CN106350503A - Preparation method of superior microorganism solid inoculants used for river water pollution management - Google Patents
Preparation method of superior microorganism solid inoculants used for river water pollution management Download PDFInfo
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- CN106350503A CN106350503A CN201610075670.9A CN201610075670A CN106350503A CN 106350503 A CN106350503 A CN 106350503A CN 201610075670 A CN201610075670 A CN 201610075670A CN 106350503 A CN106350503 A CN 106350503A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
Abstract
The invention relates to the field of river management, in particular to a preparation method of a superior microorganism solid inoculants used for river water pollution management. The preparing method provided by the invention comprises the steps of microbial acclimation, composite bacteria liquid preparation, carrier selection and solid inoculants preparation. On the first aspect, before solid inoculants preparation, microbial acclimation is performed, acclimated goal bacterium in an obtained microorganism mixture has an absolute advantage and is high in content, and microorganisms in the solid inoculants can be in colonization in water, which is help to improve follow-up water pollution management quality and efficiency; on the second aspect, the solid inoculants is prepared by using an absorbing or embedding method, environmental requirements are low, the reaction conditions are mild, the operation is simple, the microorganisms in a composite bacteria liquid can be combined with a carrier tightly, and the biomass concentration in the solid inoculants prepared is high; on the third aspect, the grain size of the carrier is a special ceramsite or molecular sieve support for porous biology, of which the grain size ranges from 1 to 3mm, the intensity ranges from 1.1 to 1.5g/cm<3>, poisonousness is avoided, biocompatibility is excellent, and mass transfer and exchange is facilitated.
Description
Technical field
The present invention relates to field for the treatment of of water pollution is gushed in river, especially relate to a kind of superior microorganism solid fungicide preparation method for river retention of excessive fluid pollution control.
Background technology
Development with social economy, the raising of people's living standard, densely populated bring sanitary sewage, semiworks spreads and brings industrial wastewater, these undressed sanitary sewages, industrial production wastewater discharge unprocessed or that process is not up to standard also increases therewith, continual blowdown for a long time, limit when the self-purification that organic pollution can accommodate beyond river retention of excessive fluid body, dissolved oxygen in water can be consumed rapidly and be in anaerobic condition, river retention of excessive fluid body becomes eutrophication, water body just blacks smelly, due to anoxia, the biological existence of ecosystem is directly on the hazard, until it is dead, the putrefaction of dead body, further polluted-water and stench occurs, so vicious cycle is continuous, the natural ecosystem of rivers is made thoroughly to be destroyed.Additionally, the increasingly destruction of acceleration with town and country construction speed and environmental ecology, most of river is gushed and is stopped or close to cutout so that whole piece river is gushed and become billabong and gush, and water quality deterioration enters vicious cycle.If being not added with strength reason, river is gushed and will is changed into endangering the huge polluter of surrounding, thus having a strong impact on the physical and mental health of people.
Therefore, retention of excessive fluid pollution in river is administered, extremely urgent.At present, river gush administering method mainly have dam, dredge, running water circulation (i.e. diversion flow increasing), river course artificial oxygenation aeration, bed mud covering, coagulation, the method such as restoration of the ecosystem.Though these methods alleviate the problem of river retention of excessive fluid ecological deterioration to a certain extent, there are many weak points.
For example, the pollutant in bed mud in river can be transferred out by dredging work in the short period of time, be a kind of method of sediment pollution of preferably harnessing a river, but cannot solve the emergency purifying of water quality of river；Running water circulation can make the water quality in river be purified in the short period of time by way of diluting and shifting, but the condition required for the method is more special, need to realize the exchange of water using the method for special river level difference or water pump lifting, it is unfavorable for economizing on resources, and the method simply allows pollutant shift to other water bodys, pollutant cannot be removed, cure the symptoms, not the disease；The construction of river aeration and ecological engineering is all to recover and to strengthen the self-purification capacity in river itself as main purpose, gradually recover and purify water, longer time is needed to can be only achieved the effect of purification of water quality, it is suitable as the quick ancillary method improving of coagulation, but for the hydraulics needed for common unhurried current river course difficult to reach coagulation, so that coagulation effect is had a greatly reduced quality；Though coagulation can quickly eliminate " black " of water body, deodorizing effect is not good enough；It is equally a kind of method for governing pollution for bed mud in river that bed mud in river covers, and has the advantages that certain, but still cannot realize water quality of river emergency purifying, and slowly effect is it is impossible to really recover the natural, ecological that river is gushed.
