CN105831394A - Preparation method of microbial fermentation feed material - Google Patents
Preparation method of microbial fermentation feed material Download PDFInfo
- Publication number
- CN105831394A CN105831394A CN201610183590.5A CN201610183590A CN105831394A CN 105831394 A CN105831394 A CN 105831394A CN 201610183590 A CN201610183590 A CN 201610183590A CN 105831394 A CN105831394 A CN 105831394A
- Authority
- CN
- China
- Prior art keywords
- weight
- cfu
- raw material
- fermentation
- lactobacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 79
- 230000004151 fermentation Effects 0.000 title claims abstract description 78
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 239000000463 material Substances 0.000 title abstract description 13
- 230000000813 microbial effect Effects 0.000 title abstract 2
- 239000002994 raw material Substances 0.000 claims abstract description 81
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 69
- 241000186660 Lactobacillus Species 0.000 claims abstract description 41
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 41
- 238000000034 method Methods 0.000 claims abstract description 29
- 235000019750 Crude protein Nutrition 0.000 claims abstract description 24
- 240000008042 Zea mays Species 0.000 claims abstract description 23
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 23
- 229920002261 Corn starch Polymers 0.000 claims abstract description 19
- 239000008120 corn starch Substances 0.000 claims abstract description 19
- 235000016068 Berberis vulgaris Nutrition 0.000 claims abstract description 16
- 241000335053 Beta vulgaris Species 0.000 claims abstract description 16
- 235000013379 molasses Nutrition 0.000 claims abstract description 16
- 239000002131 composite material Substances 0.000 claims abstract description 15
- 210000000582 semen Anatomy 0.000 claims description 45
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 43
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 42
- 241000193755 Bacillus cereus Species 0.000 claims description 37
- 235000013339 cereals Nutrition 0.000 claims description 27
- 239000002054 inoculum Substances 0.000 claims description 24
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 22
- 239000000126 substance Substances 0.000 claims description 22
- 241000894006 Bacteria Species 0.000 claims description 20
- 239000000843 powder Substances 0.000 claims description 19
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 18
- 235000019784 crude fat Nutrition 0.000 claims description 17
- 239000000835 fiber Substances 0.000 claims description 17
- 239000002253 acid Substances 0.000 claims description 13
- 239000003818 cinder Substances 0.000 claims description 13
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 12
- 235000009973 maize Nutrition 0.000 claims description 12
- 244000063299 Bacillus subtilis Species 0.000 claims description 9
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 9
- 241000235646 Cyberlindnera jadinii Species 0.000 claims description 9
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 9
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 9
- 239000004310 lactic acid Substances 0.000 claims description 9
- 235000014655 lactic acid Nutrition 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 8
- 238000004806 packaging method and process Methods 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- 241000193749 Bacillus coagulans Species 0.000 claims description 6
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 6
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 6
- 229940054340 bacillus coagulans Drugs 0.000 claims description 6
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 6
- 241000194108 Bacillus licheniformis Species 0.000 claims description 5
- 244000005700 microbiome Species 0.000 claims description 5
- 241000186840 Lactobacillus fermentum Species 0.000 claims description 4
- 101710089042 Demethyl-4-deoxygadusol synthase Proteins 0.000 abstract description 15
- 239000000047 product Substances 0.000 abstract description 13
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract description 11
- 235000005822 corn Nutrition 0.000 abstract description 11
- 239000006227 byproduct Substances 0.000 abstract description 8
- 230000008901 benefit Effects 0.000 abstract description 7
- 241000193830 Bacillus <bacterium> Species 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 235000016709 nutrition Nutrition 0.000 abstract description 5
- 239000002699 waste material Substances 0.000 abstract description 5
- 230000001580 bacterial effect Effects 0.000 abstract description 3
- 108090000623 proteins and genes Proteins 0.000 abstract description 3
- 102000004169 proteins and genes Human genes 0.000 abstract description 3
- 235000013312 flour Nutrition 0.000 abstract description 2
- 241000235342 Saccharomycetes Species 0.000 abstract 1
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 239000011159 matrix material Substances 0.000 abstract 1
- 230000035764 nutrition Effects 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 239000002609 medium Substances 0.000 description 8
- 239000001963 growth medium Substances 0.000 description 7
- 238000007747 plating Methods 0.000 description 7
- 238000011534 incubation Methods 0.000 description 6
- 239000010977 jade Substances 0.000 description 6
- 230000033228 biological regulation Effects 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 240000007594 Oryza sativa Species 0.000 description 4
- 235000007164 Oryza sativa Nutrition 0.000 description 4
- 239000001888 Peptone Substances 0.000 description 4
- 108010080698 Peptones Proteins 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000001035 drying Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 235000019319 peptone Nutrition 0.000 description 4
- 235000009566 rice Nutrition 0.000 description 4
- 239000011343 solid material Substances 0.000 description 4
- 238000012549 training Methods 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000002372 labelling Methods 0.000 description 3
- 150000007524 organic acids Chemical class 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 210000004243 sweat Anatomy 0.000 description 3
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000000740 bleeding effect Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 235000021050 feed intake Nutrition 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 239000002932 luster Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 230000035790 physiological processes and functions Effects 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 235000019991 rice wine Nutrition 0.000 description 2
- 238000011218 seed culture Methods 0.000 description 2
- 238000012807 shake-flask culturing Methods 0.000 description 2
- 239000002893 slag Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- 206010007134 Candida infections Diseases 0.000 description 1
- 244000086443 Craterellus fallax Species 0.000 description 1
- 235000007926 Craterellus fallax Nutrition 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- 240000003183 Manihot esculenta Species 0.000 description 1
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 1
- 241000736262 Microbiota Species 0.000 description 1
- 102000013275 Somatomedins Human genes 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 description 1
- FUFJGUQYACFECW-UHFFFAOYSA-L calcium hydrogenphosphate Chemical compound [Ca+2].OP([O-])([O-])=O FUFJGUQYACFECW-UHFFFAOYSA-L 0.000 description 1
- 239000000920 calcium hydroxide Substances 0.000 description 1
- 229910001861 calcium hydroxide Inorganic materials 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 201000003984 candidiasis Diseases 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000019700 dicalcium phosphate Nutrition 0.000 description 1
- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 235000015141 kefir Nutrition 0.000 description 1
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 1
- 229940012969 lactobacillus fermentum Drugs 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 235000003715 nutritional status Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 125000001477 organic nitrogen group Chemical group 0.000 description 1
- 235000020265 peanut milk Nutrition 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 230000025366 tissue development Effects 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 235000014101 wine Nutrition 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/143—Fermentum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a preparation method of a microbial fermentation feed material. The method includes: inoculating a composite bacterial strain to fermentation raw materials for fermentation, wherein the fermentation raw materials contain one or more of corn starch residue, beet molasses, corn distillers solubles, citric acid distillers, corn flour, bad DDGS and shortcreting corn skin; and the fermentation raw materials on the basis of dry matrix contains 20 wt% of crude protein, and the composite strain contains lactobacillus, bacillus and saccharomycetes. The method uses industrial by-products as fermentation raw materials for the production of high energy feed or high protein feed material, organically integrates by-products, changes wastes into treasures, and makes the economic benefits of the product to the maximum; the composite bacterial strain for fermentation improves the nutrition of feed and absorption rate, and can greatly improve the nutritional value of the product. In addition, the preparation method has the advantages of simple production process, low cost and short production cycle, and is easy to mass production.
