CN108157586A - A kind of fermentative feedstuff of microbe raw material and its production method and feed - Google Patents
A kind of fermentative feedstuff of microbe raw material and its production method and feed Download PDFInfo
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- CN108157586A CN108157586A CN201611115945.3A CN201611115945A CN108157586A CN 108157586 A CN108157586 A CN 108157586A CN 201611115945 A CN201611115945 A CN 201611115945A CN 108157586 A CN108157586 A CN 108157586A
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- 239000002994 raw material Substances 0.000 title claims abstract description 95
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 26
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 43
- 239000002253 acid Substances 0.000 claims abstract description 33
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 31
- 241000186660 Lactobacillus Species 0.000 claims abstract description 28
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 28
- 235000019784 crude fat Nutrition 0.000 claims abstract description 21
- 235000019750 Crude protein Nutrition 0.000 claims abstract description 20
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000004310 lactic acid Substances 0.000 claims abstract description 7
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 7
- 238000000855 fermentation Methods 0.000 claims description 64
- 230000004151 fermentation Effects 0.000 claims description 61
- 240000008042 Zea mays Species 0.000 claims description 59
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 59
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 51
- 230000004913 activation Effects 0.000 claims description 40
- 241000894006 Bacteria Species 0.000 claims description 39
- 244000063299 Bacillus subtilis Species 0.000 claims description 32
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 32
- 241000235646 Cyberlindnera jadinii Species 0.000 claims description 31
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 31
- 235000005822 corn Nutrition 0.000 claims description 31
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 28
- 235000009973 maize Nutrition 0.000 claims description 28
- 235000013339 cereals Nutrition 0.000 claims description 21
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 19
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 19
- 239000002054 inoculum Substances 0.000 claims description 19
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 19
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 18
- 229920002261 Corn starch Polymers 0.000 claims description 15
- 239000008120 corn starch Substances 0.000 claims description 15
- 235000013312 flour Nutrition 0.000 claims description 13
- 239000001301 oxygen Substances 0.000 claims description 13
- 229910052760 oxygen Inorganic materials 0.000 claims description 13
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 12
- 239000007789 gas Substances 0.000 claims description 10
- 238000004806 packaging method and process Methods 0.000 claims description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 241000193749 Bacillus coagulans Species 0.000 claims description 4
- 241000194108 Bacillus licheniformis Species 0.000 claims description 4
- 241000186840 Lactobacillus fermentum Species 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 229940054340 bacillus coagulans Drugs 0.000 claims description 4
- 230000000813 microbial effect Effects 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- 102000004169 proteins and genes Human genes 0.000 claims description 4
- 235000012424 soybean oil Nutrition 0.000 claims description 4
- 239000003549 soybean oil Substances 0.000 claims description 4
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 3
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 3
- 210000000481 breast Anatomy 0.000 claims description 3
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 3
- 241000222122 Candida albicans Species 0.000 claims description 2
- 206010007134 Candida infections Diseases 0.000 claims description 2
- 201000003984 candidiasis Diseases 0.000 claims description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims 2
- 241000196324 Embryophyta Species 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 22
- 239000007788 liquid Substances 0.000 abstract description 10
- 238000005516 engineering process Methods 0.000 abstract description 4
- 229940099112 cornstarch Drugs 0.000 description 14
- 239000000835 fiber Substances 0.000 description 14
- 239000000047 product Substances 0.000 description 13
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 11
- 230000000052 comparative effect Effects 0.000 description 8
- 235000013399 edible fruits Nutrition 0.000 description 8
- 239000008103 glucose Substances 0.000 description 7
- 244000005700 microbiome Species 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 6
- 239000003818 cinder Substances 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 229920002472 Starch Polymers 0.000 description 5
- 239000004615 ingredient Substances 0.000 description 5
- 230000004060 metabolic process Effects 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 239000000843 powder Substances 0.000 description 5
- 239000002893 slag Substances 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 239000008107 starch Substances 0.000 description 5
- 235000019698 starch Nutrition 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 238000003556 assay Methods 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 239000006227 byproduct Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000002699 waste material Substances 0.000 description 4
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000002801 charged material Substances 0.000 description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- 230000014759 maintenance of location Effects 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 235000005979 Citrus limon Nutrition 0.000 description 2
- 244000131522 Citrus pyriformis Species 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 241000726221 Gemma Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 238000010564 aerobic fermentation Methods 0.000 description 2
- 235000019789 appetite Nutrition 0.000 description 2
- 230000036528 appetite Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 235000019197 fats Nutrition 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- SPFMQWBKVUQXJV-BTVCFUMJSA-N (2r,3s,4r,5r)-2,3,4,5,6-pentahydroxyhexanal;hydrate Chemical compound O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O SPFMQWBKVUQXJV-BTVCFUMJSA-N 0.000 description 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 208000019901 Anxiety disease Diseases 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- 240000003183 Manihot esculenta Species 0.000 description 1
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000036506 anxiety Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- FUFJGUQYACFECW-UHFFFAOYSA-L calcium hydrogenphosphate Chemical compound [Ca+2].OP([O-])([O-])=O FUFJGUQYACFECW-UHFFFAOYSA-L 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 235000019700 dicalcium phosphate Nutrition 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- -1 unlike Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The present invention relates to field of fodder, a kind of fermentative feedstuff of microbe raw material and its production method and feed are disclosed, in the fermentative feedstuff of microbe raw material, the content 2 × 10 of bacillus8‑6×108Cfu/g, the content 5 × 10 of saccharomycete6‑7×106Cfu/g, the content 2 × 10 of lactobacillus8‑6×108Cfu/g, total acid content is counted as 46 weight % using lactic acid, the coarse-fibred 89 weight % of content in terms of butt, the 22 23 weight % of content of crude protein in terms of butt, the 3 3.2 weight % of content of crude fat in terms of butt, the 8.7 9 weight % of content of coarse ash in terms of butt, the 14.9 16 weight % of content of the molten albumen of acid in terms of butt.Fermentative feedstuff of microbe raw material nutriment prepared by the method for the invention enriches and avoids the complicated technology using liquid seed liquor.
