CN107058414A - A kind of method for preparing L alanine - Google Patents
A kind of method for preparing L alanine Download PDFInfo
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- CN107058414A CN107058414A CN201710294839.4A CN201710294839A CN107058414A CN 107058414 A CN107058414 A CN 107058414A CN 201710294839 A CN201710294839 A CN 201710294839A CN 107058414 A CN107058414 A CN 107058414A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
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- C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
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Abstract
The present invention relates to a kind of method for preparing L alanine, it is, during being cultivated using concentration of glucose for 110 125g/L dextrose culture-medium Lactobacillus delbrueckii mutant strain Lds.0108, when residual sugar content is less than 50g/L in zymotic fluid, add and cultivated with seed liquor concentration identical fresh seeds liquid, continuation to fermentation ends into zymotic fluid.Operation is simple for this method, can improve the fermentation level of L alanine, reduces residual sugar content, shortens fermentation period, improves conversion ratio.
Description
Technical field
The invention belongs to technical field of microbial fermentation, and in particular to a kind of method for preparing ALANINE.
Background technology
ALANINE is content highest amino acid in blood of human body, and tool has been widely used.ALANINE has uniqueness
Improvement local flavor effect, addition ALANINE can significantly improve the utilization of protein in food and beverage in food and beverage
Rate, so as to improve the tart flavour of organic acid；ALANINE is a kind of good diuretics, is production vitamin B6 and L- aminopropanols
Primary raw material；Addition ALANINE can also improve the performance of PLA in addition.
At present, it is industrial to use Production by Enzymes ALANINE mostly, i.e., by rich in L-Aspartic acid-β-decarboxylase activity
Microbial cell catalysis L-Aspartic acid and obtain.The characteristics of enzymatic conversion method technique is that enzyme activity is high, and equipment investment is small, extracts
Technique is simple, but primary raw material L-Aspartic acid price fluctuation is too big, it is impossible to ensure long-term steady production.In recent years, occur in that
By primary raw material of glucose, anaerobism direct fermentation produce L- propionic acid method, it is residual with the continuous accumulation of ALANINE content
Sugared content is gradually reduced, but when residual sugar is reduced to a certain extent, the metabolic capability of strain declines, and causes fermentation period to prolong
Long, residual sugar can not be consumed further, and fermentation conversion rate is difficult to further raising, extract difficult after causing, production cost increase.Cause
This, which develops a kind of method that can effectively improve ALANINE fermentation level, has realistic meaning.
The content of the invention
It is an object of the invention to provide a kind of method for preparing ALANINE, it is：It is being 110- using concentration of glucose
During 125g/L dextrose culture-medium is cultivated Lactobacillus delbrueckii mutant strain Lds.0108, when residual in zymotic fluid
When sugared content is less than 50g/L, adds and cultivated with seed liquor concentration identical fresh seeds liquid, continuation to fermentation knot into zymotic fluid
Beam.
It is above-mentioned to refer to two with seed liquor identical fresh seeds liquid in view of the particularity and experimental error of microculture
The preparation method of person is identical, and the OD values of the two size difference within 0.5.
Strain used in the present invention is：Lds.0108, is preserved in China typical culture collection center, preserving number：CCTCC
NO：M2013361.
When fermentation proceeds to the later stage, nutritional ingredient is consumed significantly, and various secondary metabolites are gradually accumulated, anti-to participating in
The nutritional ingredient that the vigor for the various enzymes answered is generated in adverse effect, the metabolic activity reduction of strain, zymotic fluid is reduced, this
When, fresh seed liquor is added into the zymotic fluid in the fermentation later stage, because strain is in logarithmic growth in fresh seeds liquid
Phase, the metabolic activity of strain is strong, and nutritional ingredient is high, and the enzyme activity for participating in metabolic response is higher, therefore in other fermentation conditions not
In the case of change, the fermentation level of ALANINE is improved, and residual sugar reduction, conversion ratio is improved, and fermentation period shortens, so as to drop
Low manufacturing cost.
It is preferred that, when residual sugar content is 30-45g/L in zymotic fluid, added into zymotic fluid identical with seed liquor concentration
Fresh seeds liquid.When residual sugar content is in the range of this, the nutritional ingredient in zymotic fluid is gradually decreased, and the metabolism of strain is lived
Power is gradually reduced, and fresh seeds liquid is added in time, and conversion ratio can be improved to greatest extent.
