CN111011588A - Feed additive for preventing and treating cow mastitis and preparation method thereof - Google Patents

Feed additive for preventing and treating cow mastitis and preparation method thereof Download PDF

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CN111011588A
CN111011588A CN201911413824.0A CN201911413824A CN111011588A CN 111011588 A CN111011588 A CN 111011588A CN 201911413824 A CN201911413824 A CN 201911413824A CN 111011588 A CN111011588 A CN 111011588A
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equal
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feed additive
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胡红伟
李薇
雷变兰
闫凌鹏
麻啸涛
陈茹茹
任帅
党亚朋
杨京娥
权豪强
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Shanxi Dayu Bioengineering Co ltd
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Abstract

The invention relates to a feed additive for preventing and treating cow mastitis, which comprises the following components in parts by weight: 70-90 parts of fermented traditional Chinese medicine composition, 5-10 parts of sodium humate, 5-10 parts of saccharomyces cerevisiae cell walls, 3-5 parts of composite organic trace elements and 3-5 parts of composite vitamins. The components of the prescription of the invention are green and antibiotic-free raw materials, mainly fermented traditional Chinese medicines are used, and a plurality of beneficial components are matched, so that the somatic cells of the dairy cows can be obviously reduced and the milk quality can be improved.

Description

Feed additive for preventing and treating cow mastitis and preparation method thereof
Technical Field
The invention belongs to the technical field of novel feed additives, and particularly relates to a feed additive for preventing and treating cow mastitis and a preparation method thereof.
Background
Cow mastitis is a common disease of cows, and mastitis is an inflammatory disease caused by the stimulation of mammary tissue by pathogenic bacteria or physical, chemical and other factors. More than 150 pathogenic microorganisms causing mastitis infection of the dairy cattle are provided, the microbial infection is a main cause of a plurality of factors, and common pathogenic bacteria causing the mastitis include escherichia coli, staphylococcus aureus, streptococcus agalactiae, streptococcus dysgalactiae, streptococcus uberis, mycoplasma, pyogenes and the like. According to clinical manifestations, cow mastitis can be classified into clinical mastitis and invisible mastitis, and not only does cow mastitis cause a decrease in milk yield and quality, but also increases treatment costs. About 33% of 2.2 hundred million cows all over the world have mammary infection to different degrees, the economic loss caused by cow mastitis is up to $ 350 million each year, and the loss in China is up to 30 million yuan.
According to the traditional method for preventing and treating cow mastitis, antibiotics are mainly used for treatment, such as penicillin, amoxicillin, ceftiofur sodium and the like, with the use of the antibiotics, drug-resistant bacteria are continuously screened and domesticated, the larger the dosage of the antibiotics is, the more undesirable the treatment effect is, and meanwhile, the antibiotic residue may endanger the health of human beings.
The Chinese herbal medicine is natural plant, has the advantages of small toxic and side effects, difficult generation of drug resistance and the like, and has the effects of improving the immunity of the organism, improving the internal secretion, regulating the lactation function, resisting inflammation and the like on the prevention and treatment of the mastitis of the dairy cow.
The sodium humate is prepared from natural organic acid, can promote animal growth and development and increase milk yield of dairy cows, has the effects of stopping dysentery, stopping diarrhea, stopping bleeding, diminishing inflammation and the like, can effectively kill pathogenic bacteria and reduce the probability of infecting the pathogenic bacteria of the dairy cows. The cell wall of the saccharomyces cerevisiae improves the immunity of the dairy cows, and the compound organic trace elements and the compound vitamins provide necessary nutritional factors for the growth and development of the dairy cows, enhance the resistance and reduce the incidence rate of mastitis.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a feed additive for preventing and treating cow mastitis, which comprises the following raw materials in parts by weight: 70-90 parts of fermented traditional Chinese medicine composition, 5-10 parts of sodium humate, 5-10 parts of saccharomyces cerevisiae cell walls, 3-5 parts of composite organic trace elements and 3-5 parts of composite vitamins.
Preferably, the fermented Chinese herbal medicine composition is prepared from the following traditional Chinese medicines in parts by weight: 100-150 parts of dandelion, 100-150 parts of honeysuckle, 50-100 parts of houttuynia cordata, 30-80 parts of Chinese violet, 50-100 parts of common andrographis herb, 10-50 parts of purslane, 30-50 parts of uniflower swisscentaury root, 50-80 parts of akebia stem, 30-50 parts of beautiful sweetgum fruit, 30-50 parts of cowherb seed, 30-50 parts of angelica, 40-60 parts of codonopsis pilosula, 40-60 parts of astragalus membranaceus, 10-30 parts of scutellaria baicalensis, 30-60 parts of prepared rehmannia rhizome, 30-80 parts of white paeony root, 20-50 parts of ligusticum wallichii and 30-60 parts of liquorice.
In any of the above schemes, preferably, the fermented Chinese herbal medicine composition is composed of the following Chinese herbal medicines in parts by weight: the fermented traditional Chinese medicine composition is prepared from the following traditional Chinese medicines in parts by weight: 130 parts of dandelion, 140 parts of honeysuckle, 80 parts of houttuynia cordata, 50 parts of Chinese violet, 60 parts of common andrographis herb, 30 parts of purslane, 50 parts of uniflower swisscentaury root, 50 parts of akebia stem, 30 parts of beautiful sweetgum fruit, 40 parts of cowherb seed, 50 parts of szechuan lovage rhizome and 60 parts of liquorice.
In any of the above schemes, preferably, the composite organic trace element is composed of the following raw materials in parts by weight: 20-30 parts of zinc glycinate, 10-20 parts of manganese glycinate, 10-20 parts of copper glycinate and 40-60 parts of yeast selenium.
In any of the above schemes, the vitamin complex preferably comprises the following raw materials, by weight, 50-60 parts of vitamin E, 20-30 parts of vitamin A, and 20-30 parts of β -carotene.
In any scheme, the quality standard of the sodium humate is more than or equal to 70 percent of humic acid and less than or equal to 10 percent of water, the quality standard of cell walls of the saccharomyces cerevisiae is less than or equal to 35 percent of crude protein, more than or equal to 20 percent of mannan, more than or equal to 20 percent of β -glucan and less than or equal to 8 percent of water, the quality standard of the composite organic trace elements is that zinc glycinate is more than or equal to 22 percent of glycine, Zn is more than or equal to 21 percent of water and less than or equal to 10 percent of water, manganese glycinate is more than or equal to 10 percent of Mn, total glycine is more than or equal to 26 percent of water and less than or equal to 10 percent of copper glycinate, Cu is more than or equal to 22 percent of total glycine is more than or equal to 25 percent of water and less than or equal to 10 percent of yeast selenium is more than or equal to 2000mg/kg, and the.
