CN109897794B - Biological activated carbon cultured by mixed wastewater and taking fern leaves as carbon source carrier - Google Patents
Biological activated carbon cultured by mixed wastewater and taking fern leaves as carbon source carrier Download PDFInfo
- Publication number
- CN109897794B CN109897794B CN201810985728.2A CN201810985728A CN109897794B CN 109897794 B CN109897794 B CN 109897794B CN 201810985728 A CN201810985728 A CN 201810985728A CN 109897794 B CN109897794 B CN 109897794B
- Authority
- CN
- China
- Prior art keywords
- carbon
- wastewater
- mixed
- mixed wastewater
- activated carbon
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Abstract
The invention belongs to the technical field of biological water purifying agents, and particularly relates to biological activated carbon cultured by mixed wastewater and taking fern leaves as a carbon source carrier. The preparation method comprises the following steps: placing hydrophilic porous carbon material powder in a mixed wastewater culture medium, inoculating a mixed strain to the mixed wastewater culture medium in two steps, and performing oscillation co-culture and drying to obtain biological activated carbon; the hydrophilic porous carbon material powder is prepared by taking fern leaves as a raw material and carrying out hydrothermal synthesis, calcination in a nitrogen atmosphere and crushing; the mixed wastewater culture medium contains biogas slurry wastewater and high-sugar wastewater. The hydrophilic carbon carrier has better intermiscibility with water, so that the adsorption efficiency of the hydrophilic carbon carrier on pollutants is improved, the bacterial carbon water purifying agent is easy to precipitate, and the bacterial carbon water purifying agent has double purification functions of carbon physical adsorption and microbial enzyme degradation.
Description
Technical Field
The invention belongs to the technical field of biological water purifying agents, and particularly relates to biological activated carbon cultured by mixed wastewater and taking fern leaves as a carbon source carrier.
Background
In the development of economy, polluted water sources, air and soil bring more and more serious influence on the life of people. Researchers are systematically studying treatment methods and processes of various kinds of sewage when treating wastewater caused in industrial, agricultural (production and processing) and aquaculture production, and respectively utilize chemical methods and biological methods to adsorb and separate nitrogen, phosphorus, heavy metal ions and organic matter molecules of the sewage and carry out biological/chemical degradation. In the test and application process, the activated carbon is usually used as an adsorbent for sewage pollutants, the chromaticity and turbidity of the sewage can be improved, and the activated carbon also has adsorption effect on micromolecules and heavy metal ions due to the macroporous, mesoporous and microporous structures; the bacterial carbon water purifying agent can be used as a carrier of a microbial water purifying agent to prepare the bacterial carbon water purifying agent, but the sewage purifying effect of the bacterial carbon water purifying agent formed by fixing a single bacterial strain on a carbon carrier is not ideal as that of the bacterial carbon water purifying agent formed by mixing the bacterial strains.
The publication No. CN 104261570A discloses an anaerobic sewage purifying agent for livestock and poultry breeding, which consists of 5 microbial strains and an activated carbon carrier, wherein the activated carbon carrier is coal columnar activated carbon which is solid columnar activated carbon prepared by shaping coal activated carbon of 2-10 meshes. The coal-based activated carbon and commercially available carbon materials (activated carbon AC, carbon nanotube CNT, graphite GC and the like) have the advantages of high porosity, large specific surface, good stability, no toxicity, no pollution, environmental friendliness and the like; but the carbon material has fewer surface functional groups and does not contain hydrophilic groups (-COOH, -OH), so the purifying agent has poor dispersibility in water, prevents the full contact with pollutants in sewage, hardly generates electrostatic interaction with nitrogen-containing substances containing hydrogen bonds and lone electron pairs, and cannot completely exert the adsorption effect of carbon-based carriers on the pollutants, thereby the carbon material is very necessary to regulate and control the surface hydrophilicity/hydrophobicity. The method commonly used at present is to use concentrated acid (concentrated HNO) 3 Concentrated H 2 SO 4 ) The inert carbon surface is activated and oxidized to form oxygen-containing functional groups (-COOH, -OH, -C = O), and simultaneously, the polluted nitrogen oxides and sulfur oxides are generated, thereby causing secondary pollution.
The patent application with publication number CN 103949216A discloses a preparation method of a carbon material for adsorbing hydrophilic ionic liquid, which takes cellulose as a raw material and carries out hydrothermal reaction in a high-pressure hydrothermal reaction kettle to obtain carbon microspheres rich in oxygen-containing groups such as hydroxyl, carboxyl and the like. Incomplete hydrothermal carbonization of cellulose can yield carbon microspheres rich in oxygen-containing groups on the surface.
Publication number CN 106531471A discloses a carbon material for super capacitor electrode material and a preparation method thereof, comprising the following steps: s101: putting a biomass raw material into a reaction kettle for hydrothermal treatment (the temperature of the hydrothermal treatment is 150-300 ℃ and the time is 6-48 h) to obtain hydrogel; s102: sequentially soaking the hydrogel in ethanol water and water to remove impurities to obtain the hydrogel after impurity removal; s103: drying the hydrogel after impurity removal to obtain aerogel; s104: and (2) calcining the aerogel in the atmosphere of nitrogen or rare gas (the calcining temperature is 800-1200 ℃, and the calcining time is 2-5 h) to obtain the carbon material.
