CN104498541A - Method for producing short-chain volatile fatty acids by utilizing kitchen wastes and short-chain volatile fatty acids - Google Patents
Method for producing short-chain volatile fatty acids by utilizing kitchen wastes and short-chain volatile fatty acids Download PDFInfo
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- CN104498541A CN104498541A CN201410841735.7A CN201410841735A CN104498541A CN 104498541 A CN104498541 A CN 104498541A CN 201410841735 A CN201410841735 A CN 201410841735A CN 104498541 A CN104498541 A CN 104498541A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/54—Acetic acid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/52—Propionic acid; Butyric acids
Abstract
The invention discloses a method for producing short-chain volatile fatty acids by utilizing kitchen wastes and the short-chain volatile fatty acids. The method specifically comprises the following steps: uniformly mixing and stirring the kitchen wastes serving as a fermentation substrate and sludge serving as an inoculum, thereby obtaining a fermentation matrix; and adding alkyl polyglucosides into the fermentation matrix, and performing anaerobic fermentation under a stirring condition. The reduction, recycling and innocent treatment of the kitchen wastes are realized, and the method has the advantages of low operating cost, high profit, high yield of the short-chain volatile fatty acids and the like.
Description
Technical field
The present invention relates to solid waste disposal technical field, particularly relate to a kind of method utilizing changing food waste to produce short chain volatile lipid acid, also relate to and produce according to preceding method the short chain volatile lipid acid obtained.
Background technology
Changing food waste (being commonly called as swill) is the important component part of domestic waste, and the annual changing food waste output in the whole nation in 2007 is about 9,000 ten thousand tons, and the output of changing food waste presents the trend obviously risen along with the development of society.The features such as it is high that changing food waste has water ratio, and nutritive substance is abundant, These characteristics causes changing food waste very easily rotten, grows pathogenic bacteria.Can urban environment be had a strong impact on if deal with improperly, even threaten the health of the mankind.Therefore one of the process handling problems key issue becoming field of environment protection of changing food waste.
On the other hand, body eutrophication problem becomes increasingly conspicuous, and Environmental Protection in China technological standard requires increasingly stringent to the nitrogen phosphorus of sewage disposal plant effluent.The realization of biological carbon and phosphorous removal relies on a large amount of degradable organic carbon source in sewage.But the concentration of organic carbon source of in most cases intaking is often not enough.Current Sewage Plant often adopts the mode adding carbon source (such as: sodium acetate, methyl alcohol etc.) to solve the problem of carbon source deficiency in sewage.The good denitrification dephosphorization effect of these organic carbon sources, is widely used, but there is the problem of high cost too.Therefore, exploitation is badly in need of cheaper, effective organic carbon source.
Anaerobic fermentation of kitchen waste produces voltaile fatty acid, while realizing organic matter degradation, can reach the object of resource reclaim recycling.Containing end tunnings such as a large amount of short chain volatile lipid acid (SCFAs) in the fermented liquid of this explained hereafter, these organic carbon sources can as the additional carbon of bio-denitrifying sewage dephosphorization.There is fermentation time long (20 ~ 30 days) in traditional anaerobically fermenting, the defects such as product yield is low; Therefore, be badly in need of a kind of shortening fermentation time and improve product yield treatment process.
Tensio-active agent has the performance of amphiphilic, i.e. wetting ability and lipophilicity, but also has the effect of solubilising, and tensio-active agent therefore can be utilized can to improve the hydrolytic action of changing food waste, thus improves the accumulation volume of short chain fatty acid and shorten fermentation time.Mostly be chemical surfactant (such as, sodium laurylsulfonate, sodium lauryl sulphate etc.) at the tensio-active agent of reinforcement sludge hydrolysis in the past, it is high to there is toxicity in chemical surfactant, and biological degradability is low easily causes the defects such as secondary pollution to environment.And bio-surfactant has the various surface propertys of chemical surfactant, environmentally friendly, utilize bio-surfactant at present
Improve changing food waste anaerobic hydrolysis speed thus carry high acid quantifier elimination and rarely have report.
Summary of the invention
The technical problem to be solved in the present invention overcomes the deficiencies in the prior art, a kind of changing food waste that utilizes is the object of the present invention is to provide to produce the method for short chain volatile lipid acid as substrate, realize the minimizing of changing food waste, resource utilization and harmless treatment, there is running cost cheap, income is high, the advantages such as short chain volatile lipid acid productive rate is high, present invention also offers a kind of preceding method and produce the short chain volatile lipid acid obtained, mainly comprise, acetic acid, propionic acid, butyric acid and valeric acid etc., these short chain volatile lipid acid can as the additional carbon of biological carbon and phosphorous removal, improve the efficiency of denitrogenation dephosphorizing.