It is preferred that, the volume for the fresh seeds liquid added accounts for the 1.5%-3.0% for adding after fermentation liquid cumulative volume.If adding
Fresh seeds liquid very little, then strain negligible amounts, enzyme activity not enough, falls flat；If the fresh seeds liquid added
Too much, then more fermenter volume is taken, while causing unnecessary waste.
It is preferred that, the fresh seeds liquid OD values are 4.0-8.0.Now strain is in logarithmic growth in fresh seeds liquid
Phase, the metabolic activity of strain is strong, and nutritional ingredient is high, and the enzyme activity for participating in metabolic response is higher.
It is preferred that, in every liter of dextrose culture-medium, including glucose 110-125g, Na2HPO4·12H2O 20g,
KH2PO42.0g, NaCl 0.5g, MgSO4·7H2O 0.2g, small-scale inorganic salting liquid 10ml/L, surplus is purified water；
In the small-scale inorganic salting liquid：FeCl3·6H2O 2.5g/L, CoCl2·6H2O 0.2g/L, CuCl2·2H2O
0.1g/L, ZnCl20.2g/L, Na2MoO4·2H2O 0.3g/L, H3BO30.1g/L, MnCl2·4H2O 0.5g/L, purified water
Surplus.
It is preferred that, the condition of culture is：Temperature：30℃；Ventilation：0m3/ h, pressure：0.04MPa-0.06MPa；pH：
6.5-7.0.It is typically all that pH is adjusted with liquefied ammonia in actual production, on the one hand control pH, on the other hand can be supplemented inorganic
Nitrogen source.
One or more of above-mentioned preferred scheme, can be with total culture scheme：It is being using concentration of glucose
During 110-125g/L dextrose culture-medium is cultivated Lactobacillus delbrueckii mutant strain Lds.0108, work as zymotic fluid
When middle residual sugar content is less than 50g/L, adds and cultivated with seed liquor concentration identical fresh seeds liquid, continuation to hair into zymotic fluid
Ferment terminates；It is combined and uses, any one scheme of gained is in the application scope of protection after combination.
It is further preferred that method of the present invention, comprises the following steps：
1) Lactobacillus delbrueckii mutant strain Lds.0108 seed liquors are prepared；
2) volume fraction is inoculated with into the dextrose culture-medium and carries out fermented and cultured for the 5-10% seed liquor；
3) when the concentration of glucose in zymotic fluid is less than 50g/L, added into zymotic fluid and seed liquor concentration identical
Fresh seeds liquid, continues to cultivate to fermentation ends.
It is preferred that, the preparation of the seed liquor comprises the following steps：
1) aseptically, strain is inoculated on LB slant mediums, the incubated 18h under the conditions of 30 DEG C；
2) aseptically, the thalline on inclined-plane is washed down with sterile saline, bacteria suspension is made；
3) by the LB liquid medium after bacterial suspension inoculation to sterilizing, fermentation condition is：Temperature：30℃；Air mass flow：
2L/min, tank pressure 0.04-0.06MPa；Culture to OD values are 4.0-8.0.
It is furthermore preferred that method of the present invention, comprises the following steps：
1st, Lactobacillus delbrueckii mutant strain Lds.0108 seed liquors are prepared；
1) aseptically, strain is inoculated on LB slant mediums, the incubated 18h under the conditions of 30 DEG C；
2) aseptically, the thalline on inclined-plane is washed down with sterile saline, bacteria suspension is made；
3) by the LB liquid medium after bacterial suspension inoculation to sterilizing, fermentation condition is：Temperature：30℃；Air mass flow：
2L/min, tank pressure 0.04-0.06MPa；Culture to OD values are 4.0-8.0；
2nd, Lactobacillus delbrueckii mutant strain Lds.0108 culture
The seed liquor of inoculation 6% into dextrose culture-medium, in temperature：30℃；Ventilation：0m3/ h, pressure：
0.04MPa-0.06MPa；Liquefied ammonia controls pH：6.5-7.0 under conditions of fermented；
During the composition of the fresh culture is every liter of dextrose culture-medium, including glucose 110-125g,
Na2HPO4·12H2O 20g, KH2PO42.0g, NaCl 0.5g, MgSO4·7H2O 0.2g, small-scale inorganic salting liquid 10ml, it is remaining
Measure as purified water；
In the small-scale inorganic salting liquid：FeCl3·6H2O 2.5g/L, CoCl2·6H2O 0.2g/L, CuCl2·2H2O
0.1g/L, ZnCl20.2g/L, Na2MoO4·2H2O 0.3g/L, H3BO30.1g/L, MnCl2·4H2O 0.5g/L, purified water
Surplus.