When using microbial treatment water pollution, often need to carry out microorganism solidification, by chemically or physically method, free microorganism is defined in certain area of space so as to keep biological activity.Immobilized microorganism has high mechanical strength, solid-liquid separation easily, can reuse, microorganism concn and active high the advantages of.
Conventional microorganism immobilization method is investment, and the method is it is important that exploitation to carrier material.Common carrier has polyacrylamide, shitosan etc., but monomeric acrylamide has cytotoxicity, preparation process needs heat up, microorganism so can be made to inactivate in a large number in immobilization process, therefore have a strong impact on its application in terms of fixation of microbial cell or immobilized enzyme, shitosan lacking toughness, swelling rear frangible in aqueous, it is not easy to practical application, and become carrier material structure closing, there is no pore passage structure, be unfavorable for that internal microorganism obtains nutrient substance and dissolved oxygen, and the discharge of microbial metabolic products.Both materials to be prepared solid fungicide as carrier, all can affect effect and the efficiency of follow-up water pollution control.
Content of the invention
For solving the above problems, the present invention provides a kind of simple to operate, mild condition, microorganism concn height, carrier and microorganism to be tightly combined, be conducive to improving the effect of follow-up water pollution control and the superior microorganism solid fungicide preparation method for river retention of excessive fluid pollution control of efficiency.
The technical solution adopted in the present invention is: for the superior microorganism solid fungicide preparation method of river retention of excessive fluid pollution control, comprises the following steps:
(1) acclimated microorganism: gather river retention of excessive fluid sample to be administered and bed mud, in bed mud (gram): the ratio mix homogeneously of water sample (ml)=1:2, and in conical flask, vibration 5-15min obtains taming stock solution, will tame stock solution stand for standby use；In the triangular flask of subpackage 100ml enrichment medium and 6 250ml；Domestication stock solution supernatant 10ml is added in first triangular flask, carry out aeration culture at 30 DEG C, often spend 5 days, draw the solution 10ml in triangular flask with aseptic straw to cultivate to another triangular flask, after continuous transferase 45 time, obtain the microorganism mixed culture that enrichment culture purpose bacterium has comparative advantage；
(2) prepare composite bacteria liquid: take the enrichment culture purpose bacterium obtaining in step (1), carry out third stage culture through 10l, 100 l, 1000 l culture tank, obtain composite bacteria liquid；
(3) absorption carrier or occlusion vehicle are selected；
(4) prepare solid fungicide: by the good composite bacteria liquid of the carrier adsorption in step (3) or embedding culture, obtain solid fungicide.
Being improved to further to technique scheme, the absorption carrier in step (3) is multiporous biological special haydite and molecular sieve, the particle diameter 1～3mm of multiporous biological special haydite carrier, density 1.1～1.5g/cm3, porosity be 48～76%, specific surface area be 4 × 104～8 × 104cm2/ g, comprcssive strength are 3～10mpa, shear strength 2～8mpa, moisture content are 0.9-1.0%, wear rate is≤2%, percentage of damage is≤0.03%, hydrochloric acid soluble rate is≤1%, calcination alkali number is≤0.03%, chemical composition is silicon dioxide 60～65%, aluminium sesquioxide 17～21%, iron sesquioxide 7～9%, calcium oxide 3～4%, manganese oxide 1～2%；Described molecular sieve carrier adopts 3a～10a molecular sieve, particle diameter 1～3mm, density 1.1～1.5g/cm3, molecular composition (0～3) cao (0.1～1) na2O (0.2～4.2) al2o3(1.0～6.0) sio2(0～6.5) h2o.
Being improved to further to technique scheme, adsorption method in step (4) is that the carrier in step (3) is totally submerged in the composite bacteria liquid in step (2), is sufficiently mixed, impregnates 2～5 hours, make carrier fully adsorb bacterium solution, remove unnecessary composite bacteria liquid；Obtain solid fungicide after 40 DEG C～70 DEG C low pressure air-dry, then by solid fungicide packed for standby use.
