CN105695443B - Triton X-100 enhanced biological charcoal immobilized microorganism material and its preparation method and application - Google Patents

Triton X-100 enhanced biological charcoal immobilized microorganism material and its preparation method and application Download PDF

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CN105695443B
CN105695443B CN201610132948.1A CN201610132948A CN105695443B CN 105695443 B CN105695443 B CN 105695443B CN 201610132948 A CN201610132948 A CN 201610132948A CN 105695443 B CN105695443 B CN 105695443B
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triton
immobilized microorganism
charcoal
pseudomonas aeruginosa
microorganism material
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CN105695443A (en
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吕黎
李阿南
孔雪莹
钟作浩
刘欣悦
李青
濮妍琦
何闪英
杨春平
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Zhejiang Gongshang University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/38—Organic compounds containing nitrogen

Abstract

The invention discloses a kind of Trion X-100 enhanced biological charcoal immobilized microorganism materials and its preparation method and application.Using Trion X-100 enhanced biological charcoal to the eutectoid content of pollutant and degradation bacteria pseudomonas aeruginosa, to promote charcoal immobilized microorganism material on the whole to the removal efficiency of Organic Pollutants in Wastewater.The Triton X-100 enhanced biological charcoal immobilized microorganism material that the present invention is prepared has good Adsorption effect to the hardly degraded organic substance in waste water, and is significantly increased compared to traditional absorption method immobilized microorganism to the removal efficiency of organic matter.

