CN105660098A - Method for reducing honeysuckle cadmium accumulation and increasing yield of honeysuckle by means of glomus intraradices - Google Patents
Method for reducing honeysuckle cadmium accumulation and increasing yield of honeysuckle by means of glomus intraradices Download PDFInfo
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- CN105660098A CN105660098A CN201610016838.9A CN201610016838A CN105660098A CN 105660098 A CN105660098 A CN 105660098A CN 201610016838 A CN201610016838 A CN 201610016838A CN 105660098 A CN105660098 A CN 105660098A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Abstract
The invention discloses a method for reducing honeysuckle cadmium accumulation and increasing the yield of honeysuckle by means of glomus intraradices. According to the method, glomus intraradices and honeysuckle are used for symbiotic cultivation. Specifically, after glomus intraradices is subjected to propagation cultivation by means of a host plant, a mixture containing host plant root sections, fungus hyphae, mycorrhiza fungal spores and cultivation soil is harvested, namely a glomus intraradices inoculant; then, the glomus intraradices inoculant is inoculated in a culture substrate for honeysuckle cultivation. The method can promote growth of honeysuckle and increase the yield. More importantly, cadmium can be prevented from transferring from the root to the overground portions, and the concentration of cadmium of the overground portions (stems, leaves and flowers) is reduced. A means is provided for safe honeysuckle production in soil polluted by cadmium, and the method plays an important role in honeysuckle production and application. The method is good in effect, free of secondary pollution and low in cost. A new thinking and a new direction are provided for development and utilization of glomus intraradices.
Description
Technical field
The invention belongs to heavy metal pollution recovery technique field. The Cd accumulation that Glomus intraradices reduces Flos Lonicerae the method improving its yield is utilized more particularly, to a kind of.
Background technology
Owing to Wastewater Irrigation in Farmland, solid waste are used and the mankind's activity such as mining and smelting, Cadmium Pollution in Soils is increasingly severe. Cadmium mobility in soil is big, toxicity is high, is one of inorganic pollution the most malicious in environment. Cadmium enters plant after being absorbed by plants, and can pass through food chain enrichment and enter human body, and agricultural production and human health in serious threat. For the arable land that large area mild or moderate pollutes, still lack economically viable technological means at present and thoroughly remove heavy metal-polluted soil. China has a large population and ploughs few particularly thorny, and being carried out in large area heavy metal pollution soil lies fallow can affect production estimation and national security is stable, and in China, this is unpractical. Content of beary metal is reduced in crops to ensure agricultural production security therefore, it is necessary to take measures.
Flos Lonicerae (Lonicerajaponica) is the perennial half evergreen liana of Caprifoliaceae Lonicera, is distributed widely in subtropical and tropical zones, and its aerial parts is medicinal effects, has significantly high medicinal and economic worth. It addition, Flos Lonicerae has, Biomass is big, growth is fast, life-span length, well developed root system, resistance, wide adaptability, to features such as soil requirement are not strict. But, recent research indicate that Flos Lonicerae accumulation cadmium very capable (Liu etc., 2009; Liu Zhouli etc., 2009; Liu etc., 2012; Jia etc., 2013; Jia Lian etc., 2013), production and the application of Flos Lonicerae are brought very bad impact by this. Therefore, the research reducing Flos Lonicerae Cd accumulation is to realize the important step that Flos Lonicerae is kept the safety in production on cadmium pollution soil. Lichen, black wood charcoal soil, plant fruit absorbent processed and Caulis et Folium Oryzae is adopted to consider to be worth doing as substrate culture Flos Lonicerae to (2013) such as words, these substrate both can provide abundant nutrition for plant growing, adsorbable fixing soil heavy metal again, reduce the biological effectiveness of heavy metal, heavy metal is stoped to pass in Flos Lonicerae body from contaminated soil, realize safety cultivation and production Flos Lonicerae in contaminated soil, but the method is not but suitable for large-area soil cultivation.