Description
Technical field
The present invention relates to field of fodder, and in particular, to a kind of fermentative feedstuff of microbe raw material and its production method and feeding
Material.
Background technology
Using corn, manioc waste as raw material, during production citric acid, lysine, alcohol product, there are many meeting Cheng Fuyu
Class byproduct, such as citric acid grain, corn starch residue, corn pulp, maize peel.When byproduct is individually handled or is sold, market
Value does not obtain best embodiment.Microbial fermentation is carried out using mixed raw material, composite bacteria, makes high-energy feed or height
Protein feed raw material carries out company's byproduct organic combination, turns waste into wealth, can make product maximizing the benefits.
Therefore, Applied Biotechnology is increasingly taken seriously in feed industry, because it can turn waste into wealth, adequately
Using trade waste, abundant feed resource had not only been provided in this way, but also alleviate environmental pollution.Biological feedstuff is contained due to it
The advantage of the albumen of high-content and abundant bioactive substance, to alleviate feedstuff anxiety situation, to feed industry with
Huge impetus is played in the development of animal husbandry.
With popularizing for fermented feed application range, the promotion of fermented feed yield is extremely urgent, and liquid seed liquor
Preparation is to restrict a key factor of yield：Liquid seed liquid preparing process is complicated, retention cycle is short.Therefore, by solid bacterium
Agent be applied to fermented feed, be a breakthrough progress, the limitation in the directions such as equipment, technology, manpower can be solved, be stablize and
An important factor for Improving The Quality of Products and raising yield.
Invention content
Present invention aim to address using in the prior art, the production technology of seed liquor production fermented feed is complicated, preserves
The defects of period is short provides a kind of fermentative feedstuff of microbe raw material and its production method and feed.
To achieve these goals, the present invention provides a kind of fermentative feedstuff of microbe raw material, wherein, the microorganism hair
In ferment feedstuff, the content 2 × 10 of bacillus8-6×108Cfu/g, the content 5 × 10 of saccharomycete6-7×106Cfu/g,
The content 2 × 10 of lactobacillus8-6×108Cfu/g counts total acid content as 4-6 weight % using lactic acid, coarse-fibred in terms of butt to contain
8-9 weight %, the content 22-23 weight % of crude protein in terms of butt are measured, the content 3.2-3.4 weights of crude fat in terms of butt
%, the content 8.8-9.2 weight % of coarse ash in terms of butt are measured, the content 15.5-16.5 weight % of the molten albumen of acid in terms of butt.
The present invention also provides a kind of production method of fermentative feedstuff of microbe raw material, wherein, which includes：It is living
Change bacillus and be added to the step of activated yeast and by the saccharomycete of lactobacillus, the bacillus of activation and activation
The step of being fermented in fermentation raw material；
Activation bacillus mode be：Under conditions of 20-40 DEG C, bacillus is placed in concentration 1-3 weight %'s
20-40min in glucose solution；
The mode of activated yeast is：Under conditions of 20-40 DEG C, saccharomycete is placed in water 20-40min；
The fermentation raw material contain corn starch residue, maize alcohol lees soluble matter, citric acid grain, corn flour, distiller's dried grain and
It is one or more in its soluble matter and whitewashing maize peel, and the fermentation raw material counts crude protein content as 20 weight % using butt
More than.
The present invention also provides a kind of fermentative feedstuff of microbe raw materials prepared by production method of the present invention.
The present invention also provides a kind of feed, wherein, which contains fermentative feedstuff of microbe raw material of the present invention
And soybean oil.
The method of the invention is will to directly apply in fermentation raw material to send out after solid fungicide progress Selective activation
Ferment, complex process when avoiding using liquid seed liquor, the defects of retention cycle is short.The method of the invention also has following
The advantage of three aspects：
(1) for feedstuff in itself, during feed fermentation and storage, the enzyme system that micro-organisms metabolism generates can make
Fiber, starch, protein degradation are the small-molecule substance for being more easy to be absorbed and utilized by animal, improve conversion rate and the conversion of feed
Rate, this is also the key factor for improving production performance, ensuring product safety quality；
(2) probiotics quantity is improved, adjusts gastrolintestinal microecology balance, inhibits the proliferation of enteron aisle undesirable microorganism, keeps intestines
The health in road；
(3) growth metabolism of microorganism makes to produce for improving containing abundant microbial metabolic products in fermented feed
Performance plays an important role.
Other features and advantages of the present invention will be described in detail in subsequent specific embodiment part.
Specific embodiment
The specific embodiment of the present invention is described in detail below.It is it should be understood that described herein specific
Embodiment is merely to illustrate and explain the present invention, and is not intended to restrict the invention.
The endpoint of disclosed range and any value are not limited to the accurate range or value herein, these ranges or
Value should be understood to comprising the value close to these ranges or value.For numberical range, between the endpoint value of each range, respectively
It between the endpoint value of a range and individual point value and can be individually combined with each other between point value and obtain one or more
New numberical range, these numberical ranges should be considered as specific open herein.
