CN104604557A - Application and method for increasing cotton yield through AM fungus infestation of cotton - Google Patents
Application and method for increasing cotton yield through AM fungus infestation of cotton Download PDFInfo
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- CN104604557A CN104604557A CN201510037602.9A CN201510037602A CN104604557A CN 104604557 A CN104604557 A CN 104604557A CN 201510037602 A CN201510037602 A CN 201510037602A CN 104604557 A CN104604557 A CN 104604557A
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- 229920000742 Cotton Polymers 0.000 title claims abstract description 112
- 241000233866 Fungi Species 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 26
- 206010061217 Infestation Diseases 0.000 title abstract 3
- 241000219146 Gossypium Species 0.000 claims abstract description 106
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 46
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 25
- 239000011574 phosphorus Substances 0.000 claims abstract description 25
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 25
- 240000002024 Gossypium herbaceum Species 0.000 claims abstract description 24
- 235000004341 Gossypium herbaceum Nutrition 0.000 claims abstract description 24
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 23
- 239000007788 liquid Substances 0.000 claims abstract description 18
- 239000003337 fertilizer Substances 0.000 claims abstract description 15
- 235000012343 cottonseed oil Nutrition 0.000 claims abstract description 8
- 239000004576 sand Substances 0.000 claims abstract description 8
- 206010017533 Fungal infection Diseases 0.000 claims description 22
- 208000031888 Mycoses Diseases 0.000 claims description 22
- 239000002068 microbial inoculum Substances 0.000 claims description 22
- 235000016709 nutrition Nutrition 0.000 claims description 17
- 230000035764 nutrition Effects 0.000 claims description 17
- 241000235504 Rhizophagus intraradices Species 0.000 claims description 13
- 241000894006 Bacteria Species 0.000 claims description 12
- 239000002131 composite material Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000005286 illumination Methods 0.000 claims description 7
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 6
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 claims description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 3
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 3
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 3
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 3
- 239000004327 boric acid Substances 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 3
- 229910000366 copper(II) sulfate Inorganic materials 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims description 3
- 239000011565 manganese chloride Substances 0.000 claims description 3
- 235000002867 manganese chloride Nutrition 0.000 claims description 3
- 229940099607 manganese chloride Drugs 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 3
- 239000001103 potassium chloride Substances 0.000 claims description 3
- 235000011164 potassium chloride Nutrition 0.000 claims description 3
- 239000011684 sodium molybdate Substances 0.000 claims description 3
- 235000015393 sodium molybdate Nutrition 0.000 claims description 3
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 3
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 claims description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 3
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 3
- 239000011686 zinc sulphate Substances 0.000 claims description 3
- 235000009529 zinc sulphate Nutrition 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 abstract description 6
- 238000005516 engineering process Methods 0.000 abstract description 4
- 238000012271 agricultural production Methods 0.000 abstract description 2
- 239000002686 phosphate fertilizer Substances 0.000 abstract description 2
- 150000001875 compounds Chemical class 0.000 abstract 1
- 239000002054 inoculum Substances 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 18
- 239000002689 soil Substances 0.000 description 9
- 208000015181 infectious disease Diseases 0.000 description 6
- 238000010521 absorption reaction Methods 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 208000035240 Disease Resistance Diseases 0.000 description 3
- 241001123597 Funneliformis mosseae Species 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 241000773655 Ambispora fennica Species 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 241001269238 Data Species 0.000 description 1
- 241000235502 Gigaspora margarita Species 0.000 description 1
- 241000235503 Glomus Species 0.000 description 1
- 241000214030 Rhizophagus fasciculatus Species 0.000 description 1
- 241000082085 Verticillium <Phyllachorales> Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000010425 asbestos Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
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- 230000000694 effects Effects 0.000 description 1
- 238000013213 extrapolation Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 229940085805 fiberall Drugs 0.000 description 1
- 239000002783 friction material Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 229910052895 riebeckite Inorganic materials 0.000 description 1
- 239000002688 soil aggregate Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- -1 stem Polymers 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The invention provides an application and method for increasing the cotton yield through AM fungus infestation of cotton and belongs to the field of AM fungus technology. The method comprises the steps that cotton seeds are placed into a cultivation box containing river sand for seedling culture; after the cotton seeds germinate and grow four leaves, arbuscular mycorrhizal fungus liquid inoculants are added to the roots of all cotton plants, and a nitrogen-limited phosphorus-limited nutrient solution is applied once every seven days during cultivation; the cotton plants are transplanted into cultivation pots containing sand; the nitrogen-limited phosphorus-limited nutrient solution is applied once every three days after the cotton plants are cultivated for ten days; after seedling cultivation is conducted for 40 days, the cotton plants are transplanted to a test field; after the cotton plants are transplanted to the test field for 30 days, compound fertilizer is applied to the roots of the cotton plants, and the cotton plants are watered every 15 days; after three months, the cotton is harvested. According to the method, the phosphorus content of the applied nutrient solution is very low, and the nitrogen content of the applied nutrient solution is high; in agricultural production application, application of phosphate fertilizer can be reduced, and accordingly the infestation rate of AM fungi and the cotton and the cotton yield are increased.