In prior art, utilize biological, physical chemistry or Agro-ecology measure can reduce cadmium accumulation in plant, be the important measures realizing carrying out safe agricultural production in cadmium pollution soil. Wherein, using soil conditioner is the effective means controlling Cadmium Pollution in Soils, its objective is to reduce the biological effectiveness of Cadmium in Soil, reaches original position and suppresses the purpose of Crop cadmium. The method cost is low, be prone to practice, is carry out one of effective way of keeping the safety in production on cadmium pollution soil. But, cadmium pollution soil modifying agent conventional at present, majority there is effect instability or to defects such as soil property, structure adversely affect, the reality directly affecting soil conditioner continues to apply. And utilizing mycorrhizal fungi to reduce plant Metal uptake amount is a kind of technological means effective, eco-friendly. After (1981) report Chinese photinia (Callunavulgaris) mycorhiza such as Bradley in 1981 reduce the plant absorption to excess copper and zinc, the research of mycorhiza Yu heavy metal pollution is created keen interest by people. Amir etc. (2013) also report that determine to grow significantly (p < 0.05) of Glomusetunicatum reduces the Ni concentration of its host plant Sorghumvulgare root and aerial parts and promoted growth.
At present, there is not yet and utilize mycorrhizal fungi to reduce the report of Cd accumulation in Flos Lonicerae.
Summary of the invention
The technical problem to be solved in the present invention is the deficiency overcoming and reducing Flos Lonicerae Cd accumulation method in prior art, it is provided that one utilizes the Cd accumulation that Glomus intraradices (Rhizophagusintraradices) reduces Flos Lonicerae the method improving Flos Lonicerae yield. Utilizing Glomus intraradices and Flos Lonicerae symbiosis, be possible not only to improve the mineral nutrient status of Flos Lonicerae, especially the nutrition of P element, thus promoting that Flos Lonicerae grows, increasing Biomass especially the upperground part biomass; The more important thing is, it is possible to stop cadmium to shift to aerial parts from root, reduce the cadmium concentration of aerial parts (stem, Ye Hehua), also reduce the Cd murder by poisoning to Flos Lonicerae.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
First, the invention discloses Glomus intraradices application in the Cd accumulation reducing Flos Lonicerae and/or raising Flos Lonicerae yield, this application all should within protection scope of the present invention.
On this basis, the invention provides and a kind of utilize Glomus intraradices to reduce Flos Lonicerae Cd accumulation the method that improves its yield, be utilize Glomus intraradices and Flos Lonicerae symbiotic cultivation.
Preferably, described Glomus intraradices is Glomus intraradices microbial inoculum.
Described Glomus intraradices microbial inoculum can be common Glomus intraradices microbial inoculum.
As one preferred embodiment, described Glomus intraradices microbial inoculum is the mixture including Glomus intraradices host plant root segment, mycelia, mycorrhizal fungal spore and/or cultivation matrix.
More preferably, above-mentioned Glomus intraradices is utilized to reduce Flos Lonicerae Cd accumulation and improve the method for its yield specifically: after Glomus intraradices being carried out expanding propagation cultivation first with host plant, the results mixture containing host plant root segment, fungal mycelia, mycorrhizal fungal spore and cultivating soil, is Glomus intraradices microbial inoculum; Again Glomus intraradices microbial inoculum is inoculated in cultivation matrix, carries out the cultivation of Flos Lonicerae.
Wherein it is preferred to, described host plant is the host plant of Glomus mosseae.
It is highly preferred that the host plant of described Glomus mosseae is Semen Maydis, Herba Astragali Melilotoidis (Herba Astragali Sinici), Sorghum vulgare Pers., Herba Trifolii Pratentis or arabian cron etc.
Preferably, described Glomus intraradices is Glomus intraradices BGCAH01.
Preferably, the described substrate utilizing host plant Glomus intraradices to carry out expanding propagation cultivation used is sandy soil.
It is highly preferred that the sand of the weight ratio 1:1 that described sandy soil are sterilizing and earth mixtures.
Furthermore it is preferred that when above-mentioned expanding propagation is cultivated, the inoculum concentration of Glomus intraradices is 0.1～20w/w%.
It is highly preferred that when expanding propagation is cultivated, the inoculum concentration of described Glomus intraradices is 1～5w/w%.
