CN109468307A - Immobilization microorganism particles and preparation method thereof and the method that black and odorous water is efficiently administered with immobilization microorganism particles - Google Patents
Immobilization microorganism particles and preparation method thereof and the method that black and odorous water is efficiently administered with immobilization microorganism particles Download PDFInfo
- Publication number
- CN109468307A CN109468307A CN201811292667.8A CN201811292667A CN109468307A CN 109468307 A CN109468307 A CN 109468307A CN 201811292667 A CN201811292667 A CN 201811292667A CN 109468307 A CN109468307 A CN 109468307A
- Authority
- CN
- China
- Prior art keywords
- immobilization
- microorganism particles
- black
- microorganism
- immobilization microorganism
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
- C12N11/12—Cellulose or derivatives thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Inorganic Chemistry (AREA)
- Biological Treatment Of Waste Water (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The method of black and odorous water is efficiently administered the invention discloses a kind of immobilization microorganism particles and preparation method thereof and with immobilization microorganism particles.The good microbial material of preconcentration is added in dry calcium peroxide powder, adhesive is added to be sufficiently mixed uniformly, using direct power compressing method or compressing dry granulation, round, column type or square particle is made, spontaneously dries and obtains immobilization microorganism particles.The degradation bacteria for having high-efficient purification effect to black and odorous water is embedded in the immobilization microorganism particles that calper calcium peroxide, adhesive and microbial material are mixed by the present invention, while keeping higher bacteria concentration, the dissolved oxygen in black smelly waste water can be quickly improved, realizes the high-efficient purification of black and odorous water.This method treatment effect is stable, does not generate secondary pollution, is readily transported and solves the problems, such as that black and odorous water dissolved oxygen is low, microbial inoculum treatment effeciency is low simultaneously.The technique to high-efficiency and it is practical, huge effect will be played in terms of environmental project is administered.
Description
Technical field
The invention belongs to administer the water-treatment technology field of black and odorous water with immobilized microorganism, it is micro- to be related to a kind of immobilization
Biologic grain and preparation method thereof, and the method for efficiently administering black and odorous water with the immobilization microorganism particles.
Background technique
Having its source in Sediments for black and odorous water is enriched a large amount of pollutant, such as organic matter and nitrogen and phosphorus object
Matter, these polluters are constantly discharged to water body upper layer, become the secondary pollution source of water pollution.Have to Sediments
The improvement of effect keeps it final completely inorganization, is the effective way for fundamentally solving black and odorous water pollution.
Technology currently used for black-odor riverway reparation is mainly peripheral doses technology, chemical remediation technology and biological prosthetic skill
Art, and bioremediation technology has the advantages that environmental-friendly, ecological energy-saving, is recovery technique most with prospects.Biology is repaired
Refer to the pollutant in the river that absorbs, converts, removes or degrade using specifically biology again, to enable polluted water body
It is restored to the biological control measure of original position partially or completely.Bioremediation technology is usually required using a large amount of microbial inoculums and a phase
To nutrition-balanced environmental condition.Existing bioremediation technology is usually that the throwing of a large amount of microbial inoculums is spread in black and odorous water, leans on bacterium
Agent restores water body with self-reparing capability from domestication, or artificial by increasing water body aeration increase Dissolved Oxygen in Water, changes
Kind condition of water quality, improves biological treatment efficiency, but the problem is that microbial inoculum be lost with water body it is serious, cannot be in the limited time
Or bring about the desired effect in basin perimeter, in addition, Dissolved Oxygen in Water deficiency is that restriction microorganism plays effect and water body is net
The principal element of change.
Immobilized microorganism technique is the means and method with chemically or physically, by free cell or electrodes method to certain
Within the scope of one particular space, the modern biological project skill that retains its intrinsic catalytic activity, and can be repeated and be used continuously
Art.Immobilized microorganism technique overcomes that biological cell is too small, and it is more difficult to separate with aqueous solution, easily causes the shortcomings that outflowing with water,
And have the advantages that high-efficient, stability is strong and keeps high-efficiency strain.Currently, this technology is widely used in water process neck
Domain becomes research hotspot.
Process for immobilizing enzyme/microbe has physics fixation and chemical fixation.Physics fixation mainly has investment, inhales
Attached method, envelope method.Chemical fixation includes covalent coupling method, cross-linking method.Investment be microorganism is wrapped in gel grid or
In polymer semi-permeable membrane microcapsules.Absorption method is to be arrived microorganism adsorption using the adsorbent (carrier) with height adsorption capacity
Immobilization is allowed on its surface.Both methods is simple and easy to do, with it is more.
In immobilized microorganism technique, the dominant bacteria for the special efficacy degradation bacteria that microorganism is mainly artificially selected, immobilization
Carrier is that microorganism creates the living environment for being not easy to disintegrate, the attachment fixation of microorganism and growth metabolism all largely by
Number to the influence of carrier material, microbial biomass is also related with the structure of carrier material.So an ideal immobilization carries
The selection of body is critically important.Existing microbial fixed carrier includes inorganic carrier, organic polymer carrier, complex carrier.Often
Inorganic carrier material mainly has cellular glass, diatomite, quartz sand, zeolite, active carbon, rice husk etc..It is common organic high
Molecular vehicle material has sodium alginate, chitosan, starch, cellulose, polyvinyl alcohol, polyacrylamide, carbopol gel, light
Hardening resin etc..Complex carrier is the carrier material that inorganic carrier and organic polymer carrier are combined.
