CN107603939A - A kind of salt tolerant bacterium culture medium nutritional agents and preparation method thereof and acclimation method and application - Google Patents
A kind of salt tolerant bacterium culture medium nutritional agents and preparation method thereof and acclimation method and application Download PDFInfo
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Abstract
The invention discloses a kind of salt tolerant bacterium culture medium nutritional agents and preparation method thereof and acclimation method and application.Its salt tolerant bacterium culture medium nutritional agents, including following parts by weight：Water, sodium alginate, Kelp Powder, fish meal, dregs of beans, expanded bean powder, yeast, sargassum, spirulina, vitamin C, Choline Chloride, nicotinic acid, calcium pantothenate, vitamine D3 part, vitamin E, inositol, vitamin A, biotin 2, vitamin B1, vitamin B2, vitamin B12, Vitamin K3, SPP1 powder, calcium monohydrogen phosphate, flour, dry ooze and mineral trace element metal salt compound.Outstanding more Facultative Halophiles can be tamed using the salt tolerant bacterium culture medium nutritional agents, so as to meet the process demand of high-salt wastewater complex environment, reach more preferable clean-up effect and stability.
Description
Technical field
The present invention relates to high-salt wastewater bioanalysis technical field, more particularly, to a kind of salt tolerant bacterium culture medium nutritional agents and
Its preparation method and acclimation method and application.
Background technology
High-salt wastewater, refer to the waste water containing finite concentration organic matter of mass fraction >=1% of salt, wherein containing a large amount of
Inorganic salts, such as Cl-, S042-, Na, Ca2+ plasma.For example seawater is in industrial production, organic chemical industry, printing and dyeing, oil exploitation
Etc. application will produce a large amount of waste water, except containing organic contamination beyond the region of objective existence in these waste water, also containing substantial amounts of inorganic
Salt, it is exactly typical high-salt wastewater, this kind of waste water salt concentration is higher, seriously polluted, it is necessary to could be discharged after processing.Mesh
Before, to handle the method for high-salt wastewater mainly have Physical, physical-chemical process and a bioanalysis, wherein Physical and physical-chemical process
Processing cost is of a relatively high, and bioanalysis has turned into the prefered method of most of high-salt wastewater handling process.
But the inorganic salts in high-salt wastewater have stronger inhibitory action, different bioreactor for treatment to general microorganism
The effect of high-salt wastewater is different, and most of microbe can only be resistant to waste water of the saliferous below 3% in common bioanalysis, work as saliferous
When amount is more than 5%, its treatment effect can substantially reduce, and mainly these high-salt wastewaters can make microbial cell dehydration, matter wall
Separate, suppress growth of microorganism the modes such as dehydrogenase activity reduction, therefore needed using common micro-organisms processing high-salt wastewater
It is diluted, this can cause the waste of great lot of water resources.
Therefore, Facultative Halophiles are cultivated to handle high-salt wastewater be a good direction, such as China's application for a patent for invention number
For the preparation method and its microbial inoculum of a kind of halophilic decontamination bacterial agent disclosed in 200910229165.5, the patented technology is exactly to use
Culture presevation numbering CGMCC No.3314 single salt tolerant strain mode carries out high-salt wastewater processing, due to biological treatment system
System is a sufficiently complex system, adds single bacterial strain and is unable to reach optimization process state.
In order to solve the above problems, China's application for a patent for invention number discloses a kind of salt tolerant denitrogenation for 201310510802.2
Composite bacteria agent and its preparation and application, salt denitrification compound bacteria agent in Denitrification Characteristics and denitrification process by with having nitrite nitrogen
The Halomonas of accumulation, with Denitrification Characteristics and denitrification process without nitrite nitrogen accumulation Halomonas, with Nitrification Characteristics
Bacillus and with salt-tolerant trait Halomonas according to volume ratio 1:1:(10~20):(60~85) mixing composition, is adopted
Denitrogenation processing is carried out to high-salt wastewater with above-mentioned salt tolerant denitrification compound bacteria agent, denitrification percent is at least 95%, effluent quality row up to standard
Put.Four kinds of microbial interactions in composite bacteria agent, influence each other, ammonia nitrogen and nitrogen removal rate are single higher than any microorganism
The effect solely used, because the tolerance impact capacity of composite bacteria agent is stronger than the bacterial strain of purifying, simultaneous nitrification and denitrification nitre can be effectively facilitated
The progress of change, significantly improves high-salt wastewater denitrification effect.
There is no universality to tame Facultative Halophiles using existing salt tolerant bacterium culture medium nutritional agents, may be only available for a small number of bacterium
Kind, outstanding more Facultative Halophiles can not be tamed, so as to meet the process demand of high-salt wastewater complex environment, reach more preferable clean-up effect
And stability.
The content of the invention
The primary and foremost purpose of the present invention is to provide a kind of culture medium nutritional agents that can cultivate a variety of Facultative Halophiles, can tame out
Numerous Facultative Halophiles, so as to meet the process demand of high-salt wastewater complex environment, reach more preferable clean-up effect and stability.