The publication numbers CN 107473404A, CN 107434305A and CN 107512780A all disclose bacterial carbon water purifying agents, a carbon carrier containing a hydrophilic group is placed in a culture medium, and simultaneously a mixed strain is inoculated to the culture medium and is subjected to shaking co-culture to prepare the bacterial carbon water purifying agent. However, when the culture medium is shake co-cultured, the culture medium is beef extract peptone medium or Luria-Bertani medium. Compared with the culture method adopting mixed wastewater, the bacterial carbon water purifying agent obtained by culturing the beef extract peptone culture medium or the Luria-Bertani culture medium needs an adaptation period of 2-3d and has a service life of about 24d-28d in practical application.
The pteridophyte leaves are leaves of pteridophyte, mainly comprise alkaloid (alkali containing carboxyl with bioactivity and optical activity), phenolic compound (polyhydroxy oxygen-containing group), brass compound (containing oxygen-containing substituent such as hydroxyl, methoxyl, oxyl, isopentenyloxyl and the like) and steroid and triterpenoid compound (containing hydroxyl, carbonyl and carboxyl groups), are plant leaves with abundant oxygen-containing groups in the structure, and the oxygen-containing functional groups of part of the plant bodies reserved after hydrothermal reaction and calcination treatment at 166-200 ℃ can improve the hydrophilicity of the carbon surface and the interaction with the electrical groups of pollutants, thereby being beneficial to improving the adsorption efficiency of organic pollutants in mixed wastewater.
Disclosure of Invention
The invention aims to provide biological activated carbon which is cultured by mixed wastewater and takes fern leaves as a carbon source carrier, aiming at the defects of the prior art. The water purifying agent prepared by the invention has high microbial activity and good water purifying effect; the method has the effect of dual-cycle purification of carbon physical adsorption and enzyme catalytic degradation, realizes the resource utilization of organic matters in biogas slurry wastewater and high-sugar wastewater by microorganisms, simultaneously takes natural agricultural and forestry wastes as a carbon source, and applies the synthesized material to pollutant treatment, thereby realizing the resource utilization of the agricultural and forestry wastes and the dual-pollution treatment of the wastes and sewage.
In order to realize the purpose of the invention, the following technical scheme is adopted:
a preparation method of biological activated carbon cultured by mixed wastewater and taking fern leaves as a carbon source carrier comprises the following steps: placing hydrophilic porous carbon material powder in mixed wastewater culture medium, and adding Trichoderma reesei (Trichoderma reesei) (III)Trichoderma reesei) Candida utilis (A), (B)Candida utilis) Bacillus subtilis preparation (B)Bacillus subtilis) Rhodococcus erythropolis (A), (B), (C)Rhodococcus erythropolis) Inoculating the strain to a mixed wastewater culture medium, performing shake co-culture, and inoculating Bacillus subtilis (Bacillus subtilis)Bacillus subtilis) Planktonic coccobacillus (A) and (B)Sphaerotilus natans) Bacillus cereus (B.cereus)Bacillus cereus) Actinoplanes flavus (A), (B)Actinoplanes flavus) Inoculating into a mixed wastewater culture medium, performing oscillation co-culture, and drying to obtain biological activated carbon; the mixed wastewater culture medium contains biogas slurry wastewater and high-sugar wastewater.
The formula of the mixed wastewater culture medium is as follows: naCl 2.0 g, biogas slurry waste water 0.6L and high sugar waste water 0.4L, and pH is 7.0-7.2.
The preparation method of the mixed wastewater culture medium comprises the following steps: the high-sugar wastewater is independently 0.56kg/cm 2 Sterilizing at 112.6 deg.C for 20min; after NaCl is added into the biogas slurry wastewater, the concentration is 1.05 kg/cm 2 Sterilizing at 121.0 deg.C for 30min;after respective sterilization, the high-sugar wastewater is added into the biogas slurry wastewater in proportion, and the pH is adjusted to 7.2 +/-0.2.
The indexes of the biogas slurry wastewater are as follows: COD 2000-4000mg/L, BOD 1000-2500 mg/L, SS 50-200 mg/L, ammonia nitrogen 600-800 mg/L, total phosphorus 30-80 mg/L, and pH 7.0-8.0; the indexes of the high-sugar wastewater are as follows: COD 3000-5000mg/L, BOD-3000 mg/L, SS 600-800 mg/L, ammonia nitrogen about 100 mg/L, pH is 4.0-6.0.
The hydrophilic porous carbon material powder is prepared by taking fern leaves as a raw material through hydrothermal synthesis, calcination in a nitrogen atmosphere and crushing.
