CN103074284B - Zymophyte liquid, liquid spirit stillage fermented feed and fermentation method of liquid spirit stillage fermented feed - Google Patents
Zymophyte liquid, liquid spirit stillage fermented feed and fermentation method of liquid spirit stillage fermented feed Download PDFInfo
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- CN103074284B CN103074284B CN201310031536.5A CN201310031536A CN103074284B CN 103074284 B CN103074284 B CN 103074284B CN 201310031536 A CN201310031536 A CN 201310031536A CN 103074284 B CN103074284 B CN 103074284B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a zymophyte liquid, a liquid spirit stillage fermented feed and a fermentation method of the liquid spirit stillage fermented feed. The zymophyte liquid is prepared by mixing, at a preset proportion, liquids of lactobacillus, bacillus subtilis, bacillus natto and candida utilis bacteria which are obtained by enlarged culture. The fermented feed is prepared by fermenting a liquid complete feed with the zymophyte liquid. The fermentation method comprises the steps of inoculating the zymophyte liquid with the liquid complete feed at 3-5%wt, adjusting the moisture content to be 68-72%, sufficiently mixing into a liquid, and loading into a sealer for sealing and fermentation. The shelf life of the liquid spirit stillage fermented feed fermented by the zymophyte liquid is over 6 months at a room temperature. The liquid spirit stillage fermented feed is easier to digest, low in thallus activity loss and ideal in using effect, a culture proportion and a complete feed formula balance essential amino acid, and the liquid spirit stillage fermented feed is suitable for a whole postweaning growth stage of a commercial pig.
Description
Technical field
The invention belongs to biotechnology and fodder production field, relate to specifically a kind of zymocyte liquid, and the feed that utilizes this zymocyte liquid to carry out biological fermentation acquisition, meanwhile, also relate to the method for this biological fermentation.
Background technology
Antibiotic abuse has hindered livestock-raising to development green, healthy, less energy-consumption direction, and producing, apply green safety feed has become future in feed industry developing direction.
Fermented feed is eliminated antinutritional factor with it by fermentation, improve the digestibility of food consumption and nutritive substance, improve the active health level of animal, reduce digestive tract diseases incidence, reduce the discharge of ammonia and foul smell, improve the ecological environment, meet the requirement of energy-saving and emission-reduction, fermented feed technical development is in recent years very fast.
In addition, what the brewed spirit industry of China produced every year loses poor output more than 3,500 ten thousand tons, these fresh distillers ' grains acidity are large, moisture content is more than 60%, and the crude fat that contains 10% left and right, the crude protein of 17% left and right are, inorganic elementss such as robust fibre, vitamin B group and the phosphorus of 25% left and right, potassium.In addition, also have the micro-effective constituents such as amino acid, Yeast Nucleic Acid and purine that produced by microbial cells in distillers ' grains, these are all that cereal can not be compared.
Therefore, to distillers ' grains, the applied research in livestock-raising is always continuous both at home and abroad, and various application reports, achievement in research emerge in an endless stream.In the middle of this, take that to utilize distillers ' grains and/or coordinate more than one other cereal materials be substratum, by one or more microorganisms or zymin, continue fermentation for maximum, this method has brought into play the advantage of fermented feed, and a large amount of distillers ' grains resources has also obtained utilization simultaneously.But the product that this method ferments out also exists, and quality is low, living contaminants is serious, perishable, ferment unstable, result of use is undesirable and production cost, the relatively high defect of equipment requirements, large-scale promotion difficulty is large.
Summary of the invention
Given this, the object of the present invention is to provide a kind of littlely without living contaminants or pollution level, fermented quality is high, the strain combination thing of fermentation stability; And provide a kind of not perishable feed; A kind of feed fermentation method that can large-scale promotion is also provided.
For solving above technical problem, technical scheme provided by the invention is, a kind of fodder zymophyte liquid is provided, and is that the bacterium liquid of the lactobacillus obtaining through enlarged culturing, subtilis, bacillus natto and Candida utilis bacterium is mixed by preset ratio.
Preferably, lactobacillus, subtilis, bacillus natto and Candida utilis bacterium through the cultivation of one-level solid body, secondary liquid culture, finally obtain bacterium liquid separately respectively.
Further, cultivate the lactobacillus liquid concentration > 2 * 10 obtaining
9cFU/mL, subtilis liquid concentration > 1 * 10
10cFU/mL, bacillus natto liquid concentration > 5 * 10
9cFU/mL, Candida utilis bacterial concentration > 1 * 10
10cFU/mL.
