CN106520584B - Saccharomycete and lactic acid bacteria co-culture with culture medium and preparation method thereof - Google Patents
Saccharomycete and lactic acid bacteria co-culture with culture medium and preparation method thereof Download PDFInfo
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- CN106520584B CN106520584B CN201611170235.0A CN201611170235A CN106520584B CN 106520584 B CN106520584 B CN 106520584B CN 201611170235 A CN201611170235 A CN 201611170235A CN 106520584 B CN106520584 B CN 106520584B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a Yeasts and lactic acid bacteria co-cultivation culture medium and preparation method thereof, culture medium includes the component of following parts by weight: 4-6 parts of brown rice, 5-7 parts of brown sugar and 112-132 parts of pure water；Preparation method is the following steps are included: Step 1: by the brown rice of 4-6 parts by weight, and boiling is at steamed brown rice；Step 2: being sterilized after the brown sugar of 5-7 parts by weight to be added to the dissolved in purified water of 2 parts by weight, brown sugar water is made；Step 3: steamed brown rice, brown sugar water and pure water are mixed, culture medium is made.Medium component prepared by the present invention is simply able to satisfy the needs of saccharomycete and lactic acid bacteria breeding again, and stability is good, and at low cost, the holding time is long.
Description
Technical field
The present invention relates to microorganism fields.It is more particularly related to which saccharomycete and lactic acid bacteria co-culture with culture
Base and preparation method thereof.
Background technique
Lactic acid bacteria is a kind of fermentable carbohydrate and the general name of the bacterium that mainly generates a large amount of lactic acid, is usually all leather
Lan Shi positive bacteria and non-pathogenic bacteria.Lactic acid bacteria can not only generate the enzyme for own growth during growth metabolism,
The enzyme that specific function can also be generated can also make in enteron aisle if bifidus factor can not only promote the growth of Bifidobacterium
Beneficial to the value-added special physiological function of mattress group.Lactic acid bacteria metabolism can also generate organic acid, reduce the pH value in gastrointestinal tract, acid
Environment can make pathogen that can not normally grow, be colonized；The hydrogen peroxide of generation can activate catalase, to press down
System and killing catalase positive bacterium (coliform bacteria, Salmonella, pseudomonas etc.)；The bacteriocin of generation can
To inhibit staphylococcus, clostridium, salmonella and the growth of Shigella.In addition to this, lactic acid bacteria also can produce
A variety of metabolites such as biacetyl and lactic acid bacteria itself can generate interferon and antibody with stimulating expression of macrophage, and cell is promoted to exempt from
Epidemic disease promotes the reaction of body nospecific immunity and the reaction of specific immunity, improves the disease resistance of body, and enhancing is immune
Power.In gastrointestinal tract, the lactic acid bacteria of anaerobism can be colonized, and be had actively to adjusting gastrointestinal bacterial flora balance, improving immunity of organisms
Effect.Based on the above physiological function, lactic acid bacteria is applied to fermented feed, the organic acid for being metabolized generation not only changes feed
Smell and palatability, while hydrogen peroxide, the bacteriocin etc. generated can also generate wholesome effect to the body of feeding animals.
Saccharomycete is generally referred to energy fermenting carbohydrate and is carried out a kind of unicellular of vegetative propagation in a manner of budding or fragmentation
Fungi.Yeast cells protein rich in, vitamin and various enzymes etc. are medicine, chemical industry and food fermentation industry
Important source material.Agriculturally, saccharomycete can be used for the production of single cell protein (SCP) and the fermentation of animal feed.So
By saccharomycete be applied to fermented feed in, can not only make feed object can be absorbed using itself generate mycoprotein and
Vitamin, the enzyme that fermentation generates simultaneously can also degrade the anti-nutrition component in feed, and the feed after making fermentation is with more seeking
It nourishs one's nature.
Saccharomycete and lactic acid bacteria possess enzyme system and diversified economy abundant and are worth very high physiological activator, are after animal
Another key protein source after albumen and vegetable protein, fermented product are therefore good feed addictive has
Good value of exploiting and utilizing.Though and the seed culture medium of skimmed milk and each strain currently used for culture yeasts bacterium and lactic acid bacteria
Rich in nutrition content can meet the needs of saccharomycete and lactobacter growth breeding, but since its is at high cost, should not be used to big rule
Mould produces saccharomycete and newborn bacterium.And soybean, corn, also nutriment rich in peanut, it may be used as saccharomycete and cream
The culture medium of sour bacterium.Some vegetables such as carrot, cucumber, tomato etc. also can be used as mixed culture saccharomycete and lactic acid bacteria proliferation is trained
Feeding culture medium, but a large amount of carbon source and nitrogen source need to be added, keep production operation excessively complicated.