The invention also provides a preparation method of the feed additive for preventing and treating cow mastitis, which comprises the following steps in sequence:
(1) weighing fermented traditional Chinese medicine composition, sodium humate, cell walls of saccharomyces cerevisiae, compound organic trace elements and compound vitamins according to parts;
(2) adding the raw materials into a mixer, starting the mixer to mix for 5 minutes to obtain the material, wherein the coefficient of variation CV of the mixing uniformity is less than or equal to 5 percent.
Preferably, the preparation method of the fermented traditional Chinese medicine composition comprises the following steps:
(1) pulverizing the above Chinese medicinal materials, and sieving with 60 mesh sieve;
(2) weighing the traditional Chinese medicine components according to the weight parts of the traditional Chinese medicines, adding the traditional Chinese medicine components into a mixer, premixing for 5 minutes, uniformly mixing trichoderma longibrachiatum liquid with water, adding the mixture into the mixer, and mixing with the traditional Chinese medicines;
(3) feeding the mixed materials into a fermentation chamber, controlling the temperature of the fermentation chamber to be 25-30 ℃ and the humidity to be 40-60%, turning over every 6 hours, and fermenting for 5 d;
(4) and drying the fermented material by adopting a boiling drying method, controlling the drying temperature to be 65-80 ℃, and drying to obtain the fermented traditional Chinese medicine composition.
In any of the above embodiments, preferably, the preparation of trichoderma longibrachiatum liquid comprises the following steps:
(1) picking a ring of trichoderma longibrachiatum inclined plane seeds, inoculating the seeds into an MEA liquid culture medium, performing shake culture at 25 ℃ at 200r/min for 3d, then inoculating a bacterial liquid into an MEA solid inclined plane culture medium, and performing standing culture for 5-7 d to grow light green spores on the inclined plane;
(2) washing the inclined plane of the trichoderma longibrachiatum by using 2% of tween 80 to obtain a suspension, putting the suspension into a sterile triangular flask with glass beads, putting the triangular flask into a 200r/min shaking table for oscillation, and scattering spores to obtain a spore suspension;
(3) inoculating the spore suspension obtained in the step (2) into a seeding tank according to the inoculation amount of 5%, wherein the culture temperature is 25-28 ℃, the rotation speed of the seeding tank is 150-200 r/min, the ventilation ratio is 1: 0.7-1: 1, and the culture time is 48-72 h;
(4) inoculating the bacterial liquid obtained from the seeding tank into a fermentation tank according to the inoculation amount of 5-10%, wherein the culture temperature is 25-28 ℃, the rotation speed of the fermentation tank is 80-120 r/min, and the ventilation ratio is 1: 0.6-1: 0.8, and culturing for 4-7 days;
wherein, the formula of the MEA solid culture medium is as follows: 12.75g/L of maltose, 2.75g/L of dextrin, 2.35g/L of glycerol, 0.78g/L of peptone and 15g/L of agar; the MEA liquid culture medium comprises: 12.75g/L of maltose, 2.75g/L of dextrin, 2.35g/L of glycerol and 0.78g/L of peptone; the culture medium formula of the seeding tank is as follows: 10g/L yeast extract, 20g/L peptone and 20g/L glucose; the formula of the fermentation tank culture medium is as follows: 15g/L of bran, 10g/L of straw powder, 10g/L of corn flour, 5g/L of ammonium sulfate, 0.3g/L of sodium chloride, 2g/L of dipotassium hydrogen phosphate and 0.25g/L of magnesium sulfate.
In any of the above schemes, preferably, the trichoderma longibrachiatum is purchased from China center for Industrial culture Collection of microorganisms, with the strain number CICC 40202.
The feed additive for preventing and treating cow mastitis provided by the invention has the following advantages and beneficial effects:
the fermented traditional Chinese medicine composition of the invention is composed of traditional Chinese medicines such as dandelion, honeysuckle, houttuynia cordata, Chinese violet, common andrographis herb, purslane, uniflower swisscentaury root, akebia stem, sweetgum fruit, cowherb seed, angelica, codonopsis pilosula, astragalus, scutellaria baicalensis, prepared rhizome of rehmannia, white paeony root, szechuan lovage rhizome, liquorice and the like,
the pharmacological actions of the traditional Chinese medicines are as follows:
dandelion: bitter and sweet in taste and cold in nature; it enters liver and stomach meridians. Clearing away heat and toxic materials, inducing diuresis to alleviate edema, and treating acute mastitis, lymphadenitis, scrofula, furunculosis, acute conjunctivitis, common cold with fever, acute tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis, and urinary tract infection.
Honeysuckle flower: sweet taste and cold nature; it enters heart and stomach meridians. Clearing heat and removing toxicity, and is mainly used for treating epidemic febrile disease, fever, heat-toxin and bloody dysentery, carbuncle, deep-rooted carbuncle, furunculosis and the like. Modern researches prove that the honeysuckle contains pharmacological active ingredients such as chlorogenic acid and luteolin glycoside, and has strong inhibitory effect on various pathogenic bacteria such as hemolytic streptococcus and staphylococcus aureus, and pathogenic viruses causing upper respiratory tract infection.
Houttuynia cordata: bitter taste and slightly cold nature; it enters lung, bladder and large intestine meridians. Clearing away heat and toxic materials, eliminating carbuncle, and expelling pus; it is indicated for heat stranguria, abscess, swelling and sore.
Herba Violae: bitter and pungent taste, cold in nature; it enters heart and liver meridians. Has effects of clearing away heat and toxic materials, cooling blood, and relieving swelling; it can be used for treating furuncle, superficial infection, mumps, scrofula, erysipelas, acute mastitis, intestinal abscess, damp-heat dysentery, jaundice, conjunctival congestion, swelling and pain, pharyngitis, and snake bite.
Andrographis paniculata: bitter taste and cold nature; it enters heart, lung, large intestine and bladder meridians. Has effects of clearing away heat and toxic materials, relieving inflammation, and relieving pain.
Purslane: sour in taste, cold in nature; it enters heart, liver, spleen and large intestine meridians. Has effects of clearing away heat and toxic materials, cooling blood, and stopping bleeding, and can be used for treating enteritis, acute arthritis, cystitis, urethritis, anal inflammation, hemorrhoid hemorrhage, etc.
