CN104928191A - Arbuscular mycorrhizal fungi GZ176 for disease prevention, growth promotion and adverse resistance of herbaceous plants and microbial inoculums and application of arbuscular mycorrhizal fungi GZ176 - Google Patents
Arbuscular mycorrhizal fungi GZ176 for disease prevention, growth promotion and adverse resistance of herbaceous plants and microbial inoculums and application of arbuscular mycorrhizal fungi GZ176 Download PDFInfo
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Abstract
The invention discloses arbuscular mycorrhizal fungi GZ176 for disease prevention, growth promotion and adverse resistance of herbaceous plants and microbial inoculums and application of the arbuscular mycorrhizal fungi GZ176. The arbuscular mycorrhizal fungi GZ176 is rhizophagus intraradices and can be made into powder with concentration being 40-60 spores per gram, herbaceous plant disease prevention rate is up to 71.3% after the arbuscular mycorrhizal fungi GZ176 powder is subjected to seed dressing under normal management conditions of greenhouses, and herbaceous plant growth promotion rate is higher than 16.19%; in the field, disease prevention and control rate reaches 75.27%, and yield increasing rate exceeds 36.17%; adverse resistance of the herbaceous plants can be improved remarkably.
Description
Technical field
The invention belongs to plant protection art, relate to for herbaceous plant diseases prevention, growth-promoting, degeneration-resistant bush mycorrhizal fungi GZ176 and microbial inoculum thereof and application.
Background technology
China is large agricultural country, China herbaceous plant (comprises the farm crop such as paddy rice, wheat, tomato, capsicum, eggplant, tobacco, cucumber, watermelon, muskmelon, celery, the horticulture and flower such as chrysanthemum, turfgrass, and the Chinese medicinal materials etc. such as pseudo-ginseng) produce the total area more than 2,200,000,000 mu, for tremendous contribution has been made in world agriculture development.But, China be also disease and pest multiple, take place frequently and retransmit big country, disease and pest and floods, drought be called China three the Nature disaster.China's common disease and pest has more than 1000 kinds, can cause nearly 100 kinds of the great disease and pest of serious harm throughout the year, and annual occurring area is more than 7,000,000,000 mu times, and often kind of crop is endangered by the sick worm of 3-4 kind often simultaneously.Food and Argriculture OrganizationFAO estimates, the crop loss that the annual disease and pest of China causes reaches 70,000,000,000-900 hundred million yuan, and wherein disease accounts for 33%, and crop pest becomes the important factor of restriction China agroforestry production Sustainable development just increasingly.
Herbaceous plant disease is bag soil-borne disease and overground part disease mainly.Plant soil-borne diseases causes the illness of plant root and even complete stool property usually, it occurs and popularly usually belongs to popular disease for many years, the diseases such as line is withered, withered, Huang withers as caused, it is withered to stand, damping off, root-rot, green grass or young crops withered, soft corruption, root knot nematode, Cyst nematode, knee.The position on the ground such as overground part disease main harm blade, stem, fruit, mainly importing into outside greenhouse by the farming activities of air-flow and people, as oidium, anthrax, rust, gray mold etc.These disease pathogens have that host range is wide, strong stress resistance, be difficult to the features such as elimination.
Poor environment comprises the severe weathers such as arid, flood, high temperature, cold, all can produce detrimentally affect to the growth of herbaceous crops.The nineties in 20th century, China every year on average area suffered from drought is 2,733 ten thousand hm
2, grain reaches 4.7% because of non-irrigated output reduction and the ratio of ultimate production.And every year because of waterlogged disaster loss grain 62.3 hundred million kg, average annual financial loss 46.7 hundred million yuan.The weather such as high temperature, cold also can cause serious harm to crop production.Increase herbaceous crops and resist the great attention that extraneous poor environment ability receives domestic and international investigator, current effective means is considerably less.