Most preferably, when expanding propagation is cultivated, the inoculum concentration of described Glomus intraradices is 3～4w/w%.
Preferably, during Flos Lonicerae cultivation, the inoculum concentration of described Glomus intraradices microbial inoculum is 0.1～20w/w%.
It is highly preferred that during Flos Lonicerae cultivation, the inoculum concentration of described Glomus intraradices microbial inoculum is 1～5w/w%.
Most preferably, during Flos Lonicerae cultivation, the inoculum concentration of described Glomus intraradices microbial inoculum is 3～4w/w%.
As one preferably can embodiment, the concrete preparation method of above-mentioned Glomus intraradices microbial inoculum is as follows:
Inoculum concentration according to 0.1～20w/w%, by evenly laid out for Glomus intraradices Inoculant in sandy soil substrate, cover lid layer sterilizing sandy soil substrate, water, the seed of sowing Glomus intraradices host, cover lid layer sterilizing sandy soil substrate again, cultivate 3～6 months, after removing above-ground plant parts stem and leaf, it is placed in the metastable space of temperature and humidity to dry 10～15 days, then gathers in the crops all cultures, including plant root segment, mycelia, mycorrhizal fungal spore and cultivation matrix, mince mixing, namely obtain Glomus intraradices microbial inoculum. Wherein it is preferred to, described inoculum concentration is 1～5w/w%. It is highly preferred that described inoculum concentration is 3～4w/w%. Preferably, described sandy soil substrate is sand and the earth mixtures of the weight ratio 1:1 of sterilizing.
It addition, the operation after all cultures of above-mentioned results is accomplished aseptic as far as possible, concretely: operation desktop is first with ethanol (such as 70% ethanol) cleaning disinfection; Desktop covers the paper that some layers (such as 3 layers) are clean, the culture of results is poured on paper, minces with the hatchet of flame disinfection; Roll the culture minced with two pieces of paper, one end of paper web is inserted in the pre-prepd clean plastic bag that can seal; After all of culture is all transferred in bag, takes out paper gently, seal up bag, whenever all do not allow hands touch a bit potted plant culture, then one identical plastic bag of surface cover outside, it is then placed within preservation in 4 DEG C of refrigerators.
More specifically, the preparation method of described Glomus intraradices microbial inoculum is as follows:
The sandy soil of dress sterilizing are (according to weight ratio, husky: soil=1:1) culture medium is to the 4/5 of plastic tub; According to the inoculum concentration of 4w/w%, by evenly laid out for Glomus intraradices Inoculant a thin layer in substrate. Cover sterilization matrix 2cm again, water, 10～15/basin of seeding corn seed. Cover 0.5cm sterilization matrix, then move to hot-house culture;
Gather in the crops after potted plant 5 months; During results, first cut off plant strain aerial parts stem and leaf, basin is placed in the metastable room of temperature humidity and dries two weeks, then all cultures (including root system of plant, mycelia, spore and substrate) in results basin;
Operation desktop is first sterilized by 70% alcohol wipe; Desktop covers paper 3 layers clean, culture in basin is poured on paper, minces with the hatchet of flame disinfection; Roll the culture minced with two pieces of paper, one end of paper web is inserted in the pre-prepd clean plastic bag that can seal;After all of culture is all transferred in bag, takes out paper gently, seal up bag, whenever all do not allow hands touch a bit potted plant culture, then one identical plastic bag of surface cover outside, it is then placed within preservation in 4 DEG C of refrigerators.
It addition, a kind of soil conditioner being applicable to plant on cadmium pollution soil Flos Lonicerae including Glomus intraradices also should within protection scope of the present invention. Described Glomus intraradices can be above-mentioned Glomus intraradices microbial inoculum.
Preferably, in described soil conditioner, the content of Glomus intraradices microbial inoculum is 0.1～20w/w%, and the consumption that makes of described soil conditioner is 3.4～668 kgs/acre.
It is highly preferred that in described soil conditioner, the content of Glomus intraradices microbial inoculum is 1～5w/w%, the consumption that makes of described soil conditioner is 34～167 kgs/acre.