3rd, fresh seeds liquid is supplemented
When the concentration of glucose in zymotic fluid is 30-45g/L, added into zymotic fluid new with seed liquor concentration identical
Fresh seed liquor, the volume to the fresh seeds liquid added accounts for and adds the 1.5%-3.0% of after fermentation liquid cumulative volume, continues to cultivate extremely
Fermentation ends.
Method of the present invention includes following beneficial effect：
The method of the present invention adds fresh seeds liquid in the later stage of culture, due to the raising of enzyme activity, can improve to culture
The utilization rate of nutritional ingredient in base, and then the yield of ALANINE is improved, it can also shorten the cycle of fermentation.Put in tank after fermentation liquid
The concentration of ALANINE brings up to 100-110g/L by the 80-90g/L for not adding seed liquor, and residual sugar content is by original 1.3-
2.1g/L is reduced to 0.5-0.8g/L, and fermentation period shortens to 36h by original 42h, and fermentation conversion rate is by original about 80%
Bring up to about 90%.
Embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.
Embodiment 1
The present embodiment is related to a kind of method for preparing ALANINE, comprises the following steps:
1st, seed liquor is prepared
(1) aseptically, Lds.0108 is inoculated on LB slant mediums, cultivated in 30 DEG C of constant incubators
18h。
(2) aseptically, the thalline on inclined-plane is washed down with 150ml sterile salines, bacteria suspension is made.
(3) by the LB liquid medium after bacterial suspension inoculation to sterilizing, 10L seeding tanks press 7L dispensings.Temperature：30℃；
Air mass flow：2L/min, tank pressure 0.04-0.06MPa；Incubation time：5.6h, OD (600nm)：4.30, culture transferring to fermented and cultured
In base.
2nd, dextrose culture-medium is prepared
Fermentation medium is prepared by following component content：Glucose 110g/L, Na2HPO4·12H2O 20g/L, KH2PO4
2.0g/L, NaCl 0.5g/L, MgSO4·7H2O 0.2g/L, small-scale inorganic salt 10ml/L, purify water surplus；
Wherein small-scale inorganic salt includes：FeCl3·6H2O 2.5g/L, CoCl2·6H2O 0.2g/L, CuCl2·2H2O
0.1g/L, ZnCl20.2g/L, Na2MoO4·2H2O 0.3g/L, H3BO30.1g/L, MnCl2·4H2O 0.5g/L, purified water
Surplus.
121 DEG C of sterilizing 30min, are cooled to after 30 DEG C, standby.
3rd, the above-mentioned seed liquor prepared is inoculated into the dextrose culture-medium by kind of an amount 6%, 100L fermentation tanks
By 70L dispensings, in 30 DEG C of fermentation temperature；Air mass flow 0m3Cultivated under conditions of/h, tank pressure 0.04-0.06MPa；Fermentation
During use liquefied ammonia to control pH for 6.5-7.0；
4th, when residual sugar content is 33.6g/L in zymotic fluid, adding fresh seeds liquid, (fresh seeds liquid is pressed and seed liquid phase
Prepared by same method, its OD value adds the 2.0% of rear total fermentating liquid volume for 4.25), the volume for the fresh seeds liquid added is accounted for,
Continue to cultivate to fermentation ends, whole fermentation period is 22h.
After fermentation ends, it is 97g/L, residual concentration of glucose that sampling, which carries out ALANINE content in HPLC measure, zymotic fluid,
For 0.5g/L, fermentation conversion rate is 88.2%.
Embodiment 2
The present embodiment is related to a kind of method for preparing ALANINE, comprises the following steps:
1st, according to method in the same manner as in Example 1, the seed liquor that OD values are 5.24 is prepared；
2nd, dextrose culture-medium is prepared
Fermentation medium is prepared by following component content：Glucose 120g/L, Na2HPO4·12H2O 20g/L, KH2PO4
2.0g/L, NaCl 0.5g/L, MgSO4·7H2O 0.2g/L, small-scale inorganic salt 10ml/L, purify water surplus；
Wherein small-scale inorganic salt includes：FeCl3·6H2O 2.5g/L, CoCl2·6H2O 0.2g/L, CuCl2·2H2O
0.1g/L, ZnCl20.2g/L, Na2MoO4·2H2O 0.3g/L, H3BO30.1g/L, MnCl2·4H2O 0.5g/L, purified water
Surplus.
