CN104232697A - Method of producing acid by combining biosurfactant with iron to promote sludge anaerobic fermentation - Google Patents
Method of producing acid by combining biosurfactant with iron to promote sludge anaerobic fermentation Download PDFInfo
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- CN104232697A CN104232697A CN201410459184.8A CN201410459184A CN104232697A CN 104232697 A CN104232697 A CN 104232697A CN 201410459184 A CN201410459184 A CN 201410459184A CN 104232697 A CN104232697 A CN 104232697A
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- anaerobic fermentation
- excess sludge
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Abstract
The invention belongs to the field of solid waste recycling and particularly relates to a method of producing acid by combining a biosurfactant with iron to promote sludge anaerobic fermentation. The method comprises the following steps: adding iron and the biosurfactant into a sludge sample, charging the nitrogen gas into a reactor to expel the oxygen gas, sealing the reactor, uniformly mixing substances of a reaction system, controlling a fermentation temperature and carrying out anaerobic fermentation. By sufficient utilizing the characteristics of the iron and the biosurfactant, the method can be used for effectively reducing the sludge, reducing the pollution on the environment and recycling the sludge to produce short-chain fatty acid with a high utilization value.
Description
Technical field
The invention belongs to solid waste resource recovery field, be specifically related to a kind of method utilizing bio-surfactant alkyl polyglycoside and iron to promote excess sludge fermentation and acid.
Background technology
Biological process creates a large amount of mud while municipal sewage plant is used widely, its output accounts for 0.3 ~ 0.5% (in water ratio 97%) of the process water yield, the sludge yield of quick growth must bring serious secondary pollution problem, and sludge treatment expense is very high, maximumly can account for more than 50% of sewage work's working cost, this has become the significant burden that sewage work runs.In today of economic construction and environment protection synchronized development, conventional specific resistance to filtration mode can not meet the demands, and rational recycling sludge utilizes has become an engineering very important in Environment control problem.
Certain active substance that excess sludge is made up of the organism of suspended substance, colloid or dissolved form in waste water, its overwhelming majority is made up of microbial cells, and wherein organic content is about 60%, and biological easily degraded organic constituent is more than 40%.These biological easily biodegradable organicses mainly protein, polysaccharide etc., they are nutrition sources that microorganism is enriched.The organic matter degradation existed a large amount of in mud can be converted into the renewable energy source such as methane, hydrogen material by anaerobically fermenting lignin-sludge effectively, wherein, short chain fatty acid is as the intermediate product in fermenting process, not only there is higher value, and have a wide range of applications and many potential industrial uses.In addition, in sewage work, short chain fatty acid directly can be used as the interior carbon source of sewage work microorganism, in order to remove the nitrogen phosphorus in sewage.Therefore, if effectively the organism in mud can be converted into short chain fatty acid, to the minimizing of sludge treatment, resource utilization and innoxious, improve the effect of the denitrogenation dephosphorizing of sewage work by realizing simultaneously.
The anaerobically fermenting of mud is divided into hydrolysis, acidifying and methanation three stage, and wherein organic hydrolysis stage is rate-limiting step.Because microbial cell wall can adhere to for a long time not being broken under digestive environments, and in mud, most of organic substance is present in microorganism cells, therefore hydrolysis rate is contained.From production organic acid angle, if the hydrolysis rate of mud can be improved, namely provide the substrate of more voluminous acid, just likely increase organic acid output.Tensio-active agent is extensively deposited and is applied to daily articles for washing, also ubiquity in sanitary sewage, there are some researches show, tensio-active agent has solublization to the organism (mainly protein and soluble sugar) in mud, appropriate tensio-active agent also has crushing effect to microorganism cells under suitable pH condition, this can be mud undoubtedly and produces acid and provide and utilize matrix, and tensio-active agent therefore can be utilized to improve the hydrolysis rate of mud, thus improves mud and produce acid amount.In addition, iron has stronger reductibility, can reduce the redox potential of system, provides the ferro element needed for microorganism, improves the activity of acid-producing microorganisms, thus the anaerobically fermenting improving mud produces the velocity ratio and output of short chain fatty acid.So far, about utilizing tensio-active agent and iron jointly to promote, the research that sludge fermentation produces short chain fatty acid is not yet reported.
Summary of the invention
The object of this invention is to provide a kind of method more effectively promoting the acid of sludge fermentation product, propose in excess sludge, add tensio-active agent and iron, thus promote the method for sludge anaerobic fermentation to produce short chain fatty acid.
