CN107189971A - House refuse and cultivation fecal deodorizing microbial bacterial agent, Preparation method and use - Google Patents
House refuse and cultivation fecal deodorizing microbial bacterial agent, Preparation method and use Download PDFInfo
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Abstract
The present invention provides a kind of house refuse and cultivation fecal deodorizing microbial bacterial agent, Preparation method and use, and microbial bacterial agent includes following component：Saccharomyces cerevisiae, lactobacillus acidophilus, bafillus natto, bacillus subtilis and white-rot fungi.The weight proportion of each component is：The parts by weight of saccharomyces cerevisiae 30~70；The parts by weight of lactobacillus acidophilus 10~60；The parts by weight of bafillus natto 5~30；The parts by weight of bacillus subtilis 5~20；The parts by weight of white-rot fungi 10~30.Advantage is：Strain has the ability for suppressing corrupt pathogen growth breeding, and the deodorization to stink substance can be played from source.Microbial inoculum has living bacteria count amount high, and the dosage of microbial inoculum can be reduced by handling same amount of material, so as to be greatly reduced operating cost.
Description
Technical field
The invention belongs to technical field of environmental microorganism, and in particular to a kind of house refuse and the cultivation micro- life of fecal deodorizing
Thing microbial inoculum, Preparation method and use.
Background technology
As the improvement of people's living standards, the house refuse produced is more and more, the content of organic matter in house refuse
Year by year into ascendant trend, in big city at home, the house refuse content of organic matter alreadys exceed 60%, and house refuse is being received
The odorous gas such as ammonia, hydrogen sulfide, the physical and mental health of serious infringement people, pollution environment are produced during fortune, transfer.With people
Living standard improve that match is that breeding enterprise is more and more, the animal wastes that breeding enterprise is produced cannot be rationally effective
Processing so that cultivation place stink to high heaven, pollute surrounding enviroment.
The stink produced for corrupt house refuse and cultivated animals excreta, also has scientific worker to develop some changes
The deodorising product of work class and plant.These products achieve certain effect in terms of house refuse and fecal deodorizing, still
It is not enough below generally existing：(1) first, these products are to lean in some chemical reagents to add essence, and simply stink substance is risen
Certain masking action has been arrived, the generation (such as ammonia, hydrogen sulfide) of stink substance is not eliminated from source.(2) secondly, chemical industry
Class product, has certain toxicity to environment the characteristics of due to raw material itself, there is also the potential risk of secondary pollution environment.
(3) again, it is the plant extract class raw material for having used hypotoxicity both to have made, expensive there is also raw material, the high shortcoming of production cost.
The content of the invention
The defect existed for prior art, the present invention provides a kind of house refuse and cultivation fecal deodorizing microbial bacteria
Agent, Preparation method and use, can effectively solve the above problems.
The technical solution adopted by the present invention is as follows：
The present invention provides a kind of house refuse and cultivation fecal deodorizing microbial bacterial agent, including following component：Wine brewing ferment
Mother, lactobacillus acidophilus, bafillus natto, bacillus subtilis and white-rot fungi.