Existing immobilized microorganism technique has certain effect in terms of handling the less serious water body of organic contamination, still
There are some defects in terms of the black and odorous water for administering organic contamination especially severe, effect is unsatisfactory, main reason is that water
Body dissolved oxygen is low (aeration condition of especially bed mud is poor), and microorganism, which is not easy to play it in the low water body environment of oxygen content, to answer
Some activity and effect cause microbiological treatment effect poor.Therefore, it is necessary to seek a kind of microorganism can be made to play in water body
The method that black and odorous water is efficiently administered with immobilized microorganism technique of high activity out.
Summary of the invention
It is an object of the invention to: a kind of immobilized microorganism that microorganism can be made to play high activity in water body is provided
Particle and a kind of method for efficiently administering black and odorous water with the immobilization microorganism particles.
Technical concept of the invention is as follows:
CaO2(calper calcium peroxide) has been caused as a kind of material for having both oxygen release and oxidisability in environment remediation field
More and more concerns are having huge application prospect for repairing in contaminated surrounding medium.Calper calcium peroxide energy and water
Middle copper, zinc, iron, phosphorus etc. in conjunction with and mitigate water body toxicity, promote bottom of pond anaerobic bacteria flora to decompose the mineralising and humus of organic matter,
Precipitate the colloidal solid to suspend in water, transparency increases, and water quality grows fat, lives, feels well, and is conducive to planktonic organism breeding, keeps good
Good ecological environment.Calper calcium peroxide, which meets water, can slowly generate calcium hydroxide, and it is heavy that calcium hydroxide absorbing carbon dioxide generates calcium carbonate
It forms sediment, calcium carbonate can make mud at open structure, improve the aeration condition of bed mud, accelerate the effect of bacterial decomposition organic matter, and energy
Release makes water grow fat by the elements such as nitrogen, phosphorus, potassium that mud adsorbs, and accelerates the numerous of wheel animalcule in bottom of pond mud layer and its resting egg
It educates and grows, and fertilization effect can be played.The oxygen release of calper calcium peroxide, which is mainly reflected in it, which meets water, to go out oxygen with quick release.
Inventors have seen that calper calcium peroxide and the microbial material of immobilization are passed through viscous using the above-mentioned characteristic of calper calcium peroxide
Mixture is combined together, and immobilization microorganism particles are made, and the microbe granular of this immobilization is then put into black and odorous water
In for administering black and odorous water, on the one hand can improve the low situation of Dissolved Oxygen in Water, on the other hand can also make calper calcium peroxide in water
It is slowly dissolved release oxygen in body, increases dissolubility and bioactivity of the microorganism in water body, microorganism thus can be improved
Purified treatment effect.
Technical scheme is as follows:
A kind of immobilization microorganism particles of the present invention, wherein each component and mass percent are as follows:
Calper calcium peroxide 50~90%
Adhesive 1~45%
Microbial material 3~25%；
The microbial material is that good (after the concentration) high concentration microorganism of preconcentration is solidificated in carrier material
Made of upper；The microorganism concn being enriched in the microbial material is 108~1011A/gram；
The calper calcium peroxide is dry calcium peroxide powder；
The immobilization microorganism particles are that the microbial material for being enriched with microorganism is added to dry peroxidating
In calcium powder, adhesive is added, is sufficiently mixed uniformly, using direct power compressing method or compressing dry granulation, tabletting,
Granulation, manufactured dry particle.
Further, the calcium peroxide powder of the drying is dehydration 1-48 hours dry under the conditions of 25-100 DEG C, grain
Degree is the calcium peroxide powder of 50-500 mesh.
Further, the adhesive is amylum pregelatinisatum, in spray-dried lactose, microcrystalline cellulose, povidone
It is one or more of.
Further, the carrier material is inorganic carrier material, organic polymer carrier material or complex carrier material
Material.The inorganic carrier material is at least one of cellular glass, diatomite, quartz sand, zeolite, active carbon, rice husk etc.
(preferably active carbon, rice husk).The organic polymer carrier material is sodium alginate, chitosan, starch, cellulose, poly- second
At least one of enol, polyacrylamide, carbopol gel, light-hardening resin etc..
Further, the microorganism is bacillus, nitrobacteria, denitrifying bacteria, COD degradation bacteria, ammonia nitrogen drop
Solve at least one of aerobic bacterias such as bacterium, nitrite nitrogen degradation bacteria, dephosphorization bacterial, sulphur degradation bacteria.
Further, the microorganism is the degradation bacteria for having high-efficient purification effect to black and odorous water, preferably to black smelly water
The compound bacteria that pollutant in body has efficient degradation to act on.
A kind of preparation method of immobilization microorganism particles of the present invention, sequentially including the following steps: (1) will be preparatory
On a support material, microbial material is made in the high concentration microorganism solidification being enriched with；(2) by the dry dehydration of calper calcium peroxide, mistake
Sieve obtains the calcium peroxide powder that granularity is 50-500 mesh；(3) in the ratio, microbial material is added to dry mistake
It in lime powder, is sufficiently mixed, adds adhesive and be uniformly mixed, using direct power compressing method or dry granulation tabletting
Particle is made in method, tabletting, spontaneously dries, and obtains immobilization microorganism particles.