Description

Triton X-100 enhanced biological charcoal immobilized microorganism material and preparation method thereof and Using
Technical field
The present invention relates to technical field of waste water processing, in particular to a kind of immobilized microorganism material for handling organic wastewater Preparation method and application.
Background technique
The efficient removal of persistence organic pollutant is always the hot spot of environmental science and engineering of water treatment research in waste water One of.Microbial degradation and adsorbent absorption are the approach of two kinds of most conventional processing organic wastewater, but simple degradation is imitated Rate is lower, simple absorption method is although high-efficient but processing is not thorough.Adsorbent process for immobilizing enzyme/microbe had both degradation and Two kinds of effects are adsorbed, can quickly reduce the organic matter in waste water, while providing high pollution object concentration for the microorganism of immobilization Microenvironment, conducive to its efficient degradation to pollutant.
Immobilized microorganism technique is that free microorganism is fixed on carrier using method chemically or physically, significantly The quantity of microorganism is increased, and keeps its activity and stability.
Charcoal (Biochar) is biomass (such as straw and animal wastes) the complete or partial anoxic the case where The carbonaceous material of the lower height aromatisation generated through pyrolysis.Charcoal has flourishing pore structure, specific surface because being rich in carbon A kind of excellent physicochemical properties such as long-pending big and ion exchange capacity height, it is considered to be efficient adsorbent.Charcoal simultaneously The microcellular structure on surface, which is also inhabited for microorganism, provides place with breeding, makes microorganism from the disturbance of unsuitable environmental condition. On the other hand, charcoal contains the nutrients such as a large amount of carbon, nitrogen, also provides nutriment for microorganism, the life to microorganism It is long to play certain positive effect with breeding.Therefore, charcoal is a kind of ideal fixation support.Currently, charcoal is fixed Change microorganism to have been used in the recovery technique of polluted-water and soil.
Common process for fixation includes absorption method, investment, cross-linking method and covalent coupling method.Wherein, absorption method operates Simply, at low cost, it is small on cell activity influence, it is one of most common method.But the shortcomings that absorption method, is carrier and micro- life Object binding force is lower, and effect is unstable.
Summary of the invention
The present invention is modified biological carbon surface using Trion X-100 (Triton X-100), strengthens life Object charcoal is to the eutectoid content of pollutant and degradation bacteria, while it is strong to the suction-operated of pollutant and the fixation of degradation bacteria to improve charcoal Degree promotes charcoal immobilized microorganism material to the mesh of the removal efficiency of Organic Pollutants in Wastewater to reach on the whole 's.
For achieving the above object, the present invention is implemented with the following technical solutions:
The present invention provides a kind of preparation method of Trion X-100 enhanced biological charcoal immobilized microorganism material, specific to walk It is rapid as follows: step 1) choose it is 30 DEG C of pseudomonas aeruginosa Pseudomonas aeruginosa JXQ a kind of under the conditions of be incubated at In the minimal medium of the acenaphthene containing 3.5mg/L, it is centrifuged after 24 hours, resuspension.
Pseudomonas aeruginosa Pseudomonas aeruginosa JXQ in the present invention was in preservation on December 28 in 2015 In China typical culture collection center, deposit number CCTCC NO:M2015786, preservation address is Wuhan, China Wuhan University.
Step 2) adjusts bacteria suspension using minimal medium, enables OD600In 0.1~0.5 range, Triton X- is added 100, enabling Triton X-100 concentration is 10mg/L~30mg/L.
Step 3) mixes charcoal with the P. aeruginosa bacteria suspension 1:100 in mass ratio of the X-100 containing Triton, and 30 It is stirred under the conditions of DEG C for 24 hours, charcoal immobilized microorganism material is obtained by filtration.
Preferably, above-mentioned steps 3) involved in charcoal be one or more of rice straw, bamboo powder, rice bran Under 500 DEG C of anoxia conditions made from cracking.
The present invention also provides a kind of Triton X-100 enhanced biological charcoal immobilized microorganism material, the microbial materials It is prepared by following preparation method:
Step 1) choose it is 30 DEG C of pseudomonas aeruginosa Pseudomonas aeruginosa JXQ a kind of under the conditions of be incubated at In the minimal medium of the acenaphthene containing 3.5mg/L, it is centrifuged after 24 hours, resuspension；
Step 2) adjusts bacteria suspension using minimal medium, enables OD600 in 0.1~0.5 range, and Triton is added X-100, enabling Triton X-100 concentration is 10mg/L~30mg/L；
Step 3) mixes charcoal with the P. aeruginosa bacteria suspension 1:100 in mass ratio of the X-100 containing Triton, and 30 It is stirred under the conditions of DEG C for 24 hours, charcoal immobilized microorganism material is obtained by filtration.
It is useless in removal that the present invention also provides a kind of above-mentioned Triton X-100 enhanced biological charcoal immobilized microorganism materials The application of acenaphthene in water.
Further, Triton X-100 enhanced biological charcoal immobilized microorganism material is direct plungeed into organic wastewater, is mixed After closing stirring 4h, the acenaphthene in waste water can remove.