Utilize soil beneficial microorganism to control the generation of disease and to induce herbaceous crops anti-adversity ability to be the focus studied in the last few years, especially these microorganism environmentally safes, cause scientists and utilize biological control means to control the interest of disease.The root growth symbiotic relationship of bush mycorrhizal fungi (arbuscular mycorrhizal fungi, hereinafter referred to as AM fungi) energy and most of terrestrial plant, it has very important effect to ecotope.It can by different way or approach affect many metabolic processes of plant, increase roots of plants long, change plant root species form to build up, promote that root system is to the absorption of moisture content and nutritive element, AM fungi can improve herbal resistance against diseases, infecting of opposing pathogenic bacteria, and anti-adversity ability, strengthen coercing of the environment such as plant reply cold, salinity, arid.
CN103155815 discloses a kind of method improving tobacco bacterial wilt resistance, comprise and nectarine Acaulospora (Acaulosporamellea), Glomus mosseae (Glomusmosseae), Glomus intraradices (Glomusintraradices) expanded separately numerous; Expand numerous after nectarine Acaulospora (Acaulosporamellea), Glomus mosseae (Glomusmosseae), Glomus intraradices (Glomusintraradices) four kinds of bush mycorrhizal fungis are according to 0.5-1:1:1, weight ratio is mixed with mixing bush mycorrhizal fungi preparation and infects tobacco, this microbial inoculum can promote tobacco growing, the sickness rate of the soil-borne disease of effective reduction tobacco and disease index, thus improve the resistance of tobacco to disease.But the bush mycorrhizal fungi combined action that this patent utilization four kinds is different, and synergy between bacterium, may be there is, those skilled in the art cannot expect to use wherein a kind of bush mycorrhizal fungi whether still can play growth-promoting and diseases prevention effect.And the bacterial strain of different sources may have different physio-biochemical characteristics and biological function, even if different plants uses identical microbial inoculum also may produce different effects.Which strain bush mycorrhizal fungi can produce desirable biocontrol effect to herbal soil-borne disease and ground disease actually, and improves herbal resistance, has not yet to see relevant report.
Summary of the invention
The object of the invention is for the deficiencies in the prior art, what provide a kind of environmental protection can improve herbaceous plant diseases prevention, growth-promoting, degeneration-resistant bush mycorrhizal fungi GZ176.
Another object of the present invention is to provide the pulvis of this bush mycorrhizal fungi GZ176
Object of the present invention realizes by following technical scheme:
For herbaceous plant diseases prevention, growth-promoting, degeneration-resistant bush mycorrhizal fungi GZ176, this strain classification called after Glomus intraradices (Rhizophagus intraradices), be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on November 25th, 2014, culture presevation number is CGMCC NO.10056.
The application of bush mycorrhizal fungi of the present invention in the diseases prevention of preparation herbaceous plant, growth-promoting, degeneration-resistant preparation; Preferably in preparation control farm crop, horticulture and flower, the herbal soil-borne disease of Chinese medicinal materials and overground part disease, promote that it grows, improve the application in the preparation of its quality and/or resistance; Any one in the wherein said preferred paddy rice of farm crop herbaceous plant, wheat, Solanaceae, Curcurbitaceae, leaf vegetables, the described preferred composite family of horticulture and flower herbaceous plant etc.; The described preferred snakegourd of Chinese medicinal materials herbaceous plant, the tuber of pinellia etc.; The preferred drought resistance of described resistance.
With the microbial inoculum prepared for herbaceous plant diseases prevention, growth-promoting, degeneration-resistant bush mycorrhizal fungi GZ176 of the present invention.
Described microbial inoculum prepares preferably by following methods:
1) by containing described concentration be the soil of the mycorrhizal fungi GZ176 spore of 40-60 spore/g by the volume ratio of 1:80-120 and culture medium Homogeneous phase mixing, substrate formula: peat soil mixes with the volume ratio through the steamed coarse sand 1:0.5-3 of at least 2h hot gas;
2) will the plastic tub loading sterilizing containing the matrix of spore be mixed, and plant upper host plant, ensure that spore can touch with Chinese sorghum very fully; After 4 months, by the soil in basin alms bowl and host plant root system one piece taking-up, dry, pulverize, keep dry, the obtained soil containing mycorrhizal fungal spore, for subsequent use; The preferred Chinese sorghum of described host plant or trifolium;
3) by step 2) the obtained soil containing mycorrhizal fungal spore mix with kaolin, and dry rear pulverizing, again drying to water content is 3%, and final mistake 200 mesh sieve, makes the pulvis that concentration is 40-60 spore/g.
