CN107400688A - Utilize the method for Wastage of food industry production nisin - Google Patents
Utilize the method for Wastage of food industry production nisin Download PDFInfo
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- CN107400688A CN107400688A CN201710425658.0A CN201710425658A CN107400688A CN 107400688 A CN107400688 A CN 107400688A CN 201710425658 A CN201710425658 A CN 201710425658A CN 107400688 A CN107400688 A CN 107400688A
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- 108010053775 Nisin Proteins 0.000 title claims abstract description 23
- NVNLLIYOARQCIX-MSHCCFNRSA-N Nisin Chemical compound N1C(=O)[C@@H](CC(C)C)NC(=O)C(=C)NC(=O)[C@@H]([C@H](C)CC)NC(=O)[C@@H](NC(=O)C(=C/C)/NC(=O)[C@H](N)[C@H](C)CC)CSC[C@@H]1C(=O)N[C@@H]1C(=O)N2CCC[C@@H]2C(=O)NCC(=O)N[C@@H](C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(NCC(=O)N[C@H](C)C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCSC)C(=O)NCC(=O)N[C@H](CS[C@@H]2C)C(=O)N[C@H](CC(N)=O)C(=O)N[C@H](CCSC)C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(N[C@H](C)C(=O)N[C@@H]3C(=O)N[C@@H](C(N[C@H](CC=4NC=NC=4)C(=O)N[C@H](CS[C@@H]3C)C(=O)N[C@H](CO)C(=O)N[C@H]([C@H](C)CC)C(=O)N[C@H](CC=3NC=NC=3)C(=O)N[C@H](C(C)C)C(=O)NC(=C)C(=O)N[C@H](CCCCN)C(O)=O)=O)CS[C@@H]2C)=O)=O)CS[C@@H]1C NVNLLIYOARQCIX-MSHCCFNRSA-N 0.000 title claims abstract description 23
- 239000004309 nisin Substances 0.000 title claims abstract description 23
- 235000010297 nisin Nutrition 0.000 title claims abstract description 23
- 235000013305 food Nutrition 0.000 title claims abstract description 14
- 238000000034 method Methods 0.000 title claims abstract description 12
- 238000004519 manufacturing process Methods 0.000 title abstract description 16
- 238000000855 fermentation Methods 0.000 claims abstract description 33
- 230000004151 fermentation Effects 0.000 claims abstract description 33
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229930006000 Sucrose Natural products 0.000 claims abstract description 12
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 12
- 239000005720 sucrose Substances 0.000 claims abstract description 12
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 11
- 240000008042 Zea mays Species 0.000 claims abstract description 10
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 10
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 10
- 235000005822 corn Nutrition 0.000 claims abstract description 10
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 9
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 9
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims abstract description 9
- 239000004310 lactic acid Substances 0.000 claims abstract description 8
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims abstract description 7
- 239000001888 Peptone Substances 0.000 claims abstract description 6
- 108010080698 Peptones Proteins 0.000 claims abstract description 6
- 235000019319 peptone Nutrition 0.000 claims abstract description 6
- 108010073771 Soybean Proteins Proteins 0.000 claims abstract description 4
- 235000019710 soybean protein Nutrition 0.000 claims abstract description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 16
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- 244000057717 Streptococcus lactis Species 0.000 claims description 14
- 235000014897 Streptococcus lactis Nutrition 0.000 claims description 14
- 239000002609 medium Substances 0.000 claims description 8
- 239000011780 sodium chloride Substances 0.000 claims description 8
- 230000001580 bacterial effect Effects 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 238000011218 seed culture Methods 0.000 claims description 6
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 4
- 229920000053 polysorbate 80 Polymers 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000005273 aeration Methods 0.000 claims description 2
- 230000003068 static effect Effects 0.000 claims description 2
- 239000002994 raw material Substances 0.000 abstract description 5
- 239000002440 industrial waste Substances 0.000 abstract description 4
- 239000002699 waste material Substances 0.000 abstract description 4
- 238000013459 approach Methods 0.000 abstract description 2
- 238000012545 processing Methods 0.000 abstract description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 235000011121 sodium hydroxide Nutrition 0.000 description 4
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
- 239000005864 Sulphur Substances 0.000 description 2
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 239000000811 xylitol Substances 0.000 description 2
- 229960002675 xylitol Drugs 0.000 description 2
- 235000010447 xylitol Nutrition 0.000 description 2