The preparation method of the hydrophilic porous carbon material powder comprises the following specific steps:
(1) Placing fern leaf powder with the granularity of 50.00 g being 0.1-0.3 cm in an inner container of a 200 ml reaction kettle, adding 150ml of deionized water, reacting at the temperature of 160-200 ℃ for 8-20 hours, and generating a carbon precursor material;
(2) Washing the carbon precursor material prepared in the step (1) with water and absolute ethyl alcohol in sequence, and drying at 60 ℃ for 12 hours to obtain a carbon precursor powder sample;
(3) And (3) calcining the carbon precursor powder sample prepared in the step (2) for 2 hours at 600 ℃ in a nitrogen atmosphere to obtain hydrophilic porous carbon material powder.
The preparation method of the biological activated carbon cultured by the mixed wastewater and taking the fern leaves as the carbon source carrier comprises the following specific steps:
1) Adding 100g of hydrophilic porous carbon material powder into 50-150 ml mixed wastewater culture medium, and adding Trichoderma reesei (Trichoderma reesei)) (Trichoderma reesei) Candida utilis (A), (B)Candida utilis) Bacillus subtilis preparation (B)Bacillus subtilis) Rhodococcus erythropolis (A), (B), (C)Rhodococcus erythropolis) The activated bacterium solution is mixed according to the mass ratio of 3;
2) Then Bacillus subtilis (B.subtilis) is addedBacillus subtilis) Planktonic coccobacillus (A) and (B)Sphaerotilus natans) Bacillus cereus (B.cereus)Bacillus cereus) Actinoplanes flavus (A), (B)Actinoplanes flavus) The activated bacteria liquid is mixed according to the mass ratio of 4.
A biological activated carbon prepared by the preparation method as described above: the effective viable count on the biological activated carbon is 8.2 multiplied by 10 8 ～1.3×10 9 CFU/g。
The application of the biological activated carbon in purifying mixed wastewater is characterized in that the volume ratio of the biogas slurry wastewater to the high-sugar wastewater in the mixed wastewater is 3:2.
The species used in the above raw materials may be those which are conveniently isolated from nature by conventional species identification and isolation methods by those skilled in the art, or those which are publicly available through commercial channels. The strain adopted by the invention can be purchased from bacterial seed libraries such as the China general microbiological collection center, the Guangdong province microbiological collection center and the like.
The strains are cultured according to a slant strain preservation culture medium:
1) Trichoderma reesei medium: sucrose 30.0g, naNO 3 2.0g、K 2 HPO 4 1.0g、MgSO 4 ·H 2 O 0.5g、KCl 0.5g、FeSO 4 ·7H 2 0.5g of O, 15-20g of agar and 1000mL of distilled water, and the pH value is natural.
2) Candida utilis culture medium: 1.0g of glucose, 1.8g of KCl, 2.5g of yeast extract, 8.2g of sodium acetate, 15-20g of agar and 1000mL of distilled water, and the pH value is natural. Dissolving, packaging into test tubes, and sterilizing with damp heat at 115 deg.C for 20min.
3) Culture medium of bacillus subtilis, rhodococcus erythropolis and bacillus cereus: 10.0g of tryptone, 5.0g of yeast extract, 10.0g of NaCl, 15-20g of agar and 1000mL of distilled water, and the pH value is 7.0-7.2.
4) Culture medium of sphaerotheca plankton and actinoplanes flavus: sucrose 30.0g, naNO 3 2.0g、K 2 HPO 4 ·3H 2 O 1.0g、KCl 0.5g、MgSO 4 ·7H 2 O 0.5g、FeSO 4 ·7H 2 0.01g of O, 15-20g of agar and 1000mL of distilled water, and the pH value is 7.0-7.2.
The invention has the beneficial effects that:
1) The invention takes agricultural and forestry waste, namely fern leaves, as a raw material, prepares the hydrophilic porous carbon carrier through hydrothermal synthesis and low-temperature calcination, avoids the defect that toxic polluting gas is generated when the carbon is etched by using concentrated acid in the conventional method, and the charged groups on the surface of the carbon carrier and H in water + /OH - The electrostatic interaction is generated, so that the carbon carrier has better dispersibility in water, the problem of hydrophobicity of a common carbon carrier is solved, the contact probability with pollutants is increased, and meanwhile, the charged groups on the carbon surface of the carrier can generate electrostatic interaction with lone-pair electrons and hydrogen protons of pollutants, so that the adsorption efficiency of the pollutants is improved; the material synthesized by taking the agricultural and forestry wastes as the carbon source is applied to pollutant treatment, so that the resource utilization of the agricultural and forestry wastes and the double pollution treatment of the wastes and sewage are realized;
2) The invention cultures functional microorganism strains which are beneficial to the early stage and can degrade saccharides and celluloses which are not easy to decompose by two steps, fully utilizes substances such as oligosaccharide, cellulose, protein and a small part of fat in mixed wastewater, decomposes the substances into micromolecular substances such as monosaccharide, fatty acid, organic nitrogen, fatty acid and the like by enzymolysis, is beneficial to the later stage and adds functional microorganism strains which can degrade organic matters, ammonia nitrogen, total phosphorus and the like in water body to grow and reproduce, improves the effective viable count in the bacterial liquid, enhances the biological activity of the microorganism, promotes the microorganism to efficiently degrade pollutants, and removes most of the pollutants to achieve the purpose of sewage purification;
3) According to the invention, the biological activated carbon is cultured by adopting the mixed wastewater, and the microbial strains can take the pollutants in the mixed wastewater as nutrient substrates for growth and propagation of the microbial strains, so that most of organic pollutants in the sewage are removed, and the resource utilization of the biogas slurry wastewater and the high-sugar wastewater by the microorganisms is realized; the products of microbial metabolism can be used as nutrient substrates for mutual utilization, and the biological activity of the microbes is improved to promote the synergistic degradation effect of the microbes on pollutants; the biological activated carbon is cultured by adopting the mixed wastewater, so that the adaptability of microbial strains is improved, the purification effect can be effectively exerted in the actual wastewater purification application, and the service life is long;
4) The bacterial carbon water purifying agent has double purifying effects of carbon adsorption and microbial degradation, has stable decontamination effect and easy treatment, and is suitable for large-scale treatment of mixed wastewater (mixed liquid of biogas slurry and high-sugar wastewater).