The technical solution realizing the object of the invention is: a kind of bio-surfactant and iron promote the method for excess sludge fermentation and acid, have employed alkyl polyglycoside and reduced iron promotes excess sludge anaerobic fermentation and acid production, specifically comprises the following steps:
(1) excess sludge is added in digestion bottle, add alkyl polyglycoside and iron;
(2), after digestion bottle being driven oxygen and filling nitrogen 10min, sealing digestion bottle, constant-temperature shaking culture carries out anaerobically fermenting, completes the process that bio-surfactant alkyl polyglycoside and reduced iron promote excess sludge anaerobic fermentation and acid production.
Wherein, in step (1), excess sludge takes from secondary sedimentation tank of sewage treatment work, and the total solid content of excess sludge is 3.8%.
In step (1), the dosage of alkyl polyglycoside is 0.005 ~ 0.1g/gTSS.
In step (1), the dosage of iron is 0.005 ~ 1g/gTSS.
In step (1), the kind of iron comprises iron plane flower, reduced iron powder etc.
In step (2), fill nitrogen and drive oxygen 10min.
In step (2), the mixing rotating speed in reaction system is 100 ~ 150rpm/min.
In step (2), leavening temperature is 30 ~ 37 DEG C, and fermentation time is 1 ~ 15d.
In step (2), in anaerobic fermentation process, the pH scope of anaerobic fermentation system is 6.0 ~ 8.0.
The present invention compared with prior art, its remarkable advantage: use bio-surfactant and iron, in conjunction with both advantage promote the anaerobic fermentation and acid production of excess sludge to make product acid measure larger raising.On the one hand, take full advantage of the characteristic of bio-surfactant, promote the organism stripping such as undissolved macro-molecular protein and carbohydrate in mud, reinforcement sludge is hydrolyzed, and improves sludge anaerobic fermentation and acid production amount; On the other hand, utilize the features such as the reductibility of iron, promote the activity of enzyme classes in mud, thus improve the fermentation efficiency of mud.
Embodiment
The following examples can make those skilled in the art more fully understand the present invention.
Bio-surfactant and iron promote a method for excess sludge fermentation and acid, it is characterized in that utilizing bio-surfactant reduction surface tension and iron to provide the characteristic of electronics, promote excess sludge anaerobic fermentation and acid production, specifically comprise the following steps:
(1) excess sludge is added in digestion bottle, add alkyl polyglycoside 0.005 ~ 0.1g/gTSS and iron 0.005 ~ 1g/gTSS.；
(2), after digestion bottle being driven oxygen and filling nitrogen 10min, sealing digestion bottle, at 30 ~ 37 DEG C, constant-temperature shaking culture carries out anaerobically fermenting, completes the process that bio-surfactant alkyl polyglycoside and reduced iron promote excess sludge anaerobic fermentation and acid production after 1 ~ 10d.
Embodiment 1:
Excess sludge is added in digestion bottle, supernatant fluid of draining after natural subsidence 24h, obtain excess sludge (PH=6.8) produces short chain fatty acid substrate (following examples are same) as anaerobically fermenting;
In reaction flask, add alkyl polyglycoside and reduced iron powder, consumption is 0.02g/gTSS, 0.05g/gTSS respectively, and after driving oxygen and filling nitrogen 5min, closed reactor carries out anaerobically fermenting.By microbial process in alkyl polyglycoside, reduced iron powder and mud, the organism contained in mud is converted into short chain fatty acid.Wherein control temperature is 35 ± 1 DEG C, and fermentation decorum pH changes in 6.0 ~ 7.0 scopes, and the mud residence time is in the reactor 5d, produces the content 1869.28mg/L of short chain fatty acid.
Embodiment 2:
Excess sludge is added in digestion bottle, supernatant fluid of draining after natural subsidence 24h, obtain excess sludge (PH=6.8) produces short chain fatty acid substrate (following examples are same) as anaerobically fermenting;
In reaction flask, add alkyl polyglycoside and reduced iron powder, consumption is 0.05g/gTSS, 0.005g/gTSS respectively, and after driving oxygen and filling nitrogen 5min, closed reactor carries out anaerobically fermenting.By microbial process in alkyl polyglycoside, reduced iron powder and mud, the organism contained in mud is converted into short chain fatty acid.Wherein control temperature is 35 ± 1 DEG C, and fermentation decorum pH changes in 6.0 ~ 7.0 scopes, and the mud residence time is in the reactor 5d, produces the content 2575.62mg/L of short chain fatty acid.