- WTLKTXIHIHFSGU-UHFFFAOYSA-N 2-nitrosoguanidine Chemical compound NC(N)=NN=O WTLKTXIHIHFSGU-UHFFFAOYSA-N 0.000 description 1
- CYDQOEWLBCCFJZ-UHFFFAOYSA-N 4-(4-fluorophenyl)oxane-4-carboxylic acid Chemical compound C=1C=C(F)C=CC=1C1(C(=O)O)CCOCC1 CYDQOEWLBCCFJZ-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- 241001478240 Coccus Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- ZGBSOTLWHZQNLH-UHFFFAOYSA-N [Mg].S(O)(O)(=O)=O Chemical compound [Mg].S(O)(O)(=O)=O ZGBSOTLWHZQNLH-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical compound CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000020265 peanut milk Nutrition 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 229930010796 primary metabolite Natural products 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 108010009004 proteose-peptone Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000001540 sodium lactate Substances 0.000 description 1
- 235000011088 sodium lactate Nutrition 0.000 description 1
- 229940005581 sodium lactate Drugs 0.000 description 1
- 235000010344 sodium nitrate Nutrition 0.000 description 1
- 239000004317 sodium nitrate Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Using the method for Wastage of food industry production nisin, it is characterized in that the bean dregs after being extracted using corn steep liquor, Klinit dregs and soybean protein make basal fermentation liquid, bound fraction sucrose and yeast pass through fermenting lactic acid streptostacin.The present invention utilizes numerous food industrial waste fermenting lactic acid streptostacin; instead of or part replace the raw material such as sucrose, dusty yeast, peptone; while production cost is greatly lowered; turn waste into wealth; largely decrease or even eliminate cost caused by waste harmless treatment; reach the purpose of comprehensive utilization of resources; simultaneously a kind of Environment-protection Approach is provided for the processing of these industrial wastes; the present invention realizes scrap loop use; environment is protected, also achieves being greatly lowered for production cost.
Description
Technical field
The present invention relates to technical field of microbial fermentation, and in particular to one kind utilizes Wastage of food industry production nisin
The method of rhzomorph.
Background technology
Nisin（group N inhibitory substance）, also known as nisin（Peptide）, streptococcus lactis peptide,
It is by streptococcus lactis（Streptococcus lactis ）Or Lactococcus lactis（Lactococcus lactis ）Part bacterium
Small molecule polypeptide compounds caused by strain fermentation.It can effectively suppress the gram-positive bacterium for causing food spoilage
And its growth of spore, extend Food Shelf-life.Meanwhile Nisin is easily dropped by some protease in human body alimentary canal
Solution, will not accumulate in human body, be universally acknowledged safe natural antiseptic agent.
Nisin production is that the growth with bacterial strain is closely related, and there are some researches show Nisin synthesis occurs in bacterium
Strain enters exponential phase, and when bacterial strain enters stationary phase, Nisin synthesis also stopped, and shows as primary metabolite dynamics spy
Sign.Nutrition of the lactic acid bacteria to fermentation medium is more fastidious, and the conventional carbon source of Nisin productions mainly has sucrose and glucose.
In actual production, sucrose is considered as best carbon source.Nitrogen source is an important factor for influenceing Nisin fermentation level, generally
Think soy peptone, dusty yeast, fish meal, cotton seed meal, tryptone, the preferable nitrogen source that casein peptone is production Nisin.But
Above-mentioned raw materials are expensive, certainly will increase production cost, select cheap carbon and nitrogen sources necessary.
Klinit dregs are the discarded objects for producing xylitol, and raw material is corncob, raw material sugar content 54%, extracted xylitol
Sugar content still has 20% or so afterwards, and main component is glucose and fructose, is not yet used for the carbon source material of fermentation industry at present, and one
Directly go out of use for boiler oil.
Corn steep liquor i.e. corn soaking waste water, it is to produce caused discarded object during starch sugar, it is beautiful in corn steep liquor
Containing abundant soluble protein, auxin and some precursor substances in Rice ＆ peanut milk, and containing saccharomycete, lactic acid bacteria etc., for breast
Acid streptococci strain growth provides abundant energy, and and can substantially reduces the usage amount of yeast in production process, and extracts lactic acid
Streptostacin can be made into sodium lactate with other residual nights later, the byproduct as nisin.