It is preferred that, the weight proportion of each component is：
It is preferred that, the weight proportion of each component is：
It is preferred that, total effective viable bacteria content is 4 × 10 in every gram of microbial bacterial agent8~3 × 109CFU, and it is every kind of
The number of viable of single bacterium is not less than 1 × 108CFU。
The present invention also provides a kind of house refuse and cultivates the preparation method of fecal deodorizing microbial bacterial agent, including following
Step：
Step 1, the preparation of first order seed liquid：
The saccharomyces cerevisiae after activation, lactobacillus acidophilus, bafillus natto and bacillus subtilis are inoculated in LB respectively
In fluid nutrient medium, cultivate 2~3 days, until seed maturity, be prepared into respectively under conditions of 100~400rpm, 28~45 DEG C
To saccharomyces cerevisiae first order seed, lactobacillus acidophilus first order seed, bafillus natto first order seed and bacillus subtilis one-level
Seed；
White-rot fungi after activation is inoculated in PDA liquid medium, in 100~400rpm, 28~45 DEG C of condition
Lower culture 3~5 days, until seed maturity, prepares white-rot fungi first order seed；
Step 2, deodorant fluid microbial inoculum amount mixed fungus fermentation：
The saccharomyces cerevisiae first order seed that is obtained for step 1, lactobacillus acidophilus first order seed, bafillus natto one-level kind
Son, bacillus subtilis first order seed and white-rot fungi first order seed, by the parts by weight of saccharomyces cerevisiae first order seed 30~70, acidophilus
The parts by weight of lactobacillus first order seed 10~60, the parts by weight of bafillus natto first order seed 5~30, bacillus subtilis one-level kind
The ratio inoculum concentration of sub 5~20 parts by weight and the parts by weight of white-rot fungi first order seed 10~30 is inoculated into liquid fermentation tank culture medium
Middle fermentation；Wherein, fermentation is：28~45 DEG C of temperature, throughput control be 0.5~2L/min, speed of agitator 300~
500rpm, coefficient control is 75%, and pressure 0.1MPa ferments 3~5 days, prepares liquid deodorant microbial inoculum.
It is preferred that, in step 2, liquid fermentation tank culture medium composition is：The parts by weight of corn flour 2~5, the weight of glucose 1~2
Part, the parts by weight of corn steep liquor 5~10, remaining is water.
It is preferred that, before step 1, in addition to：
The preparation of initial strain：Respectively from household refuse landfill sites, plant's animal wastes collecting sampling, by sample nothing
Bacterium water is diluted after 50 times, in shaking 1~2h under conditions of 200 revs/min on concussion bed, then using mass fraction as 5%
Inoculum concentration is respectively connected to cultivate 5 days in LB culture mediums and PDA culture medium, and so repeatedly 5 times, pregnant solution then is diluted into 108
~1010Times, on the flat board for being coated on LB and PDA selectivity, select the typical single bacterium colony of form and carry out test tube slant preservation, and
Censorship 16S RNA are identified, five plants of deodorization strains are screened altogether, are respectively：Saccharomyces cerevisiae, lactobacillus acidophilus, bacillus subtilis,
Bafillus natto and white-rot fungi；
Activation：The strain of 4 DEG C of preservations is subjected to slant activation, i.e.,：Respectively by saccharomyces cerevisiae, lactobacillus acidophilus, natto bud
Spore bacillus, bacillus subtilis are inoculated in LB inclined-planes, are activated 2 days under conditions of 35 DEG C, saccharomyces cerevisiae after being activated, thermophilic
Lactobacillus lactis, bafillus natto, bacillus subtilis；White-rot fungi is inoculated in PDA inclined-planes, activated under conditions of 37 DEG C
3 days, the white-rot fungi after being activated.
The present invention also provides a kind of house refuse and cultivates the purposes of fecal deodorizing microbial bacterial agent, and microbial bacterial agent is used
In house refuse and cultivation fecal deodorizing, its deodorisation process is：Microbial bacterial agent is put into house refuse and excrement, micro- life
Saccharomyces cerevisiae and lactobacillus acidophilus in thing microbial inoculum suppress corrupt class bacterial reproduction in house refuse and excrement, while can remove
Ammonia and hydrogen sulfide gas in house refuse and excrement；Bafillus natto and producing bacillus subtilis life neutral proteinase,
Protease can decompose the organic nitrogen of the protein-based generation stink of excrement class, and be translated into nitrate nitrogen, reach deodorization；
The material that white rot fungus degrading lignin hardly possible is decomposed, while organic sulfur odorous gas of degrading.
Strain properties and function of the present invention are as follows：
1) saccharomyces cerevisiae：It can decompose and produce ethanol using starch and sugar-metabolism.
