CN104152386B - A kind of multi-functional probiotics - Google Patents
A kind of multi-functional probiotics Download PDFInfo
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- CN104152386B CN104152386B CN201410398072.6A CN201410398072A CN104152386B CN 104152386 B CN104152386 B CN 104152386B CN 201410398072 A CN201410398072 A CN 201410398072A CN 104152386 B CN104152386 B CN 104152386B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a kind of multi-functional probiotics for belonging to microorganism formulation preparing technical field.It includes the active material of following component：Lactobacillus casei, bacillus licheniformis, bacillus subtilis, Bacillus cercus, saccharomyces cerevisiae and Pseudomonas stutzeri.The multi-functional Tiny ecosystem composition of the present invention can prepare beneficial bacterium feed with wiring solution-forming, available for the deodorization of animal wastes, prevent and treat letting animals feed enteritis and diarrhoea, have a wide range of application.
Description
Technical field
The invention belongs to microorganism formulation preparing technical field, and in particular to a kind of multi-functional probiotics, have and remove
It is smelly, letting animals feed enteritis is treated, big date fruit is cultivated and adds lustre to, the multiple efficacies such as increase.
Background technology
At present, home poultry raising has become a highly developed technology, but the management of poultry manure is also present a lot
The place of deficiency, subject matter are that raising place excrement is difficult to timely processing, and raising place stink is heavier, is unfavorable for the life of animal
Long health is, it is necessary to take a large amount of antibiotic to maintain the life of raise poultry, and the poultry quality produced is low, containing a variety of antibiosis
Element.
Enteritis and diarrhoea are the most common diseases of letting animals feed.Its cause of disease is complicated, and morbidity is anxious, and the death rate is high, dynamic to raising
Thing produces to form larger threat.Diarrhoea and enteritis are as follows according to its Symptomatic classification：Three kinds, i.e. intestines are divided into according to the situation of excrement
Scorching, mucoenteritis and diarrhoea；Every generation is suffered from diarrhoea and the intestinal mucosa person of coming off is classified as enteritis, and is divided into according to occurring degree
Serious enteritis and slight enteritis two types, morbidity is heavier, and dehydration, appetite reduce or the trencherman that gives up is classified as serious enteritis；Morbidity is just
Phase, slight enteritis is classified as without substantially dehydration and the fine person of appetite；Mucoenteritis is excrement deformation, with gel-shaped mucus
Person；Soft stool and loose stools occur for diarrhoea, and no gel-shaped mucus and intestinal mucosa do not come off person.Common antibiotics or change in production
Medical treatment is learned, in spite of certain effect, but its drawback is also obvious.It is green in particular with the rise of green waves
Color consumption as society main flow, in husbandry sector the use of antibiotic and chemicals be very restricted.Therefore, select
Select its substitute long-term as one of animal and veterinary scientific and technical personnel and difficult task.
Jujube be it is a kind of common cultivate apple trees, the larger jujube of sugariness is particularly subject to liking for people, and still, jujube exists
After maturation, the epidermis contracting wrinkle of jujube, or even segmentation crack is produced, and the aesthetics of jujube is influenceed, the pulp of the inside also tails off after shrink,
Influence mouthfeel, jujube is in growth course, because its sugariness is big, is subject to insect pest, conventional method using pesticide spraying not into
The method of ripe fruit, cause the residual of agricultural chemicals.
The content of the invention
It is an object of the invention to provide a kind of multi-functional Tiny ecosystem composition, has deodorization, treats letting animals feed enteritis,
Cultivate big date fruit to add lustre to, the multiple efficacies such as increase.
A kind of multi-functional Tiny ecosystem composition, include the active material of following component：Lactobacillus casei, lichens gemma bar
Bacterium, bacillus subtilis, Bacillus cercus, saccharomyces cerevisiae and Pseudomonas stutzeri.
The active component of the Tiny ecosystem composition also includes Lactobacillus harbinensis.