The present invention provides a kind of fermentative feedstuff of microbe raw material, wherein, in the fermentative feedstuff of microbe raw material, gemma
The content 2 × 10 of bacillus8-6×108Cfu/g, the content 5 × 10 of saccharomycete6-7×106Cfu/g, the content 2 × 10 of lactobacillus8-
6×108Cfu/g counts total acid content as 4-6 weight %, the coarse-fibred content 8-9 weight % in terms of butt, with butt using lactic acid
Count the content 22-23 weight % of crude protein, the content 3.2-3.4 weight % of crude fat, the coarse ash in terms of butt in terms of butt
Content 8.8-9.2 weight %, the content 15.5-16.5 weight % of the molten albumen of acid in terms of butt.The thalline being referred to herein contains
It measures (or inoculum concentration) and presses viable bacteria gauge.In the present invention, the content of certain ingredient in terms of butt refers to fermentative feedstuff of microbe
The content of certain ingredient calculated on the basis of the dry weight of raw material.
It is described especially under conditions of the content of total acid in terms of lactic acid under conditions of above-mentioned each component content range
The pH value of fermentative feedstuff of microbe raw material is 3-5.
According to the present invention, animal is liked with acid is fragrant, food of mellowness, in order to stimulate animal appetite, improve feed intake and
Production performance, under preferable case, in the fermentative feedstuff of microbe raw material, the content 4 × 10 of bacillus8-5.5×108cfu/
G, the content 6 × 10 of saccharomycete6-7×106Cfu/g, the content 2.5 × 10 of lactobacillus8-6×108Cfu/g, the total acid in terms of lactic acid
Content is 5-6 weight %, the coarse-fibred content 8.3-8.6 weight % in terms of butt, the content 22.6-23 of crude protein in terms of butt
Weight %, the content 3.21-3.4 weight % of crude fat in terms of butt, the content 8.85-9.2 weight % of coarse ash in terms of butt,
The content 16.2-16.5 weight % of the molten albumen of acid in terms of butt；
In the present invention, with the total weight of the fermentative feedstuff of microbe raw material, the fermentative feedstuff of microbe raw material is also
It can the moisture containing 30-45 weight %, preferably 30-40 weight %.
In the present invention, in the fermentative feedstuff of microbe raw material can also contain starch, minerals and inevitably it is miscellaneous
Matter.On the basis of the dry weight of the fermentative feedstuff of microbe raw material, starch, minerals and inevitable impurity always contain
Amount can be 55.8-57 weight %.
There is no particular limitation for selection of the present invention to the lactobacillus, bacillus and saccharomycete, can be this field
Conventional selection, for example, can the lactobacillus can be one kind in lactobacillus acidophilus, lactobacillus plantarum and lactobacillus fermenti
Or it is a variety of, the bacillus can be one kind or more in bacillus subtilis, bacillus licheniformis and bacillus coagulans
Kind, the saccharomycete can be candida utili bacterium and/or S. cervisiae.
It is further preferred that the lactobacillus be lactobacillus acidophilus, the bacillus be bacillus subtilis, the ferment
Female bacterium is candida utili bacterium.
The present invention also provides a kind of production method of fermentative feedstuff of microbe raw material, wherein, which includes：It is living
Change bacillus and be added to the step of activated yeast and by the saccharomycete of lactobacillus, the bacillus of activation and activation
The step of being fermented in fermentation raw material；
Activation bacillus mode be：Under conditions of 20-40 DEG C, bacillus is placed in concentration 1-3 weight %'s
20-40min in glucose solution；
The mode of activated yeast is：Under conditions of 20-40 DEG C, saccharomycete is placed in water 20-40min；
The fermentation raw material contain corn starch residue, maize alcohol lees soluble matter, citric acid grain, corn flour, distiller's dried grain and
It is one or more in its soluble matter (DDGS) and whitewashing maize peel, and the fermentation raw material counts crude protein content as 20 using butt
More than weight %.
Under these conditions, particularly under the activation condition of above-mentioned bacillus and saccharomycete, activation bacillus and
During fermentation and storage, the enzyme system that micro-organisms metabolism generates can make fiber, starch, egg for activated yeast and lactobacillus
White matter is degraded to the small-molecule substance for being more easy to be absorbed and utilized by animal, also, improves probiotics quantity, adjusts gastrolintestinal microecology and puts down
Weighing apparatus inhibits the proliferation of enteron aisle undesirable microorganism, keeps the health of enteron aisle.Preferably, for activating the glucose water of bacillus
A concentration of 2 weight % of solution.
According to the present invention, to the inoculum concentration of the bacillus, lactobacillus and saccharomycete, there is no particular limitation, preferably feelings
Under condition, relative to every gram of fermentation raw material, the inoculum concentration of the bacillus is 1 × 107-1×108Cfu, more preferably 5 ×
107-1×108cfu；The inoculum concentration of the lactobacillus is 1 × 106-5×107Cfu, more preferably 1 × 107-5×107cfu；Institute
The inoculum concentration for stating saccharomycete is 1 × 105-2×106Cfu, more preferably 1 × 106-2×106cfu." inoculum concentration " refers to
The amount of viable bacteria.
