CN101081055B - Method for preparing anaerobic fermentation soy bean residue - Google Patents
Method for preparing anaerobic fermentation soy bean residue Download PDFInfo
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- CN101081055B CN101081055B CN2006100139698A CN200610013969A CN101081055B CN 101081055 B CN101081055 B CN 101081055B CN 2006100139698 A CN2006100139698 A CN 2006100139698A CN 200610013969 A CN200610013969 A CN 200610013969A CN 101081055 B CN101081055 B CN 101081055B
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Abstract
The anaerobically fermented soybean dregs features that it has the survival rate of anti-nutrilite, both heat stable and heat unstable, lower than 5 %, and the content of small peptide in molecular weight 19-48 KD in its protein higher than 90 %. It is prepared through crushing, sieving, spraying with fermenting liquid, sealed fermentation, stoving and packing. It is applied as protein feed material and can replace plasma protein, fish meal and other protein feed from animal, replace organic acid and antibiotic. It has the advantages of high anti-nutrilite deactivating rate, high stability and capacity of resulting in high raising economic benefit.
Description
(1) technical field:
The present invention relates to technical field of biological fermentation, especially a kind of anaerobic fermentation soya bean watse and production method thereof.
(2) background technology:
Livestock products security incidents such as the two mouthfuls of abusive tongue English pollution that takes place in succession both at home and abroad latter stage in last century, rabid ox disease, beta-stimulants poisoning have caused various circles of society's showing great attention to animal product and feed safety problem.At present, in order to improve foodsafety, utilize the plant protein resource of high-quality to substitute traditional animal protein feed in the world, to guarantee livestock products safety.
Dregs of beans is one of the most important vegetable protein of livestock and poultry source, and its crude protein content is up to 43-48%, and is made up of the amino acid than balance, and lysine content is up to 2.5-2.8%.But there is multiple ANFs in the dregs of beans, wherein mainly contain thermally labile ANFs such as trypsin inhibitor, soybean haemoglutinin, goitrin, urase and the antivitamin factor, and thermally-stabilised class ANFs such as phytic acid, oligosaccharide and glycinin, these factors have greatly influenced the feeding value and the application in children's livestock and poultry diet in age thereof of dregs of beans, and cause problems such as children's animal and bird intestines in age allergy and diarrhoea easily.In the dregs of beans production process, adopt heat treated mode that these ANFs are handled usually, then partly lose anti-oxidant action for the thermally labile ANFs, but still some is residual, however thermally-stabilised class ANFs still be present in the dregs of beans, can't eliminate.And, usually the dregs of beans production technology that adopts both at home and abroad need be handled the dregs of beans sterilization at present, the dry loss is bigger in Fa Jiao the process simultaneously, and in the process of aerobic fermentation, may have the reproductive processes of some harmful bacterium, the shared ratio of the peptide matters that produces in the sweat is on the low side in addition.Therefore, invent a kind of anaerobic fermentation soya bean watse and production method thereof, significant to the nutritive value that improves dregs of beans, also be the developing direction of dregs of beans product deep processing from now on.
(3) summary of the invention:
Goal of the invention of the present invention is to invent a kind of anaerobic fermentation soya bean watse and production method thereof, the particularly a kind of production method that can eliminate thermally-stabilised class ANFs such as soybean antigen in the dregs of beans, this method can solve ANFs content height in the existing market circulation dregs of beans protein raw material, causes problems such as children's animal and bird intestines in age allergy and diarrhoea easily.
Technical scheme of the present invention: a kind of anaerobic fermentation soya bean watse, the survival rate that it is characterized in that thermally labile ANFs and thermally-stabilised class ANFs in the anaerobic fermentation soya bean watse is less than 3-5%; Molecular weight is higher than 90% at the little peptide of 19-48KD in the anaerobic fermentation soya bean watse protein.
Above-mentioned said anaerobic fermentation soya bean watse uses and can substitute animal-based protein matter feedstuff in feed, can partly substitute organic acid, all or part of alternative antibiotic.
Above-mentioned said animal-based protein matter feedstuff can be plasma protein and fish meal.