For solving the problems of the technologies described above, providing a kind of method utilizing changing food waste to produce short chain volatile lipid acid, comprising the following steps:
S1, being fermentation substrate with changing food waste, is that inoculum carries out stirring and evenly mixing and obtains fermented substrate with mud;
S2, in fermented substrate, add alkyl polyglycoside, under agitation condition, carry out anaerobically fermenting.
Aforesaid method, preferably, also comprises changing food waste pre-treatment step:
(1) by after kitchen garbage pulverizing, carry out dehydration separation and obtain dehydration siccative and oil-water mixture;
(2) oil-water mixture is carried out deoiling treatment and obtain de-oiling sewage;
(3) mixed dehydration siccative and de-oiling sewage.
Aforesaid method, preferably, mud is the excess sludge of second pond in sewage work; Microorganism in excess sludge is one or more in fusobacterium microorganism, Bacteroides microorganism, Butyrivibrio microorganism and bifidus bacillus microorganism belonging to genus.
Aforesaid method, preferably, is undertaken excess sludge after anaerobically fermenting cultivates one month, being re-used as the inoculum in S1 step.
Aforesaid method, preferably, the addition of alkyl polyglycoside is 5w/w% ~ 40 w/w% of solid total mass in fermented substrate.
Aforesaid method, preferably, the volume ratio of fermentation substrate and inoculum is 9: 1.
Aforesaid method, preferably, in S2 step, the temperature of anaerobically fermenting is 35 DEG C ~ 45 DEG C, fermentation time 4 days ~ 15 days.
Aforesaid method, preferably, in S2 step, the initial pH of anaerobically fermenting is 7.0 ± 0.5.
Aforesaid method, preferably, mixing speed 100 r/min ~ 130 r/min of anaerobically fermenting in S2 step.
As a total technical conceive, the present invention should provide a kind of aforementioned method utilizing changing food waste to produce short chain volatile lipid acid and produce the short chain volatile lipid acid obtained.
Compared with prior art, the invention has the advantages that:
(1) the invention provides a kind of method utilizing changing food waste to produce short chain volatile lipid acid, take changing food waste as fermentation substrate, mud is that inoculum carries out anaerobically fermenting, experiment shows that its Nitrogen/Phosphorus Removal of carbon source utilizing anaerobic ferment process to produce obviously is better than traditional carbon source methyl alcohol, ethanol etc., and the low production cost of this technique.Anerobe in mud, if carbohydrate granular in fermentation substrate and protein transduction are macromolecular polysaccharide and polypeptide by fusobacterium, Bacteroides, Butyrivibrio, genus bifidobacterium, and then be converted into short chain volatile lipid acid (as acetic acid, propionic acid etc.), alkyl polyglycoside promotes the hydrolytic action of anaerobically fermenting and effectively inhibits methane phase process, further increases the output of short chain volatile lipid acid.
Owing to the addition of alkyl polyglycoside bio-surfactant (0.2g/g TS) in the present invention, when being 6 days when fermenting, in fermented liquid, the content of short chain voltaile fatty acid is 37.2 g/L, improves nearly 17 times than the control group 2.1g/L not adding alkyl polyglycoside.
(2) the invention provides a kind of method utilizing changing food waste to produce short chain volatile lipid acid, fermentation substrate is changing food waste, inoculum is excess sludge, raw material sources are extensive, without the need to buying, advantages such as having running cost cheap, income is high, short chain volatile lipid acid productive rate is high, achieves the minimizing of changing food waste, resource utilization and harmless treatment.
(3) changing food waste is carried out pre-treatment by the present invention, avoid high oiliness changing food waste and restraining effect is produced to anaerobic fermentation and acid production, macromolecular particle shape Substance Transformation in fermentation substrate can be made to become small molecules dissolved organic matter, thus improve hydrolysis rate, and then shorten fermentation time and improve the efficiency of producing acid.
(4) the invention provides a kind of method utilizing changing food waste to produce short chain volatile lipid acid, in fermented liquid, with the addition of alkyl polyglycoside.Compared with chemical surfactant, alkyl polyglycoside is the more comprehensive new non-ionic bio-surfactant of a kind of performance, there is high surface, lower toxicity, higher biological degradability, under extreme temperature, pH and salt concn, there is the advantages such as good selectivity and specificity, changing food waste anaerobic hydrolysis speed can be increased substantially, shorten the time of anaerobically fermenting, this improvement to Method of Food Wastes Disposal, optimization have certain directive significance; Alkyl polyglycoside is consumed in anaerobically fermenting simultaneously, can not cause environmental pollution.
(5) the present invention adopts the mode of anaerobically fermenting to produce short chain volatile lipid acid, anaerobic fermentation process is sequence batch (, does not need aeration air blast equal energy source to input, and the output of short chain volatile lipid acid significantly improves simultaneously, there is energy consumption low, the advantage such as good in economic efficiency.