Preferably, described lactobacillus liquid, subtilis liquid, bacillus natto liquid and Candida utilis bacterium liquid by volume 4:1～2:2～1:3 mix.
Preferably, above-mentioned lactobacillus liquid concentration 2.5 * 10
9cFU/mL, subtilis liquid concentration 1.2 * 10
10cFU/mL, bacillus natto liquid concentration 5.8 * 10
9cFU/mL, Candida utilis bacterial concentration 1.2 * 10
10cFU/mL, above bacterium liquid by volume 4:1.5:1.5:3 mixes.
The present invention also provides a kind of liquid distillers ' grains fermented feed, liquid complete feed makes through above-mentioned zymocyte liquid fermentation, described each component of liquid complete feed is respectively by weight: 280～350 parts, the fresh white vinasse that moisture is 60～65%, 280～330 parts of corns, 50～70 parts of wheats, 50～100 parts, wheat bran, 25～40 parts of dregs of beans, 35～45 parts of cotton dregs, 25～35 parts of the dish dregs of rice, 25～35 parts of blood meals, 45～60 parts, molasses, 15～25 parts of soybean oil, 10～13 parts of secondary calcium phosphates, 8～10 parts of calcium powders, 3.6～3.8 parts of salt, 3.6～3.9 parts of Methionins, 0.9～1.2 part of methionine(Met), 0.9～1.2 part of Threonine, 1.8～2.2 parts of tryptophanes, pig is added 5～5.2 parts with ore deposit, 1～1.1 part of multidimensional for pig.This liquid white spirit grain fermented feed is added in pig feed and fed according to 25%～100%.
Preferably, the moisture content of described fresh white vinasse is 60～65%.
Preferably, described each component of liquid complete feed is respectively by weight: moisture is 282 parts, 62% fresh white vinasse, 320 parts of corns, 55 parts of wheats, 100 parts, wheat bran, 40 parts of dregs of beans, 40 parts of cotton dregs, 25 parts of the dish dregs of rice, 30 parts of blood meals, 55 parts, molasses, 15 parts of soybean oil, 11 parts of secondary calcium phosphates, 9 parts of calcium powders, 3.8 parts of salt, 3.8 parts of Methionins, 1 part of methionine(Met), 1.2 parts of Threonines, 2.2 parts of tryptophanes, pig is added 5 parts with ore deposit, 1 part of multidimensional for pig.
The present invention also provides the fermentation process of above-mentioned liquid distillers ' grains fermented feed, described zymocyte liquid and aforesaid liquid complete feed are inoculated by 3～5%wt, and to adjust moisture be 68～72%, fully be mixed into liquid, pack sealer sealing into, at leavening temperature, be 25～30 ℃ of condition bottom fermentations 3 days or be 18～24 ℃ of condition bottom fermentations 5 days at leavening temperature.The feed obtaining by this fermentation process fermentation, the quality guaranteed period is more than 6 months, the longlyest reaches 1 year.
Preferably, described zymocyte liquid and aforesaid liquid complete feed are pressed to 5%wt inoculation, and to adjust moisture being 70%, being fully mixed into liquidly, pack sealer sealing into, is 26 ℃ of condition bottom fermentations 3 days at leavening temperature.
Compared with prior art, the present invention has selected milk-acid bacteria, subtilis, bacillus natto and Candida utilis bacterium, and carries out rational inoculating proportion, by synergistic bacterium effect, suppress varied bacteria growing, fermenting process reaches balance fast, and can well maintain equilibrium state.
The quality guaranteed period that the liquid distillers ' grains fermented feed obtaining through zymocyte liquid fermentation of the present invention is preserved is at normal temperatures more than 6 months.Liquid distillers ' grains fermented feed of the present invention is easier to digestion, and microbial activity loss is little, and result of use is desirable, this inoculating proportion and complete diet pellet balance formula necessary amino acid, be applicable to the whole growth phases after market pig wean.
Without sterilizing, after having fermented, need not dry before solution culture fermentation, make that liquid white spirit grain fermented feed production cost is low, production technology and equipments requirement is simple, meet the demand that the technical scaleization of succinct practicality is produced.
Liquid feed of the present invention, the problems such as unstable, the perishable and cost of quality that existing fermented feed exists, equipment requirements height have been solved, being applicable to large-scale industrialization raw, giving the added value that product is higher simultaneously, is the Perfected process of the fresh distillers ' grains of scientific utilization.