Described microbial inoculum prepares preferably by following methods further:
1) soil containing the mycorrhizal fungi GZ176 spore described in claim 1 is pressed volume ratio and the culture medium Homogeneous phase mixing of 1:80-120, substrate formula: peat soil mixes with the coarse sand 1:0.5-3 steamed through at least 2h hot gas;
2) plastic tub loading sterilizing containing the matrix of spore will be mixed, plastic tub diameter 40cm, volume 0.05m
3, 50-60 sorghum seeds in each basin alms bowl kind, ensures that spore can touch with Chinese sorghum very fully; After 4 months, by the soil in basin alms bowl and Sorghum roots one piece taking-up, dry, be crushed to 800 orders, keep dry, the obtained soil containing mycorrhizal fungal spore, for subsequent use;
3) prepare: by step 2) the obtained soil containing mycorrhizal fungal spore mixes with kaolin, and be crushed to 200 orders after oven dry, again drying to water content is 3%, and final mistake 200 mesh sieve, makes the pulvis that concentration is 40-60 spore/g.
The preparation method of microbial inoculum of the present invention, is characterized in that comprising the following steps:
1) by containing described concentration be the soil of the mycorrhizal fungi GZ176 spore of 40-60 spore/g by the volume ratio of 1:80-120 and culture medium Homogeneous phase mixing, substrate formula: peat soil mixes with the volume ratio through the steamed coarse sand 1:0.5-3 of at least 2h hot gas;
2) will the plastic tub loading sterilizing containing the matrix of spore be mixed, and plant upper host plant, ensure that spore can touch with Chinese sorghum very fully; After 4 months, by the soil in basin alms bowl and host plant root system one piece taking-up, dry, pulverize, keep dry, the obtained soil containing mycorrhizal fungal spore, for subsequent use; The preferred Chinese sorghum of described host plant or trifolium;
3) by step 2) the obtained soil containing mycorrhizal fungal spore mix with kaolin, and dry rear pulverizing, again drying to water content is 3%, and final mistake 200 mesh sieve, makes the pulvis that concentration is 40-60 spore/g.
Bush mycorrhizal fungi of the present invention herbaceous plant diseases prevention, growth-promoting, degeneration-resistant in application; Preferably in control farm crop, horticulture and flower, the herbal soil-borne disease of Chinese medicinal materials and overground part disease, promote that it grows, improve the application in its quality and/or resistance; Any one in the wherein said preferred paddy rice of farm crop herbaceous plant, wheat, Solanaceae, Curcurbitaceae, leaf vegetables, the described preferred composite family of horticulture and flower herbaceous plant etc.; The described preferred snakegourd of Chinese medicinal materials herbaceous plant, the tuber of pinellia; The preferred drought resisting of described resistance, cold-resistant, heat-resisting, waterlogging, opposing bad climate; The preferred cold spell in later spring of bad climate.
Described microbial inoculum is applied preferably by mixing matrix treatments.
Beneficial effect
The present invention utilizes and prevents and treats herbaceous plant soil-borne disease and overground part disease to the microbiobacterial agent of environment toxicological harmless, provide a kind of bush mycorrhizal fungi GZ176 pulvis or liquid preparation of environmental protection, paddy rice can be prevented and treated, wheat, Solanaceae, Curcurbitaceae, the medium farm crop of leaf vegetables, the horticulture and flowers such as chrysanthemum, and snakegourd, all herbal soil-borne diseases such as the tuber of pinellia and overground part disease, promote the growth of above-mentioned all kinds of crop and improve quality and improve its resistance (to comprise drought resisting, cold-resistant, heat-resisting, waterlogging etc., the bad climates such as opposing cold spell in later spring), solve other method poor effect such as chemical agent or have the problems such as residual, protection of the environment, promoting agricultural sustainable development.
