CN101756011A - Method for utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff - Google Patents
Method for utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff Download PDFInfo
- Publication number
- CN101756011A CN101756011A CN200810152163A CN200810152163A CN101756011A CN 101756011 A CN101756011 A CN 101756011A CN 200810152163 A CN200810152163 A CN 200810152163A CN 200810152163 A CN200810152163 A CN 200810152163A CN 101756011 A CN101756011 A CN 101756011A
- Authority
- CN
- China
- Prior art keywords
- lysine
- culture medium
- quality
- strains
- solid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
Abstract
The invention discloses a method for utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff. The method incudes the following steps of (1) preparing a lysine seed culture medium, and transplanting corynebacterium glutmicum strains into the lysine seed culture medium for culturing, (2) preparing a potato culture medium, and transplanting aspergillus niger strains into the potato culture medium for culturing, and (3) transplanting corynebacterium glutmicum strains solution and aspergillus niger strains solution into a solid fermented culture medium containing the sauce/vinegar dregs for fermentation; drying is carried out under the temperature of not higher than 85 DEG C till the water content is lower than 15%, so as to obtain the multi-enzyme high-lysine feedstuff produced by the dual-strains solid fermented sauce/vinegar dregs. The feedstuff produced by the method in the invention contains large quantity of microbe living cells, microbe enzymes, lysine and the like which have significant functions of promoting digestive absorption, growing development as well as organism immunity of animals, increasing beneficial bacterial groups in intestinal tracts of the animals, and improving the intestinal micro-ecology and the like.
Description
Technical field
The present invention relates to a kind of solid microbe fermentation technology, particularly relate to a kind of method of utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff.
Background technology
Soy sauce slag and acid-sludge can be directly as animal feeds, and these feeds usually are directly to feed various animals through processing backs such as simple pulverizing, boilings.The practice makes the utilization rate of active components in the feed low like this, has caused a large amount of wastes.And for a long time edible such feed of animal, can cause malnutrition, hypoimmunity.Slow down the speed of growth of animal or reduce the laying rate of bird.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of method of utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff is provided, the feed that utilizes method of the present invention to produce can improve the effective rate of utilization of feed, improve effective output capacity of letting animals feed, reduce the letting animals feed incidence of disease.
Technical scheme of the present invention is summarized as follows:
A kind of method of utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff, form by following step:
(1) preparation lysine seed culture medium; In the lysine seed culture medium, make described Corynebacterium glutamicum bacterial classification account for the 1%-3% of lysine seed culture medium quality the Corynebacterium glutamicum bacterial classification inoculation, 25-35 ℃, under the condition of ventilating, cultivated 18-48 hour;
(2) the preparation potato culture is inoculated into aspergillus niger strain in the potato culture, makes described aspergillus niger strain account for the 1%-3% of potato culture quality, 25-35 ℃, cultivates 18-48 hour under the condition of ventilating;
(3) will be inoculated in the solid-state fermentation culture medium that contains soy sauce slag and acid-sludge through the Corynebacterium glutamicum strain liquid of step (1) cultivation maturation with through the ripe aspergillus niger strain liquid of step (2) cultivation, described Corynebacterium glutamicum strain liquid accounts for the 3%-10% of solid-state fermentation culture medium quality, the inoculation of aspergillus niger strain liquid accounts for the 5%-15% of solid-state fermentation culture medium quality, the water quality that contains of control solid-state fermentation culture medium is 40%-60%, at 25-35 ℃, ventilate, fermented 70-80 hour; Be dried to water content less than 15% not being higher than under 85 ℃ the temperature, make a kind of utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff.
Described lysine seed culture medium is to make with following method: take by weighing glucose 2.0%, (NH by quality
4)
2SO
40.4%, urea 0.1%, K
2HPO
40.1%, corn steep liquor 2%, soya-bean cake hydrolase 2%, MgSO
40.025%, surplus is a water, and regulating pH is 7.0, at 0.1Mpa sterilization 15min.
Described potato culture is to make with following method: take by weighing potato 20%% by quality, and sucrose 2%, surplus is a water, sterilization treatment 10-15min under 121 ℃ of temperature.