Further, in above-mentioned steps (1), microbial material is prepared method particularly includes: by microorganism (to black and odorous water
In pollutant have efficient degradation act on compound bacteria) bacterium solution centrifugal concentrating or cross film concentration, by spray drying be solidificated in work
Property the carrier materials such as charcoal, rice husk on, microbial material is made, the microorganism concn being enriched in manufactured microbial material is 108
~1011A/gram.
Dehydration 1-48 hours dry under the conditions of 25-100 DEG C by calper calcium peroxide in above-mentioned steps (2), grain is made in sieving
Degree is the calcium peroxide powder of 50-500 mesh；
In above-mentioned steps (3), round, column type or square particle is made in tabletting；After natural drying, the immobilization of acquisition is micro-
Biologic grain moisture content is lower than 5%.
There are two ways to efficiently administering black and odorous water with above-mentioned manufactured immobilization microorganism particles: one is will be upper
Manufactured immobilization microorganism particles are stated, are added directly in black and odorous water in the ratio that adds of the weight ratio of 0.1-10%；Or
Person, another kind be, will it is above-mentioned made of immobilization microorganism particles, the aerobic reactor zone of bioreactor is packed into as filler
(instead of " filler+aerobic bacteria " in protozoa reactor), which is placed in black and odorous water, is carried out biological reinforced
Reaction, (the suitable reaction temperature of microorganism is 20 DEG C -40 DEG C).
It should be noted that the domestication of objective microbe bacterium, to expand the processing techniques such as numerous be the prior art, the common skill in this field
Art personnel can realize that the present invention is herein without limiting using the prior art.In addition, concentration generally include film concentration, from
Heart concentration etc., is concentrated and the high concentration microorganism after concentration is equally solidificated in active carbon, rice for conventional technical means in the art
It is also the ordinary skill in the art on the carrier materials such as shell, technical staff can realize that the present invention is herein using the prior art
It is not especially limited.
Beneficial effects of the present invention:
The present invention provides a kind of, and that microbial material is added to manufactured immobilization in dry calcium peroxide powder is micro-
Biologic grain and a kind of method for administering black and odorous water with the immobilization microorganism particles.The present invention not only provides one kind
Microorganism immobilization method, also to improve, Dissolved Oxygen in Water is low, microbiological treatment effect difference provides a new solution.
Manufactured immobilization microorganism particles in the present inventive method, can improve that Dissolved Oxygen in Water is low, poor processing effect of microorganism
The problem of, it can make microorganism play high activity in water body, thus can greatly improve the treatment effect of microorganism.It is sent out with this
Bright immobilization microorganism particles can administer black and odorous water, and treatment effect is stable, does not generate secondary pollution, be readily transported, and
Can solve the problems, such as that black and odorous water dissolved oxygen is low, microbial inoculum treatment effeciency is low simultaneously.It is contemplated that the technology will become environment work
A Xiang Gaoxiao of Cheng Zhili and practical technology, and huge effect will be played.
The present invention has at least the following advantages:
First aspect, the invention belongs to biochemical processings, will there is the degradation bacterium bag of high-efficient purification effect to black and odorous water
It is embedded in the immobilization microorganism particles that calper calcium peroxide, adhesive and microbial material are mixed, is keeping higher bacteria concentration
While, the dissolved oxygen in black smelly waste water can be quickly improved, realizes the high-efficient purification of black and odorous water.
Second aspect, the present invention remove the pollution in water body using calper calcium peroxide and the adhesive that can be utilized by microorganism
Object, treatment effect is stable, efficiently and does not generate secondary pollution.
The third aspect, the present invention can be added directly in bioreactor using immobilization microorganism particles for biology
Strengthen reaction, there is good bioactivity and hydraulic intensity, there is preferable clean-up effect to black and odorous water.
Fourth aspect, the present invention use microbial immobilized effect, have possessed by conventional immobilization microorganism particles
The features such as high bacteria suspension concentration, impact resistance are strong, adaptable.
5th aspect, the present invention can be combined with traditional handicraft, and traditional biological work is participated in the form of microbial inoculum filler
In skill, there is popularizing value.
Specific embodiment
A kind of immobilization microorganism particles of the present invention are described further below with reference to specific embodiment.
The microorganism used in the following embodiment for having high-efficient purification effect to black and odorous water is the construction of Shanghai water head site
The compound bacteria for having efficient degradation to act on the pollutant in black and odorous water that Development Co., Ltd's domestication obtains (is wrapped in the compound bacteria
Containing a large amount of nitrobacteria, denitrifying bacteria and ammonia nitrogen degradation bacterium).
Embodiment 1
(1) a kind of immobilization microorganism particles of the present invention, wherein each component and mass percent are as follows:
Dry calcium peroxide powder 70%
Adhesive 27%
Microbial material 3%；
The microbial material is that good (after the concentration) high concentration microorganism of preconcentration is solidificated in carrier material
Made of upper；The microorganism concn being enriched in the microbial material is 108~1011A/gram.
The carrier material is active carbon；The adhesive is amylum pregelatinisatum.
(2) above-mentioned immobilization microorganism particles the preparation method is as follows:
(1) microbial material is made in enriched microorganism: will have answering for efficient degradation effect to the pollutant in black and odorous water
Combined bacteria bacterium solution centrifugal concentrating, by spray drying solidification on the activated carbon, microbial material is made, make wherein microorganism concn reach
To 108~1011A/gram.