Said preparation to bush mycorrhizal fungi GZ176 pulvis mix soil process after to disease biocontrol effect up to 71.3%, act on fairly obvious; And to the plant height of plant and root long have improve significantly, to plant, there is certain growth-promoting functions, through the herbaceous plant of GZ176 process compared with control group, increase production and surpass 17.75%.This mycorrhizal fungi GZ176 pulvis, can room temperature preservation 6 months-1 year.The field growth-promoting test-results of AM fungi preparation process shows, preventive effect, up to 75.27%, directly saves nearly 20000 yuan of every mu, peasant household financial loss.
Accompanying drawing explanation
The process of Fig. 1, GZ176 pulvis is to the biocontrol effect of herbaceous crops disease
The process of Fig. 2, GZ176 pulvis is on the impact of herbaceous crops biomass
The process of Fig. 3, GZ176 pulvis is on the impact of herbaceous crops resistance
Biological deposits information
GZ176, is Glomus intraradices (Rhizophagus intraradices).Be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on November 25th, 2014, culture presevation number is CGMCC NO.10056, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City institute of microbiology of the Chinese Academy of Sciences.
Embodiment
Embodiment 1
Improve herbaceous crops biological control of diseases effect and growth-promoting, a degeneration-resistant bush mycorrhizal fungi GZ176 pulvis, containing microbe composition: bush mycorrhizal fungi GZ176 (preserving number is CGMCC NO.10056).Obtain by being separated in Guizhou Province's tobacco rhizosphere soil.
The separation of AM mycorrhizal fungi GZ176, authentication method: pour into-sucrose centrifuging separation of AM fungal spore with wet screening, take 100g soil sample in centrifuge tube, add water to centrifuge tube 2/3 place, supernatant liquor is removed after in the centrifugal 3min of 3000rpm, add the sucrose solution of 45%-50%, stir evenly the centrifugal 1.5min of rear rapid 1500rpm, cross 400 mesh sieves, and rinse spore above sieve and sporocarp gently with water, be collected in culture dish with physiological saline, add up spore number under the microscope and observe spore shape.
GZ176 morphological feature: conidial cell wall mostly is 3-4 layer, outer water white transparency, 1-2 μm, is easily separated with inwall.Inside there is the faint yellow wall of layer 2-3, each 1-1.5 μm.Only connect a place and connect an armful mycelia wall multilayer, be connected with an armful wall, an armful wall forms flamboyancy when the company's of entering point tubaeform.In Melzer reagent, embrace sub-scarlet, easily dye blueness at the blue reagent mesectoderm of cotton, internal layer is light blue, painted slightly slow.Embrace son mostly in root, root also has outward sometimes.It is consistent with the form in root that root embraces son outward, but slightly large.It is transparent that children embraces son age, and 2 layers of wall uniform thickness, about 1 μm, aged person's skin is transparent, internal layer tawny.Be Glomus intraradices (Rhizophagusintraradices) through Morphological Identification, deliver the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, culture presevation number is CGMCC NO.10056.
Embodiment 2
The preparation method of AM fungus G Z176 pulvis is as follows:
1) numerous soil containing bush mycorrhizal fungi GZ176 will be expanded in the past by the volume ratio of 1:100 and culture medium Homogeneous phase mixing, substrate formula: peat soil mixes with the coarse sand 1:1 steamed through at least 2h hot gas;
2) matrix mixed containing spore is loaded geobasin alms bowl (diameter 15cm) after sterilizing, and plant upper Chinese sorghum, each basin alms bowl 50-60 seed, ensure that spore can touch with Chinese sorghum very fully.After 4 months, by the soil in basin alms bowl and Sorghum roots one piece taking-up, dry, be crushed to 800 orders, keep dry, the obtained soil containing mycorrhizal fungal spore, for subsequent use;
3) preparation: according to step 2) in the soil of mycorrhizal fungal spore mix with kaolin, pulverize after drying, cross 300 mesh sieves, again drying to water content is 3%, finally makes the pulvis that concentration is about 50 spore/g.