Most preferably, in described soil conditioner, the content of Glomus intraradices microbial inoculum is 3～4w/w%, and the consumption that makes of described soil conditioner is 100 kgs/acre.
It addition, also it is emphasized that based on the Glomus intraradices disclosed in this invention application in reducing the Cd accumulation of Flos Lonicerae and/or improving Flos Lonicerae yield, other schemes derived, replace, being combined into, all should within protection scope of the present invention.
The present invention is with Flos Lonicerae for experimental plant, with Glomus intraradices for Inoculant, in different Cd concentration (0,10 and 20mgCdKg-1) soil in, the potentiality of Flos Lonicerae accumulation cadmium are reduced, it is shown that inoculation Glomus intraradices (Ri) improves Flos Lonicerae absorbs the ability of P by potted plant experiment research inoculation Glomus intraradices, having promoted Flos Lonicerae growth, Biomass increases considerably; And, inoculation Ri can stop cadmium to shift to aerial parts from Radix Flos Lonicerae, reduces aerial parts (stem, Ye Hehua) cadmium concentration, it is achieved that produce the purpose of Flos Lonicerae on cadmium pollution soil.
The method have the advantages that
The present invention utilizes Glomus intraradices and Flos Lonicerae symbiosis, is possible not only to improve the mineral nutrient status of Flos Lonicerae, especially the nutrition of P element, thus promoting that Flos Lonicerae grows, increasing Biomass especially the upperground part biomass, improving the yield of Flos Lonicerae; The more important thing is, it is possible to stoping cadmium to shift to aerial parts from root, reduce the cadmium concentration of aerial parts (stem, Ye Hehua), production and application to Flos Lonicerae have great importance.
It addition, The invention also achieves a kind of new opplication of Glomus intraradices, the development and application for Glomus intraradices provides a new thinking and direction.
The method of the present invention is with traditional chemistry compared with agricultural measures, and the measure of inoculation Glomus intraradices has the features such as effective, non-secondary pollution, operating cost are low.
Detailed description of the invention
Further illustrate the present invention below in conjunction with specific embodiment, but the present invention is not limited in any form by embodiment. Unless stated otherwise, the present invention adopts reagent, method and apparatus are the art conventional reagent, method and apparatus.
Unless stated otherwise, following example agents useful for same and material are commercial.
Embodiment 1
1, the preparation of the cultivation of strains tested expanding propagation and microbial inoculum
Glomus intraradices (Rhizophagusintraradices is called for short Ri) BGCAH01 is provided by Inst. of Plant Nutrition &. Resource, Beijing City Academy of Agricultural &. Fo.
After utilizing Semen Maydis (Zeamays) to carry out potted plant expanding propagation as host plant, using the mixture containing host plant root segment, fungal mycelia, mycorrhizal fungal spore and potted plant soil as inoculating microbial inoculum. Detailed process is as follows:
The sandy soil of dress sterilizing are (according to weight ratio, husky: soil=1:1) culture medium is to the 4/5 of plastic tub.Inoculum concentration (w:w) according to 4% is by evenly laid out for Glomus intraradices Inoculant a thin layer in substrate. Cover sterilization matrix 2cm again, water, 10～15/basin of seeding corn seed. Cover 0.5cm sterilization matrix, then move to hot-house culture.
Gather in the crops after potted plant 5 months. During results, first cut off plant strain aerial parts stem and leaf, basin is placed in the metastable room of temperature humidity and dries two weeks, then all cultures (including root system of plant, mycelia, spore and substrate) in results basin.
Operation desktop is first sterilized by 70% alcohol wipe. Desktop covers paper 3 layers clean, culture in basin is poured on paper, minces with the hatchet of flame disinfection. Roll the culture minced with two pieces of paper, one end of paper web is inserted in the pre-prepd clean plastic bag that can seal. After all of culture is all transferred in bag, takes out paper gently, seal up bag, whenever all do not allow hands touch a bit potted plant culture, then one identical plastic bag of surface cover outside, it is then placed within preservation in 4 DEG C of refrigerators.