(2) preparation of dry calper calcium peroxide: by the calper calcium peroxide selected and purchased on the market, dry dehydration 3 is small under the conditions of 60 DEG C
When, it is 200 mesh powders that granularity, which is made, in sieving.
(3) micro- life the preparation of immobilization microorganism particles: is added in dry calcium peroxide powder by formula rate
Round particle is made in object material and amylum pregelatinisatum, the compression molding on tablet press machine, and drying at room temperature 48h obtains the micro- life of immobilization
Composition granule (moisture content is lower than 5%).
(3), black and odorous water, test method and data result are efficiently administered with above-mentioned manufactured immobilization microorganism particles:
Wastewater source: the black smelly water of Shanghai Shuiyuandi Construction Development Co., Ltd.'s river black and odorous water administration project, ammonia
Nitrogen concentration is 8mg/L, CODCrConcentration is 21.5mg/L, DO 0.5mg/L.
The immobilization microorganism particles for taking 8g above-mentioned steps (3) to prepare are placed in the black smelly water of 300mL shake culture at 30 DEG C
Processing, every ammonia nitrogen, DO, COD in 12 hours sample detection waste waterCrValue, corresponding concentration are respectively as follows:
Embodiment 2
(1) a kind of immobilization microorganism particles of the present invention, wherein each component and mass percent are as follows:
Dry calcium peroxide powder 50%
Adhesive 45%
Microbial material 5%；
The microbial material is that good (after the concentration) high concentration microorganism of preconcentration is solidificated in carrier material
Made of upper；The microorganism concn being enriched in the microbial material is 108~1011A/gram.
The carrier material is rice husk；The adhesive is microcrystalline cellulose.
(2) above-mentioned immobilization microorganism particles the preparation method is as follows:
(1) microbial material is made in enriched microorganism: will have answering for efficient degradation effect to the pollutant in black and odorous water
Combined bacteria bacterium solution centrifugal concentrating, by spray drying be solidificated on rice husk, microbial material is made, make wherein microorganism concn reach
108~1011A/gram.
(2) preparation of dry calper calcium peroxide: the dry dehydration 1 under the conditions of 100 DEG C by the calper calcium peroxide selected and purchased on the market
Hour, it is 500 mesh powders that granularity, which is made, in sieving.
(3) preparation of immobilization microorganism particles: pressing formula rate, and in dry calcium peroxide powder, the micro- life of 25g is added
Object material and 8g microcrystalline cellulose are uniformly mixed, the compression molding on tablet press machine, and square particle is made, and drying at room temperature 48h is obtained
Immobilization microorganism particles (moisture content is lower than 5%).
(3), black and odorous water is efficiently administered with above-mentioned manufactured immobilization microorganism particles, tested
Method and data result:
Wastewater source: the black smelly water of Shanghai Shuiyuandi Construction Development Co., Ltd.'s river black and odorous water administration project, ammonia
Nitrogen concentration is 8mg/L, CODCrConcentration is 21.5mg/L, DO 0.5mg/L.
The immobilization microorganism particles for taking 8g above-mentioned steps (3) to prepare are placed in the black smelly water of 300mL shake culture at 30 DEG C
Processing, every ammonia nitrogen, DO, COD in 12 hours sample detection waste waterCrValue, corresponding concentration are respectively as follows:
Embodiment 3
(1) a kind of immobilization microorganism particles of the present invention, wherein each component and mass percent are as follows:
Dry calcium peroxide powder 90%
Adhesive 1%
Microbial material 9%；
The microbial material is that good (after the concentration) high concentration microorganism of preconcentration is solidificated in carrier material
Made of upper；The microorganism concn being enriched in the microbial material is 108~1011A/gram.
The carrier material is diatomite；The adhesive is povidone.
(2) above-mentioned immobilization microorganism particles the preparation method is as follows:
(1) microbial material is made in enriched microorganism: will have answering for efficient degradation effect to the pollutant in black and odorous water
Combined bacteria bacterium solution cross film concentration, by spray drying solidification over celite, microbial material is made, make wherein microorganism concn reach
To 108~1011A/gram.
(2) preparation of calper calcium peroxide: by the calper calcium peroxide selected and purchased on the market dry dehydration 48 hours under the conditions of 25 DEG C,
It is 50 mesh powders that granularity, which is made, in sieving.
(3) micro- life the preparation of immobilization microorganism particles: is added in dry calcium peroxide powder by formula rate
Object material and povidone are uniformly mixed, the compression molding on tablet press machine, and column type particle is made, and drying at room temperature 48h obtains immobilization
Microbe granular (moisture content is lower than 5%).
(3), black and odorous water is efficiently administered with above-mentioned manufactured immobilization microorganism particles, tested
Method and data result:
Wastewater source: the black smelly water of Shanghai Shuiyuandi Construction Development Co., Ltd.'s river black and odorous water administration project, ammonia
Nitrogen concentration is that 8mg/L, CODCr concentration are 21.5mg/L, DO 0.5mg/L.
The immobilization microorganism particles for taking 8g above-mentioned steps (3) to prepare are placed in the black smelly water of 300mL shake culture at 30 DEG C
Processing, is respectively as follows: every ammonia nitrogen, DO, CODCr value, corresponding concentration in 12 hours sample detection waste water
Embodiment 4
(1) a kind of immobilization microorganism particles of the present invention, wherein each component and mass percent are as follows:
Dry calcium peroxide powder 60%
Adhesive 25%
Microbial material 15%；
The microbial material is that good (after the concentration) high concentration microorganism of preconcentration is solidificated in carrier material
Made of upper；The microorganism concn being enriched in the microbial material is 108~1011A/gram.