A kind of production method of anaerobic fermentation soya bean watse is characterized in that it is finished by following steps:
(1) will desire fermented bean dregs and pulverize sieves,, after mixing in proportion, make fermentation seed fermented bacterium 1, fermented bacterium 2 and fermented bacterium 3;
(2) with fermentation seed and culture medium, the conditioning agent that promptly ferments, according to 1: the ratio of 1.5-2.5 is mixed;
(3) dregs of beans after will pulverizing and fermentation seed and fermentation conditioning agent mixture are according to 1000: the ratio of 2-6 is mixed, and will be mixed with the dregs of beans of fermented bacterium and water again and mix according to the ratio of 11-15: 8-5;
(4) dregs of beans that will inoculate behind the mixing directly is seated in the fermentation tank, and sealing prevents that moisture from distributing;
(5) be no less than 3 days at 30-50 ℃ condition bottom fermentation, the long-acting more fruit of fermentation time is good more;
(6) dregs of beans that the ferments livestock and poultry of can directly feeding form product 1;
(7) dregs of beans that ferments low temperature drying to moisture under 40-65 ℃ of condition is 12-15%, and product particle is remained between the 0.5-2mm, and the packing listing forms product 2.
The production method of above-mentioned said a kind of anaerobic fermentation soya bean watse, the fermentation process that whole employings under anaerobic utilize rumen microorganism and Bacillus acidi lactici to combine.
Above-mentioned said screen comprises 35-50 mesh sieve eye, and each mesh sieve eye diameter is 2-1.0mm.
Above-mentioned said fermented bacterium 1, fermented bacterium 2 and fermented bacterium 3 refer to that respectively the cud anaerobism is produced non-starch polysaccharide enzyme fungi N.frontalis, cud produces protease anaerobic bacteria bacteroides ruminicola and swine alimentary canal anaerobic bacteria Bacillus acidi lactici, and its proportioning is produced non-starch polysaccharide enzyme fungi N.frontalis for the cud anaerobism: cud produces protease anaerobic bacteria bacteroides ruminicola: swine alimentary canal anaerobic bacteria Bacillus acidi lactici is 1.2-2.3: 1.5-3: 1-2.1.
Above-mentioned said fermentation conditioning agent consist of glucose, dipotassium hydrogen phosphate K
2HPO
4, potassium dihydrogen phosphate KH
2PO
4, sodium chloride nacl, magnesium sulfate MgSO
4Mix with water, its ratio is 400-500: 4-6: 4-6: 5-6: 7-8: 12-16.
Organic acid content is not less than 5% in the above-mentioned said product 1, and the content of Bacillus acidi lactici is not less than hundred million/g of 50-80, and organic acid is not less than 10% in the product 2, and the content of Bacillus acidi lactici is not less than hundred million/g of 25-40.
Superiority of the present invention is: 1, need not dregs of beans is carried out sterilization treatment, whole process is an anaerobic fermentation, and the dry loss is less than 3%; 2, in the dregs of beans inactivation ratio of the thermally-stabilised class ANFs of thermally labile class ANFs up to more than 95%; 3, macro-molecular protein in the dregs of beans can be decomposed into micromolecular peptide class, improve dregs of beans protein utilization and livestock and poultry production performance; 4, produce lot of organic acids and beneficial microbe in the process of fermented bean dregs, can further reduce the generation of children's livestock and poultry diarrhea in age, reduce the use of antibiotics, improve culture benefit; 5, anaerobic fermentation soya bean watse good stability, the success rate height when guaranteeing livestock products safety, can reduce aquaculture cost, improves the culturing economic benefit; 6, technological process is easy and simple to handle, and the yield rate height is invested for a short time, both has been suitable for large-scale production, also is suitable for small-scale production.
(4) Figure of description:
Fig. 1 is the flow sheet of the production method of the related a kind of anaerobic fermentation soya bean watse of the present invention.
(5) specific embodiment:
Embodiment 1: a kind of anaerobic fermentation soya bean watse, and the survival rate that it is characterized in that thermally labile ANFs and thermally-stabilised class ANFs in the anaerobic fermentation soya bean watse is less than 3%; Molecular weight is higher than 90% at the little peptide of 19-48KD in the anaerobic fermentation soya bean watse protein.
Above-mentioned said anaerobic fermentation soya bean watse uses and can substitute animal-based protein matter feedstuff in feed, can partly substitute organic acid, all or part of alternative antibiotic.
Above-mentioned said animal-based protein matter feedstuff can be plasma protein and fish meal.
A kind of production method (see figure 1) of anaerobic fermentation soya bean watse is characterized in that it is finished by following steps:
(1) will desire fermented bean dregs and pulverize sieves,, after mixing in proportion, make fermentation seed fermented bacterium 1, fermented bacterium 2 and fermented bacterium 3;
(2) with fermentation seed and culture medium, the conditioning agent that promptly ferments mixes according to 1: 1.5 ratio;
(3) dregs of beans after will pulverizing mixes according to 1000: 5 ratio with fermentation conditioning agent mixture with fermentation seed, will be mixed with the dregs of beans of fermented bacterium and water again and mix according to 11: 8 ratio;
(4) dregs of beans that will inoculate behind the mixing directly is seated in the fermentation tank, and sealing prevents that moisture from distributing;
(5) 50 ℃ condition bottom fermentation 3 days;
(6) dregs of beans that the ferments livestock and poultry of can directly feeding form product 1;
(7) dregs of beans that ferments low temperature drying to moisture under 65 ℃ of conditions is 12%, and product particle is remained between the 0.5-2mm, and the packing listing forms product 2.