The nutrient solution of above-mentioned multi-functional Tiny ecosystem composition, the nutrient solution are made up of following components：Deionized water
1000mL, yeast extract 4-6g, beef freeze 1-2g, sucrose 5-10g, Lactobacillus casei 5-15x108Cfu/mL, bacillus licheniformis
5-15x108Cfu/mL, bacillus subtilis 5-15x108Cfu/mL, Bacillus cercus 5-15x108Cfu/mL, saccharomyces cerevisiae
5-15x108Cfu/mL and Pseudomonas stutzeri 5-15x108cfu/mL。
The nutrient solution of above-mentioned multi-functional Tiny ecosystem composition, the nutrient solution are made up of following components：Deionized water
1000mL, yeast extract 4-6g, beef freeze 1-2g, sucrose 5-10g, Lactobacillus casei 5-15x108Cfu/mL, bacillus licheniformis
5-15x108Cfu/mL, bacillus subtilis 5-15x108Cfu/mL, Bacillus cercus 5-15x108Cfu/mL, saccharomyces cerevisiae
5-15x108Cfu/mL, Pseudomonas stutzeri 5-15x108Cfu/mL and Lactobacillus harbinensis 5-15x
108cfu/mL。
A kind of deodorization probiotics, the probiotics by following weight material composition：The institute of claim 3 or 4
The nutrient solution 1-3mL of multi-functional Tiny ecosystem composition, brown sugar 1-2g, lactose 1-2g, calcium carbonate 1-2g, peptone 1-2g are stated,
MgSO40.1-0.2g, water 92-98mL.
A kind of preparation method for the feed for preventing letting animals feed enteritis and diarrhoea, is carried out in accordance with the following steps：
(1) maize straw, beanstalk and peanut seedling are crushed；Mixed roughage is mixed into according to mass ratio 1: 1: 1；
(2) into mixed roughage, sprinkling accounts for the brown sugar that its mass percent is 0.5-1%, or addition accounts for its quality hundred
Divide the corn flour than 4-8%, addition roughage is made；
(3) water for accounting for its mass percent 50-55% is added into addition roughage described in step (2), is stirred,
It is made and adds water feed；
(4) to the culture for adding in water feed sprinkling to account for the multi-functional Tiny ecosystem composition that its mass ratio is 0.1-1%
Liquid；
(5) anaerobic fermentation 7-30 days, dry standby.
For above-mentioned multi-functional Tiny ecosystem composition in deodorization, jujube goes wrinkle to add lustre to and prevent and treat letting animals feed enteritis and abdomen
Application in rushing down.
Beneficial effects of the present invention：The multi-functional Tiny ecosystem composition of the present invention can prepare beneficial bacterium feeding with wiring solution-forming
Material, available for the deodorization of animal wastes, letting animals feed enteritis and diarrhoea are prevented and treated, is had a wide range of application.
Embodiment
With reference to specific embodiment, the present invention will be further described.
Embodiment 1
A kind of nutrient solution of multi-functional Tiny ecosystem composition, the nutrient solution are made up of following components：Deionized water
1000mL, yeast extract 5g, beef freeze 1.5g, sucrose 8g, Lactobacillus casei 10x108Cfu/mL, bacillus licheniformis 8x108cfu/
ML, bacillus subtilis 11x108Cfu/mL, Bacillus cercus 10x108Cfu/mL, saccharomyces cerevisiae 8x108Cfu/mL and Amur
Pseudomonad 10x108cfu/mL。
Nutrient solution 1mL, brown sugar 1g, lactose 1g, calcium carbonate 1g, the peptone 1g of above-mentioned multi-functional Tiny ecosystem composition are taken,
MgSO40.1g, add water 92mL, be well mixed, deodorization solution is made.
Above-mentioned deodorization solution 100mL is taken, sprays once be sprayed on 100m week about2Hen house ground, the excrement in hen house
Just stink substantially eliminates.
Embodiment 2
A kind of nutrient solution of multi-functional Tiny ecosystem composition, the nutrient solution are made up of following components：Deionized water
1000mL, yeast extract 6g, beef freeze 2g, sucrose 10g, Lactobacillus casei 15x108Cfu/mL, bacillus licheniformis 5x108cfu/
ML, bacillus subtilis 5x108Cfu/mL, Bacillus cercus 15x108Cfu/mL, saccharomyces cerevisiae 5x108Cfu/mL and Amur
Pseudomonad 15x108cfu/mL。
Nutrient solution 3mL, brown sugar 2g, lactose 2g, calcium carbonate 2g, the peptone 2g of above-mentioned multi-functional Tiny ecosystem composition are taken,
MgSO40.2g, add water 98mL, be well mixed, deodorization solution is made.