The carrier material is chitosan；The adhesive is spray-dried lactose.
(2) above-mentioned immobilization microorganism particles the preparation method is as follows:
(1) microbial material is made in enriched microorganism: will have answering for efficient degradation effect to the pollutant in black and odorous water
Combined bacteria bacterium solution cross film concentration, by spray drying be solidificated on chitosan, microbial material is made, make wherein microorganism concn reach
To 108~1011A/gram.
(2) preparation of dry calper calcium peroxide: by the calper calcium peroxide selected and purchased on the market, dry dehydration 6 is small under the conditions of 50 DEG C
When, it is 100 mesh powders that granularity, which is made, in sieving.
(3) micro- life the preparation of immobilization microorganism particles: is added in dry calcium peroxide powder by formula rate
Object material and spray-dried lactose are sufficiently mixed uniformly, and round particle, drying at room temperature is made in the compression molding on tablet press machine
48h obtains immobilization microorganism particles (moisture content is lower than 5%).
(3), black and odorous water is efficiently administered with above-mentioned manufactured immobilization microorganism particles, tested
Method and data result:
Wastewater source: the black smelly water of Shanghai Shuiyuandi Construction Development Co., Ltd.'s river black and odorous water administration project, ammonia
Nitrogen concentration is that 8mg/L, CODCr concentration are 21.5mg/L, DO 0.5mg/L.
The immobilization microorganism particles for taking 8g above-mentioned steps (3) to prepare are placed in the black smelly water of 300mL shake culture at 30 DEG C
Processing, is respectively as follows: every ammonia nitrogen, DO, CODCr value, corresponding concentration in 12 hours sample detection waste water
Embodiment 5 (comparative experimental example)
A kind of immobilization microorganism particles that calper calcium peroxide is not added are prepared, with the fixation for adding calper calcium peroxide in embodiment 4
Change microbe granular comparison, compares experiment.
(1) this immobilization microorganism particles that calper calcium peroxide is not added are by microbial material and adhesive by the matter of 15:25
Amount ratio is mixed, and preparation method is as follows:
(1) microbial material is made in enriched microorganism: the compound bacteria liquid centrifugation for having efficient degradation to act in nitrogen is dense
Contracting, by spray drying be solidificated on chitosan, microbial material is made, make wherein microorganism concn reach 108~1011A/
g。
(2) preparation of immobilization microorganism particles: microbial material and spray-dried lactose are filled by the mass ratio of 15:25
Divide and be uniformly mixed, round particle is made in the compression molding on tablet press machine, and drying at room temperature 48h obtains immobilization microorganism particles
(moisture content is lower than 5%).
(2), black and odorous water is efficiently administered with above-mentioned manufactured immobilization microorganism particles, tested
Method and data result:
Wastewater source: the black smelly water of Shanghai Shuiyuandi Construction Development Co., Ltd.'s river black and odorous water administration project, ammonia
Nitrogen concentration is 8mg/L, CODCrConcentration is 21.5mg/L, DO 0.5mg/L.
The immobilization microorganism particles for taking 8g above-mentioned steps (2) to prepare are placed in the black smelly water of 300mL shake culture at 30 DEG C
Processing, every ammonia nitrogen, DO, COD in 12 hours sample detection waste waterCrValue, corresponding concentration are respectively as follows:
Comparative experiments conclusion:
After the experimental data of the experimental data of embodiment 4 and embodiment 5 is compared it is found that in microbial material and bonding
In the case that the mass ratio of agent is 15:25, the immobilization microorganism particles of calper calcium peroxide are added than not adding calper calcium peroxide
Immobilization microorganism particles have following bigger advantage:
(1) more preferable to the overall removal effect of ammonia nitrogen in black and odorous water, also, with the extension of time, effect difference becomes
Greatly, that is to say, that the time is longer, and effect difference is bigger, this to be more obvious to the better advantage of ammonia nitrogen removal effect.
The immobilization microorganism particles of calper calcium peroxide are added, after handling 60h, ammonia nitrogen value can be reduced to 0, it is substantially all to go
It removes.Immobilization microorganism particles without adding calper calcium peroxide still cannot be by whole removals after handling 72h, and ammonia nitrogen value is also
1.7mg/L。
(2) to COD in black and odorous waterCrReducing effect it is more preferable, also, with the extension of time, effect difference becomes larger,
That is the time is longer, effect difference is bigger, this to CODCrThe better advantage of reducing effect is more obvious.
The immobilization microorganism particles of calper calcium peroxide are added, it, can be by COD after handling 36hCrValue drop to 13-14mg/L it
Between.Immobilization microorganism particles without adding calper calcium peroxide, after handling 72h, CODCrValue is still in 17mg/L or more.
(3) more preferable to the promotion effect of dissolved oxygen in black and odorous water (DO), also, with the extension of time, effect difference
Become larger, that is to say, that the time is longer, and effect difference is bigger, this to be more obvious to the DO promotion better advantage of effect.
Add calper calcium peroxide immobilization microorganism particles, handle 36h after, can by DO value promoted to 2.6mg/L with
On.Immobilization microorganism particles without adding calper calcium peroxide, after handling 72h, DO value is still in 2.1mg/L or less.
