CN102816817A - Fermentation method of corn soaking water for producing nisin - Google Patents
Fermentation method of corn soaking water for producing nisin Download PDFInfo
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- CN102816817A CN102816817A CN2012103278024A CN201210327802A CN102816817A CN 102816817 A CN102816817 A CN 102816817A CN 2012103278024 A CN2012103278024 A CN 2012103278024A CN 201210327802 A CN201210327802 A CN 201210327802A CN 102816817 A CN102816817 A CN 102816817A
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Abstract
The invention relates to a fermentation method of corn soaking water for producing nisin and belongs to the deep processing field of corn. The method includes producing the corn soaking water into a nutrient solution of lactococcus lactis, using sodium hydroxide with a mass concentration of 15% for regulating the pH to 6.5-7.5 and regulating the temperature to 28 DEG C to 40 DEG C, adding 10% of seed liquid, and fermenting. After the fermentation, the OD value is 1.30-1.40, the lactic acid content is 0.20-0.40 g/dL, and the tilter of the nisin is 8000-15000 IU/mL. The method has the advantages that consumed energy for further evaporation concentration of the corn soaking water is greatly saved, and problems of environmental pollution and energy consumption caused by corn steep liquor production through the corn soaking water are solved.
Description
Technical field
The invention belongs to the corn deep processing field, be specifically related to a kind of plain fermentation process of coccus for preparing.
Background technology
Nisin (Nisin) can suppress, kill G
+Bacterium and gemma thereof can be degraded by human body as organic polypeptide, and it has begun to get into food-processing industry as biological preservative.Food Office of Oversight Services of China Ministry of Health has signed and issued January 19 nineteen ninety and has used the use certification of fitness of Nisin as the food preservation agent, and March 29 nineteen ninety, Nisin was put into 1990 amendmentses of international GB2760-86, had stipulated quality standard.
The fermentation process of the nisin of existing report: " a kind of substratum, its preparation method and the method for cultivating nisin "; Number of patent application 200510071945.3; The invention discloses the substratum that is used to produce nisin, its preparation method and the method for cultivating nisin.Its preparation method is that yeast proteinoid, Sunlover 10 are dropped in the material-compound tank, adds water and stirs, and then carbon source, sodium phosphate, sal epsom is dropped into successively, stirs; Send in the culture tank after regulating pH6.2 ~ 7.2, add water, feed HP steam; Sterilization, cooling inserts the food grade milk-acid bacteria; In the jar internal pressure is that 0.1 ~ 0.5 MPa, temperature are to cultivate 24 ~ 72 h under 26 ~ 37 ℃ of conditions, and the nisin activity reaches 4000 ~ 15000 IU/mL through estimation of biological potency, can stop.This substratum is favourable to the metabolism of lactococcus lactis ssp, and the time that gets into logarithmic phase shortens, and metabolism is vigorous, and sugared nitrogen consumption is accelerated, and the paracme postpones, and the nisin activity greatly improves through estimation of biological potency, and output increases greatly.
" a kind of is the technology of raw material production nisin with the plant cake ", number of patent application 200610019565.X the present invention relates to the production technology of the plain based food additive of a kind of biological antibiotic.A kind of is the technology of raw material production nisin with the plant cake, it is characterized in that it is raw material with the plant cake, with plant cake through enzymolysis, fermentation, solid-liquid separation, concentrate, saltout, dry and be packaged to be finished product.Adopt this technology, the output of nisin increases substantially, and the fermentation unit of fermented liquid is about 3000 IU/mL in the prior art suitability for industrialized production, and can reach 4500 IU/mL with the highest fermentation unit of fermented liquid that this zymotechnique makes.