The further object of the present invention is to provide a kind of preparation method such as above-mentioned culture medium nutritional agents.
Third object of the present invention is to provide the method for the domestication Facultative Halophiles according to above-mentioned salt tolerant bacterium culture medium nutritional agents.
Fourth object of the present invention is to provide application of the above-mentioned salt tolerant bacterium culture medium nutritional agents in terms of Facultative Halophiles domestication
With the application in terms of high-salt wastewater processing.
In order to solve the above technical problems, the technical solution adopted by the present invention is：
A kind of salt tolerant bacterium culture medium nutritional agents, including following parts by weight：Water 30000-50000 parts, sodium alginate 8000-
12000 parts, Kelp Powder 18000-22000 parts, fish meal 18000-22000 parts, dregs of beans 8000-12000 parts, expanded bean powder 8000-
12000 parts, yeast 4000-6000 parts, sargassum 8000-12000 parts, spirulina 4000-6000 parts, vitamin C 30-44 parts,
Choline Chloride 20-30 parts, nicotinic acid 4.5-7.5 parts, calcium pantothenate 4-6 parts, vitamine D3 2.5-3.5 parts, vitamin E 4-6 parts, flesh
Alcohol 4-5 parts, vitamin A 5-7 parts, the 0.5-1.5 parts of biotin 2, vitamin B1 1.5-2.5 parts, vitamin B2 1.5-2.5
Part, vitamin B12 0.4-0.6 parts, Vitamin K3 0.6-1.4 parts, SPP1 powder 600-1000 parts, calcium monohydrogen phosphate 880-1320
Part, flour 16000-20000 parts, dry ooze 6000-8000 parts and 520-560 part mineral trace element metal salt compounds.
Preferably, the mineral trace element metal salt compound includes：433.3 parts of magnesium sulfate monohydrate, sulfuric acid monohydrate
Ferrous 76.7 parts, 7.1 parts of copper sulfate monohydrate, 17.1 parts of monohydrate zinc sulphate, 10.1 parts of manganese sulfate monohydrate, 0.3 part of sulfuric acid monohydrate cobalt
With 0.26 part of KI.
Preferably, including following parts by weight：50000 parts of water, 10000 parts of sodium alginate, 20000 parts of Kelp Powder, fish meal
20000 parts, 10000 parts of dregs of beans, 10000 parts of expanded bean powder, 5000 parts of yeast, 10000 parts of sargassum, 5000 parts of spirulina, dimension
Raw plain 36 parts of C, 26 parts of Choline Chloride, 6 parts of nicotinic acid, 5 parts of calcium pantothenate, 3 parts of vitamine D3,5 parts of vitamin E, 4.5 parts of inositol,
6 parts of vitamin A, 21 parts of biotin, 2 parts of vitamin B1,2 parts of vitamin B2,0.5 part of vitamin B12, Vitamin K3 1
Part, 800 parts of SPP1 powder, 433.3 parts of magnesium sulfate monohydrate, 76.7 parts of ferrous sulfate monohydrate, 7.1 parts of copper sulfate monohydrate, a water sulphur
Sour 17.1 parts of zinc, 10.1 parts of manganese sulfate monohydrate, 0.3 part of sulfuric acid monohydrate cobalt, 0.26 part of KI, 1000 parts of calcium monohydrogen phosphate, flour
18000 parts and dry 8000 parts of ooze.
Preferably, including following parts by weight：40000 parts of water, 8000 parts of sodium alginate, 22000 parts of Kelp Powder, fish meal
18000 parts, 8000 parts of dregs of beans, 12000 parts of expanded bean powder, 4000 parts of yeast, 12000 parts of sargassum, 6000 parts of spirulina, dimension life
Plain 44 parts of C, 30 parts of Choline Chloride, 7.5 parts of nicotinic acid, 6 parts of calcium pantothenate, 3.5 parts of vitamine D3,6 parts of vitamin E, 5 parts of inositol,
7 parts of vitamin A, 2 1.5 parts of biotin, 2.5 parts of vitamin B1,2.5 parts of vitamin B2,0.6 part of vitamin B12, dimension life
Plain 1.4 parts of K3,1000 parts of SPP1 powder, 433.3 parts of magnesium sulfate monohydrate, 76.7 parts of ferrous sulfate monohydrate, copper sulfate monohydrate 7.1
Part, 17.1 parts of monohydrate zinc sulphate, 10.1 parts of manganese sulfate monohydrate, 0.3 part of sulfuric acid monohydrate cobalt, 0.26 part of KI, calcium monohydrogen phosphate 880
7000 parts of part, 20000 parts of flour and dry ooze.