(6) fermented liquid of short chain volatile lipid acid of the present invention can introduce biological carbon and phosphorous removal system, improves denitrogenation dephosphorizing efficiency.Filter residue after centrifugal may be used for compost or obtaining liq fertilizer.
Accompanying drawing explanation
For making the object of the embodiment of the present invention, technical scheme and advantage clearly, below in conjunction with the accompanying drawing in the embodiment of the present invention, clear, complete description is carried out to the technical scheme in the embodiment of the present invention.
Fig. 1 is the schema that in embodiment 1, anaerobic fermentation of kitchen waste produces short chain volatile lipid acid.
Embodiment
Below in conjunction with Figure of description and concrete preferred embodiment, the invention will be further described, but protection domain not thereby limiting the invention.
The material adopted in following examples and instrument are commercially available.It is wherein the excess sludge of second pond in sewage treatment plants as the mud of inoculum, specifically take from the excess sludge of Changsha first sewage work secondary sedimentation discharge, the microbe species of excess sludge mainly comprises the fusobacterium, Bacteroides, Butyrivibrio, genus bifidobacterium etc. of obligate anaerobic; Changing food waste as fermentation substrate takes from the changing food waste of Hunan University the 5th dining room normal working day, and main component is leftovers, leftovers etc.The formation of changing food waste is primarily of carbohydrate and protein.
embodiment 1:
See Fig. 1: utilize changing food waste to produce the method for short chain volatile lipid acid, specifically comprise the following steps:
(1) pre-treatment changing food waste: by wooden chopstick in changing food waste, medicated napkin, glass-ceramic sheet and bone, fishbone etc. are unfavorable for that the material fermented is rejected.Then be crushed to less than 0.3 centimetre with pulverizer, then use water extracter to be separated into dehydration siccative and oil-water mixture two portions the changing food waste after pulverizing.Oil-water mixture is entered water-and-oil separator to carry out oily water separation and obtain de-oiling sewage.Mixed with dehydration siccative by de-oiling sewage, solid content is 15%, VS(volatile solid)/TS(total solids)=95.3%; Complete the pre-treatment of changing food waste.
(2) seed sludge: the excess sludge taking from the secondary sedimentation discharge of Changsha first sewage work, after testing, the microorganism in excess sludge is mainly hydrolysis and fermented type microorganism; Be specially the fusobacterium of obligate anaerobic, Bacteroides, Butyrivibrio, genus bifidobacterium.Excess sludge is carried out Anaerobic culturel one month, the temperature of Anaerobic culturel is 35 ~ 45 DEG C, and pH controls 7.0 ± 0.5, and anaerobism stirring velocity is 100 ~ 130 r/min, (carbon source is the sodium acetate of 300 ± 15mgCOD/L, and nitrogenous source is 30 ± 1mgNH to add a nitrogenous source and carbon source every day
4 +the ammonium chloride of-N/L), sludge age controls at 12 days, and anaerobic culture conditions obtains the seed sludge of water ratio 99%, pH=7.0 ± 0.5 thus.
(3) anaerobically fermenting: using 1.8L through step (1) pretreated changing food waste and 0.2L through step (2) seed sludge as fermented substrate, drop into the reaction vessel (i.e. anaerobically fermenting device) that organic glass is made, then the proportion dropping into total solids (TS) in fermented substrate is the alkyl polyglycoside of 0.05g/g TS, and inflated with nitrogen drives oxygen and carries out anaerobically fermenting.The temperature controlling anaerobically fermenting is 35 ~ 45 DEG C, and initial pH is 7 ± 0.5, and mixing speed is 100 ~ 130 r/min.
When being 6 days when fermenting, the content measuring short chain voltaile fatty acid in fermented liquid is 21.4g COD/L(stoichiometrically number calculating).
embodiment 2:
Utilize changing food waste to produce the method for short chain volatile lipid acid, specifically comprise the following steps:
(1) pre-treatment changing food waste: by wooden chopstick in changing food waste, medicated napkin, glass-ceramic sheet and bone, fishbone etc. are unfavorable for that the material fermented is rejected.Then be crushed to less than 0.3 centimetre with pulverizer, then use water extracter to be separated into dehydration siccative and oil-water mixture two portions the changing food waste after pulverizing.Oil-water mixture is entered water-and-oil separator to carry out oily water separation and obtain de-oiling sewage.Mixed with dehydration siccative by de-oiling sewage, solid content is 15%, VS/TS=95.3%; Complete the pre-treatment of changing food waste.
