CN107434305A - A kind of water purification agent of rich defect carbon carrier fixation of microbe and preparation method thereof - Google Patents
A kind of water purification agent of rich defect carbon carrier fixation of microbe and preparation method thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F2003/001—Biological treatment of water, waste water, or sewage using granular carriers or supports for the microorganisms
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Abstract
The invention belongs to microbial water-purifying agent technical field, and in particular to a kind of water purification agent of rich defect carbon carrier fixation of microbe and preparation method thereof.Rich defect carbon carrier is placed in culture medium, while compound strain is seeded to culture medium, is co-cultured through vibration and water purification agent is made.Because the carbon support of the water purification agent has oxygen-containing functional group, the hydrophobic sex chromosome mosaicism of general high-area carbon is overcome, and carbon support is rich in defect and secondary pore structure, provides more avtive spots for adsorption reaction, improves the adsorption efficiency of pollutant；Rich defect carbon carrier containing hydrophilic radical is advantageous to absorption of the carbon carrier to microorganism with microbial co culture to be fixed; the organic matter that adsorbs simultaneously in carrier carbon and pollutant is efficiently utilized by microorganism respectively and Synergistic degradation; so the water purification agent have the advantages that it is hydrophilic but the characteristics of be easy to precipitation, carbon adsorption and biodegradable double purified act on, be highly suitable for cultivating the scale processing of anaerobism sewage.
Description
Technical field
The invention belongs to microbial water-purifying agent technical field, and in particular to a kind of rich defect carbon carrier fixation of microbe it is net
Aqua and preparation method thereof.
Technical background
In animal husbandry large-scale development, unified management, cost is reduced, improve economic benefit, but largely concentrate
Heavy environmental pollution caused by sewage discharge, it is badly in need of administering.Mainly there is recycling to aquaculture wastewater processing mode both at home and abroad
With the major class of qualified discharge two, 3 kinds of returning to the field pattern, Natural treatment mode and industrial treatment patterns are summarized as, they respectively have excellent lack
Point.The purpose of China aquaculture wastewater processing is control, reduce it caused by harm, carry out comprehensive utilization, finally realize minimizing,
Recycling, innoxious and qualified discharge target, reduce the influence to crop soil quality and crop quality, reduce to water body,
The potential pollution risk of soil environment and climatic environment.
Recent study persons are directed to the nature and characteristic of agricultural environment pollution thing, to the processing method and technique of aquaculture wastewater
All carry out compared with systematic research, be utilized respectively chemical method and biological method to nitrogen, phosphorus, heavy metal ion and organic matter point
Son adsorbed, is separated and biology/chemical degradation, and single processing mode realizes minimizing and recycling to a certain extent
Target, but it is extremely difficult to complete innoxious and discharging standards.In research process, activated carbon is often used as sewage pollution
The adsorbent of thing, it can take on a new look for the colourity and turbidity of sewage, simultaneously containing macropore, mesopore and microcellular structure, therefore
Suction-operated can also occur for small molecule and heavy metal ion, can also be pressed into column little particle as biologic water cleaner
Carrier is used for the purified treatment of fowl poultry kind cultivation anaerobism biogas slurry, and single bacterial strain is fixed on the bacterium carbon water purification agent of carbon carrier formation,
Its wastewater purifying efficiency is often not as the bacterium carbon ideal purification effect of compound strain formation.Publication No. is the A's of CN 104261570
Patent discloses a kind of livestock and poultry cultivation anaerobism purifying agent for polluted water, is made up of complex microorganism bacterial strain with absorbent charcoal carrier, the work
Property high-area carbon be ature of coal column-shaped active carbon, be by solid cylindrical activity made of sizing with the coal mass active carbon of 2-10 mesh
Charcoal.Coal mass active carbon used and commercially available carbon material（Activated carbon AC, CNT CNT, graphite GC etc.）Have many
Advantage, such as porosity are high, bigger than surface, stability is good, nontoxic pollution-free-environment-friendly；But its surface functional group is less, no
Containing hydrophilic radical（-COOH、-OH）, therefore cleanser dispersiveness in water is poor, hinders and is fully connect with dirty water pollutant
Touch, and be difficult that electrostatic interaction occurs with the ammonia nitrogen substances of hydrogen bonds and lone electron pair, it is impossible to play carbon-based supports completely
Suction-operated to pollutant, therefore surface parent/hydrophobic regulation and control to carbon material are to seem very necessary.It is currently used
Method is to use concentrated acid（Dense HNO3, dense H2SO4）Activate inert carbon surface and surface char combustion is formed oxygen-containing functional group（-
COOH、-OH、-C=O）, while have the nitrogen oxides and oxysulfide of pollution, cause secondary pollution；And utilize 600-
Alkali under 900 DEG C or so the conditions of high temperature reacts in inert gas environment with carbon, etches secondary pore structure and surface
Avtive spot, these method severe reaction conditions and requires reaction vessel acid and alkali-resistance and high temperature, in practical operation and application process
In many deficiencies all be present.