Preferably, including following parts by weight：30000 parts of water, 12000 parts of sodium alginate, 18000 parts of Kelp Powder, fish meal
22000 parts, 12000 parts of dregs of beans, 8000 parts of expanded bean powder, 6000 parts of yeast, 8000 parts of sargassum, 4000 parts of spirulina, dimension life
Plain 30 parts of C, 20 parts of Choline Chloride, 45 parts of nicotinic acid, 4 parts of calcium pantothenate, 25 parts of vitamine D3,4 parts of vitamin E, 4 parts of inositol, dimension
Raw plain 5 parts of A, 2 0.5 parts of biotin, 1.5 parts of vitamin B1,1.5 parts of vitamin B2,0.4 part of vitamin B12, vitamin
0.6 part of K3,600 parts of SPP1 powder, 433.3 parts of magnesium sulfate monohydrate, 76.7 parts of ferrous sulfate monohydrate, 7.1 parts of copper sulfate monohydrate,
17.1 parts of monohydrate zinc sulphate, 10.1 parts of manganese sulfate monohydrate, 0.3 part of sulfuric acid monohydrate cobalt, 0.26 part of KI, calcium monohydrogen phosphate 1320
6000 parts of part, 16000 parts of flour and dry ooze.
The present invention also provides a kind of preparation method of above-mentioned salt tolerant bacterium culture medium nutritional agents, comprises the following steps：
(1) it is, first that sodium alginate is stand-by with water-solubleization；
(2), Kelp Powder, fish meal, dregs of beans, expanded bean powder, yeast, sargassum, spirulina, dry ooze are mixed crushing and treated
With；
(3), vitamin C and Choline Chloride SPP1 powder are diluted stand-by；
(4), by nicotinic acid, calcium pantothenate, dimension ox element D3, vitamin E, inositol, Wei Shengsuo A, Wei Shengsuo H2, vitamin B1, dimension
Raw plain B2, vitamin B6, vitamin B12 and Vitamin K3 SPP1 powder dilute stand-by；
(5), mineral trace element metal salt compound is diluted with calcium monohydrogen phosphate stand-by；
(6), flour and step (2)~(5) stand-by raw material are mixed together uniformly, being further crushed to pass through
80-200 mesh sieves；
(7), stand-by solution prepared by step (1) is added in the powder obtained to step (6) and stirred and evenly mixed；
(8) drained after, being soaked 30 seconds with 5% calcium chloride solution again, produce the salt tolerant bacterium culture medium nutritional agents.
The present invention also provides a kind of method that Facultative Halophiles are tamed according to above-mentioned salt tolerant bacterium culture medium nutritional agents, including following step
Suddenly：
The salt tolerant bacterium culture medium nutritional agents of 30-60 parts is added in 100 parts of pure water, adds detesting for 3-10 parts
Oxygen animalcule, stir, Temperature fall after 35 DEG C of constant temperature is sealed by fermentation 24-48 hours；
The salt tolerant bacterium culture medium nutritional agents of 30-40 parts and the aerobic microbiological of 30-60 parts are added, is stirred,
30-40 DEG C of fermentation 12-24 hour of constant temperature, per 15-30 minutes oxygenic aeration once；Then, 25 DEG C of fermentation 8-12 hours of constant temperature, often
30-60 minutes oxygenic aeration is once；Last spontaneous fermentation 12-24 hours, per 120-240 minutes oxygenic aeration once；
It is naturally cooling to after being freeze-dried during normal temperature, refrigerated storage.
The present invention also provides another method that Facultative Halophiles are tamed according to above-mentioned salt tolerant bacterium culture medium nutritional agents, including following
Step：
The salt tolerant bacterium culture medium nutritional agents of 0.5-3 parts is added in 100 parts of pure water, adds detesting for 3-10 parts
Oxygen animalcule, stir, Temperature fall after 35 DEG C of constant temperature is sealed by fermentation 24-48 hours；
The salt tolerant bacterium culture medium nutritional agents of 30-40 parts and the aerobic microbiological of 3-10 parts are added, is stirred, it is permanent
Warm 30-40 DEG C of fermentation 12-24 hour, per 15-30 minutes oxygenic aeration once；Then, 25 DEG C of fermentation 8-12 hours of constant temperature, often
30-60 minutes oxygenic aeration is once；Last spontaneous fermentation 12-24 hours, per 120-240 minutes oxygenic aeration once；
It is naturally cooling to after being freeze-dried during normal temperature, refrigerated storage.
In above-mentioned acclimation method, the anaerobe and aerobic microbiological include but is not limited to：Bacillus subtilis,
Clothing bacillus, Bacillus natto, bacillus laterosporus, bacillus megaterium, bacillusmusilaginosiengineering, photosynthetic bacteria, more viscous gemma
Bacillus, bacillus coagulans, soil bacillus brevis, saccharomycete, EM bacterium, streptococcus fecalis, ocean rhodotorula, bacteriophagic Bdellovibrio,
Bacillus cereus, lactic acid bacteria, Lactobacillus plantarum, lactobacillus acidophilus, nitrobacteria, denitrifying bacteria, actinomyces, desulfurization arc
Bacterium, black-koji mould, aspergillus oryzae and trichoderma viride.
Present invention also offers a kind of application of above-mentioned salt tolerant bacterium culture medium nutritional agents in terms of Facultative Halophiles domestication and high salt
Application in terms of wastewater treatment.