Being improved to further to technique scheme, embedding method in step (4) is, occlusion vehicle in step (3) is totally submerged in cultured composite bacteria liquid, it is sufficiently mixed, dipping 2～5 hours, carrier is made fully to adsorb bacterium solution, filter, filtering residue (carrier of adsorption saturation composite bacteria liquid) and embedding medium is taken to be mixed to prepare mixture, the water adding 0.2～3.5 times of volume of mixture is thoroughly mixed uniformly, solidification was squeezed into shaping after 3～15 hours at a temperature of 30 DEG C～70 DEG C, continue solidification 10～60 hours, or use spray drying granulation machine drying-granulating glomeration or axiolitic solid fungicide at a temperature of 35 DEG C～75 DEG C.
Being improved to further to technique scheme, the carrier filler of the embedding in step (4) is by Calcium Carbonate 65%～85%, glucose 12%～16%, Carnis Bovis seu Bubali cream 5%～8%, kh2po4
3%～9% composition mixed-powder, embedding medium by alginate, polyvinyl, shitosan, gelatin one or more constitute, its consumption be carrier filler 5% ~ 60%.
The invention has the benefit that
1st, on the one hand, before preparing solid fungicide, microorganism is tamed, in the microbial mixture obtaining enrichment culture purpose bacterium have comparative advantage, concentration high, microorganism in solid fungicide can colonize in water body well, can be good at adapting to water body environment to be administered, be conducive to improving quality and the efficiency of follow-up water pollution control；Second aspect, prepares solid fungicide by the method adsorbed or embed, and environmental requirement is not harsh, reaction condition gentle, and simple to operate, and the microorganism in composite bacteria liquid can be tightly combined with carrier, and in prepared solid fungicide, microorganism concn is high；The third aspect, selected carrier is multiporous biological special haydite or molecular sieve carrier, does not have toxicity, and biocompatibility is good, beneficial to mass transfer and exchange, can further improve the microorganism concn in solid fungicide.
2nd, the porosity of multiporous biological special haydite is 48～76%, inside is full of network-like pore passage structure, and surface area is big, and the wear rate of carrier is low, percentage of damage is low, hydrochloric acid soluble rate is low, it is applied to use of large-scale production, be conducive to improving the microorganism concn in solid fungicide and its stability.
Specific embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
For the superior microorganism solid fungicide preparation method of river retention of excessive fluid pollution control, comprise the following steps:
(1) acclimated microorganism: gather river retention of excessive fluid sample to be administered and bed mud, in bed mud (gram): the ratio mix homogeneously of water sample (ml)=1:2, and in conical flask, vibration 10min obtains taming stock solution, will tame stock solution stand for standby use；In the triangular flask of subpackage 100ml enrichment medium and 6 250ml；Domestication stock solution supernatant 10ml is added in first triangular flask, carry out aeration culture at 30 DEG C, often spend 5 days, draw the solution 10ml in triangular flask with aseptic straw to cultivate to another triangular flask, after continuous transferase 45 time, obtain the microorganism mixed culture that enrichment culture purpose bacterium has comparative advantage；
(2) prepare composite bacteria liquid: take the enrichment culture purpose bacterium obtaining in step (1), carry out third stage culture through 10l, 100 l, 1000 l culture tank, obtain composite bacteria liquid；
(3) absorption carrier or occlusion vehicle are selected；
(4) prepare solid fungicide: by the good composite bacteria liquid of the carrier adsorption in step (3) or embedding culture, obtain solid fungicide.
In the present embodiment, the absorption carrier in step (3) is multiporous biological special haydite and molecular sieve, the particle diameter 2mm of multiporous biological special haydite carrier, density 1.3g/cm3, porosity be 60%, specific surface area be 6 × 104cm2/ g, comprcssive strength are 7mpa, shear strength 5mpa, moisture content are 0.95%, wear rate is≤2%, percentage of damage is≤0.03%, hydrochloric acid soluble rate is≤1%, calcination alkali number is≤0.03%, chemical composition is silicon dioxide 60～65%, aluminium sesquioxide 17～21%, iron sesquioxide 7～9%, calcium oxide 3～4%, manganese oxide 1～2%；Described molecular sieve carrier adopts 3a～10a molecular sieve, particle diameter 1～3mm, density 1.1～1.5g/cm3, molecular composition (0～3) cao (0.1～1) na2O (0.2～4.2) al2o3(1.0～6.0) sio2(0～6.5) h2o.
In the present embodiment, the adsorption method in step (4) is that the carrier in step (3) is totally submerged in the composite bacteria liquid in step (2), is sufficiently mixed, impregnates 3 hours, makes carrier fully adsorb bacterium solution, removes unnecessary composite bacteria liquid；Obtain solid fungicide after 55 DEG C of low pressure air-dry, then by solid fungicide packed for standby use.