The present invention enhances the intensity and efficiency of charcoal immobilized microorganism using the method for chemical strengthening, to improve life The practicability that object charcoal immobilized microorganism is applied in wastewater treatment and pollution waters restoration.Specifically, the present invention is creatively Using nonionic surfactant Trion X-100, increase the associativity between charcoal and microorganism, while charcoal being promoted to inhale Attached organic pollutant, to play the role of improving charcoal immobilized microorganism material on the whole to contaminant removal efficiency.
The preparation method of immobilized microorganism material of the present invention is simple, and energy consumption is few, and obtained Triton X-100 strengthens life Object charcoal immobilized microorganism material has the removal efficiency of useless Organic substance in water compared to traditional absorption method immobilized microorganism aobvious It writes and improves.
Specific embodiment
Below with reference to embodiment, present invention is further described in detail:
Embodiment 1
Pseudomonas aeruginosa Pseudomonas aeruginosa JXQ is incubated at acenaphthene containing 3.5mg/L under the conditions of 30 DEG C Minimal medium in, be centrifuged after 24 hours, resuspension.Bacteria suspension is adjusted with minimal medium, obtains OD600It is 0.1 Bacterium solution 100mL.The 10g/L Triton X-100 solution of 0.3mL is added in above-mentioned bacterium solution, and (Triton X-100 concentration is 30mg/L).1g charcoal is added in the 100mL bacterium solution of the above-mentioned X-100 containing Triton (mass ratio 1:100), under the conditions of 30 DEG C For 24 hours, charcoal immobilized microorganism material is obtained by filtration in stirring.
It will be mixed in the organic wastewater of the charcoal immobilized microorganism material 2.5g being prepared investment 1L 3.5mg/L containing acenaphthene Stirring 4h is closed, 87.4% can reach to the removal rate of acenaphthene.As control, the life prepared under Triton X-100 intensified condition is not added Object charcoal immobilized microorganism material is 62.0% to the removal rate of acenaphthene under similarity condition.As it can be seen that strong using Triton X-100 After change, immobilized microorganism material improves 40.9% to the removal rate of acenaphthene.
Embodiment 2
Pseudomonas aeruginosa Pseudomonas aeruginosa JXQ is incubated at acenaphthene containing 3.5mg/L under the conditions of 30 DEG C Minimal medium in, be centrifuged after 24 hours, resuspension.Bacteria suspension is adjusted with minimal medium, obtains OD600It is 0.2 Bacterium solution 100mL.The 10g/L Triton X-100 solution of 0.1mL is added in above-mentioned bacterium solution, and (Triton X-100 concentration is 10mg/L).1g charcoal is added in the 100mL bacterium solution of the above-mentioned X-100 containing Triton (mass ratio 1:100), under the conditions of 30 DEG C 12h is stirred, charcoal immobilized microorganism material is obtained by filtration.
It will be mixed in the organic wastewater of the charcoal immobilized microorganism material 2.5g being prepared investment 1L 3.0mg/L containing acenaphthene Stirring 4h is closed, 77.4% can reach to the removal rate of acenaphthene.As control, the life prepared under Triton X-100 intensified condition is not added Object charcoal immobilized microorganism material is 70.6% to the removal rate of acenaphthene under similarity condition.After being strengthened using Triton X-100, Immobilized microorganism material improves 9.6% to the removal rate of acenaphthene.
Embodiment 3
Pseudomonas aeruginosa Pseudomonas aeruginosa JXQ is incubated at acenaphthene containing 3.5mg/L under the conditions of 30 DEG C Minimal medium in, be centrifuged after 24 hours, resuspension.Bacteria suspension is adjusted with minimal medium, obtains OD600It is 0.2 Bacterium solution 100mL.The 10g/L Triton X-100 solution of 0.1mL is added in above-mentioned bacterium solution, and (Triton X-100 concentration is 10mg/L).1g charcoal is added in the 100mL bacterium solution of the above-mentioned X-100 containing Triton (mass ratio 1:100), under the conditions of 30 DEG C For 24 hours, charcoal immobilized microorganism material is obtained by filtration in stirring.
It will be mixed in the organic wastewater of the charcoal immobilized microorganism material 2.5g being prepared investment 1L 3.5mg/L containing acenaphthene Stirring 4h is closed, 87.7% can reach to the removal rate of acenaphthene.As control, the life prepared under Triton X-100 intensified condition is not added Object charcoal immobilized microorganism material is 77.3% to the removal rate of acenaphthene under similarity condition.As it can be seen that strong using Triton X-100 After change, immobilized microorganism material improves 13.5% to the removal rate of acenaphthene.
Embodiment 4
Pseudomonas aeruginosa Pseudomonas aeruginosa JXQ is incubated at acenaphthene containing 3.5mg/L under the conditions of 30 DEG C Minimal medium in, be centrifuged after 24 hours, resuspension.Bacteria suspension is adjusted with minimal medium, obtains OD600It is 0.5 Bacterium solution 100mL.The 10g/L Triton X-100 solution of 0.1mL is added in above-mentioned bacterium solution, and (Triton X-100 concentration is 10mg/L).1g charcoal is added in the 100mL bacterium solution of the above-mentioned X-100 containing Triton (mass ratio 1:100), under the conditions of 30 DEG C For 24 hours, charcoal immobilized microorganism material is obtained by filtration in stirring.
It will be mixed in the organic wastewater of the charcoal immobilized microorganism material 2.5g being prepared investment 1L 3.5mg/L containing acenaphthene Stirring 4h is closed, 83.9% can reach to the removal rate of acenaphthene.As control, the life prepared under Triton X-100 intensified condition is not added Object charcoal immobilized microorganism material is 66.8% to the removal rate of acenaphthene under similarity condition.As it can be seen that strong using Triton X-100 After change, immobilized microorganism material improves 25.6% to the removal rate of acenaphthene.
In short, the foregoing is merely presently preferred embodiments of the present invention, it is all according to equalization made by scope of the present invention patent Variation and modification, shall all be covered by the patent of the invention.