Described solid-state fermentation culture medium is to make with following method: take by weighing soy sauce slag 40%-60%, acid-sludge 5%-15%, wheat bran 30%-50% by quality; With the soy sauce slag, acid-sludge and wheat bran gross weight are 100% for benchmark, add molasses 12%, corn steep liquor 2%, soya-bean cake hydrolyzate 8%, ammonium sulfate 2.5%, urea 1.25%, dipotassium hydrogen phosphate 0.10%, potassium dihydrogen phosphate 0.10%, magnesium sulfate 0.025%, quick lime 1%, water content 70%, mixing.
Advantage of the present invention is:
The present invention accomplishes comprehensive utilization of waste materials, improves added value of product.By containing a large amount of micro-organisms living cells, microbial enzymes and vitamin etc. in the feed of method processing of the present invention back.These materials to the digesting and assimilating of animal, grow, immunity of organism, increase profitable strain in the animal intestinal, improve intestinal microecology etc. and all have tangible effect.Protein content after method of the present invention is handled in the product can improve more than 10%, and essential amino acid can increase more than 1 times, and lysine improves 5.0 times, and the content of lysine is up to the 8.37g/100g dry feed.The product that method of the present invention is made adds in the animal feed, gives this feed of animal feeding, can increase disease resistance of animals, promotes fast growth of animal, reduces feedstuff-meat ratio, improves the quality of animal flesh, improves the egg production of chicken.
The feed that adopts method of the present invention to produce can be produced healthy and safe food such as antibiotic-free milk, nonreactive pork.The present invention still solves the important channel of protein resource deficiency.Breed by microbial fermentation, a large amount of somatic cells, synthesized a large amount of mattress body proteins and useful metabolite simultaneously, these mycoproteins all are the forage proteins of high-quality.Primary raw material of the present invention is wheat bran, soy sauce slag and acid-sludge, and is cheap and easy to get, improves its nutritive value and economic worth greatly after fermentation.
The specific embodiment
The present invention is further illustrated below by specific embodiment.
Embodiment 1
A kind of method of utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff, form by following step:
(1) preparation lysine seed culture medium takes by weighing glucose 2.0%, (NH by quality
4)
2SO
40.4%, urea 0.1%, K
2HPO
40.1%, corn steep liquor 2%, soya-bean cake hydrolase 2%, MgSO
40.025%, surplus is a water, and regulating pH is 7.0, at 0.1Mpa sterilization 15min; In the lysine seed culture medium, make described Corynebacterium glutamicum bacterial classification account for 2%, 30 ℃ of lysine seed culture medium quality the Corynebacterium glutamicum bacterial classification inoculation, under the condition of ventilating, cultivated 30 hours;
(2) the preparation potato culture takes by weighing potato 20% by quality, sucrose 2%, and surplus is a water, sterilization treatment 13min under 121 ℃ of temperature; Aspergillus niger strain is inoculated in the potato culture, makes described aspergillus niger strain account for 2%, 28 ℃ of potato culture quality, under the condition of ventilating, cultivated 32 hours;
(3) the preparation solid-state fermentation culture medium takes by weighing soy sauce slag 50% by quality, acid-sludge 10%, wheat bran 40%; With the soy sauce slag, acid-sludge and wheat bran gross weight are 100% for benchmark, add molasses 12%, corn steep liquor 2%, soya-bean cake hydrolyzate 8%, ammonium sulfate 2.5%, urea 1.25%, dipotassium hydrogen phosphate 0.10%, potassium dihydrogen phosphate 0.10%, magnesium sulfate 0.025%, quick lime 1%, water content 70%, mixing; To cultivate ripe Corynebacterium glutamicum strain liquid and cultivate ripe aspergillus niger strain liquid through step (1) and be inoculated in the solid-state fermentation culture medium that contains soy sauce slag and acid-sludge through step (2), described Corynebacterium glutamicum strain liquid accounts for 7% of solid-state fermentation culture medium quality, the inoculation of aspergillus niger strain liquid accounts for 10% of solid-state fermentation culture medium quality, the water quality that contains of control solid-state fermentation culture medium is 50%, at 30 ℃, ventilate, fermented 75 hours; Be dried to water content less than 15% not being higher than under 85 ℃ the temperature, make a kind of utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff.The feed of preparation contains lysine 6.4%, zytase content 〉=414000U/g, 1,4 beta-glucanase 〉=38000U/g and cellulase 〉=55040U/g, and probio content is greater than 4.7X10
9/ g.