Compared with prior art, salt tolerant bacterium culture medium nutritional agents of the invention can tame outstanding more Facultative Halophiles, Ke Yiyong
In bacillus subtilis, bacillus licheniformis, Bacillus natto, bacillus laterosporus, bacillus megaterium, bacillusmusilaginosiengineering,
Photosynthetic bacteria, bacillus polymyxa, bacillus coagulans, soil bacillus brevis, saccharomycete, EM bacterium, streptococcus fecalis, ocean are red
Yeast, bacteriophagic Bdellovibrio, bacillus cereus, lactic acid bacteria, Lactobacillus plantarum, lactobacillus acidophilus, nitrobacteria, denitrification are thin
The salt tolerant domestication of bacterium, actinomyces, desulfovibrio, black-koji mould, aspergillus oryzae, trichoderma viride etc., so as to meet high-salt wastewater
The process demand of complex environment, reach more preferable clean-up effect and stability.
The raw material of this salt tolerant bacterium culture medium nutritional agents is easy to get, and technological process is simple, can be to strain commercially
Microbial inoculum, directly tame, simple to operate, cost is low.
Embodiment
With reference to embodiment, the present invention is further illustrated.
The present invention discloses a kind of salt tolerant bacterium culture medium nutritional agents, including following parts by weight：Water 30000-50000 parts, sea
It is mosanom 8000-12000 parts, Kelp Powder 18000-22000 parts, fish meal 18000-22000 parts, dregs of beans 8000-12000 parts, swollen
Change bean powder 8000-12000 parts, yeast 4000-6000 parts, sargassum 8000-12000 parts, spirulina 4000-6000 parts, dimension life
Plain C 30-44 parts, Choline Chloride 20-30 parts, nicotinic acid 4.5-7.5 parts, calcium pantothenate 4-6 parts, vitamine D3 2.5-3.5 parts, dimension life
Plain E 4-6 parts, inositol 4-5 parts, vitamin A 5-7 parts, the 0.5-1.5 parts of biotin 2, vitamin B1 1.5-2.5 parts, dimension life
Plain B2 1.5-2.5 parts, vitamin B12 0.4-0.6 parts, Vitamin K3 0.6-1.4 parts, SPP1 powder 600-1000 parts, phosphoric acid
Hydrogen calcium 880-1320 parts, flour 16000-20000 parts, dry ooze 6000-8000 parts and 520-560 parts mineral trace element gold
Belong to salt compound.
Wherein, preferably 544.86 parts of mineral trace element metal salt compound, are specifically included：Magnesium sulfate monohydrate
(MgSO4H2O) 433.3 parts, 76.7 parts of ferrous sulfate monohydrate (FeSO4H2O), copper sulfate monohydrate (CuSO4H2O) 7.1
Part, 17.1 parts of monohydrate zinc sulphate (ZnSO4H2O), 10.1 parts of manganese sulfate monohydrate (MnSO4H2O), sulfuric acid monohydrate cobalt
(CoSO4H2O) 0.3 part and 0.26 part of KI (KI).
The preparation method of the salt tolerant bacterium culture medium nutritional agents of the present invention, comprises the following steps：
(1) it is, first that sodium alginate is stand-by with water-solubleization；
(2), Kelp Powder, fish meal, dregs of beans, expanded bean powder, yeast, sargassum, spirulina, dry ooze are mixed crushing and treated
With；
(3), vitamin C and Choline Chloride SPP1 powder are diluted stand-by；
(4), by nicotinic acid, calcium pantothenate, dimension ox element D3, vitamin E, inositol, Wei Shengsuo A, Wei Shengsuo H2, vitamin B1, dimension
Raw plain B2, vitamin B6, vitamin B12 and Vitamin K3 SPP1 powder dilute stand-by；
(5), mineral trace element metal salt compound is diluted with calcium monohydrogen phosphate stand-by；
(6), flour and step (2)~(5) stand-by raw material are mixed together uniformly, being further crushed to pass through
80-200 mesh sieves；
(7), stand-by solution prepared by step (1) is added in the powder obtained to step (6) and stirred and evenly mixed；
(8) drained after, being soaked 30 seconds with 5% calcium chloride solution again, produce the salt tolerant bacterium culture medium nutritional agents.
The salt tolerant bacterium culture medium nutritional agents can be used for directly taming a variety of anaerobic bacterias and aerobic bacteria, and anaerobe becomes reconciled
Oxygen animalcule includes but is not limited to：Bacillus subtilis, bacillus licheniformis, Bacillus natto, bacillus laterosporus, huge gemma bar
Bacterium, bacillusmusilaginosiengineering, photosynthetic bacteria, bacillus polymyxa, bacillus coagulans, soil bacillus brevis, saccharomycete, EM
Bacterium, streptococcus fecalis, ocean rhodotorula, bacteriophagic Bdellovibrio, bacillus cereus, lactic acid bacteria, Lactobacillus plantarum, lactobacillus acidophilus,
Nitrobacteria, denitrifying bacteria, actinomyces, desulfovibrio, black-koji mould, aspergillus oryzae and trichoderma viride.
