CN105177066A - Preparation method of short-chain fatty acid for deodorizing sewage and degrading sludge - Google Patents
Preparation method of short-chain fatty acid for deodorizing sewage and degrading sludge Download PDFInfo
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- CN105177066A CN105177066A CN201510559352.5A CN201510559352A CN105177066A CN 105177066 A CN105177066 A CN 105177066A CN 201510559352 A CN201510559352 A CN 201510559352A CN 105177066 A CN105177066 A CN 105177066A
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Abstract
The invention discloses a preparation method of short-chain fatty acid for deodorizing sewage and degrading sludge. The preparation method comprises the following steps: grinding cereal and bean vegetable proteins into pulp, carrying out high-temperature disinfection, and cooling to 35-40 DEG C; carrying out fermentation and domestication on animal-based microbes by utilizing plant-based culture solution to form plant-based microbes, adding the plant-based microbes into the vegetable protein liquid, and carrying out mixed fermentation at 35-40 DEG C for 24-72h, thus obtaining the short-chain fatty acid; and mixing sodium alginate solution with concentration of 1%-5% with calcium chloride solution with concentration of 5%, and stirring the obtained solution with a mineralized water purificant loaded with the magnetotactic plant-based microbes and the short-chain fatty acid, thus forming a short-chain fatty acid microcapsule body. According to the preparation method, under the condition of guaranteeing the low cost, the short-chain fatty acid with high quality and high purity can be rapidly obtained; by utilizing the microencapsulation, the influences of heavy metals and other hazardous substances in river sludge on degrading of the sludge and denitrification, phosphate solubilizing and deodorizing of sewage by adopting short-chain fatty acid are avoided, thus the river purification efficiency is improved, the river self-cleaning capacity is promoted, and the use and operation are simple.
Description
Technical field
The present invention relates to technical field of microbial fermentation, be specifically related to the preparation method of a kind of sewage deodorizing degraded mud short chain fatty acid.
Background technology
Usually we are according to the number of carbon atom in carbochain, the organic aliphatic acid being 1-6 carbonatoms becomes short chain fatty acid (short-chainfattyacids, SCFA), also known as voltaile fatty acid (Volatilefattyacids, VFA), short chain fatty acid mainly comprises acetic acid, propionic acid, isopropylformic acid, butyric acid, isovaleric acid, valeric acid.
Short chain fatty acid SCFA can degrade the hazardous and noxious substances of a large amount of pathogenic agent, parasite, heavy metal element and some difficult degradations, and machine thing is changed into organic acid instead of GHG carbon dioxide and methane in degradation process, non-carbon-emitting, discharges without foul smell; Organic matter 80% left/right rotation of simultaneously degrading turns to solvable COD (ChemicalOxygenDemand, chemical oxygen demand (COD)), visible, short chain fatty acid SCFA and be suitable for sewage sludge denitrogenation phosphorus decomposing deodorizing.
At present, obtain short chain fatty acid SCFA by obtaining by anaerobion fermented sludge, this have following shortcoming with the short chain fatty acid SCFA that anaerobion fermented sludge obtains:
(1) microorganism of fermented sludge by the heavy metal, chemical organic compounds etc. in sewage sludge by harmful substances, and sewage, sludge quality, environmental factors (as temperature, pH, redox potential etc.), operating parameter (as hydraulic detention time, solid retention time etc.) impact very large, reduce the denitrogenation phosphorus decomposing odor removal efficient of sewage sludge;
(2) kind of mud, mud particle diameter, produce the formation that technology type that short chain fatty acid SCFAs adopts and reactor structure etc. can affect mud acidizing product short chain fatty acid SCFAs to a certain extent, cause and effectively cannot control the forming process of short chain fatty acid SCFAs, be unfavorable for that scale production is applied;
(3) the short chain fatty acid SCFAs impurity that obtains of anaerobion fermented sludge is many, is unfavorable for storage;
(4) in three stages of sludge anaerobic fermentation, in mud, to be hydrolyzed to the speed of dissolved matter slower for particulate organic matter matter, make the rate reduction of whole anaerobic fermentation process, in order to improve the efficiency of anaerobically fermenting, propose the method improving sludge hydrolytic speed, the ultimate principle of this method impels the organic substance decomposing of particulate form in fermented sludge to be the organism of small molecules and solubilised state, thus improve organic biodegradability; At present, the method improving sludge hydrolytic speed has thermal treatment, chemical method, mechanical process, oxidation style, biological process etc., and these method complicated operations, gigantic project, cost is high.