Above-mentioned deodorization solution 100mL is taken, sprays once be sprayed on 100m week about2Hen house ground, the excrement in hen house
Just stink substantially eliminates.
Embodiment 3
A kind of nutrient solution of multi-functional Tiny ecosystem composition, the nutrient solution are made up of following components：Deionized water
1000mL, yeast extract 5g, beef freeze 2g, sucrose 8g, Lactobacillus casei 10x108Cfu/mL, bacillus licheniformis 10x108cfu/
ML, bacillus subtilis 10x108Cfu/mL, Bacillus cercus 10x108Cfu/mL, saccharomyces cerevisiae 10x108Cfu/mL, Amur
Pseudomonad 10x108Cfu/mL and Lactobacillus harbinensis 10x 108cfu/mL。
Take the nutrient solution 2mL of above-mentioned multi-functional Tiny ecosystem composition, brown sugar 1.5g, lactose 1.5g, calcium carbonate 1.5g, albumen
Peptone 1.5g, MgSO40.15g, add water 96mL, be well mixed, deodorization solution is made.
Above-mentioned nutrient solution 100mL is taken, sprays once be sprayed on 100m week about2Hen house ground, the excrement in hen house
Stink substantially eliminates.
Embodiment 4
A kind of nutrient solution of multi-functional Tiny ecosystem composition, the nutrient solution are made up of following components：Deionized water
1000mL, yeast extract 6g, beef freeze 1g, sucrose 5g, Lactobacillus casei 5x108Cfu/mL, bacillus licheniformis 15x108cfu/
ML, bacillus subtilis 15x108Cfu/mL, Bacillus cercus 15x108Cfu/mL, saccharomyces cerevisiae 15x108Cfu/mL and apply
Family name pseudomonad 15x108cfu/mL。
The method that the feed of prevention letting animals feed enteritis and diarrhoea is prepared using above-mentioned nutrient solution, is entered in accordance with the following steps
OK：
(1) maize straw, beanstalk and peanut seedling are crushed；Mixed roughage is mixed into according to mass ratio 1: 1: 1；
(2) into mixed roughage, sprinkling accounts for the brown sugar that its mass percent is 1%, or addition accounts for its mass percent
5% corn flour, addition roughage is made；
(3) water for accounting for its mass percent 52% is added into addition roughage described in step (2), is stirred, is made
Cheng Jiashui feeds；
(4) to the nutrient solution for adding in water feed sprinkling to account for the multi-functional Tiny ecosystem composition that its mass ratio is 0.5%；
(5) anaerobic fermentation 7-30 days, dry standby.
After drying, basis is substituted respectively according to the 3% of daily ration, 5%, 7% biologic coarse fodder (experiment I, II, III group)
Equivalent wheat bran in daily ration, control group fed basal diet.
As a result show (table 1)：The roughage of fermentation can substitute wheat bran and feed rabbit, improve feed intake, the speed of growth
21.74%, reduce food consumption 16.33%.
Influence of the fermentation roughage of table 1 to Rex Rabbit production performance
| Group | Starting weight (g/ is only) | End weight (g/ is only) | Daily gain (g/ is only) | Daily material consumption (g/ is only) | Feed consumption weightening ratio |
| Try I | 614.60±27.12 | 1483.16±31. | 31.02±0.51Aa | 93.54±0.14Aa | 3.02±0.51a |
| Try II | 610.00±29.58 | 1423.96±37. | 29.07±0.76Ab | 91.25±1.81ABab | 3.14±0.42a |
| Try III | 613.15±15.27 | 1471.91±14. | 30.67±0.40Aa | 92.15±1.03ABa | 3.00±0.21a |
| Control group | 610.94±30.26 | 1324.38±41. | 25.48±0.08Bc | 88.99±1.51Bb | 3.49±1.02a |
Feeding fermentation High-Forage Diets can significantly reduce diarrhea rate (table 2), and in the range of addition 3~7%, with
The increase of addition, effect is more notable.