Anaerobic bacteria anaerobic fermentation breeding domestication in the Facultative Halophiles acclimation method of the present invention, the domestication of aerobic bacteria aerated fermentation, tool
Body is as follows：
Using the freeze-drying of solid state fermentation final products into the Facultative Halophiles acclimation method of pulvis, comprise the following steps：
The salt tolerant bacterium culture medium nutritional agents of 30-60 parts is added in 100 parts of pure water, adds detesting for 3-10 parts
Oxygen animalcule, stir, Temperature fall after 35 DEG C of constant temperature is sealed by fermentation 24-48 hours；
The salt tolerant bacterium culture medium nutritional agents of 30-40 parts and the aerobic microbiological of 30-60 parts are added, is stirred,
30-40 DEG C of fermentation 12-24 hour of constant temperature, per 15-30 minutes oxygenic aeration once；Then, 25 DEG C of fermentation 8-12 hours of constant temperature, often
30-60 minutes oxygenic aeration is once；Last spontaneous fermentation 12-24 hours, per 120-240 minutes oxygenic aeration once；
It is naturally cooling to after being freeze-dried during normal temperature, refrigerated storage.Pay attention to：10 meter half of periphery span during culture
From forbidding to dial mobile phone.
Using the freeze-drying of liquid state fermentation final products into the Facultative Halophiles acclimation method of pulvis, comprise the following steps：
The salt tolerant bacterium culture medium nutritional agents of 0.5-3 parts is added in 100 parts of pure water, adds detesting for 3-10 parts
Oxygen animalcule, stir, Temperature fall after 35 DEG C of constant temperature is sealed by fermentation 24-48 hours；
The salt tolerant bacterium culture medium nutritional agents of 30-40 parts and the aerobic microbiological of 3-10 parts are added, is stirred, it is permanent
Warm 30-40 DEG C of fermentation 12-24 hour, per 15-30 minutes oxygenic aeration once；Then, 25 DEG C of fermentation 8-12 hours of constant temperature, often
30-60 minutes oxygenic aeration is once；Last spontaneous fermentation 12-24 hours, per 120-240 minutes oxygenic aeration once；
It is naturally cooling to after being freeze-dried during normal temperature, refrigerated storage.Pay attention to：10 meter half of periphery span during culture
From forbidding to dial mobile phone.
By the salt tolerant bacterium culture medium nutritional agents of following proportioning, pass through the reality of above-mentioned solid state fermentation acclimation method acquisition Facultative Halophiles
It is as follows to test data：
From the foregoing, it will be observed that the Facultative Halophiles tamed by salt tolerant bacterium culture medium nutritional agents of the present invention, 15% is up in salt content
Also there is very fast reproduction speed, so that stabilization can be played in hypersaline environment and good clean-up effect in environment.
During high-salt wastewater handles practical application, the biochemical anaerobic pond and Aerobic Pond of high-salt wastewater processing pond, in advance
Nutriment brown sugar, starch, urea and the potassium dihydrogen phosphate of routine are launched in proportion, BOD is pressed in anaerobic pond:N:P=350:
5:1 is calculated, and BOD is pressed in Aerobic Pond:N:P=100:5:1, or by Elements C:N:P=100:5：1 adds, according to flour, urine
Element, the C in potassium dihydrogen phosphate, N, P amounts calculate.After knowing BOD concentration, according to the dischargeable capacity in pond, dischargeable capacity is calculated
In BOD total amounts, being calculated further according to aforementioned proportion formula needs the N amounts, the P amounts that add, further according to containing for urea and potassium dihydrogen phosphate
Nitrogen quantity and phosphorus content can obtain the amount for needing the urea that adds and potassium dihydrogen phosphate, if it is known that the residence time, with adding
Amount divided by residence time can also obtain the amount of the urea that should be added per hour within the residence time and potassium dihydrogen phosphate.
Culture medium nutritional agents formula material is added again, is launched in the ratio of 10-35% in Aerobic Pond and anaerobic pond.Point
Anaerobism Facultative Halophiles are not added toward bio anaerobic pond, aerobic Facultative Halophiles are added in aerobic aeration pond.Add ratio:Thrown by 1-10 cubes of water
The ratio of 1 kilogram of sewage special bacteria is put, by the disposable all uniform input aeration tank of microbial inoculum, ratio can be according to sewage situation
Appropriate increase and decrease.Dosage is determined according to different types of waste water, technique, concentration, wastewater flow rate.
Wherein, water temperature cannot be below 15 degree, preferably between 20-30 degree.If 10-14 degree, 1 kilogram of sewage treatment microbial inoculant
Handle 1 side's sewage；15-19 degree, 1 kilogram of sewage treatment microbial inoculant handle 2 side's sewage；20-25 degree, at 1 kilogram of sewage treatment microbial inoculant
Manage 5 side's sewage；More than 25 degree, 1 kilogram of sewage treatment microbial inoculant handles 10 side's sewage；Water temperature can not surpass 45 degree.
