CN109319943A - Black smelly water harnessing microbial inoculum based on quorum sensing and the preparation method and application thereof - Google Patents
Black smelly water harnessing microbial inoculum based on quorum sensing and the preparation method and application thereof Download PDFInfo
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- CN109319943A CN109319943A CN201811151331.XA CN201811151331A CN109319943A CN 109319943 A CN109319943 A CN 109319943A CN 201811151331 A CN201811151331 A CN 201811151331A CN 109319943 A CN109319943 A CN 109319943A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The present invention relates to technical field of sewage, and in particular to a kind of black smelly water harnessing microbial inoculum and the preparation method and application thereof based on quorum sensing.Black smelly water harnessing microbial inoculum is made of the component of following parts by weight: black 30~50 parts of smelly water harnessing bacterium mud, 50~70 parts of drying protectant, 0.001~0.1 part of colony induction signaling molecule compound, preparation method includes the following steps: (1) black smelly water harnessing bacterium mud is made；(2) the centrifugation supernatant bacterium solution of Ha Weishi vibrios BB170 is made；(3) black smelly water harnessing microbial inoculum is made.Black and odorous water, which administers microbial inoculum, not only to be had to the efficient processing capacity of black and odorous water; biomembrane is quickly formed in black and odorous water bottom sludge can be realized the improvement permanently effective to black and odorous water simultaneously; its production process is at low cost; apparatus and process is easy to operate; industrial scale production can be achieved, there is significant environment and economic benefit.
Description
Technical field
The present invention relates to technical field of sewage, and in particular to a kind of black smelly water harnessing microbial inoculum based on quorum sensing
And the preparation method and application thereof.
Background technique
In Rapid-Urbanization and industrialized process, since cities and towns infrastructure seriously lags, urban river falls
Concentrate the main thoroughfare of blowdown for industrial wastewater, sanitary sewage and farmland water-break, pollutant discharge amount is big and spatial distribution collection
In, only than high, water body self-purification ability is weak for river dirt, be easy to cause anoxic and eutrophication, forms black and odorous water.Urban black is smelly
Water body has become a kind of urban disease, and all cities are escaped by luck almost without one.Black and odorous water can discharge H into environment2S and NH3Deng
Foul gas, while mosquito and pathogenic bacteria are bred, it is serious to destroy people's lives environment and threaten human health.
Currently, the reparation in medium and small city and small towns black and odorous water has become the hot issue being concerned.
Microorganism enhanced technology is a kind of effective black and odorous water recovery technique, has at low cost, easy to operate, nothing two
The advantages that secondary pollution, purification of water quality is significant.But the microbial bacterial agent due to adding can only be invested in static river.Once
River water restores to flow again, and the effective concentration for adding microorganism will quickly lower, and leads to not especially black smelly to water body
Bed mud carries out permanently effective processing.Microorganism enhanced technology is eventually led in black smelly water reparation field, is generally used for small-sized
Water body is closed, large-scale application is unsuitable for.
Summary of the invention
In order to overcome shortcoming and defect existing in the prior art, the purpose of the present invention is to provide one kind for black smelly
The smelly water harnessing microbial inoculum of efficient black that water body overlying water and bottom sludge are administered and cut down.
It is a further object of the present invention to provide the preparation method of black smelly water harnessing microbial inoculum, microorganism obtained by this method
Microbial inoculum can be effectively treated black and odorous water overlying water, while by Microorganism colonization in bed mud, realize to black smelly water
The permanently effective processing of body bed mud, simple process is efficient, is conducive to industrial production.
It is black smelly in improvement that another mesh of the invention is to provide the black smelly water harnessing bacterial preparation process based on quorum sensing
The application of water.
The purpose of the invention is achieved by the following technical solution:
A kind of black smelly water harnessing microbial inoculum based on quorum sensing, is made: black smelly water harnessing bacterium of the component of following parts by weight
30~50 parts of mud, 50~70 parts of drying protectant, 0.001~0.1 part of colony induction signaling molecule compound.
Microbial inoculum of the present invention uses based on black smelly water harnessing bacterium mud, and black smelly water harnessing bacterium mud includes black and odorous water efficient process
Microorganism, while being aided with a variety of corresponding micropopulation induction signal molecules, black and odorous water efficient process microorganism is by dividing
The signaling molecule in environment is secreted and incuded, the exchange of the information between microorganism is realized, and regulates and controls the row of part microorganism
For.Microbial inoculum of the present invention not only has to the efficient processing capacity of black and odorous water, while the quick shape in black and odorous water bottom sludge
At biomembrane, have higher anti-current-rush ability, can be realized the improvement permanently effective to black and odorous water.
