CN104498541B - The method and short chain volatile aliphatic acid of short chain volatile aliphatic acid are produced using kitchen garbage - Google Patents
The method and short chain volatile aliphatic acid of short chain volatile aliphatic acid are produced using kitchen garbage Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
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- C12P7/54—Acetic acid
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/52—Propionic acid; Butyric acids
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Abstract
The invention discloses a kind of method of utilization kitchen garbage production short chain volatile aliphatic acid and short chain volatile aliphatic acid, its specific method comprises the following steps：Using kitchen garbage as fermentation substrate, it is stirred using sludge as inoculum and is uniformly mixed so as to obtain fermentation substrate；Added into fermentation substrate and anaerobic fermentation is carried out under alkyl polyglycoside, stirring condition.The present invention realizes minimizing, recycling and the harmless treatment of kitchen garbage, cheap with operating cost, the advantage such as income height, short chain volatile aliphatic acid yield height.
Description
Technical field
The present invention relates to Solid Waste Treatment technical field, more particularly to one kind short chain volatile is produced using kitchen garbage
The method of aliphatic acid, further relates to produce obtained short chain volatile aliphatic acid according to preceding method.
Background technology
Kitchen garbage（It is commonly called as swill）It is the important component of domestic waste, the annual kitchen rubbish in the whole nation in 2007
Rubbish yield is about 90,000,000 tons, and the trend that substantially rises is presented with the development of society in the yield of kitchen garbage.Kitchen rubbish
Rubbish has moisture content high, the features such as nutriment enriches, and These characteristics cause kitchen garbage easily to rot, and grows pathogen.If
It is to deal with improperly that urban environment can be had a strong impact on, or even threatens the health of the mankind.Therefore the processing handling problems of kitchen garbage
One of key issue as field of environment protection.
On the other hand, body eutrophication problem becomes increasingly conspicuous, and Environmental Protection in China technical standard is to sewage disposal plant effluent
Nitrogen phosphorus requires increasingly strict.The realization of biological carbon and phosphorous removal relies on a large amount of degradable organic carbon sources in sewage.However, most of
In the case of water inlet organic carbon source concentration it is often not enough.Current sewage plant is frequently with adding carbon source（For example：Sodium acetate, methanol etc.）
Mode solve in sewage the problem of carbon source is not enough.The good denitrification dephosphorization effect of these organic carbon sources, is widely used, but also same
There is the problem of cost is too high in sample.Therefore, it is badly in need of developing cheaper, effective organic carbon source.
Anaerobic fermentation of kitchen waste produces volatile fatty acid, can reach that resource is returned while organic matter degradation is realized
Receive the purpose recycled.Contain substantial amounts of short chain volatile aliphatic acid in the zymotic fluid of the technique productions（SCFAs）Deng end hair
Ferment product, these organic carbon sources can as bio-denitrifying sewage dephosphorization additional carbon.There is fermentation in traditional anaerobic fermentation
Time is long（20~30 days）, the low defect of product yield；Therefore, it is badly in need of a kind of shortening fermentation time and improves product yield
Processing method.
Surfactant has the performance of amphiphilic, i.e. hydrophily and lipophile, but also with the effect of solubilising, therefore can
So that the hydrolysis of kitchen garbage can be improved using surfactant, so as to improve the accumulation of short chain fatty acids and shorten hair
The ferment time.The surfactant hydrolyzed in conventional reinforcement sludge is generally chemical surfactant（For example, dodecyl sodium sulfate,
Lauryl sodium sulfate etc.）, chemical surfactant has that toxicity is high, and biological degradability is low to easily cause secondary pollution etc. to environment
Defect.It is environmentally friendly and biosurfactant has the various surface propertys of chemical surfactant, currently with biology
Surfactant
Kitchen garbage anaerobic hydrolysis speed is improved to be rarely reported so as to carry high acid quantifier elimination.
The content of the invention
The technical problem to be solved in the present invention is to overcome the deficiencies in the prior art, and it is an object of the invention to provide a kind of profit
The method for producing short chain volatile aliphatic acid as substrate with kitchen garbage, realizes minimizing, recycling and the nothing of kitchen garbage
Evilization processing, the advantage such as income height, short chain volatile aliphatic acid yield height cheap with operating cost, present invention also offers
The short chain volatile aliphatic acid that a kind of preceding method production is obtained, mainly includes, acetic acid, propionic acid, butyric acid and valeric acid etc., these are short
Chain volatile fatty acid can as biological carbon and phosphorous removal additional carbon, improve denitrogenation dephosphorizing efficiency.
In order to solve the above technical problems, the method for short chain volatile aliphatic acid is produced there is provided a kind of utilization kitchen garbage,
Comprise the following steps：
S1, using kitchen garbage as fermentation substrate, be stirred using sludge as inoculum and be uniformly mixed so as to obtain fermentation substrate；
S2, added into fermentation substrate anaerobic fermentation is carried out under alkyl polyglycoside, stirring condition.