Summary of the invention
It is an object of the present invention to provide a Yeasts and lactic acid bacteria co-cultivation culture medium, the simple energy again of ingredient
Meet the needs of saccharomycete and lactic acid bacteria breeding, stability is good, and at low cost, the holding time is long.
It is a further object to provide the preparation method of a Yeasts and lactic acid bacteria co-cultivation culture medium,
It is easy to operate, it is suitable for mass production.
In order to realize these purposes and other advantages according to the present invention, provides a Yeasts and lactic acid bacteria co-cultures
With culture medium, the component including following parts by weight:
4-6 parts of brown rice, 5-7 parts of brown sugar and 112-132 parts of pure water.
One Yeasts and lactic acid bacteria co-culture the preparation method for using culture medium, comprising the following steps:
Step 1: the brown rice of 4-6 parts by weight is cleaned, boiling is spare at steamed brown rice；
Step 2: after the brown sugar of 5-7 parts by weight to be added to the dissolved in purified water of 2 parts by weight, pressure is 0.1MPa, temperature is
Brown sugar water is made in sterilization processing 5min at 121 DEG C, spare；
Step 3: by brown sugar water and 110-130 obtained in steamed brown rice obtained in the step 1, the step 2 weight
The pure water mixing for measuring part, is made culture medium.
The saccharomycete and lactic acid bacteria co-culture in the preparation method with culture medium, after the step 3, further includes:
Step 4: wood vinegar is added in culture medium obtained into step 3 contains 0.1- into every kg broth
After 0.2mL wood vinegar, be uniformly mixed, backward culture medium in the pH value of buffer solution adjusting culture medium is added to 5-6.
The saccharomycete and lactic acid bacteria co-culture in the preparation method with culture medium, and the buffer solution is that pH value is 7
HAc-NaAc buffer solution.
The saccharomycete and lactic acid bacteria co-culture in the preparation method with culture medium, clean brown rice in the step 1
Later, before boiling, further includes:
It is in mass ratio to immerse 10-20 parts by weight after 0.5:1:0.5 is mixed with brown rice, soybean powder after grape is pulverized
In water, backward water in be added the cellulase of 0.1-0.8 parts by weight, the pectase of 0.1-1 parts by weight, 0.1-1.5 parts by weight
Amylase and 0.1-1 parts by weight protease, and adjust pH value to 5-6, digest 2-3h at being 40-55 DEG C in temperature, later
Steamed brown rice is made in boiling 20-30min.
The saccharomycete and lactic acid bacteria co-culture in the preparation method with culture medium, after the step 4, further includes:
Step 5: being in mass ratio that after 4:1:1 is mixed, mixing is made by water-retaining agent, the oyster shell powder of activation and zeolite powder
Powder, it is spare；
Step 6: after the mixed liquor of media surface obtained in step 4 is extracted out with siphon pipe, by 5-30 parts by weight
Mixed-powder be immersed in mixed liquor and be mixed powder to mixed liquor and fully absorb, and water-retaining agent absorption has self weight 200-300
Times mixed liquor after, then by absorb have the mixed-powder of mixed liquor with extraction mixed liquor after culture medium be uniformly mixed.
The saccharomycete and lactic acid bacteria co-culture in the preparation method with culture medium, after the step 6, further includes:
Step 7, the mass fraction for spraying 5-10 parts by weight in culture medium obtained into step 6 under stiring is 20-
30% brown sugar water.
The present invention is include at least the following beneficial effects:
Medium component provided by the invention is simply able to satisfy the needs of saccharomycete and lactic acid bacteria breeding again, and stability is good,
At low cost, the holding time is long.
Contain brown rice, brown sugar, wood vinegar, brown rice starch rich in, protein, rouge in culture medium provided by the invention
Fat, vitamin and 11 kinds of minerals.In brown sugar containing a variety of monosaccharide such as glucose, fructose and polysaccharide energy matter and folic acid,
The substances such as micro substance and amino acid, cellulose are provided rich for saccharomycete and lactic acid bacteria containing organic acids such as cellulose, polysaccharide
Rich carbon source, nitrogen source and other growth factors, meet the growth and breeding needs of saccharomycete and lactic acid bacteria.Edible mushroom is added in wood vinegar
Culture medium in, effect is obviously promoted to the mycelium of edible mushroom, the growth of fructification, can make practical fungus increase production 21-42%.