Description
Technical field
The present invention relates to the preparation method of feedstuff, in particular it relates to a kind of fermentative feedstuff of microbe is former
The preparation method of material.
Background technology
In feed industry, Applied Biotechnology is increasingly paid attention to, and reason is that it can be turned waste into wealth,
Sufficiently utilize trade waste, the most both provided abundant feed resource, and alleviated again environmental pollution.
Biological feedstuff is by its high-quality, the albumen of high-load and the advantage of abundant bioactive substance, to alleviation
The situation that feedstuff is nervous, promotes the development of feed industry and animal husbandry will play huge promotion and makees
With.Yeast cells contains rich in protein, aminoacid and multivitamin, especially vitamin B group
And the various digestive enzymes of content more horn of plenty.The inorganic nitrogen-sourced organic nitrogen that is converted into can be improved feedstuff
Protein content.Bacillus cereus can in animal intestinal growth and breeding, can produce multiple nutrients material as dimension
Raw element, aminoacid, organic acid, somatomedin etc., participate in the metabolism of animal body, for animal
Body provides nutrient substance.Lactobacillus ferment produces the materials such as organic acid, specific enzymes system, acid rhzomorph to be had
Special physiological function, can stimulate tissue development, to the nutritional status of body, physiological function, immunoreation
With generation effects such as stress.
With Semen Maydis, manioc waste as raw material, during producing the products such as citric acid, lysine, ethanol,
A lot of class side-product can be produced, such as citric acid grain, corn starch residue, Semen Maydis pulp, skin of Semen Maydis etc..Secondary
When product directly processes or sells, its market value does not obtain best embodiment.
Summary of the invention
It is an object of the invention to provide the preparation method of a kind of fermentative feedstuff of microbe raw material, by this preparation side
The fermentative feedstuff of microbe raw material that method prepares, the method for the present invention can utilize industrial by-products as fermentation
Raw material, makes high-energy feed or high-protein feed raw material, makes product economy maximizing the benefits.
According to the first aspect of the invention, the invention provides the preparation of a kind of fermentative feedstuff of microbe raw material
Method, the method includes being inoculated in fermentation raw material composite bacteria fermenting, it is characterised in that institute
Stating fermentation raw material, to contain corn starch residue, beet molasses, maize alcohol lees soluble substance, citric acid poor, beautiful
Rice flour, distiller's dried grain and soluble substance (hereinafter also referred to DDGS) thereof and the one or many whitewashed in skin of Semen Maydis
Kind；And described fermentation raw material crude protein content in terms of butt is more than 20 weight %；Described composite bacteria
Comprise lactobacillus, bacillus cereus and yeast.
According to the second aspect of the invention, the invention provides a kind of fermentative feedstuff of microbe raw material, wherein,
This fermentative feedstuff of microbe raw material is prepared by above-mentioned preparation method.
According to the third aspect of the invention we, present invention also offers a kind of feedstuff, wherein, this feedstuff contains
Fermentative feedstuff of microbe raw material prepared by above-mentioned preparation method.
By technique scheme, the fermentative feedstuff of microbe method for preparing raw material that the application provides utilizes work
Industry by-product, as fermentation raw material, makes high-energy feed or high-protein feed raw material, enters side-product
Row organic combination, turn waste into wealth, make product economy maximizing the benefits；Composite bacteria is used to carry out microorganism
Fermentation, improves the nutritional labeling in feedstuff and absorbance, the nutritive value of side-product can be greatly improved；
It addition, this preparation method production technology is simple, with low cost, fabrication cycle is short, it is simple to large-scale production.
Other features and advantages of the present invention will be described in detail in detailed description of the invention part subsequently.
Detailed description of the invention
Hereinafter the detailed description of the invention of the present invention is described in detail.It should be appreciated that this place is retouched
The detailed description of the invention stated is merely to illustrate and explains the present invention, is not limited to the present invention.
The preparation method of the fermentative feedstuff of microbe raw material that the present invention provides, the method includes composite bacteria
Being inoculated in fermentation raw material and ferment, described fermentation raw material contains corn starch residue, beet molasses, jade
In rice wine essence grain soluble substance, citric acid grain, Semen Maydis powder, distiller's dried grain and soluble substance thereof and whitewashing skin of Semen Maydis
One or more；And described fermentation raw material crude protein content in terms of butt is more than 20 weight %；Described
Composite bacteria comprises lactobacillus, bacillus cereus and yeast.
In the present invention, described fermentation raw material crude protein in terms of butt refers to " in GB/T 6432 feedstuff
Crude protein determining method " data of gained.
According to the present invention, by making described fermentation raw material contain corn starch residue, beet molasses, corn wine
One or more in essence grain soluble substance, citric acid grain, Semen Maydis powder, DDGS and whitewashing skin of Semen Maydis, come
Prepare fermentative feedstuff of microbe raw material.
As above-mentioned corn starch residue, maize alcohol lees soluble substance, citric acid grain, Semen Maydis powder, DDGS
The citric acid that can be known in the art with whitewashing skin of Semen Maydis, lysine, the by-product of the product such as ethanol.
Such as, above-mentioned corn starch residue preferably Semen Maydis is size-reduced, liquefy, filter and the corn starch residue that obtains；
The beet molasses that above-mentioned beet molasses obtains preferably by Radix Betae liquefaction, saccharifying and concentration；Above-mentioned jade
Rice wine essence grain soluble substance preferably Semen Maydis is size-reduced, liquefaction, saccharifying, ferment, distill, filter and obtain
Maize alcohol lees soluble substance；Above-mentioned citric acid grain is preferably with Semen Maydis through liquefaction, saccharifying, fermentation, mistake
The citric acid grain filtered and obtain；The Semen Maydis powder that above-mentioned Semen Maydis powder preferably Semen Maydis is grinding to obtain；Above-mentioned
DDGS preferably Semen Maydis is size-reduced, liquefaction, saccharifying, ferment, filter after obtain liquid and solid slag, dense
The DDGS that contracting obtains with drying；Above-mentioned whitewashing skin of Semen Maydis is preferably and corn steep liquor is sprayed onto skin of Semen Maydis
Upper and drying and the whitewashing skin of Semen Maydis that obtains.Above-mentioned corn starch residue, beet molasses, maize alcohol lees
Soluble substance, citric acid grain, Semen Maydis powder, DDGS and the preparation method of whitewashing skin of Semen Maydis and condition are ability
Well known to territory, it is not repeated at this.