Further, effective biomass of the black smelly water harnessing microbial inoculum is 1 × 107~1 × 1010CFU/g。
Further, the black smelly water harnessing bacterium mud preparation method the following steps are included: by black and odorous water administer strain into
Row activation, be inoculated into LB culture medium, 36~38 DEG C culture 23-25 hours, gained bacterium solution is centrifuged, supernatant is pipetted
Liquid is collected remaining bacterium mud, is dried, and the drying temperature is 25~35 DEG C, 0.1~0.3MPa of pressure, dry 6~12h,
The black smelly water harnessing bacterium mud of the bacterium for being made stand-by.
Further, the black smelly water harnessing strain includes following components: acinetobacter (Acinetobacter
Sp.), bacillus (Bacillus sp.), pseudomonas (Pseudomonas sp.), anaerobism Bacillus adhaerens category
(Anaeromyxobacter sp.), alcaligenes (Alcaligenes sp.), wild jujube Pseudomonas (Acidovorax sp.)
And Exiguobacterium sp category (Exiguobacterium sp.).
Further, the black smelly water harnessing strain comprises the following components in parts by weight component: 13~28 parts of acinetobacter,
4~17 parts of bacillus, 2~13 parts of pseudomonas, anaerobism Bacillus adhaerens belong to 1~8 part, 3~14 parts of alcaligenes, acid
2~10 parts of jujube Pseudomonas and Exiguobacterium sp belong to 9~26 parts.
Further, the drying protectant is at least one of plant ash, Green maize straw powder and diatomite.It is logical
Cross using drying protectant, can protecting effect in the drying process to black and odorous water processing microorganism it is good, microorganism is deposited
Motility rate is high, convenient for storage and transports, and the low environmental sound of cost of drying protectant.
Further, the colony induction signaling molecule compound includes following components: C4-HSL, C6-HSL, C12-
HSL, 3OC8-HSL, 3OC12-HSL and AI-2.The AI-2 colony induction signaling molecule is produced by Ha Weishi vibrios BB170
Gained, using the centrifugation supernatant bacterium solution of Ha Weishi vibrios BB170 culture bacterium solution.The molecular structural formula of the AI-2 is as follows:
The present invention is by by C4-HSL (N-Butanoyl-L-homoserine lactone, N- butyryl-L- homoserine
Lactone), C6-HSL (N-hexanoyl-L-Homoserine lactone, N- hexanoyl-L- homoserine lactone), C12-HSL
(N-dodecanoyl-L-homoserine lactone, N- dodecane acyl group-L- homoserine lactone), 3OC8-HSL (N-
3-oxo-hexanoyl-homoserine lactone, N-3- oxo-hexanoyl-homoserine lactone), 3OC12-HSL (N-3-
Oxo-dodecanoyl homoserine lactone, N-3- oxo-dodecanoyl-homoserine lactone) and AI-2 match
It closes, can promote in the microbial species of black smelly water harnessing microbial inoculum and the signal of inter-species is transmitted and exchanged, and the row of regulating and controlling microbial
For.By rationally adding this few class signaling molecule, the shape of biomembrane of the microorganism in black and odorous water bottom sludge is greatly reinforced
At higher anti-current-rush ability being provided simultaneously with, to realize so that effectively enhancement microbiological is fixed in black and odorous water
The improvement permanently effective to black and odorous water.
Another object of the present invention is achieved through the following technical solutions:
(1) black smelly water harnessing strain is activated, is inoculated into LB culture medium, it is small in 36~38 DEG C of culture 23-25
When, gained bacterium solution is centrifuged, supernatant is pipetted, remaining bacterium mud is collected, is dried, black smelly water harnessing bacterium mud is made.
(2) Ha Weishi vibrios BB170 is inoculated into LB culture medium, 36~38 DEG C culture 23-25 hours, to gained
Culture bacterium solution be centrifuged, collect supernatant, be made culture bacterium solution Ha Weishi vibrios BB170 centrifugation supernatant bacterium solution；
(3) by C4-HSL, C6-HSL, C12-HSL, 3OC8-HSL, 3OC12-HSL are dissolved in the water, and are then added to step
Suddenly in (1) black smelly water harnessing bacterium mud, the centrifugation supernatant bacterium solution of Ha Weishi vibrios BB170 is then added, is stirred, drying is added
Protective agent, stirring, resulting mixture is dried, tabletting, black smelly water harnessing microbial inoculum is made.