Wherein, persistently it is aerated 24 hours, activates microorganism, attached work bacterium bed is simultaneously bred, and reaches active state.Using
Stage adjustment water inlet, to reduce the impact to microorganism, operation opens the 1/3 of normal inflow for first day, opens within second day
2/3, the 3rd day can standard-sized sheet.As inflow design it is less than normal, then can disposable standard-sized sheet.Monitoring is run with adaption systems, about 30 days
If system is stable afterwards, without adding microbial inoculum again.
In practical application, it is necessary to reference to sewage treatment process, therefore in any case, can be used biological reinforced
Technology：
1), during system carries out Start-up and Adjustment (culture domesticated organism strain)；
2) thing impact load, is contaminated during system operation, causes the comprehensive treatment capability of system to decline, it is impossible to be steady
When handling waste water surely；
3) when, re-starting system startup optimization after system of short duration (being usually no more than 72 hours) out of service；
4), system winter is out of service, in the new year temperature rise Start-up and Adjustment；
5), system is due to when polluter mutation sludge poisoning treatment effect declines.
Facultative Halophiles feature is obtained by the present invention：
1.pH values：Average range, can be to fast-growth between 6.6~7.4 between 5.5~9.5.
2. temperature：It can be come into force between 10 DEG C~60 DEG C.Temperature is higher than 60 DEG C, can cause the death of bacterium；Temperature is less than 10
DEG C when bacterium will not be dead, but its cell growth can be very restricted.It it is 26~32 DEG C to suitable temperature.
3. dissolved oxygen：Aeration tank in sewage disposal, dissolved oxygen amount are at least up to 2 mg/litres；The bacterium of contained high adaptive faculty
Race will accelerate 5~7 times in the metabolism to target substance and degradation speed in the oxygen of abundance.It is appropriate to be needed in soil remediation
Porous soil or ventilation oxygenating.
4. trace element：Proprietary bacterium race may require that many elements, such as potassium, iron, calcium, sulphur, magnesium in its growth, generally
Enough elements more than all containing in soil and water source.
5. salinity：It is to tell on that salinity, which is less than 0.5% directly to launch, realizes homeostasis and expands numerous；Salinity 0.5%-
About need domestication in 2-10 days to adapt to the water quality between 2%, realize homeostasis and expand numerous；About needed between salinity 2%-10%
Domestication in 10-30 days adapts to the water quality, realizes homeostasis and expands numerous
6. mithridatism：Chemotoxic substances, including chloride and heavy metal etc. can relatively efficiently be resisted.
The present invention is safe efficient, nontoxic, non-secondary pollution water treatment agent.The present invention can also improve sludge biochemical
Energy and flocculating effect, the settling ratio of sludge is effectively maintained, extend sludge age.The present invention can also effectively eliminate sludge bulking,
Sludge aging phenomenon is prevented, reduces sludge quantity, reduces Treatment of Sludge cost.The present invention is favorably improved COD, ammonia nitrogen removal frank,
With poisonous and harmful substance degradation capability.The present invention can control the peculiar smell in sewerage, and strong impact load and toxicity are supported
Imperial ability.
The present invention possesses the polluters such as superpower removal BOD, COD, SS, ammonia nitrogen, phosphorus, and effective percentage reaches more than 90-95%.This
Invention can directly reach country-level A standards or relevant criterion.It disposably puts into, without persistently adding bacterium after system is stable
Kind, pollution treatment cost is greatly reduced.
Present invention reply dyestuff and dyeing and finishing wastewater and other waste water with difficult elimination color, dispensing can direct bleaching.This
Invention is also equipped with significant deodorizing effect, and the ability for eliminating NK3, P, H2S and organic acid is superpower.
The present invention has superpower breeding and adaptability, gene upgrading, can tackle the Sewage Environment of following complexity, can drop
Solve the synthesis chemical combination pollutant such as agricultural chemicals, Polychlorinated biphenyls, plasticiser, synthetic detergent, biosynthesis plastics.
The present invention can also suppress virus, germ and parasite, suppress algae reproduction, purifying water body and water colour, remove life
Heavy metal pollution in sewage, such as zinc, manganese, iron, chromium ....
The present invention easily cultivates, breeding is fast, has the characteristics such as stronger adaptability and natural evolution to environment, once appearance is new
Pollution compound, they can also have stepped through spontaneous or induction and produce new enzyme system, possess new metabolic function, so as to degrade
Or the compound that conversion is new.
The present invention should be sealed and stored at shady and cool, drying, not deposited together with poisonous substance.
Obviously, the above embodiment of the present invention is only intended to clearly illustrate example of the present invention, and is not pair
The restriction of embodiments of the present invention.For those of ordinary skill in the field, may be used also on the basis of the above description
To make other changes in different forms.There is no necessity and possibility to exhaust all the enbodiments.It is all this
All any modification, equivalent and improvement made within the spirit and principle of invention etc., should be included in the claims in the present invention
Protection domain within.