And (4) uniflower swisscentaury root: bitter taste and cold nature; it enters stomach meridian. Has effects of clearing away heat and toxic materials, removing carbuncle, resolving hard mass, dredging channels, promoting lactation, relaxing muscles and tendons, and dredging collaterals.
B, wood through: bitter in taste and cool in nature, purging fire to promote diuresis, and promoting blood circulation, and can be used for treating dark urine, stranguria with turbid urine, edema, dysphoria with smothery sensation in chest, sore throat, pharyngalgia, muscular pain, amenorrhea, and galactostasis.
The road is through: pungent, bitter and neutral in flavor; it enters liver, stomach and bladder meridians. Has the main effects of dispelling wind, dredging collaterals and inducing diuresis for promoting lactation, and is mainly used for treating rheumatalgia, limb numbness, edema, dysuresia, galactostasis, breast distending pain and the like.
Seed of cowherb: bitter taste and mild nature; it enters liver and stomach meridians. The main effects are activating blood and stimulating the menstrual flow, promoting lactation and reducing swelling, inducing diuresis and treating stranguria, and the traditional Chinese medicine is used for amenorrhea, dysmenorrhea, galactorrhea, acute mastitis and gall, and stranguria with pain and astringency.
Chinese angelica: sweet taste and warm nature; it enters liver, heart and spleen meridians. Has effects in replenishing blood, promoting blood circulation, regulating menstruation, relieving pain, moistening dryness, smoothing intestine, resisting cancer, resisting aging, and enhancing immunity.
Codonopsis pilosula: sweet in taste and neutral in nature; it enters spleen and lung meridians. Has effects of invigorating spleen, replenishing qi, invigorating spleen, benefiting lung, regulating stomach function, promoting fluid production, eliminating phlegm, and relieving cough.
Astragalus root: sweet in flavor and slightly warm in nature; it enters lung, spleen, liver and kidney meridians. Has effects in invigorating qi, consolidating exterior, expelling toxin, expelling pus, promoting urination, and promoting granulation.
Scutellaria baicalensis: bitter taste and cold nature; it enters heart, lung, gallbladder and large intestine meridians. Has effects of clearing heat, eliminating dampness, clearing pathogenic fire, removing toxic substance, stopping bleeding, preventing miscarriage, and resisting various viruses, bacteria and fungi.
Prepared rhizome of rehmannia: sweet in flavor and slightly warm in nature; it enters liver and kidney meridians. Has effects of lowering blood pressure, inhibiting thrombosis, and replenishing blood.
White peony root: bitter and sour taste, slightly cold nature; it enters liver and spleen meridians. Has effects in nourishing blood, regulating menstruation, astringing yin, arresting sweating, softening liver, relieving pain, and suppressing liver yang.
Ligusticum wallichii: pungent taste and warm nature; it enters liver, gallbladder and pericardium meridians. Has effects of promoting blood circulation, activating qi-flowing, dispelling pathogenic wind and relieving pain.
Licorice root: sweet in taste and neutral in nature; they enter heart, lung, spleen and stomach meridians. Has effects of invigorating spleen and qi, clearing away heat and toxic materials, eliminating phlegm, relieving cough, and harmonizing the drugs.
In the traditional Chinese medicine composition, the three traditional Chinese medicines of the dandelion, the honeysuckle and the houttuynia cordata are monarch medicines, and the combination of the three medicines mainly aims at clearing heat and removing toxicity, relieving swelling and removing carbuncle, so that the medicine property is cold and cool, and the effect of clearing heat and removing toxicity is best; the Chinese violet, the purslane, the scutellaria baicalensis and the common andrographis herb assist three monarch drugs to strengthen the force of clearing heat and removing toxicity, the uniflower swisscentaury root, the akebiaquinata, the sweetgum fruit and the cowherb seed have the effects of dispelling wind and dredging collaterals, promoting blood circulation and treating dysmenorrhea, stimulating the menstrual flow and promoting lactation and the like, and the eight traditional Chinese medicines are ministerial medicines together, so that the effects of clearing heat and removing toxicity of the monarch drugs can be strengthened, and stimulating the menstrual flow and promoting lactation to dredge; the six traditional Chinese medicines are used as adjuvant medicines, have the effects of nourishing blood, activating blood, tonifying qi, promoting production of body fluid, strengthening exterior and tonifying qi, and can improve the immunity of the organism; the liquorice is used as a guiding drug, detoxifies and moderates the components of the traditional Chinese medicines, and regulates the efficacy of the medicines. Meanwhile, by utilizing the modern microbial fermentation technology, the trichoderma longibrachiatum which produces cellulase and hemicellulase at high yield is adopted to fully ferment the traditional Chinese medicine composition, so that the cell walls of the plant traditional Chinese medicines are degraded, the active ingredients of the traditional Chinese medicines are released, and the efficacy of the composition is improved.
The sodium humate is a natural high molecular compound, is black brown or grey black granules or powder, is formed by decomposing animal and plant residues, is widely present in peat, weathered coal and pond sludge, and has the main functions of enhancing the activity of various enzymes in animal bodies, promoting metabolism and improving immunity. The sodium humate is fed to milk cow in lactation period, and has effects of promoting increase of lymphocyte in blood, sterilizing, diminishing inflammation, reducing breast milk somatic cell, and preventing mastitis.
The cell wall of the saccharomyces cerevisiae is subjected to directional enzymolysis on saccharomyces cerevisiae cells to release substances with bacteriostatic activity, and the substances are extracted, wherein the main components of the substances are β -glucan and mannan oligosaccharide, so that the saccharomyces cerevisiae cell wall has the effects of promoting growth, improving immunity, adsorbing mycotoxin and the like, and can relieve the inhibition of the mycotoxin on the immune response reaction of dairy cows, thereby improving the resistance to pathogenic bacteria.
Zinc is a trace element necessary for dairy cows and is an important nutritional factor for protein keratinization and epithelial tissue hardening, keratin covers the surface of a milk duct of a dairy cow, the milk duct can secrete the keratin to seal the milk duct after each lactation, a large amount of keratin is lost in the processes of milk secretion and milking, and the keratin needs to be supplemented in time, otherwise, pathogenic bacteria are easily infected to cause somatic cell increase, so that the zinc supplement is very necessary, and as rumen microorganisms cannot utilize amino acid to chelate zinc, zinc glycinate can pass through rumen; in addition, zinc glycinate, copper and manganese glycinate induce liver to generate GSH-Px, so that free radicals are reduced, zinc and copper are active centers of Zn/Cu-SOD, and Mn is an active center of Mn-SOD, so that the oxidative stress can be reduced and the health level of the dairy cow can be improved by feeding organic trace elements of zinc, copper and manganese.