By above-mentioned culture medium in 121 DEG C of sterilizing 30min, cool to after 30 DEG C, it is standby.
3rd, the above-mentioned seed liquor prepared is inoculated into the dextrose culture-medium by kind of an amount 6%, 100L fermentation tanks
By 70L dispensings, in 30 DEG C of fermentation temperature；Air mass flow 0m3Cultivated under conditions of/h, tank pressure 0.04-0.06MPa；Fermentation
During use liquefied ammonia to control pH for 6.5-7.0；
4th, when residual sugar content is 38.7g/L in zymotic fluid, adding fresh seeds liquid, (fresh seeds liquid is pressed and seed liquid phase
Prepared by same method, its OD value adds the 2.5% of rear total fermentating liquid volume for 5.36), the volume for the fresh seeds liquid added is accounted for,
Continue to cultivate to fermentation ends, whole fermentation period is 25h.
After fermentation ends, it is 107g/L, residual concentration of glucose that sampling, which carries out ALANINE content in HPLC measure, zymotic fluid,
For 0.6g/L, fermentation conversion rate is 89.2%.
Embodiment 3
The present embodiment is related to a kind of method for preparing ALANINE, comprises the following steps：
1st, seed liquor is prepared
(1) aseptically, Lds.0108 is inoculated on LB slant mediums, cultivated in 30 DEG C of constant incubators
18h。
(2) aseptically, the thalline on inclined-plane is washed down with 150ml sterile salines, bacteria suspension is made.
(3) by the LB liquid medium after bacterial suspension inoculation to sterilizing, 100L seeding tanks press 70L dispensings.Temperature：30
℃；Air mass flow：20L/min, tank pressure 0.04-0.06MPa；Incubation time：5.2h,OD(600nm)：3.25, culture transferring is to two grades
In seed culture medium.
(4) primary seed solution is inoculated in LB liquid medium, 10T seeding tanks press 7T dispensings.Temperature：30℃；Air
Flow：120m3/ h, tank pressure 0.04-0.06MPa；Incubation time：6.5h, OD (600nm)：7.65, culture transferring to fermentation medium
In.
2nd, culture medium is prepared
Fermentation medium is prepared by following component content：Glucose 125g/L, Na2HPO4·12H2O 20g/L, KH2PO4
2.0g/L, NaCl 0.5g/L, MgSO4·7H2O 0.2g/L, small-scale inorganic salt 10ml/L, purify water surplus；
Wherein small-scale inorganic salt includes：FeCl3·6H2O 2.5g/L, CoCl2·6H2O 0.2g/L, CuCl2·2H2O
0.1g/L, ZnCl20.2g/L, Na2MoO4·2H2O 0.3g/L, H3BO30.1g/L, MnCl2·4H2O 0.5g/L, purified water
Surplus.
121 DEG C of sterilizing 30min, are cooled to after 30 DEG C, standby.
3rd, the above-mentioned seed liquor prepared is inoculated into the dextrose culture-medium by kind of an amount 6%, 100T fermentation tanks
By 70T dispensings, in 30 DEG C of fermentation temperature；Air mass flow 0m3Cultivated under conditions of/h, tank pressure 0.04-0.06MPa；Fermentation
During use liquefied ammonia to control pH for 6.5-7.0；
4th, when residual sugar content is 41.2g/L in zymotic fluid, adding fresh seeds liquid, (fresh seeds liquid is pressed and seed liquid phase
Prepared by same method, its OD value adds the 3.0% of rear total fermentating liquid volume for 7.46), the volume for the fresh seeds liquid added is accounted for,
Continue to cultivate to fermentation ends, whole fermentation period is 36h.
After fermentation ends, it is 112g/L, residual concentration of glucose that sampling, which carries out ALANINE content in HPLC measure, zymotic fluid,
For 0.8g/L, fermentation conversion rate is 89.6%.
Comparative example 1
Compared with Example 1, its difference is, not additionally add fresh seeds liquid, i.e., the operation without step 4, directly
Using initial nutrient solution to completion of fermenting, cultivation cycle is 30h.
After fermentation ends, it is 88g/L, residual concentration of glucose that sampling, which carries out ALANINE content in HPLC measure, zymotic fluid,
For 2.1g/L, fermentation conversion rate is 80%.