Claims (10)
1. a kind of salt tolerant bacterium culture medium nutritional agents, it is characterised in that including following parts by weight：Water 30000-50000 parts, marine alga
It is sour sodium 8000-12000 parts, Kelp Powder 18000-22000 parts, fish meal 18000-22000 parts, dregs of beans 8000-12000 parts, expanded
Bean powder 8000-12000 parts, yeast 4000-6000 parts, sargassum 8000-12000 parts, spirulina 4000-6000 parts, vitamin C
30-44 parts, Choline Chloride 20-30 parts, nicotinic acid 4.5-7.5 parts, calcium pantothenate 4-6 parts, vitamine D3 2.5-3.5 parts, vitamin E
4-6 parts, inositol 4-5 parts, vitamin A 5-7 parts, the 0.5-1.5 parts of biotin 2, vitamin B1 1.5-2.5 parts, vitamin B2
1.5-2.5 parts, vitamin B12 0.4-0.6 parts, Vitamin K3 0.6-1.4 parts, SPP1 powder 600-1000 parts, calcium monohydrogen phosphate
880-1320 parts, flour 16000-20000 parts, dry ooze 6000-8000 parts and 520-560 part mineral trace element metal salts
Compound.
2. salt tolerant bacterium culture medium nutritional agents according to claim 1, it is characterised in that the mineral trace element metal
Salt compound includes：433.3 parts of magnesium sulfate monohydrate, 76.7 parts of ferrous sulfate monohydrate, 7.1 parts of copper sulfate monohydrate, monohydrate zinc sulphate
0.26 part of 17.1 parts, 10.1 parts of manganese sulfate monohydrate, 0.3 part of sulfuric acid monohydrate cobalt and KI.
3. salt tolerant bacterium culture medium nutritional agents according to claim 2, it is characterised in that including following parts by weight：Water
50000 parts, 10000 parts of sodium alginate, 20000 parts of Kelp Powder, 20000 parts of fish meal, 10000 parts of dregs of beans, expanded bean powder 10000
It is part, 5000 parts of yeast, 10000 parts of sargassum, 5000 parts of spirulina, 36 parts of vitamin C, 26 parts of Choline Chloride, 6 parts of nicotinic acid, general
Sour 5 parts of calcium, 3 parts of vitamine D3,5 parts of vitamin E, 4.5 parts of inositol, 6 parts of vitamin A, 21 parts of biotin, vitamin B1 2
Part, 2 parts of vitamin B2,0.5 part of vitamin B12,1 part of Vitamin K3,800 parts of SPP1 powder, 433.3 parts of magnesium sulfate monohydrate,
76.7 parts of ferrous sulfate monohydrate, 7.1 parts of copper sulfate monohydrate, 17.1 parts of monohydrate zinc sulphate, 10.1 parts of manganese sulfate monohydrate, sulfuric acid monohydrate
8000 parts of 0.3 part of cobalt, 0.26 part of KI, 1000 parts of calcium monohydrogen phosphate, 18000 parts of flour and dry ooze.
4. salt tolerant bacterium culture medium nutritional agents according to claim 2, it is characterised in that including following parts by weight：Water
40000 parts, 8000 parts of sodium alginate, 22000 parts of Kelp Powder, 18000 parts of fish meal, 8000 parts of dregs of beans, 12000 parts of expanded bean powder,
It is 4000 parts of yeast, 12000 parts of sargassum, 6000 parts of spirulina, 44 parts of vitamin C, 30 parts of Choline Chloride, 7.5 parts of nicotinic acid, general
Sour 6 parts of calcium, 3.5 parts of vitamine D3,6 parts of vitamin E, 5 parts of inositol, 7 parts of vitamin A, 2 1.5 parts of biotin, vitamin B1
2.5 parts, 2.5 parts of vitamin B2,0.6 part of vitamin B12,1.4 parts of Vitamin K3,1000 parts of SPP1 powder, magnesium sulfate monohydrate
433.3 parts, 76.7 parts of ferrous sulfate monohydrate, 7.1 parts of copper sulfate monohydrate, 17.1 parts of monohydrate zinc sulphate, 10.1 parts of manganese sulfate monohydrate,
7000 parts of 0.3 part of sulfuric acid monohydrate cobalt, 0.26 part of KI, 880 parts of calcium monohydrogen phosphate, 20000 parts of flour and dry ooze.