Influence of the fermentation roughage of the varying level of table 2 to Rex Rabbit diarrhea rate
| Group | Try I | Try II | Try III | Control group |
| Diarrhea frequency (%) | 0.54 | 0.36 | 0.19 | 2.14 |
Addition fermentation roughage can significantly improve feed digestibility in daily ration：Thick protein 5~7, crude fibre 4~5, thick fat
2~3 percentage points of fat.
Influence of the fermentation roughage of table 3 to Rex Rabbit nutritive digestibility
| Group | Crude protein (%) | Crude fibre (%) | Crude fat (%) |
| Try I | 75.87±0.73A | 23.95±1.87a | 76.93±0.84ab |
| Try II | 75.32±1.09A | 24.64±1.09a | 77.72±1.36ab |
| Try III | 77.04±0.64A | 25.01±1.21a | 78.80±1.06a |
| Control group | 70.76±1.21B | 20.07±2.43b | 75.01±2.12b |
Embodiment 5
A kind of nutrient solution of multi-functional Tiny ecosystem composition, the nutrient solution are made up of following components：Deionized water
1000mL, yeast extract 6g, beef freeze 2g, sucrose 10g, Lactobacillus casei 15x108Cfu/mL, bacillus licheniformis 15x108cfu/
ML, bacillus subtilis 15x108Cfu/mL, Bacillus cercus 15x108Cfu/mL, saccharomyces cerevisiae 15x108Cfu/mL, Amur
Pseudomonad 15x108Cfu/mL and Lactobacillus harbinensis 5-15x108cfu/mL。
The method that the feed of prevention letting animals feed enteritis and diarrhoea is prepared using above-mentioned nutrient solution, is entered in accordance with the following steps
OK：
(1) maize straw, beanstalk and peanut seedling are crushed；Mixed roughage is mixed into according to mass ratio 1: 1: 1；
(2) into mixed roughage, sprinkling accounts for the brown sugar that its mass percent is 0.6%, or addition accounts for its quality percentage
Corn flour than 8%, addition roughage is made；
(3) water for accounting for its mass percent 53% is added into addition roughage described in step (2), is stirred, is made
Cheng Jiashui feeds；
(4) to the nutrient solution for adding in water feed sprinkling to account for the multi-functional Tiny ecosystem composition that its mass ratio is 0.6%；
(5) anaerobic fermentation 7-30 days, dry standby.
After drying, basis is substituted respectively according to the 3% of daily ration, 5%, 7% biologic coarse fodder (experiment I, II, III group)
Equivalent wheat bran in daily ration, control group fed basal diet.
As a result show (table 4)：The roughage of fermentation can substitute wheat bran and feed rabbit, improve feed intake, the speed of growth
32.74%, reduce food consumption 27.33%.
Influence of the fermentation roughage of table 4 to Rex Rabbit production performance
| Group | Starting weight (g/ is only) | End weight (g/ is only) | Daily gain (g/ is only) | Daily material consumption (g/ is only) | Feed consumption weightening ratio |
| Try I | 614.60±27.12 | 1783.16±31. | 41.02±0.51Aa | 83.54±0.14Aa | 3.02±0.51a |
| Try II | 610.00±29.58 | 1723.96±37. | 40.07±0.76Ab | 81.25±1.81ABab | 3.14±0.42a |
| Try III | 613.15±15.27 | 1771.91±14. | 39.67±0.40Aa | 82.15±1.03ABa | 3.00±0.21a |
| Control group | 610.94±30.26 | 1324.38±41. | 25.48±0.08Bc | 88.99±1.51Bb | 3.49±1.02a |
Feeding fermentation High-Forage Diets can significantly reduce diarrhea rate (table 5), and in the range of addition 3~7%, with
The increase of addition, effect is more notable.
Influence of the fermentation roughage of the varying level of table 5 to Rex Rabbit diarrhea rate
| Group | Try I | Try II | Try III | Control group |
| Diarrhea frequency (%) | 0.44 | 0.33 | 0.12 | 2.14 |
Addition fermentation roughage can significantly improve feed digestibility in daily ration：Thick protein 5~7, crude fibre 4~5, thick fat
2~3 percentage points of fat.