Embodiment 2
A kind of method of utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff, form by following step:
(1) preparation lysine seed culture medium takes by weighing glucose 2.0%, (NH by quality
4)
2SO
40.4%, urea 0.1%, K
2HPO
40.1%, corn steep liquor 2%, soya-bean cake hydrolase 2%, MgSO
40.025%, surplus is a water, and regulating pH is 7.0, at 0.1Mpa sterilization 15min; In the lysine seed culture medium, make described Corynebacterium glutamicum bacterial classification account for 1%, 25 ℃ of lysine seed culture medium quality the Corynebacterium glutamicum bacterial classification inoculation, under the condition of ventilating, cultivated 48 hours;
(2) the preparation potato culture takes by weighing potato 20%% by quality, sucrose 2%, and surplus is a water, sterilization treatment 12min under 121 ℃ of temperature; Aspergillus niger strain is inoculated in the potato culture, makes described aspergillus niger strain account for 1%, 35 ℃ of potato culture quality, under the condition of ventilating, cultivated 18 hours;
(3) the preparation solid-state fermentation culture medium takes by weighing soy sauce slag 40% by quality, acid-sludge 15%, wheat bran 45%; With the soy sauce slag, acid-sludge and wheat bran gross weight are 100% for benchmark, add molasses 12%, corn steep liquor 2%, soya-bean cake hydrolyzate 8%, ammonium sulfate 2.5%, urea 1.25%, dipotassium hydrogen phosphate 0.10%, potassium dihydrogen phosphate 0.10%, magnesium sulfate 0.025%, quick lime 1%, water content 70%, mixing; To cultivate ripe Corynebacterium glutamicum strain liquid and cultivate ripe aspergillus niger strain liquid through step (1) and be inoculated in the solid-state fermentation culture medium that contains soy sauce slag and acid-sludge through step (2), described Corynebacterium glutamicum strain liquid accounts for 3% of solid-state fermentation culture medium quality, the inoculation of aspergillus niger strain liquid accounts for 15% of solid-state fermentation culture medium quality, the water quality that contains of control solid-state fermentation culture medium is 60%, at 25 ℃, ventilate, fermented 80 hours; Be dried to water content less than 15% not being higher than under 85 ℃ the temperature, make a kind of utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff.The feed of preparation contains protein lysine 5.2%, zytase content 〉=65000U/g, 1,4 beta-glucanase 〉=98000U/g and cellulase 〉=68040U/g, and probio content is greater than 1.1X10
9/ g.
Embodiment 3
A kind of method of utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff, form by following step:
(1) preparation lysine seed culture medium takes by weighing glucose 2.0%, (NH by quality
4)
2SO
40.4%, urea 0.1%, K
2HPO
40.1%, corn steep liquor 2%, soya-bean cake hydrolase 2%, MgSO
40.025%, surplus is a water, and regulating pH is 7.0, at 0.1Mpa sterilization 15min; In the lysine seed culture medium, make described Corynebacterium glutamicum bacterial classification account for 3%, 35 ℃ of lysine seed culture medium quality the Corynebacterium glutamicum bacterial classification inoculation, under the condition of ventilating, cultivated 18 hours;
(2) the preparation potato culture takes by weighing potato 20%% by quality, sucrose 2%, and surplus is a water, sterilization treatment 10min under 121 ℃ of temperature; Aspergillus niger strain is inoculated in the potato culture, makes described aspergillus niger strain account for 3%, 25 ℃ of potato culture quality, under the condition of ventilating, cultivated 48 hours;
(3) the preparation solid-state fermentation culture medium takes by weighing soy sauce slag 45% by quality, acid-sludge 5%, wheat bran 50%; With the soy sauce slag, acid-sludge and wheat bran gross weight are 100% for benchmark, add molasses 12%, corn steep liquor 2%, soya-bean cake hydrolyzate 8%, ammonium sulfate 2.5%, urea 1.25%, dipotassium hydrogen phosphate 0.10%, potassium dihydrogen phosphate 0.10%, magnesium sulfate 0.025%, quick lime 1%, water content 70%, mixing; To cultivate ripe Corynebacterium glutamicum strain liquid and cultivate ripe aspergillus niger strain liquid through step (1) and be inoculated in the solid-state fermentation culture medium that contains soy sauce slag and acid-sludge through step (2), described Corynebacterium glutamicum strain liquid accounts for 10% of solid-state fermentation culture medium quality, the inoculation of aspergillus niger strain liquid accounts for 5% of solid-state fermentation culture medium quality, the water quality that contains of control solid-state fermentation culture medium is 40%, at 35 ℃, ventilate, fermented 70 hours; Be dried to water content less than 15% not being higher than under 85 ℃ the temperature, make a kind of utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff.