2) lactobacillus acidophilus：Generation lactic acid can be metabolized, the inhibitory action to putrefactivebacteria can be played, while can go
The ammonia produced except house refuse and stink substance due to anaerobic fermentation.
3) bacillus subtilis：Protease production is high, can effectively decompose the albumen in excrement and house refuse, soon
Fast degradation of ammonia nitrogen.
4) white-rot fungi：The hydrogen sulfide gas and all kinds of organic sulfurs in house refuse can be effectively removed, is removed so as to play
Smelly effect.
Between house refuse and cultivation fecal deodorizing microbial bacterial agent that the present invention is provided, contained each microbial bacteria
With good synergy.Under the synergy, the organic substance in excrement and house refuse can be effectively decomposed, to corruption
Bacterium plays inhibitory action, so as to play a part of deodorization.Specifically, bacillus subtilis is decomposed in excrement and house refuse
Albumen, produces protease, for the nutriment needed for other microbial bacterias provide propagation；Saccharomyces cerevisiae and lactobacillus acidophilus difference
Metabolism produces ethanol and lactic acid, reaches the inhibitory action to putrefactivebacteria, at the same can remove house refuse and stink substance by
The ammonia produced in anaerobic fermentation.Hydrogen sulfide gas and all kinds of organic sulfurs in white-rot fungi removing house refuse, so as to play
The effect of deodorization.
The house refuse and cultivation fecal deodorizing microbial bacterial agent, Preparation method and use that the present invention is provided have following
Advantage：
1) technique uses multiple bacteria compound fermentation technique, greatly simplify production technology, reduces being produced into for microbial inoculum
This；
2) microbial inoculum has living bacteria count amount high, and the effective viable bacteria of every gram of product reaches as high as 4 × 108-3×109CFU, because
This, the dosage of microbial inoculum can be reduced by handling same amount of material, so as to be greatly reduced operating cost；
3) strain is used to be screened respectively from house refuse and animal wastes, adaptable, the suitable life of product
Rubbish, sewage plant sludge and plant's animal wastes deodorization.
4) fast growth of strain, odor removal efficient is high, can be played in 24h and the obvious deodorization of stink substance is made
With.
5) strain selected by has the ability for suppressing corrupt pathogen growth breeding, can be played from source to stink substance
Deodorization.
Brief description of the drawings
House refuse and the flow of the preparation method of cultivation fecal deodorizing microbial bacterial agent that Fig. 1 provides for the present invention
Figure.
Embodiment
In order that technical problem solved by the invention, technical scheme and beneficial effect are more clearly understood, below in conjunction with
Drawings and Examples, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only used to
The present invention is explained, is not intended to limit the present invention.
The present invention provides a kind of house refuse and cultivation fecal deodorizing microbial bacterial agent, Preparation method and use, this hair
The bright seed selection from house refuse and excrement is to can quickly suppress in house refuse and excrement the wine brewing of corrupt class bacterial reproduction
Yeast and lactobacillus acidophilus, while the ammonia and hydrogen sulfide gas in house refuse and excrement can be removed effectively.From excrement
The bafillus natto screened in class material and bacillus subtilis can produce substantial amounts of neutral proteinase, and protease can
The organic nitrogen of the effective protein-based generation stink for decomposing excrement class, and nitrate nitrogen is translated into, quickly removed so as to play
Smelly material.The white-rot fungi screened from municipal garden garbage can effectively lignin degrading etc. hardly possible decompose material,
Can also degrade the odorous gas such as organic sulfur simultaneously.
The microbial bacterial agent strong adaptability of the present invention, deodorizing capability is strong, efficiency high, is adapted to house refuse, plant animal
The deodorization of excrement, sludge etc..Can be with decomposition smell material more than 90% ammonia nitrogen, more than 80% hydrogen sulfide etc. in 48h
Odorous gas.