2, test plant
Test plant is Flos Lonicerae.
In April, 2014, gather biennial Flos Lonicerae branch as cutting. Choose about length 30cm, branch that thickness is consistent, leaf is not stayed in branch upper end, and lower end is whittled into smooth ramp. Sand culture cuttage is cultivated 6 weeks, until when trophophyll grows 2～3, selecting the seedling that growing way is consistent, through 0.3%KMnO4Its root is sterilized after 5s by solution, rinses successively for several times with tap water and distilled water.
3, for examination soil
Soil picks up from South China Normal University's biology garden.
The basic physical and chemical of soil is as follows: pH6.65, organic substance 1.45%, available phosphorus 52mg/kg, total Cd0.14mg/kg, DTPA-Cd0.063mg/kg.
Soil is ground after natural air drying, after crossing 1mm sieve, 121 DEG C of high temperature and high pressure steam sterilizing 2h, it is separately added into the CdCl of variable concentrations2Cd concentration is made to reach 10mg/kg and 20mg/kg.
In order to allow Cd in soil be evenly distributed and stable, add after deionized water infiltrates one week air-dry two weeks, circulate twice.
4, the application of Glomus intraradices inoculation Flos Lonicerae
(1) experimental design: test is divided into 3 groups: three kinds of heavy metal-polluted soil Cd concentration to be respectively as follows: 0mg/kg, 10mg/kg, 20mg/kg. Often organize all design inoculation and not inoculating two kinds process.
(2) test basin is the plastic flowerpot of capacity 3kg soil, by the inoculum concentration of 3w/w% by dress basin after Glomus intraradices microbial inoculum and soil Homogeneous phase mixing, do not inoculate the inactivation microbial inoculum of the Glomus intraradices after twice sterilizing accessing equivalent in comparison, and microbial inoculum filters the bacteria suspension after Glomus intraradices (supplementing the loss of antibacterial in inactivation microbial inoculum) through filter paper (whatmanNo.1, aperture: 11 μm). The Flos Lonicerae seedling that every pot transplanting 3 strain upgrowth situation is good and upgrowth situation is consistent, each process repeats 5 times.
(3) potted plant being placed in greenhouse at random being cultivated, illumination 14h, temperature is 22 DEG C～28 DEG C, within every two days, waters a sterilized water. Plant is gathered in the crops after cultivating 14 months.
5, experimental index measures
(1) mensuration of Infection Rate
With distilled water, root is cleaned, be cut into the segment of about 1cm length, put in the KOH solution of 15%, 90 DEG C of water bath with thermostatic control 30min, root sample is rinsed gently with distilled water; With the H of 10%2O2Bleaching 30min, with distilled water flushing; Again root is immersed in the HCl solution of 1% and stand 3min, after washing away HCl solution with distilled water, add the Trypanblue dye liquor of 0.05%, dye under 90 DEG C of water-baths 30min, after dyeing, wash away the loose colour of root with acid glycerol (lactic acid: glycerol 1:1), 50 root segments of random picking infect situation in basis of microscopic observation.Method with reference to (1980) such as Giovannetti calculates Glomus intraradices infection strength.
(2) mensuration of honeysuckle biological amount
After plant harvest, rinse well with deionized water, separately Plant aboveground (stem, Ye Hehua) and under ground portion, be placed in 80 DEG C of baking ovens 72h matter constant weight, claim dry weight.
(3) mensuration of Flos Lonicerae Cd content
Total Cd content adopts HNO3-HClO4-HF clears up, with Cd content in TAS-986 flame type atomic absorption measuring solution.
(4) soil available cadmium assay
With reference to Lindsay and Norvell (1978), extract-AAS method with DTPA and measure effective Cd in soil.
(5) plant phosphorus detection
By plant root, stem and leaf each several part clear water wash clean, be then placed in baking oven 75 DEG C dry 48h, until constant weight. The root, stem and leaf each several part Sulfuric-acid-hydrogen-peroxide weighing drying respectively digests, and after cooling dilution, P content adopts molybdenum-antimony anti-spectrophotometric method to measure.