Embodiment 4
A kind of method of utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff, form by following step:
(1) preparation lysine seed culture medium takes by weighing glucose 2.0%, (NH by quality
4)
2SO
40.4%, urea 0.1%, K
2HPO
40.1%, corn steep liquor 2%, soya-bean cake hydrolase 2%, MgSO
40.025%, surplus is a water, and regulating pH is 7.0, at 0.1Mpa sterilization 15min; In the lysine seed culture medium, make described Corynebacterium glutamicum bacterial classification account for 2%, 32 ℃ of lysine seed culture medium quality the Corynebacterium glutamicum bacterial classification inoculation, under the condition of ventilating, cultivated 20 hours;
(2) the preparation potato culture takes by weighing potato 20%% by quality, sucrose 2%, and surplus is a water, sterilization treatment 15min under 121 ℃ of temperature; Aspergillus niger strain is inoculated in the potato culture, makes described aspergillus niger strain account for 2%, 30 ℃ of potato culture quality, under the condition of ventilating, cultivated 30 hours;
(3) the preparation solid-state fermentation culture medium takes by weighing soy sauce slag 60% by quality, acid-sludge 10%, wheat bran 30%; With the soy sauce slag, acid-sludge and wheat bran gross weight are 100% for benchmark, add molasses 12%, corn steep liquor 2%, soya-bean cake hydrolyzate 8%, ammonium sulfate 2.5%, urea 1.25%, dipotassium hydrogen phosphate 0.10%, potassium dihydrogen phosphate 0.10%, magnesium sulfate 0.025%, quick lime 1%, water content 70%, mixing; To cultivate ripe Corynebacterium glutamicum strain liquid and cultivate ripe aspergillus niger strain liquid through step (1) and be inoculated in the solid-state fermentation culture medium that contains soy sauce slag and acid-sludge through step (2), described Corynebacterium glutamicum strain liquid accounts for 5% of solid-state fermentation culture medium quality, the inoculation of aspergillus niger strain liquid accounts for 8% of solid-state fermentation culture medium quality, the water quality that contains of control solid-state fermentation culture medium is 50%, at 28 ℃, ventilate, fermented 72 hours; Be dried to water content less than 15% not being higher than under 85 ℃ the temperature, make a kind of utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff.
Utilize the multi-enzyme high-lysine feedstuff of method preparation of the present invention, meet GB 13078.2001 forage health standards.Wherein: the feed of embodiment 1 preparation contains protein 22.42%, carbohydrate 24.45%, crude fibre 6.8%, lysine 5.7%, zytase content 〉=24000U/g, 1,4 beta-glucanase 〉=36000U/g and cellulase 〉=50040U/g, probio content is greater than 8.5X10
9/ g.The product good palatability, digestibility height, nutritional labeling general equilibrium.
Effect
The feed of embodiment 1 preparation of interpolation 5% in laying ration, egg production improves 9.8%; Efficiency of feed utilization improves 14.1%, and egg size improves 3.2%, and rate of fertilization improves 1.2%, and yolk color is darker.
Add the feed of 3% embodiment, 2 preparations in daily ration of broiler, increase day by day and bring up again 9.1%, the price of deed improves 8.5%, and Fresh ﹠ Tender in Texture good to eat.