Foregoing method, it is preferred that also including kitchen garbage pre-treatment step：
（1）After kitchen garbage pulverizing, the isolated dehydration siccative of dehydration and oil water mixture are carried out；
（2）Oil water mixture progress deoiling treatment is obtained into de-oiling sewage；
（3）Mixed dehydration siccative and de-oiling sewage.
Foregoing method, it is preferred that sludge is the excess sludge of second pond in sewage treatment plant；Micro- life in excess sludge
Thing be clostridium microorganism belonging to genus, Bacteroides microorganism, Butyrivibrio microorganism and one kind in Bifidobacterium microorganism belonging to genus or
It is a variety of.
Foregoing method, it is preferred that after excess sludge is carried out into anaerobic fermentation culture one month, be re-used as in S1 steps
Inoculum.
Foregoing method, it is preferred that the addition of alkyl polyglycoside is 5w/w%~40 of solid gross mass in fermentation substrate
w/w%。
Foregoing method, it is preferred that the volume ratio of fermentation substrate and inoculum is 9: 1.
Foregoing method, it is preferred that the temperature of anaerobic fermentation is 35 DEG C~45 DEG C, fermentation time 4 days~15 in S2 steps
My god.
Foregoing method, it is preferred that the initial pH of anaerobic fermentation is 7.0 ± 0.5 in S2 steps.
Foregoing method, it is preferred that the r/min of the r/min of speed of agitator 100 of anaerobic fermentation in S2 steps~130.
As a total technical concept, the present invention should provide a kind of foregoing utilization kitchen garbage production short chain volatile
The short chain volatile aliphatic acid that the method production of aliphatic acid is obtained.
Compared with prior art, the advantage of the invention is that：
（1）The invention provides a kind of method that utilization kitchen garbage produces short chain volatile aliphatic acid, with kitchen garbage
For fermentation substrate, sludge is that inoculum carries out anaerobic fermentation, and experiment shows to utilize its denitrogenation of the carbon source of anaerobic ferment process generation
Phosphor-removing effect is substantially better than traditional carbon source methanol, ethanol etc., and the low production cost of this technique.Anaerobic bacteria in sludge,
If fusobacterium, Bacteroides, Butyrivibrio, Bifidobacterium are by granular carbohydrate and protein in fermentation substrate
Switch to the polysaccharide and polypeptide of macromolecular, and then be converted into short chain volatile aliphatic acid（Such as acetic acid, propionic acid etc.）, alkyl polyglycoside promotion
The hydrolysis of anaerobic fermentation simultaneously effectively inhibits methane phase process, further increases the yield of short chain volatile aliphatic acid.
Due to the addition of alkyl polyglycoside biosurfactant in the present invention（0.2g/g TS）, when fermenting between be 6 days
When, the content of short chain volatile aliphatic acid is 37.2 g/L in zymotic fluid, than the control group 2.1g/L without addition alkyl polyglycoside
Improve nearly 17 times.
（2）The invention provides a kind of method that utilization kitchen garbage produces short chain volatile aliphatic acid, fermentation substrate is
Kitchen garbage, inoculum is excess sludge, and raw material sources are extensively, cheap with operating cost without purchase, and income is high, short chain
The advantages such as volatile fatty acid yield height, realize minimizing, recycling and the harmless treatment of kitchen garbage.
（3）The present invention is pre-processed kitchen garbage, it is to avoid high oiliness kitchen garbage is to anaerobic fermentation and acid production generation
Inhibitory action, can make macromolecular particle shape material in fermentation substrate change into small molecule dissolved organic matter, so as to improve
Hydrolysis rate, and then shorten fermentation time and improve the efficiency of production acid.
（4）The invention provides a kind of method that utilization kitchen garbage produces short chain volatile aliphatic acid, in zymotic fluid
It with the addition of alkyl polyglycoside.Compared with chemical surfactant, alkyl polyglycoside is that a kind of more comprehensive new non-ionic of performance is biological
Surfactant, with high surface, relatively low toxicity, higher biological degradability, under extreme temperature, pH and salinity
There is preferable selectivity and selectivity, kitchen garbage anaerobic hydrolysis speed can be increased substantially, shorten anaerobic fermentation
Time, this improvement to Method of Food Wastes Disposal, optimization have certain directive significance；Alkyl polyglycoside is sent out in anaerobism simultaneously
It is consumed in ferment, does not result in environmental pollution.
（5）The present invention produces short chain volatile aliphatic acid by the way of anaerobic fermentation, and anaerobic fermentation process is batch-type,
The input of air blast equal energy source need not be aerated, while the yield of short chain volatile aliphatic acid is significantly improved, warp low with energy resource consumption
The advantages of helping profitable.