In the present embodiment; embedding method in step (4) is; occlusion vehicle in step (3) is totally submerged in cultured composite bacteria liquid; it is sufficiently mixed, impregnate 4 hours; carrier is made fully to adsorb bacterium solution; filter; filtering residue (carrier of adsorption saturation composite bacteria liquid) and embedding medium is taken to be mixed to prepare mixture; the water adding 2 times of volume of mixture is thoroughly mixed uniformly; solidification was squeezed into shaping after 9 hours at a temperature of 50 DEG C; continue solidification 40 hours, or use spray drying granulation machine drying-granulating glomeration or axiolitic solid fungicide at a temperature of 55 DEG C.
In the present embodiment, the carrier filler of the embedding in step (4) is by carbonic acid 71%, glucose 14%, Carnis Bovis seu Bubali cream 7%, kh2po4The mixed-powder of 8% composition, embedding medium is made up of alginate, polyvinyl, shitosan and gelatin, and its consumption is the 30% of carrier filler.
The invention has the benefit that
1st, on the one hand, before preparing solid fungicide, microorganism is tamed, in the microbial mixture obtaining enrichment culture purpose bacterium have comparative advantage, concentration high, microorganism in solid fungicide can colonize in water body well, can be good at adapting to water body environment to be administered, be conducive to improving quality and the efficiency of follow-up water pollution control；Second aspect, prepares solid fungicide by the method adsorbed or embed, and environmental requirement is not harsh, reaction condition gentle, and simple to operate, and the microorganism in composite bacteria liquid can be tightly combined with carrier, and in prepared solid fungicide, microorganism concn is high；The third aspect, selected carrier is multiporous biological special haydite or molecular sieve carrier, does not have toxicity, and biocompatibility is good, beneficial to mass transfer and exchange, can further improve the microorganism concn in solid fungicide.
2nd, the porosity of multiporous biological special haydite is 48～76%, inside is full of network-like pore passage structure, and surface area is big, and the wear rate of carrier is low, percentage of damage is low, hydrochloric acid soluble rate is low, it is applied to use of large-scale production, be conducive to improving the microorganism concn in solid fungicide and its stability.
Embodiment described above only have expressed the several embodiments of the present invention, and its description is more concrete and detailed, but therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, some deformation can also be made and improve, these broadly fall into protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be defined by claims.
Claims (5)
1. it is used for the superior microorganism solid fungicide preparation method of river retention of excessive fluid pollution control, comprise the following steps:
(1) acclimated microorganism: gather river retention of excessive fluid sample to be administered and bed mud, in bed mud (gram): the ratio mix homogeneously of water sample (ml)=1:2, and in conical flask, vibration 5-15min obtains taming stock solution, will tame stock solution stand for standby use；In the triangular flask of subpackage 100ml enrichment medium and 6 250ml；Domestication stock solution supernatant 10ml is added in first triangular flask, carry out aeration culture at 30 DEG C, often spend 5 days, draw the solution 10ml in triangular flask with aseptic straw to cultivate to another triangular flask, after continuous transferase 45 time, obtain the microorganism mixed culture that enrichment culture purpose bacterium has comparative advantage；
(2) prepare composite bacteria liquid: take the enrichment culture purpose bacterium obtaining in step (1), carry out third stage culture through 10l, 100 l, 1000 l culture tank, obtain composite bacteria liquid；
(3) absorption carrier or occlusion vehicle are selected；
(4) prepare solid fungicide: by the good composite bacteria liquid of the carrier adsorption in step (3) or embedding culture, obtain solid fungicide.
2. the superior microorganism solid fungicide preparation method for river retention of excessive fluid pollution control according to claim 1, it is characterized in that: the absorption carrier in described step (3) is multiporous biological special haydite and molecular sieve, particle diameter 1～the 3mm of multiporous biological special haydite carrier, density 1.1～1.5g/cm3, porosity be 48～76%, specific surface area be 4 × 104～8 × 104cm2/ g, comprcssive strength are 3～10mpa, shear strength 2～8mpa, moisture content are 0.9-1.0%, wear rate is≤2%, percentage of damage is≤0.03%, hydrochloric acid soluble rate is≤1%, calcination alkali number is≤0.03%, chemical composition is silicon dioxide 60～65%, aluminium sesquioxide 17～21%, iron sesquioxide 7～9%, calcium oxide 3～4%, manganese oxide 1～2%；Described molecular sieve carrier adopts 3a～10a molecular sieve, particle diameter 1～3mm, density 1.1～1.5g/cm3, molecular composition (0～3) cao (0.1～1) na2O (0.2～4.2) al2o3(1.0～6.0) sio2(0～6.5) h2o.