Description
Technical field
The invention belongs to AM fungi technical field, be specifically related to application and method thereof that AM fungal infection cotton improves output of cotton.
Background technology
Mycorhiza is the symbiont that root system of plant and mycorrhizal fungi are formed, and the arbuscular mycorrhizal fungi ubiquitous class mycorrhizal fungi that is occurring in nature, on the earth 80% terrestrial plant can with its formation arbuscular mycorrhizal fungi syntaxial system.The Primary Reference point that arbuscular mycorrhizal fungi and plant form syntaxial system is exactly the p of E that AM fungi enters the position in root.Meanwhile, unit root length p of E quantity can be used as one of parameter measuring AM fungal infection gesture size.After the microbial inoculum inoculation plant of i.e. a kind of AM fungi, the p of E quantity formed illustrate more at most this microbial inoculum to infect gesture larger.When the mycelia of AM fungi and plant large area infect, a large amount of extension of the outer mycelia of root of its growth and expansion and mycorhiza infect and can increase root system branches quantity and amount of growth, the scope of nutrient needed for the absorption of increase root system of plant, improves plant to the utilization ratio of effective nutritive element.Meanwhile, mycelia has important function to the formation of soil aggregate structure, the aspect such as stability and maintenance soil porosity.Therefore, compared with other edaphons, mycorrhizal fungi is in occupation of the ecologic niche of uniqueness.
Cotton belongs to the Malvaceae Gossypium in terrestrial plant, is the Important Economic crop of China, is also grand strategy goods and materials simultaneously.The root of cotton, stem, leaf, seed and cotton fiber all have use value, maximum with the use value of cotton fiber.Cotton fiber is the main production raw material of various wollen fabrics, cotton for wadding and medical cotton.In light industry, the driving-belt of asbestos friction material, automobile and aero tyre also needs to use cotton.At present, due to international environment, crisis of resource, China starts to realize the strategic decision of mutually promoting with coordinating of social development and resource, environment.There is according to mycorrhizal fungi the mineral nutrient in activating soil, promote that root system of plant is to the absorption of the poor phosphorus ore prime element of nutritive element especially mobility, the phosphorus in activating soil; Also promote that host plant is to the absorption of ammonium ion and transport, strengthens the advantage of the capture ability of organic decomposition and nitrogen.Therefore, mycorrhizal fungi is described as " bio-fertilizer ".According to principle and the thinking of the ecological balance and sustainable development, biological technology of mycorrhiza is produced in especially Cotton Production economic development significant at promotion agriculture forest and husbandry.Biological technology of mycorrhiza at the anti-evil of sick worm, high yield, excellent product, and having broad application prospects in China's development of the West Regions process.