(2) seed sludge: the excess sludge taking from the secondary sedimentation discharge of Changsha first sewage work, after testing, the microorganism in excess sludge is mainly hydrolysis and fermented type microorganism; Be specially the fusobacterium of obligate anaerobic, Bacteroides, Butyrivibrio, genus bifidobacterium etc.Excess sludge is carried out Anaerobic culturel one month, the temperature of Anaerobic culturel is 35 ~ 45 DEG C, and pH controls 7.0 ± 0.5, and anaerobism stirring velocity is 100 ~ 130 r/min, (carbon source is the sodium acetate of 300 ± 15mgCOD/L, and nitrogenous source is 30 ± 1mgNH to add a nitrogenous source and carbon source every day
4 +the ammonium chloride of-N/L), sludge age controls at 12 days, and anaerobic culture conditions obtains the seed sludge of water ratio 99%, pH=7.0 ± 0.5 thus.
(3) anaerobically fermenting: using 1.8L through step (1) pretreated changing food waste and 0.2L through step (2) seed sludge as fermented substrate, drop into the reaction vessel that organic glass is made, then the proportion dropping into total solids (TS) in fermented substrate is the alkyl polyglycoside of 0.1g/g TS, and inflated with nitrogen drives oxygen and carries out anaerobically fermenting.The temperature controlling anaerobically fermenting is 35 ~ 45 DEG C, and initial pH is 7 ± 0.5, and mixing speed is 100 ~ 130 r/min.
When being 6 days when fermenting, the content measuring short chain voltaile fatty acid in fermented liquid is 30.5 g COD/L(stoichiometrically number calculating).
embodiment 3:
(1) pre-treatment changing food waste: by wooden chopstick in changing food waste, medicated napkin, glass-ceramic sheet and bone, fishbone etc. are unfavorable for that the material fermented is rejected.Then be crushed to less than 0.3 centimetre with pulverizer, then use water extracter to be separated into dehydration siccative and oil-water mixture two portions the changing food waste after pulverizing.Oil-water mixture is entered water-and-oil separator to carry out oily water separation and obtain de-oiling sewage.Mixed with dehydration siccative by de-oiling sewage, solid content is 15%, VS/TS=95.3%; Complete the pre-treatment of changing food waste.
(2) seed sludge: the excess sludge taking from the secondary sedimentation discharge of Changsha first sewage work, after testing, the microorganism in excess sludge is mainly hydrolysis and fermented type microorganism; Be specially the fusobacterium of obligate anaerobic, Bacteroides, Butyrivibrio, genus bifidobacterium etc.Excess sludge is carried out Anaerobic culturel one month, the temperature of Anaerobic culturel is 35 ~ 45 DEG C, and pH controls 7.0 ± 0.5, and anaerobism stirring velocity is 100 ~ 130 r/min, (carbon source is the sodium acetate of 300 ± 15mgCOD/L, and nitrogenous source is 30 ± 1mgNH to add a nitrogenous source and carbon source every day
4 +the ammonium chloride of-N/L), sludge age controls at 12 days, and anaerobic culture conditions obtains the seed sludge of water ratio 99%, pH=7.0 ± 0.5 thus.
(3) anaerobically fermenting: using 1.8L through step (1) pretreated changing food waste and 0.2L through step (2) seed sludge as fermented substrate, drop into the reaction vessel that organic glass is made, then the proportion dropping into total solids (TS) in fermented substrate is the alkyl polyglycoside of 0.2g/g TS, and inflated with nitrogen drives oxygen and carries out anaerobically fermenting.The temperature controlling anaerobically fermenting is 35 ~ 45 DEG C, and initial pH is 7 ± 0.5, and mixing speed is 100 ~ 130 r/min.
When being 6 days when fermenting, the content measuring short chain voltaile fatty acid in fermented liquid is 37.2 g COD/L(stoichiometrically number calculating).
embodiment 4:
(1) pre-treatment changing food waste: by wooden chopstick in changing food waste, medicated napkin, glass-ceramic sheet and bone, fishbone etc. are unfavorable for that the material fermented is rejected.Then be crushed to less than 0.3 centimetre with pulverizer, then use water extracter to be separated into dehydration siccative and oil-water mixture two portions the changing food waste after pulverizing.Oil-water mixture is entered water-and-oil separator to carry out oily water separation and obtain de-oiling sewage.Mixed with dehydration siccative by de-oiling sewage, solid content is 15%, VS/TS=95.3%; Complete the pre-treatment of changing food waste.