At present, it is fermentation raw material that the manufacturer of nisin mainly is to use yeast extract paste, sucrose, peptone etc., and preparing culture medium is carried out fermentative prodn; But because the employed prices of raw and semifnished materials are high; Production cost is high, if use plant cake production, because of the unit low suitability for industrialized production requirement that do not reach of tiring; Cause the production cost of nisin high, be difficult to be widely applied to field of food.In order to reduce the nisin production cost, the mode that the starting material of selecting for use will be originated is abundant, preferably turn waste into wealth, want guarantor unit's output to meet or exceed present manufacturing enterprise equivalent technology index simultaneously.
Summary of the invention
The fermentation process that the present invention provides a kind of soak water of maize to be used to produce nisin, high to solve the employed prices of raw and semifnished materials that exist in the present nisin production, the problem that production cost is high.
The soak water of maize that the present invention adopted adopts following ordinary method to obtain:
Carry out enlarged culturing to producing bacterial classification, the inoculum size with 10% inserts, simultaneously corn is soaked with 1 ~ 3% sulfurous acid solution, and 50 ℃ of soaking temperatures, soak time 30 ~ 60 h, the corn after the immersion removes starch producing etc., obtains soak water of maize.
The technical scheme that the present invention takes is: comprise the following steps:
(1), the adding mass concentration is following in soak water of maize: W-Gum glucose syrup 2 ~ 6%, cane molasses 0.1 ~ 0.3%, sal epsom 0.01 ~ 0.05%, potassium primary phosphate 0.1 ~ 1%, sodium-chlor 0.01 ~ 0.05%, vitamins B
11 ~ 5 ‰ with vitamin H 0.5 ~ 1 ‰, process the streptococcus acidi lactici nutrient solution;
(2), after the above-mentioned streptococcus acidi lactici inoculum preparation, using mass concentration is that 15% sodium hydroxide is regulated pH value to 6.5 ~ 7.5,28 ~ 40 ℃ of temperature;
(3), insert 10% seed liquor, begin to mend glucose at fermentation 4 ~ 10 h, glucose content is 1% ~ 3%; Stream adds 15% ~ 40% sodium hydroxide, and fermenting process pH is controlled at 6.4 ~ 7.3; The intermittent type aerobic fermentation, fermentation period 12 ~ 24 h, after the fermentation ends, the OD value is 1.30 ~ 1.40, and lactic acid content is 0.20-0.40 g/dL, and tiring of nisin is 8000 ~ 15000 IU/mL.
At present; It is fermentation raw material that the manufacturer of nisin mainly is to use yeast extract paste, sucrose, peptone etc.; Preparing culture medium is carried out fermentative prodn; But because the employed prices of raw and semifnished materials are high, cause the production cost of nisin high, be difficult to be widely applied to field of food.
In the W-Gum production process, the corn steep liquor that produces during soaking corn (about 2 ~ 3 ° of Be ') is rich in 4% soluble proteins, 2 ~ 3% amino acid, 1% sugar and plants nutritive ingredient such as calcium acid, can be made into dry after concentrated and be about 40% steeping water.Steeping water is mainly processed low value-added product such as protein fodder, composite fertilizer and is sold.Nearly ten million ton of annual China soak water of maize generation; Per 10 tons of soak water of maize can concentrate produces 1 ton of steeping water, and this process need consumes 2 ~ 3 tons steam, produces nearly 9 tons of organic waste waters (COD2000 ~ 3000 mg/L); Consume a large amount of steam, wasted great amount of water resources.
Advantage of the present invention is through the adjustment technique for soaking corn; Make when not influencing W-Gum production; The soak water of maize that obtains is a substrate; Suitably add nutritive ingredients such as carbon source, inorganic salt and vitamin H, control fermenting process simultaneously, obtaining tiring is the nisin fermentation liquid of 8000 ~ 15000 IU/mL.
The present invention has made full use of soak water of maize and has been rich in the organotrophy composition; Produce nisin; Not only can save the primary water of batching usefulness on the one hand; Also save the energy that the further evaporation concentration of soak water of maize is consumed greatly, solved soak water of maize production bad environment pollution that steeping water caused and the energy consumption problem utilized.Basically be that the zero cost soak water of maize is a minimum medium owing to having adopted on the other hand, with the employed raw material of original nisin production technique, raw materials cost only accounts for about 30% of former technology, and the product total cost is far below other preparation technology.Make nisin can be applied even more extensively food processing field, promote people's quality of life as biological preservative.