One according to the present invention preferred embodiment, in described corn starch residue in terms of butt crude protein
For 25-30 weight %, in terms of butt, cinder is divided into 2.7-3.3 weight %, counts crude fibre as 9.2-11.2 with butt
Weight %, counts crude fat for 10.8-13.2 weight % with butt, and moisture is 9-11 weight %；
Described maize alcohol lees soluble substance counts crude protein for 14-17 weight % with butt, cinder in terms of butt
Being divided into 11.7-14 weight %, count crude fibre for 0.4-0.6 weight % with butt, in terms of butt, crude fat is
0.12-0.14 weight %, moisture is 60-75 weight %；
Described citric acid grain counts crude protein for 10-13 weight % with butt, and in terms of butt, cinder is divided into
2.7-3.4 weight %, counts crude fibre for 20.5-25 weight % with butt, counts crude fat as 2.3-2.8 with butt
Weight %, moisture is 60-75 weight %；
Described Semen Maydis powder counts crude protein for 7.7-9.4 weight % with butt, and in terms of butt, cinder is divided into 1.2-1.5
Weight %, counts crude fibre for 2.7-3.4 weight % with butt, counts crude fat for 3-3.7 weight % with butt,
Moisture is 12-15 weight %；
Described DDGS counts crude protein for 23-29 weight % with butt, and in terms of butt, cinder is divided into 4.5-5.5
Weight %, counts crude fibre for 10-12 weight % with butt, counts crude fat for 6.6-8.0 weight % with butt,
Moisture is 5.5-12.0 weight %；
Described whitewashing skin of Semen Maydis counts crude protein for 17-21 weight % with butt, and in terms of butt, cinder is divided into 5-6
Weight %, counts crude fibre for 7.4-9 weight % with butt, counts crude fat for 1.9-2.3 weight % with butt,
Moisture is 7.8-12.0 weight %.
Preferably, described fermentation raw material contains Semen Maydis powder, corn starch residue, citric acid grain, corn alcohol
One or more in grain soluble substance, DDGS and whitewashing skin of Semen Maydis.
It is highly preferred that described fermentation raw material be corn starch residue, beet molasses, maize alcohol lees soluble substance,
Citric acid grain, Semen Maydis powder, DDGS and whitewashing skin of Semen Maydis.
As long as additionally, described fermentation raw material in terms of butt crude protein content more than 20 weight %,
From raw material color and luster and granularity, nutritional labeling in terms of consider, more preferably 22-24 weight %.
According to the present invention, for the consideration of nutritional labeling, raw material color and luster and granularity, described fermentation raw material
In terms of butt, crude protein content is on the basis of meeting above-mentioned condition, the moisture in the most described fermentation raw material
Content is below 35 weight % (more preferably 33-34.5 weight %)；It is highly preferred that described fermentation raw material
In terms of butt, crude fibre is below 10 weight % (more preferably 6-8 weight %), crude fat in terms of butt
Being more than 2 weight % (more preferably 3-4 weight %), in terms of butt, cinder is divided into below 10 weight %
(more preferably 6-8 weight %).Here, crude fibre refers to " in GB/T 6434 feedstuff in terms of butt
Crude fibre assay method " measure the data of gained, in like manner, in terms of butt, crude fat refers to " GB/T 6433
The mensuration of crude fat in feedstuff " measure gained data.In terms of butt, coarse ash refers to " GB/T
The mensuration of coarse ash in 6438-2007 feedstuff " measure gained data.
According to the present invention, described composite bacteria comprises lactobacillus, bacillus cereus and yeast.
Preferably, described lactobacillus be bacillus acidophilus's (such as strain of numbered CGMCC 1.1854),
Lactobacillus plantarum (such as the strain of numbered CGMCC 1.2158) and Lactobacillus fermenti are (as numbered
The strain of CGMCC 1.3223) in one or more, described bacillus cereus be bacillus subtilis (as
The strain of numbered Agricultural University Of Anhui Life Science College 951NA4), Bacillus licheniformis (as compile
Number be the strain of CGMCC 1.265) and Bacillus coagulans (such as numbered CGMCC 1.3220
Strain) in one or more, described yeast is that Candida utilis is (such as numbered CGMCC
The strain of 2.1180) and/or saccharomyces cerevisiae (such as the strain of numbered CGMCC 2.3853).
It is highly preferred that described composite bacteria comprises bacillus acidophilus, bacillus subtilis and Candida utilis ferment
Female bacterium.
One of the present invention preferred embodiment in, described composite bacteria by bacillus cereus seed liquor,
Lactobacillus seed liquor and yeast seed liquor mix.Described bacillus cereus seed liquor, lactobacillus seed
Liquid and yeast seed liquor cultivated through shake-flask seed by bacillus cereus, lactobacillus and yeast respectively, one
Level seed culture two step incubation completes.Cultural method and condition are this area usual manner, meet
Strain content needed for the application.
The method cultivated as above-mentioned shake-flask seed can be such as: bacteria suspension accesses sterilized shaking flask
In culture medium, according to strain character, cultivate under the conditions of standing (anaerobism)/bottle swingging machine (aerobic), training
The process of supporting measures OD (wavelength 600nm, cuvette width 1cm) and pH value.
The method cultivated as above-mentioned first order seed can be such as: shake flask culture is accessed first order seed
Culture medium, in the tank that spreads cultivation, cultivates under felicity condition；Incubation mensuration OD (wavelength 600nm,
Cuvette width 1cm) and pH value.
Preferably, the content of described bacillus cereus seed liquor bacillus cereus is 5 × 108～1 × 109Cfu/mL,
In lactobacillus seed liquor, the content of lactobacillus is 5 × 109～1 × 1010Cfu/mL, ferment in yeast seed liquor
The content of female bacterium is 2.5 × 109～5 × 109cfu/mL；
It is highly preferred that described bacillus cereus seed liquor and described lactobacillus seed liquor and described yeast seed
The volume ratio of liquid is 1:1-1.5:0.5-1.
According to the present invention, the condition of described fermentation includes: fermentation temperature be 25-35 DEG C (preferably
28-32 DEG C), fermentation time is 48-168 hour (preferably 72-144 hour), and fermentation pH value is 5-7
(preferably 5.5-6.5).The present inventor finds through in-depth study, under these conditions,
Particularly ferment in above-mentioned pH value range, it is possible to make lactobacillus, bacillus cereus and yeast equal
It is obtained in that fabulous ferment effect, finally gives that to have the fermentative feedstuff of microbe of higher strain content former
Material.As the material of regulation pH value, there is no particular limitation, it is possible to use commonly used in the art
The various reagent for regulating pH value.Such as can be adjusted with calcium hydroxide.
Preferably, relative to fermentation raw material described in 1g, the inoculum concentration of described bacillus cereus is 2 × 105～4
×106Cfu (preferably 4 × 105～4 × 106Cfu), the inoculum concentration of described lactobacillus is 2 × 107～4 ×
108Cfu (preferably 4 × 107～4 × 108Cfu), described saccharomycetic inoculum concentration is 2 × 107～4 × 108cfu
(preferably 4 × 107～4 × 108cfu)。
According to the present invention, described sweat includes aerobe fermentation and two stages of anaerobic fermentation, and aerobic is sent out
Ferment consumes the oxygen in environment, and oxygen carries out anaerobic fermentation process after being exhausted, according to the characteristic of strain
Utilize one time fermentation process to complete the fermentation of three class strains simultaneously.Preferably, described sweat is impermeable
Carrying out in the packaging bag of gas, described packaging bag is provided with one-way exhaust valve, and gas is merely by described unidirectional
Air bleeding valve is discharged bag in bag.In order to make lactobacillus, bacillus cereus and yeast all be obtained in that
Preferably ferment effect, it is preferable that the fermentation raw material in bag with the volume ratio of the air being sealed in bag is
10:0.5-1, discharges excessive gas and seals.
The present invention also provides for a kind of fermentative feedstuff of microbe raw material obtained by above-mentioned preparation method.