The diseases prevention test of embodiment 3 greenhouse
Method: will participate in the experiment tomato seeds 10%H
2o
2surface sterilization 10min, sterilized water dries for subsequent use after cleaning.
Before sowing, the 200g sterile soil in each basin alms bowl after dress mixing and 10g GZ176 pulvis (prepared by embodiment 2), to ensure normal inoculation.Each nutrition pot sows 10 seeds, normally waters management after 4 weeks, transplants Va Mycorrhiza Seedling in 330ml plastic cup, selects the consistent plant of growing way to carry out experiment during transplanting.Often organize process 24 young plant, place at random, altogether in triplicate.
The former bacterium ZJ3721 of bacterial wilt (tomato plant that bacterial wilt occurs from Zhejiang is separated and obtains, and preserves) is inoculated after transplanting 3d.Dull and stereotyped activation Ralstonia solanacearum ZJ3721, spends the night in 500ml YGPA nutrient solution and shakes bacterium (200rpm/min, 20-24h, 30 DEG C).Use 0.85%Nacl solution bacterial concentration to be adjusted to 1 × 107CFU/mL subsequently and carry out root irrigation, every strain tomato seedling waters the Ralstonia solanacearum liquid getting 20ml.Investigate incidence every day afterwards, by bacterial wilt graded index, carry out disease index statistics, greenhouse experiments result is investigated.After inoculation, every day investigates incidence, and by bacterial wilt feelings graded index, every strain carries out disease index statistics by leaflet, investigates greenhouse test.
According to Kempe (1983) (Kempe, J., and Sequeira, L.1983.Biological control of baterial wilt ofpotatoes:Attempts to induce resistance by treating tubes with bacteria.Plant Disease, 67 (5): 499-501.) the sick progression of the sick grade standard record proposed, severity Scaling:
0 grade: asymptomatic;
There is wilting symptom in 1 grade: 1-25% blade;
There is wilting symptom in the blade of 2 grades: complete stool 26%-50%;
3 grades: wilting appears in the blade of complete stool 51% one 75%;
There is wilting symptom in the blade of 4 grades: complete stool 76%-100%.
Preventive effect (%)=[(contrast disease severity one processes disease severity)/contrast disease severity] × 100%
Disease severity=[∑ (each sick level strain number × each disease index)/(investigating total strain number × green grass or young crops the highest withered disease index)] × 100
Experimental result is as table 1, and in greenhouse production, the prevention effect of AM fungi to bacterial wilt of tomato reaches more than 71.3%.
The process of table 1GZ176 pulvis is to the biocontrol effect of bacterial wilt of tomato
The greenhouse growth-promoting test of embodiment 4GZ176 pulvis process
Method: tomato of participating in the experiment, cucumber seeds 10%H
2o
2surface sterilization 10min, sterilized water dries for subsequent use after cleaning.Each nutrition pot sows 10 seeds.Normally water management after 4 weeks, transplants Va Mycorrhiza Seedling in 330ml plastic cup, the 200g sterile soil in each plastic cup after dress mixing and 10g GZ176 pulvis (prepared by embodiment 2), to ensure normal inoculation.Select the consistent plant of growing way to carry out experiment during transplanting, often organize process 48 young plant, place at random, altogether in triplicate.Tomato, cucumber plant biomass is measured after 30d.
To be participated in the experiment paddy rice, wheat, mater convolvulus seed 10%H
2o
2surface sterilization 10min, sterilized water dries for subsequent use after cleaning.200g sterile soil in each basin alms bowl (diameter 9cm, high 13cm) after dress mixing and 10g GZ176 pulvis (prepared by embodiment 2), to ensure normal inoculation.Each nutrition pot sows 4 seeds, often organizes process 48 young plant, places at random, altogether in triplicate.Not add the process of GZ176 pulvis in contrast.Paddy rice, wheat, mater convolvulus biomass is measured after inoculation 30d.
