, ethyl cellulose and hydroxypropyl beta-cyclodextrin.
6. The feed additive according to claim 5, wherein the coating wall material is one or both of polyethylene glycol and hydroxypropyl β -cyclodextrin.
7. The feed additive according to any one of claims 1 to 3, wherein the diluent is one or more of glucose, soluble starch, lactose, sodium sulfate and sodium chloride.
8. A method for preparing the anti-stress and anti-oxidation feed additive as defined in any one of claims 1-3, the preparation steps are as follows:
(1) Diluting and dissolving the coating wall material with purified water to prepare a 1-7% wall material solution;
(2) Mixing vitamin C raw powder and lactobacillus powder uniformly to obtain soft material, and granulating with a swing granulator to obtain granules with diameter of 0.2-0.5mm;
(3) Putting the vitamin C-lactic acid bacteria particles into an FLP200 type fluidized bed system, introducing the wall material solution into a fluidized bed by using a peristaltic pump, coating, drying, detecting the content and the moisture, and obtaining the coated vitamin C-lactic acid bacteria after the product is qualified;
(4) Weighing the raw materials according to the formula amount, diluting the acanthopanax senticosus extract with diluent for 4-5 times in equal amount to obtain a mixture A; fully mixing taurine with the mixture A to obtain a mixture B; fully mixing the mixture B with the rest of the diluent to obtain a mixture C; and (4) fully and uniformly mixing the mixture C and the coated vitamin C-lactic acid bacteria obtained in the step (3) to obtain the vitamin C-lactic acid bacteria.
9. The method according to claim 8, wherein the coating temperature in the step (3) is 30 to 50 ℃, the drying temperature is 40 to 60 ℃, and the drying time is 2 to 4 hours.
10. The use of an anti-stress and anti-oxidant feed additive as defined in any one of claims 1-3 for relieving stress in poultry and stress-induced deterioration of poultry productivity, maintaining body immunity, reducing damage of free radicals to body cells, and improving body antioxidant capacity.
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| CN110506843A (en) * | 2019-08-13 | 2019-11-29 | 安徽快康生物科技有限公司 | It is a kind of for building up health, improving the feed addictive and feed of anti-stress ability |
| CN111194849A (en) * | 2018-11-16 | 2020-05-26 | 广州每日膳道生物科技有限公司 | 0-degree sports solid beverage and preparation process thereof |
| CN113558138A (en) * | 2020-04-28 | 2021-10-29 | 浙江启润生物科技有限公司 | Feed additive for improving intestinal health and anti-stress capability of livestock and poultry |
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| CN105614002A (en) * | 2014-11-07 | 2016-06-01 | 北京大北农科技集团股份有限公司 | Oxidative stress resistant cow feed additive, premix, concentrated feed and compound feed |
| CN109418506A (en) * | 2017-08-23 | 2019-03-05 | 北京农博微生物科技有限公司 | A kind of stabilization coating technique of the feeding biological vitamin of enteric solubility |
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| CN109007344A (en) * | 2018-10-19 | 2018-12-18 | 河南牧业经济学院 | A kind of compound feed additive of anti-cow heat stress and preparation method thereof |
| CN109007343A (en) * | 2018-10-19 | 2018-12-18 | 河南牧业经济学院 | A kind of compound feed additive and preparation method thereof of anti-beef cattle transport stress |
| CN111194849A (en) * | 2018-11-16 | 2020-05-26 | 广州每日膳道生物科技有限公司 | 0-degree sports solid beverage and preparation process thereof |
| CN110506843A (en) * | 2019-08-13 | 2019-11-29 | 安徽快康生物科技有限公司 | It is a kind of for building up health, improving the feed addictive and feed of anti-stress ability |
| CN113558138A (en) * | 2020-04-28 | 2021-10-29 | 浙江启润生物科技有限公司 | Feed additive for improving intestinal health and anti-stress capability of livestock and poultry |
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