Comparative example 2
Compared with Example 1, its difference is, when the concentration of residual sugar in solution is 24.8g/L, adds identical fresh
Seed liquor, cultivation cycle is 25h.
After fermentation ends, it is 93.5g/L that sampling, which carries out ALANINE content in HPLC measure, zymotic fluid, and residual glucose is dense
Spend for 1.0g/L, fermentation conversion rate is 85%.
Comparative example 3
Compared with Example 1, its difference is, it is fresh to add identical when the concentration of residual sugar in solution is 53.6g/L
Seed liquor, cultivation cycle is 27h.
After fermentation ends, it is 91g/L, residual concentration of glucose that sampling, which carries out ALANINE content in HPLC measure, zymotic fluid,
For 1.4g/L, fermentation conversion rate is 82.7%.
Comparative example 4
Compared with Example 1, its difference is, adds fresh seeds liquid to fresh seeds liquid and accounts for zymotic fluid cumulative volume
1.3%, cultivation cycle is 28h.
After fermentation ends, it is 90g/L, residual concentration of glucose that sampling, which carries out ALANINE content in HPLC measure, zymotic fluid,
For 1.5g/L, fermentation conversion rate is 81.8%.
Comparative example 5
Compared with Example 1, its difference is, the OD values for adding fresh seeds liquid are 3.16, and cultivation cycle is 25h.
After fermentation ends, it is 94.5g/L that sampling, which carries out ALANINE content in HPLC measure, zymotic fluid, and residual glucose is dense
Spend for 0.9g/L, fermentation conversion rate is 85.9%.
Although above having made to retouch in detail to the present invention with general explanation, embodiment and experiment
State, but on the basis of the present invention, it can be made some modifications or improvements, this is apparent to those skilled in the art
's.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, are belonged to claimed
Scope.
Claims (9)
1. a kind of method for preparing ALANINE, it is characterised in that utilizing the glucose that concentration of glucose is 110-125g/L
During culture medium is cultivated Lactobacillus delbrueckii mutant strain Lds.0108, when residual sugar content is less than 50g/ in zymotic fluid
During L, seed liquor identical fresh seeds liquid during with inoculation is added into zymotic fluid, continues to cultivate to fermentation ends.
2. according to the method described in claim 1, it is characterised in that when residual sugar content is 30-45g/L in zymotic fluid, Xiang Fa
Seed liquor concentration identical fresh seeds liquid during with inoculation is added in zymotic fluid.
3. method according to claim 1 or 2, it is characterised in that the volume for the fresh seeds liquid added is accounted for add after send out
The 1.5%-3.0% of zymotic fluid cumulative volume.
4. the method according to claim 1 or 3, it is characterised in that the fresh seeds liquid OD values are 4.0-8.0.
5. the method according to any one of Claims 1 to 4, it is characterised in that in every liter of dextrose culture-medium, including
Glucose 110-125g, Na2HPO4·12H2O 20g, KH2PO42.0g, NaCl 0.5g, MgSO4·7H2O 0.2g, micro nothing
Machine salting liquid 10ml, surplus is purified water；
In the small-scale inorganic salting liquid：FeCl3·6H2O 2.5g/L, CoCl2·6H2O 0.2g/L, CuCl2·2H2O
0.1g/L, ZnCl20.2g/L, Na2MoO4·2H2O 0.3g/L, H3BO30.1g/L, MnCl2·4H2O 0.5g/L, purified water
Surplus.
6. according to the method described in claim 1, it is characterised in that the condition of culture is：Temperature：30℃；Ventilation：0m3/
H, pressure：0.04MPa-0.06MPa；pH：6.5-7.0.
7. the method according to any one of claim 1~6, it is characterised in that comprise the following steps：
1) Lactobacillus delbrueckii mutant strain Lds.0108 seed liquors are prepared；
2) volume fraction is inoculated with into the dextrose culture-medium and carries out fermented and cultured for the 5-10% seed liquor；
3) when the concentration of glucose in zymotic fluid is less than 50g/L, added into zymotic fluid fresh with seed liquor concentration identical
Seed liquor, continues to cultivate to fermentation ends.