Using the chrysanthemum of participating in the experiment from the tender tip of the long 12cm of clip the shoot that perennial root (old root) is sprouted as cutting, scabble from joint base portion, cut the two panels great Ye of base portion, again the leaf on top is repaiied half and (reduce moisture evaporation, prevent cutting from wilting) get final product cuttage to (diameter 9cm, high 13cm) in basin alms bowl.200g sterile soil in each basin alms bowl after dress mixing and 10g GZ176 pulvis (prepared by embodiment 2), to ensure normal inoculation.Not add the process of GZ176 pulvis in contrast.Often organize process 48 young plant, place at random, altogether in triplicate.Biomass is measured after inoculation 30d.
As shown in table 2,3,4,5,6,7, the process of GZ176 pulvis is thick to the plant height of Solanaceae tomato, Curcurbitaceae cucumber, paddy rice, wheat, leafy vegetable mater convolvulus, chrysanthemum, stem, blade area, fresh dry weight have the effect of significantly improving, and growth-promoting effect reaches 16.19%, 38.83%, 26.72%, 45.19%, 50.88%, 20.33% respectively.
The process of table 2GZ176 pulvis is on the impact of tomato biological amount
Note: different lowercase person represents significant difference in 0.05 level.
The process of table 3GZ176 pulvis is on the impact of cucumber biomass
Note: different lowercase person represents significant difference in 0.05 level.
The process of table 4GZ176 pulvis is on the impact of rice biological amount
Note: different lowercase person represents significant difference in 0.05 level.
The process of table 5GZ176 pulvis is on the impact of wheat biomass
Note: different lowercase person represents significant difference in 0.05 level.
The process of table 6GZ176 pulvis is on the impact of mater convolvulus biomass
Note: different lowercase person represents significant difference in 0.05 level.
The process of table 7GZ176 pulvis is on the impact of chrysanthemum biomass
Note: different lowercase person represents significant difference in 0.05 level.
Field biological and ecological methods to prevent plant disease, pests, and erosion and the growth-promoting of the process of embodiment 5GZ176 powder preparation are tested
Method: two process: A are established in this test altogether: tomato " Te Lina " (buying in Nanjing Shou De experiment equipment company limited)+GZ176 pulvis (prepared by embodiment 2): during nursery, be sowed in the dish of cave after GZ176 pulvis is dressed seed, during to 4 leaf phases (about 4 weeks), by Mycorrhizal transplantation of seedlings to large Tanaka; B: to dress seed as blank without GZ176 pulvis.Often process establishes 3 communities to repeat, the group arrangement of each process random district, to protect row isolation between community.Other aspects, experimental plot manage routinely.
At the slow seedling of transplanting after 30 days, statistics disease severity, after 40 days, every community is selected 24 homogeneous strain tomatoes of growing way and is measured the thick and number of blade of plant heights, stem.The 10 strain tomatoes that during results, growing way is selected homogeneous in each community measure output.
According to table 8, after the process of GZ176 pulvis, the disease severity of bacterial wilt of tomato controls 17.16%, and namely preventive effect is up to 75.27%, directly saves nearly 20000 yuan of every mu, peasant household financial loss.As shown in Table 9, after " Te Lina " transplants 40 days, the thick and number of blade of the plant height for the treatment of group tomato, stem has and is significantly better than control group.
The process of GZ176 pulvis can make tomato soluble proteins content raising 25.4%, soluble sugar content improves 75.6%, Vc content and improves 54%, and content of lycopene improves 59.58%.Because the generation of bacterial wilt is serious, cause control group almost to have no harvest, the tomato per mu yield through the process of GZ176 pulvis is 121.73kg, and compared with control group, volume increase surpasses 177.54% (table 10).
Table 8GZ176 pulvis is to the protection effect (transplanting latter 30 days) of tomato " Te Lina "
Note: different lowercase person represents significant difference in 0.05 level.
Table 9GZ176 pulvis is to the growth-promoting effect (transplanting latter 40 days) of tomato " Te Lina "
Note: different lowercase person represents significant difference in 0.05 level.