The pH value of the fermentative feedstuff of microbe raw material obtained by the preparation method of the present invention is 3.0-5.0,
It is preferably 3.5-4.5, more preferably 3.8-4.2.By pH value within the above range, can effectively press down
Make the pollution of the mycete caused because water content is higher and pathogenic bacterium etc..
Total acid in fermentative feedstuff of microbe raw material embodies organic acid content therein, by the system of the present invention
The fermentative feedstuff of microbe raw material that Preparation Method obtains, counts total acid for 2.5-4.5 weight % with lactic acid, is preferably
3.0-4.5 weight %, more preferably 4.0-4.5 weight %.By total acid within the above range, there is reduction
Animal gastrointestinal tract pH, improve animal gastrointestinal tract microbiota, improve animal feed intake excellent results.
According to the present invention, the fermentative feedstuff of microbe raw material obtained by the preparation method of the present invention is with butt
Meter crude protein is 16-24 weight %；It is preferably 18-24 weight %；More preferably 22-24 weight %.
According to the present invention, by described in the fermentative feedstuff of microbe raw material that the preparation method of the present invention obtains
Bacillus cereus content is 2 × 105～8 × 106Cfu/g, described lactobacillus content is 2 × 107～8 × 108Cfu/g,
Described yeast content is 2 × 107～8 × 108cfu/g；
Preferably, described bacillus cereus content is 1 × 106～8 × 106Cfu/g, described lactobacillus content is 1
×108～8 × 108Cfu/g, described yeast content is 1 × 108～8 × 108cfu/g；
It is highly preferred that described bacillus cereus content is 4 × 106～8 × 106Cfu/g, described lactobacillus content
It is 4 × 108～8 × 108Cfu/g, described yeast content is 4 × 108～8 × 108cfu/g。
According to the present invention, by moisture in the fermentative feedstuff of microbe raw material that the preparation method of the present invention obtains
Content is preferably 30-50 weight %, more preferably 32-34 weight %.
According to the present invention, Feedstock treating prepared by described fermentative feedstuff of microbe raw material, mix, inoculate,
Pack and sweat are carried out by this area conventional method.Specifically, according to the proportioning of various solid materials,
Use forklift to feed intake in each hopper, use belt to claim to realize weighing of solid material, put into bucket type and carry
The machine of liter；Solid material is delivered to wet stock beater, after breaing up, former blocks of solid by bucket elevator
Wet stock is dispersed as uniform little granule, and wet stock beater can produce continuously；Solid after beater
Raw material, enters mixer.Opening liquid charging stock and strain material inlet valve, liquid charging stock is carried out by proportional quantity
Adding, mix with solid material, strain mixes with fermentation raw material, completes seeded process.Mixing 5-10
After minute, the material in mixer is unloaded in wet feed storehouse, by conveying worm extraction in wet feed storehouse,
It is delivered in packaging bag, sealing of weighing.Move to sweathouse by fork truck under fermentation conditions complete to ferment
Journey.
Further, present invention also offers a kind of feedstuff, this feedstuff contains mentioned microorganism fermented feed
Raw material.Described fermentative feedstuff of microbe raw material is for main as feedstuff of the material such as replacement of corn, bean cake
Composition.Described fermentative feedstuff of microbe raw material is 4-12 weight % at the content of feedstuff.
As the present invention one preferred embodiment, the content of mentioned microorganism fermented feed raw material is
8.36 weight %, it is 48.75 weight % that the kind of other composition and content are respectively as follows: Semen Maydis, bean cake
Being 35.37 weight %, soybean oil is 4.84 weight %, and stone powder is 1.38 weight %, and calcium hydrogen phosphate is 1.30
Weight %.
Hereinafter will be described the present invention by embodiment.In following example, total acid is by changing
Titrimetry records, and moisture is by " GB/T 6435 Moisture in Feed and the survey of other volatile matter content
Determine method " record, in terms of butt, crude protein passes through " crude protein determining method in GB/T 6432 feedstuff "
Recording, in terms of butt, coarse ash is measured by " mensuration of coarse ash in GB/T 6438-2007 feedstuff ",
In terms of butt, crude fibre is measured by " crude fibre assay method in GB/T 6434 feedstuff ", in terms of butt
Crude fat is measured by " mensuration of crude fat in GB/T 6433 feedstuff ".
In following example, cell density is recorded by colony counting method, lactobacillus, yeast and
The plating medium compound method that bacillus cereus uses respectively is as follows:
Lactobacillus plating medium: weigh MRS broth bouillon 55.2g/L, agar 10.14g/L, add
Distilled water constant volume, regulation pH value is 6.2,120 DEG C, and plating medium is made in 20min sterilizing, empty
Use after cultivating 24h.
Yeast plating medium: weigh yeast powder 10g/L, peptone 20g/L, glucose 20g/L,
Agar 14g/L, adds distilled water constant volume, and regulation pH value is 6.0,120 DEG C, and 20min sterilizing is made
Plating medium, uses after the empty 24h of cultivation.
Bacillus cereus plating medium: weigh yeast powder 5g/L, peptone 10g/L, glucose 5g/L,
Sodium chloride 10g/L, agar 20g/L, add distilled water constant volume, and regulation pH value is 7.20,120 DEG C, 20min
Sterilizing, makes plating medium, uses after the empty 24h of cultivation.
Cell density uses colony counting method to measure, and weighs 5.0g material in 45g sterilized water, and placement is shaken
Bed cultivates (temperature 32 DEG C, rotating speed 120rpm/min, time 40min-50min), then takes 0.1ml
Culture fluid shakes up to 0.9ml sterilized water, is diluted to certain gradient, is applied to the flat board training of corresponding strain
Support base, place calorstat 30 DEG C and cultivate 48h.Method of counting: a dilution clump count is 30-300
Individual clump count, then with this dilution factor clump count divided by spread plate used diluent volume (0.1ml), take advantage of
Using extension rate as the thalline of this sample sum.
In following embodiment, corn starch residue is for producing in the corn deep processing product process such as citric acid, beautiful
Size-reduced, the filtering residue of acquisition of liquefying, filter of rice, then the product that drying obtains；
Beet molasses is purchased from Guangxi trade Co., Ltd in Ya Tang romote antiquity；
Maize alcohol lees soluble substance is Semen Maydis, infiltrate, pulverize, liquefy, saccharifying, then through culture propagation,
After ethanol is distilled off, the thing that slips remaining still filters the clear liquid (dry 3%) obtained and concentrates (dense
Dry 35% after contracting) after product；
Citric acid grain is to produce during citric acid product with Semen Maydis for fermenting raw materials, and fermentation liquor filters surplus
Remaining filtering residue product, its moisture is 70%；
Semen Maydis powder is the powder that Semen Maydis is grinding to obtain；
DDGS (Distillers Dried Grains with Solubles, distiller's dried grain and soluble substance thereof) is beautiful
Rice plumule infiltrated, de-, pulverize, liquefy, saccharifying, ferment, filtrate that the process distill obtains and clearly
Liquid mixes；
Whitewashing skin of Semen Maydis is to be sprayed onto on skin of Semen Maydis by corn steep liquor and the product of drying acquisition.