Further, in the step (2), the centrifugation supernatant bacterium solution OD of Ha Weishi vibrios BB170600Value for 0.8~
3.0；In the resulting black smelly water harnessing microbial inoculum of step (3), the additive amount of the centrifugation supernatant bacterium solution of Ha Weishi vibrios BB170
For 0.1~5mL/g.
Further, in the resulting black smelly water harnessing microbial inoculum of the step (3), the additive amount of C4-HSL is 0.01~
The additive amount of 0.25mg/g, C6-HSL are 0.01~0.1mg/g, the additive amount of C12-HSL is 0.01~0.25mg/g, 3OC8-
The additive amount of HSL is 0.01~0.5mg/g, the additive amount of 3OC12~HSL is 0.01~0.25mg/g.
The present invention is aided with a variety of corresponding micropopulations induction letters by using based on black smelly water harnessing bacterium mud
Number molecule not only has to the efficient processing capacity of black and odorous water, and by the additional amount of each signaling molecule of reasonable disposition and matches
Than greatly promoting black and odorous water efficient process microorganism and quickly forming biomembrane in black and odorous water bottom sludge, and make biology
Film has higher anti-current-rush ability, can be realized the improvement permanently effective to black and odorous water, is simple and efficient and treatment cost
It is low.
The present invention also provides the application of the black smelly water harnessing microbial inoculum based on quorum sensing, the black smelly water harnessing microbial inoculum is used
In administering black smelly water, the black smelly water harnessing microbial inoculum is 20-40mg/L in the dosage of black smelly water.The black smelly water harnessing bacterium
Agent is simple and convenient efficient in practical applications, by above-mentioned dosage, not only has and realizes efficiently and for a long time to black and odorous water
Processing capacity, and do not need using special device, treatment cost is low.
Beneficial effects of the present invention: black and odorous water of the invention administers microbial inoculum with black and odorous water efficient process microorganism and is
It is main, while being aided with a variety of corresponding micropopulation induction signal molecules, black and odorous water, which administers microbial inoculum, not only to be had to black smelly water
The efficient processing capacity of body, while biomembrane is quickly formed in black and odorous water bottom sludge, have higher anti-current-rush energy
Power can be realized the improvement permanently effective to black and odorous water.Black and odorous water efficient process micro-organisms process cost is low, if
Standby technological operation is simple, it can be achieved that industrial scale produces, and has significant environment and economic benefit, needs not rely on any
Special device, it is simple and convenient in practical applications, the improvement of various black and odorous waters can be widely used in.
Specific embodiment
For the ease of the understanding of those skilled in the art, below with reference to embodiment, the present invention is further illustrated, real
The content that the mode of applying refers to not is limitation of the invention.
Embodiment 1
In the present embodiment, a kind of preparation method of the black smelly water harnessing microbial inoculum based on quorum sensing, comprising the following steps:
(1) black smelly water harnessing strain is activated, is inoculated into LB culture medium, the LB culture medium includes NaCl
10g/L, yeast powder 5g/L and peptone 10g/L are cultivated 24 hours at 37 DEG C, are centrifuged to gained bacterium solution, pipette supernatant
Liquid is collected remaining bacterium mud, is dried, and the drying temperature is 30 DEG C, pressure 0.2MPa, and black smelly water harnessing is made in dry 9h
Bacterium mud；The black smelly water harnessing strain comprises the following components in parts by weight component: 28 parts of acinetobacter, 17 parts of bacillus, vacation
12 parts of zygosaccharomyces, anaerobism Bacillus adhaerens belong to 12 parts, 8 parts of alcaligenes, 13 parts of wild jujube Pseudomonas and Exiguobacterium sp belong to 10 parts；
(2) Ha Weishi vibrios BB170 is inoculated into LB culture medium, is cultivated 24 hours at 37 DEG C, to resulting culture bacterium
Liquid is centrifuged, and supernatant is collected, and the centrifugation supernatant bacterium solution of Ha Weishi vibrios BB170 is made；
(3) by 2.5mg C4-HSL, 1mg C6-HSL, 2.5mg C12-HSL, 5mg 3OC8-HSL, 2.5mg 3OC12-
In HSL dissolution in distilled water, it is then added in the black smelly water harnessing bacterium mud of 5g, then adds 20mL Ha Weishi vibrios BB170
Centrifugation supernatant bacterium solution, stir 40 minutes, be added 5g hay ash, stir 40 minutes, gained microbial inoculum is dried, tabletting, make
Obtain black smelly water harnessing microbial inoculum.