The present invention digests grape, brown rice and soybean powder, is conducive to cellulose, pectin, starch and protein
It etc. being hydrolyzed to glucose and amino acid, is utilized for saccharomycete and lactic acid bacteria, enzymatic hydrolysis can destroy the cell wall of brown rice and grape, also have
Conducive to the dissolution of intracellular effective component, carbon source, nitrogen source and other growth factors are provided for the breeding of saccharomycete and lactic acid bacteria.
Lactic acid bacteria and saccharomycete are preferably grown in a humid environment, when being cultivated with solid medium, top desiccation
Comparatively fast, and around lactic acid bacteria and saccharomycete a large amount of metabolite is easily accumulated, causes the nutrition distribution pattern of culture medium uneven,
Influence the breeding of lactic acid bacteria and saccharomycete.The present invention joined mixed-powder in the medium, and mixed-powder has very strong suction
It is aqueous, slow release after water in culture medium, wood vinegar, glucose and other growth factors can be absorbed, lactic acid bacteria is continuing to supply
And saccharomycete, so as to improve the yield of lactic acid bacteria and saccharomycete.
Further advantage, target and feature of the invention will be partially reflected by the following instructions, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Specific embodiment
The present invention will be further described in detail below with reference to the embodiments, to enable those skilled in the art referring to specification
Text can be implemented accordingly.
It should be noted that experimental method described in following embodiments is unless otherwise specified conventional method, institute
Reagent and material are stated, unless otherwise specified, is commercially obtained.
Embodiment 1
One Yeasts and lactic acid bacteria, which co-culture, uses culture medium, the component including following parts by weight:
5 parts of brown rice, 6 parts of brown sugar and 122 parts of pure water.
Contain brown rice and brown sugar, brown rice starch rich in, protein, fat, dimension in the culture medium that this programme provides
Raw element and 11 kinds of minerals.Contain a variety of monosaccharide and the polysaccharide energy matters and folic acid, trace content such as glucose, fructose in brown sugar
The substances such as matter and amino acid, cellulose provide carbon abundant containing organic acids such as cellulose, polysaccharide for saccharomycete and lactic acid bacteria
Source, nitrogen source and other growth factors meet the growth and breeding needs of saccharomycete and lactic acid bacteria.
One Yeasts and lactic acid bacteria co-culture the preparation method for using culture medium, comprising the following steps:
Step 1: the brown rice of 5 parts by weight is cleaned, boiling is spare at steamed brown rice；
Step 2: after the brown sugar of 6 parts by weight to be added to the dissolved in purified water of 2 parts by weight, pressure is 0.1MPa, temperature is
Brown sugar water is made in sterilization processing 5min at 121 DEG C, spare；
Step 3: by brown sugar water obtained in steamed brown rice obtained in the step 1, the step 2 and 120 parts by weight
Pure water mixing, be made culture medium.The culture medium is solid-liquid mixed culture medium, and steamed brown rice is immersed in the water.
Embodiment 2
On the basis of embodiment 1, after the step 3, further includes:
Step 4: wood vinegar is added in culture medium obtained into step 3 into every kg broth containing 0.15mL wood
After vinegar liquid, be uniformly mixed, backward culture medium in the pH value of buffer solution adjusting culture medium is added to 5.For saccharomycete and lactic acid
The growth of bacterium, which provides suitable acidity environment, can promote the life of mycelium, fructification by the way that wood vinegar is added in the medium
It is long.
The saccharomycete and lactic acid bacteria co-culture in the preparation method with culture medium, and the buffer solution is that pH value is 7
HAc-NaAc buffer solution.