（6）The zymotic fluid of the short chain volatile aliphatic acid of the present invention can introduce biological carbon and phosphorous removal system, improve denitrogenation
Dephosphorization efficiency.Filter residue after centrifugation can be used for compost or prepare liquid fertilizer.
Brief description of the drawings
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below in conjunction with the embodiment of the present invention
In accompanying drawing, clear, complete description is carried out to the technical scheme in the embodiment of the present invention.
Fig. 1 is the flow chart of anaerobic fermentation of kitchen waste production short chain volatile aliphatic acid in embodiment 1.
Embodiment
Below in conjunction with Figure of description and specific preferred embodiment, the invention will be further described, but not therefore and
Limit the scope of the invention.
Material and instrument employed in following examples are commercially available.It is sanitary sewage wherein as the sludge of inoculum
The excess sludge of second pond in treatment plant, is specifically derived from the excess sludge of the first sewage treatment plant of Changsha secondary precipitation discharge, remains
The microbe species of remaining sludge mainly include fusobacterium, Bacteroides, Butyrivibrio, Bifidobacterium of obligate anaerobic etc.；
The kitchen garbage of the dining room regular working day of Hunan University the 5th is derived from as the kitchen garbage of fermentation substrate, main component is surplus
Meal, leftovers etc..The composition of kitchen garbage is main by carbohydrate and protein.
Embodiment 1：
Referring to Fig. 1：The method that short chain volatile aliphatic acid is produced using kitchen garbage, specifically includes following steps：
（1）Pre-process kitchen garbage：By wooden chopstick in kitchen garbage, napkin paper, glass ceramics piece and bone, fishbone etc.
The material for being unfavorable for fermentation is rejected.Then less than 0.3 centimetre is crushed to pulverizer, then by the kitchen garbage after crushing with de-
Water dispenser is separated into dehydration siccative and oil water mixture two parts.Oil water mixture is discharged into oil water separator progress water-oil separating to obtain
To de-oiling sewage.De-oiling sewage is mixed with dehydration siccative, solid content is 15%, VS（Volatile solid）/TS（Total solid）=
95.3%；Complete the pretreatment of kitchen garbage.
（2）Seed sludge：The excess sludge of the first sewage treatment plant of Changsha secondary precipitation discharge is derived from, it is after testing, remaining
Microorganism in sludge is mainly hydrolysis and fermented type microorganism；The specially fusobacterium of obligate anaerobic, Bacteroides, butyric acid arc
Pseudomonas, Bifidobacterium.Excess sludge is subjected to Anaerobic culturel one month, the temperature of Anaerobic culturel is 35~45 DEG C, pH controls
7.0 ± 0.5, anaerobism mixing speed is 100~130 r/min, and a nitrogen source and carbon source are added daily（Carbon source be 300 ±
15mgCOD/L sodium acetate, nitrogen source is 30 ± 1mgNH4 +- N/L ammonium chloride）, sludge age controlled at 12 days, thus Anaerobic culturel
Condition obtains moisture content 99%, the seed sludge of pH=7.0 ± 0.5.
（3）Anaerobic fermentation：Step is passed through with 1.8L（1）The kitchen garbage and 0.2L of pretreatment pass through step（2）Inoculation is dirty
The reaction vessel that mud is made as fermentation substrate, input organic matter glass（That is anaerobic fermentation device）, then put into fermentation substrate
Total solid（TS）Proportion be 0.05g/g TS alkyl polyglycoside, nitrogen charging gas drive oxygen carry out anaerobic fermentation.Control anaerobic fermentation
Temperature is 35~45 DEG C, and initial pH is 7 ± 0.5, and speed of agitator is 100~130 r/min.
When being 6 days between when fermenting, the content for determining short chain volatile aliphatic acid in zymotic fluid is 21.4g COD/L（With
Stoichiometric number is calculated）.
Embodiment 2：
The method that short chain volatile aliphatic acid is produced using kitchen garbage, specifically includes following steps：
（1）Pre-process kitchen garbage：By wooden chopstick in kitchen garbage, napkin paper, glass ceramics piece and bone, fishbone etc.
The material for being unfavorable for fermentation is rejected.Then less than 0.3 centimetre is crushed to pulverizer, then by the kitchen garbage after crushing with de-
Water dispenser is separated into dehydration siccative and oil water mixture two parts.Oil water mixture is discharged into oil water separator progress water-oil separating to obtain
To de-oiling sewage.De-oiling sewage is mixed with dehydration siccative, solid content is 15%, VS/TS=95.3%；Complete the pre- of kitchen garbage
Processing.