Embodiment
The soak water of maize that the present invention adopted adopts following ordinary method to obtain:
Carry out enlarged culturing to producing bacterial classification, the inoculum size with 10% inserts, simultaneously corn is soaked with 1 ~ 3% sulfurous acid solution, 50 ℃ of soaking temperatures, and soak time 30 ~ 60 h, the corn after the immersion removes starch producing etc., obtains soak water of maize.
Embodiment 1
(1), the adding mass concentration is following in soak water of maize: W-Gum glucose syrup 2%, cane molasses 0.1%, sal epsom 0.01%, potassium primary phosphate 0.1%, sodium-chlor 0.01%, vitamins B
11 ‰ with vitamin H 0.5 ‰, process the streptococcus acidi lactici nutrient solution;
(2), after the above-mentioned streptococcus acidi lactici inoculum preparation, using mass concentration is that 15% sodium hydroxide is regulated pH value to 6.5,28 ℃ of temperature;
(3) by the seed liquor of ordinary method access 10%, begin to mend glucose at fermentation 4 h, glucose content is 1%; Stream adds 15% sodium hydroxide, and fermenting process pH is controlled at 6.4; The intermittent type aerobic fermentation, fermentation period 12h.
Embodiment 2
(1), the adding mass concentration is following in soak water of maize: W-Gum glucose syrup 4%, cane molasses 0.2%, sal epsom 0.03%, potassium primary phosphate 0.55%, sodium-chlor 0.04%, vitamins B
13 ‰ with vitamin H 0.75 ‰, process the streptococcus acidi lactici nutrient solution;
(2), after the above-mentioned streptococcus acidi lactici inoculum preparation, using mass concentration is that 15% sodium hydroxide is regulated pH value to 7.0,34 ℃ of temperature;
(3) by the seed liquor of ordinary method access 10%, begin to mend glucose at fermentation 7 h, glucose content is 2%; Stream adds 27.5% sodium hydroxide, and fermenting process pH is controlled at 6.9; The intermittent type aerobic fermentation, fermentation period 18h.
Embodiment 3
(1), the adding mass concentration is following in soak water of maize: W-Gum glucose syrup 6%, cane molasses 0.3%, sal epsom 0.05%, potassium primary phosphate 1%, sodium-chlor 0.05%, vitamins B
15 ‰ with vitamin H 1 ‰, process the streptococcus acidi lactici nutrient solution;
(2), after the above-mentioned streptococcus acidi lactici inoculum preparation, using mass concentration is that 15% sodium hydroxide is regulated pH value to 7.5,40 ℃ of temperature;
(3) by the seed liquor of ordinary method access 10%, begin to mend glucose at fermentation 10 h, glucose content is 3%; Stream adds 40% sodium hydroxide, and fermenting process pH is controlled at 7.3; The intermittent type aerobic fermentation, fermentation period 24 h.
After the fermentation ends, the OD value is 1.30 ~ 1.40, and lactic acid content is 0.20-0.40 g/dL, and tiring of nisin is 8000 ~ 15000 IU/mL.