Summary of the invention
Technical problem to be solved by this invention be short chain fatty acid SCFA that existing method obtains exist affect greatly by extraneous factor, impurity is many, the not easily shortcoming such as storage, use under not only being suitable for sewage sludge environment, and there is the problems such as sludge degradation efficiency is low.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is to provide the preparation method of a kind of sewage deodorizing degraded mud short chain fatty acid, comprises the following steps:
The cereal beans vegetable-protein of 30-40 part is added in the pure water of 100 parts and grinds pulping, and sterilize under 90-120 degree high temperature, then cool the temperature to 35-40 degree;
Utilize plant-sourced nutrient solution that animal source fermentable is domesticated for plant-sourced microorganism, 1 part of plant-sourced microorganism is added in 100 parts of vegetable-protein liquid, uniform stirring, at 35-40 degree temperature lower seal fermentation 24-72 hour, obtain short chain fatty acid;
Be 1%-5% sodium alginate soln by concentration be that 5% calcium chloride solution mixes according to ratio of quality and the number of copies 1:1 with concentration, again above-mentioned mixing solutions, the mineralized water quality scavenging agent of load magnetotaxis plant-sourced microorganism and the short chain fatty acid of acquisition are mixed according to ratio of quality and the number of copies 1:1:100, uniform stirring, obtains short chain fatty acid microcapsules.
In the above-mentioned methods, described plant-sourced microorganism is anaerobion.
In the above-mentioned methods, according to microbial physiology metabolic function, the microorganism being domesticated for plant-sourced by animal source includes but not limited to:
Protein decomposing bacteria: clostridium butylicum;
Carbohydrate breakdown bacterium: yeast;
Steatolysis bacterium: streptococcus faecium, milk-acid bacteria, plant lactobacillus and Lactobacterium acidophilum;
Cellulose-decomposing bacterium: EM bacterium;
Hydrogen-producing acetogens and homoacetogenic bacteria.
The present invention utilizes plant-sourced microorganism to carry out anaerobically fermenting to cereal beans vegetable-protein, temperature, pH, redox potential and fermentation time when making anaerobically fermenting acquisition short chain fatty acid SCFAs can be constant, avoiding ectocine, obtaining rapidly high-quality, highly purified short chain fatty acid SCFAs when ensureing low cost; By microcapsulary, the mineralized water quality scavenging agent of short chain fatty acid SCFAs and load magnetotaxis plant-sourced microorganism is combined together simultaneously, in use, only directly need spread fertilizer over the fields input river to gush, not only make it sink to rapidly river to gush end mud and do not run off, use easy and simple to handle, and avoid river to gush Heavy Metals in Sludge and other objectionable impurities has an impact to chain fatty acids SCFAs, improve river and gush purification efficiency, promote that self-cleaning ability is gushed in river.
Embodiment
In the present invention, plant-sourced microorganism is utilized to carry out anaerobically fermenting to cereal beans vegetable-protein, and obtain a kind of sludge degradation short chain fatty acid SCFAs being applicable to sewage sludge environment by microcapsulary, not only degraded mud is gushed in the creationary river that is applied to by SCFAs, reaches the effect of denitrogenation phosphorus decomposing deodorizing; And utilize this short chain fatty acid SCFAs to carry out degraded river to gush mud, also have following benefit:
(1), organism changes into organic acid instead of GHG carbon dioxide and methane by short chain fatty acid SCFAs (as acetic acid, propionic acid, butyric acid and valeric acid etc.) under anaerobion effect, has important meaning to carbon emission reduction.