House refuse and cultivation fecal deodorizing microbial bacterial agent that the present invention is provided, including following component：Saccharomyces cerevisiae,
Lactobacillus acidophilus, bafillus natto, bacillus subtilis and white-rot fungi.The weight proportion of each component is：Saccharomyces cerevisiae 30
~70 parts by weight；The parts by weight of lactobacillus acidophilus 10~60；The parts by weight of bafillus natto 5~30；The weight of bacillus subtilis 5~20
Measure part；The parts by weight of white-rot fungi 10~30.For microbial bacterial agent, total effective viable bacteria content in every gram of microbial bacterial agent
4 × 108~3 × 109CFU, and the number of viable of every kind of single bacterium is not less than 1 × 108CFU。
House refuse and the preparation method for cultivating fecal deodorizing microbial bacterial agent, comprise the following steps：
Step 1, the preparation of first order seed liquid：
The saccharomyces cerevisiae after activation, lactobacillus acidophilus, bafillus natto and bacillus subtilis are inoculated in LB respectively
In fluid nutrient medium, cultivate 2~3 days, until seed maturity, be prepared into respectively under conditions of 100~400rpm, 28~45 DEG C
To saccharomyces cerevisiae first order seed, lactobacillus acidophilus first order seed, bafillus natto first order seed and bacillus subtilis one-level
Seed；
White-rot fungi after activation is inoculated in PDA liquid medium, in 100~400rpm, 28~45 DEG C of condition
Lower culture 3~5 days, until seed maturity, prepares white-rot fungi first order seed；
Step 2, deodorant fluid microbial inoculum amount mixed fungus fermentation：
The saccharomyces cerevisiae first order seed that is obtained for step 1, lactobacillus acidophilus first order seed, bafillus natto one-level kind
Son, bacillus subtilis first order seed and white-rot fungi first order seed, by the parts by weight of saccharomyces cerevisiae first order seed 30~70, acidophilus
The parts by weight of lactobacillus first order seed 10~60, the parts by weight of bafillus natto first order seed 5~30, bacillus subtilis one-level kind
The ratio inoculum concentration of sub 5~20 parts by weight and the parts by weight of white-rot fungi first order seed 10~30 is inoculated into liquid fermentation tank culture medium
Middle fermentation；Wherein, fermentation is：28~45 DEG C of temperature, throughput control be 0.5~2L/min, speed of agitator 300~
500rpm, coefficient control is 75%, and pressure 0.1MPa ferments 3~5 days, prepares liquid deodorant microbial inoculum.
The present invention is further elaborated by the following examples
Following four culture medium is prepared respectively, it is standby.
First, test tube slant culture medium (PDA culture medium and LB culture mediums)
PDA culture medium：The potato 150g of skin is removed, is cut into small pieces, is filtered after addition 1L boiling tap waters 1h, collects filter
Liquid addition 15g glucose is settled to 1L, adjusts pH to 6.5, and addition 20g agar, which is boiled to agar, to be melted, and is sub-packed in test tube, is gone out
Bacterium, prepares test tube slant culture medium.
LB culture mediums：Peptone 10g, beef extract 3g, sodium chloride 5g and water 1000ml are taken, addition 20g agar is boiled to fine jade
Fat melts, and is sub-packed in test tube, sterilizes, prepares test tube slant culture medium.
2nd, liquid primary-seed medium：Formula, without agar, thus obtains PDA liquid with test tube slant culture medium
Culture medium and LB fluid nutrient mediums.
3rd, liquid fermentation tank culture medium：The parts by weight of corn flour 2~5, the parts by weight of glucose 1~2, the weight of corn steep liquor 5~10
Part, remaining is water, is sterilized standby.