Influence of the fermentation roughage of table 6 to Rex Rabbit nutritive digestibility
| Group | Crude protein (%) | Crude fibre (%) | Crude fat (%) |
| Try I | 75.88±0.73A | 22.95±1.87a | 76.93±0.84ab |
| Try II | 74.32±1.09A | 22.64±1.09a | 77.72±1.36ab |
| Try III | 76.04±0.64A | 24.01±1.21a | 78.80±1.06a |
| Control group | 71.76±1.21B | 21.07±2.43b | 75.01±2.12b |
Claims (3)
1. a kind of nutrient solution of multi-functional Tiny ecosystem composition, it is characterised in that the nutrient solution is made up of following components：Go from
Sub- water 1000mL, yeast extract 4-6g, beef peptone 1-2g, sucrose 5-10g, Lactobacillus casei 5-15x108Cfu/mL, lichens gemma
Bacillus 5-15x108Cfu/mL, bacillus subtilis 5-15x108Cfu/mL, Bacillus cercus 5-15x108Cfu/mL, wine brewing
Yeast 5-15x108Cfu/mL, Pseudomonas stutzeri 5-15x108Cfu/mL and Lactobacillus harbinensis 5-
15x108cfu/mL。
A kind of 2. deodorization probiotics, it is characterised in that the probiotics by following weight material composition：Right will
Seek the nutrient solution 1-3mL, brown sugar 1-2g, lactose 1-2g, calcium carbonate 1-2g, peptone 1- of the 1 multi-functional Tiny ecosystem composition
2g, MgSO40.1-0.2g, water 92-98mL.
3. a kind of preparation method for the feed for preventing letting animals feed enteritis and diarrhoea, it is characterised in that carry out in accordance with the following steps：
(1) maize straw, beanstalk and peanut seedling are crushed；Mixed roughage is mixed into according to mass ratio 1: 1: 1；
(2) into mixed roughage, sprinkling accounts for the brown sugar that its mass percent is 0.5-1%, or addition accounts for its mass percent
4-8% corn flour, addition roughage is made；
(3) water for accounting for its mass percent 50-55% is added into addition roughage described in step (2), stirs, is made
Add water feed；
(4) to adding in water feed sprinkling to account for multi-functional Tiny ecosystem composition described in the claim 1 that its mass ratio is 0.1-1%
Nutrient solution；
(5) anaerobic fermentation 7-30 days, dry standby.
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104322894A (en) * | 2014-11-28 | 2015-02-04 | 蒋小春 | Preparation method of microbial preparation for feed |
| CN104397357A (en) * | 2014-12-11 | 2015-03-11 | 河北农业大学 | Premix for improving breeding performance of female rabbits |
| CN104686838A (en) * | 2015-02-11 | 2015-06-10 | 青岛根源生物技术集团有限公司 | Composite microecological feed additive capable of increasing freshwater fish growth rate and application of composite microecological feed additive |
| CN104814355A (en) * | 2015-05-23 | 2015-08-05 | 昆明饲宝饲料有限责任公司 | Deodorant traditional Chinese medicine feed additive for chicken, preparation method and application |
| CN106720942A (en) * | 2016-12-15 | 2017-05-31 | 上海创博生态工程有限公司 | A kind of additive for microbe feedstuff for improving Eriocheir sinensis meat and preparation method thereof |
| CN108823114A (en) * | 2018-05-02 | 2018-11-16 | 中国石油大学(华东) | A kind of cultural method of rubbish deodorization biological enzyme and its application |
| CN109430521A (en) * | 2018-12-29 | 2019-03-08 | 上海邦成生物工程有限公司 | A kind of compound feeding micro-ecological preparation and preparation method thereof |
| CN110699299B (en) * | 2019-11-14 | 2023-01-17 | 乌拉特前旗荣生大地生物科技饲料有限责任公司 | Bacillus licheniformis X173 strain for producing urease inhibitor and application thereof |
| CN113278560A (en) * | 2021-06-15 | 2021-08-20 | 无锡拜弗德生物科技有限公司 | Microecological preparation for improving pet odor |
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| CN1682599A (en) * | 2004-04-14 | 2005-10-19 | 中国农业大学 | Microbial fodder additive and its preparing method and use |
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