CN108823136A - High-activity microorganism microbial inoculum preparation method - Google Patents
High-activity microorganism microbial inoculum preparation method Download PDFInfo
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- CN108823136A CN108823136A CN201810855355.7A CN201810855355A CN108823136A CN 108823136 A CN108823136 A CN 108823136A CN 201810855355 A CN201810855355 A CN 201810855355A CN 108823136 A CN108823136 A CN 108823136A
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- microbial inoculum
- microorganism microbial
- bean sprouts
- activity microorganism
- inoculum preparation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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Abstract
The present invention relates to a kind of high-activity microorganism microbial inoculum preparation method, the production preparation fields of bacteria agent；Spawn incubation based formulas is made of the raw material of following mass percent：Bean sprouts 16-20%；Glucose 1-3%；Calcium carbonate 1-3%；Beef extract 1-3%；Yeast extract 0.5-2%；Peptone 1-3%；Magnesium sulfate 0.001-0.03%；Manganese sulfate 0.01-0.05%；Soften water surplus.Microbial inoculum performance obtained is more excellent in the present invention, therefore can create bigger productive value in use；Toxigenic capacity is low in the present invention, and microbial inoculum activity is high.
Description
Technical field
The present invention relates to a kind of high-activity microorganism microbial inoculum preparation method, the production preparation fields of bacteria agent.
Background technique
At present with the development of the times, biotechnology is more and more mature, also brings more conveniences to people's lives
And the production and preparation of advantage, especially high-activity microorganism microbial inoculum, the life of current high-activity microorganism microbial inoculum as you know
It produces and occupys an important position and act on bacterium culture medium in preparation process；
However current high-activity microorganism microbial inoculum preparation method is also and not perfect, especially bacterium culture medium again
Formula aspect, there are also the places much needed to be improved.
The formula Shortcomings of bacterium culture medium are as follows at present：
Strain is single, and active bacteria quantity is inadequate, and nutrition is not comprehensive enough, lacks mycelia early stage generation point.
Summary of the invention
It is insufficient according to prior art, the technical problem to be solved by the present invention is to：A kind of high-activity microorganism microbial inoculum is provided
The formula of preparation method, bacterium culture medium is excellent, can cultivate place's high-activity microorganism microbial inoculum, and high survival rate is trained
This is low, and production cost is effectively reduced, and improves microbial inoculum activity.
The technical solution adopted by the present invention to solve the technical problems is：A kind of high-activity microorganism microbial inoculum production system is provided
Preparation Method, Spawn incubation based formulas are made of the raw material of following mass percent：
Bean sprouts：16-20%；
Glucose：1-3%；
Calcium carbonate：1-3%；
Beef extract：1-3%；
Yeast extract：0.5-2%；
Peptone：1-3%
Magnesium sulfate：0.001-0.03%
Manganese sulfate：0.01-0.05%
Softened water：Surplus.
Spawn incubation based formulas is made of the raw material of following mass percent：
Bean sprouts：18%；
Glucose：2%；
Calcium carbonate：1%；
Beef extract：2%；
Yeast extract：1%；
Peptone：2%
Magnesium sulfate：0.025%
Manganese sulfate：0.03%
Softened water：Surplus.
The bean sprouts is bean sprouts liquid.
The high-activity microorganism microbial inoculum preparation method, includes the following steps：
--- --- test tube triangular flask is inoculated with --- incubator culture propagation --- shaking table behaviour to medium sterilization to strain improvement
Make --- fermentation (stir, defoam, feed, adjust pH value etc.) --- detection strain --- finished product.
The beneficial effects of the invention are as follows：
1, full of nutrition, especially some micronutrient levels are very high, sufficiently meet mycelia growth needs；
2, activating soil promotes soil fertility alkali resistant solution salt improvement soil and promotes absorption of the crop to nutrient；
3, crop root growth is stimulated.
4, the formula in the present invention can effectively improve the activity of product；
5, microbial inoculum performance obtained is more excellent in the present invention, therefore can create bigger production valence in use
Value；
6, toxigenic capacity is low in the present invention, and microbial inoculum activity is high.
Specific embodiment
The embodiment of the present invention is described further below：
Embodiment 1
A kind of high-activity microorganism microbial inoculum preparation method, it is characterised in that Spawn incubation based formulas is by following quality hundred
The raw material of score forms：
Bean sprouts：16%；
Glucose：3%；
Calcium carbonate：1%；
Beef extract：3%；
Yeast extract：0.5%；
Peptone：3%
Magnesium sulfate：0.001%
Manganese sulfate：0.05%
Softened water：Surplus.
The bean sprouts is bean sprouts liquid.
The high-activity microorganism microbial inoculum preparation method, includes the following steps：
--- --- test tube triangular flask is inoculated with --- incubator culture propagation --- shaking table behaviour to medium sterilization to strain improvement
Make --- fermentation --- detection strain --- finished product.
Wherein strain is lactic acid bacteria.
This product active bacteria quantity reaches 100,000,000.