Embodiment 1
1st step：The preparation of initial strain
Respectively from household refuse landfill sites, plant's animal wastes collecting sampling, sample is diluted after 50 times with sterilized water,
In shaking 1~2h under conditions of 200RPM (rev/min) on concussion bed, then distinguish by 5% inoculum concentration of mass fraction
Access in LB culture mediums and PDA culture medium and cultivate 5 days, so repeatedly 5 times, pregnant solution is then diluted to 108~1010Times, apply
On the flat board for being distributed in LB and PDA selectivity, select the typical single bacterium colony of form and carry out test tube slant preservation, and censorship 16S
RNA is identified.This laboratory screens five plants of preferable strains of deodorizing effect of A, B, C, D, E altogether, identified, and A is saccharomyces cerevisiae, B
It is that bacillus subtilis, D are that bafillus natto, E are white-rot fungi for lactobacillus acidophilus, C.
2nd step：Activation
The strain of 4 DEG C of preservations is subjected to slant activation：Respectively by saccharomyces cerevisiae, lactobacillus acidophilus, bafillus natto, withered
Careless bacillus is inoculated in LB inclined-planes, is activated 2 days under conditions of 35 DEG C, saccharomyces cerevisiae, lactobacillus acidophilus after being activated,
Bafillus natto, bacillus subtilis.White-rot fungi is inoculated in PDA inclined-planes, activates 3 days, obtains under conditions of 37 DEG C
White-rot fungi after activation.
3rd step：The preparation of first order seed
Respectively from the saccharomyces cerevisiae after picking activation on the test tube slant of activation, lactobacillus acidophilus, bafillus natto, withered
Careless bacillus, is inoculated in LB fluid nutrient mediums, is cultivated 3 days under conditions of 35 DEG C, 200rpm, until seed maturity, point
Saccharomyces cerevisiae first order seed, lactobacillus acidophilus first order seed, bafillus natto first order seed and withered grass gemma are not prepared
Bacillus first order seed；
White-rot fungi after activation is inoculated in PDA liquid medium, cultivated 5 days under conditions of 37 DEG C, 200rpm,
Until seed maturity, white-rot fungi first order seed is prepared；
4th step：Fermented by mixed bacterium prepares liquid deodorizing microorganism product：
Parts by weight of saccharomyces cerevisiae first order seed 70 that 3rd step is obtained, the parts by weight of lactobacillus acidophilus first order seed 10, natto
The parts by weight of bacillus first order seed 5, the parts by weight of bacillus subtilis first order seed 5 and the parts by weight of white-rot fungi first order seed 10
Ratio inoculum concentration be inoculated into the culture medium of liquid fermentation tank ferment；Wherein, fermentation is：35 DEG C of temperature, ventilation
Amount control is 2L/min, and speed of agitator 300rpm, coefficient control is 75%, and pressure 0.1MPa ferments 4 days, prepares liquid
Body deodorizing microorganism.
After testing, effective viable bacteria content is 3 × 10 in every gram of microbial bacterial agent for the liquid deodorant microbial inoculum obtained9CFU。
Proteinase activity is 200U/g.
Embodiment 2
1st step：The preparation of initial strain
Respectively from household refuse landfill sites, plant's animal wastes collecting sampling, sample is diluted after 50 times with sterilized water,
In shaking 1~2h under conditions of 200RPM (rev/min) on concussion bed, then distinguish by 5% inoculum concentration of mass fraction
Access in LB culture mediums and PDA culture medium and cultivate 5 days, so repeatedly 5 times, pregnant solution is then diluted to 108~1010Times, apply
On the flat board for being distributed in LB and PDA selectivity, select the typical single bacterium colony of form and carry out test tube slant preservation, and censorship 16S
RNA is identified.This laboratory screens five plants of preferable strains of deodorizing effect of A, B, C, D, E altogether, identified, and A is saccharomyces cerevisiae, B
It is that bacillus subtilis, D are that bafillus natto, E are white-rot fungi for lactobacillus acidophilus, C.
2nd step：Activation
The strain of 4 DEG C of preservations is subjected to slant activation：Respectively by saccharomyces cerevisiae, lactobacillus acidophilus, bafillus natto, withered
Careless bacillus is inoculated in LB inclined-planes, is activated 2 days under conditions of 35 DEG C, saccharomyces cerevisiae, lactobacillus acidophilus after being activated,
Bafillus natto, bacillus subtilis.White-rot fungi is inoculated in PDA inclined-planes, activates 3 days, obtains under conditions of 37 DEG C
White-rot fungi after activation.