Embodiment 2
In the present embodiment, a kind of preparation method of the black smelly water harnessing microbial inoculum based on quorum sensing, comprising the following steps:
(1) black smelly water harnessing strain is activated, is inoculated into LB culture medium, the LB culture medium includes NaCl
10g/L, yeast powder 5g/L and peptone 10g/L are cultivated 24 hours at 37 DEG C, are centrifuged to gained bacterium solution, pipette supernatant
Liquid is collected remaining bacterium mud, is dried, and the drying temperature is 35 DEG C, pressure 0.1MPa, and black smelly water harnessing is made in dry 6h
Bacterium mud；The black smelly water harnessing strain comprises the following components in parts by weight component: 23 parts of acinetobacter, 16 parts of bacillus, vacation
13 parts of zygosaccharomyces, anaerobism Bacillus adhaerens belong to 1 part, 14 parts of alcaligenes, 8 parts of wild jujube Pseudomonas and Exiguobacterium sp belong to 2 parts；
(2) Ha Weishi vibrios BB170 is inoculated into LB culture medium, is cultivated 25 hours at 37 DEG C, to resulting culture bacterium
Liquid is centrifuged, and supernatant is collected, and the centrifugation supernatant bacterium solution of Ha Weishi vibrios BB170 is made；
(3) 1mg C4-HSL, 1mg C6-HSL, 1mg C12-HSL, 1mg 3OC8-HSL, 1mg 3OC12-HSL are dissolved
In middle distilled water, it is then added in the black smelly water harnessing bacterium mud of 5g, then adds in the centrifugation of 1mL Ha Weishi vibrios BB170
Clear bacterium solution, stirring after sixty minutes, are added 2.5g Green maize straw powder and 2.5g diatomite, stir 60 minutes, by gained microbial inoculum into
Black smelly water harnessing microbial inoculum is made in row drying, tabletting.
Embodiment 3
In the present embodiment, a kind of preparation method of the black smelly water harnessing microbial inoculum based on quorum sensing, comprising the following steps:
(1) black smelly water harnessing strain is activated, is inoculated into LB culture medium, the LB culture medium includes NaCl
10g/L, yeast powder 5g/L and peptone 10g/L are cultivated 24 hours at 37 DEG C, are centrifuged to gained bacterium solution, pipette supernatant
Liquid is collected remaining bacterium mud, is dried, and the drying temperature is 25 DEG C, pressure 0.3MPa, and dry 12h is made black smelly water and controls
Manage bacterium mud；The black smelly water harnessing strain composition is same as Example 1；
(2) Ha Weishi vibrios BB170 is inoculated into LB culture medium, is cultivated 24 hours at 37 DEG C, to resulting culture bacterium
Liquid is centrifuged, and supernatant is collected, and the centrifugation supernatant bacterium solution of Ha Weishi vibrios BB170 is made；
(3) by 0.1mg C4-HSL, 0.1mg C6-HSL, 0.1mg C12-HSL, 0.1mg 3OC8-HSL, 0.1mg
In 3OC12-HSL dissolution in distilled water, it is then added in the black smelly water harnessing bacterium mud of 5g, then adds 50mL Ha Weishi arc
The centrifugation supernatant bacterium solution of bacterium BB170 stirs 40 minutes, and 2.5g hay ash and 2.5g diatomite stir 30 minutes, by gained bacterium
Agent is dried, tabletting, and black smelly water harnessing microbial inoculum is made.
Comparative example 1
In this comparative example, a kind of preparation method of the black smelly water harnessing microbial inoculum based on quorum sensing, comprising the following steps:
(1) black smelly water harnessing strain is activated, is inoculated into LB culture medium, the LB culture medium includes NaCl
10g/L, yeast powder 5g/L and peptone 10g/L are cultivated 24 hours at 37 DEG C, are centrifuged to gained bacterium solution, pipette supernatant
Liquid collects remaining bacterium mud, is dried, black smelly water harnessing bacterium mud is made；The black smelly water harnessing strain composition and embodiment 1
It is identical；
(2) 5g bacterium mud is weighed, 5g hay ash is added, stirs 40 minutes, gained microbial inoculum is dried, tabletting, is made black
Smelly water harnessing microbial inoculum.