In following embodiment, (Lactobacillus acidophilus, CGMCC number bacillus acidophilus
1.1854), Lactobacillus plantarum (Lactobacillus plantarum, CGMCC numbering 1.2158), send out
Kefir milk bacillus (Lactobacillus fermentum, CGMCC numbering 1.3223), Bacillus licheniformis
(Bacillus licheniformis, CGMCC numbering 1.265), Bacillus coagulans (Bacillus
Coagulans, CGMCC numbering 1.3220), Candida utilis (Candida utilis, CGMCC
Numbering 2.1180), saccharomyces cerevisiae (Saccharomyces cerevisiae, CGMCC numbering 2.3853)
Both from China General Microbiological culture presevation administrative center, bacillus subtilis (Bacillus
Subtilis) purchased from Agricultural University Of Anhui's Life Science College, numbered 951NA4.
Embodiment 1
Choose Candida utilis, bacillus subtilis, three kinds of bacterium of bacillus acidophilus as composite bacteria.
(1) shake-flask seed is cultivated
Shake-flask seed incubation is the following culture medium of preparation, 121 DEG C, 20min high temperature sterilize, cooling
After take bacteria suspension and access in culture medium, cultivate under correspondence controls Parameter Conditions, incubation measures OD
(wavelength 600nm, cuvette width 1cm) and pH value, when reaching terminal parameter, shake-flask seed is trained
Support.
Bacillus acidophilus:
Culture medium: MRS cultured solution of broth 48 grams, in 1000ml water, regulates pH value 6.2 ± 0.1.
Control parameter: 30 DEG C, under static conditions, carry out Anaerobic culturel.
Terminal parameter: OD >=0.250
Bacillus subtilis:
Culture medium: yeast powder 5g, peptone 10g, glucose 5g, sodium chloride 10g, water 1000ml,
With 1mol/L NaOH solution regulation pH to meta-alkalescence.
Control parameter: 35 DEG C, 160r/min.
Terminal parameter: OD >=0.120
Candida utilis:
Culture medium: peptone 19g, glucose 19g, yeast extract 9.5g, with water constant volume 1000ml.
Control parameter: 30 DEG C, 200r/min.
Terminal parameter: OD >=0.320
(2) first order seed is cultivated
First order seed incubation is to prepare supplementary material kind as shown in table 1 and the first order seed of adding proportion
Cultivate based on spreading cultivation in tank, 121 DEG C, 20min high temperature sterilize, after cooling, take the training after shake-flask culture
Support base 100mL and access in primary-seed medium 500L, control training under Parameter Conditions as shown in table 1
Supporting, incubation measures OD (wavelength 600nm, cuvette width 1cm), pH value, cultivates to table
Shown in 1 during terminal parameter, first order seed has been cultivated.
Table 1
Wherein, described Semen Maydis pulp is the jade that Semen Maydis sulfurous acid solution soaks, dilute Semen Maydis pulp obtains after concentrating
Rice & peanut milk, wherein dry is 35 weight %.
Semen Maydis pulp is sub-purchased from Guangxi purchased from COFCO Biochemical (Anhui) Co., Ltd., beet molasses
Sugar trade Co., Ltd in romote antiquity, Carnis Bovis seu Bubali cream, Tween 80 are purchased from China of the Anhui Chuzhou limited public affairs of space reagent material
Department.
Through measuring, the content cultivating the bacillus cereus seed liquor bacillus cereus obtained is 5 × 108Cfu/mL,
In lactobacillus seed liquor, the content of lactobacillus is 5 × 109Cfu/mL, saccharomycetic in yeast seed liquor contains
Amount is 2.5 × 109cfu/mL。
(3) preparation of fermentative feedstuff of microbe raw material
By above-mentioned bacillus cereus seed liquor, lactobacillus seed liquor and yeast seed liquor with weight ratio 2:2:
1 is mixed to get strain mixed liquor.
By solvable to 5 parts by weight Corn starch slags, 2 weight portion beet molassess, 26 parts by weight Corn ethanol grains
Thing, 11 parts by weight Corn powder, 4 parts of citric acid grains, 6 weight portion DDGS and 44 weight portions whitewashing jade
Silverskin mix with 2 weight portion strain mixed liquors after breaing up respectively (inoculum concentration of lactobacillus is 1 ×
108Cfu/g, the inoculum concentration of bacillus cereus is 1 × 106Cfu/g, saccharomycetic inoculum concentration is 1 × 108Cfu/g),
Loading air-locked packaging bag, this packaging bag is provided with one-way exhaust valve, and gas is merely by described unidirectional
Air bleeding valve is discharged bag in bag.The volume ratio of the fermentation raw material in packaging bag and the air being sealed in bag
For 10:1.
Fermentation condition is: fermentation temperature is 30 DEG C, and fermentation time is 120 hours, and fermentation pH value is 5.5.
After having fermented, the moisture measuring gained fermentative feedstuff of microbe raw material is 33.50 weight %,
PH value is 4.2, and in terms of lactic acid, total acid is 4.0 weight %, and bacillus acidophilus's content is 4.1 × 108Cfu/g,
Bacillus subtilis bacterial content is 4.3 × 106Cfu/g, Candida utilis content is 4.0 × 108cfu/g；
In terms of butt, crude protein is 22.56 weight %, and in terms of butt, crude fat is 3.45 weight %, in terms of butt
Cinder is divided into 7.65 weight %, and in terms of butt, crude fibre is 7.86 weight %.
Embodiment 2
Fermentative feedstuff of microbe raw material, except for the difference that, this fermentation raw material is prepared according to the method for embodiment 1
And mass ratio is corn starch residue: beet molasses: maize alcohol lees soluble substance: Semen Maydis powder: whitewashing jade
Silverskin: citric acid grain=11:2:26:4:44:11.Fermentation select strain and inoculum concentration be:
Lactobacillus is Lactobacillus plantarum, and its inoculum concentration is 4 × 108Cfu/g, bacillus cereus is Bacillus coagulans,
Its inoculum concentration is 4 × 106Cfu/g, yeast is saccharomyces cerevisiae, and its inoculum concentration is 4 × 108cfu/g。
Fermentation condition is: fermentation temperature is 28 DEG C, and fermentation time is 72 hours, and fermentation pH value is 6.0
Hour.
After having fermented, the moisture measuring gained fermentative feedstuff of microbe raw material is 34.00 weight %,
PH value is 3.9, and in terms of lactic acid, total acid is 4.4 weight %, and lactobacillus content is 7.1 × 108Cfu/g, spore
Bacillus content is 6.9 × 106Cfu/g, yeast content is 7.9 × 108cfu/g；In terms of butt, crude protein is
23.03 weight %, in terms of butt, crude fat is 3.66 weight %, and in terms of butt, cinder is divided into 7.80 weight %,
In terms of butt, crude fibre is 7.53 weight %.
Embodiment 3
Fermentative feedstuff of microbe raw material, except for the difference that, this fermentation raw material is prepared according to the method for embodiment 1
And mass ratio is corn starch residue: beet molasses: maize alcohol lees soluble substance: Semen Maydis powder: DDGS:
Whitewashing skin of Semen Maydis: citric acid grain=3:2:20:4:8:44:17.
Strain and inoculum concentration thereof that fermentation is selected be: lactobacillus is Lactobacillus fermenti, its inoculum concentration is 2 ×
108Cfu/g, bacillus cereus is Bacillus coagulans, and its inoculum concentration is 2 × 106Cfu/g, yeast is for producing protein
Candidiasis, its inoculum concentration is 2 × 108cfu/g。
Fermentation condition is: fermentation temperature is 32 DEG C, and fermentation time is 144 hours, and fermentation pH value is 6.5.