Embodiment 3
On the basis of embodiment 1, after cleaning brown rice in the step 1, before boiling, further includes:
It is in mass ratio to immerse the water of 15 parts by weight after 0.5:1:0.5 is mixed with brown rice, soybean powder after grape is pulverized
In, backward water in be added the cellulase of 0.4 parts by weight, the pectase of 0.5 parts by weight, 0.12 parts by weight amylase and
The protease of 0.5 parts by weight, and pH value is adjusted to 6,2.5h is digested at being 50 DEG C in temperature, boiling 25min, is made brown rice later
Meal, thermophilic digestion can be such that enzyme inactivates, such enzyme will not hydrolyzed yeast bacterium cell wall.Grape, brown rice and soybean powder are carried out
Enzymatic hydrolysis, enzymatic hydrolysis can destroy the cell wall of brown rice and grape, be conducive to cellulose, pectin, starch and protein etc. being hydrolyzed to Portugal
Grape sugar and amino acid, directly utilize for saccharomycete and lactic acid bacteria, by destroying the cell wall of brown rice and grape, are also beneficial to cell
The dissolution of interior effective component provides carbon source, nitrogen source and other growth factors for the breeding of saccharomycete and lactic acid bacteria.
Embodiment 4
On the basis of embodiment 2, after the step 4, further includes:
Step 5: being in mass ratio that after 4:1:1 is mixed, mixing is made by water-retaining agent, the oyster shell powder of activation and zeolite powder
Powder, it is spare；Mixed-powder has extremely strong water imbibition, after capable of absorbing the effective components such as water, wood vinegar, amino acid, glucose
Slow release had not only been able to maintain the humidity of culture medium, but can be provided for the growth of lactic acid bacteria and saccharomycete carbon source, nitrogen source and growth because
Son.
Step 6: after the mixed liquor of media surface obtained in step 4 is extracted out with siphon pipe, by 10 parts by weight
Mixed-powder, which is immersed in mixed liquor, to be mixed powder to mixed liquor and fully absorbs, and water-retaining agent absorbs the mixed of 300 times of self weight
Close liquid after, then by absorb have the mixed-powder of mixed liquor with extraction mixed liquor after culture medium be uniformly mixed.The culture medium is solid
Body culture medium, but humidity is larger, and myopia is in fluid nutrient medium.
The saccharomycete and lactic acid bacteria co-culture in the preparation method with culture medium, after the step 6, further includes:
Step 7, the mass fraction for spraying 6 parts by weight in culture medium obtained into step 6 under stiring is 25%
Brown sugar water.The surface that steamed brown rice, mixed-powder etc. are further soaked by brown sugar water, keeps the humidity in culture medium bigger, and make
Mixed-powder absorbs more moisture until can not be further continued for absorbing moisture, such mixed-powder will not absorb steamed brown rice surface
Moisture slowly releases when the moisture content of steamed brown rice is lower, then by moisture and other compositions.
Embodiment 5
One Yeasts and lactic acid bacteria, which co-culture, uses culture medium, the component including following parts by weight:
4 parts of brown rice, 5 parts of brown sugar and 112 parts of pure water.
One Yeasts and lactic acid bacteria co-culture the preparation method for using culture medium, comprising the following steps:
Step 1: the brown rice of 4 parts by weight is cleaned, boiling is spare at steamed brown rice；
Step 2: after the brown sugar of 5 parts by weight to be added to the dissolved in purified water of 2 parts by weight, pressure is 0.1MPa, temperature is
Brown sugar water is made in sterilization processing 5min at 121 DEG C, spare；
Step 3: by brown sugar water obtained in steamed brown rice obtained in the step 1, the step 2 and 110 parts by weight
Pure water mixing, be made culture medium.
The saccharomycete and lactic acid bacteria co-culture in the preparation method with culture medium, after the step 3, further includes:
Step 4: wood vinegar is added in culture medium obtained into step 3 into every kg broth containing 0.1mL wood
After vinegar liquid, be uniformly mixed, backward culture medium in the pH value of buffer solution adjusting culture medium is added to 5.
The saccharomycete and lactic acid bacteria co-culture in the preparation method with culture medium, and the buffer solution is that pH value is 7
HAc-NaAc buffer solution.
The saccharomycete and lactic acid bacteria co-culture in the preparation method with culture medium, clean brown rice in the step 1
Later, before boiling, further includes:
It is in mass ratio to immerse the water of 10 parts by weight after 0.5:1:0.5 is mixed with brown rice, soybean powder after grape is pulverized
In, backward water in be added the cellulase of 0.1 parts by weight, the pectase of 0.1 parts by weight, 0.1 parts by weight amylase and 0.1
The protease of parts by weight, and pH value is adjusted to 5,2h is digested at being 40 DEG C in temperature, boiling 20min, is made steamed brown rice later.