The natural resistance of the mammary gland to infection can be improved by adding vitamin E and selenium yeast into daily ration, the vitamin E can improve the humoral immunity and the cellular immunity of the dairy cow and can also enhance the killing capability of neutrophilic cells to staphylococcus aureus and escherichia coli, the selenium yeast and the vitamin E have a synergistic function, mastitis is reduced and the somatic cells of the milk are reduced along with the increase of the concentration of the selenium and the vitamin E in serum, the vitamin A is a nutrient substance necessary for maintaining the normal vision and the growth and the propagation of the dairy cow, the integrity of epithelial cell tissues can be maintained, an immunization line is formed to prevent the invasion of pathogenic bacteria, β -carotene can be converted into vitamin A in vivo to avoid excessive poisoning caused by the intake of the vitamin A, and the combination of the vitamin A and β -carotene can resist the mastitis pathogenic bacteria of staphylococcus aureus, escherichia coli and streptococcus uberis, the immunity is improved, and the incidence rate of the mastitis is reduced.
The fermented traditional Chinese medicine composition, the sodium humate, the cell wall of the saccharomyces cerevisiae, the compound organic trace elements, the compound vitamins and other components are combined, so that the cow mastitis is prevented and treated from different angles, and the immunity and the anti-infection capability of the cow organism are effectively improved.
Detailed Description
In order that the invention may be further understood, the invention will now be described in detail with reference to specific examples.
Example 1
The invention provides a feed additive for preventing and treating cow mastitis, which comprises the following components: 70 parts of fermented traditional Chinese medicine composition, 10 parts of sodium humate, 10 parts of saccharomyces cerevisiae cell wall, 5 parts of composite organic trace elements and 5 parts of composite vitamins.
The fermented traditional Chinese medicine composition is prepared from the following traditional Chinese medicines in parts by weight: 100-150 parts of dandelion, 100-150 parts of honeysuckle, 50-100 parts of houttuynia cordata, 30-80 parts of Chinese violet, 50-100 parts of common andrographis herb, 10-50 parts of purslane, 30-50 parts of uniflower swisscentaury root, 50-80 parts of akebia stem, 30-50 parts of beautiful sweetgum fruit, 30-50 parts of cowherb seed, 30-50 parts of angelica, 40-60 parts of codonopsis pilosula, 40-60 parts of astragalus membranaceus, 10-30 parts of scutellaria baicalensis, 30-60 parts of prepared rehmannia rhizome, 30-80 parts of white paeony root, 20-50 parts of ligusticum wallichii and 30-60 parts of liquorice.
Preferably, the fermented traditional Chinese medicine composition consists of the following traditional Chinese medicines in parts by weight: 130 parts of dandelion, 140 parts of honeysuckle, 80 parts of houttuynia cordata, 50 parts of Chinese violet, 60 parts of common andrographis herb, 30 parts of purslane, 50 parts of uniflower swisscentaury root, 50 parts of akebia stem, 30 parts of beautiful sweetgum fruit, 40 parts of cowherb seed, 50 parts of szechuan lovage rhizome and 60 parts of liquorice.
The composite organic trace element is prepared from the following raw materials in parts by weight: 20 parts of zinc glycinate, 10 parts of manganese glycinate, 20 parts of copper glycinate and 60 parts of yeast selenium.
The compound vitamin comprises the following raw materials, by weight, 50 parts of vitamin E, 20 parts of vitamin A and 30 parts of β -carotene.
The quality standard of the sodium humate is as follows: humic acid is more than or equal to 70 percent, and water is less than or equal to 10 percent;
the cell wall quality standard of the saccharomyces cerevisiae is that crude protein is less than or equal to 35 percent, mannan is more than or equal to 20 percent, β -glucan is more than or equal to 20 percent, and water is less than or equal to 8 percent;
the quality standard of the composite organic trace elements is as follows: zinc glycinate: glycine is more than or equal to 22 percent, Zn is more than or equal to 21 percent, and water content is less than or equal to 10 percent; manganese glycinate: mn is more than or equal to 10 percent, total glycine is more than or equal to 26 percent, and water content is less than or equal to 10 percent; copper glycine: cu is more than or equal to 22 percent, total glycine is more than or equal to 25 percent, and water content is less than or equal to 10 percent; selenium yeast: the organic selenium is more than or equal to 2000 mg/kg;
the quality standard of the compound vitamin is that the vitamin A acetate is more than or equal to 50 ten thousand IU/g, the water content is less than or equal to 5 percent, the vitamin E is more than or equal to 50 percent, the water content is less than or equal to 5 percent, β -carotene is more than or equal to 90 percent, and the water content is less than or equal to 5 percent.
The invention also provides a feed additive for preventing and treating cow mastitis, and the preparation method comprises the following steps:
(1) weighing the fermented traditional Chinese medicine composition, sodium humate, yeast cell walls and composite organic trace elements according to parts;
(2) the raw materials are added into a mixer for mixing for 5 minutes, wherein the coefficient of variation CV of the mixing uniformity is less than or equal to 5 percent.
Wherein, the preparation steps of the fermented traditional Chinese medicine composition are as follows:
(1) crushing the traditional Chinese medicines: pulverizing the above Chinese medicinal materials, and sieving with 60 mesh sieve;
(2) mixing: weighing the traditional Chinese medicine components according to the weight parts of the traditional Chinese medicines, premixing for 5 minutes by using a mixer, uniformly mixing the trichoderma longibrachiatum liquid with water, and pumping the mixture into the mixer by using a water pump to mix with the traditional Chinese medicines;
(3) solid fermentation: feeding the mixed materials into a fermentation chamber, controlling the temperature of the fermentation chamber to be 25 ℃ and the humidity to be 40%, turning over the materials once every 6 hours, and fermenting for 5 days;
(4) airflow drying: adopting boiling drying, wherein the drying temperature is controlled at 65 ℃;
the preparation method of the trichoderma longibrachiatum liquid used in the fermented traditional Chinese medicine composition comprises the following steps:
(1) strain activation
Picking a ring of trichoderma longibrachiatum inclined plane seeds, inoculating the seeds into an MEA liquid culture medium, and carrying out shake culture at 25 ℃ and 200r/min for 3 d; then inoculating the bacterial liquid into an MEA solid slant culture medium, standing and culturing for 5d, and growing light green spores on the slant;
(2) preparation of spore suspension
Washing the inclined plane of the trichoderma longibrachiatum by using 2% of tween 80 to obtain a suspension, putting the suspension into a sterile triangular flask with glass beads, putting the triangular flask into a 200r/min shaking table for oscillation, and scattering spores to obtain a spore suspension;
(1)3 seed liquid preparation
Inoculating the spore suspension obtained in the step (2) into a seeding tank according to the inoculation amount of 5%, wherein the culture temperature is 25 ℃, the rotation speed of the seeding tank is 150-200 r/min, the ventilation ratio is 1: 0.7-1: 1, and the culture time is 48 h;
(4) cultivation in fermenter
Inoculating the bacterial liquid obtained from the seeding tank into a fermentation tank according to the inoculation amount of 5%, wherein the culture temperature is 25 ℃, the rotation speed of the fermentation tank is 80-120 r/min, and the ventilation ratio is 1: 0.6-1: 0.8, and the culture time is 4 days.