Table 10GZ176 pulvis is to the quality of tomato " Te Lina " fruit and yield effect
Note: different lowercase person represents significant difference in 0.05 level.
The induction herbaceous crops tuber of pinellia of embodiment 6GZ176 powder preparation process, the test of snakegourd field disease prevention growth-promoting
Method: before tuber of pinellia bulb of participating in the experiment sowing, dress seed according to the amount of 10g pulvis/Kg bulb, reseeds in continuous cropping field (bacterial soft rot morbidity is serious).Not add the process of GZ176 pulvis in contrast.Each community 10m
3, repeat for 3 times.Management process management routinely, when summer gathers in the crops, adds up output and the bacterial soft rot sickness rate of every square metre.
Before snakegourd block root of participating in the experiment sowing, dress seed according to the amount of 10g pulvis/Kg block root, reseed in continuous cropping field (root knot nematode morbidity is serious).Not add the process of GZ176 pulvis in contrast.Each community 20m
3, repeat for 3 times.Management process management routinely, when autumn gathers in the crops, adds up each community snakegourd fruit number and root knot nematode morbidity sickness rate.
Sickness rate (%)=[disease plant number/(disease plant number+healthy plant number)] × 100%
As shown in table 11, the output of the GZ176 pulvis treatment group tuber of pinellia every square metre is significantly higher than control group, and reach 68.45% to the preventive effect of bacterial soft rot, effect of increasing production reaches 36.17%.After GZ176 pulvis process snakegourd, 56.27% is reached to root knot nematode preventive effect also, volume increase 46.69% (table 12).
The effect of table 11GZ176 pulvis on the tuber of pinellia
Note: different lowercase person represents significant difference in 0.05 level.
The effect of table 12GZ176 pulvis on snakegourd
Note: different lowercase person represents significant difference in 0.05 level
The induction herbaceous crops tobacco resistance test of embodiment 7GZ176 powder preparation process
Method: will participate in the experiment tobacco seed 10%H
2o
2surface sterilization 10min, sterilized water dries for subsequent use after cleaning.
Before sowing, the 200g sterile soil in each basin alms bowl after dress mixing and 10g GZ176 pulvis, to ensure normal inoculation.Each nutrition pot sows 10 seeds, normally waters management after 6 weeks, transplants Va Mycorrhiza Seedling in 330ml plastic cup, select the plant that growing way is consistent during transplanting, carry out cutting off the water supply arid after 1 month, drought stress continues 15d, normally water after drought stress, observe the restoration ecosystem situation of tobacco seedling.Often organize process 24 young plant, place at random, altogether in triplicate.Put according to randomized complete-block design in greenhouse.
Water after carrying out restoration ecosystem 6d, GZ176 pulvis treatment group plant restore normal growth, and and control group plant all dead (table 13).
Table 13GZ176 pulvis is on the impact of tobacco drought tolerance
Note: different lowercase person represents significant difference in 0.05 level.
Claims (10)
1. for herbaceous plant diseases prevention, growth-promoting, degeneration-resistant bush mycorrhizal fungi GZ176, this strain classification called after Glomus intraradices (Rhizophagus intraradices), be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on November 25th, 2014, culture presevation number is CGMCC NO.10056.
2. the application of bush mycorrhizal fungi according to claim 1 in the diseases prevention of preparation herbaceous plant, growth-promoting, degeneration-resistant preparation.
3. application according to claim 2, it is characterized in that bush mycorrhizal fungi GZ176 according to claim 1 is in herbal soil-borne disease and overground part diseases such as preparation control farm crop, horticulture and flower, Chinese medicinal materialss, promote that it grows, improve the application in the preparation of its quality and/or resistance; Any one in the wherein said preferred paddy rice of farm crop herbaceous plant, wheat, Solanaceae, Curcurbitaceae, leaf vegetables, the described preferred composite family of horticulture and flower herbaceous plant; The described preferred snakegourd of Chinese medicinal materials herbaceous plant or the tuber of pinellia; The preferred drought resistance of described resistance.
4. with microbial inoculum prepared by bush mycorrhizal fungi GZ176 according to claim 1.