(6) plant anti-oxidation index determining
Take the blade 0.5g of plant, be ground to rapidly Powdered in liquid nitrogen, add the phosphate buffer (TritonX-100 containing PVPP and 0.3% that mass percent concentration is 4%) of the 50mmol/LpH7.8 of 6ml pre-cooling. And with the centrifugal 20min of 10000r/min, supernatant is enzyme liquid, and 4 DEG C save backup.
SOD activity measures with reference to the method for Beyer and Fridovich (1987). It is not added with the irradiation pipe of NBT for comparison with not enzyme-added liquid simultaneously, adds the irradiation pipe of NBT for maximum photo-reduction pipe with not enzyme-added liquid simultaneously, with enzyme amount needed for restraining NBT photo-reduction 50% for a unit of enzyme activity (U).
APX activity measures with reference to the method for (1981) such as Nakano.
GR activity measures with reference to the method for Carlberg and Mannervik (1985).
CAT activity measures with reference to the method for Aebi (1984).
MDA content measures with reference to the method for Heath and Packer (1968).
6, experimental result
(1) the inoculation Ri impact on honeysuckle biological amount and P content, result is as shown in table 1. Result shows:
1) not inoculating in Ri process, Flos Lonicerae does not have Ri to infect. The infection rate of Ri is not affected by soil Cd concentration.
2) inoculation Ri has been obviously promoted the growth of Flos Lonicerae, substantially increases with underground biomass on the ground, maximum amplification respectively 268% and 244%.
3) inoculation Ri significantly improves Flos Lonicerae P content.
The Ri impact (mean+SD, n=5) on honeysuckle biological amount and P content inoculated by table 1
Significant difference (p < 0.05) between representing inoculation and not inoculating.
(2) the inoculation Ri impact on Flos Lonicerae Cd concentration and soil DTPA-Cd, result is as shown in table 2.
Result shows:
1) inoculation Ri significantly reduces the Cd concentration of stem of Flos Lonicerae, Ye Hehua, the range of decrease respectively 66%, 65%, 72% (Cd10) and 60%, 68%, 76% (Cd20);
2) inoculation Ri significantly improves the Cd concentration of Radix Flos Lonicerae, amplification respectively 62% (Cd10) and 28% (Cd20);
3) rhizosphere soil DTPA-Cd concentration is had no significant effect by inoculation Ri.
The Ri impact (mean+SD, n=5) on Flos Lonicerae Cd concentration and soil DTPA-Cd inoculated by table 2
* significant difference (p < 0.05) between representing inoculation and not inoculating.
(3) the inoculation Ri impact on Flos Lonicerae antioxidase and MDA, result is as shown in table 3.
Result shows:
1) inoculation Ri significantly improves Flos Lonicerae blade CAT, GR and APX activity, improves oxidation resistance, thus alleviating the cadmium toxic action to Flos Lonicerae.
2) Flos Lonicerae sod activity is had no significant effect by inoculation Ri.
3) inoculation Ri significantly reduces Flos Lonicerae blade MDA content.
The table 3Rhizophagusintraradices impact (mean+SD, n=5) on Flos Lonicerae antioxidase and MDA
* significant difference (p < 0.05) between representing inoculation and not inoculating. SOD, CAT, GR, APX and MDA respectively superoxide dismutase, catalase, glutathion reductase, ascorbate peroxidase enzyme and malonaldehyde.
In sum, can draw the following conclusions and analyze:
(1) in Glomus intraradices-Flos Lonicerae homobium, extend to the outer mycelia of the root in soil in a large number and expand the absorption area of root system, improve the mineral nutrient status of Flos Lonicerae, especially the nutrition of P element, thus promoting that Flos Lonicerae grows, increase Biomass especially the upperground part biomass.
(2) Glomus intraradices improves the antioxidation of the antioxidative defense enzyme such as Flos Lonicerae catalase (CAT), ascorbate peroxidase enzyme (APX) and glutathion reductase (GR); reduce MDA content, thus reducing the Cd murder by poisoning to Flos Lonicerae.
