CN102311935A - Controlled release microecological preparation for water quality improvement and preparation method thereof - Google Patents
Controlled release microecological preparation for water quality improvement and preparation method thereof Download PDFInfo
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- CN102311935A CN102311935A CN 201110248023 CN201110248023A CN102311935A CN 102311935 A CN102311935 A CN 102311935A CN 201110248023 CN201110248023 CN 201110248023 CN 201110248023 A CN201110248023 A CN 201110248023A CN 102311935 A CN102311935 A CN 102311935A
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- subtilis
- probiotics
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Abstract
The invention which discloses a controlled release microecological preparation for water quality improvement and a preparation method thereof belongs to the biotechnological field, and concretely relates to the preparation method of the controlled release microecological preparation for water quality improvement. The microecological preparation treats bacillus subtilis as a main flora and Bacillus cereus, lactics, and microzyme as auxiliary floras. By adopting the composite microbial preparation to process water, organic mud can be effectively degraded, harmful substances of ammonia, nitrites, hydrogen sulfide and the like in water can be absorbed and utilized, the breeding of harmful microbes and harmful algae can be inhibited, the microecological environment of water can be balanced, and the health growth of aquatic organisms can be promoted, so the microecological preparation of the present invention is more economical and is more suitable for popularization than common microecological preparations.
Description
Technical field
The present invention relates to a kind of water quality modifying microecological preparation and preparation method thereof that is used for, especially a kind of control release type probiotics.
Technical background
At present, China's water environment pollution is quite serious, and in the national seven great river River system, 40% left and right sides water quality is inferior to five class standards; Eutrophication in various degree appears in the lake in the whole nation 75%; The section, city is polluted outstanding; Area, coastal river mouth and surrounding waters, city are polluted seriously polluted, and the red tide generation frequency increases, enlarged areas.The safety of urban drinking water and the people's health in the pollution of water surrounding direct threats.So, contaminated rivers and lakes water body is repaired, be pressing for of socio-economic development and improvement of the ecological environment.
The mikrobe water body purification is the biologics that is specifically designed to rivers and lakes water body purification, waste water control and reparation; Form by tens kinds of beneficial microbe colonies; Be to adopt the biotechnology means that natural microorganisms is carried out the efficient special microorganism that modification, reinforcement, acclimation shaking culture form; Contained mikrobe is and from physical environment, filters out a series of specialization mikrobe, and is more effective than the mikrobe under state of nature, can set up living weight through natural mechanism; Can improve the water body quality rapidly, reach the effect of best purification water quality.
In March, 2000, the natto flora (friendly mikrobe) that Japanese scientist will be specifically designed to purification of water quality directly has been written into success for the first time in the special cement.So, employed some cement products that are prone to pollute environment just can become the environmental purification type novel material with new function at present.Bacillus natto specially to water pollution with prevent water quality deterioration, prevent the water-purifying material of problems such as water quality bounce-back, as long as have water and oxygen to breed in a large number, reach the purpose that forever purifies water.Overcome traditional improvement method and be prone to bounce-back, be prone to shortcomings such as secondary pollution, maintenance cost height, directly solved a difficult problem of constantly regularly throwing in bacteria agent, can reach non-maintaining for a long time, exempt from bounce-back, use ideal effects such as simple (directly throwing in), instant effect.
The microniological proudcts of U.S. Alear-Murray company are processed after concentrating dormancy by multiple mikrobe, and along with the metabolism of mikrobe, the C in the former system, N are decomposed into the gas evolution waters surface such as CO2, CH4 improves water quality.In recent years, the quality recovery, the pollution treatment of New York's Central Park lake that have been applied to desilting, the U.S. aquarium of Thailand's aquaculture pond of this technology removed projects such as algae, and all achieved success.
The application of domestic present useful microbe aspect water purification accepted and paid attention to day by day, but research is only in the starting stage.In application facet, domestic stand-alone development mainly be some single bacterial strains, like photosynthetic bacterium, genus bacillus, Bdellovibrio etc.; Compound formulation mainly is imitated or introduces external commodity, and majority is data, the research means of more science also of no use and content role of evaluation mechanism and result of use to aspects such as growth velocity, bait transformation efficiency, survival rates.