Claims (6)

  1. The preparation method of 1.Triton X-100 enhanced biological charcoal immobilized microorganism material, which is characterized in that the preparation side Steps are as follows for method:
    Step 1) choose it is 30 DEG C of pseudomonas aeruginosa Pseudomonas aeruginosa JXQ a kind of under the conditions of be incubated at and contain In the minimal medium of 3.5mg/L acenaphthene, it is centrifuged after 24 hours, resuspension；
    Step 2) adjusts bacteria suspension using minimal medium, enables OD600 in 0.1~0.5 range, and Triton X- is added 100, enabling Triton X-100 concentration is 10mg/L~30mg/L；
    Step 3) mixes charcoal with the P. aeruginosa bacteria suspension 1:100 in mass ratio of the X-100 containing Triton, 30 DEG C of items It is stirred under part for 24 hours, charcoal immobilized microorganism material is obtained by filtration；
    Pseudomonas aeruginosa Pseudomonas aeruginosa JXQ is preserved in Chinese Typical Representative culture guarantor in the step 1) Hiding center, deposit number CCTCC NO:M2015786.
  2. 2. the preparation method of Triton X-100 enhanced biological charcoal immobilized microorganism material according to claim 1, Be characterized in that: charcoal is one of rice straw, bamboo powder, rice bran or a variety of in 500 DEG C of anoxia conditions in the step 3) Made from lower cracking.
  3. 3. a kind of Triton X-100 enhanced biological charcoal immobilized microorganism material, which is characterized in that the microbial material is logical Following preparation method is crossed to be prepared:
    Step 1) choose it is 30 DEG C of pseudomonas aeruginosa Pseudomonas aeruginosa JXQ a kind of under the conditions of be incubated at and contain In the minimal medium of 3.5mg/L acenaphthene, it is centrifuged after 24 hours, resuspension；
    Step 2) adjusts bacteria suspension using minimal medium, enables OD600 in 0.1~0.5 range, and Triton X- is added 100, enabling Triton X-100 concentration is 10mg/L~30mg/L；
    Step 3) mixes charcoal with the P. aeruginosa bacteria suspension 1:100 in mass ratio of the X-100 containing Triton, 30 DEG C of items It is stirred under part for 24 hours, charcoal immobilized microorganism material is obtained by filtration；
    Pseudomonas aeruginosa Pseudomonas aeruginosa JXQ is preserved in Chinese Typical Representative culture guarantor in the step 1) Hiding center, deposit number CCTCC NO:M2015786.
  4. 4. Triton X-100 enhanced biological charcoal immobilized microorganism material according to claim 3, it is characterised in that: institute Stating charcoal in step 3) is that one of rice straw, bamboo powder, rice bran or a variety of crack under 500 DEG C of anoxia conditions are made 's.
  5. 5. a kind of Triton X-100 enhanced biological charcoal immobilized microorganism material as claimed in claim 3 is in removal waste water The application of acenaphthene.
  6. 6. application according to claim 5, which is characterized in that by Triton X-100 enhanced biological charcoal immobilized microorganism Material direct plunges into organic wastewater, after 4h is mixed, can remove the acenaphthene in waste water.
CN201610132948.1A 2016-03-09 2016-03-09 Triton X-100 enhanced biological charcoal immobilized microorganism material and its preparation method and application Active CN105695443B (en)

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CN106754513B (en) * 2016-12-22 2020-08-14 浙江工商大学 Preparation and application of TX-100 modified sodium alginate embedded pseudomonas particles
CN107893064B (en) * 2017-11-16 2021-07-16 信阳师范学院 Preparation method and application of biochar-polyvinyl alcohol combined immobilized microalgae pellets
CN109365495A (en) * 2018-12-05 2019-02-22 辽宁石油化工大学 The method of charcoal carrier surface activating agent coupling antimicrobial plant remedying oil-polluted soils
CN112246228A (en) * 2020-10-31 2021-01-22 兰州资源环境职业技术学院 Preparation method of modified graphene material for removing heavy metal pollution in soil
CN115156263B (en) * 2022-07-07 2023-10-27 辽宁石油化工大学 Method for restoring polluted soil by utilizing graphene oxide reinforced pseudomonas aeruginosa

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