5. microbial inoculum according to claim 4, is characterized in that preparing by the following method:
1) by containing the concentration described in claim 1 be the soil of the mycorrhizal fungi GZ176 spore of 40-60 spore/g by the volume ratio of 1:80-120 and culture medium Homogeneous phase mixing, substrate formula: peat soil mixes with the volume ratio through the steamed coarse sand 1:0.5-3 of at least 2h hot gas;
2) will the plastic tub loading sterilizing containing the matrix of spore be mixed, and plant upper host plant, ensure that spore can touch with Chinese sorghum very fully; After 4 months, by the soil in basin alms bowl and host plant root system one piece taking-up, dry, pulverize, keep dry, the obtained soil containing mycorrhizal fungal spore, for subsequent use; The preferred Chinese sorghum of described host plant or trifolium;
3) by step 2) the obtained soil containing mycorrhizal fungal spore mix with kaolin, and dry rear pulverizing, again drying to water content is 3%, and final mistake 200 mesh sieve, makes the pulvis that concentration is 40-60 spore/g.
6. microbial inoculum according to claim 5, is characterized in that preparing by the following method:
1) soil containing the mycorrhizal fungi GZ176 spore described in claim 1 is pressed volume ratio and the culture medium Homogeneous phase mixing of 1:80-120, substrate formula: peat soil mixes with the coarse sand 1:0.5-3 steamed through at least 2h hot gas;
2) plastic tub loading sterilizing containing the matrix of spore will be mixed, plastic tub diameter 40cm, volume 0.05m
3, 50-60 sorghum seeds in each basin alms bowl kind, ensures that spore can touch with Chinese sorghum very fully; After 4 months, by the soil in basin alms bowl and Sorghum roots one piece taking-up, dry, be crushed to 800 orders, keep dry, the obtained soil containing mycorrhizal fungal spore, for subsequent use;
3) prepare: by step 2) the obtained soil containing mycorrhizal fungal spore mixes with kaolin, and be crushed to 200 orders after oven dry, again drying to water content is 3%, and final mistake 200 mesh sieve, makes the pulvis that concentration is 40-60 spore/g.
7. the preparation method of microbial inoculum according to claim 4, is characterized in that comprising the following steps:
1) by containing the concentration described in claim 1 be the soil of the mycorrhizal fungi GZ176 spore of 40-60 spore/g by the volume ratio of 1:80-120 and culture medium Homogeneous phase mixing, substrate formula: peat soil mixes with the volume ratio through the steamed coarse sand 1:0.5-3 of at least 2h hot gas;
2) will the plastic tub loading sterilizing containing the matrix of spore be mixed, and plant upper host plant, ensure that spore can touch with Chinese sorghum very fully; After 4 months, by the soil in basin alms bowl and host plant root system one piece taking-up, dry, pulverize, keep dry, the obtained soil containing mycorrhizal fungal spore, for subsequent use; The preferred Chinese sorghum of described host plant or trifolium;
3) by step 2) the obtained soil containing mycorrhizal fungal spore mix with kaolin, and dry rear pulverizing, again drying to water content is 3%, and final mistake 200 mesh sieve, makes the pulvis that concentration is 40-60 spore/g.
8. microbial inoculum described in claim 5 or 6 herbaceous plant diseases prevention, growth-promoting, degeneration-resistant in application.
9. application according to claim 8, it is characterized in that, microbial inoculum described in claim 5 or 6, in control farm crop, horticulture and flower, the herbal soil-borne disease of Chinese medicinal materials and overground part disease, promotes that it grows, improves the application in its quality and/or resistance; Any one in the wherein said preferred paddy rice of farm crop herbaceous plant, wheat, Solanaceae, Curcurbitaceae, leaf vegetables, the described preferred composite family of horticulture and flower herbaceous plant; The described preferred snakegourd of Chinese medicinal materials herbaceous plant or the tuber of pinellia; The preferred drought resistance of described resistance.
10. application according to claim 8 or claim 9, it is characterized in that, described microbial inoculum is by mixing matrix treatments.
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