The content of the invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide a kind of the net of rich defect carbon carrier fixation of microbe
Aqua and preparation method thereof.Carbon carrier used is rich in hydrophilic radical, cleanser and water is had more preferable intermiscibility, overcomes
The hydrophobic sex chromosome mosaicism of general high-area carbon, and the absorption for being more beneficial for microbial strains is fixed, the contact between pollutant
Property it is more preferable, improve the adsorption efficiency of pollutant, and because being rich in secondary pore structure and surface-active site, add to pollution
The absorption of thing.There is the water purification agent of the rich defect carbon carrier fixation of microbe carbon adsorption to be acted on biodegradable double purified,
Realize simultaneously microorganism to organic matter recycle and collaboration efficient degradation to pollutant.
For achieving the above object, the present invention adopts the following technical scheme that：
A kind of water purification agent of rich defect carbon carrier fixation of microbe：Rich defect carbon carrier is placed in Luria-Bertani culture mediums
In, while compound strain is seeded to Luria-Bertani culture mediums, co-cultured, after drying through vibration, water purification agent is made；Institute
The preparation method for the rich defect carbon carrier stated comprises the following steps：
（1）90g NaOH and KOH mixed base are placed in 200 ml reactor inner bags, is placed at 165 DEG C and melts 2h, obtain clear
Clear melting alkali lye；
（2）Using glucose and urea as raw material, add in fining molten alkali lye and react 12 hours, generate rich defect powder carbon forerunner
Body material；
（3）After water, absolute ethyl alcohol washing, powder carbon matrix precursor is obtained in 80 DEG C of dry 12h successively for carbon matrix precursor material；
（4）Powder carbon matrix precursor is placed in 300 DEG C of Muffle furnaces and calcines 2h, obtains porous rich defect carbon powder material（Total hole volume
About 0.05 ~ 0.06 cm3/g）；
（5）Porous rich defect carbon powder material is scattered in ethanol, polyethylene glycol is then instilled in dispersion liquid, is sufficiently stirred
It is compressing after forming jelly, the carbon carrier of microbial water-purifying agent is made.
The formula of described Luria-Bertani culture mediums is：Tryptone 10g, yeast extract 5g, NaCl 10g,
Distilled water 1000 mL, pH 7.0.
Step（1）Described in mixed base in NaOH and KOH mass ratio be 46.5:43.5.
A kind of method for the water purification agent for preparing rich defect carbon carrier fixation of microbe as described above：By 10g richness defect carbon
Carrier is added in 50-150 ml Luria-Bertani culture mediums, and by bacillus licheniformisBacillus licheniformis, Rhodopseudomonas palustrisRhodop seudanonas palustris, chlorellaChlorella vulgaris, streptococcusStreptococcus, thiobacillus thiooxidantThiobacillus ThiooxidantIt is 4 in mass ratio:5:
1:4:1 is seeded in Luria-Bertani culture mediums, and compound strain inoculum concentration is 1-2%, is vibrated under the conditions of 4 DEG C, 150 rpm
48~72h is co-cultured, carbon carrier is finally pulled out and is placed in 4 DEG C of insulating box and dry, that is, water purification agent is made；It is effectively living on carbon carrier
Bacterium number is 7 × 108~6 × 109 CFU/g, bacterium carbon Percentage bound reach 97.8%.