Domestic at present, to the research of the syntaxial system between arbuscular mycorrhizal fungi and cotton, mainly concentrate AM fungi to the research field of cotton verticillium wilt disease resistance, but, the disease resistance of research AM fungi and cotton, first will determine the size infecting gesture of AM fungi and cotton.At present, to the application of arbuscular mycorrhizal fungi kind mainly concentrate on Glomus mosseae (
glomus mosseae), Glomus fasciculatum (
glomus fasiculatum) and pearl macrocyst mould (
gigaspora margarita), and to Glomus intraradices (
glomus intraradices) application study less, Glomus intraradices can be described as a quasi-mode fungi of arbuscular mycorrhiza.Research Glomus intraradices and cotton infect relation, be follow-up disease resistance, the basis that of studying such as drought resistance is main.And arbuscular mycorrhizal fungi and plant infect the main impact by soil Middle nutrition material, next is exactly temperature, moisture, illumination etc., simultaneously, the area of the absorption nutrition of the expansion of the outer mycelia of the root due to arbuscular mycorrhizal fungi, can improve the availability of host plant to nutrition, thus reduce the loss of fertilizer.
Summary of the invention
For prior art Problems existing, the object of the invention is to design provides AM fungal infection cotton to improve the application of output of cotton and the technical scheme of method thereof.
Described AM fungal infection cotton improves the method for output of cotton, it is characterized in that comprising the following steps:
1) the cultivation box put into by cotton seeds containing river sand carries out nursery;
2) treat Cotton Seed, when growing 4 leaves, add the arbuscular mycorrhizal fungi liquid bacterial agent of 2-3ml at every strain cotton root, spore amount in microbial inoculum is 200-300/ml, continue to cultivate 4-5 week, applied every 7 days between culture period once to limit nitrogen limit phosphorus nutrition liquid, every day adds water;
3) by step 2) cotton plants that obtains transplants and cultivates to containing in the Culture basin of sand;
4), after cotton plants cultivates 10 days, applied every 7 days once to limit nitrogen limit phosphorus nutrition liquid;
5) cotton plants nursery is after 40 days, transplants cotton plants to experimental field;
6) cotton plants is transplanted to experimental field after 30 days, applies composite fertilizer at cotton plants root, applies water, after this no longer apply fertilizer every 15 days;
7) after 3 months, results cotton.
Described AM fungal infection cotton improves the method for output of cotton, it is characterized in that in described step 1), the front running water of cotton seeds nursery soaks 1 day.
Described AM fungal infection cotton improves the method for output of cotton, it is characterized in that described step 1) and 2) in adopt illumination box cultivate, condition of culture is diurnal temperature is 18/28 DEG C, and humidity is 70%, intensity of illumination is 100%, cultivates 1-2 week.
Described AM fungal infection cotton improves the method for output of cotton, it is characterized in that described limit nitrogen limit phosphorus nutrition liquid contains calcium chloride 180-185mg/l, potassium chloride 60-65mg/l, ammonium nitrate 8000-8020mg/l, potassium dihydrogen phosphate 3-3.4mg/l, anhydrous magnesium sulfate 240-260 mg/l, ferrous sulfate heptahydrate 13.9-14.0 mg/l, EDETATE SODIUM 18.6-19.0 mg/l, boric acid 0.286-0.300 mg/l, manganese chloride 0.181-0.190 mg/l, white vitriol 0.022-0.032 mg/l, cupric sulfate pentahydrate 0.008-0.018 mg/l, sodium molybdate 0.009-0.020 mg/l, N:P=31:11200.
Described AM fungal infection cotton improves the method for output of cotton, it is characterized in that described step 2) in applied limit nitrogen limit phosphorus nutrition liquid 5-10ml/ strain every 7 days between culture period.
Described AM fungal infection cotton improves the method for output of cotton, it is characterized in that applying every 7 days in described step 4) once to limit nitrogen limit phosphorus nutrition liquid 1-5ml/ strain.
Described AM fungal infection cotton improves the method for output of cotton, and it is characterized in that the N:P:K=15:15:15 of described composite fertilizer, the amount of application of composite fertilizer is 5g/ strain.
Described AM fungal infection cotton improves the method for output of cotton, it is characterized in that described arbuscular mycorrhizal fungi be Glomus intraradices bacterium (
glomus intraradices).