After having fermented, the moisture measuring gained fermentative feedstuff of microbe raw material is 33.80 weight %,
PH value is 4.1, and in terms of lactic acid, total acid is 4.1 weight %, and lactobacillus content is 5.6 × 108Cfu/g, bud
Spore bacillus content is 4.8 × 106Cfu/g, yeast content is 6.9 × 108cfu/g；Crude protein in terms of butt
Being 23.76 weight %, in terms of butt, crude fat is 3.78 weight %, and in terms of butt, cinder is divided into 7.86 weights
Amount %, in terms of butt, crude fibre is 7.29 weight %.
Embodiment 4
Fermentative feedstuff of microbe raw material, except for the difference that, this fermentation raw material is prepared according to the method for embodiment 1
And mass ratio is corn starch residue: beet molasses: maize alcohol lees soluble substance: DDGS: whitewashing jade
Silverskin: citric acid grain=3:2:20:6:50:17.
Strain and inoculum concentration thereof that fermentation is selected be: lactobacillus is bacillus acidophilus, its inoculum concentration is 2 ×
107Cfu/g, bacillus cereus is Bacillus licheniformis, and its inoculum concentration is 2 × 105Cfu/g, yeast is for making
Brewer yeast bacterium, its inoculum concentration is 2 × 107cfu/g。
Fermentation condition is: fermentation temperature is 26 DEG C, and fermentation time is 48 hours, and fermentation pH value is 5.0
Hour.
After having fermented, the moisture measuring gained fermentative feedstuff of microbe raw material is 32.50 weight %,
PH value is 4.5, and in terms of lactic acid, total acid is 2.6 weight %, and lactobacillus content is 3.2 × 107Cfu/g, bud
Spore bacillus content is 2.8 × 105Cfu/g, yeast content is 3.0 × 107cfu/g；Crude protein in terms of butt
Being 21.35 weight %, in terms of butt, crude fat is 2.86 weight %, and in terms of butt, cinder is divided into 8.59 weights
Amount %, in terms of butt, crude fibre is 8.43 weight %.
Embodiment 5
Fermentative feedstuff of microbe raw material, except for the difference that, this fermentation raw material is prepared according to the method for embodiment 1
And mass ratio is corn starch residue: beet molasses: maize alcohol lees soluble substance: DDGS: whitewashing Semen Maydis
Skin: citric acid grain=3:1:25:7:50:12.Fermentation select strain and inoculum concentration be: breast bar
Bacterium is Lactobacillus plantarum, and its inoculum concentration is 3 × 107Cfu/g, bacillus cereus is bacillus subtilis, and it connects
The amount of kind is 3 × 105Cfu/g, yeast is Candida utilis, and its inoculum concentration is 3 × 107cfu/g。
Fermentation condition is: fermentation temperature is 34 DEG C, and fermentation time is 168 hours, and fermentation pH value is 7.0.
After having fermented, the moisture measuring gained fermentative feedstuff of microbe raw material is 33.0 weight %,
PH value is 4.3, and in terms of lactic acid, total acid is 2.9 weight %, and lactobacillus content is 3.5 × 107Cfu/g, bud
Spore bacillus content is 3.2 × 105Cfu/g, yeast is 3.6 × 107cfu/g；In terms of butt, crude protein is
21.65 weight %, in terms of butt, crude fat is 2.43 weight %, and in terms of butt, cinder is divided into 9.56 weight %,
In terms of butt, crude fibre is 8.64 weight %.
The preferred embodiment of the present invention described in detail above, but, the present invention is not limited to above-mentioned reality
Execute the detail in mode, in the technology concept of the present invention, can be to the technical side of the present invention
Case carries out multiple simple variant, and these simple variant belong to protection scope of the present invention.
It is further to note that each the concrete technology described in above-mentioned detailed description of the invention is special
Levy, in the case of reconcilable, can be combined by any suitable means, in order to avoid need not
The repetition wanted, various possible compound modes are illustrated by the present invention the most separately.
Additionally, combination in any can also be carried out between the various different embodiment of the present invention, as long as its
Without prejudice to the thought of the present invention, it should be considered as content disclosed in this invention equally.
Claims (11)
1. a preparation method for fermentative feedstuff of microbe raw material, the method includes inoculating composite bacteria
Ferment in fermentation raw material, it is characterised in that
Described fermentation raw material contains corn starch residue, beet molasses, maize alcohol lees soluble substance, citric acid
One or more in grain, Semen Maydis powder, distiller's dried grain and soluble substance thereof and whitewashing skin of Semen Maydis；And described fermentation
Raw material crude protein content in terms of butt is more than 20 weight %；
Described composite bacteria comprises lactobacillus, bacillus cereus and yeast.
Preparation method the most according to claim 1, wherein, the moisture in described fermentation raw material contains
Amount is below 35 weight %；Described fermentation raw material crude fibre in terms of butt is below 10 weight %, with dry
Base meter crude fat is more than 2 weight %, and in terms of butt, cinder is divided into below 10 weight %.
Preparation method the most according to claim 1, wherein, described lactobacillus be bacillus acidophilus,
One or more in Lactobacillus plantarum and Lactobacillus fermenti, described bacillus cereus be bacillus subtilis,
One or more in Bacillus licheniformis and Bacillus coagulans, described yeast is Candida utilis
Bacterium and/or saccharomyces cerevisiae.
Preparation method the most according to claim 3, wherein, described composite bacteria is by bacillus cereus
Seed liquor, lactobacillus seed liquor and yeast seed liquor mix；
Preferably, in described bacillus cereus seed liquor, the content of bacillus cereus is 5 × 108～1 ×
109Cfu/mL, in lactobacillus seed liquor, the content of lactobacillus is 5 × 109～1 × 1010Cfu/mL, yeast
In seed liquor, saccharomycetic content is 2.5 × 109～5 × 109cfu/mL；
It is highly preferred that described bacillus cereus seed liquor and described lactobacillus seed liquor and described yeast seed
The mixed volume of liquid is than for 1:1-1.5:0.5-1.
5. according to the preparation method described in any one in claim 1-4, wherein, described fermentation
Condition includes: fermentation temperature is 25-35 DEG C, and fermentation time is 48-168 hour, and fermentation pH value is 5-7；
Preferably, relative to fermentation raw material described in 1g, the inoculum concentration of described bacillus cereus is 2 × 105～4
×106Cfu, the inoculum concentration of described lactobacillus is 2 × 107～4 × 108Cfu, described saccharomycetic inoculum concentration
It is 2 × 107～4 × 108cfu。
6. according to the preparation method described in any one in claim 1-5, wherein, described fermented
Journey is carried out in air-locked packaging bag, and described packaging bag is provided with one-way exhaust valve, gas merely by
Described one-way exhaust valve is discharged bag in bag.
7. the fermentable prepared by the preparation method described in any one in claim 1-6
Feedstuff.
Fermentative feedstuff of microbe raw material the most according to claim 7, wherein, described microorganism is sent out
The pH value of ferment feedstuff is 3-5, and in terms of lactic acid, total acid is more than 2.5 weight %, thick egg in terms of butt
It is more than 16 weight % in vain；
Preferably, the pH value of described fermentative feedstuff of microbe raw material is 3.5-4.5, and in terms of lactic acid, total acid is
More than 3 weight %, in terms of butt, crude protein is more than 18 weight %；
It is highly preferred that the pH value of described fermentative feedstuff of microbe raw material is 3.8-4.2, total acid in terms of lactic acid
Being more than 4 weight %, in terms of butt, crude protein is more than 22 weight %.