(2) seed sludge: the excess sludge taking from the secondary sedimentation discharge of Changsha first sewage work, after testing, the microorganism in excess sludge is mainly hydrolysis and fermented type microorganism; Be specially the fusobacterium of obligate anaerobic, Bacteroides, Butyrivibrio, genus bifidobacterium etc.Excess sludge is carried out Anaerobic culturel one month, the temperature of Anaerobic culturel is 35 ~ 45 DEG C, and pH controls 7.0 ± 0.5, and anaerobism stirring velocity is 100 ~ 130 r/min, (carbon source is the sodium acetate of 300 ± 15mgCOD/L, and nitrogenous source is 30 ± 1mgNH to add a nitrogenous source and carbon source every day
4 +the ammonium chloride of-N/L), sludge age controls at 12 days, and anaerobic culture conditions obtains the seed sludge of water ratio 99%, pH=7.0 ± 0.5 thus.
(3) anaerobically fermenting: using 1.8L through step (1) pretreated changing food waste and 0.2L through step (2) seed sludge as fermented substrate, drop into the reaction vessel that organic glass is made, then the proportion dropping into total solids (TS) in fermented substrate is the alkyl polyglycoside of 0.3g/g TS, and inflated with nitrogen drives oxygen and carries out anaerobically fermenting.The temperature controlling anaerobically fermenting is 35 ~ 45 DEG C, and initial pH is 7 ± 0.5, and mixing speed is 100 ~ 130 r/min.
When being 6 days when fermenting, the content measuring short chain voltaile fatty acid in fermented liquid is 38.5 g COD/L(stoichiometrically number calculating).
embodiment 5:
(1) pre-treatment changing food waste: by wooden chopstick in changing food waste, medicated napkin, glass-ceramic sheet and bone, fishbone etc. are unfavorable for that the material fermented is rejected.Then be crushed to less than 0.3 centimetre with pulverizer, then use water extracter to be separated into dehydration siccative and oil-water mixture two portions the changing food waste after pulverizing.Oil-water mixture is entered water-and-oil separator to carry out oily water separation and obtain de-oiling sewage.Mixed with dehydration siccative by de-oiling sewage, solid content is 15%, VS/TS=95.3%; Complete the pre-treatment of changing food waste.
(2) seed sludge: the excess sludge taking from the secondary sedimentation discharge of Changsha first sewage work, after testing, the microorganism in excess sludge is mainly hydrolysis and fermented type microorganism; Be specially the fusobacterium of obligate anaerobic, Bacteroides, Butyrivibrio, genus bifidobacterium etc.Excess sludge is carried out Anaerobic culturel one month, the temperature of Anaerobic culturel is 35 ~ 45 DEG C, and pH controls 7.0 ± 0.5, and anaerobism stirring velocity is 100 ~ 130 r/min, (carbon source is the sodium acetate of 300 ± 15mgCOD/L, and nitrogenous source is 30 ± 1mgNH to add a nitrogenous source and carbon source every day
4 +the ammonium chloride of-N/L), sludge age controls at 12 days, and anaerobic culture conditions obtains the seed sludge of water ratio 99%, pH=7.0 ± 0.5 thus.
(3) anaerobically fermenting: using 1.8L through step (1) pretreated changing food waste and 0.2L through step (2) seed sludge as fermented substrate, drop into the reaction vessel that organic glass is made, then the proportion dropping into total solids (TS) in fermented substrate is the alkyl polyglycoside of 0.4g/g TS, and inflated with nitrogen drives oxygen and carries out anaerobically fermenting.The temperature controlling anaerobically fermenting is 35 ~ 45 DEG C, and initial pH is 7 ± 0.5, and mixing speed is 100 ~ 130 r/min.
When being 6 days when fermenting, the content measuring short chain voltaile fatty acid in fermented liquid is 40.5 g COD/L(stoichiometrically number calculating).
comparative example 1:
(1) pre-treatment changing food waste: by wooden chopstick in changing food waste, medicated napkin, glass-ceramic sheet and bone, fishbone etc. are unfavorable for that the material fermented is rejected.Then be crushed to less than 0.3 centimetre with pulverizer, then use water extracter to be separated into dehydration siccative and oil-water mixture two portions the changing food waste after pulverizing.Oil-water mixture is entered water-and-oil separator to carry out oily water separation and obtain de-oiling sewage.Mixed with dehydration siccative by de-oiling sewage, solid content is 15%, VS/TS=95.3%; Complete the pre-treatment of changing food waste.
(2) seed sludge: the excess sludge taking from the secondary sedimentation discharge of Changsha first sewage work, after testing, the microorganism in excess sludge is mainly hydrolysis and fermented type microorganism; Be specially the fusobacterium of obligate anaerobic, Bacteroides, Butyrivibrio, genus bifidobacterium etc.Excess sludge is carried out Anaerobic culturel one month, the temperature of Anaerobic culturel is 35 ~ 45 DEG C, and pH controls 7.0 ± 0.5, and anaerobism stirring velocity is 100 ~ 130 r/min, (carbon source is the sodium acetate of 300 ± 15mgCOD/L, and nitrogenous source is 30 ± 1mgNH to add a nitrogenous source and carbon source every day
4 +the ammonium chloride of-N/L), sludge age controls at 12 days, and anaerobic culture conditions obtains the seed sludge of water ratio 99%, pH=7.0 ± 0.5 thus.