The feed of embodiment 3 preparations of interpolation 2% in the chick daily ration, survival rate, rate of body weight gain and feed conversion rate then improve 8.1%, 11.5% and 23.4% respectively, shorten 11 days growth period.
The present invention accomplishes comprehensive utilization of waste materials, improves added value of product.By containing a large amount of micro-organisms living cells, microbial enzymes and vitamin etc. in the feed of method processing of the present invention back.These materials to the digesting and assimilating of animal, grow, immunity of organism, increase profitable strain in the animal intestinal, improve intestinal microecology etc. and all have tangible effect.Protein content after method of the present invention is handled in the product can improve more than 10%, and essential amino acid can increase more than 1 times, and lysine content improves 5-10%, and the content of lysine is up to the 8.37g/100g dry feed.The product that method of the present invention is made adds in the animal feed, gives this feed of animal feeding, can increase disease resistance of animals, promotes fast growth of animal, reduces feedstuff-meat ratio, improves the quality of animal flesh, improves the egg production of chicken.
Claims (4)
1. the method for a utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff is characterized in that being made up of following step:
(1) preparation lysine seed culture medium; In the lysine seed culture medium, make described Corynebacterium glutamicum bacterial classification account for the 1%-3% of lysine seed culture medium quality the Corynebacterium glutamicum bacterial classification inoculation, 25-35 ℃, under the condition of ventilating, cultivated 18-48 hour;
(2) the preparation potato culture is inoculated into aspergillus niger strain in the potato culture, makes described aspergillus niger strain account for the 1%-3% of potato culture quality, 25-35 ℃, cultivates 18-48 hour under the condition of ventilating;
(3) will be inoculated in the solid-state fermentation culture medium that contains soy sauce slag and acid-sludge through the Corynebacterium glutamicum strain liquid of step (1) cultivation maturation with through the ripe aspergillus niger strain liquid of step (2) cultivation, described Corynebacterium glutamicum strain liquid accounts for the 3%-10% of solid-state fermentation culture medium quality, the inoculation of aspergillus niger strain liquid accounts for the 5%-15% of solid-state fermentation culture medium quality, the water quality that contains of control solid-state fermentation culture medium is 40%-60%, at 25-35 ℃, ventilate, fermented 70-80 hour; Be dried to water content less than 15% not being higher than under 85 ℃ the temperature, make a kind of utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff.
2. the method for a kind of utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff according to claim 1 is characterized in that described lysine seed culture medium is to make with following method: take by weighing glucose 2.0%, (NH by quality
4)
2SO
40.4%, urea 0.1%, K
2HPO
40.1%, corn steep liquor 2%, soya-bean cake hydrolase 2%, MgSO
40.025%, surplus is a water, and regulating pH is 7.0, at 0.1Mpa sterilization 15min.
3. the method for a kind of utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff according to claim 1, it is characterized in that described potato culture is to make with following method: take by weighing potato 20% by quality, sucrose 2%, surplus is a water, sterilization treatment 10-15min under 121 ℃ of temperature.