During rich defect carbon carrier and microbial co culture, hydrophilic Gram-negative bacteria（Thiobacillus thiooxidant/natural pond
Damp red pseudomonas）Show electropositivity because cell surface composition is mainly liopopolysaccharides, can be adsorbed by electrostatic interaction solid
Due on the carbon carrier of negatively charged group；And gram-positive bacteria（Chlorella/bacillus/streptococcus）Cell membrane by one layer
The peptide glycan and LTA composition of thick and fine and close 5 glycine crosslinking, the elecrtonegativity on surface make it that hydrophobicity be presented, cultivated
Growth early stage can be spread using its hydrophobicity in nutrient solution, multiple resources be obtained to meet propagation demand, in growth course
Microorganism hydrophobicity, which declines, makes it freely to be swum in water body from environment-stress, survival ability is improved, into Later growth
Microorganism hydrophily further increases, and is easy to absorption to be fixed on the hydrophily carbon carrier of negatively charged group, it can be seen that, containing parent
The absorption that the carbon carrier of water-based functional group is advantageous to microbial strains is fixed.
A kind of water purification agent of rich defect carbon carrier fixation of microbe as described above is stored under the conditions of 4 DEG C, using it is preceding
8-12 hours are placed under normal temperature and recover application after bioactivity in terms of processing cultivation anaerobism sewage.
The strain used in above-mentioned raw materials can identify that separation method is convenient by those skilled in the art by conventional bacterial classification
Ground is isolated from nature, or the strain by commercial channel open purchase.The strain that the present invention uses can be micro- from China
The purchase of the suppliers such as biological inoculum net-Beijing North Na Chuanlian Bioteknologisk Institut obtains.
Above-mentioned strain is according to existing conventional method culture：
The culture medium of I bacillus licheniformis：Tryptone 5g, yeast extract 15g, K2HPO43g, glucose 2g, distilled water 1000
ML, pH 7.5；
The culture medium of II Rhodopseudomonas palustris：NH4Cl 1.0g, K2HPO40.5g, NaHCO33.0g, yeast extract 2.0g, steam
Distilled water 1000 mL, pH 7.2；
The conventional culture formula of liquid of III chlorella：NH4NO350-100 mg, K2HPO45 mg, FeC6H5O70.1-0.5 mg, sea
The mL of water 1000；
IV streptococcic culture medium：Powdered beef 10.0g, tryptone 20.g, glucose 2.0g, NaHCO32.0g, NaCl
2.0g, Na2HPO40.4g, the mL of distilled water 1000, pH value 7.8；
The culture medium of V thiobacillus thiooxidant：Tryptone 5.0g, KH2PO41.0g, MgSO4·7H2O 0.5g, glucose 10g,
The mL of distilled water 1000.
The beneficial effects of the present invention are：
1）Microbial water-purifying agent of the present invention is thrown in cultivation anaerobism sewage, water purification agent can utilize big in microbial degradation sewage
Molecular organic, most pollutant in sewage can be removed, reach the purpose of purification sewage；
2）It is raw material because the carbon carrier of microbial water-purifying agent employs organic compound, the oxygen-containing function of carbon support of preparation
Group（- OH ,-COOH etc.）Abundant, with the Hydrogen Proton in water/hydroxide ion electrostatic interaction occurs for these charging property groups,
Carbon carrier and water is had more preferable intermiscibility, overcome the hydrophobic sex chromosome mosaicism of general high-area carbon, it is more preferable with pollutant contact；
With the lone pair electrons of nitrogen phosphate and sulfur electrostatic interaction can occur for the charging property group on carbon support simultaneously, improve to pollutant
Adsorption efficiency；Secondly, 300 DEG C of calcination process also improve the purity and porosity of carbon dust, and the solid base environment of melting makes
Carbon material obtains surface defect and secondary pore structure, and more avtive spots are provided for adsorption reaction, adds to pollution
The absorption of thing；
3）By by hydrophily carbon carrier and microbial co culture, on obtained water purification agent carbon carrier living bacteria count be 7 ×
108~6 × 109 CFU/gCFU/g, bacterium carbon Percentage bound reach 97.8%, improve absorption fixed efficiency of the carbon carrier to microorganism；
4）The water purification agent of the rich defect carbon carrier fixation of microbe will be adsorbed due to the microorganism that is colonized on activated carbon on carbon
Organic matter etc. is used as nutriment efficient degradation, has both purified water quality, again can be using the product of own metabolism as utilizing each other
Nutritional substrate, microbial activity is improved, promote microorganism species to pollutant Synergistic degradation, therefore with carbon adsorption and biodegradation
Double purified effect, it is hydrophilic but the characteristics of be easy to precipitation, clean-up effect it is stable and the advantages that be easily handled, be highly suitable for supporting
Grow the scale processing of anaerobism sewage.