(3) anaerobically fermenting: using 1.8L through step (1) pretreated changing food waste and 0.2L through step (2) seed sludge as fermented substrate, drop into the reaction vessel that organic glass is made, inflated with nitrogen drives oxygen and carries out anaerobically fermenting.The temperature controlling anaerobically fermenting is 37 DEG C, and initial pH is 7 ± 0.5, and mixing speed is 100 ~ 130 r/min.
When being 6 days when fermenting, the content measuring short chain voltaile fatty acid in fermented liquid is 8.4g COD/L(stoichiometrically number calculating).
comparative example 2:
(1) pre-treatment changing food waste: by wooden chopstick in changing food waste, medicated napkin, glass-ceramic sheet and bone, fishbone etc. are unfavorable for that the material fermented is rejected.Then be crushed to less than 0.3 centimetre with pulverizer, then use water extracter to be separated into dehydration siccative and oil-water mixture two portions the changing food waste after pulverizing.Oil-water mixture is entered water-and-oil separator to carry out oily water separation and obtain de-oiling sewage.Mixed with dehydration siccative by de-oiling sewage, solid content is 15%, VS/TS=95.3%; Complete the pre-treatment of changing food waste.
(2) seed sludge: the excess sludge taking from the secondary sedimentation discharge of Changsha first sewage work, after testing, the microorganism in excess sludge is mainly hydrolysis and fermented type microorganism; Be specially the fusobacterium of obligate anaerobic, Bacteroides, Butyrivibrio, genus bifidobacterium etc.Excess sludge is carried out Anaerobic culturel one month, the temperature of Anaerobic culturel is 35 ~ 45 DEG C, and pH controls 7.0 ± 0.5, and anaerobism stirring velocity is 100 ~ 130 r/min, (carbon source is the sodium acetate of 300 ± 15mgCOD/L, and nitrogenous source is 30 ± 1mgNH to add a nitrogenous source and carbon source every day
4 +the ammonium chloride of-N/L), sludge age controls at 12 days, and anaerobic culture conditions obtains the seed sludge of water ratio 99%, pH=7.0 ± 0.5 thus.
(3) anaerobically fermenting: using 1.8 L through step (1) pretreated changing food waste and 0.2L through step (2) seed sludge as fermented substrate, drop into the reaction vessel that organic glass is made, inflated with nitrogen drives oxygen and carries out anaerobically fermenting.The temperature controlling anaerobically fermenting is 37 DEG C, and initial pH is 7 ± 0.5, and mixing speed is 100 ~ 130 r/min.
When being 14 days when fermenting, the content measuring short chain voltaile fatty acid in fermented liquid is 12.0g COD/L(stoichiometrically number calculating).
From embodiment 1 to embodiment 5, in the fermentation system of changing food waste, add bio-surfactant alkyl polyglycoside, the organism in changing food waste has well been utilized, significantly improves the output of short chain volatile lipid acid.
comparative example 3:
(1) pre-treatment changing food waste: by wooden chopstick in changing food waste, medicated napkin, glass-ceramic sheet and bone, fishbone etc. are unfavorable for that the material fermented is rejected.Then be crushed to less than 0.3 centimetre with pulverizer, then use water extracter to be separated into dehydration siccative and oil-water mixture two portions the changing food waste after pulverizing.Oil-water mixture is entered water-and-oil separator to carry out oily water separation and obtain de-oiling sewage.Mixed with dehydration siccative by de-oiling sewage, solid content is 15%, VS/TS=95.3%; Complete the pre-treatment of changing food waste.
(2) seed sludge: the excess sludge taking from the secondary sedimentation discharge of Changsha first sewage work, after testing, the microorganism in excess sludge is mainly hydrolysis and fermented type microorganism; Be specially the fusobacterium of obligate anaerobic, Bacteroides, Butyrivibrio, genus bifidobacterium etc.Excess sludge is carried out Anaerobic culturel one month, the temperature of Anaerobic culturel is 35 ~ 45 DEG C, and pH controls 7.0 ± 0.5, and anaerobism stirring velocity is 100 ~ 130 r/min, (carbon source is the sodium acetate of 300 ± 15mgCOD/L, and nitrogenous source is 30 ± 1mgNH to add a nitrogenous source and carbon source every day
4 +the ammonium chloride of-N/L), sludge age controls at 12 days, and anaerobic culture conditions obtains the seed sludge of water ratio 99%, pH=7.0 ± 0.5 thus.