Detailed Description
For further disclosure, but not limitation, the present invention is described in further detail below with reference to examples.
A biological activated carbon taking fern leaves cultured by mixed wastewater as a carbon source carrier comprises the following specific contents:
1. preparing a hydrophilic porous carbon carrier with fern leaves as a carbon source:
(1) Placing 50.00 g fern leaf powder (the granularity is 0.1-0.3 cm) into a liner of a 200 ml reaction kettle, adding 150ml of deionized water, reacting at 160-200 ℃ for 8-20 hours to generate a carbon precursor material;
(2) Washing the carbon precursor material with water and absolute ethyl alcohol in sequence, and drying at 60 ℃ for 12h to obtain a carbon precursor powder sample;
(3) And (3) calcining the carbon precursor powder sample in a nitrogen atmosphere at 600 ℃ for 2h to obtain the hydrophilic porous carbon powder carrier.
2. Shaking co-culture:
1) One-step culture: adding 100g of hydrophilic porous carbon material powder into 50-150 ml mixed wastewater culture medium, and adding Trichoderma reesei (Trichoderma reesei)) (Trichoderma reesei) Candida utilis (A), (B)Candida utilis) Bacillus subtilis preparation (B)Bacillus subtilis) Rhodococcus erythropolis (A), (B), (C)Rhodococcus erythropolis) The activated bacterium solution is mixed according to the mass ratio of 3;
2) Two-step culture: then Bacillus subtilis (B.subtilis) is addedBacillus subtilis) Planktonic coccobacillus (A) and (B)Sphaerotilus natans) Bacillus cereus (Bacillus cereus) ((Bacillus cereus))Bacillus cereus) Actinoplanes flavus (A), (B)Actinoplanes flavus) The activated bacteria liquid is mixed according to the mass ratio of 4.
The number of effective viable bacteria on the carbon carrier is 8.2 multiplied by 10 8 ～1.3×10 9 CFU/g。
The bacterial carbon water purifying agent preserved at 4 ℃ is placed at normal temperature for 8-12 hours before use, and can be directly put into sewage after biological activity is recovered.
Example 1
A biological activated carbon taking fern leaves cultured by mixed wastewater as a carbon source carrier comprises the following specific contents:
(1) Preparing a hydrophilic porous carbon carrier with fern leaves as a carbon source:
1) Placing 50.00 g fern leaf powder (the granularity is 0.15 cm) into a liner of a 200 ml reaction kettle, adding 150ml of deionized water, reacting at 180 ℃ for 12 hours to generate a carbon precursor material;
2) Washing the carbon precursor material with water and absolute ethyl alcohol in sequence, and drying at 60 ℃ for 12 hours to obtain a carbon precursor powder sample;
3) And (3) calcining the carbon precursor powder sample in a nitrogen atmosphere at 600 ℃ for 2h to obtain the hydrophilic porous carbon powder carrier.
2. Adding 10g of hydrophilic porous carbon carrier into 100 ml mixed wastewater culture medium, and adding Trichoderma reesei (Trichoderma reesei) into the culture mediumTrichoderma reesei) Candida utilis (A), (B)Candida utilis) Bacillus subtilis preparation (B)Bacillus subtilis) Rhodococcus erythropolis (A), (B), (C)Rhodococcus erythropolis) The activated bacterium solution of (2) is mixed according to the mass ratio of 3Bacillus subtilis) Plankton coccolium（Sphaerotilus natans) Bacillus cereus (Bacillus cereus) ((Bacillus cereus))Bacillus cereus) Actinoplanes flavus (A), (B)Actinoplanes flavus) The activated bacterium liquid is mixed according to the mass ratio of 4; the number of effective viable bacteria on the carbon carrier is 1.1 × 10 9 ～1.3×10 9 CFU/g; the bacterial carbon water purifying agent stored at 4 ℃ is placed at normal temperature for 10 hours before use to recover the biological activity, and then the bacterial carbon water purifying agent can be directly put into sewage.