It can be seen that the immobilization microorganism particles of addition calper calcium peroxide of the invention, can improve Dissolved Oxygen in Water it is low,
The problem of poor processing effect of microorganism, it can make microorganism play high activity in water body, thus can greatly improve micro- life
The treatment effect of object.
Claims (10)
1. a kind of immobilization microorganism particles, which is characterized in that wherein each component and mass percent are as follows:
Calper calcium peroxide 50~90%
Adhesive 1~45%
Microbial material 3~25%；
The microbial material is will be made of the good high concentration microorganism solidification on a support material of preconcentration；Described
The microorganism concn being enriched in microbial material is 108~1011A/gram；
The calper calcium peroxide is dry calcium peroxide powder；
The immobilization microorganism particles are that the microbial material for being enriched with microorganism is added to dry peroxidating calcium powder
In end, adhesive is added, is sufficiently mixed uniformly, using direct power compressing method or compressing dry granulation, tabletting, system
Grain, manufactured dry particle.
2. a kind of immobilization microorganism particles according to claim 1, which is characterized in that the peroxidating calcium powder of the drying
End is drying dehydration 1-48 hour under the conditions of 25-100 DEG C, and granularity is the calcium peroxide powder of 50-500 mesh.
3. a kind of immobilization microorganism particles according to claim 1, which is characterized in that the adhesive is compressibility
One or more of starch, spray-dried lactose, microcrystalline cellulose, povidone.
4. a kind of immobilization microorganism particles according to claim 1, which is characterized in that the carrier material is inorganic
Carrier material, organic polymer carrier material or composite carrier.
5. a kind of immobilization microorganism particles according to claim 4, which is characterized in that the inorganic carrier material is more
At least one of hole glass, diatomite, quartz sand, zeolite, active carbon, rice husk；The organic polymer carrier material is
Sodium alginate, chitosan, starch, cellulose, polyvinyl alcohol, polyacrylamide, carbopol gel, in light-hardening resin extremely
Few one kind.
6. a kind of immobilization microorganism particles according to claim 1, which is characterized in that the microorganism is gemma bar
Bacterium, nitrobacteria, denitrifying bacteria, COD degradation bacterium, ammonia nitrogen degradation bacterium, nitrite nitrogen degradation bacteria, dephosphorization bacterial, in sulphur degradation bacteria
At least one.
7. a kind of immobilization microorganism particles according to claim 1, which is characterized in that the microorganism is to black smelly water
The compound bacteria that pollutant in body has efficient degradation to act on.
8. a kind of preparation method of immobilization microorganism particles as described in claim 1, which is characterized in that according to the following steps into
Row: (1) by preconcentration good high concentration microorganism solidification on a support material, microbial material is made；(2) by calper calcium peroxide
Dry dehydration, sieving obtain the calcium peroxide powder that granularity is 50-500 mesh；(3) in the ratio, microbial material is added
It into dry calcium peroxide powder, is sufficiently mixed, adds adhesive and be uniformly mixed, using direct power compressing method or do
Particle is made in method granulating tabletting process, tabletting；It spontaneously dries, obtains immobilization microorganism particles.
9. a kind of preparation method of immobilization microorganism particles according to claim 8, which is characterized in that the step (1)
In, prepare microbial material method particularly includes: by microbial inoculum centrifugal concentrating or cross film concentration, solidify by spray drying
On a support material, microbial material is made, the microorganism concn being enriched in manufactured microbial material is 108~1011A/
Gram；In above-mentioned steps (2), dehydration 1-48 hours dry under the conditions of 25-100 DEG C by calper calcium peroxide, sieving, granularity, which is made, is
The calcium peroxide powder of 50-500 mesh；In the step (3), round, column type or square particle is made in tabletting；After natural drying,
The immobilization microorganism particles moisture content of acquisition is lower than 5%.