4. the method for a kind of utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff according to claim 1, it is characterized in that described solid-state fermentation culture medium is to make with following method: take by weighing soy sauce slag 40%-60% by quality, acid-sludge 5%-15%, wheat bran 30%-50%; With the soy sauce slag, acid-sludge and wheat bran gross weight are 100% for benchmark, add molasses 12%, corn steep liquor 2%, soya-bean cake hydrolyzate 8%, ammonium sulfate 2.5%, urea 1.25%, dipotassium hydrogen phosphate 0.10%, potassium dihydrogen phosphate 0.10%, magnesium sulfate 0.025%, quick lime 1%, water content 70%, mixing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810152163A CN101756011A (en) | 2008-10-08 | 2008-10-08 | Method for utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810152163A CN101756011A (en) | 2008-10-08 | 2008-10-08 | Method for utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101756011A true CN101756011A (en) | 2010-06-30 |
Family
ID=42487517
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200810152163A Pending CN101756011A (en) | 2008-10-08 | 2008-10-08 | Method for utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101756011A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102524544A (en) * | 2012-01-06 | 2012-07-04 | 广东美味鲜调味食品有限公司 | Feed enzymic preparation prepared by using soy sauce residues and preparation method of feed enzymic preparation |
| CN102630808A (en) * | 2012-04-18 | 2012-08-15 | 华南理工大学 | Method for processing soy sauce residue into feed major ingredient |
| WO2013076686A3 (en) * | 2011-11-23 | 2013-07-18 | Universidad De Antioquia | Functional unlimited culture medium having a natural biocomposite inducer |
| CN111838407A (en) * | 2020-07-15 | 2020-10-30 | 山东理工大学 | High-protein probiotic feed prepared from sauce residue by solid state fermentation method and preparation method thereof |
| CN113519691A (en) * | 2021-05-27 | 2021-10-22 | 通辽市海邻生物科技有限公司 | Preparation method of high-lysine biological feed |
-
2008
- 2008-10-08 CN CN200810152163A patent/CN101756011A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013076686A3 (en) * | 2011-11-23 | 2013-07-18 | Universidad De Antioquia | Functional unlimited culture medium having a natural biocomposite inducer |
| CN102524544A (en) * | 2012-01-06 | 2012-07-04 | 广东美味鲜调味食品有限公司 | Feed enzymic preparation prepared by using soy sauce residues and preparation method of feed enzymic preparation |
| CN102630808A (en) * | 2012-04-18 | 2012-08-15 | 华南理工大学 | Method for processing soy sauce residue into feed major ingredient |
| CN111838407A (en) * | 2020-07-15 | 2020-10-30 | 山东理工大学 | High-protein probiotic feed prepared from sauce residue by solid state fermentation method and preparation method thereof |
| CN113519691A (en) * | 2021-05-27 | 2021-10-22 | 通辽市海邻生物科技有限公司 | Preparation method of high-lysine biological feed |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102599351B (en) | Bio-enzyme and microorganism containing compound feed additive and preparation process thereof | |
| CN104186957A (en) | Method for improving forage nutritional value of cottonseed meal through microbial fermentation | |
| CN101305769B (en) | Preparation method of microbial fermentative feedstuff | |
| CN104920806A (en) | Bran protein feed preparing method by using mixed bacteria multi-step fermentation | |
| CN110150458A (en) | A method of forage protein is prepared using microbial fermentation black soldier flies larva | |
| CN104222668A (en) | Compound fish feed capable of improving body immunity | |
| CN101756012A (en) | Method for producing high-lysine fermented feed through fermentation of vinegar residue using two-step method | |
| CN104542461A (en) | Breeding method for Laiwu pigs | |
| CN104106727A (en) | Complete fermented feed and preparing method thereof | |
| CN106212915B (en) | The method and product of staged fermentation production cattle and sheep complete feed | |
| CN103385350B (en) | Soft particle milk replacer containing high fructose corn syrup and preparation thereof | |
| CN103704472B (en) | Functional concentrated feed of a kind of FOS and preparation method thereof | |
| CN103564199A (en) | Method for producing cow feed by multi-strain mixing solid-state fermentation | |
| CN101120719B (en) | Microorganism ferment purple sweet potato and preparation method thereof | |
| CN103829042A (en) | Production method of multi-vitamin active cassava protein feed | |
| CN104855680A (en) | Pleurotus nebrodensis fungus chaff fermented feed and processing technology thereof | |
| CN106234755B (en) | The method for producing cattle and sheep complete feed as raw material staged fermentation using bagasse | |
| CN103704501B (en) | Method for producing high-efficiency broiler feed by utilization of corn straw fermentation | |
| CN106260589A (en) | A kind of method that recycling wheat bran prepares albumen feedstuff | |
| CN108077616A (en) | A kind of biological straw fermented feed preparation method for fattening period live pig | |
| CN108432979A (en) | A kind of Australia freshwater lobster ecological feed | |
| CN101361520A (en) | Potato pulp energy fermentation feed capable of replacing bran and preparation method thereof | |
| CN101756011A (en) | Method for utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff | |
| CN110447456A (en) | A kind of selenium-enriched edible mushroom production technology | |
| CN103859149A (en) | Production technology of lactobacillus fermented soybean meal |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20100630 |