CN104450573A - Compound ecological microbial agent containing microbial polysaccharide and functional microbes - Google Patents
Compound ecological microbial agent containing microbial polysaccharide and functional microbes Download PDFInfo
- Publication number
- CN104450573A CN104450573A CN201410742929.1A CN201410742929A CN104450573A CN 104450573 A CN104450573 A CN 104450573A CN 201410742929 A CN201410742929 A CN 201410742929A CN 104450573 A CN104450573 A CN 104450573A
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- Prior art keywords
- microbial agent
- bacillus
- polysaccharide
- microbial
- composite ecological
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
Abstract
The invention provides a compound ecological microbial agent containing microbial polysaccharide and functional microbes. The microbial agent is a compound product of bacillus mucilaginosus, bacillus subtilis, bacillus megatherium, bacillus thuringiensis and other strains, and is mainly applied to crop seed dressing and bio-fertilizer. The compound ecological microbial agent is applied to crop planting, and can be used for effectively improving the soil fertility and soil property and increasing the yield of crops.
Description
Technical field
The invention belongs to biological technical field, the present invention relates to biotechnology and probiotics field.
Background technology
Colloid bacillus cereus, is the large pod membrane bacillus of a class, can produces a large amount of microbial polysaccharide, form zoogloea, there is water conservation moisturizing, ability that phosphorus fixed nitrogen is released in potassium decomposing, its secretion plant growth substance and multiple enzyme, can strengthen the resistibility of crop to some diseases simultaneously.Subtilis has the effect that diseases prevention is antibacterial, strengthen plant disease-resistant performance, bacillus megaterium has the effect of the solid potassium of phosphorus decomposing, and Bacillus thuringiensis has desinsection, the effect of disease prevention, above four kinds of bacterial strains are root system of plant growth-promoting bacterium, four synergies, get advantage mutually, effectively can promote the conversion of soil inavailable phosphorus potassium, increase the supply of soil phosphorus potassium, strengthen crop drought resistance disease prevention ability, and then improve the output of crop.
Summary of the invention
The object of the present invention is to provide one effectively to increase soil fertility, strengthen crop drought resistance disease prevention ability, improve composite ecological microbial agent of crop yield and preparation method thereof.
The preparation method of invention formulation:
1, colloid bacillus cereus
1.1 bacterium sources: research department screens, be preserved in China Committee for Culture Collection of Microorganisms of the Institute of Microorganism, Academia Sinica common micro-organisms center being positioned at No. 3, No. 1, Chaoyang District Beijing North Star West Road institute, preserving number: CGMCC No.5766, preservation date: on February 16th, 2012, Classification And Nomenclature is: colloid bacillus cereus (Bacillus mucilaginosus).
1.2 fermentation process:
Fermention medium (g/L): sucrose 62, urea 0.3, CaCO
35, K
2hPO
43H
2o 0.17, MgSO
47H
2o 0.6, NaCl 0.4, pH 7.0.
Culture condition: 50mL fermention medium/500mL triangular flask, 200rpm shaker fermentation, leavening temperature 30-32 DEG C, fermentation time 60-65h.
2, the preparation of composite ecological microbial agent
According to above-mentioned fermentation process, obtained colloid bacillus cereus bacterium liquid, its colloid bacillus cereus viable bacteria content is 10
8-10
9cfu/mL, microbial polysaccharide content is 2-3%.Commercially available trehalose 15-20g/L is added in fermented liquid, add subtilis that commercially available viable bacteria content is 10,000,000,000 cfu/g, bacillus megaterium, bacillus thuringiensis bacterium powder, make colloid bacillus cereus in bacterium liquid: subtilis: bacillus megaterium: the viable bacteria ratio of bacillus thuringiensis is about 2: 1: 1: 1, makes composite ecological microbial agent.
The composite bacterium powder being rich in polysaccharide made by above-mentioned microbial inoculum through atomizing freeze drying, be convenient to preservation and transport.
[beneficial effect]
Provide a kind of composite ecological microbial agent containing microbial polysaccharide and functional microorganism and preparation method thereof.This composite ecological microbial agent action effect is: (1), as biological fertilizer additive, with the commercially available peat composed of rotten mosses, composite fertilizer's conbined usage, to increase soil fertility, moisture holding capacity fast and effectively, reduces crop pest, promotes increasing crop yield; (2) long-term continuing uses, and can gradually reduce the usage quantity of chemical fertilizer, effectively improve soil regime; (3) as seed dressing, the output of the farm crop such as rape, soybean can effectively be improved.