The formula of the MEA solid culture medium is as follows: 12.75g/L of maltose, 2.75g/L of dextrin, 2.35g/L of glycerol, 0.78g/L of peptone and 15g/L of agar; the MEA liquid culture medium comprises: 12.75g/L of maltose, 2.75g/L of dextrin, 2.35g/L of glycerol and 0.78g/L of peptone; the culture medium formula of the seeding tank is as follows: 10g/L yeast extract, 20g/L peptone and 20g/L glucose; the formula of the fermentation tank culture medium is as follows: 15g/L of bran, 10g/L of straw powder, 10g/L of corn flour, 5g/L of ammonium sulfate, 0.3g/L of sodium chloride, 2g/L of dipotassium hydrogen phosphate and 0.25g/L of magnesium sulfate. The trichoderma longibrachiatum is purchased from China center for industrial microorganism culture collection with the strain number CICC 40202.
Example two
The invention provides a feed additive for preventing and treating cow mastitis, which comprises the following components: 82 parts of fermented traditional Chinese medicine composition, 5 parts of sodium humate, 5 parts of saccharomyces cerevisiae cell wall, 4 parts of composite organic trace elements and 4 parts of composite vitamins.
The fermented traditional Chinese medicine composition is prepared from the following traditional Chinese medicines in parts by weight: 100-150 parts of dandelion, 100-150 parts of honeysuckle, 50-100 parts of houttuynia cordata, 30-80 parts of Chinese violet, 50-100 parts of common andrographis herb, 10-50 parts of purslane, 30-50 parts of uniflower swisscentaury root, 50-80 parts of akebia stem, 30-50 parts of beautiful sweetgum fruit, 30-50 parts of cowherb seed, 30-50 parts of angelica, 40-60 parts of codonopsis pilosula, 40-60 parts of astragalus membranaceus, 10-30 parts of scutellaria baicalensis, 30-60 parts of prepared rehmannia rhizome, 30-80 parts of white paeony root, 20-50 parts of ligusticum wallichii and 30-60 parts of liquorice.
Preferably, the fermented traditional Chinese medicine composition consists of the following traditional Chinese medicines in parts by weight: 130 parts of dandelion, 140 parts of honeysuckle, 80 parts of houttuynia cordata, 50 parts of Chinese violet, 60 parts of common andrographis herb, 30 parts of purslane, 50 parts of uniflower swisscentaury root, 50 parts of akebia stem, 30 parts of beautiful sweetgum fruit, 40 parts of cowherb seed, 50 parts of szechuan lovage rhizome and 60 parts of liquorice.
The composite organic trace element is prepared from the following raw materials in parts by weight: 25 parts of zinc glycinate, 15 parts of manganese glycinate, 18 parts of copper glycinate and 42 parts of yeast selenium.
The compound vitamin comprises, by weight, 55 parts of vitamin E, 25 parts of vitamin A and 20 parts of β -carotene.
The quality standard of the sodium humate is as follows: humic acid is more than or equal to 70 percent, and water is less than or equal to 10 percent;
the cell wall quality standard of the saccharomyces cerevisiae is that crude protein is less than or equal to 35 percent, mannan is more than or equal to 20 percent, β -glucan is more than or equal to 20 percent, and water is less than or equal to 8 percent;
the quality standard of the composite organic trace elements is as follows: zinc glycinate: glycine is more than or equal to 22 percent, Zn is more than or equal to 21 percent, and water content is less than or equal to 10 percent; manganese glycinate: mn is more than or equal to 10 percent, total glycine is more than or equal to 26 percent, and water content is less than or equal to 10 percent; copper glycine: cu is more than or equal to 22 percent, total glycine is more than or equal to 25 percent, and water content is less than or equal to 10 percent; selenium yeast: the organic selenium is more than or equal to 2000 mg/kg;
the quality standard of the compound vitamin is that the vitamin A acetate is more than or equal to 50 ten thousand IU/g, the water content is less than or equal to 5 percent, the vitamin E is more than or equal to 50 percent, the water content is less than or equal to 5 percent, β -carotene is more than or equal to 90 percent, and the water content is less than or equal to 5 percent.
The invention also provides the feed additive for preventing and treating the cow mastitis, and the preparation method comprises the following steps:
(1) weighing the fermented traditional Chinese medicine composition, sodium humate, yeast cell walls and composite organic trace elements according to the weight parts;
(2) mixing the raw materials in a mixer for 5 minutes, wherein the coefficient of variation CV of the mixing uniformity is less than or equal to 5 percent.
Wherein, the preparation steps of the fermented traditional Chinese medicine composition are as follows:
(1) crushing the traditional Chinese medicines: pulverizing the above Chinese medicinal materials, and sieving with 60 mesh sieve;
(2) mixing: weighing the traditional Chinese medicine components according to the weight parts of the traditional Chinese medicines, premixing for 5 minutes by using a mixer, uniformly mixing the trichoderma longibrachiatum liquid with water, and pumping the mixture into the mixer by using a water pump to mix with the traditional Chinese medicines.
(3) Solid fermentation: and (3) feeding the mixed materials into a fermentation chamber, controlling the temperature of the fermentation chamber to be 27 ℃ and the humidity to be 50%, turning the piles once every 6 hours, and fermenting for 5 days.
(4) Airflow drying: boiling drying is adopted, and the drying temperature is controlled at 70 ℃.