(3) anaerobically fermenting: using 1.8L through step (1) pretreated changing food waste and 0.2L through step (2) seed sludge as fermented substrate, drop into the reaction vessel that organic glass is made, then the proportion dropping into total solids (TS) in fermented substrate is the rhamnolipid (bio-surfactant) of 0.2g/g TS, and inflated with nitrogen drives oxygen and carries out anaerobically fermenting.The temperature controlling anaerobically fermenting is 35 ~ 45 DEG C, and initial pH is 7 ± 0.5, and mixing speed is 100 ~ 130 r/min.
When being 6 days when fermenting, the content measuring short chain voltaile fatty acid in fermented liquid is 21.3 g COD/L(stoichiometrically number calculating).
comparative example 4:
(1) pre-treatment changing food waste: by wooden chopstick in changing food waste, medicated napkin, glass-ceramic sheet and bone, fishbone etc. are unfavorable for that the material fermented is rejected.Then be crushed to less than 0.3 centimetre with pulverizer, then use water extracter to be separated into dehydration siccative and oil-water mixture two portions the changing food waste after pulverizing.Oil-water mixture is entered water-and-oil separator to carry out oily water separation and obtain de-oiling sewage.Mixed with dehydration siccative by de-oiling sewage, solid content is 15%, VS/TS=95.3%; Complete the pre-treatment of changing food waste.
(2) seed sludge: the excess sludge taking from the secondary sedimentation discharge of Changsha first sewage work, after testing, the microorganism in excess sludge is mainly hydrolysis and fermented type microorganism; Be specially the fusobacterium of obligate anaerobic, Bacteroides, Butyrivibrio, genus bifidobacterium etc.Excess sludge is carried out Anaerobic culturel one month, the temperature of Anaerobic culturel is 35 ~ 45 DEG C, and pH controls 7.0 ± 0.5, and anaerobism stirring velocity is 100 ~ 130 r/min, (carbon source is the sodium acetate of 300 ± 15mgCOD/L, and nitrogenous source is 30 ± 1mgNH to add a nitrogenous source and carbon source every day
4 +the ammonium chloride of-N/L), sludge age controls at 12 days, and anaerobic culture conditions obtains the seed sludge of water ratio 99%, pH=7.0 ± 0.5 thus.
(3) anaerobically fermenting: using 1.8 L through step (1) pretreated changing food waste and 0.2L through step (2) seed sludge as fermented substrate, drop into the reaction vessel that organic glass is made, then the proportion dropping into total solids (TS) in fermented substrate is the saponin (bio-surfactant) of 0.2g/g TS, and inflated with nitrogen drives oxygen and carries out anaerobically fermenting.The temperature controlling anaerobically fermenting is 35 ~ 45 DEG C, and initial pH is 7 ± 0.5, and mixing speed is 100 ~ 130 r/min.
When being 6 days when fermenting, the content measuring short chain voltaile fatty acid in fermented liquid is 19.6 g COD/L(stoichiometrically number calculating).
comparative example 5:
(1) pre-treatment changing food waste: by wooden chopstick in changing food waste, medicated napkin, glass-ceramic sheet and bone, fishbone etc. are unfavorable for that the material fermented is rejected.Then be crushed to less than 0.3 centimetre with pulverizer, then use water extracter to be separated into dehydration siccative and oil-water mixture two portions the changing food waste after pulverizing.Oil-water mixture is entered water-and-oil separator to carry out oily water separation and obtain de-oiling sewage.Mixed with dehydration siccative by de-oiling sewage, solid content is 15%, VS/TS=95.3%; Complete the pre-treatment of changing food waste.
(2) seed sludge: the excess sludge taking from the secondary sedimentation discharge of Changsha first sewage work, after testing, the microorganism in excess sludge is mainly hydrolysis and fermented type microorganism; Be specially the fusobacterium of obligate anaerobic, Bacteroides, Butyrivibrio, genus bifidobacterium etc.Excess sludge is carried out Anaerobic culturel one month, the temperature of Anaerobic culturel is 35 ~ 45 DEG C, and pH controls 7.0 ± 0.5, and anaerobism stirring velocity is 100 ~ 130r/min, (carbon source is the sodium acetate of 300 ± 15mgCOD/L, and nitrogenous source is 30 ± 1mgNH to add a nitrogenous source and carbon source every day
4 +the ammonium chloride of-N/L), sludge age controls at 12 days, and anaerobic culture conditions obtains the seed sludge of water ratio 99%, pH=7.0 ± 0.5 thus.