Embodiment
Embodiment 1: the fermentation culture of colloid bacillus cereus
With the bacterial classification that deposit number is CGMCC No.5766, turn from the inclined-plane preserved and press on fresh inclined-plane, in 30-32 DEG C of constant temperature culture 48h.From inclined plane inoculating one ring to 50ml seed culture medium, 30-32 DEG C, 200rpm shaking table concussion cultivation 24h.Load 50mL fermention medium in 500mL triangular flask, with the inoculum size access seed liquor of 4%, cultivate the fermented liquid that 60-65h obtains colloid bacillus for 200rpm, 30-32 DEG C, cell concentration is about 5g/L, and polysaccharide concentration is about 25g/L.
Embodiment 2: the preparation method of composite ecological microbial agent
Commercially available trehalose 15-20g/L is added in colloid bacillus cereus bacterium liquid described in embodiment 1, add subtilis that commercially available viable bacteria content is 10,000,000,000 cfu/g, bacillus megaterium, bacillus thuringiensis bacterium powder, make colloid bacillus cereus in bacterium liquid: subtilis: bacillus megaterium: the viable bacteria ratio of bacillus thuringiensis is about 2: 1: 1: 1, make composite ecological microbial agent, its colloid bacillus cereus viable bacteria content is 10
8-10
9cfu/mL, microbial polysaccharide content is 2-3%, and small molecules content of trehalose is 1-2%.
Embodiment 3: the effect of composite ecological microbial agent
Soybean in field is tested
For more commercially available colloid bacillus cereus and difference of being rich in the colloid bacillus cereus of polysaccharide of the present invention, carry out soybean in field test.Control group: replace colloid bacillus cereus bacterium liquid described in embodiment 1 with commercially available colloid bacillus cereus bacterium powder, prepare composite ecological microbial agent by the method for embodiment 2.Experimental group: adopt the colloid bacillus cereus bacterium liquid described in embodiment 1, the composite ecological microbial agent prepared by the method for embodiment 2, is rich in microbial polysaccharide.Result is as shown in table 1: experimental group soybean yields and quality all comparatively control group are high, and according to soil data display, experimental group soil fertility improves, and water-stable granule increases, and illustrates that the function of soil water storage fertilizer conservation is improved.
Experiment kind: soybean; Experiment area: 1 mu/group; Rate of fertilizer: 80kg/ mu.
Table 1
Soil fertility: effectively potassium+available phosphorus+available nitrogen (g/kg)
Embodiment 3: the effect of composite ecological microbial agent
Rape field test
Control group: replace colloid bacillus cereus bacterium liquid described in embodiment 1 with commercially available colloid bacillus cereus bacterium powder, prepare composite ecological microbial agent by the method for embodiment 2.Experimental group: adopt the colloid bacillus cereus bacterium liquid described in embodiment 1, the composite ecological microbial agent prepared by the method for embodiment 2, is rich in microbial polysaccharide.Carry out the rape field test of 3 years by a definite date, investigate two kinds of composite ecological microbial agents to yield of rape and the impact of quality and the impact of soil conditions, result is as Table 2,3: experimental group rape growing way and output all comparatively control group are good, and along with the increase of the experiment time limit, experimental group advantage is more and more obvious, according to soil data display, experimental group soil fertility improves, and water-stable granule increases.
Experiment kind: rape; Experiment area: 1 mu/group; The total amount of application of fertilizer: 80kg/ mu.
Table 2 yield of rape changes
Soil conditions change after 3 years tested by table 3
| Soil fertility (g/kg) | Water-stable granule (0.2-2mm) % | |
| Control group | 2.934 | 17.5 |
| Experimental group | 4.416 | 41.9 |
Soil fertility: effectively potassium+available phosphorus+available nitrogen (g/kg)
According to above-mentioned field test results, the composite ecological microbial agent of polysaccharide is rich in the present invention, effectively can improve the ratio of water-stable granule in soil, improves the ability of soil water storage fertilizer conservation; Effectively improve the content of effectively N P and K in soil simultaneously, increase soil fertility, promote the growth of root system of plant, improve crop yield and quality.