The preparation method of the trichoderma longibrachiatum liquid used in the fermented traditional Chinese medicine composition comprises the following steps:
(1) strain activation
Picking a ring of Trichoderma longibrachiatum (Trichoderma longibrachiatum) inclined plane seeds, inoculating the seeds into an MEA liquid culture medium, and carrying out shake culture at 25 ℃ and 200r/min for 3 d; then inoculating the bacterial liquid into an MEA solid slant culture medium, standing and culturing for 6d, and growing light green spores on the slant;
(2) preparation of spore suspension
Washing the inclined plane of the trichoderma longibrachiatum by using 2% of tween 80 to obtain a suspension, putting the suspension into a sterile triangular flask with glass beads, putting the triangular flask into a 200r/min shaking table for oscillation, and scattering spores to obtain a spore suspension;
(3) seed liquid preparation
Inoculating the spore suspension obtained in the step (2) into a seeding tank according to the inoculation amount of 5%, wherein the culture temperature is 26 ℃, the rotation speed of the seeding tank is 150-200 r/min, the ventilation ratio is 1: 0.7-1: 1, and the culture time is 72 h;
(4) cultivation in fermenter
Inoculating the bacterial liquid obtained from the seeding tank into a fermentation tank according to the inoculation amount of 5-10%, wherein the culture temperature is 25-28 ℃, the rotation speed of the fermentation tank is 80-120 r/min, and the ventilation ratio is 1: 0.6-1: 0.8, and the culture time is 7 days.
The formula of the MEA solid culture medium is as follows: 12.75g/L of maltose, 2.75g/L of dextrin, 2.35g/L of glycerol, 0.78g/L of peptone and 15g/L of agar; the MEA liquid culture medium comprises: 12.75g/L of maltose, 2.75g/L of dextrin, 2.35g/L of glycerol and 0.78g/L of peptone; the culture medium formula of the seeding tank is as follows: 10g/L yeast extract, 20g/L peptone and 20g/L glucose; the formula of the fermentation tank culture medium is as follows: 15g/L of bran, 10g/L of straw powder, 10g/L of corn flour, 5g/L of ammonium sulfate, 0.3g/L of sodium chloride, 2g/L of dipotassium hydrogen phosphate and 0.25g/L of magnesium sulfate. The trichoderma longibrachiatum is purchased from China center for industrial microorganism culture collection with the strain number CICC 40202.
EXAMPLE III
The invention provides a feed additive for preventing and treating cow mastitis, which comprises the following components: 90 parts of fermented traditional Chinese medicine composition, 5 parts of sodium humate, 5 parts of saccharomyces cerevisiae cell wall, 3 parts of composite organic trace elements and 3 parts of composite vitamins.
The fermented traditional Chinese medicine composition is prepared from the following traditional Chinese medicines in parts by weight: 100-150 parts of dandelion, 100-150 parts of honeysuckle, 50-100 parts of houttuynia cordata, 30-80 parts of Chinese violet, 50-100 parts of common andrographis herb, 10-50 parts of purslane, 30-50 parts of uniflower swisscentaury root, 50-80 parts of akebia stem, 30-50 parts of beautiful sweetgum fruit, 30-50 parts of cowherb seed, 30-50 parts of angelica, 40-60 parts of codonopsis pilosula, 40-60 parts of astragalus membranaceus, 10-30 parts of scutellaria baicalensis, 30-60 parts of prepared rehmannia rhizome, 30-80 parts of white paeony root, 20-50 parts of ligusticum wallichii and 30-60 parts of liquorice.
Preferably, the fermented traditional Chinese medicine composition consists of the following traditional Chinese medicines in parts by weight: 130 parts of dandelion, 140 parts of honeysuckle, 80 parts of houttuynia cordata, 50 parts of Chinese violet, 60 parts of common andrographis herb, 30 parts of purslane, 50 parts of uniflower swisscentaury root, 50 parts of akebia stem, 30 parts of beautiful sweetgum fruit, 40 parts of cowherb seed, 50 parts of szechuan lovage rhizome and 60 parts of liquorice.
The composite organic trace element is prepared from the following raw materials in parts by weight: 25 parts of zinc glycinate, 15 parts of manganese glycinate, 18 parts of copper glycinate and 42 parts of yeast selenium.
The compound vitamin comprises, by weight, 55 parts of vitamin E, 25 parts of vitamin A and 20 parts of β -carotene.
The quality standard of the sodium humate is as follows: humic acid is more than or equal to 70 percent, and water is less than or equal to 10 percent;
the cell wall quality standard of the saccharomyces cerevisiae is that crude protein is less than or equal to 35 percent, mannan is more than or equal to 20 percent, β -glucan is more than or equal to 20 percent, and water is less than or equal to 8 percent;
the quality standard of the composite organic trace elements is as follows: zinc glycinate: glycine is more than or equal to 22 percent, Zn is more than or equal to 21 percent, and water content is less than or equal to 10 percent; manganese glycinate: mn is more than or equal to 10 percent, total glycine is more than or equal to 26 percent, and water content is less than or equal to 10 percent; copper glycine: cu is more than or equal to 22 percent, total glycine is more than or equal to 25 percent, and water content is less than or equal to 10 percent; selenium yeast: the organic selenium is more than or equal to 2000 mg/kg;
the quality standard of the compound vitamin is that the vitamin A acetate is more than or equal to 50 ten thousand IU/g, the water content is less than or equal to 5 percent, the vitamin E is more than or equal to 50 percent, the water content is less than or equal to 5 percent, β -carotene is more than or equal to 90 percent, and the water content is less than or equal to 5 percent.
The invention also provides the feed additive for preventing and treating the cow mastitis, and the preparation method comprises the following steps:
(1) weighing the fermented traditional Chinese medicine composition, sodium humate, yeast cell walls and composite organic trace elements according to the weight parts;
(2) mixing the raw materials in a mixer for 5 minutes, wherein the coefficient of variation CV of the mixing uniformity is less than or equal to 5 percent.
Wherein, the preparation steps of the fermented traditional Chinese medicine composition are as follows:
(1) crushing the traditional Chinese medicines: pulverizing the above Chinese medicinal materials, and sieving with 60 mesh sieve;
(2) mixing: weighing the traditional Chinese medicine components according to the weight parts of the traditional Chinese medicines, premixing for 5 minutes by using a mixer, uniformly mixing the trichoderma longibrachiatum liquid with water, and pumping the mixture into the mixer by using a water pump to mix with the traditional Chinese medicines.
(3) Solid fermentation: and (3) feeding the mixed materials into a fermentation chamber, controlling the temperature of the fermentation chamber to be 30 ℃ and the humidity to be 60%, turning over the materials once every 6 hours, and fermenting for 5 days.