Embodiment
For the further open rather than limitation present invention, below in conjunction with example, the present invention is described in further detail.
A kind of preparation method of the water purification agent of rich defect carbon carrier fixation of microbe, is concretely comprised the following steps：
1st, the preparation of carbon carrier of the surface containing hydrophilic functional group and defect
1）By NaOH+KOH=90g（NaOH:KOH=46.5:43.5 mass ratio）It is placed in 200 ml reactor inner bags, is placed in 165
2h is melted at DEG C, obtains fining molten alkali lye；
2）Using glucose and urea as raw material, add in fining molten alkali lye and react 12 hours, generate rich defect powder carbon forerunner
Body materials A；
3）It is about 7 that above-mentioned carbon matrix precursor A is washed with water into washing lotion pH value, removes water-solubility impurity, is then centrifuged for separating
Layer precipitation；Lower sediment is washed with absolute ethyl alcohol again, removes organic impurities not soluble in water, and the sediment finally centrifuged is in 80 DEG C
It is dried overnight, obtains rich defect powder carbon precursor samples A；
4）Carbon matrix precursor sample A is placed in 300 DEG C of Muffle furnaces and calcines 2h, obtains porous rich defect carbon dust B（Total hole volume is about
0.05~0.06 cm3/g）；
5）0.5-1.0 grams of porous rich defect carbon dust B is placed in agate mortar, adds 2-4 ml ethanol, is stirred 30 minutes
Carbon dust is dispersed in ethanol, add 1-2 ml polyethylene glycol（PEG400）, being sufficiently stirred makes powder carbon form glue
It is put into after shape thing in the compressing tablet groove of tablet press machine（Cylindrical type, tablet type）Or in the mould of compression resistance, it is placed in 80 DEG C of environment
Dry 24 hours, it is finally compressing under 10-15MPa pressure, the carbon carrier of microbial water-purifying agent is made.
2nd, 10g richness defect carbon carriers are added in 50-150 ml Luria-Bertani culture mediums, and by lichens bud
Spore bacillusBacillus licheniformis, Rhodopseudomonas palustrisRhodop seudanonas palustris, chlorellaChlorella vulgaris, streptococcusStreptococcus, thiobacillus thiooxidantThiobacillus ThiooxidantBy matter
Amount is than being 4:5:1:4:1 is seeded in Luria-Bertani culture mediums, and compound strain inoculum concentration is 1-2 %, 4 DEG C, 150
Vibration co-cultures 48~72h under the conditions of rpm, finally pulls carbon carrier out and is placed in 4 DEG C of insulating box and dries, that is, water purification agent is made；
Living bacteria count is 7 × 10 on carbon carrier8~6 × 109 CFU/g, bacterium carbon Percentage bound reach 97.8%；What is preserved under the conditions of 4 DEG C is net
Aqua places 8-12 hours under normal temperature and recovers bioactivity and can directly be invested in sewage using preceding.
Embodiment 1
A kind of preparation method of the water purification agent of rich defect carbon carrier fixation of microbe, is concretely comprised the following steps：
（1）The preparation of carbon carrier of the surface containing hydrophilic functional group and defect
1）By NaOH+KOH=90g（NaOH:KOH=46.5:43.5 mass ratio）It is placed in 200 ml reactor inner bags, is placed in 165
2h is melted at DEG C, obtains fining molten alkali lye；
2）Using glucose and urea as raw material, add in fining molten alkali lye and react 12 hours, generate rich defect powder carbon forerunner
Body materials A；
3）It is about 7 that above-mentioned carbon matrix precursor A is washed with water into washing lotion pH value, removes water-solubility impurity, is then centrifuged for separating
Layer precipitation；Lower sediment is washed with absolute ethyl alcohol again, removes organic impurities not soluble in water, and the sediment finally centrifuged is in 80 DEG C
It is dried overnight, obtains rich defect powder carbon precursor samples A；
4）Carbon matrix precursor A is placed in 300 DEG C of Muffle furnaces and calcines 2h, obtains porous rich defect carbon powder material B；
5）0.8 gram of more hole defect carbon dust B are placed in agate mortar, add 3 ml ethanol, stirring makes carbon dust in 30 minutes
It is dispersed in ethanol, adds 1.6 ml polyethylene glycol（PEG400）, being sufficiently stirred makes powder carbon be put after forming jelly
Enter into the compressing tablet groove of tablet press machine（Cylindrical type, tablet type）Or in the mould of compression resistance, it is small that dry 24 are placed in 80 DEG C of environment
When, it is finally compressing under 15 MPa pressure, the carbon carrier of microbial water-purifying agent is made.