3rd step：The preparation of first order seed
Respectively from the saccharomyces cerevisiae after picking activation on the test tube slant of activation, lactobacillus acidophilus, bafillus natto, withered
Careless bacillus, is inoculated in LB fluid nutrient mediums, is cultivated 2 days under conditions of 45 DEG C, 400rpm, until seed maturity, point
Saccharomyces cerevisiae first order seed, lactobacillus acidophilus first order seed, bafillus natto first order seed and withered grass gemma are not prepared
Bacillus first order seed；
White-rot fungi after activation is inoculated in PDA liquid medium, cultivated 3 days under conditions of 28 DEG C, 400rpm,
Until seed maturity, white-rot fungi first order seed is prepared；
4th step：Fermented by mixed bacterium prepares liquid deodorizing microorganism product：
Parts by weight of saccharomyces cerevisiae first order seed 30 that 3rd step is obtained, the parts by weight of lactobacillus acidophilus first order seed 40, natto
The parts by weight of bacillus first order seed 30, the parts by weight of bacillus subtilis first order seed 15 and the weight of white-rot fungi first order seed 30
The ratio inoculum concentration of part, which is inoculated into the culture medium of liquid fermentation tank, ferments；Wherein, fermentation is：Temperature 45 C, leads to
Tolerance control is 0.5L/min, and speed of agitator 500rpm, coefficient control is 75%, and pressure 0.1MPa ferments 5 days, is prepared into
To liquid deodorant microbial inoculum.
After testing, effective viable bacteria content is 3 × 10 in every gram of microbial bacterial agent for the liquid deodorant microbial inoculum obtained9CFU。
Proteinase activity is 200U/g.
Inspection example 1
This inspection example is used for the deodorizing capability for investigating microbial bacterial agent.
Corrupt house refuse and chicken manure are taken, according to 1:1 ratio mixing, then by mixture and water according to 1：1.5 mixing
Filtered after uniform, collect filtrate and used to do source of odor.
Configure basal medium：Peptone 10g, beef extract 3g, sodium chloride 5g, water 1000ml, are sub-packed in 10 500ml's
It is standby after being sterilized in triangular flask.
Source of odor solution is added into 10ml into each triangular flask, each strain 10ml is then respectively connected to, 30 DEG C,
The deodorizing capability for detecting each bacterium after 48h with odor threshold method is cultivated under conditions of 150rpm.
Experimental method：1ml zymotic fluids are taken in small beaker, deionization is then added with each 10ml amount into small beaker
Water, until can't smell stink, records diluted multiple, the preliminary judgment criteria screened as deodorization bacterium.Experimental result is as follows
Shown in table.
Saccharomyces cerevisiae, lactobacillus acidophilus, bacillus subtilis and the white rot screened it can be seen from data above is true
Bacterium to the degradation rate of stink substance all more than 80%, and 5 kinds of bacterium the order of magnitude equal percentage mixing after stink substance is gone
Except rate is up to 89.7%.
Inspection example 2
This inspection example is used for the drop ammonia effect for investigating microbial bacterial agent.
Ammonia experiment is dropped：20ml bacteria liquids are added in 1L beaker, 5ml5% ammoniacal liquor is added, is then placed in 100ml's
Absorbing liquid equipped with 10ml kjeldahl determinations, after being sealed with plastic sheeting, after static gas wave refrigerator 48h under conditions of 30 DEG C, takes out small
Beaker, is titrated, the experimental results are shown inthe following table using 0.05mol/L sulfuric acid standard liquid.