（2）Seed sludge：The excess sludge of the first sewage treatment plant of Changsha secondary precipitation discharge is derived from, it is after testing, remaining
Microorganism in sludge is mainly hydrolysis and fermented type microorganism；The specially fusobacterium of obligate anaerobic, Bacteroides, butyric acid arc
Pseudomonas, Bifidobacterium etc..Excess sludge is subjected to Anaerobic culturel one month, the temperature of Anaerobic culturel is 35~45 DEG C, pH controls
System is 7.0 ± 0.5, and anaerobism mixing speed is 100~130 r/min, and a nitrogen source and carbon source are added daily（Carbon source be 300 ±
15mgCOD/L sodium acetate, nitrogen source is 30 ± 1mgNH4 +- N/L ammonium chloride）, sludge age controlled at 12 days, thus Anaerobic culturel
Condition obtains moisture content 99%, the seed sludge of pH=7.0 ± 0.5.
（3）Anaerobic fermentation：Step is passed through with 1.8L（1）The kitchen garbage and 0.2L of pretreatment pass through step（2）Inoculation is dirty
The reaction vessel that mud is made as fermentation substrate, input organic matter glass, then puts into total solid in fermentation substrate（TS）Ratio
Weight is 0.1g/g TS alkyl polyglycoside, and nitrogen charging gas drive oxygen carries out anaerobic fermentation.The temperature for controlling anaerobic fermentation is 35~45 DEG C,
Initial pH is 7 ± 0.5, and speed of agitator is 100~130 r/min.
When being 6 days between when fermenting, the content for determining short chain volatile aliphatic acid in zymotic fluid is 30.5 g COD/L（With
Stoichiometric number is calculated）.
Embodiment 3：
（1）Pre-process kitchen garbage：By wooden chopstick in kitchen garbage, napkin paper, glass ceramics piece and bone, fishbone etc.
The material for being unfavorable for fermentation is rejected.Then less than 0.3 centimetre is crushed to pulverizer, then by the kitchen garbage after crushing with de-
Water dispenser is separated into dehydration siccative and oil water mixture two parts.Oil water mixture is discharged into oil water separator progress water-oil separating to obtain
To de-oiling sewage.De-oiling sewage is mixed with dehydration siccative, solid content is 15%, VS/TS=95.3%；Complete the pre- of kitchen garbage
Processing.
（2）Seed sludge：The excess sludge of the first sewage treatment plant of Changsha secondary precipitation discharge is derived from, it is after testing, remaining
Microorganism in sludge is mainly hydrolysis and fermented type microorganism；The specially fusobacterium of obligate anaerobic, Bacteroides, butyric acid arc
Pseudomonas, Bifidobacterium etc..Excess sludge is subjected to Anaerobic culturel one month, the temperature of Anaerobic culturel is 35~45 DEG C, pH controls
System is 7.0 ± 0.5, and anaerobism mixing speed is 100~130 r/min, and a nitrogen source and carbon source are added daily（Carbon source be 300 ±
15mgCOD/L sodium acetate, nitrogen source is 30 ± 1mgNH4 +- N/L ammonium chloride）, sludge age control was at 12 days, and thus anaerobism is trained
Foster condition obtains moisture content 99%, the seed sludge of pH=7.0 ± 0.5.
（3）Anaerobic fermentation：Step is passed through with 1.8L（1）The kitchen garbage and 0.2L of pretreatment pass through step（2）Inoculation is dirty
The reaction vessel that mud is made as fermentation substrate, input organic matter glass, then puts into total solid in fermentation substrate（TS）Ratio
Weight is 0.2g/g TS alkyl polyglycoside, and nitrogen charging gas drive oxygen carries out anaerobic fermentation.The temperature for controlling anaerobic fermentation is 35~45 DEG C,
Initial pH is 7 ± 0.5, and speed of agitator is 100~130 r/min.
When being 6 days between when fermenting, the content for determining short chain volatile aliphatic acid in zymotic fluid is 37.2 g COD/L（With
Stoichiometric number is calculated）.
Embodiment 4：
（1）Pre-process kitchen garbage：By wooden chopstick in kitchen garbage, napkin paper, glass ceramics piece and bone, fishbone etc.
The material for being unfavorable for fermentation is rejected.Then less than 0.3 centimetre is crushed to pulverizer, then by the kitchen garbage after crushing with de-
Water dispenser is separated into dehydration siccative and oil water mixture two parts.Oil water mixture is discharged into oil water separator progress water-oil separating to obtain
To de-oiling sewage.De-oiling sewage is mixed with dehydration siccative, solid content is 15%, VS/TS=95.3%；Complete the pre- of kitchen garbage
Processing.