（2）10g richness defect carbon carriers are added in 150 ml Luria-Bertani culture mediums, and by lichens gemma bar
BacteriumBacillus licheniformis, Rhodopseudomonas palustrisRhodop seudanonas palustris, chlorellaChlorella vulgaris, streptococcusStreptococcus, thiobacillus thiooxidantThiobacillus ThiooxidantBy matter
Amount is than being 4:5:1:4:1 is seeded in Luria-Bertani culture mediums, and compound strain inoculum concentration is 1%, in 4 DEG C, 150 rpm bars
Vibration co-cultures 72h under part, finally pulls carbon carrier out and is placed in 4 DEG C of insulating box and dries, that is, water purification agent is made；Have on carbon carrier
It is 6 × 10 to imitate viable count9 CFU/g, bacterium carbon Percentage bound reach 97.8%；The water purification agent preserved under the conditions of 4 DEG C is before use in normal temperature
Lower placement, which recovers bioactivity for 10 hours, can directly be invested in sewage.
Embodiment 2
A kind of preparation method of the water purification agent of rich defect carbon carrier fixation of microbe, is concretely comprised the following steps：
（1）The preparation of carbon carrier of the surface containing hydrophilic functional group and defect
1）By NaOH+KOH=90g（NaOH:KOH=46.5:43.5 mass ratio）It is placed in 200 ml reactor inner bags, is placed in 180
2h is melted at DEG C, obtains fining molten alkali lye；
2）Using glucose and urea as raw material, add in fining molten alkali lye and react 10 hours, generate rich defect powder carbon forerunner
Body materials A；
3）It is about 7 that above-mentioned carbon matrix precursor A is washed with water into washing lotion pH value, removes water-solubility impurity, is then centrifuged for separating
Layer precipitation；Lower sediment is washed with absolute ethyl alcohol again, removes organic impurities not soluble in water, and the sediment finally centrifuged is in 80 DEG C
It is dried overnight, obtains rich defect powder carbon precursor samples A；
4）Carbon matrix precursor A is placed in 300 DEG C of Muffle furnaces and calcines 2h, obtains porous rich defect carbon powder material B；
5）0.5 gram of more hole defect carbon dust B are placed in agate mortar, add 2ml ethanol, stirring makes carbon dust equal in 30 minutes
It is even to be scattered in ethanol, add 1.0 ml drop polyethylene glycol（PEG400）, being sufficiently stirred makes powder carbon be put after forming jelly
Enter into the compressing tablet groove of tablet press machine（Cylindrical type, tablet type）Or in the mould of compression resistance, it is small that dry 24 are placed in 80 DEG C of environment
When, it is finally compressing under 10MPa pressure, the carbon carrier of microbial water-purifying agent is made.
（2）10g richness defect carbon carriers are added in 100 ml Luria-Bertani culture mediums, and by lichens gemma bar
BacteriumBacillus licheniformis, Rhodopseudomonas palustrisRhodop seudanonas palustris, chlorellaChlorella vulgaris, streptococcusStreptococcus, thiobacillus thiooxidantThiobacillus ThiooxidantBy matter
Amount is than being 4:5:1:4:1 is seeded in Luria-Bertani culture mediums, and compound strain inoculum concentration is 2%, in 4 DEG C, 150 rpm bars
Vibration co-cultures 60h under part, finally pulls carbon carrier out and is placed in 4 DEG C of insulating box and dries, that is, water purification agent is made；Have on carbon carrier
It is 1 × 10 to imitate viable count9CFU/g, bacterium carbon Percentage bound reach 97.4%；The water purification agent preserved under the conditions of 4 DEG C is before use in normal temperature
Lower placement, which recovers bioactivity for 10 hours, can directly be invested in sewage.