Bean dregs are to produce the residue during soybean protein, protein content 19%.Originally being used for low value feed always makes
With, if nitrogen source can be provided bean dregs addition zymotic fluid to streptococcus lactis, the production cost of nisin can be reduced,
Belong to comprehensive utilization of resources.
The content of the invention
In view of the deficienciess of the prior art, the invention provides one kind to utilize Wastage of food industry production streptococcus lactis
The method of element.
The technical solution adopted for the present invention to solve the technical problems is：
A kind of method that nisin is produced using Wastage of food industry, using corn steep liquor, Klinit dregs and soybean protein
Bean dregs after being extracted make basal fermentation liquid, and bound fraction sucrose and yeast pass through fermenting lactic acid streptostacin, specifically
Method and step is：.
Bacterial strain uses streptococcus lactis E-15；The composition of seed culture medium：In every liter of seed culture medium containing sucrose 5 ~
20g, containing 5 ~ 10g of dusty yeast, containing 5 ~ 10g of peptone, contain K2HPO41 ~ 10g, contain MgSO40.1 ~ 0.5 g, contains
NaCl 1~5g；Fermentation medium forms：80 ~ 120g/L of corn steep liquor；120 ~ 180g/L of Klinit dregs；30 ~ 80g/L of bean dregs,
K2HPO41 ~ 10 g/L, MgSO4The g/L of 0.1 ~ 0.5 g/L, NaCl 1 ~ 5, the g/L of Tween 80 0.1 ~ 1.0；Seed is in triangular flask
Middle culture, ferment and carried out in triangular flask or fermentation tank, seed and fermentation medium sterilising temp are 110 ~ 130 DEG C, are fermented
Journey rotating speed is 40 ~ 100rpm, using the fermentation that once feeds intake, controls pH5.0 ~ 7.0 with 20-30% NaOH, fermentation period is
30 hours.
Above-mentioned condition of culture be 28 DEG C ~ 37 DEG C at static gas wave refrigerator or 50 ~ 120rpm shaking table cultures, fermentation tank culture condition
Cultivated to close under air inlet or a small amount of aeration condition.
Above-mentioned streptococcus lactis E-15 is using streptococcus lactis ATCC11454 as starting strain, through ultraviolet, microwave, Asia
Sodium nitrate and nitrosoguanidine multiple mutated, and using Nisin as resistance screening agent, with reference to double-deck agar diffusion method,
One plant of Nisin superior strain finally is selected, is named as streptococcus lactis E-15.
The present invention utilize numerous food industrial waste fermenting lactic acid streptostacin, instead of or part instead of sucrose,
The raw materials such as dusty yeast, peptone, while production cost is greatly lowered, turn waste into wealth, largely decrease or even eliminate
Cost caused by waste harmless treatment, reach the purpose of comprehensive utilization of resources, while provided for the processing of these industrial wastes
A kind of Environment-protection Approach, the present invention realize scrap loop use, that is, protect environment, also achieve production cost significantly
Reduce.
Embodiment
Example 1
A kind of method that nisin is produced using Wastage of food industry, bacterial strain use streptococcus lactis E-15；Seed is trained
Support the composition of base：
Seed culture medium (per L)：Dusty yeast 3g, sodium chloride 1g, sucrose 5g, peptone 3g, dipotassium hydrogen phosphate 6g, sulphur
Sour magnesium 0.1g/L；With triangular flask culture seed, liquid amount is 100 mL/300mL.The shaking table under the conditions of 35 DEG C, 70rpm
Cultivate 10h；
Fermentation medium (per L)：Corn steep liquor 30g, bean dregs 10g, Klinit dregs 10g, sodium chloride 1g, dipotassium hydrogen phosphate
5g, magnesium sulfate 0.1g, Tween-80 1g；Batch fermentation, inoculum concentration 10%, fermentation process are carried out in 10L fermentation tanks
In pass through stream plus 20% liquid caustic soda, control pH6.5 or so, close ventilation culture speed of agitator 50rpm, 35 DEG C of temperature, seed
It is 121 DEG C with fermentation medium sterilising temp, using the fermentation that once feeds intake, pH6.5, fermentation week is controlled with 20% NaOH
Phase is 30 hours, final nisin potency 4378IU/mL.