The saccharomycete and lactic acid bacteria co-culture in the preparation method with culture medium, after the step 4, further includes:
Step 5: being in mass ratio that after 4:1:1 is mixed, mixing is made by water-retaining agent, the oyster shell powder of activation and zeolite powder
Powder, it is spare；
Step 6: after the mixed liquor of media surface obtained in step 4 is extracted out with siphon pipe, by the mixed of 5 parts by weight
It closes powder and is immersed in mixed liquor and be mixed powder to mixed liquor and fully absorb, and water-retaining agent absorbs the mixing for having 200 times of self weight
After liquid, then by absorb have the mixed-powder of mixed liquor with extraction mixed liquor after culture medium be uniformly mixed.
The saccharomycete and lactic acid bacteria co-culture in the preparation method with culture medium, after the step 6, further includes:
Step 7, the mass fraction for spraying 5 parts by weight in culture medium obtained into step 6 under stiring is 20%
Brown sugar water.
Embodiment 6
One Yeasts and lactic acid bacteria, which co-culture, uses culture medium, the component including following parts by weight:
6 parts of brown rice, 7 parts of brown sugar and 132 parts of pure water.
One Yeasts and lactic acid bacteria co-culture the preparation method for using culture medium, comprising the following steps:
Step 1: the brown rice of 6 parts by weight is cleaned, boiling is spare at steamed brown rice；
Step 2: after the brown sugar of 7 parts by weight to be added to the dissolved in purified water of 2 parts by weight, pressure is 0.1MPa, temperature is
Brown sugar water is made in sterilization processing 5min at 121 DEG C, spare；
Step 3: by brown sugar water obtained in steamed brown rice obtained in the step 1, the step 2 and 130 parts by weight
Pure water mixing, be made culture medium.
The saccharomycete and lactic acid bacteria co-culture in the preparation method with culture medium, after the step 3, further includes:
Step 4: wood vinegar is added in culture medium obtained into step 3 into every kg broth containing 0.2mL wood
After vinegar liquid, be uniformly mixed, backward culture medium in the pH value of buffer solution adjusting culture medium is added to 6.
The saccharomycete and lactic acid bacteria co-culture in the preparation method with culture medium, and the buffer solution is that pH value is 7
HAc-NaAc buffer solution.
The saccharomycete and lactic acid bacteria co-culture in the preparation method with culture medium, clean brown rice in the step 1
Later, before boiling, further includes:
It is in mass ratio to immerse the water of 20 parts by weight after 0.5:1:0.5 is mixed with brown rice, soybean powder after grape is pulverized
In, backward water in the cellulase of 0.8 parts by weight, the pectase of 1 parts by weight, the amylase of 1.5 parts by weight and 1 weight is added
The protease of part, and pH value is adjusted to 6,3h is digested at being 55 DEG C in temperature, boiling 30min, is made steamed brown rice later.
The saccharomycete and lactic acid bacteria co-culture in the preparation method with culture medium, after the step 4, further includes:
Step 5: being in mass ratio that after 4:1:1 is mixed, mixing is made by water-retaining agent, the oyster shell powder of activation and zeolite powder
Powder, it is spare；
Step 6: after the mixed liquor of media surface obtained in step 4 is extracted out with siphon pipe, by 30 parts by weight
Mixed-powder, which is immersed in mixed liquor, to be mixed powder to mixed liquor and fully absorbs, and water-retaining agent absorbs the mixed of 300 times of self weight
Close liquid after, then by absorb have the mixed-powder of mixed liquor with extraction mixed liquor after culture medium be uniformly mixed.
The saccharomycete and lactic acid bacteria co-culture in the preparation method with culture medium, after the step 6, further includes:
Step 7, the mass fraction for spraying 10 parts by weight in culture medium obtained into step 6 under stiring is 30%
Brown sugar water.
Experiment 1
By III bacterial strain of lactobacillus plantarum (Lb.plantarum) ST-, (CGMCC NO.0847 is studied purchased from bright milk industry
Basic research portion, institute) MRS culture medium (control group) and the present invention implementation of commercialization are respectively connected to the inoculum concentration of 1% (V/V)
In the culture medium of example 1-6, then brewer's yeast (AS2.4) is respectively connected to the MRS being commercialized with the inoculum concentration of 1% (V/V) and is cultivated
In the culture medium of base and 1-6 of the embodiment of the present invention, 35 DEG C of Anaerobic culturels measure Bacillus acidi lactici and saccharomycete for 24 hours and after 48h respectively
Total plate count, the results are shown in Table 1.