Summary of the invention
Technical problem to be solved by this invention provides a kind of control release type probiotics, to solve the problem of water quality improvement.
For solving the problems of the technologies described above, technical scheme of the present invention is: with subtilis is main flora, is auxiliary flora configuration probiotics with Bacillus cereus, milk-acid bacteria, yeast; Wherein the subtilis powder accounts for 60-80%, and Bacillus cereus accounts for 10-12%, and milk-acid bacteria accounts for 0-10%, and yeast accounts for 5-15%.
The substratum that said subtilis is used is sucrose 0.5%, MgSO
40.02%, CaCO
30.5%, K
2HPO
40.02%, pH7.0～7.2.
The condition of said subtilis seed culture is: 500 milliliters of triangular flask liquid amounts are 100 milliliters, and 121 ℃ of sterilizations of high temperature 20 minutes connect inclined-plane one ring after the cooling; Shaking table is cultivated; Shaking speed is 200 rev/mins, 37 ℃ of culture temperature, incubation time 20-28 hour.
The fermention medium of said subtilis is: sucrose 1%, yeast extract paste 0.02%, MgSO
40.01%, Na
2HPO
40.01%, CaCO
30.1%, pH 7.0.
The fermentation condition of said subtilis is: liquid amount is 800L in 1 ton of jar, and temperature 30-37 ℃, stirring velocity is 150-250 rev/min, air flow 1-3 cube/minute, cultivates 15-30 hour.The content of subtilis is 8 * 10 during fermentation ends
11-1 * 10
12Individual/milliliter bacterium liquid.
Said probiotics is solid-state, and matrix adopts nano-porous materials, preferred silicon-dioxide.
Because of its particle diameter is very little, specific surface area is big, and surface adsorption power is strong; Aspects such as surface energy is big, and dispersing property is good have special performance, and viable bacteria is lived away from home in the porous; Reduce the influence of external environment, in water body, can slowly discharge thalline, prolong the clarification time of thalline water quality to bacterium.
The present invention utilizes fine inorganic novel material silicon-dioxide, and because of its particle diameter is very little, specific surface area is big; Surface adsorption power is strong; Surface energy is big, and viable bacteria is had the characteristic of very strong absorption and provide protection, is adsorbed on subtilis in the nano silicon; Mix with Bacillus cereus, milk-acid bacteria, yeast according to a certain percentage dry back, processes a kind of control release type probiotics and be used for the water quality improvement product.In water quality improvement, under the equal conditions, this kind product and water body contact area are big, and the water quality treatment volume is many relatively, and usage quantity reduces relatively.Characteristics such as the control release type probiotics has long action time in water quality improvement, decomposing organic matter efficient is high, and the viable bacteria reproduction speed is fast.Often use these article can effectively improve water quality, the residual bait of promotion and other harmful and noxious substances such as organic decomposition, degradation of ammonia nitrogen, nitrite, hydrogen sulfide that float, increase oxygen in water, promote useful planktonic growth in the water body.
Embodiment
The preparation of embodiment 1 control release type probiotics
1, the seed culture of subtilis
The seed culture medium of subtilis is: sucrose 0.5%, MgSO
40.02%, CaCO
30.5%, K
2HPO
40.02%, pH7.0～7.2.500 milliliters of triangular flask liquid amounts are 100 milliliters, and 121 ℃ of sterilizations of high temperature, 20 packing connect inclined-plane one ring after the cooling, and shaking table is cultivated, and shaking speed is 200 rev/mins, 37 ℃ of culture temperature, incubation time 28 hours.
2, the production of subtilis
The liquid nutrient medium of subtilis is a sucrose 1%, yeast extract paste 0.02%, MgSO
40.01%, Na
2HPO
40.01%, CaCO
30.1%, pH 7.0.Fermentor tank is poured substratum into after sky disappeared 1 hour in advance, in 1 ton of jar, and dress 800L fermented liquid, 121-125 ℃ of sterilization 1 hour is cooled to 30-35 ℃, inoculation.