（2）Seed sludge：The excess sludge of the first sewage treatment plant of Changsha secondary precipitation discharge is derived from, it is after testing, remaining
Microorganism in sludge is mainly hydrolysis and fermented type microorganism；The specially fusobacterium of obligate anaerobic, Bacteroides, butyric acid arc
Pseudomonas, Bifidobacterium etc..Excess sludge is subjected to Anaerobic culturel one month, the temperature of Anaerobic culturel is 35~45 DEG C, pH controls
System is 7.0 ± 0.5, and anaerobism mixing speed is 100~130 r/min, and a nitrogen source and carbon source are added daily（Carbon source be 300 ±
15mgCOD/L sodium acetate, nitrogen source is 30 ± 1mgNH4 +- N/L ammonium chloride）, sludge age controlled at 12 days, thus Anaerobic culturel
Condition obtains moisture content 99%, the seed sludge of pH=7.0 ± 0.5.
（3）Anaerobic fermentation：Step is passed through with 1.8L（1）The kitchen garbage and 0.2L of pretreatment pass through step（2）Inoculation is dirty
The reaction vessel that mud is made as fermentation substrate, input organic matter glass, then puts into total solid in fermentation substrate（TS）Ratio
Weight is 0.3g/g TS alkyl polyglycoside, and nitrogen charging gas drive oxygen carries out anaerobic fermentation.The temperature for controlling anaerobic fermentation is 35~45 DEG C,
Initial pH is 7 ± 0.5, and speed of agitator is 100~130 r/min.
When being 6 days between when fermenting, the content for determining short chain volatile aliphatic acid in zymotic fluid is 38.5 g COD/L（With
Stoichiometric number is calculated）.
Embodiment 5：
（1）Pre-process kitchen garbage：By wooden chopstick in kitchen garbage, napkin paper, glass ceramics piece and bone, fishbone etc.
The material for being unfavorable for fermentation is rejected.Then less than 0.3 centimetre is crushed to pulverizer, then by the kitchen garbage after crushing with de-
Water dispenser is separated into dehydration siccative and oil water mixture two parts.Oil water mixture is discharged into oil water separator progress water-oil separating to obtain
To de-oiling sewage.De-oiling sewage is mixed with dehydration siccative, solid content is 15%, VS/TS=95.3%；Complete the pre- of kitchen garbage
Processing.
（2）Seed sludge：The excess sludge of the first sewage treatment plant of Changsha secondary precipitation discharge is derived from, it is after testing, remaining
Microorganism in sludge is mainly hydrolysis and fermented type microorganism；The specially fusobacterium of obligate anaerobic, Bacteroides, butyric acid arc
Pseudomonas, Bifidobacterium etc..Excess sludge is subjected to Anaerobic culturel one month, the temperature of Anaerobic culturel is 35~45 DEG C, pH controls
System is 7.0 ± 0.5, and anaerobism mixing speed is 100~130 r/min, and a nitrogen source and carbon source are added daily（Carbon source be 300 ±
15mgCOD/L sodium acetate, nitrogen source is 30 ± 1mgNH4 +- N/L ammonium chloride）, sludge age controlled at 12 days, thus Anaerobic culturel
Condition obtains moisture content 99%, the seed sludge of pH=7.0 ± 0.5.
（3）Anaerobic fermentation：Step is passed through with 1.8L（1）The kitchen garbage and 0.2L of pretreatment pass through step（2）Inoculation is dirty
The reaction vessel that mud is made as fermentation substrate, input organic matter glass, then puts into total solid in fermentation substrate（TS）Ratio
Weight is 0.4g/g TS alkyl polyglycoside, and nitrogen charging gas drive oxygen carries out anaerobic fermentation.The temperature for controlling anaerobic fermentation is 35~45 DEG C,
Initial pH is 7 ± 0.5, and speed of agitator is 100~130 r/min.
When being 6 days between when fermenting, the content for determining short chain volatile aliphatic acid in zymotic fluid is 40.5 g COD/L（With
Stoichiometric number is calculated）.
Comparative example 1：
（1）Pre-process kitchen garbage：By wooden chopstick in kitchen garbage, napkin paper, glass ceramics piece and bone, fishbone etc.
The material for being unfavorable for fermentation is rejected.Then less than 0.3 centimetre is crushed to pulverizer, then by the kitchen garbage after crushing with de-
Water dispenser is separated into dehydration siccative and oil water mixture two parts.Oil water mixture is discharged into oil water separator progress water-oil separating to obtain
To de-oiling sewage.De-oiling sewage is mixed with dehydration siccative, solid content is 15%, VS/TS=95.3%；Complete the pre- of kitchen garbage
Processing.
（2）Seed sludge：The excess sludge of the first sewage treatment plant of Changsha secondary precipitation discharge is derived from, it is after testing, remaining
Microorganism in sludge is mainly hydrolysis and fermented type microorganism；The specially fusobacterium of obligate anaerobic, Bacteroides, butyric acid arc
Pseudomonas, Bifidobacterium etc..Excess sludge is subjected to Anaerobic culturel one month, the temperature of Anaerobic culturel is 35~45 DEG C, pH controls
System is 7.0 ± 0.5, and anaerobism mixing speed is 100~130 r/min, and a nitrogen source and carbon source are added daily（Carbon source be 300 ±
15mgCOD/L sodium acetate, nitrogen source is 30 ± 1mgNH4 +- N/L ammonium chloride）, sludge age controlled at 12 days, thus Anaerobic culturel
Condition obtains moisture content 99%, the seed sludge of pH=7.0 ± 0.5.