Described AM fungal infection cotton improves the application of output of cotton.
Compared with prior art, the invention has the advantages that: the phosphorus content of the nutrient solution 1) applied is very low, and nitrogen content is very high, in agricultural production application, can reduce the use of phosphate fertilizer, thus improve AM fungi and the infection rate of cotton and the output of cotton; 2) simple to operate, to eco-friendly.
Accompanying drawing explanation
Fig. 1 is that Glomus intraradices bacterium and cotton infect figure;
Fig. 2 be cultivation after 30 days Glomus intraradices bacterium situation map is infected to cotton.
Embodiment
The present invention is further illustrated below in conjunction with embodiment.
Embodiment
1) cotton seeds running water is soaked 1 day, then the cotton seeds soaked is put into the cultivation box nursery containing river sand, cultivation box is placed in illumination box and cultivates, condition of culture is diurnal temperature is 18/28 DEG C, humidity is 70%, and intensity of illumination is 100%, cultivates 1-2 week;
2) treat Cotton Seed, when growing 4 leaves, add the arbuscular mycorrhizal fungi liquid bacterial agent of 3ml at every strain cotton root, the spore amount in microbial inoculum is 200-300/ml, continues 4 week of cultivation;
3) in step 2) grown cultures process in, added every 7 days and once limit nitrogen limit phosphorus nutrition liquid 5-10ml/ strain, add the moisture of appropriate amount every day;
4) after 4 week, collect the root of cotton, measure the infection rate of mycorhiza with the blue dyeing-grid interior extrapolation method of the sharp benzene of song;
5) cotton plants simultaneously infected by the AM microbial inoculum cultivated under similarity condition and the cotton plants not having AM microbial inoculum to infect are transplanted and are cultivated to containing in the Culture basin of sand;
6) planting seed is after 10 days, applies once to limit nitrogen limit phosphorus nutrition liquid 1-5ml/ strain to whole cotton plants every 7 days;
7) nursery is after 40 days, and the seedling of 42 strain inoculation AM microbial inoculum cottons and 27 strains are not transplanted to experimental field with the cotton seedling of microbial inoculum, the soil property of experimental field is red soil, and the total nitrogen content wherein recorded in soil is 1.51g/kg, and available phosphorus content is 1.09g/kg;
8) transplant to experimental field after 30 days, apply composite fertilizer 5g/ strain at cotton root, applied amount of water every 7 days, after this no longer apply fertilizer, wherein the weight ratio of composite fertilizer N:P:K is 15:15:15;
9) after 3 months, results cotton, measurement data.
Above-mentioned limit nitrogen limit phosphorus nutrition liquid contains calcium chloride 182mg/l, potassium chloride 62mg/l, ammonium nitrate 8000mg/l, potassium dihydrogen phosphate 3.2mg/l, anhydrous magnesium sulfate 250 mg/l, ferrous sulfate heptahydrate 14.0 mg/l, EDETATE SODIUM 18.8 mg/l, boric acid 0.29mg/l, manganese chloride 0.185 mg/l, white vitriol 0.026mg/l, cupric sulfate pentahydrate 0.010mg/l, sodium molybdate 0.015mg/l, N:P=31:11200.Above-mentioned arbuscular mycorrhizal fungi be Glomus intraradices bacterium (
glomus intraradices).
As can be seen from Fig. 1 and 2, after adding Glomus intraradices microbial inoculum, cultivate after 30 days, the infection rate of the cotton mycorhiza recorded reaches as high as 95%, and minimum 67%, by the distributed points on chart, find out that the infection rate of cotton and AM bacterium is at 60%-95%.