Fermentation condition is: 37 ℃ of leavening temperatures, stirring velocity are 250 rev/mins, and 3 cubes/minute of air flows were cultivated 20 hours.The content of subtilis is 9 * 10 during fermentation ends
11Individual/milliliter bacterium liquid.
3, control release type subtilis powder preparation
During fermentation ends, according to the ratio of 1 kilogram of silicon-dioxide absorption 1L fermented liquid, solid-liquid mixes, and gets into low temperature drying room (50 degree) and dries to moisture below 10%, and about 5 hours, the bacterium powder was crossed behind 100 mesh sieves for use, and detecting back bacillus subtilis bacterial content is 7 * 10
11Individual/gram.
4, composite bacteria preparation
Commercial bacillus cereus, milk-acid bacteria, yeast, (activity of above bacterial classification is 1-2 * 10
8Individual/gram), wherein Bacillus cereus accounts for 10%, milk-acid bacteria 5%, yeast accounts for 5%, mixes with control release type subtilis powder 80%.
The preparation of embodiment 2 control release type probioticses
The preparation method is said identical with embodiment 1, and different is that the composite bacteria ratio is different.Control release type subtilis powder 60%, bacillus cereus 15%, milk-acid bacteria 10%, yeast 15%.
The preparation of embodiment 3 control release type probioticses
The preparation method is said identical with embodiment 1, and different is that the composite bacteria ratio is different.Control release type subtilis powder 75%, bacillus cereus 12%, milk-acid bacteria 7%, yeast 6%.
Embodiment 4, control release type probiotics application test
Get 10 gram control release type probiotics bacterium powder and be scattered in the water 10L that needs purification, aerobic culture is about 14 hours, and full pool spilling head is perhaps mixed the back full pool spilling head thoroughly with feed, and 10 gram bacterium powder are with 1 mu of pond.
The same day after using, need open oxygen increasing pump ventilation 2-5 hour, increase the dissolved oxygen in water degree, promote the subtilis breeding; Opened every other day later on topping-up pump 1-2 hour.Ammonia nitrogen, COD, DO and water body PH in the continuous detecting 7 days, every day sampling and measuring water body.The water quality physical and chemical index adopts the intelligent Water Test Kits of the full-automatic online Water Test Kits of Australia's gram force to measure.
Table is surveyed in the health check-up of table 1 test water
Can find out from table 1 result, the improve significantly effect of water quality of the probiotics of the present invention test, each item index all is superior to the similar commodity of commercially available certain brand that contrast, compares with blank, embodies this probiotics specific function.
The activation analysis of embodiment 5 compound micro-ecological preparations
Get compound micro-ecological preparation 1 gram, carry out conventional live bacterial count.The result show various bacterium viable bacterias all 5,000,000,000/more than the gram, obviously be superior to commercially available like product at present, and the survival rate after a year is more than 80%.
More than shown and described ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should understand; The present invention is not restricted to the described embodiments; Description in the foregoing description and the specification sheets principle of the present invention just is described, under the prerequisite that does not break away from spirit and scope of the invention, the present invention also has various changes and modifications; These variations and improvement all fall in the scope of the invention that requires protection, and the present invention requires interior appending claims of protection domain and equivalent thereof to define.
Claims (8)
1. a control release type probiotics that is used for water quality improvement is characterized in that with subtilis be main flora, is the probiotics of auxiliary flora with Bacillus cereus, milk-acid bacteria, yeast.
2. the described probiotics of claim 1 is characterized in that the subtilis powder accounts for 60-80%, and Bacillus cereus accounts for 10-15%, and milk-acid bacteria accounts for 0-10%, and yeast accounts for 5-15%.
3. claim 1 or 2 said probioticses is characterized in that the substratum that said subtilis is used is sucrose 0.5 %, MgSO
40. 02 %, CaCO
30.5 %, K
2HPO
40.02 %, pH7.0～7.2.
4. according to the said probiotics of claim 3; The condition that it is characterized in that said subtilis seed culture is: 500 milliliters of triangular flask liquid amounts are 100 milliliters, and 121 ℃ of sterilizations of high temperature 20 minutes connect inclined-plane one ring after the cooling; Shaking table is cultivated; Shaking speed is 200 rev/mins, 37 ℃ of culture temperature, incubation time 20-28 hour.