(4) Airflow drying: adopting boiling drying, and controlling the drying temperature at 80 ℃.
The preparation method of the trichoderma longibrachiatum liquid used in the fermented traditional Chinese medicine composition comprises the following steps:
(1) strain activation
Picking a ring of Trichoderma longibrachiatum (Trichoderma longibrachiatum) inclined plane seeds, inoculating the seeds into an MEA liquid culture medium, and carrying out shake culture at 25 ℃ and 200r/min for 3 d; then, inoculating the bacterial liquid into an MEA (membrane electrode assembly) solid slant culture medium, standing and culturing for 5-7 d, and growing light green spores on the slant;
(2) preparation of spore suspension
Washing the inclined plane of the trichoderma longibrachiatum by using 2% of tween 80 to obtain a suspension, putting the suspension into a sterile triangular flask with glass beads, putting the triangular flask into a 200r/min shaking table for oscillation, and scattering spores to obtain a spore suspension;
(3) seed liquid preparation
Inoculating the spore suspension obtained in the step (2) into a seeding tank according to the inoculation amount of 5%, wherein the culture temperature is 28 ℃, the rotation speed of the seeding tank is 150-200 r/min, the ventilation ratio is 1: 0.7-1: 1, and the culture time is 60 h;
(4) cultivation in fermenter
Inoculating the bacterial liquid obtained from the seeding tank into a fermentation tank according to the inoculation amount of 5-10%, wherein the culture temperature is 25-28 ℃, the rotation speed of the fermentation tank is 80-120 r/min, and the ventilation ratio is 1: 0.6-1: 0.8, and the culture time is 5 days.
The formula of the MEA solid culture medium is as follows: 12.75g/L of maltose, 2.75g/L of dextrin, 2.35g/L of glycerol, 0.78g/L of peptone and 15g/L of agar; the MEA liquid culture medium comprises: 12.75g/L of maltose, 2.75g/L of dextrin, 2.35g/L of glycerol and 0.78g/L of peptone; the culture medium formula of the seeding tank is as follows: 10g/L yeast extract, 20g/L peptone and 20g/L glucose; the formula of the fermentation tank culture medium is as follows: 15g/L of bran, 10g/L of straw powder, 10g/L of corn flour, 5g/L of ammonium sulfate, 0.3g/L of sodium chloride, 2g/L of dipotassium hydrogen phosphate and 0.25g/L of magnesium sulfate. The trichoderma longibrachiatum is purchased from China center for industrial microorganism culture collection with the strain number CICC 40202.
Performance testing
1 protocol
Selecting 40 Holstein cows in mid-lactation period, dividing the experiment into 2 groups, wherein 20 healthy cows, 20 stealth mastitis cows (the number of test body cells is more than 30 ten thousand/ml) in a DHI method, 10 days in a pre-feeding period, 30 days in an experimental period, and 0.5 percent of additive addition amount, collecting milk samples at 8:00, 14:00 and 18:00 before and after the experiment, preserving the milk samples in a 4-degree refrigerator, measuring the body cells, the milk fat rate, the protein rate, the lipoprotein ratio and the urea nitrogen, and counting the average milk yield of each cow.
2 results
2.1 Effect of additives on the relevant indices of healthy cows
As can be seen from Table 1, the difference between the change of somatic cells and the change of milk yield of healthy cows is not significant (P >0.05) before and after the additive is added, and the effect of the additive on the increase of the milk yield is proved to be insignificant; the milk fat rate, the protein rate, the lipoprotein ratio and the urea nitrogen are remarkably increased (P is less than 0.01).
TABLE 1 Effect of additives on relevant indices of healthy cows
Index (I) 0d 30d
Somatic cell/(10)4·ml-1) 10.29±7.32 10.05±7.69
Milk fat percentage/%) 3.97±0.66 4.78±0.62**
Protein rate/% 3.48±0.41 3.66±0.34**
Ratio of fat to egg 1.14±0.27 1.29±0.31**
Urea nitrogen/(mg. dL) 11.67±2.67 13.65±2.23**
Milk yield/kg 29.81±7.01 29.67±6.99
2.2 Effect of the additive on recessive mastitis group cows
As can be seen from Table 2, the difference of milk yield before and after the additive is used is not significant (P >0.05), the ratio of protein rate to lipoprotein is remarkably increased (P <0.05), the milk fat rate is remarkably increased (P <0.01), the somatic cells are remarkably reduced (P <0.01), and the somatic cells reach the standard of healthy cows.
TABLE 2 Effect of additives on cow-related indices in the group of recessive mastitis
Index (I) 0d 30d
Somatic cell/(10)4·ml-1) 62.5±10.21 14.00±4.20**
Milk fat percentage/%) 4.01±0.78 4.65±0.54**
Protein rate/% 3.35±0.36 3.50±0.29*
Ratio of fat to egg 1.20±0.21 1.3±0.28*
Urea nitrogen/(mg. dL) 11.11±2.52 13.46±2.35**
Milk yield/kg 29.14±7.82 28.26±7.55
3 conclusion
The test results show that after the additive is added, the additive has no adverse effect on normal cows, and can improve the milk quality indexes of the normal cows, such as the milk fat percentage, the milk protein and the like; after the additive is added into the dairy cows with recessive mastitis, the milk quality is improved, and the somatic cells are remarkably reduced to a normal level, which shows that the additive has remarkable effect of reducing the somatic cells of the dairy cows with mastitis, and can also improve the milk quality.
It will be understood by those skilled in the art that the feed additive for preventing and treating bovine mastitis and the preparation method thereof according to the present invention include the summary and the detailed description of the present invention, which are described above, are limited to space and are not described in the respective schemes of these combinations for the sake of brevity. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (10)

1. The feed additive for preventing and treating cow mastitis is characterized by comprising the following raw materials in parts by weight: 70-90 parts of fermented traditional Chinese medicine composition, 5-10 parts of sodium humate, 5-10 parts of saccharomyces cerevisiae cell walls, 3-5 parts of composite organic trace elements and 3-5 parts of composite vitamins.
2. The feed additive for preventing and treating cow mastitis according to claim 1, wherein the fermented Chinese herbal medicine composition is prepared from the following Chinese herbal medicines in parts by weight: 100-150 parts of dandelion, 100-150 parts of honeysuckle, 50-100 parts of houttuynia cordata, 30-80 parts of Chinese violet, 50-100 parts of common andrographis herb, 10-50 parts of purslane, 30-50 parts of uniflower swisscentaury root, 50-80 parts of akebia stem, 30-50 parts of beautiful sweetgum fruit, 30-50 parts of cowherb seed, 30-50 parts of angelica, 40-60 parts of codonopsis pilosula, 40-60 parts of astragalus membranaceus, 10-30 parts of scutellaria baicalensis, 30-60 parts of prepared rehmannia rhizome, 30-80 parts of white paeony root, 20-50 parts of ligusticum wallichii and 30-60 parts of liquorice.