The production method of above-mentioned said a kind of anaerobic fermentation soya bean watse, the fermentation process that whole employings under anaerobic utilize rumen microorganism and Bacillus acidi lactici to combine.
Above-mentioned said screen comprises 35 mesh sieve eyes, and screen mesh diameter is 2mm.
Above-mentioned said fermented bacterium 1, fermented bacterium 2 and fermented bacterium 3 refer to that respectively the cud anaerobism is produced non-starch polysaccharide enzyme fungi N.frontalis, cud produces protease anaerobic bacteria bacteroides ruminicola and swine alimentary canal anaerobic bacteria Bacillus acidi lactici, and its proportioning is produced non-starch polysaccharide enzyme fungi N.frontalis for the cud anaerobism: cud produces protease anaerobic bacteria bacteroides ruminicola: swine alimentary canal anaerobic bacteria Bacillus acidi lactici is 2.3: 3: 1.2.
Above-mentioned said fermentation conditioning agent consist of glucose, dipotassium hydrogen phosphate K
2HPO
4, potassium dihydrogen phosphate KH
2PO
4, sodium chloride nacl, magnesium sulfate MgSO
4Mix with water, its ratio is 500: 6: 6: 6: 7: 1.2.
Organic acid content is not less than 5% in the above-mentioned said product 1, and the content of Bacillus acidi lactici is for being not less than 8,000,000,000/g, and organic acid is 10% in the product 2, and the content of Bacillus acidi lactici is 4,000,000,000/g.
Embodiment 2: a kind of anaerobic fermentation soya bean watse, and the survival rate that it is characterized in that thermally labile ANFs and thermally-stabilised class ANFs in the anaerobic fermentation soya bean watse is less than 5%; Molecular weight is higher than 90% at the little peptide of 19-48KD in the anaerobic fermentation soya bean watse protein.
Above-mentioned said anaerobic fermentation soya bean watse uses and can substitute animal-based protein matter feedstuff in feed, can partly substitute organic acid, all or part of alternative antibiotic.
Above-mentioned said animal-based protein matter feedstuff can be plasma protein and fish meal.
A kind of production method (see figure 1) of anaerobic fermentation soya bean watse is characterized in that it is finished by following steps:
(1) will desire fermented bean dregs and pulverize sieves,, after mixing in proportion, make fermentation seed fermented bacterium 1, fermented bacterium 2 and fermented bacterium 3;
(2) with fermentation seed and culture medium, the conditioning agent that promptly ferments mixes according to 1: 2.5 ratio;
(3) dregs of beans after will pulverizing mixes according to 1000: 2 ratio with fermentation conditioning agent mixture with fermentation seed, will be mixed with the dregs of beans of fermented bacterium and water again and mix according to 15: 5 ratio;
(4) dregs of beans that will inoculate behind the mixing directly is seated in the fermentation tank, and sealing prevents that moisture from distributing;
(5) 30 ℃ condition bottom fermentation 5 days, the long-acting more fruit of fermentation time was good more;
(6) dregs of beans that the ferments livestock and poultry of can directly feeding form product 1;
(7) dregs of beans that ferments low temperature drying to moisture under 65 ℃ of conditions is 15%, and product particle is remained between the 0.5-2mm, and the packing listing forms product 2.
The production method of above-mentioned said a kind of anaerobic fermentation soya bean watse, the fermentation process that whole employings under anaerobic utilize rumen microorganism and Bacillus acidi lactici to combine.
Above-mentioned said screen comprises 50 mesh sieve eyes, and screen mesh diameter is 1.0mm.
Above-mentioned said fermented bacterium 1, fermented bacterium 2 and fermented bacterium 3 refer to that respectively the cud anaerobism is produced non-starch polysaccharide enzyme fungi N.frontalis, cud produces protease anaerobic bacteria bacteroides ruminicola and swine alimentary canal anaerobic bacteria Bacillus acidi lactici, and its proportioning is produced non-starch polysaccharide enzyme fungi N.frontalis for the cud anaerobism: cud produces protease anaerobic bacteria bacteroides ruminicola: swine alimentary canal anaerobic bacteria Bacillus acidi lactici is 1.2: 1.5: 1.