(2), short chain fatty acid SCFAs can supplement the deficiency of microorganism carbon source when degradation of organic substances, realizes the dephosphorization denitrogenation to the low sewage of river COD concentration; And short chain fatty acid SCFAs is when biological degradation mud is organic, can be solvable COD by about 80% organic matter transformation.
(3) hazardous and noxious substances of a large amount of pathogenic agent, contained in short chain fatty acid SCFAs degradable mud, parasite, heavy metal element and some difficult degradations, carry out harmless treatment again, harmless treatment cost can being reduced, avoiding river to gush mud for bringing secondary pollution to other soil and water surrounding during other purposes.
(4), adopt short chain fatty acid SCFAs to gush mud to river to degrade, the cost of Sludge landfill place and trucking costs can be reduced.
(5), short chain fatty acid SCFAs only need seal anaerobism, storage, convenient transportation.
(6), the mineralized water quality scavenging agent of short chain fatty acid SCFAs and load magnetotaxis plant-sourced microorganism is after microcapsulary is integrated, in use, only directly need spread fertilizer over the fields and throw in river and gush, river will be sunk to rapidly and gush end mud and do not run off, easy and simple to handle; The mineralized water quality scavenging agent of combining short chain fatty acid SCFAs and load magnetotaxis plant-sourced microorganism again gushes sludge hydrolytic to river and produces the impact of acid, the soluble protein produced in discovery sludge hydrolytic process and carbohydrate change with fermentation time substantially, the efficiency of excess sludge hydrolysis wants high, can make volatile suspended solid in mud (VSS) decrement, its minimizing degree is 30-40%.
(7), the microcapsules of the mineralized water quality scavenging agent of short chain fatty acid SCFAs and load magnetotaxis plant-sourced microorganism facilitates degraded and the acidization of the rear small organic molecule (amino acid and monose) of the dissolving of particulate organic matter in the usual mud in river, the hydrolysis of solubilised state larger molecular organics (protein and carbohydrate) and hydrolysis, and reduce the activity of methanogen, thus make the intermediate product short chain fatty acid SCFAs of excess sludge anaerobic fermentation process be able to a large amount of accumulation, suppress river to gush foul smell and produce.
Below in conjunction with specific embodiment, the present invention is described in detail.
The preparation method of a kind of sewage deodorizing degraded mud short chain fatty acid provided by the invention, comprises the following steps:
The first step, preparation vegetable-protein liquid;
The vegetable-proteins such as the cereal beans of 30-40 part are added in the pure water of 100 parts and grind pulping, and sterilize under 90-120 degree high temperature, then it is for subsequent use to cool the temperature to 35-40 degree.
Second step, utilize plant-sourced microorganism to carry out anaerobically fermenting to vegetable-protein liquid, obtain short chain fatty acid SCFAs;
Utilize plant-sourced nutrient solution that animal source fermentable is domesticated for plant-sourced microorganism, now plant-sourced microorganism is all anaerobion;
1 part of plant-sourced microorganism is added in 100 parts of vegetable-protein liquid, uniform stirring, at 35-40 degree temperature lower seal fermentation 24-72 hour, utilize the hydrolysis of vegetable-protein and acidifying to obtain short chain fatty acid SCFAs.
The present invention utilizes plant-sourced microorganism not only to ferment to the method that cereal beans vegetable-protein carries out anaerobically fermenting can steady temperature, pH, redox potential and fermentation time; And can ectocine be avoided, obtain high-quality, highly purified short chain fatty acid SCFAs when ensureing low cost.
3rd step, microcapsulary is utilized to obtain short chain fatty acid SCFAs microcapsules, to adapt to sewage sludge environment purification;
Be 1%-5% sodium alginate soln by concentration be that 5% calcium chloride solution mixes according to the ratio of 1:1 with concentration, again the mineralized water quality scavenging agent of 1 part of mixing solutions, 1 part of load magnetotaxis plant-sourced microorganism and 100 parts of short chain fatty acid SCFAs obtained are mixed, uniform stirring, obtains short chain fatty acid SCFAs microcapsules.