Embodiment 3:
Excess sludge is added in digestion bottle, supernatant fluid of draining after natural subsidence 24h, obtain excess sludge (PH=6.8) produces short chain fatty acid substrate (following examples are same) as anaerobically fermenting;
In reaction flask, add alkyl polyglycoside and reduced iron powder, consumption is 0.05g/gTSS, 0.05g/gTSS respectively, and after driving oxygen and filling nitrogen 5min, closed reactor carries out anaerobically fermenting.By microbial process in alkyl polyglycoside, reduced iron powder and mud, the organism contained in mud is converted into short chain fatty acid.Wherein control temperature is 35 ± 1 DEG C, and fermentation decorum pH changes in 6.0 ~ 7.0 scopes, and the mud residence time is in the reactor 5d, produces the content 2630.98mg/L of short chain fatty acid.
Embodiment 4:
Excess sludge is added in digestion bottle, supernatant fluid of draining after natural subsidence 24h, obtain excess sludge (PH=6.8) produces short chain fatty acid substrate (following examples are same) as anaerobically fermenting;
In reaction flask, add alkyl polyglycoside and reduced iron powder, consumption is 0.1g/gTSS, 0.005g/gTSS respectively, and after driving oxygen and filling nitrogen 5min, closed reactor carries out anaerobically fermenting.By microbial process in alkyl polyglycoside, reduced iron powder and mud, the organism contained in mud is converted into short chain fatty acid.Wherein control temperature is 35 ± 1 DEG C, and fermentation decorum pH changes in 6.0 ~ 7.0 scopes, and the mud residence time is in the reactor 5d, produces the content 3036.12mg/L of short chain fatty acid.
Embodiment 5:
Excess sludge is added in digestion bottle, supernatant fluid of draining after natural subsidence 24h, obtain excess sludge (PH=6.8) produces short chain fatty acid substrate (following examples are same) as anaerobically fermenting;
In reaction flask, add alkyl polyglycoside and reduced iron powder, consumption is 0.2g/gTSS, 0.05g/gTSS respectively, and after driving oxygen and filling nitrogen 5min, closed reactor carries out anaerobically fermenting.By microbial process in alkyl polyglycoside, reduced iron powder and mud, the organism contained in mud is converted into short chain fatty acid.Wherein control temperature is 35 ± 1 DEG C, and fermentation decorum pH changes in 6.0 ~ 7.0 scopes, and the mud residence time is in the reactor 5d, produces the content 2920.87mg/L of short chain fatty acid.
Embodiment 6:
Excess sludge is added in digestion bottle, supernatant fluid of draining after natural subsidence 24h, obtain excess sludge (pH=6.8) produces short chain fatty acid substrate (following examples are same) as anaerobically fermenting;
In reaction flask, add alkyl polyglycoside and reduced iron powder, consumption is 0.2g/gTSS, 1.0g/gTSS respectively, and after driving oxygen and filling nitrogen 5min, closed reactor carries out anaerobically fermenting.By microbial process in alkyl polyglycoside, reduced iron powder and mud, the organism contained in mud is converted into short chain fatty acid.Wherein control temperature is 35 ± 1 DEG C, and fermentation decorum pH changes in 6.0 ~ 7.0 scopes, and the mud residence time is in the reactor 3d, produces the content 2587.24mg/L of short chain fatty acid.
Comparative example 1:
Excess sludge is added in digestion bottle, supernatant fluid of draining after natural subsidence 24h, obtain excess sludge (pH=6.8) produces short chain fatty acid substrate (following examples are same) as anaerobically fermenting;
In reaction flask, do not add alkyl polyglycoside and reduced iron powder, after driving oxygen and filling nitrogen 5min, closed reactor carries out anaerobically fermenting.By microbial process in mud, the organism contained in mud is converted into short chain fatty acid.Wherein control temperature is 35 ± 1 DEG C, and fermentation decorum pH changes in 6.0 ~ 7.0 scopes, and the mud residence time is in the reactor 5d, produces the content 1489.44mg/L of short chain fatty acid.
Comparative example 2:
Excess sludge is added in digestion bottle, supernatant fluid of draining after natural subsidence 24h, obtain excess sludge (pH=6.8) produces short chain fatty acid substrate (following examples are same) as anaerobically fermenting;
In reaction flask, add alkyl polyglycoside, consumption is 0.2g/gTSS, and after driving oxygen and filling nitrogen 5min, closed reactor carries out anaerobically fermenting.By microbial process in alkyl polyglycoside and mud, the organism contained in mud is converted into short chain fatty acid.Wherein control temperature is 35 ± 1 DEG C, and fermentation decorum pH changes in 6.0 ~ 7.0 scopes, and the mud residence time is in the reactor 4d, produces the content 2367.33mg/L of short chain fatty acid.