Above-mentioned said fermentation conditioning agent consist of glucose, dipotassium hydrogen phosphate K
2HPO
4, potassium dihydrogen phosphate KH
2PO
4, sodium chloride nacl, magnesium sulfate MgSO
4Mix with water, its ratio is 400: 4: 4: 5: 7: 1.6;
Organic acid content is not less than 5% in the above-mentioned said product 1, and the content of Bacillus acidi lactici is 5,000,000,000/g, and organic acid is 10% in the product 2, and the content of Bacillus acidi lactici is 2,500,000,000/g.
Claims (2)
1. the production method of an anaerobic fermentation soya bean watse is characterized in that it is finished by following steps:
(1) will desire fermented bean dregs and pulverize sieves, fermented bacterium is produced non-starch polysaccharide enzyme fungi N.frontalis according to the cud anaerobism respectively: cud produces protease anaerobic bacteria bacteroides ruminicola: swine alimentary canal anaerobic bacteria Bacillus acidi lactici is after the ratio of 1.2-2.3: 1.5-3: 1-2.1 is mixed, to make fermentation seed;
(2) with fermentation seed and fermentation conditioning agent, according to 1: the ratio of 1.5-2.5 is mixed; Described fermentation conditioning agent consist of glucose, dipotassium hydrogen phosphate K
2HPO
4, potassium dihydrogen phosphate KH
2PO
4, sodium chloride nacl, magnesium sulfate MgSO
4Mix with water, its ratio is 400-500: 4-6: 4-6: 5-6: 7-8: 1.2-1.6;
(3) dregs of beans that will pulverize sieves and the mixture of fermentation seed and fermentation conditioning agent are according to 1000: the ratio of 2-6 is mixed, and will be mixed with the dregs of beans of fermented bacterium and water again and mix according to the ratio of 11-15: 8-5;
(4) dregs of beans that will inoculate behind the mixing directly is seated in the fermentation tank, and sealing prevents that moisture from distributing;
(5) be no less than 3 days at 30-50 ℃ condition bottom fermentation, the long-acting more fruit of fermentation time is good more;
(6) dregs of beans that ferments forms product 1, the livestock and poultry of directly feeding;
(7) dregs of beans that ferments low temperature drying to moisture under 40-65 ℃ of condition is 12-15%, and product particle is remained between the 0.5-2mm, and the packing listing forms product 2;
The thermally labile ANFs in the anaerobic fermentation soya bean watse and the survival rate of thermally-stabilised class ANFs are less than 5%; Molecular weight is higher than 90% at the little peptide of 19-48KD in the anaerobic fermentation soya bean watse protein; Anaerobic fermentation soya bean watse uses and substitute plasma protein and fish meal in feed, and part substitutes organic acid, all or part of alternative antibiotic.
2. according to the production method of a kind of anaerobic fermentation soya bean watse described in the claim 1, it is characterized in that organic acid content is not less than 5% in the described product 1, the content of Bacillus acidi lactici is not less than 5,000,000,000/g, and organic acid is not less than 10% in the product 2, and the content of Bacillus acidi lactici is not less than 2,500,000,000/g.
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| CN101965903B (en) * | 2010-08-17 | 2013-07-10 | 钱国英 | Preparation method of fermented vegetable protein |
| CN102178060B (en) * | 2011-05-13 | 2013-06-05 | 湖北邦之德牧业科技有限公司 | Biological polypeptide for replacing plasma protein in feed |
| CN102823717B (en) * | 2012-09-14 | 2014-02-19 | 浙江诚元生物技术有限公司 | Preparation method of high-bioactivity fermented soybean meal |
| CN103652323A (en) * | 2012-09-23 | 2014-03-26 | 周相超 | Technology for producing composite fermented feed raw material with non-drying fermentation method |
| CN102972854B (en) * | 2012-12-17 | 2015-06-03 | 青岛根源生物技术集团有限公司 | Production device and method for producing feed soybean meal by utilizing anaerobe fermentation |
| CN103385360B (en) * | 2013-08-14 | 2014-04-09 | 双胞胎（集团）股份有限公司 | Simple and efficient fermented soybean meal production technology and application thereof |
| CN103918886A (en) * | 2014-04-21 | 2014-07-16 | 广州东凌粮油股份有限公司 | Method for producing high-activity fermented soybean meal |
| CN104256200B (en) * | 2014-10-17 | 2017-09-15 | 黑龙江天予生物科技有限责任公司 | Support the preparation method of the fresh and alive bacterium peptide mixed feed of pig antibiotic-free |
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