In the present invention, the preparation method of the mineralized water quality scavenging agent of load magnetotaxis plant-sourced microorganism is:
Utilize plant-sourced nutrient solution that animal source fermentable is domesticated for plant-sourced microorganism, wherein, animal source microorganism comprises the anaerobion and aerobic microbiological that ratio of weight and number is 1:1;
Plant-sourced fermentable is domesticated for plant-sourced magnetotaxis microorganism, then is carried on sand-like mineral stone flour, generate microorganism purification of water quality microbial inoculum.
By the short chain fatty acid SCFAs microcapsules of above-mentioned acquisition according to a certain percentage (every square metre of 50-150 gram) be spread on river and gush, the organism of mud is gushed in degraded river, reach the effect of denitrogenation phosphorus decomposing deodorizing, because this short chain fatty acid SCFAs is microcapsules, thus avoid river and gush Heavy Metals in Sludge and other objectionable impurities affects short chain fatty acid SCFAs, improve river and gush purification efficiency, promote that self-cleaning ability is gushed in river; This kind of short chain fatty acid SCFAs microcapsule inclusion also can be used for atmospheric pollution process deodorizing and refuse landfill simultaneously, and principle is consistent with degraded mud.
In the present invention, in second step, utilize plant-sourced nutrient solution that animal source fermentable is domesticated for plant-sourced microorganism, concrete steps are as follows:
In the pure water of 100 parts, add the vegetable-derived media of 30-40 part, then add the anaerobion of 3-10 part, stir, constant temperature 35 DEG C of sealing and fermenting 24-48 hours;
Add the vegetable-derived media of 30-40 part and the aerobic microbiological of 3-10 part again, stir, constant temperature 30 DEG C fermentation 12-24 hour, every 15-30 minute oxygenic aeration once; Then, constant temperature 25 DEG C fermentation 8-12 hour, every 30-60 minute oxygenic aeration once; Last spontaneous fermentation 12-24 hour, every 120--240 minute oxygenic aeration once.
In the present invention, according to microbial physiology metabolic function, the microorganism being domesticated for plant-sourced by animal source includes but not limited to:
A, protein decomposing bacteria clostridium butylicum, the effect of this bacterioid is that protein hydrolysate forms amino acid, then resolves into organic acid, mercaptan, ammonia and hydrogen sulfide further.
B, carbohydrate breakdown bacterium yeast, the effect of this bacterioid is that hydrolyze carbohydrates becomes glucose, preponderates to have endosporic rod bacterium; Such as acetone, butanols clostridium, they can decompose carbohydrate and produce acetone, butanols, acetic acid and hydrogen etc., and these clostridium are anaerobism, sporiferous bacterium, and therefore they can survive under rugged environment condition.
C, steatolysis bacterium streptococcus faecium, comprise milk-acid bacteria, plant lactobacillus and Lactobacterium acidophilum, and the function of this bacterioid is that steatolysis is become lipid acid, and lipid acid resolves into short chain fatty acid SCFAs and carbonic acid gas more further.
D, cellulose-decomposing bacterium EM bacterium, this bacterioid participates in cellulosic decomposition, and cellulosic decomposition is the essential step of anaerobic digestion, plays a part restriction to digestion rate.This bacterioid utilizes Mierocrystalline cellulose and is translated into CO
2, H
2, ethanol and acetic acid.
E, hydrogen-producing acetogens and homoacetogenic bacteria, can degrade in digester aromatic acid and other organic acid of hydrogen-producing acetogens generates acetic acid and H
2, also form CO when degrading odd number carbon element
2; Pyruvic acid is the main intermediate product of microbiological deterioration carbohydrate, and conversion of pyruvate under anaerobic can be become acetic acid and CO by some hydrogenogenss
2, and release H
2; Homoacetogenic bacteria can by one-carbon compound (as H
2/ CO
2or formic acid etc.) or the metabolism of many carbon compounds be acetic acid.
Visible, in mentioned microorganism, clostridium butylicum, yeast, milk-acid bacteria, plant lactobacillus, Lactobacterium acidophilum, EM bacterium have been the microorganisms of acidication effect, can be referred to as hydrolysis anaerobically fermenting bacterium; Hydrogen-producing acetogens and homoacetogenic bacteria are the bacterium of special decomposition or synthesis of acetic acid, they can be referred to as fermentation and acid bacterium together here, and above two kinds of bacteriums are all obligatory anaerobic bacteria.