Example 2
A biological activated carbon taking fern leaves cultured by mixed wastewater as a carbon source carrier comprises the following specific contents:
(1) Preparing a hydrophilic porous carbon carrier with fern leaves as a carbon source:
1) Placing 50.00 g fern leaf powder (the granularity is 0.15 cm) into a liner of a 200 ml reaction kettle, adding 150ml of deionized water, reacting at 160 ℃ for 8 hours to generate a carbon precursor material;
2) Washing the carbon precursor material with water and absolute ethyl alcohol in sequence, and drying at 60 ℃ for 12h to obtain a carbon precursor powder sample;
3) And (3) calcining the carbon precursor powder sample in a nitrogen atmosphere at 600 ℃ for 2h to obtain the hydrophilic porous carbon powder carrier.
2. Adding 10g of hydrophilic porous carbon carrier into 50ml mixed wastewater culture medium, and adding Trichoderma reesei (Trichoderma reesei)Trichoderma reesei) Candida utilis (A), (B)Candida utilis) Bacillus subtilis preparation (B)Bacillus subtilis) Rhodococcus erythropolis (A), (B)Rhodococcus erythropolis) The activated bacterium solution of (2) is mixed according to a mass ratio of 3Bacillus subtilis) Planktonic coccobacillus (A) and (B)Sphaerotilus natans) Bacillus cereus (B.cereus)Bacillus cereus) Actinoplanes flavus (A), (B)Actinoplanes flavus) Is/are as followsThe activated bacteria liquid is mixed according to the mass ratio of 4; the effective viable count on the carbon carrier is 9.6 multiplied by 10 8 ～1.2×10 9 CFU/g; the bacterial carbon water purifying agent preserved at 4 ℃ is placed at normal temperature for 8 hours before use to recover the biological activity, and then the bacterial carbon water purifying agent can be directly put into sewage.
Example 3
A biological activated carbon taking fern leaves cultured by mixed wastewater as a carbon source carrier comprises the following specific contents:
(1) Preparing a hydrophilic porous carbon carrier with fern leaves as a carbon source:
1) Placing 50.00 g fern leaf powder (the granularity is 0.15 cm) into a 200 ml reaction kettle inner container, adding 150ml deionized water, reacting at 200 ℃ for 20 hours to generate a carbon precursor material;
2) Washing the carbon precursor material with water and absolute ethyl alcohol in sequence, and drying at 60 ℃ for 12h to obtain a carbon precursor powder sample;
3) And (3) calcining the carbon precursor powder sample in a nitrogen atmosphere at 600 ℃ for 2h to obtain the hydrophilic porous carbon powder carrier.
2. Adding 10g of hydrophilic porous carbon carrier into 150ml mixed wastewater culture medium, and adding Trichoderma reesei (Trichoderma reesei)Trichoderma reesei) Candida utilis (A), (B)Candida utilis) Bacillus subtilis preparation (B)Bacillus subtilis) Rhodococcus erythropolis (A), (B), (C)Rhodococcus erythropolis) The activated bacterium solution of (2) is mixed according to a mass ratio of 3Bacillus subtilis) Planktonic coccobacillus (A) and (B)Sphaerotilus natans) Bacillus cereus (B.cereus)Bacillus cereus) Actinoplanes flava: (A), (B)Actinoplanes flavus) The activated bacterium solution is mixed according to the mass ratio of 4,forming mixed bacteria liquid, finally precipitating out carbon carriers, and placing the carbon carriers in a constant temperature box at 20-30 ℃ for drying to obtain a water purifying agent; the number of effective viable bacteria on the carbon carrier is 8.2 multiplied by 10 8 ～1.1×10 9 The bacterial carbon water purifying agent stored at the temperature of 4 ℃ in the CFU/g is placed at the normal temperature for 12 hours before use to recover the biological activity, and then the bacterial carbon water purifying agent can be directly put into sewage.
Comparative example 1
1. Essentially the same as in example 1, except that the carbon support was replaced with a 100g commercially available granular coal-based activated carbon support (specific surface area about 1100 m) 2 Per g, pore volume of 0.75 cm 3 /g）。
Performance testing of water purifying agent:
1. different materials carbon carrier and this patent biological activity carbon carrier on sale contrast pollutant adsorption efficiency
Different materials of activated carbon carriers are put into the mixed wastewater (the volume ratio of the biogas slurry to the high-sugar wastewater is 3:2) to compare the adsorption effects. Respectively weighing 100g of activated carbon carriers (granular coal activated carbon A, granular coconut shell activated carbon B, granular bamboo carbon C and powdered carbon D in example 1) of various materials, adding into 10L of mixed wastewater, and measuring CODcr and BOD of untreated mixed wastewater after 72h 5 And calculating the adsorption rate of the activated carbon to each index in the mixed wastewater.