10. a kind of method for efficiently administering black and odorous water with immobilization microorganism particles as claimed in claim 1,
Be characterized in that, by the immobilization microorganism particles, in 0.1-10% weight ratio add ratio be added directly it is black smelly
In water body；Alternatively, the immobilization microorganism particles are packed into the aerobic reactor zone of bioreactor as filler, it will
The bioreactor is placed in black and odorous water, carries out biological reinforced reaction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811292667.8A CN109468307A (en) | 2018-11-01 | 2018-11-01 | Immobilization microorganism particles and preparation method thereof and the method that black and odorous water is efficiently administered with immobilization microorganism particles |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811292667.8A CN109468307A (en) | 2018-11-01 | 2018-11-01 | Immobilization microorganism particles and preparation method thereof and the method that black and odorous water is efficiently administered with immobilization microorganism particles |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109468307A true CN109468307A (en) | 2019-03-15 |
Family
ID=65672424
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811292667.8A Pending CN109468307A (en) | 2018-11-01 | 2018-11-01 | Immobilization microorganism particles and preparation method thereof and the method that black and odorous water is efficiently administered with immobilization microorganism particles |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109468307A (en) |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110015824A (en) * | 2019-04-11 | 2019-07-16 | 江南大学 | A kind of soft clay microorganism curing agent, the application in soft clay solidifies and application method |
| CN110818202A (en) * | 2019-11-28 | 2020-02-21 | 中冶南方都市环保工程技术股份有限公司 | Method for restoring water ecological system and water ecological system stepped restoration system |
| CN110845024A (en) * | 2019-12-10 | 2020-02-28 | 清华大学 | Underground water remediation simulation experiment system and simulation remediation method |
| CN110937696A (en) * | 2019-12-24 | 2020-03-31 | 河北省科学院生物研究所 | Composite material and method for removing nitrogen and phosphorus in wastewater |
| CN111072132A (en) * | 2020-01-09 | 2020-04-28 | 北京恩菲环保股份有限公司 | Sulfur-iron autotrophic denitrification suspended filler and preparation method thereof |
| CN111484123A (en) * | 2020-05-06 | 2020-08-04 | 北京工业大学 | Efficient nitrifying embedded bioactive filler and preparation method thereof |
| CN111559801A (en) * | 2020-05-06 | 2020-08-21 | 中国城市建设研究院有限公司 | Self-oxygen-release filler for repairing black and odorous water body and preparation method and application thereof |
| CN112604667A (en) * | 2020-11-25 | 2021-04-06 | 南京乐透思环保科技有限公司 | Mud-water simultaneous treatment type bottom mud repairing material and preparation method and application thereof |
| CN112960776A (en) * | 2020-12-17 | 2021-06-15 | 云南中贸环境节能科技投资股份有限公司 | Microbial carrier particle for sewage treatment, preparation method and application |
| CN113697923A (en) * | 2021-07-27 | 2021-11-26 | 陵水晨海种业有限公司 | Water quality improver for culture pond and preparation method thereof |
| CN113736770A (en) * | 2021-10-14 | 2021-12-03 | 南京大学 | Granular strain, preparation method thereof and application thereof in biochemical treatment of sewage |
| CN114212897A (en) * | 2021-12-14 | 2022-03-22 | 湖北省水利水电规划勘测设计院 | Lake water environment prediction early warning equipment |
| CN114262128A (en) * | 2021-12-31 | 2022-04-01 | 中国冶金地质总局第二地质勘查院 | Black and odorous inland river treatment by combination of encapsulated microorganisms and composite functional minerals |
| CN114455704A (en) * | 2022-03-01 | 2022-05-10 | 南京大学 | Method for deep biological denitrification enhancement and endocrine disrupting toxicity reduction |
| CN114620832A (en) * | 2022-04-15 | 2022-06-14 | 合肥工业大学 | Sulfur-based autotrophic microbial denitrification material and preparation and application methods thereof |
| CN115010245A (en) * | 2022-05-11 | 2022-09-06 | 安徽理工大学 | Chemical wastewater treating agent, preparation method and application thereof |
| CN115465949A (en) * | 2022-07-29 | 2022-12-13 | 天津正达科技有限责任公司 | Immobilized microorganism composite material with core-shell structure and preparation method thereof |
| CN117623505A (en) * | 2023-10-19 | 2024-03-01 | 北京天诚众合科技发展有限公司 | Sustained-release composite microbial agent for treating black and odorous water body and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107572618A (en) * | 2017-10-30 | 2018-01-12 | 周爱民 | A kind of water purification agent of purifying aquatic product cultivating water environment |
| CN108033575A (en) * | 2017-12-29 | 2018-05-15 | 广州市环境保护工程设计院有限公司 | A kind of black and odorous water remedying sludge method |
-
2018
- 2018-11-01 CN CN201811292667.8A patent/CN109468307A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107572618A (en) * | 2017-10-30 | 2018-01-12 | 周爱民 | A kind of water purification agent of purifying aquatic product cultivating water environment |
| CN108033575A (en) * | 2017-12-29 | 2018-05-15 | 广州市环境保护工程设计院有限公司 | A kind of black and odorous water remedying sludge method |
Non-Patent Citations (2)
| Title |
|---|
| 张启超等: "过氧化钙在处理厌氧底泥中的应用初探", 《湖泊科学》 * |
| 王妙等: "沸石联合过氧化钙对黑臭河道底泥营养盐释放的作用研究", 《广东化工》 * |
Cited By (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110015824A (en) * | 2019-04-11 | 2019-07-16 | 江南大学 | A kind of soft clay microorganism curing agent, the application in soft clay solidifies and application method |
| CN110818202A (en) * | 2019-11-28 | 2020-02-21 | 中冶南方都市环保工程技术股份有限公司 | Method for restoring water ecological system and water ecological system stepped restoration system |
| CN110845024A (en) * | 2019-12-10 | 2020-02-28 | 清华大学 | Underground water remediation simulation experiment system and simulation remediation method |
| CN110937696A (en) * | 2019-12-24 | 2020-03-31 | 河北省科学院生物研究所 | Composite material and method for removing nitrogen and phosphorus in wastewater |