Claims (1)
1. a soak water of maize is used to produce the fermentation process of nisin, it is characterized in that comprising the following steps:
(1), the adding mass concentration is following in soak water of maize: W-Gum glucose syrup 2 ~ 6%, cane molasses 0.1 ~ 0.3%, sal epsom 0.01 ~ 0.05%, potassium primary phosphate 0.1 ~ 1%, sodium-chlor 0.01 ~ 0.05%, vitamins B
11 ~ 5 ‰ with vitamin H 0.5 ~ 1 ‰, process the streptococcus acidi lactici nutrient solution;
(2), after the above-mentioned streptococcus acidi lactici inoculum preparation, using mass concentration is that 15% sodium hydroxide is regulated pH value to 6.5 ~ 7.5,28 ~ 40 ℃ of temperature;
(3), insert 10% seed liquor, begin to mend glucose at fermentation 4 ~ 10 h, glucose content is 1% ~ 3%; Stream adds 15% ~ 40% sodium hydroxide, and fermenting process pH is controlled at 6.4 ~ 7.3; The intermittent type aerobic fermentation, fermentation period 12 ~ 24 h, after the fermentation ends, the OD value is 1.30 ~ 1.40, and lactic acid content is 0.20-0.40 g/dL, and tiring of nisin is 8000 ~ 15000 IU/mL.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105494978A (en) * | 2015-12-21 | 2016-04-20 | 河南省新大牧业有限公司 | Method for preparing fermented pig feed through corn soaking solution and fermented pig feed prepared through method |
| CN107400688A (en) * | 2017-06-08 | 2017-11-28 | 伟日（山东）生物科技有限公司 | Utilize the method for Wastage of food industry production nisin |
| CN112175999A (en) * | 2019-07-05 | 2021-01-05 | 中粮生物化学（安徽）股份有限公司 | Fermentation treatment method of corn soaking liquid, liquid-phase product obtained by using fermentation treatment method and application of liquid-phase product |
| CN113151380A (en) * | 2021-03-23 | 2021-07-23 | 河北圣雪大成制药有限责任公司 | Culture process for improving titer of nisin |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1280778A (en) * | 2000-08-01 | 2001-01-24 | 冯向法 | Process for preparing lactostreptostacin |
| CN101113463A (en) * | 2007-05-23 | 2008-01-30 | 齐齐哈尔安泰生物工程有限公司 | Preparation method of lactic acid streptostacin |
| CN101906453A (en) * | 2010-08-16 | 2010-12-08 | 环宇格林粮食开发有限公司 | Fermentation process for preparing nisin |
| CN101948893A (en) * | 2010-09-27 | 2011-01-19 | 郑州奇泓生物科技有限公司 | Method for producing nisin by continuous fermentation |
-
2012
- 2012-09-07 CN CN2012103278024A patent/CN102816817A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1280778A (en) * | 2000-08-01 | 2001-01-24 | 冯向法 | Process for preparing lactostreptostacin |
| CN101113463A (en) * | 2007-05-23 | 2008-01-30 | 齐齐哈尔安泰生物工程有限公司 | Preparation method of lactic acid streptostacin |
| CN101906453A (en) * | 2010-08-16 | 2010-12-08 | 环宇格林粮食开发有限公司 | Fermentation process for preparing nisin |
| CN101948893A (en) * | 2010-09-27 | 2011-01-19 | 郑州奇泓生物科技有限公司 | Method for producing nisin by continuous fermentation |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105494978A (en) * | 2015-12-21 | 2016-04-20 | 河南省新大牧业有限公司 | Method for preparing fermented pig feed through corn soaking solution and fermented pig feed prepared through method |
| CN107400688A (en) * | 2017-06-08 | 2017-11-28 | 伟日（山东）生物科技有限公司 | Utilize the method for Wastage of food industry production nisin |
| CN112175999A (en) * | 2019-07-05 | 2021-01-05 | 中粮生物化学（安徽）股份有限公司 | Fermentation treatment method of corn soaking liquid, liquid-phase product obtained by using fermentation treatment method and application of liquid-phase product |
| CN113151380A (en) * | 2021-03-23 | 2021-07-23 | 河北圣雪大成制药有限责任公司 | Culture process for improving titer of nisin |
| CN113151380B (en) * | 2021-03-23 | 2022-11-08 | 河北圣雪大成制药有限责任公司 | Culture process for improving titer of nisin |
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Owner name: JILIN COFCO BIOCHEMICAL CO., LTD. Free format text: FORMER OWNER: JINLIN COFCO BIOCHEMICAL TECHNOLOGY CO., LTD. Effective date: 20131230 |
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