The results are shown in Table 1-1. The experimental results show that the granular coal-based activated carbon in the three materials has better adsorption capacity on the mixed wastewater, and COD and BOD of the granular coal-based activated carbon 5 The adsorption rates of ammonia nitrogen, total phosphorus and the like are higher than those of the former two; the adsorption effect of the powdered carbon is better than that of A, B, C, the adsorption rate is improved, and the surface modification is favorable for adsorption removal of pollutants.
TABLE 1-1 treatment effect of carbon carriers of different shapes on mixed wastewater
2. Stain removal Performance test
Five mixed wastewater tanks (10L) with the same size and water quality are taken for testing, and the biological activated carbon of the embodiment 1 is put in the first tank; secondly, the bacterial carbon water purifying agent of the embodiment 2 is added; thirdly, the bacterial carbon water purifying agent of the embodiment 3 is added; fourthly, the bacterial carbon water purifying agent in the comparative example 1 is added; the fifth, without any water purifying agent, served as a blank control group.
100g water purifying agent is respectively put into a mixed wastewater tank (water quality index: COD 3278mg/L, BOD 1337mg/L, ammonia nitrogen 379mg/L and total phosphorus 45 mg/L), and after 72 hours, water quality determination is carried out on all the wastewater tanks. The water quality detection standard refers to the discharge standard of water pollutants in sugar industry (GB 21909-2008) and the discharge standard of pollutants in livestock and poultry breeding industry (GB 18596-2001), and the determination indexes and data are shown in the following tables 1-2 and 1-3.
The effect of different purifiers on the index concentration of sewage in the time of tables 1-2 72h
The effect of different purifiers on the removal of contaminants in tables 1-3 72h
As can be seen from the data in the table, the water quality indexes of the examples 1 to 3 are all superior to those of the comparative example 1, and are more obviously superior to those of a blank control group.
Comparative example 2
The service life of the carbon material of the biological activated carbon cultured by the beef extract peptone culture medium is about 28 days, while the service life of the biological activated carbon cultured by the mixed wastewater is about 36 days, which is improved by about 28.6 percent.
Comparative example 3
The mixed wastewater culture adopts a step-by-step method, which is favorable for improving the effective viable count of microorganisms and improving the biological activity of the microorganisms, and the effective viable count of the microorganisms loaded on the activated carbon after the first step of culture is 8.3 multiplied by 10 7 ～1.2×10 8 CFU/g, effective viable count of microorganism loaded on activated carbon after two-step culture is 8.2 × 10 9 ～1.1×10 10 CFU/g. Respectively putting the 100g water purifying agent into the mixed wastewater after the one-step culture and the two-step culture, and measuring the water quality after 72 hours of treatment, wherein the measuring indexes and data are shown in the following tables 1-4.
TABLE 1-4 treatment effect of biological activated carbon obtained by stepwise culture on sewage
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.
Claims (6)
1. A preparation method of biological activated carbon cultured by mixed wastewater and taking fern leaves as a carbon source carrier is characterized by comprising the following steps: placing hydrophilic porous carbon material powder in mixed wastewater culture medium, and adding Trichoderma reesei (Trichoderma reesei) (III)Trichoderma reesei) Candida utilis (A), (B)Candida utilis) Bacillus subtilis preparation (B)Bacillus subtilis) Rhodococcus erythropolis (A), (B), (C)Rhodococcus erythropolis) Inoculating the strain to a mixed wastewater culture medium, performing shake co-culture, and inoculating Bacillus subtilis (Bacillus subtilis)Bacillus subtilis) Planktonic coccobacillus (A) and (B)Sphaerotilus natans) Bacillus cereus (Bacillus cereus) ((Bacillus cereus))Bacillus cereus) Actinoplanes flavus (A), (B)Actinoplanes flavus) Inoculating into a mixed wastewater culture medium, performing oscillation co-culture, and drying to obtain biological activated carbon;
the mixed wastewater culture medium contains biogas slurry wastewater and high-sugar wastewater; the formula of the mixed wastewater culture medium is as follows: naCl 2.0 g, biogas slurry waste water 0.6L and high sugar waste water 0.4L, pH 7.2 +/-0.2; the hydrophilic porous carbon material powder is prepared by taking fern leaves as a raw material and carrying out hydrothermal synthesis, calcination in a nitrogen atmosphere and crushing;
the preparation method of the biological activated carbon cultured by the mixed wastewater and taking the fern leaves as the carbon source carrier comprises the following specific steps:
1) Adding 100g hydrophilic porous carbon material powder into 50-150 ml mixed wastewater culture medium, and mixing Trichoderma reesei (Trichoderma reesei)Trichoderma reesei) Candida utilis (A), (B)Candida utilis) Bacillus subtilis preparation (B)Bacillus subtilis) Rhodococcus erythropolis (A), (B)Rhodococcus erythropolis) The activated bacterium solution is mixed according to the mass ratio of 3;
2) Then Bacillus subtilis (B) is addedBacillus subtilis) Planktonic coccobacillus (A. Planktonic.), (Sphaerotilus natans) Bacillus cereus (B.cereus)Bacillus cereus) Actinoplanes flavus (A), (B)Actinoplanes flavus) The activated bacteria liquid is mixed according to the mass ratio of 4.