Comparative example 3:
Excess sludge is added in digestion bottle, supernatant fluid of draining after natural subsidence 24h, obtain excess sludge (pH=6.8) produces short chain fatty acid substrate (following examples are same) as anaerobically fermenting;
In reaction flask, add reduced iron powder, consumption is 1g/gTSS, and after driving oxygen and filling nitrogen 5min, closed reactor carries out anaerobically fermenting.By microbial process in mud, the organism contained in mud is converted into short chain fatty acid.Wherein control temperature is 35 ± 1 DEG C, and fermentation decorum pH changes in 6.0 ~ 7.0 scopes, and the mud residence time is in the reactor 4d, produces the content 1314.28mg/L of short chain fatty acid.
Claims (8)
1. bio-surfactant combines the method promoting excess sludge anaerobic fermentation and acid production with reduced iron, it is characterized in that, have employed alkyl polyglycoside and reduced iron promotes excess sludge anaerobic fermentation and acid production, specifically comprises the following steps:
(1) excess sludge is added in digestion bottle, add alkyl polyglycoside and iron;
(2), after digestion bottle being driven oxygen and filling nitrogen 10min, sealing digestion bottle, constant-temperature shaking culture carries out anaerobically fermenting, completes the process that bio-surfactant alkyl polyglycoside and reduced iron promote excess sludge anaerobic fermentation and acid production.
2. bio-surfactant according to claim 1 combines the method promoting excess sludge anaerobic fermentation and acid production with reduced iron, it is characterized in that, in step (1), the dosage of alkyl polyglycoside is 0.005 ~ 0.1g/gTSS.
3. bio-surfactant according to claim 1 combines the method promoting excess sludge anaerobic fermentation and acid production with reduced iron, it is characterized in that, in step (1), the dosage of iron is 0.005 ~ 1g/gTSS.
4. bio-surfactant according to claim 1 combines the method promoting excess sludge anaerobic fermentation and acid production with reduced iron, it is characterized in that, in step (1), the kind of iron comprises iron plane flower, reduced iron powder etc.
5. bio-surfactant according to claim 1 combines the method promoting excess sludge anaerobic fermentation and acid production with reduced iron, it is characterized in that, in step (2), fills nitrogen and drives oxygen 10min.
6. bio-surfactant according to claim 1 combines the method promoting excess sludge anaerobic fermentation and acid production with reduced iron, it is characterized in that, in step (2), the mixing rotating speed in reaction system is 100 ~ 150rpm/min.
7. bio-surfactant according to claim 1 combines the method promoting excess sludge anaerobic fermentation and acid production with reduced iron, it is characterized in that, in step (2), leavening temperature is 30 ~ 37 DEG C, and fermentation time is 1 ~ 15d.
8. bio-surfactant according to claim 1 combines the method promoting excess sludge anaerobic fermentation and acid production with reduced iron, it is characterized in that, in step (2), in anaerobic fermentation process, the pH scope of anaerobic fermentation system is 6.0 ~ 8.0.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104498541A (en) * | 2014-12-30 | 2015-04-08 | 湖南大学 | Method for producing short-chain volatile fatty acids by utilizing kitchen wastes and short-chain volatile fatty acids |
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- 2014-09-10 CN CN201410459184.8A patent/CN104232697A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| JINGYANG LUO，: "Stimulating short-chain fatty acids production from waste activated sludge by nano zero-valent iron，", 《JOURNAL OF BIOTECHNOLOGY》 * |
| YAOBIN ZHANG: "Enhanced high-solids anaerobic digestion of waste activated sludge by the addition of scrap iron", 《BIORESOURCE TECHNOLOGY》 * |
| 陈灿: "烷基多苷促进污泥水解产酸的研究", 《环境科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104498541A (en) * | 2014-12-30 | 2015-04-08 | 湖南大学 | Method for producing short-chain volatile fatty acids by utilizing kitchen wastes and short-chain volatile fatty acids |
| CN104498541B (en) * | 2014-12-30 | 2017-09-29 | 湖南大学 | The method and short chain volatile aliphatic acid of short chain volatile aliphatic acid are produced using kitchen garbage |
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Application publication date: 20141224 |