Table 1 is using the clump count after the culture medium culture of control group and embodiment 1-6
As can be seen from Table 1, lactic acid bacteria and saccharomycete are cultivated using culture medium prepared by 1-6 of the embodiment of the present invention
Afterwards, clump count is all larger than the clump count cultivated using MRS culture medium, illustrates to promote using culture medium prepared by the present invention
The growth of lactic acid bacteria and saccharomycete, so as to improve the yield of lactic acid bacteria and saccharomycete.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and embodiment shown and described herein.
Claims (3)
1. a Yeasts and lactic acid bacteria co-culture the preparation method for using culture medium, which comprises the following steps:
Step 1: the brown rice of 4-6 parts by weight is cleaned, boiling is spare at steamed brown rice；
Step 2: after the brown sugar of 5-7 parts by weight to be added to the dissolved in purified water of 2 parts by weight, pressure be 0.1MPa, temperature 121
Brown sugar water is made in sterilization processing 5min at DEG C, spare；
Step 3: by brown sugar water and 110-130 parts by weight obtained in steamed brown rice obtained in the step 1, the step 2
Pure water mixing, be made culture medium；
Step 4: wood vinegar is added in culture medium obtained into step 3 into every kg broth containing 0.1-0.2mL wood
After vinegar liquid, be uniformly mixed, backward culture medium in the pH value of buffer solution adjusting culture medium is added to 5-6；
Step 5: be in mass ratio that after 4:1:1 is mixed, mixed-powder is made by water-retaining agent, the oyster shell powder of activation and zeolite powder,
It is spare；
Step 6: after the mixed liquor of media surface obtained in step 4 is extracted out with siphon pipe, by the mixed of 5-30 parts by weight
It closes powder and is immersed in mixed liquor and be mixed powder to mixed liquor and fully absorb, and water-retaining agent absorbs 200-300 times of self weight
After mixed liquor, then by absorb have the mixed-powder of mixed liquor with extraction mixed liquor after culture medium be uniformly mixed；
Step 7, the mass fraction for spraying 5-10 parts by weight in culture medium obtained into step 6 under stiring is 20-30%
Brown sugar water.
2. saccharomycete as described in claim 1 and lactic acid bacteria co-culture the preparation method for using culture medium, which is characterized in that described
Buffer solution is the HAc-NaAc buffer solution that pH value is 7.
3. saccharomycete as described in claim 1 and lactic acid bacteria co-culture the preparation method for using culture medium, which is characterized in that described
After cleaning brown rice in step 1, before boiling, further includes:
It is in mass ratio to immerse in the water of 10-20 parts by weight after 0.5:1:0.5 is mixed with brown rice, soybean powder after grape is pulverized,
Backward water in be added the cellulase of 0.1-0.8 parts by weight, the pectase of 0.1-1 parts by weight, 0.1-1.5 parts by weight starch
The protease of enzyme and 0.1-1 parts by weight, and pH value is adjusted to 5-6,2-3h is digested at being 40-55 DEG C in temperature, later boiling 20-
Steamed brown rice is made in 30min.
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| CN107746823A (en) * | 2017-11-29 | 2018-03-02 | 广西康佳龙农牧集团有限公司 | It is enriched with solid-state symbiotic culture medium of lactic acid bacteria and saccharomycete and preparation method thereof |
| CN109112175B (en) * | 2018-09-14 | 2021-01-29 | 江南大学 | Method for constructing microbial co-culture system to produce pleurotus tuber-regium exopolysaccharide at high yield |
| CN109370933B (en) * | 2018-09-28 | 2021-11-02 | 四川大学 | Co-culture method for improving acid resistance of saccharomycetes |
| CN110304952A (en) * | 2019-06-18 | 2019-10-08 | 伊春林业科学院 | A kind of Submerged Culture of Morchella conica base and preparation method thereof adding wood vinegar |
| US11597950B1 (en) | 2020-03-11 | 2023-03-07 | Poet Research, Inc. | Systems, compositions, and methods of fermentation with Z. mobilis |
| US11732278B1 (en) | 2020-03-11 | 2023-08-22 | Poet Research, Inc. | Systems and methods for co-culture of oxygen sensitive bacteria and yeast |
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| "日本对大米食品的开发";周秀琴;《粮食与油脂》;19891231(第4期);第16-19页 * |
| 酵母生产工艺操作;凌生才;《酿酒科技》;20021231(第4期);第37、36页 * |
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