Embodiment 3
A kind of preparation method of the water purification agent of rich defect carbon carrier fixation of microbe, is concretely comprised the following steps：
（1）The preparation of carbon carrier of the surface containing hydrophilic functional group and defect
1）By NaOH+KOH=90g（NaOH:KOH=46.5:43.5 mass ratio）It is placed in 200 ml reactor inner bags, is placed in 200
2h is melted at DEG C, obtains fining molten alkali lye；
2）Using glucose and urea as raw material, add in fining molten alkali lye and react 8 hours, generate rich defect powder carbon forerunner
Body materials A；
3）It is about 7 that above-mentioned carbon matrix precursor A is washed with water into washing lotion pH value, removes water-solubility impurity, is then centrifuged for separating
Layer precipitation；Lower sediment is washed with absolute ethyl alcohol again, removes organic impurities not soluble in water, and the sediment finally centrifuged is in 80 DEG C
It is dried overnight, obtains rich defect powder carbon precursor samples A；
4）Carbon matrix precursor A is placed in 300 DEG C of Muffle furnaces and calcines 2h, obtains porous rich defect carbon material B；
5）1.0 grams of more hole defect carbon dust B are placed in agate mortar, add 4 ml ethanol, stirring makes carbon dust in 30 minutes
It is dispersed in ethanol, adds 2 ml polyethylene glycol（PEG400）, being sufficiently stirred makes powder carbon be put into after forming jelly
Into the compressing tablet groove of tablet press machine（Cylindrical type, tablet type）Or in the mould of compression resistance, be placed in 80 DEG C of environment and dry 24 hours,
It is finally compressing under 15MPa pressure, the carbon carrier of microbial water-purifying agent is made.
（2）10g richness defect carbon carriers are added in 50 ml Luria-Bertani culture mediums, and by lichens gemma bar
BacteriumBacillus licheniformis, Rhodopseudomonas palustrisRhodop seudanonas palustris, chlorellaChlorella vulgaris, streptococcusStreptococcus, thiobacillus thiooxidantThiobacillus ThiooxidantBy matter
Amount is than being 4:5:1:4:1 is seeded in Luria-Bertani culture mediums, and compound strain inoculum concentration is 1%, in 4 DEG C, 150 rpm bars
Vibration co-cultures 48h under part, finally pulls carbon carrier out and is placed in 4 DEG C of insulating box and dries, that is, water purification agent is made；Have on carbon carrier
It is 7 × 10 to imitate viable count8 CFU/g, bacterium carbon Percentage bound reach 97.1%；The water purification agent preserved under the conditions of 4 DEG C is before use in normal temperature
Lower placement, which recovers bioactivity for 10 hours, can directly be invested in sewage.
Comparative example 1
It is essentially identical with embodiment 1-3, the water purification agent of rich defect carbon carrier fixation of microbe is made, differs only in first three step
Suddenly（1）The carbon carrier of middle preparation is different, the column type absorbent charcoal carrier in comparative example 1 to sell on the market（Than surface about 1100
m2/ g, pore volume are 0.75 cm3/g）.
Performance test：
First, absorbent charcoal carrier of different shapes is put into cultivation anaerobism sewage, its adsorption effect is compared.Weigh respectively
Absorbent charcoal carrier variously-shaped 100g（That is particulate coal activated carbon A, spherical coal mass active carbon B, cylindric coal mass active carbon
C, rich defect column carbon D）, put into 10L cultivation anaerobism sewage, using untreated cultivation anaerobism sewage as control, 3.5d
Determine its CODcr, BOD respectively afterwards5, ammonia nitrogen, the technical indicator such as total phosphorus, calculate activated carbon to each index in cultivation anaerobism sewage
Adsorption rate.
Experimental result is shown in Table 1-1.From experimental result as can be seen that cylindric coal mass active carbon is to cultivating anaerobism in former three
The adsorption capacity of sewage is preferable, its COD, BOD5, ammonia nitrogen, the adsorption rate such as total phosphorus be all higher than the above two；Rich defect post in this patent
Shape carbon adsorption effect is then better than A, B, C three, and COD, BOD5, ammonia nitrogen, total phosphorus adsorption rate have more apparent raising, it is seen that surface
Oxygen-containing functional group and surface defect collaboration promote the absorption of pollutant, and clean effect is better than commercially available different shape activated carbon.