Embodiment
Embodiment 1:
Slant strains lactobacillus
lacticacid bacteria, subtilis
b.subtilis, bacillus natto
bacillus nattowith Candida utilis bacterium
candida utilisafter one-level solid culture, secondary liquid culture, lactobacillus liquid concentration 2.5 * 10
9cFU/mL, subtilis liquid concentration 1.2 * 10
10cFU/mL, bacillus natto liquid concentration 5.8 * 10
9cFU/mL, Candida utilis bacterial concentration 1.2 * 10
10cFU/mL, 4:1.5:1.5:3 mixes by volume, bacterium liquid cell concn 7.3 * 10
9cFU/mL, makes zymocyte liquid.
Selecting moisture is 282 parts, 62% fresh white vinasse, 320 parts of corns, 55 parts of wheats, 100 parts, wheat bran, 40 parts of dregs of beans, 40 parts of cotton dregs, 25 parts of the dish dregs of rice, 30 parts of blood meals, 55 parts, molasses, 15 parts of soybean oil, 11 parts of secondary calcium phosphates, 9 parts of calcium powders, 3.8 parts of salt, 3.8 parts of Methionins, 1 part of methionine(Met), 1.2 parts of Threonines, 2.2 parts of tryptophanes, pig is added 5 parts with ore deposit, and 1 part of multidimensional for pig, is made into liquid complete feed.
Zymocyte liquid and liquid complete feed are pressed 5%wt inoculation, and adjusting moisture is 70%, are fully mixed into liquidly, pack bucket or pocket sealing into, and 26 ℃ of fermentations 3 days, obtain liquid white spirit grain fermented feed.
The key technical indexes of this product is as follows:
Content of microorganisms 1.2 * 10
9cFU/g, pH < 3.8, protein value content > 16.2%, pig digestible energy 3.05Mcal/kg, the normal temperature time of guaranteeing the quality is 12 months.
Embodiment 2:
Slant strains lactobacillus
lacticacid bacteria, subtilis
b.subtilis, bacillus natto
bacillus nattowith Candida utilis bacterium
candida utilisafter level liquid cultivation, secondary liquid culture, lactobacillus liquid concentration 2.2 * 10
9cFU/mL, subtilis liquid concentration 1.2 * 10
10cFU/mL, bacillus natto liquid concentration 5.6 * 10
9cFU/mL, Candida utilis bacterial concentration 1.1 * 10
10cFU/mL, 4:1.4:1.6:3 mixes by volume, bacterium liquid cell concn 6.7 * 10
9cFU/mL, makes zymocyte liquid.
Selecting moisture is 322 parts, 62% fresh white vinasse, 300 parts of corns, 65 parts of wheats, 60 parts, wheat bran, 35 parts of dregs of beans, 45 parts of cotton dregs, 30 parts of the dish dregs of rice, 30 parts of blood meals, 50 parts, molasses, 25 parts of soybean oil, 13 parts of secondary calcium phosphates, 8 parts of calcium powders, 3.8 parts of salt, 3.6 parts of Methionins, 0.9 part of methionine(Met), 0.9 part of Threonine, 1.8 parts of tryptophanes, pig is added 5 parts with ore deposit, and 1 part of multidimensional for pig, is made into liquid complete feed.
Zymocyte liquid and liquid complete feed are pressed 5%wt inoculation, and adjusting moisture is 72%, are fully mixed into liquidly, pack bucket or pocket sealing into, and 26 ℃ of fermentations 3 days, obtain liquid white spirit grain fermented feed.
The key technical indexes of this product is as follows:
Content of microorganisms 1 * 10
9cFU/g, pH < 3.8, protein value content > 16.5%, pig digestible energy 3.1Mcal/kg, normal temperature is guaranteed the quality 12 months time.
Below be only the preferred embodiment of the present invention, it should be pointed out that above-mentioned preferred implementation should not be considered as limitation of the present invention, protection scope of the present invention should be as the criterion with claim limited range.For those skilled in the art, without departing from the spirit and scope of the present invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (6)
1. a fodder zymophyte liquid, is characterized in that, is the bacterium liquid of the lactobacillus obtaining through enlarged culturing, subtilis, bacillus natto and Candida utilis bacterium is mixed by preset ratio; Lactobacillus, subtilis, bacillus natto and Candida utilis bacterium, respectively through one-level solid culture, secondary liquid culture, finally obtain bacterium liquid separately; The lactobacillus liquid concentration > 2 * 10 that cultivation obtains
9cFU/mL, subtilis liquid concentration > 1 * 10
10cFU/mL, bacillus natto liquid concentration > 5 * 10
9cFU/mL, Candida utilis bacterial concentration > 1 * 10
10cFU/mL; Described lactobacillus liquid, subtilis liquid, bacillus natto liquid and Candida utilis bacterium liquid is 4:(1～2 by volume): (2～1): 3 mix.