Comparative example 2
In this comparative example, a kind of preparation method of the black smelly water harnessing microbial inoculum based on quorum sensing, comprising the following steps:
(1) black smelly water harnessing strain is activated, is inoculated into LB culture medium, the LB culture medium includes NaCl
10g/L, yeast powder 5g/L and peptone 10g/L are cultivated 24 hours at 37 DEG C, are centrifuged to gained bacterium solution, pipette supernatant
Liquid collects remaining bacterium mud, is dried, black smelly water harnessing bacterium mud is made；The black smelly water harnessing strain composition and embodiment 1
It is identical；
(2) in distilled water, it will be then added in the black smelly water harnessing bacterium mud of 5g in 5mg C4-HSL dissolution, stir 40 points
Clock is added 5g hay ash, stirs 40 minutes, gained microbial inoculum is dried, tabletting, black smelly water harnessing microbial inoculum is made.
Comparative example 3
This comparative example and comparative example 2 the difference is that: signaling molecule is replaced into 5mg C6-HSL.
Comparative example 4
This comparative example and comparative example 2 the difference is that: signaling molecule is replaced into 5mg C12-HSL.
Comparative example 5
This comparative example and comparative example 2 the difference is that: signaling molecule is replaced into 5mg 3OC8-HSL.
Comparative example 6
This comparative example and comparative example 2 the difference is that: signaling molecule is replaced into 5mg 3OC12-HSL.
Comparative example 7
The preparation method of the microbial bacterial agent of this comparative example, comprising the following steps:
(1) black smelly water harnessing strain is activated, is inoculated into LB culture medium, the LB culture medium includes NaCl
10g/L, yeast powder 5g/L and peptone 10g/L are cultivated 24 hours at 37 DEG C, are centrifuged to gained bacterium solution, pipette supernatant
Liquid collects remaining bacterium mud, is dried, black smelly water harnessing bacterium mud is made；The black smelly water harnessing strain composition and embodiment 1
It is identical,
(2) the centrifugation supernatant bacterium solution that 50mL Ha Weishi vibrios BB170 is added is added in 5g bacterium mud, then stirs 40
Minute, 5g hay ash is added, stirs 40 minutes, gained microbial inoculum is dried, tabletting, black smelly water harnessing microbial inoculum is made.
Comparative example 8
This comparative example difference from example 1 is that: the black smelly water harnessing strain of embodiment 1 is replaced with into control
Microorganism fungus kind, the control microorganism fungus kind comprise the following components in parts by weight: 12 parts of lactobacillus, 9 parts of brevibacterium,
6 parts of vibrio, 9 parts of acinetobacter, 4 parts of bacillus, 4 parts of pseudomonas, anaerobism Bacillus adhaerens belong to 8 parts, wild jujube Pseudomonas
3 parts and Exiguobacterium sp belong to 1 part.
The measurement of biofilm thickness is carried out to embodiment 1-3 and comparative example 1-8: being taken after boiling on a small quantity and is restored to room temperature
Black and odorous water overlying water, add microbial inoculum obtained (1L sewage adds microbial inoculum 30mg), then filtered, be placed in qualitative filter paper
Biofilm formation measures reactor, biofilm thickness is measured after 24 hours, experimental result is as shown in table 1:
Experimental result of the table 1 embodiment 1-3 and comparative example 1-8 to Biofilm
The measurement of COD removal rate and ammonia nitrogen removal frank is carried out to embodiment 1-3 and comparative example 1,11-13: being taken first a small amount of
Black and odorous water overlying water that is rear and being restored to room temperature is boiled, adding the resulting microbial inoculum of abovementioned steps, (1L sewage adds microbial inoculum
30mg), it vibrates 2 days, then acquires water sample, and carry out high speed centrifugation processing, supernatant is taken to be measured, comparison culture front and back
COD and ammonia nitrogen degradation situation, experimental result are as shown in table 2:
The measurement result of table 2 COD removal rate and ammonia nitrogen removal frank
By table 1-2 it is found that the embodiment of the present invention 1-3 passes through black smelly water harnessing bacterium mud, drying protectant and group
Induction signal molecule compound cooperates, and black smelly water harnessing microbial inoculum obtained has excellent COD removal rate and ammonia nitrogen removal
Rate, and biomembrane can be quickly formed, have higher anti-current-rush ability, can be realized permanently effective to black and odorous water control
Reason.
Above-described embodiment is the preferable implementation of the present invention, and in addition to this, the present invention can be realized with other way,
Without departing from the inventive concept of the premise it is any obviously replace it is within the scope of the present invention.