3. The feed additive for preventing and treating cow mastitis according to claim 2, wherein the fermented Chinese herbal medicine composition is prepared from the following Chinese herbal medicines in parts by weight: the fermented traditional Chinese medicine composition is prepared from the following traditional Chinese medicines in parts by weight: 130 parts of dandelion, 140 parts of honeysuckle, 80 parts of houttuynia cordata, 50 parts of Chinese violet, 60 parts of common andrographis herb, 30 parts of purslane, 50 parts of uniflower swisscentaury root, 50 parts of akebia stem, 30 parts of beautiful sweetgum fruit, 40 parts of cowherb seed, 50 parts of szechuan lovage rhizome and 60 parts of liquorice.
4. The feed additive for preventing and treating cow mastitis according to claim 1, wherein the compound organic trace element is prepared from the following raw materials in parts by weight: 20-30 parts of zinc glycinate, 10-20 parts of manganese glycinate, 10-20 parts of copper glycinate and 40-60 parts of yeast selenium.
5. The feed additive for preventing and treating cow mastitis according to claim 1, wherein the compound vitamin comprises, by weight, 50-60 parts of vitamin E, 20-30 parts of vitamin A, and 20-30 parts of β -carotene.
6. The feed additive for preventing and treating cow mastitis according to claim 1, wherein the quality standards of sodium humate are that humic acid is more than or equal to 70% and water is less than or equal to 10%, the quality standards of cell walls of saccharomyces cerevisiae are that crude protein is less than or equal to 35%, mannan is more than or equal to 20%, β -glucan is more than or equal to 20% and water is less than or equal to 8%, the quality standards of composite organic trace elements are that zinc glycinate is more than or equal to 22% glycine, Zn is more than or equal to 21% and water is less than or equal to 10%, manganese glycinate is more than or equal to 10%, total glycine is more than or equal to 26% and water is less than or equal to 10%, copper glycinate is more than or equal to 22%, total glycine is more than or equal to 25% and water is less than or equal to 10%, yeast selenium is more than or equal to 2000mg/kg, and the quality standards of composite vitamins are that vitamin A acetate is more than or equal to.
7. A preparation method of a feed additive for preventing and treating cow mastitis comprises the following steps in sequence:
(1) weighing fermented traditional Chinese medicine composition, sodium humate, cell walls of saccharomyces cerevisiae, compound organic trace elements and compound vitamins according to parts;
(2) adding the raw materials into a mixer, starting the mixer to mix for 5 minutes to obtain the material, wherein the coefficient of variation CV of the mixing uniformity is less than or equal to 5 percent.
8. The preparation method of the feed additive for preventing and treating cow mastitis according to claim 7, wherein the preparation steps of the fermented traditional Chinese medicine composition are as follows:
(1) pulverizing the above Chinese medicinal materials, and sieving with 60 mesh sieve;
(2) weighing the traditional Chinese medicine components according to the weight parts of the traditional Chinese medicines, adding the traditional Chinese medicine components into a mixer, premixing for 5 minutes, uniformly mixing trichoderma longibrachiatum liquid with water, adding the mixture into the mixer, and mixing with the traditional Chinese medicines;
(3) feeding the mixed materials into a fermentation chamber, controlling the temperature of the fermentation chamber to be 25-30 ℃ and the humidity to be 40-60%, turning over every 6 hours, and fermenting for 5 d;
(4) and drying the fermented material by adopting a boiling drying method, controlling the drying temperature to be 65-80 ℃, and drying to obtain the fermented traditional Chinese medicine composition.
9. The preparation method of the feed additive for preventing and treating cow mastitis according to claim 8, wherein the preparation of trichoderma longibrachiatum liquid comprises the following steps:
(1) picking a ring of trichoderma longibrachiatum inclined plane seeds, inoculating the seeds into an MEA liquid culture medium, performing shake culture at 25 ℃ at 200r/min for 3d, then inoculating a bacterial liquid into an MEA solid inclined plane culture medium, and performing standing culture for 5-7 d to grow light green spores on the inclined plane;
(2) washing the inclined plane of the trichoderma longibrachiatum by using 2% of tween 80 to obtain a suspension, putting the suspension into a sterile triangular flask with glass beads, putting the triangular flask into a 200r/min shaking table for oscillation, and scattering spores to obtain a spore suspension;
(3) inoculating the spore suspension obtained in the step (2) into a seeding tank according to the inoculation amount of 5%, wherein the culture temperature is 25-28 ℃, the rotation speed of the seeding tank is 150-200 r/min, the ventilation ratio is 1: 0.7-1: 1, and the culture time is 48-72 h;
(4) inoculating the bacterial liquid obtained from the seeding tank into a fermentation tank according to the inoculation amount of 5-10%, wherein the culture temperature is 25-28 ℃, the rotation speed of the fermentation tank is 80-120 r/min, and the ventilation ratio is 1: 0.6-1: 0.8, and culturing for 4-7 days;
wherein, the formula of the MEA solid culture medium is as follows: 12.75g/L of maltose, 2.75g/L of dextrin, 2.35g/L of glycerol, 0.78g/L of peptone and 15g/L of agar; the MEA liquid culture medium comprises: 12.75g/L of maltose, 2.75g/L of dextrin, 2.35g/L of glycerol and 0.78g/L of peptone; the culture medium formula of the seeding tank is as follows: 10g/L yeast extract, 20g/L peptone and 20g/L glucose; the formula of the fermentation tank culture medium is as follows: 15g/L of bran, 10g/L of straw powder, 10g/L of corn flour, 5g/L of ammonium sulfate, 0.3g/L of sodium chloride, 2g/L of dipotassium hydrogen phosphate and 0.25g/L of magnesium sulfate.
10. The method for preparing a feed additive for preventing and treating bovine mastitis according to claim 9, wherein the trichoderma longibrachiatum is purchased from China center for Industrial culture Collection of microorganisms, and has the strain number CICC 40202.
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CN114128799A (en) * 2021-12-16 2022-03-04 唐山拓普生物科技有限公司 Feed additive for improving immunity of lactating cows and preparation method thereof

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Application publication date: 20200417