There is no particular limitation for selection of the present invention to the bacillus, lactobacillus and saccharomycete, can be this field
Conventional selection.For example, it (is Agricultural University Of Anhui's life science as numbered that the bacillus, which can be bacillus subtilis,
The strain of institute 951NA4), bacillus licheniformis (as number be CGMCC 1.265 strain) and bacillus coagulans (as number
Strain for CGMCC 1.3220) in it is one or more, the saccharomycete can be candida utili bacterium (as number be
The strain of CGMCC2.1180) and/or S. cervisiae (as number be CGMCC 2.3853 strain), the lactobacillus can be with
Be lactobacillus plantarum (as number be CGMCC 1.2158 strain), lactobacillus acidophilus (as number be CGMCC 1.1854 bacterium
Kind) and lactobacillus fermenti (as number be CGMCC 1.3223 strain) in it is one or more,.
A preferred embodiment of the invention, the lactobacillus are lactobacillus acidophilus, and the bacillus is withered
Careless bacillus, the saccharomycete are candida utili bacterium.
Corn starch residue, maize alcohol lees soluble matter, citric acid grain, corn flour, DDGS and spray in the fermentation raw material
The by-product of the products such as citric acid, lysine, the alcohol that slurry maize peel can be known in the art.For example, the cornstarch
Slag be preferably corn through crush, liquefy, filter obtained from corn starch residue；The maize alcohol lees soluble matter is preferably corn
Through crush, liquefy, be saccharified, ferment, distill, filter obtained from maize alcohol lees soluble matter；The citric acid grain preferably with
Corn through liquefy, be saccharified, ferment, filter obtained from citric acid grain；The corn flour is preferably the jade that corn is grinding to obtain
Rice flour；The DDGS is preferably that corn obtains liquid and solid slag, concentration and drying after crushing, liquefying, be saccharified, ferment, filter
Obtained from DDGS；Corn steep liquor is preferably sprayed onto on maize peel and through whitewashing obtained from drying by the whitewashing maize peel
Maize peel.The corn starch residue, maize alcohol lees soluble matter, citric acid grain, corn flour, DDGS and whitewash maize peel preparation
Method and condition is well known in the art, herein no longer Ao Shu.
According to a preferred embodiment of the present invention, crude protein is counted as 25-30 using butt in the corn starch residue
Weight % counts the content of the molten albumen of acid as 2.5-3.5 weight % using butt, and cinder is divided into 2.7-3.3 weight % in terms of butt, with
Butt meter crude fibre is 9.2-11.2 weight %, and crude fat is counted as 10.8-13.2 weight % using butt, and moisture is 9-11 weights
Measure %；
The maize alcohol lees soluble matter counts crude protein as 14-17 weight % using butt, and sour molten albumen contains in terms of butt
It measures as 16-20 weight %, cinder is divided into 11.7-14 weight % in terms of butt, and crude fibre is counted as 0.4-0.6 weight % using butt,
Crude fat is counted as 0.12-0.14 weight % using butt, moisture is 60-75 weight %；
The citric acid grain counts crude protein as 10-13 weight % using butt, and the content of the molten albumen of acid is counted as 2-3 weights using butt
% is measured, cinder is divided into 2.7-3.4 weight % in terms of butt, counts crude fibre as 20.5-25 weight % using butt, the thick fat in terms of butt
Fat is 2.3-2.8 weight %, and moisture is 60-75 weight %；
The corn flour counts crude protein as 7.7-9.4 weight % using butt, and the content of the molten albumen of acid is counted as 0.2- using butt
0.8 weight %, cinder is divided into 1.2-1.5 weight % in terms of butt, crude fibre is counted as 2.7-3.4 weight % using butt, with butt
Meter crude fat is 3-3.7 weight %, and moisture is 12-15 weight %；
The DDGS counts crude protein as 23-29 weight % using butt, and the content of the molten albumen of acid is counted as 3.5-5 weights using butt
% is measured, cinder is divided into 4.5-5.5 weight % in terms of butt, counts crude fibre as 10-12 weight % using butt, the crude fat in terms of butt
For 6.6-8 weight %, moisture is 5.5-12 weight %；
The whitewashing maize peel counts crude protein as 17-21 weight % using butt, and the content of the molten albumen of acid is counted as 15- using butt
17 weight %, cinder is divided into 5-6 weight % in terms of butt, counts crude fibre as 7.4-9 weight % using butt, the crude fat in terms of butt
For 1.9-2.3 weight %, moisture is 7.8-12 weight %.
Preferably, the fermentation raw material contain corn starch residue, maize alcohol lees soluble matter, citric acid grain, corn flour,
It is one or more in DDGS and whitewashing maize peel.
It is highly preferred that the fermentation raw material for corn starch residue, maize alcohol lees soluble matter, citric acid grain, corn flour,
DDGS and whitewashing maize peel.
According to the present invention, the considerations of for nutritional ingredient, raw material color and luster and granularity, the fermentation raw material is thick in terms of butt
For protein content on the basis of above-mentioned condition is met, the moisture in preferably described fermentation raw material is 35 weight % hereinafter, institute
It is 10 weight % hereinafter, crude fat is more than 2 weight % in terms of butt to state fermentation raw material crude fibre in terms of butt, thick in terms of butt
Ash content is 10 weight % hereinafter, the content of the molten albumen of acid is more than 15 weight % in terms of butt.
As long as in addition, crude protein content is more than 20 weight % in terms of butt for the fermentation raw material, from raw material color
Pool considers with granularity, the aspect of nutritional ingredient, more preferably 22-24 weight %.