9. according to the fermentative feedstuff of microbe raw material described in claim 7 or 8, wherein, described micro-life
Described lactobacillus >=2 × 10 in thing fermented feed raw material7Cfu/g, described bacillus cereus >=2 × 105Cfu/g,
Described yeast >=2 × 107cfu/g；
Preferably, described lactobacillus >=1 × 108Cfu/g, described bacillus cereus >=1 × 106Cfu/g, described
Yeast >=1 × 108cfu/g；
It is highly preferred that described lactobacillus >=4 × 108Cfu/g, described bacillus cereus >=4 × 106Cfu/g, institute
State yeast >=4 × 108cfu/g。
Fermentative feedstuff of microbe raw material the most according to claim 9, wherein, described microorganism is sent out
Moisture in ferment feedstuff is 30-50 weight %, preferably 32-34 weight %.
11. 1 kinds of feedstuffs, it is characterised in that this feedstuff contains any one institute in claim 7-10
The fermentative feedstuff of microbe raw material stated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610183590.5A CN105831394A (en) | 2016-03-25 | 2016-03-25 | Preparation method of microbial fermentation feed material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610183590.5A CN105831394A (en) | 2016-03-25 | 2016-03-25 | Preparation method of microbial fermentation feed material |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105831394A true CN105831394A (en) | 2016-08-10 |
Family
ID=56584796
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610183590.5A Pending CN105831394A (en) | 2016-03-25 | 2016-03-25 | Preparation method of microbial fermentation feed material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105831394A (en) |
Cited By (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106107023A (en) * | 2016-08-26 | 2016-11-16 | 北京斯隆生物技术有限责任公司 | A kind of microbial degradation humic acids feedstuff and preparation method thereof |
| CN106538832A (en) * | 2016-11-07 | 2017-03-29 | 福建省新闽科生物科技开发有限公司 | A kind of improvement manioc waste preparation technology rich in probiotics |
| CN106689671A (en) * | 2016-12-28 | 2017-05-24 | 新昌县赛因斯生物科技有限公司 | Biological bacterium-containing livestock feed and preparation method thereof |
| CN107937306A (en) * | 2017-11-27 | 2018-04-20 | 上海创博生态工程有限公司 | A kind of microbe leaven for fermented liquid feed and preparation method thereof |
| CN108077559A (en) * | 2017-11-28 | 2018-05-29 | 吉林中粮生化有限公司 | A kind of fermentative feedstuff of microbe and preparation method thereof |
| CN108157592A (en) * | 2016-12-07 | 2018-06-15 | 中粮生物化学（安徽）股份有限公司 | A kind of fermentative feedstuff of microbe raw material and its production method and feed |
| CN108157586A (en) * | 2016-12-07 | 2018-06-15 | 中粮生物化学（安徽）股份有限公司 | A kind of fermentative feedstuff of microbe raw material and its production method and feed |
| CN108260708A (en) * | 2016-12-30 | 2018-07-10 | 中粮集团有限公司 | A kind of dedicated nonreactive fermentation compound feed of ruminant and preparation method thereof |
| CN109221623A (en) * | 2018-12-03 | 2019-01-18 | 河南工业大学 | A kind of preparation method and application of feed additive |
| CN109463536A (en) * | 2018-12-11 | 2019-03-15 | 固原宝发农牧有限责任公司 | A kind of fermentative feedstuff of microbe and preparation method thereof |
| CN109588538A (en) * | 2018-12-25 | 2019-04-09 | 浙江皇冠科技有限公司 | A kind of not antibiotic fermentative feedstuff of microbe and preparation method thereof |
| CN109757605A (en) * | 2019-03-15 | 2019-05-17 | 南京工业大学 | Method for producing animal feed by fermenting corn alcohol lees and waste bean dregs |
| CN109984258A (en) * | 2017-12-29 | 2019-07-09 | 中粮生物化学（安徽）股份有限公司 | Animal feed and preparation method thereof |
| CN111227122A (en) * | 2018-11-29 | 2020-06-05 | 中粮营养健康研究院有限公司 | Production method of fiber-containing ruminant feed and feed produced by same |
| CN111345395A (en) * | 2018-12-24 | 2020-06-30 | 中粮营养健康研究院有限公司 | Production method of fiber-containing ruminant fermented feed |
| CN111345396A (en) * | 2018-12-24 | 2020-06-30 | 中粮生物化学（安徽）股份有限公司 | Fermented feed, preparation method and application thereof, and laying hen feed |
| CN112175857A (en) * | 2019-07-05 | 2021-01-05 | 中粮生物化学（安徽）股份有限公司 | Plant lactobacillus solid microbial inoculum and preparation method and application thereof |
| CN112544797A (en) * | 2019-09-10 | 2021-03-26 | 济南强毅化工科技有限公司 | Corn starch residue mixed feed and preparation method thereof |
| CN112806491A (en) * | 2021-01-15 | 2021-05-18 | 浙江省农业科学院 | Fermented feed for Chunhua pigs and preparation method thereof |
| CN113115858A (en) * | 2019-12-31 | 2021-07-16 | 内蒙古工业大学 | Corn bran mycoprotein fermentation feed and preparation method thereof |
| CN113826757A (en) * | 2021-08-12 | 2021-12-24 | 陕西杨凌富仕特生物科技有限公司 | Yeast culture rich in active probiotics, preparation method and special equipment thereof |
| CN114456990A (en) * | 2022-03-25 | 2022-05-10 | 宁夏伊品生物科技股份有限公司 | Preparation method of DDGS (distillers dried grains with soluble) as well as fermentation strain and culture medium thereof |
| CN115024404A (en) * | 2022-05-24 | 2022-09-09 | 贵州省畜牧兽医研究所 | Feed for Guizhou yellow cattle and preparation method thereof |
| CN115606688A (en) * | 2022-09-05 | 2023-01-17 | 新疆天康饲料有限公司 | Fermented wet feed capable of preventing caking and having water content of more than or equal to 40% and application thereof |
| CN117461729A (en) * | 2023-12-07 | 2024-01-30 | 宜宾市南溪区国科中农生物科技有限公司 | Fermented biological feed containing vinasse and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101352197A (en) * | 2007-07-27 | 2009-01-28 | 严明 | Method for producing fermented feed and anaerobic fermentation bag thereof |
-
2016
- 2016-03-25 CN CN201610183590.5A patent/CN105831394A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101352197A (en) * | 2007-07-27 | 2009-01-28 | 严明 | Method for producing fermented feed and anaerobic fermentation bag thereof |
Cited By (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106107023A (en) * | 2016-08-26 | 2016-11-16 | 北京斯隆生物技术有限责任公司 | A kind of microbial degradation humic acids feedstuff and preparation method thereof |
| CN106538832A (en) * | 2016-11-07 | 2017-03-29 | 福建省新闽科生物科技开发有限公司 | A kind of improvement manioc waste preparation technology rich in probiotics |
| CN108157592A (en) * | 2016-12-07 | 2018-06-15 | 中粮生物化学（安徽）股份有限公司 | A kind of fermentative feedstuff of microbe raw material and its production method and feed |
| CN108157586A (en) * | 2016-12-07 | 2018-06-15 | 中粮生物化学（安徽）股份有限公司 | A kind of fermentative feedstuff of microbe raw material and its production method and feed |