2. fodder zymophyte liquid according to claim 4, is characterized in that, above-mentioned lactobacillus liquid concentration 2.5 * 10
9cFU/mL, subtilis liquid concentration 1.2 * 10
10cFU/mL, bacillus natto liquid concentration 5.8 * 10
9cFU/mL, Candida utilis bacterial concentration 1.2 * 10
10cFU/mL, above bacterium liquid by volume 4:1.5:1.5:3 mixes.
3. a liquid distillers ' grains fermented feed, it is characterized in that, liquid complete feed makes through above-mentioned zymocyte liquid fermentation, described each component of liquid complete feed is respectively by weight: 280～350 parts, the fresh white vinasse that moisture is 60～65%, 280～330 parts of corns, 50～70 parts of wheats, 50～100 parts, wheat bran, 25～40 parts of dregs of beans, 35～45 parts of cotton dregs, 25～35 parts of the dish dregs of rice, 25～35 parts of blood meals, 45～60 parts, molasses, 15～25 parts of soybean oil, 10～13 parts of secondary calcium phosphates, 8～10 parts of calcium powders, 3.6～3.8 parts of salt, 3.6～3.9 parts of Methionins, 0.9～1.2 part of methionine(Met), 0.9～1.2 part of Threonine, 1.8～2.2 parts of tryptophanes, pig is added 5～5.2 parts with ore deposit, 1～1.1 part of multidimensional for pig.
4. liquid distillers ' grains fermented feed according to claim 3, is characterized in that, described each component of liquid complete feed is respectively by weight: moisture is 282 parts, 62% fresh white vinasse, 320 parts of corns, 55 parts of wheats, 100 parts, wheat bran, 40 parts of dregs of beans, 40 parts of cotton dregs, 25 parts of the dish dregs of rice, 30 parts of blood meals, 55 parts, molasses, 15 parts of soybean oil, 11 parts of secondary calcium phosphates, 9 parts of calcium powders, 3.8 parts of salt, 3.8 parts of Methionins, 1 part of methionine(Met), 1.2 parts of Threonines, 2.2 parts of tryptophanes, pig is added 5 parts with ore deposit, 1 part of multidimensional for pig.
5. the fermentation process of liquid distillers ' grains fermented feed described in claim 3 or 4, it is characterized in that, described zymocyte liquid and aforesaid liquid complete feed are inoculated by 3～5%wt, and to adjust moisture be 68～72%, fully be mixed into liquid, pack sealer sealing into, at leavening temperature, be 25～30 ℃ of condition bottom fermentations 3 days or be 18～24 ℃ of condition bottom fermentations 5 days at leavening temperature.
6. the fermentation process of liquid distillers ' grains fermented feed according to claim 4, it is characterized in that, described zymocyte liquid and aforesaid liquid complete feed are pressed to 5%wt inoculation, and to adjust moisture be 70%, fully be mixed into liquid, packing sealer sealing into, is 26 ℃ of condition bottom fermentations 3 days at leavening temperature.
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| CN103783318A (en) * | 2014-01-20 | 2014-05-14 | 肇庆市端州区广洪机电设备有限公司 | Fermented feed and processing process thereof |
| CN103931951B (en) * | 2014-02-18 | 2015-07-15 | 山东省农业科学院畜牧兽医研究所 | Method for preparing whole wheat capable of being fed for cows and pigs |
| CN103947828A (en) * | 2014-05-06 | 2014-07-30 | 安徽东方新新生物技术有限公司 | Feed prepared by biologically fermenting white spirit vinasse and blood powder |
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| CN105211628A (en) * | 2014-06-10 | 2016-01-06 | 河南省百卓生态农业有限公司 | A kind of production method of liquid nutritional agent |
| CN105146060A (en) * | 2015-10-19 | 2015-12-16 | 高大元 | Method for preparing animal feed by fermentation of rumen microorganisms |
| CN105713861A (en) * | 2016-03-16 | 2016-06-29 | 四川大学 | Method for preparing novel bacteria culture medium from Maotai-flavor liquor vinasse |
| CN106359876A (en) * | 2016-08-26 | 2017-02-01 | 黑龙江蓬勃牧草有限公司 | Preparation technology of complete mixed fermentation feed |
| CN107232420A (en) * | 2017-07-12 | 2017-10-10 | 郭庆 | A kind of distillers ' grains pig feed and its production method |
| CN107594082A (en) * | 2017-09-11 | 2018-01-19 | 辽宁爱普罗斯饲料有限公司 | A kind of liquid raw material fermentation wine brewing lees feedses preparation method and feeding method |
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