In the present invention, fermentation raw material crude protein in terms of butt refers to use《Crude protein measures in 6432 feeds of GB/T
Method》The data of gained, the molten albumen of acid refers to use in terms of butt《GB/T 22492-2008 soy peptide powder assay methods》Gained
Data, crude fibre refers to use in terms of butt《Crude fibre assay method in 6434 feeds of GB/T》The data of gained are measured, with butt
Meter crude fat refers to use《The measure of crude fat in 6433 feeds of GB/T》The data of gained are measured, coarse ash refers in terms of butt
With《The measure of coarse ash in GB/T6438-2007 feeds》Measure the data of gained.
According to the present invention, to the condition of the fermentation, there is no particular limitation, can be the selection of this field routine.It is preferred that
In the case of, the condition of the fermentation can include：Oxygen content is 0.5-2.5 volume %, preferably 1-2 volumes %；Temperature is
30-40 DEG C, preferably 33-37 DEG C；Time is 96-192 hours, preferably 120-168 hours；Initial ph value is 5-7, preferably
6-7.The present inventor has found by in-depth study, under these conditions, particularly in the range of above-mentioned initial ph value
It ferments, can make the bacillus of activation, the saccharomycete of activation and lactobacillus that can obtain fabulous ferment effect, most
The fermentative feedstuff of microbe raw material with higher strain content is obtained eventually.As the substance for adjusting pH value, there is no particular limitation,
The various reagents for being used to adjust pH value commonly used in the art can be used.Such as can be calcium carbonate and/or sodium hydroxide.
According to the present invention, the fermentation process includes two stages of aerobic fermentation and anaerobic fermentation, in the aerobic fermentation stage,
The bacillus of activation plays main fermentating metabolism effect, when oxygen content is reduced to 0.5 volume %, the saccharomycete of activation
Main fermentating metabolism effect is played, after the oxygen in environment is exhausted, lactobacillus proceeds by anaerobic fermentation, such root
Complete the fermentation of three classes strain simultaneously using one time fermentation process according to the characteristic of strain.Preferably, the fermentation process is impermeable
It is carried out in the packaging bag of gas, the packaging bag is equipped with one-way exhaust valve, and gas only can be by the one-way exhaust valve out of bag
It discharges outside bag.Saccharomycete in order to make lactobacillus, the bacillus of activation and activation can obtain preferable fermentation effect
Fruit, it is preferable that fermentation raw material in bag and the volume ratio of air being sealed in bag are 10：0.5-1, discharge excessive gas sealing
.
In the present invention, unless otherwise specified, " oxygen content " refers to that the volume of oxygen is filled with fermentation in installation for fermenting
Put the ratio of total volume.Oxygen can add in the form of air, oxygen content can by calculate the amount of air that is passed through come
It determines.
The present invention also provides the fermentative feedstuff of microbe raw materials prepared by production method of the present invention.Micro- life
Each component content and the performance of object fermented feed raw material are as previously mentioned, no longer Ao Shu herein.
The present invention also provides a kind of feed, wherein, which contains fermentative feedstuff of microbe raw material of the present invention
And soybean oil.The fermentative feedstuff of microbe raw material can be used for substituting the main components of the materials as feed such as corn, dregs of beans.
Preferably, the fermentative feedstuff of microbe raw material is 4-12 weight % in the content of feed.
As a preferred embodiment of the present invention, the content of mentioned microorganism fermented feed raw material is 8.3-8.4
Weight %, the type and content of other ingredients can be respectively：Corn is 48-49 weight %, and dregs of beans is 35-36 weight %,
Soybean oil is 4-5 weight %, and mountain flour is 1-2 weight %, and calcium monohydrogen phosphate is 1-2 weight %.
It below will the present invention will be described in detail by embodiment and comparative example.In following embodiment and comparative example, always
Acid is passed through by chemical titration, moisture《The measure side of 6435 Moisture in Feed of GB/T and other volatile matter contents
Method》It measures, crude protein passes through in terms of butt《Crude protein determining method in 6432 feeds of GB/T》It measures, the molten egg of acid in terms of butt
Pass through in vain《GB/T 22492-2008 soy peptide powder assay methods》It measures, coarse ash passes through in terms of butt《GB/T 6438-2007
The measure of coarse ash in feed》It measures, crude fibre passes through in terms of butt《Crude fibre assay method in 6434 feeds of GB/T》It surveys
Fixed, crude fat passes through in terms of butt《The measure of crude fat in 6433 feeds of GB/T》It measures.