（3）Anaerobic fermentation：Step is passed through with 1.8L（1）The kitchen garbage and 0.2L of pretreatment pass through step（2）Inoculation is dirty
The reaction vessel that mud is made as fermentation substrate, input organic matter glass, nitrogen charging gas drive oxygen carries out anaerobic fermentation.Control anaerobism hair
The temperature of ferment is 37 DEG C, and initial pH is 7 ± 0.5, and speed of agitator is 100~130 r/min.
When being 6 days between when fermenting, the content for determining short chain volatile aliphatic acid in zymotic fluid is 8.4g COD/L（To change
Stoichiometric number is learned to calculate）.
Comparative example 2：
（1）Pre-process kitchen garbage：By wooden chopstick in kitchen garbage, napkin paper, glass ceramics piece and bone, fishbone etc.
The material for being unfavorable for fermentation is rejected.Then less than 0.3 centimetre is crushed to pulverizer, then by the kitchen garbage after crushing with de-
Water dispenser is separated into dehydration siccative and oil water mixture two parts.Oil water mixture is discharged into oil water separator progress water-oil separating to obtain
To de-oiling sewage.De-oiling sewage is mixed with dehydration siccative, solid content is 15%, VS/TS=95.3%；Complete the pre- of kitchen garbage
Processing.
（2）Seed sludge：The excess sludge of the first sewage treatment plant of Changsha secondary precipitation discharge is derived from, it is after testing, remaining
Microorganism in sludge is mainly hydrolysis and fermented type microorganism；The specially fusobacterium of obligate anaerobic, Bacteroides, butyric acid arc
Pseudomonas, Bifidobacterium etc..Excess sludge is subjected to Anaerobic culturel one month, the temperature of Anaerobic culturel is 35~45 DEG C, pH controls
System is 7.0 ± 0.5, and anaerobism mixing speed is 100~130 r/min, and a nitrogen source and carbon source are added daily（Carbon source be 300 ±
15mgCOD/L sodium acetate, nitrogen source is 30 ± 1mgNH4 +- N/L ammonium chloride）, sludge age controlled at 12 days, thus Anaerobic culturel
Condition obtains moisture content 99%, the seed sludge of pH=7.0 ± 0.5.
（3）Anaerobic fermentation：Pass through step with 1.8 L（1）The kitchen garbage and 0.2L of pretreatment pass through step（2）Inoculation is dirty
The reaction vessel that mud is made as fermentation substrate, input organic matter glass, nitrogen charging gas drive oxygen carries out anaerobic fermentation.Control anaerobism hair
The temperature of ferment is 37 DEG C, and initial pH is 7 ± 0.5, and speed of agitator is 100~130 r/min.
When being 14 days between when fermenting, the content for determining short chain volatile aliphatic acid in zymotic fluid is 12.0g COD/L（With
Stoichiometric number is calculated）.
From embodiment 1 to embodiment 5, biosurfactant alkyl has been added in the fermentation system of kitchen garbage
Many glycosides, make the organic matter in kitchen garbage be utilized well, significantly improve the yield of short chain volatile aliphatic acid.
Comparative example 3：
（1）Pre-process kitchen garbage：By wooden chopstick in kitchen garbage, napkin paper, glass ceramics piece and bone, fishbone etc.
The material for being unfavorable for fermentation is rejected.Then less than 0.3 centimetre is crushed to pulverizer, then by the kitchen garbage after crushing with de-
Water dispenser is separated into dehydration siccative and oil water mixture two parts.Oil water mixture is discharged into oil water separator progress water-oil separating to obtain
To de-oiling sewage.De-oiling sewage is mixed with dehydration siccative, solid content is 15%, VS/TS=95.3%；Complete the pre- of kitchen garbage
Processing.
（2）Seed sludge：The excess sludge of the first sewage treatment plant of Changsha secondary precipitation discharge is derived from, it is after testing, remaining
Microorganism in sludge is mainly hydrolysis and fermented type microorganism；The specially fusobacterium of obligate anaerobic, Bacteroides, butyric acid arc
Pseudomonas, Bifidobacterium etc..Excess sludge is subjected to Anaerobic culturel one month, the temperature of Anaerobic culturel is 35~45 DEG C, pH controls
System is 7.0 ± 0.5, and anaerobism mixing speed is 100~130 r/min, and a nitrogen source and carbon source are added daily（Carbon source be 300 ±
15mgCOD/L sodium acetate, nitrogen source is 30 ± 1mgNH4 +- N/L ammonium chloride）, sludge age control was at 12 days, and thus anaerobism is trained
Foster condition obtains moisture content 99%, the seed sludge of pH=7.0 ± 0.5.