2. The method for preparing biological activated carbon which is cultured by mixed wastewater and takes fern leaves as a carbon source carrier according to claim 1, is characterized in that: the preparation method of the culture medium comprises the following steps: the high-sugar wastewater is independently 0.56kg/cm 2 Sterilizing at 112.6 deg.C for 20min; after NaCl is added into the biogas slurry wastewater, the concentration is 1.05 kg/cm 2 Sterilizing at 121.0 deg.C for 30min; after respective sterilization, the high-sugar wastewater is added into the biogas slurry wastewater in proportion, and the pH is adjusted to 7.2 +/-0.2.
3. The method for preparing biological activated carbon using fern leaves as a carbon source carrier cultured by mixed wastewater according to claim 1, wherein the method comprises the following steps: the indexes of the biogas slurry wastewater are as follows: COD 2000-4000mg/L, BOD 1000-2500 mg/L, suspended Solid (Suspended Solid) 50-200 mg/L, ammonia nitrogen 600-800 mg/L, total phosphorus 30-80 mg/L, and pH 7.0-8.0; the indexes of the high-sugar wastewater are as follows: COD 3000-5000mg/L, BOD-3000 mg/L, suspended Solid (Suspended Solid) 600-800 mg/L, ammonia nitrogen 100 +/-5 mg/L, pH is 4.0-6.0.
4. The method for preparing biological activated carbon which is cultured by mixed wastewater and takes fern leaves as a carbon source carrier according to claim 1, is characterized in that: the preparation method of the hydrophilic porous carbon material powder comprises the following specific steps:
(1) Placing fern leaf powder with the particle size of 50.00 g of 0.1-0.3 cm into an inner container of a 200 ml reaction kettle, adding 150ml of deionized water, reacting at the temperature of 160-200 ℃ for 8-20 hours, and generating a carbon precursor material;
(2) Washing the carbon precursor material prepared in the step (1) with water and absolute ethyl alcohol in sequence, and drying at 60 ℃ for 12h to obtain a carbon precursor powder sample;
(3) And (3) calcining the carbon precursor powder sample prepared in the step (2) for 2 hours at 600 ℃ in a nitrogen atmosphere to obtain hydrophilic porous carbon material powder.
5. A biological activated carbon produced by the production method according to claim 1, characterized in that: the effective viable count on the biological activated carbon is 8.2 multiplied by 10 8 ～1.3×10 9 CFU/g。
6. Use of the biological activated carbon of claim 5 in mixed wastewater purification, characterized in that: the volume ratio of the biogas slurry wastewater to the high-sugar wastewater in the mixed wastewater is 3:2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810985728.2A CN109897794B (en) | 2018-08-28 | 2018-08-28 | Biological activated carbon cultured by mixed wastewater and taking fern leaves as carbon source carrier |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810985728.2A CN109897794B (en) | 2018-08-28 | 2018-08-28 | Biological activated carbon cultured by mixed wastewater and taking fern leaves as carbon source carrier |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109897794A CN109897794A (en) | 2019-06-18 |
| CN109897794B true CN109897794B (en) | 2022-11-15 |
Family
ID=66943232
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810985728.2A Active CN109897794B (en) | 2018-08-28 | 2018-08-28 | Biological activated carbon cultured by mixed wastewater and taking fern leaves as carbon source carrier |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109897794B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110817841A (en) * | 2019-12-20 | 2020-02-21 | 北京理工大学 | Method for preparing hierarchical pore doped carbon material by microorganisms |
| CN111498987A (en) * | 2020-04-16 | 2020-08-07 | 南开大学 | Domestic sewage in-situ purification immobilized carrier and microbial inoculum loading method |
| CN113716705A (en) * | 2021-09-01 | 2021-11-30 | 英丽(中山)生物科技有限公司 | Natural plant extract composition capable of improving microbial degradation capability and application thereof |
| CN117165496B (en) * | 2023-11-02 | 2024-02-09 | 同济大学 | Efficient culture method of phosphate-solubilizing bacteria and application of phosphate-solubilizing bacteria in repairing heavy metal contaminated sites by combining carbon microspheres |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174582A (en) * | 2011-03-07 | 2011-09-07 | 井冈山大学 | Carrier-free immobilized culture method of microbial flocculating agent |
| CN102719482A (en) * | 2012-02-28 | 2012-10-10 | 湖南大学 | Method for producing microbial flocculant by utilizing culture wastewater and residual activated sludge |
| CN103642729A (en) * | 2013-12-03 | 2014-03-19 | 湖北工业大学 | Method for producing Bacillus subtilis for feeds by fermenting high-salt-content amino acid wastewater |
| CN107434305A (en) * | 2017-09-29 | 2017-12-05 | 福建省农业科学院农业工程技术研究所 | A kind of water purification agent of rich defect carbon carrier fixation of microbe and preparation method thereof |