In following embodiment and comparative examples, corn starch residue is beautiful to produce in the corn deep processings product process such as citric acid
Filter residue of the rice through crushing, liquefying, filter acquisition, then through the dry product obtained；
Maize alcohol lees soluble matter passes through infiltration, crushing, liquefaction, saccharification for corn, then ferments through yeast, second is distilled off
After alcohol, the clear liquid (solid content is 3 weight %) that object filtering obtains is slipped to remaining kettle and is concentrated that (solid content is 35 weights after concentration
Measure %) after product；
Citric acid grain is the filtered remaining filter residue of zymotic fluid during producing citric acid product by fermenting raw materials of corn
Product, moisture 70%；
Corn flour is the powder that corn is grinding to obtain；
DDGS is infiltrated corn, de- plumule, crushing, liquefaction, saccharification, fermentation, the obtained filtrate of process of distillation and clear
Liquid mixes；
Whitewashing maize peel is corn steep liquor to be sprayed onto on maize peel and through the dry product obtained；
In following embodiments, lactobacillus acidophilus (Lactobacillus acidophilus, CGMCC number 1.1854), production
Protein candidiasis (Candida utilis, CGMCC number 2.1180) comes from China General Microbiological culture presevation management
Center, purchased from Agricultural University Of Anhui's Life Science College, number is bacillus subtilis (Bacillus subtilis)
951NA4。
Embodiment 1
(1) bacillus subtilis is activated：At 30 DEG C, bacillus subtilis is put into the glucose of a concentration of 2 weight %
30min is activated in aqueous solution, the bacillus subtilis activated；
(2) candida utili bacterium is activated：At 30 DEG C, candida utili bacterium is activated into 30min into the water, is obtained
The candida utili bacterium of activation；
(3) it ferments：By lactobacillus acidophilus, the candida utili bacterium of the bacillus subtilis of activation and activation and acidophilus breast
Bacillus is mixed to get strain mixed liquor, and 7 parts by weight Corn starch slags, 26 parts by weight Corn alcohol grain soluble matter, 5 parts by weight are beautiful
(the moisture of mixture after rice flour, 10 parts of citric acid grains, 6 parts by weight DDGS and the whitewashing maize peel mixing of 46 parts by weight are broken up
For 33 weight %；The fermentation raw material counts coarse-fibred content as 9.2 weight % using butt, and the content of crude fat is in terms of butt
3.2 weight %, the content for counting coarse ash using butt count the content of the molten albumen of acid as 14.03 weights as 8.89 weight % using butt
Measure %.) (bacillus subtilis of activation, the candida utili bacterium of activation and lactobacillus acidophilus are mixed with strain mixed liquor
Dosage so that the inoculum concentration of bacillus subtilis is 1 × 108Cfu/g, the inoculum concentration of candida utili bacterium is 2 × 106Cfu/g,
The inoculum concentration of lactobacillus acidophilus is 5 × 107Cfu/g), air-locked packaging bag is charged material into, which is equipped with unidirectional
Air bleeding valve, gas can be only discharged out of bag outside bag by the one-way exhaust valve, and the material in packaging bag is with being sealed in bag
The volume ratio of gas is 10：1, it ferments under the following conditions：Oxygen content is 2 volume %, and temperature is 35 DEG C, and the time is
144 hours, starting pH was 7.
After fermentation, smell, pH, acidity and each component content of gained fermentative feedstuff of microbe raw material etc., knot are measured
Fruit is as shown in Table 1 and Table 2.
Embodiment 2
(1) bacillus subtilis is activated：At 20 DEG C, bacillus subtilis is put into the glucose of a concentration of 3 weight %
20min is activated in aqueous solution, the bacillus subtilis activated；
(2) candida utili bacterium is activated：At 20 DEG C, candida utili bacterium is activated into 20min into the water, is obtained
The candida utili bacterium of activation；
(3) it ferments：The candida utili bacterium of lactobacillus acidophilus, the bacillus subtilis of activation and activation is mixed to get
Strain mixed liquor, by 7 parts by weight Corn starch slags, 28 parts by weight Corn alcohol grain soluble matter, 5 parts by weight Corn powder, 8 portions of lemons
(moisture of mixture is 33.5 weight % after sour grain, 6 parts by weight DDGS and the whitewashing maize peel mixing of 46 parts by weight are broken up；
The fermentation raw material counts coarse-fibred content as 8.96 weight % using butt, and the content of crude fat is counted as 3.12 weights using butt
% is measured, the content for counting coarse ash using butt counts the content of the molten albumen of acid as 14.2 weight % as 8.75 weight % using butt.) with
(bacillus subtilis, the candida utili bacterium of activation and the dosage of lactobacillus acidophilus of activation cause the mixing of strain mixed liquor
The inoculum concentration of bacillus subtilis is 8 × 107Cfu/g, the inoculum concentration of candida utili bacterium is 1 × 106Cfu/g, acidophilus breast bar
The inoculum concentration of bacterium is 2.5 × 107Cfu/g), air-locked packaging bag is charged material into, which is equipped with one-way exhaust valve,
Gas can be only discharged out of bag outside bag by the one-way exhaust valve, the material in packaging bag and the body of gas being sealed in bag
Product is than being 10：1, it ferments under the following conditions：Oxygen content is 1.5 volume %, and temperature is 33 DEG C, and the time is 120 hours,
It is 6 to originate pH.
After fermentation, smell, pH, acidity and each component content of gained fermentative feedstuff of microbe raw material etc., knot are measured
Fruit is as shown in Table 1 and Table 2.