Claims (4)
1. the composite ecological microbial agent containing microbial polysaccharide and functional microorganism, is characterized in that: this microbial inoculum is containing colloid bacillus cereus and subtilis, bacillus megaterium, bacillus thuringiensis.
2. composite ecological microbial agent as claimed in claim 1, is characterized in that: wherein colloid bacillus cereus viable bacteria content is 10
8-10
9cfu/mL, the microbial polysaccharide containing 2-3% in bacterium liquid, the small molecules trehalose of 1-2%.
3. the preparation method of composite ecological microbial agent described in claim 1, it is characterized by: colloid bacillus cereus deep layer liquid state fermentation 60-65h obtains sporeformer liquid, commercially available trehalose 15-20g/L is added in bacterium liquid, add subtilis that commercially available viable bacteria content is 10,000,000,000 cfu/g, bacillus megaterium, bacillus thuringiensis bacterium powder simultaneously, make colloid bacillus cereus in bacterium liquid: subtilis: bacillus megaterium: the viable bacteria ratio of bacillus thuringiensis is about 2: 1: 1: 1, makes composite ecological microbial agent.
4. the preparation method of composite ecological microbial agent as claimed in claim 3, is characterized by: can make through atomizing freeze drying the bacterium powder being rich in polysaccharide, be convenient to preservation and transport.
Priority Applications (1)
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| CN201410742929.1A CN104450573A (en) | 2014-12-02 | 2014-12-02 | Compound ecological microbial agent containing microbial polysaccharide and functional microbes |
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|---|---|---|---|
| CN201410742929.1A CN104450573A (en) | 2014-12-02 | 2014-12-02 | Compound ecological microbial agent containing microbial polysaccharide and functional microbes |
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Family
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105165925A (en) * | 2015-10-22 | 2015-12-23 | 苏州科大微龙农业科技有限公司 | Compound biological insecticide capable of preventing and treating prodenia litura |
| CN106550761A (en) * | 2016-11-26 | 2017-04-05 | 山西省农业科学院作物科学研究所 | A kind of implantation methods of organic millet |
| CN107227265A (en) * | 2016-10-28 | 2017-10-03 | 武汉合缘绿色生物股份有限公司 | A kind of colloid bacillus cereus bacterial strain and microbial bacterial agent |
| CN107628894A (en) * | 2017-05-23 | 2018-01-26 | 黄山中科新佳生物科技有限公司 | Composite bacteria agent increase soil fertility and its preparation method and application |
| CN109136156A (en) * | 2018-10-10 | 2019-01-04 | 皖西学院 | Solid composite bacteria agent and preparation method thereof and the application in officinal dendrobium stem plantation |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105165925A (en) * | 2015-10-22 | 2015-12-23 | 苏州科大微龙农业科技有限公司 | Compound biological insecticide capable of preventing and treating prodenia litura |
| CN107227265A (en) * | 2016-10-28 | 2017-10-03 | 武汉合缘绿色生物股份有限公司 | A kind of colloid bacillus cereus bacterial strain and microbial bacterial agent |
| CN106550761A (en) * | 2016-11-26 | 2017-04-05 | 山西省农业科学院作物科学研究所 | A kind of implantation methods of organic millet |
| CN107628894A (en) * | 2017-05-23 | 2018-01-26 | 黄山中科新佳生物科技有限公司 | Composite bacteria agent increase soil fertility and its preparation method and application |
| CN109136156A (en) * | 2018-10-10 | 2019-01-04 | 皖西学院 | Solid composite bacteria agent and preparation method thereof and the application in officinal dendrobium stem plantation |
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Address after: Xikang Road 224000 Jiangsu province Yancheng City Dafeng Economic Development Zone No. 26 Applicant after: Jiangsu Jia Feng grain & Oil Industry Co., Ltd. Applicant after: Jiangsu Hefeng grain and oil industry Co., Ltd. Applicant after: Jiangnan University Address before: Dafeng City, Jiangsu Province, 224100 Xikang Road, Yancheng City Economic Development Zone No. 26 Applicant before: Dafeng Jia Feng Grease Co., Ltd. Applicant before: DAFENG HEFENG GRAIN AND OIL INDUSTRY CO., LTD. Applicant before: Jiangnan University |
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Application publication date: 20150325 |