Claims (10)
1. a kind of black smelly water harnessing microbial inoculum based on quorum sensing, it is characterised in that: be made of the component of following parts by weight: black smelly
30~50 parts of water harnessing bacterium mud, 50~70 parts of drying protectant, 0.001~0.1 part of colony induction signaling molecule compound.
2. the black smelly water harnessing microbial inoculum according to claim 1 based on quorum sensing, it is characterised in that: the black smelly water is controlled
The preparation method of bacterium mud is managed the following steps are included: black and odorous water improvement strain is activated, is inoculated into LB culture medium, 36
~38 DEG C culture 23-25 hours, gained bacterium solution is centrifuged, pipettes supernatant, remaining bacterium mud is collected, is dried, be made
The black smelly water harnessing bacterium mud of stand-by bacterium.
3. the black smelly water harnessing microbial inoculum according to claim 2 based on quorum sensing, it is characterised in that: the black smelly water is controlled
Manage strain include following components: acinetobacter, bacillus, pseudomonas, anaerobism Bacillus adhaerens category, alcaligenes,
Wild jujube Pseudomonas and Exiguobacterium sp category.
4. the black smelly water harnessing microbial inoculum according to claim 2 based on quorum sensing, it is characterised in that: the black smelly water is controlled
Reason strain comprises the following components in parts by weight component: 13~28 parts of acinetobacter, 4~17 parts of bacillus, pseudomonas 2~
13 parts, anaerobism Bacillus adhaerens belong to 1~8 part, 3~14 parts of alcaligenes, 2~10 parts of wild jujube Pseudomonas and Exiguobacterium sp belong to 9~26
Part.
5. the black smelly water harnessing microbial inoculum according to claim 1 based on quorum sensing, it is characterised in that: the dry-run protection
Agent is at least one of plant ash, Green maize straw powder and diatomite.
6. the black smelly water harnessing microbial inoculum according to claim 1 based on quorum sensing, it is characterised in that: the quorum sensing
Signaling molecule compound includes following components: C4-HSL, C6-HSL, C12-HSL, 3OC8-HSL, 3OC12-HSL and AI-2.
7. the preparation method of the black smelly water harnessing microbial inoculum according to claim 1-6 based on quorum sensing, special
Sign is: the following steps are included:
(1) black smelly water harnessing strain is activated, is inoculated into LB culture medium, 36~38 DEG C culture 23-25 hours, to institute
It obtains bacterium solution to be centrifuged, pipettes supernatant, collect remaining bacterium mud, be dried, black smelly water harnessing bacterium mud is made；
(2) Ha Weishi vibrios BB170 is inoculated into LB culture medium, 36~38 DEG C culture 23-25 hours, to resulting culture
Bacterium solution is centrifuged, and supernatant is collected, and the centrifugation supernatant bacterium solution of Ha Weishi vibrios BB170 is made；
(3) by C4-HSL, C6-HSL, C12-HSL, 3OC8-HSL, 3OC12-HSL are dissolved in the water, and are then added to step (1)
In black smelly water harnessing bacterium mud, the centrifugation supernatant bacterium solution of Ha Weishi vibrios BB170 is then added, is stirred, drying protectant is added,
Stirring, resulting microbial inoculum mixture is dried, tabletting, black smelly water harnessing microbial inoculum is made.
8. the preparation method of the black smelly water harnessing microbial inoculum according to claim 7 based on quorum sensing, it is characterised in that: institute
It states in step (2), the centrifugation supernatant bacterium solution OD of Ha Weishi vibrios BB170600Value is 0.8~3.0；The step (3) is resulting black
In smelly water harnessing microbial inoculum, the additive amount of the centrifugation supernatant bacterium solution of Ha Weishi vibrios BB170 is 0.1~5mL/g.
9. the preparation method of the black smelly water harnessing microbial inoculum according to claim 7 based on quorum sensing, it is characterised in that: institute
It states in the resulting black smelly water harnessing microbial inoculum of step (3), the additive amount of C4-HSL is the additive amount of 0.01~0.25mg/g, C6-HSL
Additive amount for 0.01~0.1mg/g, C12-HSL is 0.01~0.25mg/g, the additive amount of 3OC8-HSL be 0.01~
0.5mg/g, 3OC12~HSL additive amount are 0.01~0.25mg/g.
10. the application of described in any item black smelly water harnessing microbial inoculums based on quorum sensing, feature described in claim 1-6
Be: the black smelly water harnessing microbial inoculum is 20- in the dosage of black smelly water for administering black smelly water, the black smelly water harnessing microbial inoculum
40mg/L。
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