It can be seen from data above saccharomyces cerevisiae, lactobacillus acidophilus, white-rot fungi drop ammonia effect preferably, all exist
More than 80%, saccharomyces cerevisiae performance is protruded the most, has reached 93.4%.The mixing of 5 kinds of bacterium reaches to the degradation rate of ammonia
93.85%.
Inspection example 3
This inspection example is used for the desulfurized effect for investigating microbial bacterial agent.
Desulfurization：
The sodium sulfide solution for configuring 0.015g/L is standby.
Bacteria liquid 20ml is added in 500ml beaker, 2ml 0.015g/L sodium sulfide solution is then added, then
The 50ml small beakers equipped with 20ml zinc ammion complex salt absorbing liquids are put into large beaker, with being put into 30 DEG C of cultures after plastic film sealing
In case, the concentration of hydrogen sulfide in sampling detection absorbing liquid after 24 and 48h is cultivated respectively.
The detection method of concentration of hydrogen sulfide：5ml absorbing liquids are taken in colorimetric cylinder, 0.2ml diformazan is then added thereto
Base para-phenylene diamine dihydrochloride developer, 20min is after colour developing under 665nm wavelength.Using the burst size of water control group hydrogen sulfide as
100% calculates the percentage contents of hydrogen sulfide reduction, and the experimental results are shown inthe following table.
The compound Mixed Microbes prepared of 5 kinds of bacterium are up to 86% to the clearance of hydrogen sulfide it can be seen from data above,
Therefore, deodorizing effect is more satisfactory.
Inspection example 4
This inspection example is used for the deodorizing effect for investigating microbial bacterial agent.
Household garbage field processing experiment：
The microbial bacterial agent that the embodiment of the present invention 1 is prepared is transported to Dezhou household refuse landfill sites and carries out deodorization effect
Fruit qualification test.
Specifically, by ten times of microbial bacteria dilution agent, life is sprayed onto by bacteria liquid is diluted by the amount of house refuse 10%
On rubbish, while using water as control, determining stink grade with sensory evaluation method in 12,24,36,48,60,72h respectively, as a result
It is as shown in the table：
| 0h | 12 | 24 | 36 | 48 | 60 | 72 | |
| Microbial inoculum group | ++++ | +++ | +++ | ++ | ++ | + | + |
| Water is compareed | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ |
Note：++++represent strong stench +++ represent denseer ++ represent that stink is relatively light+represent to be destitute of smell substantially
As can be seen from the above table, using the microbial bacterial agent for preparing of the present invention, deodorizing effect is just shown in 12h, 2~
The purpose for removing house refuse stink can substantially be reached within 3 days.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
Depending on protection scope of the present invention.
Claims (8)
1. a kind of house refuse and cultivation fecal deodorizing microbial bacterial agent, it is characterised in that including following component：Wine brewing ferment
Mother, lactobacillus acidophilus, bafillus natto, bacillus subtilis and white-rot fungi.