8. method according to claim 7, it is characterised in that the preparation of the seed liquor comprises the following steps：
1) aseptically, strain is inoculated on LB slant mediums, the incubated 18h under the conditions of 30 DEG C；
2) aseptically, the thalline on inclined-plane is washed down with sterile saline, bacteria suspension is made；
3) by the LB liquid medium after the bacterial suspension inoculation to sterilizing, fermentation condition is：Temperature：30℃；Air mass flow：
2L/min, tank pressure 0.04-0.06MPa；Culture to OD values are 4.0-8.0.
9. the method according to claim 7 or 8, it is characterised in that comprise the following steps：
A, prepare Lactobacillus delbrueckii mutant strain Lds.0108 seed liquors；
1) aseptically, strain is inoculated on LB slant mediums, the incubated 18h under the conditions of 30 DEG C；
2) aseptically, the thalline on inclined-plane is washed down with sterile saline, bacteria suspension is made；
3) by the LB liquid medium after bacterial suspension inoculation to sterilizing, fermentation condition is：Temperature：30℃；Air mass flow：2L/
Min, tank pressure 0.04-0.06MPa；Culture to OD values are 4.0-8.0；
B, Lactobacillus delbrueckii mutant strain Lds.0108 culture
The seed liquor of inoculation 6% into dextrose culture-medium, in temperature：30℃；Ventilation：0m3/ h, pressure：0.04MPa-
0.06MPa；Liquefied ammonia controls pH：6.5-7.0 under conditions of fermented；
During the composition of the fresh culture is every liter of dextrose culture-medium, including glucose 110-125g, Na2HPO4·
12H2O 20g, KH2PO42.0g, NaCl 0.5g, MgSO4·7H2O 0.2g, small-scale inorganic salting liquid 10ml, surplus are purifying
Water；
In the small-scale inorganic salting liquid：FeCl3·6H2O 2.5g/L, CoCl2·6H2O 0.2g/L, CuCl2·2H2O
0.1g/L, ZnCl20.2g/L, Na2MoO4·2H2O 0.3g/L, H3BO30.1g/L, MnCl2·4H2O 0.5g/L, purified water
Surplus；
C, supplement fresh seeds liquid
When the concentration of glucose in zymotic fluid is 30-45g/L, added into zymotic fluid and the fresh kind of seed liquor concentration identical
Sub- liquid, the volume to the fresh seeds liquid added accounts for and adds the 1.5%-3.0% of after fermentation liquid cumulative volume, continues to cultivate to fermenting
Terminate.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109055451A (en) * | 2018-09-13 | 2018-12-21 | 安徽华恒生物科技股份有限公司 | A kind of biofermentation method of l-Alanine |
| CN110438184A (en) * | 2019-08-26 | 2019-11-12 | 山东润德生物科技有限公司 | A kind of method of N-acetylglucosamine content and conversion ratio in raising fermentation liquid |
| CN110982857A (en) * | 2019-09-23 | 2020-04-10 | 安徽丰原生物化学股份有限公司 | Fermentation production method of L-alanine |
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| CN101550437A (en) * | 2009-01-20 | 2009-10-07 | 福建省建阳武夷味精有限公司 | Method for preparing fermented liquid rich in L-tryptophan by utilizing rice |
| CN102533890A (en) * | 2012-01-13 | 2012-07-04 | 中粮生物化学（安徽）股份有限公司 | Production method of lysine |
| CN103602609A (en) * | 2013-09-05 | 2014-02-26 | 淮北新旗氨基酸有限公司 | High-yield strain for producing L-alanine by fermentation and preparation method thereof |
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| CN101550437A (en) * | 2009-01-20 | 2009-10-07 | 福建省建阳武夷味精有限公司 | Method for preparing fermented liquid rich in L-tryptophan by utilizing rice |
| CN102533890A (en) * | 2012-01-13 | 2012-07-04 | 中粮生物化学（安徽）股份有限公司 | Production method of lysine |
| CN103602609A (en) * | 2013-09-05 | 2014-02-26 | 淮北新旗氨基酸有限公司 | High-yield strain for producing L-alanine by fermentation and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109055451A (en) * | 2018-09-13 | 2018-12-21 | 安徽华恒生物科技股份有限公司 | A kind of biofermentation method of l-Alanine |
| CN110438184A (en) * | 2019-08-26 | 2019-11-12 | 山东润德生物科技有限公司 | A kind of method of N-acetylglucosamine content and conversion ratio in raising fermentation liquid |
| CN110982857A (en) * | 2019-09-23 | 2020-04-10 | 安徽丰原生物化学股份有限公司 | Fermentation production method of L-alanine |
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