| CN107512780A (en) * | 2017-09-29 | 2017-12-26 | 福建省农业科学院农业工程技术研究所 | A kind of bacterium carbon water purification agent for removing aquaculture wastewater middle and high concentration ammonia nitrogen and preparation method thereof |
| CN108046401A (en) * | 2017-11-22 | 2018-05-18 | 闽南师范大学 | The method for preparing activated carbon nanometer iron composite material using autumn eggplant leaf |
-
2018
- 2018-08-28 CN CN201810985728.2A patent/CN109897794B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174582A (en) * | 2011-03-07 | 2011-09-07 | 井冈山大学 | Carrier-free immobilized culture method of microbial flocculating agent |
| CN102719482A (en) * | 2012-02-28 | 2012-10-10 | 湖南大学 | Method for producing microbial flocculant by utilizing culture wastewater and residual activated sludge |
| CN103642729A (en) * | 2013-12-03 | 2014-03-19 | 湖北工业大学 | Method for producing Bacillus subtilis for feeds by fermenting high-salt-content amino acid wastewater |
| CN107434305A (en) * | 2017-09-29 | 2017-12-05 | 福建省农业科学院农业工程技术研究所 | A kind of water purification agent of rich defect carbon carrier fixation of microbe and preparation method thereof |
| CN107512780A (en) * | 2017-09-29 | 2017-12-26 | 福建省农业科学院农业工程技术研究所 | A kind of bacterium carbon water purification agent for removing aquaculture wastewater middle and high concentration ammonia nitrogen and preparation method thereof |
| CN108046401A (en) * | 2017-11-22 | 2018-05-18 | 闽南师范大学 | The method for preparing activated carbon nanometer iron composite material using autumn eggplant leaf |
Non-Patent Citations (2)
| Title |
|---|
| 竹炭-微生物联合法对沼液中COD的去除;王莉玮等;《西南师范大学学报(自然科学版)》;20121020(第10期);全文 * |
| 臭氧生物活性炭工艺处理某多金属硫化矿浮选废水的小试研究;王自超等;《环境工程学报》;20130505(第05期);全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109897794A (en) | 2019-06-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109897794B (en) | Biological activated carbon cultured by mixed wastewater and taking fern leaves as carbon source carrier | |
| CN107434305B (en) | Water purifying agent with defect-rich carbon carrier immobilized microorganisms and preparation method thereof | |
| CN112960781B (en) | Organic pollutant degradation method based on biological nanometer heterozygous system | |
| CN107473404B (en) | Water purifying agent with self-formed block-shaped carbon carrier for fixing microorganisms and preparation method thereof | |
| CN102443550B (en) | Screening method of denitrifying bacteria | |
| CN113562846B (en) | Denitrification efficient carbon source and processing technology thereof | |
| CN110981082B (en) | Purification process of threonine fermentation wastewater | |
| CN109897869B (en) | Biological activated carbon cultured by high-sugar wastewater and using ficus microcarpa leaves as carbon source carrier | |
| CN108585179B (en) | Compound microbial preparation for water pollution treatment and preparation method thereof | |
| CN114410695A (en) | Hydroxyapatite, preparation method thereof and application thereof in hydrogen production by dark fermentation | |
| Hu et al. | Enhanced sulfamethoxazole degradation in soil by immobilized sulfamethoxazole-degrading microbes on bagasse | |
| CN116333938A (en) | Marine bacteria and application thereof in preparation of biological nano selenium | |
| CN109896703B (en) | Light-enzyme composite catalytic function microorganism water purifying agent for culturing anaerobic sewage | |
| CN107512780B (en) | Bacterial carbon water purifying agent for removing high-concentration ammonia nitrogen in aquaculture sewage and preparation method thereof | |
| Xia et al. | Simultaneous adsorption and biodegradation of oxytetracycline in wastewater by Mycolicibacterium sp. immobilized on magnetic biochar | |
| CN116004459A (en) | Rhodococcus YZ-1 and application thereof in degrading organic pollutants | |
| CN112266074B (en) | Method for enhancing denitrification of heterotrophic nitrification-aerobic denitrification strain by magnesium salt modified biomass charcoal | |
| CN112694997A (en) | Compound microorganism bacterium stock solution suitable for sewage treatment and active solution culture method | |
| CN107384829B (en) | Application of bacillus megatherium YJB3 in artificial wetland water PAEs pollution purification | |
| CN110818180A (en) | Method for digesting black and odorous river channel by using microbial preparation | |
| CN110937694A (en) | Biochemical preparation for treating amino acid fermentation wastewater | |
| CN115558684B (en) | Biological preparation method of amide compound and acrylamide prepared by biological method | |
| CN115612641B (en) | Triazolone degrading bacterial agent and preparation method thereof | |
| CN117089502B (en) | Immobilized methane-oxidizing bacteria and immobilization method and application thereof | |
| CN114958686B (en) | Quinone-based modified biochar-based microbial agent and preparation and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |