CN103820378A - Method for acclimatizing animal derived microorganisms for sewage purification into plant derived microorganisms - Google Patents
Method for acclimatizing animal derived microorganisms for sewage purification into plant derived microorganisms Download PDFInfo
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Abstract
The invention discloses a method for acclimatizing animal derived microorganisms for sewage purification into plant derived microorganisms. The method comprises the following steps: adding 30-40 parts of plant derived culture medium into 100 parts of pure water, then, adding 3-10 parts of anaerobic microorganisms, uniformly stirring, and carrying out sealed fermentation for 24-48 hours at the constant temperature of 35 DEG C; then, adding 30-40 parts of plant derived culture medium and 3-10 parts of aerobic microorganisms, uniformly stirring, and carrying out fermentation for 12-24 hours at the constant temperature of 30 DEG C, wherein oxygenating for aeration is carried out once every 15-30 minutes; then, carrying out fermentation for 8-12 hours at the constant temperature of 25 DEG C, wherein oxygenating for aeration is carried out once every 30-60 minutes; finally, carrying out natural fermentation for 12-24 hours, wherein oxygenating for aeration is carried out once every 120-240 minutes, wherein the weight part ratio of anaerobic microorganisms to aerobic microorganisms is 1: 1. According to the method, the animal derived microorganisms purchased are acclimatized, and the formula can be adjusted flexibly according to the actual circumstances of different sewages, so as to recover the original wild nature of the microorganisms and enable the microorganisms to quickly survive, accommodate and propagate; the requirements on the environment are low, the operation is convenient, the cost is low, and the effect taking speed is high.
Description
Technical field
The present invention relates to microorganism sewage purifying agent, be specifically related to sewage purification animal source microbial acclimation and become plant-sourced method of microorganism.
Background technology
Organic matter in microbes water of decomposition and inanimate matter use as self nutrient, thereby reach the effect purifying water, and adopt microbial technique to carry out purification of water quality and can not produce secondary environmental pollution, are therefore state-of-the-art water purification technologies in the world at present.But the microorganism that existing microorganism sewage purifying agent is used is all cultivated acquisition by zoogenous substratum, this animal source microorganism is for the very severe physical environment in field, and effect is also bad or not obvious, and major cause is:
(1) use peptone, extractum carnis etc. as substratum, cost is very high, and need to carry out at sterilized space, complicated operation.
(2) animal source microorganism is high to the temperature requirement of culture environment, and temperature is low cannot cultivate.
In view of this, the animal source microorganism that urgent need will be used existing microorganism water quality cleansing agent is tamed and dociled plant-sourced microorganism, to improve its microorganism active height and adaptation vigor.
Summary of the invention
Technical problem to be solved by this invention is that the animal source microorganism culturing cost for microorganism sewage purifying agent is high, activity and the poor problem of adaptation vigor.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is to provide a kind of sewage purification animal source microbial acclimation and becomes plant-sourced method of microorganism, comprises the following steps:
In the pure water of 100 parts, add the plant-sourced substratum of 30-40 part, then the anaerobion that adds 3-10 part, stir, 35 ℃ of sealing and fermenting of constant temperature 24-48 hours;
Add again the plant-sourced substratum of 30-40 part and the aerobic microbiological of 3-10 part, stir, 30 ℃ of fermentation 12-24 hour of constant temperature, every 15-30 minute oxygenic aeration is once; Then, 25 ℃ of fermentation 8-12 hour of constant temperature, every 30-60 minute oxygenic aeration is once; Last spontaneous fermentation 12-24 hour, every 120--240 minute oxygenic aeration is once;
Wherein, the ratio of weight and number of anaerobion and aerobic microbiological is 1:1.
In aforesaid method, the plant-sourced substratum in described plant-sourced nutrient solution is made up of the component of following parts by weight:
Plant-derived protein 85-98%;
Plant-sourced high-energy polysaccharose substance 0.8-14.5%;
Sodium-chlor powder 0.5-3%.
In aforesaid method, every 100 grams of described plant-sourced nutrient solutions comprise:
Protein: 1.8-18 gram;
Carbohydrate: 1.10-11 gram;
Fat: 0.7-7 gram;
Mierocrystalline cellulose: 1.10-11 gram;
Trace element: 0.1-0.5 gram;
All the other are pure water.
In aforesaid method, the contained described trace element of every 100 grams of plant-sourced nutrient solutions comprises:
Vitamin A: 15-150 microgram;
Vitamin-E: 0.8-10 milligram;
Carotene: 90-900 microgram;
Magnesium: 10-45 milligram;
Calcium: 10-50 milligram;
Iron: 0.5-2.5 milligram;
Zinc: 0.24-1.2 milligram;
Copper: 0.07-0.35 milligram;
Manganese: 0.09-0.45 milligram;
Potassium: 48-240 milligram;
Phosphorus: 30-150 milligram.
In aforesaid method, described plant-sourced high-energy polysaccharose substance comprises brown sugar, glucose, fructose, any one or several mixture in multiple monose and polysaccharide fiber element, hemicellulose, pectin substance, xylogen and starch, and blending ratio is any.
In aforesaid method, described anaerobion and aerobic microbiological include but not limited to: subtilis, Bacillus licheniformis, Bacillus natto, bacillus laterosporus, bacillus megaterium, bacillusmusilaginosiengineering, photosynthetic bacterium, bacillus polymyxa, Bacillus coagulans, soil bacillus brevis, yeast, EM bacterium, streptococcus faecium, ocean rhodotorula, bacteriophagic Bdellovibrio, bacillus cereus, milk-acid bacteria, plant lactobacillus, Lactobacterium acidophilum, nitrobacteria, denitrifying bacterium, actinomycetes and microorganism fermentating metabolism product enzyme, peptide, amino acid, organic acid, microbiotic
The present invention, tame buying the animal source microbial strains coming, recover the original wild nature of microorganism (the very strong survival activity conforming rapidly), not only make its vigor strengthen, and reform of nature circle (the particularly severe environment in sewage disposal) faster, survive fast, adapt to, expand numerous, and low to environmental requirement, easy to operate.
Embodiment
In the present invention, animal source microorganism refer to the animal source such as extractum carnis, peptone be main culture medium culturing microbial strains out; Plant-sourced microorganism refer to plant-sourced vegetable-protein, plant-sourced polyose etc. be main culture medium culturing microbial strains out.
Nowadays the microbial strains that, purification of water quality adopts generally includes two kinds of anaerobion and aerobic microbiologicals.But the mentioned microorganism bacterial classification of market sale is all animal source microbial strains, in very severe physical environment, effect is bad or not obvious in the wild.The present invention, tame buying the animal source microbial strains coming, recover the original wild nature of microorganism (the very strong survival activity conforming rapidly), not only make its vigor strengthen, and reform of nature environment (the particularly severe environment of sewage disposal) faster.
Below in conjunction with specific embodiment, the present invention is described in detail.
Sewage purification provided by the invention becomes plant-sourced method of microorganism with animal source microbial acclimation, utilize plant-sourced nutrient solution that the fermentation of animal source microorganism is domesticated for to plant-sourced microorganism, wherein, animal source microorganism is 1:1 by ratio of weight and number anaerobion and aerobic microbiological form.Microorganism includes but not limited to animal source: subtilis, Bacillus licheniformis, Bacillus natto, bacillus laterosporus, bacillus megaterium, bacillusmusilaginosiengineering, photosynthetic bacterium, bacillus polymyxa, Bacillus coagulans, soil bacillus brevis, yeast, EM bacterium, streptococcus faecium, ocean rhodotorula, bacteriophagic Bdellovibrio, bacillus cereus, milk-acid bacteria, plant lactobacillus, Lactobacterium acidophilum, nitrobacteria, denitrifying bacterium, actinomycetes and microorganism fermentating metabolism product enzyme (amylase, beta-glucanase, saccharifying enzyme, proteolytic enzyme, cellulase, hemicellulase, polygalacturonase, lipase, phytase, M-Zyme, zytase), peptide (polypeptide active peptide, large peptide), amino acid, organic acid and microbiotic.
Plant-sourced substratum (Powdered) in plant-sourced nutrient solution comprises the component of following parts by weight:
Plant-derived protein 85-98%;
Plant-sourced high-energy polysaccharose substance 0.8-14.5%;
Sodium-chlor powder 0.5-3%.
In several, conventional proportioning is as following table:
Embodiment 1 embodiment 2 embodiment 3
Plant-derived protein 85 98 92
Plant-sourced high-energy polyose thing 14.5 0.8 5
Sodium-chlor powder 0.5 1.2 3
Wherein, every 100 grams of plant-sourced nutrient solutions contain:
Protein: 1.8-18 gram;
Carbohydrate: 1.10-11 gram;
Fat: 0.7-7 gram;
Mierocrystalline cellulose: 1.10-11 gram;
Trace element: 0.1-0.5 gram;
All the other are pure water.
Micro-is specifically composed as follows.Every 100 grams of contained trace elements of plant-sourced culture medium culturing liquid comprise:
Vitamin A: 15-150 microgram;
Vitamin-E: 0.8-10 milligram;
Carotene: 90-900 microgram;
Magnesium: 10-45 milligram;
Calcium: 10-50 milligram;
Iron: 0.5-2.5 milligram;
Zinc: 0.24-1.2 milligram;
Copper: 0.07-0.35 milligram;
Manganese: 0.09-0.45 milligram;
Potassium: 48-240 milligram;
Phosphorus: 30-150 milligram.
Plant-sourced high-energy polysaccharose substance comprises brown sugar, glucose, fructose, any one or several mixture in multiple monose and polysaccharide fiber element, hemicellulose, pectin substance, xylogen and starch, and blending ratio is any.
Several typical composition composition embodiment of every 100 grams of plant-sourced nutrient solutions is as following table:
Utilize plant-sourced nutrient solution that the fermentation of animal source microorganism is domesticated for to plant-sourced method of microorganism as follows:
The domestication condition of A, plant-sourced microorganism:
1) 100 parts of pure water, the electric rate of conducting electricity is necessary for 0;
2) plant-sourced substratum 60-80 part, high temperature steaming sterilization in 30 minutes is in advance ready to;
3) animal source microorganism 6-20 part, anaerobism, aerobic microbiological are separately;
4) domestication environment, sealed vessel, UV-lamp that outside only needs to send out ozoniferous, simple, cost is low, without complicated sterilized space.
The domestication step of B, plant-sourced microorganism:
1) the plant-sourced substratum (this enforcement gets 1/3rd) of getting 30-40 parts by weight joins in the pure water of 100 parts, then the anaerobion that adds 3-10 part, stir, and 35 ℃ of sealing and fermenting of constant temperature 24-48 hours;
2) add again the plant-sourced substratum (this enforcement gets 1/3rd) of 30-40 and the aerobic microbiological of 3-10 part, stir, 30 ℃ of fermentation 12-24 hour of constant temperature, every 15-30 minute oxygenic aeration is once;
3) 25 ℃ of fermentation 8-12 hour of constant temperature, every 30-60 minute oxygenic aeration is once;
4) spontaneous fermentation 12-24 hour, every 120--240 minute oxygenic aeration once.
The plant-sourced microbiological property that the present invention obtains is as follows:
Effectively breeding rate: 5 × 10
9, 20,000,000,000/g;
Proterties: 1, powdery (greyish white), solvable; 2, fineness: 160 orders; PH value is at 2-5.5;
Environmental adaptation temperature:
| Index | Temperature (℃) |
| General propagation | +5℃～+85℃ |
| Best propagation | +25℃～+75℃ |
| Spore dormant state | +4℃～+86℃ |
| Damp and hot situation | －40℃～+119℃ |
| Without wet situation | －60℃～+280℃ |
Environment resistance to extreme temperature (spore type)
| Index | Resistance to extreme temperature |
| General breeding | More than 15% |
| Best breeding | 40%～100% |
| Spore state | Below 10% |
The present invention is not limited to above-mentioned preferred forms, and anyone should learn the structural changes of making under enlightenment of the present invention, and every have identical or close technical scheme with the present invention, within all falling into protection scope of the present invention.
Claims (6)
1. sewage purification becomes plant-sourced method of microorganism with animal source microbial acclimation, it is characterized in that, comprises the following steps:
In the pure water of 100 parts, add the plant-sourced substratum of 30-40 part, then the anaerobion that adds 3-10 part, stir, 35 ℃ of sealing and fermenting of constant temperature 24-48 hours;
Add again the plant-sourced substratum of 30-40 part and the aerobic microbiological of 3-10 part, stir, 30 ℃ of fermentation 12-24 hour of constant temperature, every 15-30 minute oxygenic aeration is once; Then, 25 ℃ of fermentation 8-12 hour of constant temperature, every 30-60 minute oxygenic aeration is once; Last spontaneous fermentation 12-24 hour, every 120--240 minute oxygenic aeration is once;
Wherein, the ratio of weight and number of anaerobion and aerobic microbiological is 1:1.
2. the method for claim 1, is characterized in that, the plant-sourced substratum in described plant-sourced nutrient solution is made up of the component of following parts by weight:
Plant-derived protein 85-98%;
Plant-sourced high-energy polysaccharose substance 0.8-14.5%;
Sodium-chlor powder 0.5-3%.
3. method as claimed in claim 2, is characterized in that, every 100 grams of described plant-sourced nutrient solutions comprise:
Protein: 1.8-18 gram;
Carbohydrate: 1.10-11 gram;
Fat: 0.7-7 gram;
Mierocrystalline cellulose: 1.10-11 gram;
Trace element: 0.1-0.5 gram;
All the other are pure water.
4. method as claimed in claim 3, is characterized in that, the contained described trace element of every 100 grams of plant-sourced nutrient solutions comprises:
Vitamin A: 15-150 microgram;
Vitamin-E: 0.8-10 milligram;
Carotene: 90-900 microgram;
Magnesium: 10-45 milligram;
Calcium: 10-50 milligram;
Iron: 0.5-2.5 milligram;
Zinc: 0.24-1.2 milligram;
Copper: 0.07-0.35 milligram;
Manganese: 0.09-0.45 milligram;
Potassium: 48-240 milligram;
Phosphorus: 30-150 milligram.
5. the method for claim 1, it is characterized in that, described plant-sourced high-energy polysaccharose substance comprises brown sugar, glucose, fructose, any one or several mixture in multiple monose and polysaccharide fiber element, hemicellulose, pectin substance, xylogen and starch, and blending ratio is any.
6. the method for claim 1, it is characterized in that, described anaerobion and aerobic microbiological include but not limited to: subtilis, Bacillus licheniformis, Bacillus natto, bacillus laterosporus, bacillus megaterium, bacillusmusilaginosiengineering, photosynthetic bacterium, bacillus polymyxa, Bacillus coagulans, soil bacillus brevis, yeast, EM bacterium, streptococcus faecium, ocean rhodotorula, bacteriophagic Bdellovibrio, bacillus cereus, milk-acid bacteria, plant lactobacillus, Lactobacterium acidophilum, nitrobacteria, denitrifying bacterium, actinomycetes and microorganism fermentating metabolism product enzyme, peptide, amino acid, organic acid and microbiotic.
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Cited By (12)
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| CN104278002A (en) * | 2014-10-14 | 2015-01-14 | 江门市地尔汉宇电器股份有限公司 | Compound microbial agent for urban sewage treatment, preparation method and application thereof |
| CN105063109A (en) * | 2015-09-02 | 2015-11-18 | 钟华 | Method for obtaining short-chain fatty acids by vegetable protein microbial fermentation |
| CN105177066A (en) * | 2015-09-02 | 2015-12-23 | 钟华 | Preparation method of short-chain fatty acid for deodorizing sewage and degrading sludge |
| CN105248883A (en) * | 2015-09-02 | 2016-01-20 | 钟华 | Method for degrading harmful residue in aquatic and poultry animal bodies by using short chain fatty acid |
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Cited By (15)
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| CN104278002A (en) * | 2014-10-14 | 2015-01-14 | 江门市地尔汉宇电器股份有限公司 | Compound microbial agent for urban sewage treatment, preparation method and application thereof |
| CN105248883B (en) * | 2015-09-02 | 2019-01-04 | 钟华 | A method of utilizing hazard residue in short-chain fat acid degradation aquatic products livestock and poultry animal body |
| CN105063109A (en) * | 2015-09-02 | 2015-11-18 | 钟华 | Method for obtaining short-chain fatty acids by vegetable protein microbial fermentation |
| CN105177066A (en) * | 2015-09-02 | 2015-12-23 | 钟华 | Preparation method of short-chain fatty acid for deodorizing sewage and degrading sludge |
| CN105248883A (en) * | 2015-09-02 | 2016-01-20 | 钟华 | Method for degrading harmful residue in aquatic and poultry animal bodies by using short chain fatty acid |
| CN105524860A (en) * | 2015-12-25 | 2016-04-27 | 杨泽科 | Culture method of fungicide for water purification |
| CN105850512A (en) * | 2016-06-03 | 2016-08-17 | 开县代祥食用菌种植厂 | Planting method for white mushrooms |
| CN107603939A (en) * | 2017-11-03 | 2018-01-19 | 钟华 | A kind of salt tolerant bacterium culture medium nutritional agents and preparation method thereof and acclimation method and application |
| CN107628668A (en) * | 2017-11-08 | 2018-01-26 | 钟华 | Mixed bacteria, strain carrier and the method for sustainable degraded creek black and odorous water petrochina pollutant |
| CN107673485A (en) * | 2017-11-08 | 2018-02-09 | 钟华 | Mixed bacteria, strain carrier and the method for excrement colibacillus group in sustainable processing creek black and odorous water |
| CN107619808A (en) * | 2017-11-08 | 2018-01-23 | 钟华 | Mixed bacteria, strain carrier and the method for anion surfactant in sustainable degraded creek black and odorous water |
| CN108503048A (en) * | 2018-05-07 | 2018-09-07 | 钟华 | A method of utilizing electricity-producing microorganism biofilm anode biology electrolytic cleaning creek black and odorous water |
| CN108503048B (en) * | 2018-05-07 | 2020-10-20 | 钟华 | Method for purifying river black and odorous water body by utilizing electrogenesis microorganism biofilm anode bioelectrolysis |
| CN112143770A (en) * | 2020-09-04 | 2020-12-29 | 清华苏州环境创新研究院 | Rhodotorula benthica and application thereof in production of beta-carotene by taking straws as raw material |
| CN112143770B (en) * | 2020-09-04 | 2023-09-22 | 清华苏州环境创新研究院 | Marine rhodotorula and application thereof in producing beta-carotene by taking straw as raw material |
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