(3) amphimicrobian fermentation: using 1.8 L through step (1) pretreated changing food waste and 0.2L through step (2) seed sludge as fermented substrate, drop into the reaction vessel that organic glass is made, then the proportion dropping into total solids (TS) in fermented substrate is the alkyl polyglycoside of 0.2g/g TS, carry out amphimicrobian fermentation, controlling air intake in amphimicrobian fermenting process is 0.60 ~ 0.8 m
3/ h, ventilatory cycle is 10 ~ 15 min/h, and temperature is 35 ~ 45 DEG C, and initial pH is 7 ± 0.5, and mixing speed is 100 ~ 130 r/min.
When being 6 days when fermenting, the content measuring short chain voltaile fatty acid in fermented liquid is 12.5 g COD/L(stoichiometrically number calculating).
The above is only preferred embodiment of the present invention, not does any pro forma restriction to the present invention.Although the present invention discloses as above with preferred embodiment, but and be not used to limit the present invention.Any those of ordinary skill in the art, when not departing from spirit of the present invention and technical scheme, the Method and Technology content of above-mentioned announcement all can be utilized to make many possible variations and modification to technical solution of the present invention, or be revised as the Equivalent embodiments of equivalent variations.Therefore, every content not departing from technical solution of the present invention, according to technical spirit of the present invention to any simple modification made for any of the above embodiments, equivalent replacement, equivalence change and modification, all still belongs in the scope of technical solution of the present invention protection.
Claims (10)
1. utilize changing food waste to produce a method for short chain volatile lipid acid, it is characterized in that, comprise the following steps:
S1, being fermentation substrate with changing food waste, is that inoculum carries out stirring and evenly mixing and obtains fermented substrate with mud;
S2, in described fermented substrate, add alkyl polyglycoside, under agitation condition, carry out anaerobically fermenting.
2. method according to claim 1, is characterized in that, also comprises changing food waste pre-treatment step:
(1) by after described kitchen garbage pulverizing, carry out dehydration separation and obtain dehydration siccative and oil-water mixture;
(2) described oil-water mixture is carried out deoiling treatment and obtain de-oiling sewage;
(3) described dehydration siccative and de-oiling sewage is mixed.
3. method according to claim 1, is characterized in that, described mud is the excess sludge of second pond in sewage work; Microorganism in described excess sludge is one or more in fusobacterium microorganism, Bacteroides microorganism, Butyrivibrio microorganism and bifidus bacillus microorganism belonging to genus.
4. method according to claim 3, is characterized in that, is carried out by described excess sludge after anaerobically fermenting cultivates one month, being re-used as the inoculum in described S1 step.
5. method according to any one of claim 1 to 4, is characterized in that, the addition of described alkyl polyglycoside is 5w/w% ~ 40 w/w% of solid total mass in described fermented substrate.
6. method according to any one of claim 1 to 4, is characterized in that, the volume ratio of described fermentation substrate and described inoculum is 9: 1.
7. method according to any one of claim 1 to 4, is characterized in that, the temperature of anaerobically fermenting described in described S2 step is 35 DEG C ~ 45 DEG C, fermentation time 4 days ~ 15 days.
8. method according to any one of claim 1 to 4, is characterized in that, described in described S2 step, the initial pH of anaerobically fermenting is 7 ± 0.5.
9. method according to any one of claim 1 to 4, is characterized in that, mixing speed 100 r/min ~ 130 r/min of anaerobically fermenting described in described S2 step.
10. the method utilizing changing food waste to produce short chain volatile lipid acid according to any one of a claim 1 to 9 produces the short chain volatile lipid acid obtained.
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| CN106801082A (en) * | 2017-02-09 | 2017-06-06 | 广西中烟工业有限责任公司 | A kind of method that utilization tobacco waste produces short chain volatile aliphatic acid |
| CN107760728A (en) * | 2016-08-18 | 2018-03-06 | 湖南大学 | A kind of method using Dregs Manufacture short chain volatile aliphatic acid |
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| CN106801082A (en) * | 2017-02-09 | 2017-06-06 | 广西中烟工业有限责任公司 | A kind of method that utilization tobacco waste produces short chain volatile aliphatic acid |
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| CN110042128A (en) * | 2019-04-30 | 2019-07-23 | 河海大学 | A method of it improving changing food waste into resources and utilizes |
| CN110862207B (en) * | 2019-11-28 | 2021-10-26 | 湖南大学 | Method for promoting degradation of capsaicin in kitchen waste and simultaneously producing methane |
| CN110862207A (en) * | 2019-11-28 | 2020-03-06 | 湖南大学 | Method for promoting degradation of capsaicin in kitchen waste and simultaneously producing methane |
| CN110951789A (en) * | 2019-12-17 | 2020-04-03 | 清华大学深圳国际研究生院 | Kitchen waste treatment method and system |
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| CN114875076A (en) * | 2022-06-28 | 2022-08-09 | 昆明理工大学 | Technical method for producing ethanol by combined fermentation of sludge and kitchen waste |
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