(3) Glomus intraradices improves Radix Flos Lonicerae cadmium concentration, reduces aerial parts (stem, Ye Hehua) cadmium concentration, stops cadmium to shift to aerial parts from root.
Claims (10)
1. Glomus intraradices application in the Cd accumulation reducing Flos Lonicerae and/or raising Flos Lonicerae yield.
2. one kind utilizes Glomus intraradices to reduce Flos Lonicerae Cd accumulation the method improving its yield, it is characterised in that be utilize Glomus intraradices and Flos Lonicerae symbiotic cultivation.
3. method according to claim 2, it is characterised in that described Glomus intraradices is Glomus intraradices microbial inoculum, described Glomus intraradices microbial inoculum is the mixture including Glomus intraradices host plant root segment, mycelia, mycorrhizal fungal spore and/or cultivation matrix.
4. method according to claim 3, it is characterized in that, it is after Glomus intraradices being carried out expanding propagation cultivation first with host plant, the results mixture containing host plant root segment, fungal mycelia, mycorrhizal fungal spore and cultivating soil, it is Glomus intraradices microbial inoculum; Again Glomus intraradices microbial inoculum is inoculated in cultivation matrix, carries out the cultivation of Flos Lonicerae.
5. method according to claim 4, it is characterised in that described host plant is the host plant of Glomus mosseae.
6. method according to claim 4, it is characterised in that the described substrate utilizing host plant Glomus intraradices to carry out expanding propagation cultivation used is sandy soil.
7. method according to claim 4, it is characterised in that during described expanding propagation, the inoculum concentration of Glomus intraradices is 0.1～20w/w%.
8. method according to claim 4, it is characterised in that described in when carrying out the cultivation of Flos Lonicerae, the inoculum concentration of Glomus intraradices microbial inoculum is 0.1～20w/w%.
9. method according to claim 4, it is characterised in that the preparation method of described Glomus intraradices microbial inoculum is as follows:
Inoculum concentration according to 0.1～20w/w%, by evenly laid out for Glomus intraradices Inoculant in sandy soil substrate, cover lid layer sterilizing sandy soil substrate, water, the seed of sowing Glomus intraradices host, cover lid layer sterilizing sandy soil substrate again, cultivate 3～6 months, after removing above-ground plant parts stem and leaf, it is placed in the stable space of temperature and humidity to dry 10～15 days, then gathers in the crops all cultures, including plant root segment, mycelia, mycorrhizal fungal spore and cultivation matrix, mince mixing, namely obtain Glomus intraradices microbial inoculum.
10. the soil conditioner being applicable to plant Flos Lonicerae on cadmium pollution soil, it is characterised in that include Glomus intraradices.
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| CN111001655A (en) * | 2019-12-26 | 2020-04-14 | 青岛冠中生态股份有限公司 | Method for repairing cadmium-polluted soil by using arbuscular mycorrhizal fungi and torch trees |
| CN112893453A (en) * | 2021-03-31 | 2021-06-04 | 华南师范大学 | Method for repairing cadmium-polluted soil by intercropping corn and wedelia chinensis and inoculating AMF |
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| CN106912349A (en) * | 2017-03-01 | 2017-07-04 | 济南大学 | A kind of method that utilization fungi promotes galuteolin accumulation in In Flower Buds of Lonicera Japonica Thunb |
| CN106912349B (en) * | 2017-03-01 | 2020-03-13 | 济南大学 | Method for promoting accumulation of luteoloside in honeysuckle flower buds by using fungi |
| CN109006242A (en) * | 2018-07-24 | 2018-12-18 | 贵州理工学院 | Between the practice joint AM fungi method that reduces cadmium, Zn content in capsicum and soil |
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| CN112893453A (en) * | 2021-03-31 | 2021-06-04 | 华南师范大学 | Method for repairing cadmium-polluted soil by intercropping corn and wedelia chinensis and inoculating AMF |
| CN113207567A (en) * | 2021-05-25 | 2021-08-06 | 上海交通大学 | Method for symbiotic growth and mildew activity determination of rhizosporangium in cyperus esculentus and plant roots |
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