| CN106689671A (en) * | 2016-12-28 | 2017-05-24 | 新昌县赛因斯生物科技有限公司 | Biological bacterium-containing livestock feed and preparation method thereof |
| CN108260708A (en) * | 2016-12-30 | 2018-07-10 | 中粮集团有限公司 | A kind of dedicated nonreactive fermentation compound feed of ruminant and preparation method thereof |
| CN107937306A (en) * | 2017-11-27 | 2018-04-20 | 上海创博生态工程有限公司 | A kind of microbe leaven for fermented liquid feed and preparation method thereof |
| CN108077559A (en) * | 2017-11-28 | 2018-05-29 | 吉林中粮生化有限公司 | A kind of fermentative feedstuff of microbe and preparation method thereof |
| CN109984258A (en) * | 2017-12-29 | 2019-07-09 | 中粮生物化学（安徽）股份有限公司 | Animal feed and preparation method thereof |
| CN111227122A (en) * | 2018-11-29 | 2020-06-05 | 中粮营养健康研究院有限公司 | Production method of fiber-containing ruminant feed and feed produced by same |
| CN109221623A (en) * | 2018-12-03 | 2019-01-18 | 河南工业大学 | A kind of preparation method and application of feed additive |
| CN109463536A (en) * | 2018-12-11 | 2019-03-15 | 固原宝发农牧有限责任公司 | A kind of fermentative feedstuff of microbe and preparation method thereof |
| CN111345395A (en) * | 2018-12-24 | 2020-06-30 | 中粮营养健康研究院有限公司 | Production method of fiber-containing ruminant fermented feed |
| CN111345396A (en) * | 2018-12-24 | 2020-06-30 | 中粮生物化学（安徽）股份有限公司 | Fermented feed, preparation method and application thereof, and laying hen feed |
| CN109588538A (en) * | 2018-12-25 | 2019-04-09 | 浙江皇冠科技有限公司 | A kind of not antibiotic fermentative feedstuff of microbe and preparation method thereof |
| CN109757605A (en) * | 2019-03-15 | 2019-05-17 | 南京工业大学 | Method for producing animal feed by fermenting corn alcohol lees and waste bean dregs |
| CN109757605B (en) * | 2019-03-15 | 2022-01-14 | 南京工业大学 | Method for producing animal feed by fermenting corn alcohol lees and waste bean dregs |
| CN112175857A (en) * | 2019-07-05 | 2021-01-05 | 中粮生物化学（安徽）股份有限公司 | Plant lactobacillus solid microbial inoculum and preparation method and application thereof |
| CN112544797A (en) * | 2019-09-10 | 2021-03-26 | 济南强毅化工科技有限公司 | Corn starch residue mixed feed and preparation method thereof |
| CN113115858A (en) * | 2019-12-31 | 2021-07-16 | 内蒙古工业大学 | Corn bran mycoprotein fermentation feed and preparation method thereof |
| CN112806491A (en) * | 2021-01-15 | 2021-05-18 | 浙江省农业科学院 | Fermented feed for Chunhua pigs and preparation method thereof |
| CN113826757A (en) * | 2021-08-12 | 2021-12-24 | 陕西杨凌富仕特生物科技有限公司 | Yeast culture rich in active probiotics, preparation method and special equipment thereof |
| CN113826757B (en) * | 2021-08-12 | 2024-03-26 | 陕西杨凌富仕特生物科技有限公司 | Yeast culture rich in active probiotics, preparation method and special equipment thereof |
| CN114456990A (en) * | 2022-03-25 | 2022-05-10 | 宁夏伊品生物科技股份有限公司 | Preparation method of DDGS (distillers dried grains with soluble) as well as fermentation strain and culture medium thereof |
| CN114456990B (en) * | 2022-03-25 | 2024-02-09 | 宁夏伊品生物科技股份有限公司 | Preparation method of DDGS and fermentation strain and culture medium thereof |
| CN115024404A (en) * | 2022-05-24 | 2022-09-09 | 贵州省畜牧兽医研究所 | Feed for Guizhou yellow cattle and preparation method thereof |
| CN115606688A (en) * | 2022-09-05 | 2023-01-17 | 新疆天康饲料有限公司 | Fermented wet feed capable of preventing caking and having water content of more than or equal to 40% and application thereof |
| CN117461729A (en) * | 2023-12-07 | 2024-01-30 | 宜宾市南溪区国科中农生物科技有限公司 | Fermented biological feed containing vinasse and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105831394A (en) | Preparation method of microbial fermentation feed material | |
| CN103798503B (en) | The ruminant Tiny ecosystem functional feed of composite zymocyte liquid and preparation method | |
| CN111363698B (en) | Microbial inoculum for reducing mildew and mycotoxin harm of fermented feed and application | |
| CN103232272B (en) | A kind of compound organic microbial fertilizer and manufacture method thereof | |
| CN101690540B (en) | Method for preparing composite protein feed by mixed fermentation | |
| CN104212746B (en) | A kind of changing food waste of salt tolerant processes composite bacteria agent and its preparation method and application | |
| CN101109008A (en) | Aquifer amendment containing multiple active microorganisms and method of preparing the same | |
| CN104000006B (en) | A kind of three stage mixed culture solid state fermentations prepare the method for vinasse protein feed | |
| CN101294141A (en) | A set of living body microorganism preparations for preparing composite microorganism fertilizer and preparation method thereof | |
| CN103911323A (en) | Bacillus licheniformis, bacillus subtilis and lactobacillus plantarum preparation and preparation | |
| CN102687792A (en) | Feeding microecologic preparation based on beer grains and rice bran meal | |
| CN108208335A (en) | A kind of technique for improving cotton dregs Forage Nutritive Value using microbe fermentation method | |
| CN103087964A (en) | Bacillus subtilis, microecological preparation thereof and application of bacillus subtilis in animal feed | |
| CN103184174B (en) | Production method of bacillus subtilis biological agent used for sodium humate-containing feed in medium | |
| CN103820339B (en) | A kind of dehydrated solid-state combination microbial inoculum improving manioc waste protein level and preparation method thereof | |
| CN102234623A (en) | Microbial preparation and fermentation process thereof | |
| CN105543131A (en) | Compound bacteria/cottonseed meal fermented feed and preparation method thereof | |
| CN106561996A (en) | Method for producing feed through enzyme-linked micro-ecologic solid state fermentation of vinasse | |
| CN102113629A (en) | Method for preparing dairy cow feed by microbial fermentation | |
| CN103749987B (en) | A kind of coarse cereals are mixed dregs of rice daily ration enzyme preparation and preparation method thereof | |
| CN108157592A (en) | A kind of fermentative feedstuff of microbe raw material and its production method and feed | |
| CN101407762A (en) | Microbial solid inocula, and preparation and use thereof | |
| CN101194668A (en) | Process for preparing blood meal biological modified peptide protein and application of the same | |
| CN109423466B (en) | Compound fermentation inoculant and application thereof | |
| CN105861615A (en) | Production method of amino acid bioactive peptide for aquatic products |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160810 |