In theory, the hydrolysis of anaerobically fermenting and souring stage can be distinguished, and in the mixing microorganisms system of reality, except employing lytic enzyme technique, even if strict control condition, hydrolysis and souring stage also cannot be well-separated, this is mainly because hydrolysis bacterium is a kind of fermenting bacteria with hydrolysis ability, hydrolysis is power consumption process, it is exactly to obtain the water-soluble base carrying out fermenting that fermenting bacteria pays the object that energy is hydrolyzed, and obtained by the biochemical reaction in born of the same parents, discharge meta-bolites short chain fatty acid SCFAs simultaneously.
Obviously, those skilled in the art can carry out various change and modification to the present invention and not depart from the spirit and scope of the present invention.Like this, if these amendments of the present invention and modification belong within the scope of the claims in the present invention and equivalent technologies thereof, then the present invention is also intended to comprise these change and modification.
Claims (3)
1. a preparation for sewage deodorizing degraded mud short chain fatty acid, is characterized in that, comprise the following steps:
The cereal beans vegetable-protein of 30-40 part is added in the pure water of 100 parts and grinds pulping, and sterilize under 90-120 degree high temperature, then cool the temperature to 35-40 degree;
Utilize plant-sourced nutrient solution that animal source fermentable is domesticated for plant-sourced microorganism, 1 part of plant-sourced microorganism is added in 100 parts of vegetable-protein liquid, uniform stirring, at 35-40 degree temperature lower seal fermentation 24-72 hour, obtain short chain fatty acid;
Be 1%-5% sodium alginate soln by concentration be that 5% calcium chloride solution mixes according to ratio of quality and the number of copies 1:1 with concentration, again above-mentioned mixing solutions, the mineralized water quality scavenging agent of load magnetotaxis plant-sourced microorganism and the short chain fatty acid of acquisition are mixed according to ratio of quality and the number of copies 1:1:100, uniform stirring, obtains short chain fatty acid microcapsules.
2. the method for claim 1, is characterized in that, described plant-sourced microorganism is anaerobion.
3. the method for claim 1, is characterized in that, according to microbial physiology metabolic function, the microorganism being domesticated for plant-sourced by animal source includes but not limited to:
Protein decomposing bacteria: clostridium butylicum;
Carbohydrate breakdown bacterium: yeast;
Steatolysis bacterium: streptococcus faecium, milk-acid bacteria, plant lactobacillus and Lactobacterium acidophilum;
Cellulose-decomposing bacterium: EM bacterium;
Hydrogen-producing acetogens and homoacetogenic bacteria.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108097032A (en) * | 2017-12-29 | 2018-06-01 | 广州广牧丰生物技术有限公司 | A kind of farm's deodorant and the method that poultry production performance is improved with the deodorant |
| CN110643645A (en) * | 2019-10-31 | 2020-01-03 | 中国农业科学院农业环境与可持续发展研究所 | Method for producing medium-chain fatty acid by taking livestock and poultry manure as raw material through two-stage fermentation |
| CN111282987A (en) * | 2020-02-28 | 2020-06-16 | 中科鼎实环境工程有限公司 | Inhibitor for treating peculiar smell of polluted soil and treatment method thereof |
| CN113278573A (en) * | 2021-07-21 | 2021-08-20 | 同济大学 | Continuous directional high-value biotransformation method for strengthening urban wet garbage open system |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108097032A (en) * | 2017-12-29 | 2018-06-01 | 广州广牧丰生物技术有限公司 | A kind of farm's deodorant and the method that poultry production performance is improved with the deodorant |
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| CN111282987A (en) * | 2020-02-28 | 2020-06-16 | 中科鼎实环境工程有限公司 | Inhibitor for treating peculiar smell of polluted soil and treatment method thereof |
| CN113278573A (en) * | 2021-07-21 | 2021-08-20 | 同济大学 | Continuous directional high-value biotransformation method for strengthening urban wet garbage open system |
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Application publication date: 20151223 |