Embodiment 3
(1) bacillus subtilis is activated：At 40 DEG C, bacillus subtilis is put into the glucose of a concentration of 1 weight %
40min is activated in aqueous solution, the bacillus subtilis activated；
(2) candida utili bacterium is activated：At 40 DEG C, candida utili bacterium is activated into 40min into the water, is obtained
The candida utili bacterium of activation；
(3) it ferments：The candida utili bacterium of lactobacillus acidophilus, the bacillus subtilis of activation and activation is mixed to get
Strain mixed liquor, by 7 parts by weight Corn starch slags, 30 parts by weight Corn alcohol grain soluble matter, 5 parts by weight Corn powder, 6 portions of lemons
(moisture of mixture is 34 weight % after sour grain, 6 parts by weight DDGS and the whitewashing maize peel mixing of 46 parts by weight are broken up；Institute
It states fermentation raw material and coarse-fibred content is counted as 8.7 weight % using butt, the content of crude fat is counted as 3.1 weight % using butt, with
The content of butt meter coarse ash is 8.73 weight %, and the content of the molten albumen of acid is counted as 14.3 weight % using butt.) mixed with strain
(bacillus subtilis, the candida utili bacterium of activation and the dosage of lactobacillus acidophilus of activation cause withered grass gemma for liquid mixing
The inoculum concentration of bacillus is 5 × 107Cfu/g, the inoculum concentration of candida utili bacterium is 1.5 × 106Cfu/g, lactobacillus acidophilus connect
Kind amount is 1 × 107Cfu/g), air-locked packaging bag is charged material into, which is equipped with one-way exhaust valve, and gas only may be used
It is discharged outside bag out of bag by the one-way exhaust valve, the volume ratio of gas of the material in packaging bag with being sealed in bag is
10：1, it ferments under the following conditions：Oxygen content is 1 volume %, and temperature is 37 DEG C, and the time is 168 hours, and starting pH is
6.5。
After fermentation, smell, pH, acidity and each component content of gained fermentative feedstuff of microbe raw material etc., knot are measured
Fruit is as shown in Table 1 and Table 2.
Embodiment 4
Method according to embodiment 1 prepares fermentative feedstuff of microbe raw material, unlike, the starting pH of fermentation process is 5.
After fermentation, smell, pH, acidity and each component content of gained fermentative feedstuff of microbe raw material etc., knot are measured
Fruit is as shown in Table 1 and Table 2.
Embodiment 5
Method according to embodiment 1 prepares fermentative feedstuff of microbe raw material, unlike, the starting pH of fermentation process is
4.2。
After fermentation, smell, pH, acidity and each component content of gained fermentative feedstuff of microbe raw material etc., knot are measured
Fruit is as shown in Table 1 and Table 2.
Embodiment 6
Method according to embodiment 1 prepares fermentative feedstuff of microbe raw material, unlike, oxygen content is 0.8 volume %.
After fermentation, smell, pH, acidity and each component content of gained fermentative feedstuff of microbe raw material etc., knot are measured
Fruit is as shown in Table 1 and Table 2.
Embodiment 7
Method according to embodiment 1 prepares fermentative feedstuff of microbe raw material, unlike, fermentation time is 48 hours.
After fermentation, smell, pH, acidity and each component content of gained fermentative feedstuff of microbe raw material etc., knot are measured
Fruit is as shown in Table 1 and Table 2.
Comparative example 1
Method according to embodiment 1 prepares fermentative feedstuff of microbe raw material, unlike, bacillus subtilis and production protein are false
Silk saccharomycete is not activated, and is directly added into fermentation raw material and is fermented.
After fermentation, the performance of gained fermentative feedstuff of microbe raw material is as shown in Table 1 and Table 2.
Comparative example 2
Method according to embodiment 1 prepares fermentative feedstuff of microbe raw material, unlike, the activation side of bacillus subtilis
Formula is：At 30 DEG C, bacillus subtilis is activated into 30min into the water, the bacillus subtilis activated.
After fermentation, the performance of fermentative feedstuff of microbe raw material obtained is as shown in Table 1 and Table 2.
Comparative example 3
Method according to embodiment 1 prepares fermentative feedstuff of microbe raw material, unlike, the activation of candida utili bacterium
Mode is：At 40 DEG C, candida utili bacterium is put into the glucose solution of a concentration of 1 weight % and activates 40min, is obtained
To the candida utili bacterium of activation.
After fermentation, the performance of fermentative feedstuff of microbe raw material obtained is as shown in Table 1 and Table 2.
Table 1
Note：In table 1 bacillus, saccharomycete and lactobacillus refer respectively to bacillus subtilis, candida utili bacterium and
Lactobacillus acidophilus；Moisture and total acid content are on the basis of the total weight of microbial fermentation raw material in table 1.
Table 2
Note：The molten albumen increase rate of acid=(sour in weight-fermentation raw material of the molten albumen of acid in fermentative feedstuff of microbe raw material
The weight of molten albumen) weight × 100% of the molten albumen of acid in/fermentation raw material；Crude fibre, crude protein, the molten albumen of acid, crude fat and
The content of coarse ash be all in terms of the butt in fermentative feedstuff of microbe raw material, surplus for starch, minerals and inevitably
Impurity.
It can be seen from the result of above example then the present invention to strain Selective activation by adding proferment
The fermentative feedstuff of microbe raw material that the method for material is prepared has abundant nutriment, and with strong acid perfume and alcohol fragrance
Taste helps to improve the appetite and production performance of animal, also, the method for the invention is avoided and answered using preparation process
The liquid seed liquor miscellaneous, retention cycle is short is only fermented to being directly added into fermentation raw material after strain progress Selective activation,
Preparation method is simple.
By the results contrast of embodiment 1 and comparative example 1 it is found that the present invention carries out three kinds of bacterium the method ratio of Selective activation
The performance of fermentative feedstuff of microbe raw material prepared by the method that the three kinds of bacterium not activated completely are directly added into fermentation raw material is more excellent；
By the results contrast of embodiment 1 and comparative example 2,3 it is found that present invention glucose solution activates bacillus subtilis, uses water
The method of activation candida utili bacterium is more advantageous to preparing the fermentative feedstuff of microbe raw material haveing excellent performance.
The preferred embodiment of the present invention has been described above in detail, still, during present invention is not limited to the embodiments described above
Detail, within the scope of the technical concept of the present invention, a variety of simple variants can be carried out to technical scheme of the present invention, this
A little simple variants all belong to the scope of protection of the present invention.