3. the superior microorganism solid fungicide preparation method for river retention of excessive fluid pollution control according to claim 2, it is characterized in that: the adsorption method in step (4) is, carrier in step (3) is totally submerged in the composite bacteria liquid in step (2), it is sufficiently mixed, impregnate 2～5 hours, make carrier fully adsorb bacterium solution, remove unnecessary composite bacteria liquid；Obtain solid fungicide after 40 DEG C～70 DEG C low pressure air-dry, then by solid fungicide packed for standby use.
4. the superior microorganism solid fungicide preparation method for river retention of excessive fluid pollution control according to claim 3, it is characterized in that: the embedding method in step (4) is, occlusion vehicle in step (3) is totally submerged in cultured composite bacteria liquid, it is sufficiently mixed, dipping 2～5 hours, carrier is made fully to adsorb bacterium solution, filter, filtering residue (carrier of adsorption saturation composite bacteria liquid) and embedding medium is taken to be mixed to prepare mixture, the water adding 0.2～3.5 times of volume of mixture is thoroughly mixed uniformly, solidification was squeezed into shaping after 3～15 hours at a temperature of 30 DEG C～70 DEG C, continue solidification 10～60 hours, or use spray drying granulation machine drying-granulating glomeration or axiolitic solid fungicide at a temperature of 35 DEG C～75 DEG C.
5. the superior microorganism solid fungicide preparation method for river retention of excessive fluid pollution control according to claim 4 it is characterised in that: in step (4) embedding carrier filler be by Calcium Carbonate 65%～85%, glucose 12%～16%, Carnis Bovis seu Bubali cream 5%～8%, kh2po4
3%～9% composition mixed-powder, embedding medium by alginate, polyvinyl, shitosan, gelatin one or more constitute, its consumption be carrier filler 5% ~ 60%.
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| CN108017161A (en) * | 2017-12-25 | 2018-05-11 | 陈益德 | A kind of superior microorganism solid fungicide preparation method for creek water pollution control |
| CN108060095A (en) * | 2017-10-23 | 2018-05-22 | 东莞松山湖高新技术产业开发区循环经济发展促进中心东莞生态产业园区循环经济发展促进中心 | A kind of control method of microbiological contamination and application |
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| CN110092526A (en) * | 2019-03-29 | 2019-08-06 | 卢松 | For handling the biological agent of xanthan gum fermenting process waste water |
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| CN114716013A (en) * | 2022-05-07 | 2022-07-08 | 红菌生物(广东)有限公司 | Method for inoculating anaerobic ammonium oxidation bacteria |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108060095A (en) * | 2017-10-23 | 2018-05-22 | 东莞松山湖高新技术产业开发区循环经济发展促进中心东莞生态产业园区循环经济发展促进中心 | A kind of control method of microbiological contamination and application |
| CN108866109A (en) * | 2017-11-21 | 2018-11-23 | 河南理工大学 | A method of improving coal biology gas production rate |
| CN108017161A (en) * | 2017-12-25 | 2018-05-11 | 陈益德 | A kind of superior microorganism solid fungicide preparation method for creek water pollution control |
| CN110092526A (en) * | 2019-03-29 | 2019-08-06 | 卢松 | For handling the biological agent of xanthan gum fermenting process waste water |
| CN110092527A (en) * | 2019-03-29 | 2019-08-06 | 卢松 | A kind of environment-protective process handling xanthan gum fermentation waste water |
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| CN110877950A (en) * | 2019-11-13 | 2020-03-13 | 佛山科学技术学院 | Water body pollution treatment method based on microbial precipitation technology |
| CN114716013A (en) * | 2022-05-07 | 2022-07-08 | 红菌生物(广东)有限公司 | Method for inoculating anaerobic ammonium oxidation bacteria |
| CN114716013B (en) * | 2022-05-07 | 2023-06-16 | 红菌生物(广东)有限公司 | Method for inoculating anaerobic ammonia oxidizing bacteria |
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