5. according to the said probiotics of claim 3, it is characterized in that the fermention medium of said subtilis is: sucrose 1 %, yeast extract paste 0.02%, MgSO
40.01 %, Na
2HPO
40. 01 %, CaCO
30.1 %, pH 7. 0.
6. according to the said probiotics of claim 3; The fermentation condition that it is characterized in that said subtilis is: liquid amount is 800L in 1 ton of jar; Temperature 30-37 ℃, stirring velocity is 150-250 rev/min, air flow 1-3 cube/minute; Cultivated 15-30 hour, the content of subtilis is 8 * 10 during fermentation ends
11-1 * 10
12Individual/milliliter bacterium liquid.
7. according to claim 1 or 2 said probioticses, its characteristic with said probiotics be solid-state, matrix adopts nano-porous materials.
8. according to the said probiotics of claim 6, it is characterized in that said nano-porous materials is fine inorganic novel material silicon-dioxide.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103695344A (en) * | 2013-12-16 | 2014-04-02 | 大连海洋大学 | Bacillus proliferation promoting mineral substance regulator used for fermentation |
| CN103708694A (en) * | 2014-01-08 | 2014-04-09 | 昆山工研院华科生物高分子材料研究所有限公司 | Microorganism dredging method and device |
| CN104496035A (en) * | 2014-11-20 | 2015-04-08 | 杨凌华辕生物产业集团有限公司 | Microecologic prepation used for water-soil environment security and preparation method thereof |
| CN105836900A (en) * | 2016-06-03 | 2016-08-10 | 北京荣蒂盛环境科技有限公司 | Ecological water purifying system |
| CN109777751A (en) * | 2019-01-21 | 2019-05-21 | 四川清和福生生物工程有限公司 | A kind of plant growth-promoting bacteria slow-releasing granules and its preparation method and application |
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| CN101109008A (en) * | 2006-07-18 | 2008-01-23 | 上海四季生物科技有限公司 | Aquifer amendment containing multiple active microorganisms and method of preparing the same |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103695344A (en) * | 2013-12-16 | 2014-04-02 | 大连海洋大学 | Bacillus proliferation promoting mineral substance regulator used for fermentation |
| CN103708694A (en) * | 2014-01-08 | 2014-04-09 | 昆山工研院华科生物高分子材料研究所有限公司 | Microorganism dredging method and device |
| CN104496035A (en) * | 2014-11-20 | 2015-04-08 | 杨凌华辕生物产业集团有限公司 | Microecologic prepation used for water-soil environment security and preparation method thereof |
| CN105836900A (en) * | 2016-06-03 | 2016-08-10 | 北京荣蒂盛环境科技有限公司 | Ecological water purifying system |
| CN109777751A (en) * | 2019-01-21 | 2019-05-21 | 四川清和福生生物工程有限公司 | A kind of plant growth-promoting bacteria slow-releasing granules and its preparation method and application |
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| CN102311935B (en) | 2013-05-22 |
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Address after: 310012 , 164 Wen two road, Hangzhou, Zhejiang, Hangzhou Institute of Commerce Patentee after: ZHEJIANG SHUANGLIANG SHANGDA ENVIRONMENTAL PROTECTION Co.,Ltd. Address before: 310018 , 164 Wen two road, Hangzhou, Zhejiang, Hangzhou Institute of Commerce Patentee before: ZHEJIANG SUNDA ENVIRONMENTAL PROTECTION Co.,Ltd. |
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Address after: Inside Hangzhou Business School, No.164 Wen'er Road, Xihu District, Hangzhou City, Zhejiang Province, 310012 Patentee after: Zhe JIANG Shuangliang Sunda Environment Protection Co.,Ltd. Address before: Inside Hangzhou Business School, No.164 Wen'er Road, Xihu District, Hangzhou City, Zhejiang Province, 310012 Patentee before: ZHEJIANG SHUANGLIANG SHANGDA ENVIRONMENTAL PROTECTION Co.,Ltd. |