Table 1-1 high-area carbons of different shapes are to cultivating anaerobism wastewater treatment efficiency
2nd, five sizes and the identical cultivation anaerobism sewage tank of water quality are taken（10 L）Tested, first is launched in fact
Apply the microbial water-purifying agent of example 1；The microbial water-purifying agent of second dispensing embodiment 2；Micro- life of 3rd dispensing embodiment 3
Thing water purification agent；The bacterium carbon water purification agent of 4th dispensing comparative example 1；5th is not launched any water purification agent, as blank control group.
100 g water purification agents are thrown to cultivation anaerobism sewage tank respectively, after 3.5 days, to all cultivation anaerobism sewage
Tank carries out water-quality determination.The foundation of water quality measurement is GB11607-89 National Standard of the People's Republic of China's fishery water quality marks
Accurate and NY5051-2001 pollution-free food aquiculture fresh water water quality, testing index and data are as shown in following table 1-2, table 1-3.
The table 1-2 action effects of different bacterium carbon cleansers to sewage index concentration after 3.5 days
The table 1-3 removal effects of different bacterium carbon cleansers to pollutant after 3.5 days
As can be seen from the data in the table, embodiment 1-3 every water quality index is superior to comparative example 1, become apparent from better than control
Group.
The foregoing is only a specific embodiment of the invention, but protection scope of the present invention is not limited thereto, any
Those skilled in the art disclosed herein technical scope in, can without the change that creative work is expected or
Replace, should all be included within the scope of the present invention.Therefore, protection scope of the present invention should be limited with claims
Fixed protection domain is defined.
Claims (5)
- A kind of 1. water purification agent of rich defect carbon carrier fixation of microbe, it is characterised in that：Rich defect carbon carrier is placed in Luria- In Bertani culture mediums, while compound strain is seeded to Luria-Bertani culture mediums, co-cultured, after drying through vibration, Water purification agent is made；The preparation method of described rich defect carbon carrier comprises the following steps：（1）90g NaOH and KOH mixed base are placed in 200 ml reactor inner bags, is placed at 165 DEG C and melts 2h, obtain clear Clear melting alkali lye；（2）Using glucose and urea as raw material, add in fining molten alkali lye and react 12 hours, before generating rich defect powder carbon Drive body material；（3）After water, absolute ethyl alcohol washing, powder carbon matrix precursor is obtained in 80 DEG C of dry 12h successively for carbon matrix precursor material；（4）Powder carbon matrix precursor is placed in 300 DEG C of Muffle furnaces and calcines 2h, it is 0.05 ~ 0.06 cm to obtain total hole volume3/ g's is more Hole richness defect carbon powder material；（5）Porous rich defect carbon powder material is scattered in ethanol, polyethylene glycol is then instilled in dispersion liquid, is sufficiently stirred It is compressing after forming jelly, the carbon carrier of microbial water-purifying agent is made.
- 2. the water purification agent of rich defect carbon carrier fixation of microbe according to claim 1, it is characterised in that：Described The formula of Luria-Bertani culture mediums is：Tryptone 10g, yeast extract 5g, NaCl 10g, the mL of distilled water 1000, pH 7.0。
- 3. the water purification agent of rich defect carbon carrier fixation of microbe according to claim 1, it is characterised in that：Step（1）In NaOH and KOH mass ratio is 46.5 in described mixed base:43.5.
- 4. a kind of method for the water purification agent for preparing the rich defect carbon carrier fixation of microbe as described in claim any one of 1-3, It is characterized in that：10g richness defect carbon carriers are added in 50-150 ml Luria-Bertani culture mediums, and by lichens bud Spore bacillusBacillus licheniformis, Rhodopseudomonas palustrisRhodop seudanonas palustris, chlorellaChlorella vulgaris, streptococcusStreptococcus, thiobacillus thiooxidantThiobacillus ThiooxidantBy matter Amount is than being 4:5:1:4:1 is seeded in Luria-Bertani culture mediums, and compound strain inoculum concentration is 1-2 %, 4 DEG C, 150 48~72h of shaken cultivation under the conditions of rpm, finally pull carbon carrier out and be placed in 4 DEG C of insulating box and dry, that is, water purification agent is made；Carbon Living bacteria count is 7 × 10 on carrier8~6 × 109 CFU/g。
- 5. a kind of water purification agent of rich defect carbon carrier fixation of microbe as described in claim any one of 1-3 is detested in processing cultivation Application in terms of oxygen sewage.
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