2. house refuse according to claim 1 and cultivation fecal deodorizing microbial bacterial agent, it is characterised in that each component
Weight proportion be：
3. house refuse according to claim 2 and cultivation fecal deodorizing microbial bacterial agent, it is characterised in that each component
Weight proportion be：
4. house refuse according to claim 1 and cultivation fecal deodorizing microbial bacterial agent, it is characterised in that every gram of institute
Effective viable bacteria content total in microbial bacterial agent is stated 4 × 108~3 × 109CFU, and the number of viable of every kind of single bacterium is not less than 1
×108CFU。
5. a kind of house refuse and the preparation method for cultivating fecal deodorizing microbial bacterial agent, it is characterised in that including following step
Suddenly：
Step 1, the preparation of first order seed liquid：
The saccharomyces cerevisiae after activation, lactobacillus acidophilus, bafillus natto and bacillus subtilis are inoculated in LB liquid respectively
In culture medium, cultivated 2~3 days under conditions of 100~400rpm, 28~45 DEG C, until seed maturity, wine is prepared respectively
Brewer yeast first order seed, lactobacillus acidophilus first order seed, bafillus natto first order seed and bacillus subtilis first order seed；
White-rot fungi after activation is inoculated in PDA liquid medium, trained under conditions of 100~400rpm, 28~45 DEG C
Support 3~5 days, until seed maturity, prepare white-rot fungi first order seed；
Step 2, deodorant fluid microbial inoculum amount mixed fungus fermentation：
The saccharomyces cerevisiae first order seed that is obtained for step 1, lactobacillus acidophilus first order seed, bafillus natto first order seed,
Bacillus subtilis first order seed and white-rot fungi first order seed, by the parts by weight of saccharomyces cerevisiae first order seed 30~70, acidophilus breast
The parts by weight of bacillus first order seed 10~60, the parts by weight of bafillus natto first order seed 5~30, bacillus subtilis first order seed
The ratio inoculum concentration of 5~20 parts by weight and the parts by weight of white-rot fungi first order seed 10~30 is inoculated into liquid fermentation tank culture medium
Fermentation；Wherein, fermentation is：28~45 DEG C of temperature, throughput control be 0.5~2L/min, speed of agitator 300~
500rpm, coefficient control is 75%, and pressure 0.1MPa ferments 3~5 days, prepares liquid deodorant microbial inoculum.
6. house refuse according to claim 5 and the preparation method for cultivating fecal deodorizing microbial bacterial agent, its feature
It is, in step 2, liquid fermentation tank culture medium composition is：The parts by weight of corn flour 2~5, the parts by weight of glucose 1~2, corn steep liquor 5
~10 parts by weight, remaining is water.
7. house refuse according to claim 5 and the preparation method for cultivating fecal deodorizing microbial bacterial agent, its feature
It is, before step 1, in addition to：
The preparation of initial strain：Respectively from household refuse landfill sites, plant's animal wastes collecting sampling, by sample sterilized water
After 50 times of dilution, in shaking 1~2h under conditions of 200 revs/min on concussion bed, the then inoculation using mass fraction as 5%
Amount is respectively connected to cultivate 5 days in LB culture mediums and PDA culture medium, and so repeatedly 5 times, pregnant solution then is diluted into 108~1010
Times, on the flat board for being coated on LB and PDA selectivity, select the typical single bacterium colony of form and carry out test tube slant preservation, and censorship
16S RNA are identified, five plants of deodorization strains are screened altogether, are respectively：Saccharomyces cerevisiae, lactobacillus acidophilus, bacillus subtilis, natto
Bacillus and white-rot fungi；
Activation：The strain of 4 DEG C of preservations is subjected to slant activation, i.e.,：Respectively by saccharomyces cerevisiae, lactobacillus acidophilus, natto gemma bar
Bacterium, bacillus subtilis are inoculated in LB inclined-planes, are activated 2 days under conditions of 35 DEG C, saccharomyces cerevisiae, acidophilus breast after being activated
Bacillus, bafillus natto, bacillus subtilis；White-rot fungi is inoculated in PDA inclined-planes, activated 3 days under conditions of 37 DEG C,
White-rot fungi after being activated.
8. a kind of house refuse and the purposes for cultivating fecal deodorizing microbial bacterial agent, it is characterised in that microbial bacterial agent is used for
House refuse and cultivation fecal deodorizing, its deodorisation process is：Microbial bacterial agent is put into house refuse and excrement, microorganism
Saccharomyces cerevisiae and lactobacillus acidophilus in microbial inoculum suppress corrupt class bacterial reproduction in house refuse and excrement, while life can be removed
Rubbish and ammonia and hydrogen sulfide gas in excrement living；Bafillus natto and producing bacillus subtilis life neutral proteinase, egg
White enzyme can decompose the organic nitrogen of the protein-based generation stink of excrement class, and be translated into nitrate nitrogen, reach deodorization；In vain
The material that rotten Fungi Degrading Lignin hardly possible is decomposed, while organic sulfur odorous gas of degrading.
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Application publication date: 20170922 |