Example 2
A kind of method that nisin is produced using Wastage of food industry, bacterial strain use streptococcus lactis E-15；Seed is trained
Support the composition of base：
Seed culture medium (per L)：Dusty yeast 3g, sodium chloride 1g, sucrose 5g, peptone 3g, dipotassium hydrogen phosphate 6g, sulphur
Sour magnesium 0.1g/L；With triangular flask culture seed, liquid amount is 100 mL/300mL.The shaking table under the conditions of 35 DEG C, 70rpm
Cultivate 10h；
Fermentation medium (per L)；Corn steep liquor 30g, bean dregs 10g, sucrose 10g, sodium chloride 1g, dipotassium hydrogen phosphate 5g, sulfuric acid
Magnesium 0.1g, Tween-80 1g；Carry out batch fermentation in 10L fermentation tanks, inoculum concentration 10%, pass through in fermentation process
Stream plus 20% liquid caustic soda control pH6.5 or so, close ventilation culture, speed of agitator 50rpm, 35 DEG C of temperature, final lactic acid chain
Coccus element potency 4832IU/mL.
Claims (2)
- A kind of 1. method that nisin is produced using Wastage of food industry, it is characterized in that using corn steep liquor, Klinit dregs Bean dregs after being extracted with soybean protein make basal fermentation liquid, and bound fraction sucrose and yeast pass through fermenting lactic acid hammer Rhzomorph, specific method step are：Bacterial strain uses streptococcus lactis E-15；The composition of seed culture medium：Contain 5 ~ 20g of sucrose in every liter of seed culture medium, contain There is 5 ~ 10g of dusty yeast, containing 5 ~ 10g of peptone, contain K2HPO41 ~ 10g, contain MgSO40.1 ~ 0.5 g, containing NaCl 1 ~ 5g；Fermentation medium forms：80 ~ 120g/L of corn steep liquor；120 ~ 180g/L of Klinit dregs；Bean dregs 30 ~ 80g/L, K2HPO4 1~10 G/L, MgSO4The g/L of 0.1 ~ 0.5 g/L, NaCl 1 ~ 5, the g/L of Tween 80 0.1 ~ 1.0；Seed is cultivated in triangular flask, fermentation Being carried out in triangular flask or fermentation tank, seed and fermentation medium sterilising temp are 110 ~ 130 DEG C, fermentation process rotating speed is 40 ~ 100rpm, using the fermentation that once feeds intake, pH5.0 ~ 7.0 are controlled with 20-30% NaOH, fermentation period is 30 hours.
- 2. the method according to claim 1 that nisin is produced using Wastage of food industry, it is characterized in that culture Condition is static gas wave refrigerator or 50 ~ 120rpm shaking table cultures at 28 DEG C ~ 37 DEG C, and fermentation tank culture condition is closing air inlet or few Cultivated under amount aeration condition.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110420972A (en) * | 2019-08-06 | 2019-11-08 | 德州正朔环保有限公司 | Solid waste biodegrade ferment |
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| CN1944658A (en) * | 2006-06-09 | 2007-04-11 | 山东大学 | Method for producing cellulose alcohol using corncob processing leftover by fermenting |
| CN101906453A (en) * | 2010-08-16 | 2010-12-08 | 环宇格林粮食开发有限公司 | Fermentation process for preparing nisin |
| CN101948893A (en) * | 2010-09-27 | 2011-01-19 | 郑州奇泓生物科技有限公司 | Method for producing nisin by continuous fermentation |
| CN102816817A (en) * | 2012-09-07 | 2012-12-12 | 吉林中粮生化科技有限公司 | Fermentation method of corn soaking water for producing nisin |
| CN103805660A (en) * | 2012-11-09 | 2014-05-21 | 山东福瑞达生物科技有限公司 | Method for producing nisin by using cheap raw materials |
-
2017
- 2017-06-08 CN CN201710425658.0A patent/CN107400688A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0581024A1 (en) * | 1992-06-26 | 1994-02-02 | New Japan Chemical Co.,Ltd. | Polyester compounds and organic gelling agents comprising same |
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