| CN111072132A (en) * | 2020-01-09 | 2020-04-28 | 北京恩菲环保股份有限公司 | Sulfur-iron autotrophic denitrification suspended filler and preparation method thereof |
| CN111484123A (en) * | 2020-05-06 | 2020-08-04 | 北京工业大学 | Efficient nitrifying embedded bioactive filler and preparation method thereof |
| CN111559801A (en) * | 2020-05-06 | 2020-08-21 | 中国城市建设研究院有限公司 | Self-oxygen-release filler for repairing black and odorous water body and preparation method and application thereof |
| CN112604667A (en) * | 2020-11-25 | 2021-04-06 | 南京乐透思环保科技有限公司 | Mud-water simultaneous treatment type bottom mud repairing material and preparation method and application thereof |
| CN112960776A (en) * | 2020-12-17 | 2021-06-15 | 云南中贸环境节能科技投资股份有限公司 | Microbial carrier particle for sewage treatment, preparation method and application |
| CN113697923A (en) * | 2021-07-27 | 2021-11-26 | 陵水晨海种业有限公司 | Water quality improver for culture pond and preparation method thereof |
| CN113736770A (en) * | 2021-10-14 | 2021-12-03 | 南京大学 | Granular strain, preparation method thereof and application thereof in biochemical treatment of sewage |
| CN113736770B (en) * | 2021-10-14 | 2024-03-15 | 南京大学 | Particle strain, preparation method thereof and application thereof in biochemical treatment of sewage |
| CN114212897A (en) * | 2021-12-14 | 2022-03-22 | 湖北省水利水电规划勘测设计院 | Lake water environment prediction early warning equipment |
| CN114212897B (en) * | 2021-12-14 | 2024-08-09 | 湖北省水利水电规划勘测设计院 | Lake water environment prediction early warning equipment |
| CN114262128A (en) * | 2021-12-31 | 2022-04-01 | 中国冶金地质总局第二地质勘查院 | Black and odorous inland river treatment by combination of encapsulated microorganisms and composite functional minerals |
| CN114455704A (en) * | 2022-03-01 | 2022-05-10 | 南京大学 | Method for deep biological denitrification enhancement and endocrine disrupting toxicity reduction |
| CN114620832A (en) * | 2022-04-15 | 2022-06-14 | 合肥工业大学 | Sulfur-based autotrophic microbial denitrification material and preparation and application methods thereof |
| CN114620832B (en) * | 2022-04-15 | 2023-05-02 | 合肥工业大学 | Sulfur-based autotrophic microorganism denitrification material and preparation and application methods thereof |
| CN115010245A (en) * | 2022-05-11 | 2022-09-06 | 安徽理工大学 | Chemical wastewater treating agent, preparation method and application thereof |
| CN115010245B (en) * | 2022-05-11 | 2023-09-12 | 安徽理工大学 | Chemical wastewater treatment agent, preparation method and application thereof |
| CN115465949A (en) * | 2022-07-29 | 2022-12-13 | 天津正达科技有限责任公司 | Immobilized microorganism composite material with core-shell structure and preparation method thereof |
| CN115465949B (en) * | 2022-07-29 | 2024-01-02 | 天津正达科技有限责任公司 | Immobilized microorganism composite material with core-shell structure and preparation method thereof |
| CN117623505A (en) * | 2023-10-19 | 2024-03-01 | 北京天诚众合科技发展有限公司 | Sustained-release composite microbial agent for treating black and odorous water body and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109468307A (en) | Immobilization microorganism particles and preparation method thereof and the method that black and odorous water is efficiently administered with immobilization microorganism particles | |
| CN109534513B (en) | Method for purifying aquaculture wastewater in situ by biochar-enhanced bacteria-algae immobilized preparation | |
| CN108467118B (en) | Method for removing nitrogen and phosphorus in aquaculture wastewater by using immobilized algae bacteria | |
| CN105087541B (en) | Immobilization method of microorganism | |
| CN110697907B (en) | Immobilized composite flora material and preparation method thereof | |
| CN102965365B (en) | Preparation method for microbial nanospheres for water quality purification | |
| CN104261570B (en) | A kind of livestock and poultry cultivation anaerobism purifying agent for polluted water | |
| CN101319212A (en) | Immobilization method for anaerobic fermentation bacterial active sludge | |
| CN110683657A (en) | Culture sewage treatment method based on microbial degradation | |
| CN101691547A (en) | Method for restoring micro-scale polluted reservoir water by using situ bio-contact oxidation | |
| CN109439571A (en) | A kind of ammonia nitrogen removal microbial inoculum | |
| CN106350503A (en) | Preparation method of superior microorganism solid inoculants used for river water pollution management | |
| CN104528947A (en) | Modified bio-enzyme charcoal filler for sewage treatment | |
| CN105695443B (en) | Triton X-100 enhanced biological charcoal immobilized microorganism material and its preparation method and application | |
| CN1928081A (en) | Artificial biological film and preparation method | |
| CN113862191B (en) | Method for removing tetracycline in water by utilizing modified biochar immobilized tetracycline degrading bacteria | |
| CN105176961A (en) | Preparing method of immobilization atrazine degrading bactericide with adsorption and degradation functions | |
| CN114506919A (en) | Efficient purification composite biological filler and preparation method thereof | |
| CN109912136A (en) | A method of utilizing adsorption treatment ammonia nitrogen waste water culture microalgae | |
| CN106754513B (en) | Preparation and application of TX-100 modified sodium alginate embedded pseudomonas particles | |
| CN106698590B (en) | Resin regeneration anaerobic microbial reactor integrating biological culture and biological reduction and application thereof | |
| CN109928517B (en) | Multi-dimensional water body purification system | |
| CN112391375A (en) | Preparation method and application of biochar immobilized denitrifying bacteria for rapidly removing nitrate in water body | |
| CN116622694A (en) | Suspension type immobilized algae-bacteria symbiotic particles and preparation method and application thereof | |
| CN107162168A (en) | A kind of biological aerated filter based on microbial augmentation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190315 |