Embodiment 2
A kind of high-activity microorganism microbial inoculum preparation method, Spawn incubation based formulas by following mass percent raw material
Composition：
Bean sprouts：20%；
Glucose：1%；
Calcium carbonate：3%；
Beef extract：1%；
Yeast extract：2%；
Peptone：1%
Magnesium sulfate：0.03%
Manganese sulfate：0.01%
Softened water：Surplus.
The bean sprouts is bean sprouts liquid.
The high-activity microorganism microbial inoculum preparation method, includes the following steps：
--- --- test tube triangular flask is inoculated with --- incubator culture propagation --- shaking table behaviour to medium sterilization to strain improvement
Make --- fermentation --- detection strain --- finished product.
Wherein strain is saccharomycete.
This product active bacteria quantity reaches 1,200,000,000.
Embodiment 3
A kind of high-activity microorganism microbial inoculum preparation method, Spawn incubation based formulas by following mass percent raw material
Composition：
Bean sprouts：18%；
Glucose：2%；
Calcium carbonate：1%；
Beef extract：2%；
Yeast extract：1%；
Peptone：2%
Magnesium sulfate：0.025%
Manganese sulfate：0.03%
Softened water：Surplus.
The bean sprouts is bean sprouts liquid.
The high-activity microorganism microbial inoculum preparation method, includes the following steps：
--- --- test tube triangular flask is inoculated with --- incubator culture propagation --- shaking table behaviour to medium sterilization to strain improvement
Make --- fermentation --- detection strain --- finished product.
Wherein strain is bacillus amyloliquefaciens.
This product active bacteria quantity reaches 3.2 hundred million.
Claims (4)
1. a kind of high-activity microorganism microbial inoculum preparation method, it is characterised in that Spawn incubation based formulas is by following quality percentage
Several raw material compositions：
Bean sprouts：16-20%；
Glucose：1-3%；
Calcium carbonate：1-3%；
Beef extract：1-3%；
Yeast extract：0.5-2%；
Peptone：1-3%
Magnesium sulfate：0.001-0.03%
Manganese sulfate：0.01-0.05%
Softened water：Surplus.
2. high-activity microorganism microbial inoculum preparation method according to claim 1, it is characterised in that Spawn incubation basigamy
Side is made of the raw material of following mass percent：
Bean sprouts：18%；
Glucose：2%；
Calcium carbonate：1%；
Beef extract：2%；
Yeast extract：1%；
Peptone：2%
Magnesium sulfate：0.025%
Manganese sulfate：0.03%
Softened water：Surplus.
3. high-activity microorganism microbial inoculum preparation method according to claim 1, it is characterised in that the bean sprouts is
Bean sprouts liquid.
4. high-activity microorganism microbial inoculum preparation method according to claim 1, it is characterised in that include the following steps：
--- --- test tube triangular flask is inoculated with --- incubator culture propagation --- shaking table behaviour to medium sterilization to strain improvement
Make --- fermentation --- detection strain --- finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201810855355.7A CN108823136A (en) | 2018-07-31 | 2018-07-31 | High-activity microorganism microbial inoculum preparation method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810855355.7A CN108823136A (en) | 2018-07-31 | 2018-07-31 | High-activity microorganism microbial inoculum preparation method |
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| CN108823136A true CN108823136A (en) | 2018-11-16 |
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| CN201810855355.7A Pending CN108823136A (en) | 2018-07-31 | 2018-07-31 | High-activity microorganism microbial inoculum preparation method |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104212745A (en) * | 2014-09-10 | 2014-12-17 | 山东省健牧生物药业有限公司 | Chicken microecological preparation and preparation method thereof |
| CN104789226A (en) * | 2015-03-13 | 2015-07-22 | 迪斯科化工集团股份有限公司 | Charcoal-based microbial soil conditioner and preparation method thereof |
| CN107828696A (en) * | 2017-11-30 | 2018-03-23 | 盖萌 | A kind of composite fermentation microbe soil conditioner and preparation method thereof |
| CN107828699A (en) * | 2017-12-13 | 2018-03-23 | 潍坊市华滨生物科技有限公司 | A kind of farm composite bacteria preparation and preparation method |
-
2018
- 2018-07-31 CN CN201810855355.7A patent/CN108823136A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104212745A (en) * | 2014-09-10 | 2014-12-17 | 山东省健牧生物药业有限公司 | Chicken microecological preparation and preparation method thereof |
| CN104789226A (en) * | 2015-03-13 | 2015-07-22 | 迪斯科化工集团股份有限公司 | Charcoal-based microbial soil conditioner and preparation method thereof |
| CN107828696A (en) * | 2017-11-30 | 2018-03-23 | 盖萌 | A kind of composite fermentation microbe soil conditioner and preparation method thereof |
| CN107828699A (en) * | 2017-12-13 | 2018-03-23 | 潍坊市华滨生物科技有限公司 | A kind of farm composite bacteria preparation and preparation method |
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Application publication date: 20181116 |