（3）Anaerobic fermentation：Step is passed through with 1.8L（1）The kitchen garbage and 0.2L of pretreatment pass through step（2）Inoculation is dirty
The reaction vessel that mud is made as fermentation substrate, input organic matter glass, then puts into total solid in fermentation substrate（TS）Ratio
Weight is 0.2g/g TS rhamnolipid（Biosurfactant）, nitrogen charging gas drive oxygen progress anaerobic fermentation.Control anaerobic fermentation
Temperature is 35~45 DEG C, and initial pH is 7 ± 0.5, and speed of agitator is 100~130 r/min.
When being 6 days between when fermenting, the content for determining short chain volatile aliphatic acid in zymotic fluid is 21.3 g COD/L（With
Stoichiometric number is calculated）.
Comparative example 4：
（1）Pre-process kitchen garbage：By wooden chopstick in kitchen garbage, napkin paper, glass ceramics piece and bone, fishbone etc.
The material for being unfavorable for fermentation is rejected.Then less than 0.3 centimetre is crushed to pulverizer, then the kitchen garbage after crushing is used
Dewaterer is separated into dehydration siccative and oil water mixture two parts.Oil water mixture is discharged into oil water separator and carries out water-oil separating
Obtain de-oiling sewage.De-oiling sewage is mixed with dehydration siccative, solid content is 15%, VS/TS=95.3%；Complete kitchen garbage
Pretreatment.
（2）Seed sludge：The excess sludge of the first sewage treatment plant of Changsha secondary precipitation discharge is derived from, it is after testing, remaining
Microorganism in sludge is mainly hydrolysis and fermented type microorganism；The specially fusobacterium of obligate anaerobic, Bacteroides, butyric acid arc
Pseudomonas, Bifidobacterium etc..Excess sludge is subjected to Anaerobic culturel one month, the temperature of Anaerobic culturel is 35~45 DEG C, pH controls
System is 7.0 ± 0.5, and anaerobism mixing speed is 100~130 r/min, and a nitrogen source and carbon source are added daily（Carbon source be 300 ±
15mgCOD/L sodium acetate, nitrogen source is 30 ± 1mgNH4 +- N/L ammonium chloride）, sludge age controlled at 12 days, thus Anaerobic culturel
Condition obtains moisture content 99%, the seed sludge of pH=7.0 ± 0.5.
（3）Anaerobic fermentation：Pass through step with 1.8 L（1）The kitchen garbage and 0.2L of pretreatment pass through step（2）Inoculation is dirty
The reaction vessel that mud is made as fermentation substrate, input organic matter glass, then puts into total solid in fermentation substrate（TS）Ratio
Weight is 0.2g/g TS saponin（Biosurfactant）, nitrogen charging gas drive oxygen progress anaerobic fermentation.Control the temperature of anaerobic fermentation
For 35~45 DEG C, initial pH is 7 ± 0.5, and speed of agitator is 100~130 r/min.
When being 6 days between when fermenting, the content for determining short chain volatile aliphatic acid in zymotic fluid is 19.6 g COD/L（With
Stoichiometric number is calculated）.
Comparative example 5：
（1）Pre-process kitchen garbage：By wooden chopstick in kitchen garbage, napkin paper, glass ceramics piece and bone, fishbone etc.
The material for being unfavorable for fermentation is rejected.Then less than 0.3 centimetre is crushed to pulverizer, then by the kitchen garbage after crushing with de-
Water dispenser is separated into dehydration siccative and oil water mixture two parts.Oil water mixture is discharged into oil water separator progress water-oil separating to obtain
To de-oiling sewage.De-oiling sewage is mixed with dehydration siccative, solid content is 15%, VS/TS=95.3%；Complete the pre- of kitchen garbage
Processing.
（2）Seed sludge：The excess sludge of the first sewage treatment plant of Changsha secondary precipitation discharge is derived from, it is after testing, remaining
Microorganism in sludge is mainly hydrolysis and fermented type microorganism；The specially fusobacterium of obligate anaerobic, Bacteroides, butyric acid arc
Pseudomonas, Bifidobacterium etc..Excess sludge is subjected to Anaerobic culturel one month, the temperature of Anaerobic culturel is 35~45 DEG C, pH controls
System is 7.0 ± 0.5, and anaerobism mixing speed is 100~130r/min, and a nitrogen source and carbon source are added daily（Carbon source be 300 ±
15mgCOD/L sodium acetate, nitrogen source is 30 ± 1mgNH4 +- N/L ammonium chloride）, sludge age controlled at 12 days, thus Anaerobic culturel
Condition obtains moisture content 99%, the seed sludge of pH=7.0 ± 0.5.