It is further to note that specific technical features described in the above specific embodiments, in not lance
In the case of shield, can be combined by any suitable means, in order to avoid unnecessary repetition, the present invention to it is various can
The combination of energy no longer separately illustrates.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally
The thought of invention, it should also be regarded as the disclosure of the present invention.
Claims (10)
1. a kind of fermentative feedstuff of microbe raw material, which is characterized in that in the fermentative feedstuff of microbe raw material, bacillus contains
Amount 2 × 108-6×108Cfu/g, the content 5 × 10 of saccharomycete6-7×106Cfu/g, the content 2 × 10 of lactobacillus8-6×
108Cfu/g counts total acid content as 4-6 weight % using lactic acid, the coarse-fibred content 8-9 weight % in terms of butt, thick in terms of butt
The content 22-23 weight % of albumen, the content 3.2-3.4 weight % of crude fat, the content of coarse ash in terms of butt in terms of butt
8.8-9.2 weight %, the content 15.5-16.5 weight % of the molten albumen of acid in terms of butt.
2. fermentative feedstuff of microbe raw material according to claim 1, wherein, in the fermentative feedstuff of microbe raw material, bud
The content 4 × 10 of spore bacillus8-5.5×108Cfu/g, the content 6 × 10 of saccharomycete6-7×106Cfu/g, the content of lactobacillus
2.5×108-6×108Cfu/g counts total acid content as 5-6 weight %, the coarse-fibred content 8.3-8.65 in terms of butt using lactic acid
Weight %, the content 22.6-23 weight % of crude protein in terms of butt, the content 3.21-3.4 weight % of crude fat in terms of butt,
The content 8.85-9.2 weight % of coarse ash in terms of butt, the content 16.2-16.5 weight % of the molten albumen of acid in terms of butt；
Preferably, the lactobacillus is one or more, the bud in lactobacillus acidophilus, lactobacillus plantarum and lactobacillus fermenti
Spore bacillus is one or more in bacillus subtilis, bacillus licheniformis and bacillus coagulans, and the saccharomycete is production
Protein candidiasis and/or S. cervisiae；
It is further preferred that the lactobacillus be lactobacillus acidophilus, the bacillus be bacillus subtilis, the saccharomycete
For candida utili bacterium.
3. a kind of production method of fermentative feedstuff of microbe raw material, which is characterized in that the production method includes：Activate bacillus
And the step of activated yeast and lactobacillus, the bacillus of activation and the saccharomycete of activation are added in fermentation raw material
The step of being fermented；
Activation bacillus mode be：Under conditions of 20-40 DEG C, bacillus is placed in the Portugal of a concentration of 1-3 weight %
20-40min in grape sugar aqueous solution；
The mode of activated yeast is：Under conditions of 20-40 DEG C, saccharomycete is placed in water 20-40min；
The fermentation raw material contains corn starch residue, maize alcohol lees soluble matter, citric acid grain, corn flour, distiller's dried grain and its can
Molten object and whitewashing maize peel in it is one or more, and the fermentation raw material using butt count crude protein content as 20 weight % with
On.
4. production method according to claim 3, wherein, relative to every gram of fermentation raw material, the bacillus connects
Kind amount is 1 × 107-1×108Cfu, the inoculum concentration of the lactobacillus is 1 × 106-5×107Cfu, the inoculum concentration of the saccharomycete
It is 1 × 105-2×106cfu。
Preferably, relative to every gram of fermentation raw material, the inoculum concentration of the bacillus is 5 × 107-1×108Cfu, the breast
The inoculum concentration of bacillus is 1 × 107-5×107Cfu, the inoculum concentration of the saccharomycete is 1 × 106-2×106cfu。
5. production method according to claim 3 or 4, wherein, bacillus is bacillus subtilis, bacillus licheniformis
With it is one or more in bacillus coagulans, saccharomycete be candida utili bacterium and/or S. cervisiae, lactobacillus for plant
It is one or more in object lactobacillus, lactobacillus acidophilus and lactobacillus fermenti；
Preferably, the bacillus is bacillus subtilis, and the saccharomycete is candida utili bacterium, and the lactobacillus is
Lactobacillus acidophilus.
6. production method according to claim 3 or 4, wherein, the condition of the fermentation includes：Oxygen content is 0.5-
2.5 volume %, temperature are 30-40 DEG C, and the time is 96-192 hours, initial ph value 5-7；
Preferably, the condition of the fermentation includes：Oxygen content is 1-2 volume %, and temperature is 33-37 DEG C, time 120-168
Hour, initial ph value 6-7.
7. production method according to claim 3 or 4, wherein, the fermentation process carries out in air-locked packaging bag,
The packaging bag is equipped with one-way exhaust valve, and gas can be only discharged out of bag outside bag by the one-way exhaust valve.
8. production method according to claim 3 or 4, wherein, the moisture in the fermentation raw material is 35 weight %
Below；The fermentation raw material coarse-fibred content in terms of butt is 10 weight % hereinafter, the content of crude fat is 2 weights in terms of butt
More than % is measured, the content of coarse ash is 10 weight % hereinafter, the content of the molten albumen of acid is 15 weight % in terms of butt in terms of butt
More than.
9. the fermentative feedstuff of microbe raw material prepared as the production method described in any one in claim 3-8.
10. a kind of feed, which is characterized in that the feed contains the microbial fermentation described in any one in claim 1,2 and 9
Feedstuff and soybean oil.
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