5. salt tolerant bacterium culture medium nutritional agents according to claim 2, it is characterised in that including following parts by weight：Water
30000 parts, 12000 parts of sodium alginate, 18000 parts of Kelp Powder, 22000 parts of fish meal, 12000 parts of dregs of beans, 8000 parts of expanded bean powder,
6000 parts of yeast, 8000 parts of sargassum, 4000 parts of spirulina, 30 parts of vitamin C, 20 parts of Choline Chloride, 4.5 parts of nicotinic acid, pantothenic acid
4 parts of calcium, 2.5 parts of vitamine D3,4 parts of vitamin E, 4 parts of inositol, 5 parts of vitamin A, 2 0.5 parts of biotin, vitamin B1
1.5 parts, 1.5 parts of vitamin B2,0.4 part of vitamin B12,0.6 part of Vitamin K3,600 parts of SPP1 powder, magnesium sulfate monohydrate
433.3 parts, 76.7 parts of ferrous sulfate monohydrate, 7.1 parts of copper sulfate monohydrate, 17.1 parts of monohydrate zinc sulphate, 10.1 parts of manganese sulfate monohydrate,
6000 parts of 0.3 part of sulfuric acid monohydrate cobalt, 0.26 part of KI, 1320 parts of calcium monohydrogen phosphate, 16000 parts of flour and dry ooze.
A kind of 6. preparation method of salt tolerant bacterium culture medium nutritional agents as described in any one of claim 1 to 5, it is characterised in that
Comprise the following steps：
(1) it is, first that sodium alginate is stand-by with water-solubleization；
(2), Kelp Powder, fish meal, dregs of beans, expanded bean powder, yeast, sargassum, spirulina, the mixing of dry ooze are crushed stand-by；
(3), vitamin C and Choline Chloride SPP1 powder are diluted stand-by；
(4), by nicotinic acid, calcium pantothenate, dimension ox element D3, vitamin E, inositol, Wei Shengsuo A, Wei Shengsuo H2, vitamin B1, vitamin
B2, vitamin B6, vitamin B12 and Vitamin K3 SPP1 powder dilute stand-by；
(5), mineral trace element metal salt compound is diluted with calcium monohydrogen phosphate stand-by；
(6), flour and step (2)~(5) stand-by raw material are mixed together uniformly, 80- can be passed through by being further crushed to
200 mesh sieves；
(7), stand-by solution prepared by step (1) is added in the powder obtained to step (6) and stirred and evenly mixed；
(8) drained after, being soaked 30 seconds with 5% calcium chloride solution again, produce the salt tolerant bacterium culture medium nutritional agents.
7. a kind of method of the domestication Facultative Halophiles of salt tolerant bacterium culture medium nutritional agents according to any one of claim 1 to 5, its
It is characterised by, comprises the following steps：
The salt tolerant bacterium culture medium nutritional agents of 30-60 parts is added in 100 parts of pure water, the anaerobism for adding 3-10 parts is micro-
Biology, stir, Temperature fall after 35 DEG C of constant temperature is sealed by fermentation 24-48 hours；
The salt tolerant bacterium culture medium nutritional agents of 30-40 parts and the aerobic microbiological of 30-60 parts are added, is stirred, constant temperature
30-40 DEG C of fermentation 12-24 hour, per 15-30 minutes oxygenic aeration once；Then, 25 DEG C of fermentation 8-12 hours of constant temperature, per 30-
60 minutes oxygenic aerations are once；Last spontaneous fermentation 12-24 hours, per 120-240 minutes oxygenic aeration once；
It is naturally cooling to after being freeze-dried during normal temperature, refrigerated storage.
8. a kind of method of the domestication Facultative Halophiles of salt tolerant bacterium culture medium nutritional agents according to any one of claim 1 to 5, its
It is characterised by, comprises the following steps：
The salt tolerant bacterium culture medium nutritional agents of 0.5-3 parts is added in 100 parts of pure water, the anaerobism for adding 3-10 parts is micro-
Biology, stir, Temperature fall after 35 DEG C of constant temperature is sealed by fermentation 24-48 hours；
The salt tolerant bacterium culture medium nutritional agents of 30-40 parts and the aerobic microbiological of 3-10 parts are added, is stirred, constant temperature
30-40 DEG C of fermentation 12-24 hour, per 15-30 minutes oxygenic aeration once；Then, 25 DEG C of fermentation 8-12 hours of constant temperature, per 30-
60 minutes oxygenic aerations are once；Last spontaneous fermentation 12-24 hours, per 120-240 minutes oxygenic aeration once；
It is naturally cooling to after being freeze-dried during normal temperature, refrigerated storage.
9. method as claimed in claim 7 or 8, it is characterised in that the anaerobe and aerobic microbiological are included but not
It is limited to：Bacillus subtilis, bacillus licheniformis, Bacillus natto, bacillus laterosporus, bacillus megaterium, gel-shaped gemma bar
Bacterium, photosynthetic bacteria, bacillus polymyxa, bacillus coagulans, soil bacillus brevis, saccharomycete, EM bacterium, streptococcus fecalis, sea
Fuchsin yeast, bacteriophagic Bdellovibrio, bacillus cereus, lactic acid bacteria, Lactobacillus plantarum, lactobacillus acidophilus, nitrobacteria, denitrification
Bacterium, actinomyces, desulfovibrio, black-koji mould, aspergillus oryzae and trichoderma viride.
10. application and high salt of the salt tolerant bacterium culture medium nutritional agents described in any one of claim 1 to 5 in terms of Facultative Halophiles domestication
Application in terms of wastewater treatment.
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