（3）Amphimicrobian ferments：Pass through step with 1.8 L（1）The kitchen garbage and 0.2L of pretreatment pass through step（2）Connect
The reaction vessel that sludge is made as fermentation substrate, input organic matter glass is planted, total solid in fermentation substrate is then put into（TS）
Proportion be 0.2g/g TS alkyl polyglycoside, carry out amphimicrobian fermentation, control amphimicrobian fermentation process in air intake
For 0.60~0.8 m3/ h, ventilatory cycle is 10~15 min/h, and temperature is 35~45 DEG C, and initial pH is 7 ± 0.5, and stirring turns
Speed is 100~130 r/min.
When being 6 days between when fermenting, the content for determining short chain volatile aliphatic acid in zymotic fluid is 12.5 g COD/L（With
Stoichiometric number is calculated）.
The above described is only a preferred embodiment of the present invention, not making any formal limitation to the present invention.Though
So the present invention is disclosed as above with preferred embodiment, but is not limited to the present invention.It is any to be familiar with those skilled in the art
Member, in the case where not departing from the Spirit Essence and technical scheme of the present invention, all using in the methods and techniques of the disclosure above
Appearance makes many possible variations and modification to technical solution of the present invention, or is revised as the equivalent embodiment of equivalent variations.Therefore,
Every content without departing from technical solution of the present invention, the technical spirit according to the present invention is to made for any of the above embodiments any simple
Modification, equivalent substitution, equivalence changes and modification, still fall within technical solution of the present invention protection in the range of.
Claims (7)
1. a kind of method that utilization kitchen garbage produces short chain volatile aliphatic acid, it is characterised in that comprise the following steps：
S1, using kitchen garbage as fermentation substrate, be stirred using sludge as inoculum and be uniformly mixed so as to obtain fermentation substrate；
S2, added into the fermentation substrate anaerobic fermentation is carried out under alkyl polyglycoside, stirring condition；
The sludge is the excess sludge of second pond in sewage treatment plant；Microorganism in the excess sludge is the micro- life of fusobacterium
One or more in thing, Bacteroides microorganism, Butyrivibrio microorganism and Bifidobacterium microorganism belonging to genus；
After the excess sludge is carried out into anaerobic fermentation culture one month, the inoculum in the S1 steps is re-used as.
2. according to the method described in claim 1, it is characterised in that also including kitchen garbage pre-treatment step：
（1）After the kitchen garbage pulverizing, the isolated dehydration siccative of dehydration and oil water mixture are carried out；
（2）Oil water mixture progress deoiling treatment is obtained into de-oiling sewage；
（3）The mixing dehydration siccative and de-oiling sewage.
3. method according to claim 1 or 2, it is characterised in that the addition of the alkyl polyglycoside is the fermentation base
The w/w% of the 5w/w% of solid gross mass in matter~40.
4. method according to claim 1 or 2, it is characterised in that the volume ratio of the fermentation substrate and the inoculum
For 9: 1.
5. method according to claim 1 or 2, it is characterised in that the temperature of anaerobic fermentation is described in the S2 steps
35 DEG C~45 DEG C, fermentation time 4 days~15 days.
6. method according to claim 1 or 2, it is characterised in that the initial pH of anaerobic fermentation described in the S2 steps
For 7 ± 0.5.
7. method according to claim 1 or 2, it is characterised in that the stirring of anaerobic fermentation described in the S2 steps turns
Fast 100r/min~130r/min.
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| CN106801082A (en) * | 2017-02-09 | 2017-06-06 | 广西中烟工业有限责任公司 | A kind of method that utilization tobacco waste produces short chain volatile aliphatic acid |
| CN107812513B (en) * | 2017-10-19 | 2020-08-04 | 界首市锦丰塑业有限公司 | Method for preparing oil stain absorbent by utilizing kitchen and vegetable peel garbage |
| CN109609561A (en) * | 2018-12-06 | 2019-04-12 | 天津科技大学 | A kind of method of wooden carbon source sewage mixed bacteria anaerobism synthesis volatile fatty acid |
| CN110042128A (en) * | 2019-04-30 | 2019-07-23 | 河海大学 | A method of it improving changing food waste into resources and utilizes |
| CN110862207B (en) * | 2019-11-28 | 2021-10-26 | 湖南大学 | Method for promoting degradation of capsaicin in kitchen waste and simultaneously producing methane |
| CN110951789B (en) * | 2019-12-17 | 2023-07-18 | 清华大学深圳国际研究生院 | Kitchen waste treatment method and system |
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| CN104232697A (en) * | 2014-09-10 | 2014-12-24 | 南京理工大学 | Method of producing acid by combining biosurfactant with iron to promote sludge anaerobic fermentation |
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