The individual plant cotton boll number applying AM microbial inoculum as can be seen from Table 1 improves 6.5% than the individual plant cotton cotton boll number not adding AM microbial inoculum, meanwhile, the cotton boll gross yield of cotton, the fresh weight of individual plant product cotton boll and total cotton boll fresh weight significantly improve 13.71%, 16.74% and 16.06% than the cotton not adding bacterium; For the output of individual plant cotton, find out from table 1, individual plant with the cotton of microbial inoculum produces the gross yield of cotton amount and cotton all higher than the cotton plants not adding bacterium, and all improve 12.94%, this illustrates that the root system interpolation AM microbial inoculum in cotton can improve the output of cotton, plays a positive role to the productivity effect of cotton.Draw from table 2, the average plant height applying the cotton of microbial inoculum improves 6.59% than the cotton plant height with microbial inoculum, simultaneously, the fresh weight and the total fresh weight of cotton field upper part that apply the individual plant cotton of AM microbial inoculum are better than the single-strain fresh weight of the cotton not adding bacterium and total fresh weight, improve 44.84% and 10.83% respectively, but from the data representation that table 2 draws, the total nitrogen content of the cauline leaf of cotton, the cotton applying microbial inoculum reduces 2.23% and 7.33% than not respectively with the total nitrogen content of the cotton of microbial inoculum, in addition, the total phosphorus content adding the leaf of the cotton of bacterium improves 13.31% than the total phosphorus content not adding bacterium, but the total phosphorus content in stem but reduces 58.06% than the cotton not adding bacterium, these experimental datas show, the plant height and the acrial part fresh weight that apply the cotton of AM microbial inoculum are obviously better than the cotton not adding AM fungi, but in total nitrogen content and leaf, total phosphorus content is starkly lower than not with the cotton of microbial inoculum in cauline leaf, then illustrate that the cotton being applied with microbial inoculum is unfavorable for the accumulation of total phosphorus in total nitrogen and stem in cauline leaf, it is relevant for its growth and breeding that this and host need to supply some nutriments of AM fungi.
The present invention limits phosphorus nutrition liquid for nutriment to limit nitrogen, the infection rate of effective raising AM fungi and cotton, the cotton of sowing in soil extends the outer mycelia of a large amount of roots by efficient infection rate, enlarged-area absorbs the nitrogen and phosphorus element in soil, thus improve the output of cotton, reduce the waste of fertilizer.
table 1 output of cotton measures
note: data processing adopts the mean value of individual plant output of cotton to be multiplied by the gross yield that 27 strain cottons try to achieve cotton
table 2 cotton biomass estimation
Claims (9)
1.AM fungal infection cotton improves the method for output of cotton, it is characterized in that comprising the following steps:
1) the cultivation box put into by cotton seeds containing river sand carries out nursery;
2) treat Cotton Seed, when growing 4 leaves, add the arbuscular mycorrhizal fungi liquid bacterial agent of 2-3ml at every strain cotton root, spore amount in microbial inoculum is 200-300/ml, continue to cultivate 4-5 week, applied every 7 days between culture period once to limit nitrogen limit phosphorus nutrition liquid, every day adds water;
3) by step 2) cotton plants that obtains transplants and cultivates to containing in the Culture basin of sand;
4), after cotton plants cultivates 10 days, applied every 3 days once to limit nitrogen limit phosphorus nutrition liquid;
5) cotton plants nursery is after 40 days, transplants cotton plants to experimental field;
6) cotton plants is transplanted to experimental field after 30 days, applies composite fertilizer at cotton plants root, applies water, after this no longer apply fertilizer every 15 days;
7) after 3 months, results cotton.
2. AM fungal infection cotton as claimed in claim 1 improves the method for output of cotton, it is characterized in that in described step 1), the front running water of cotton seeds nursery soaks 1 day.
3. AM fungal infection cotton as claimed in claim 1 improves the method for output of cotton, it is characterized in that described step 1) and 2) in adopt illumination box cultivate, condition of culture is diurnal temperature is 18/28 DEG C, and humidity is 70%, intensity of illumination is 100%, cultivates 4-5 week.
4. AM fungal infection cotton as claimed in claim 1 improves the method for output of cotton, it is characterized in that described limit nitrogen limit phosphorus nutrition liquid contains calcium chloride 180-185mg/l, potassium chloride 60-65mg/l, ammonium nitrate 8000-8020mg/l, potassium dihydrogen phosphate 3-3.4mg/l, anhydrous magnesium sulfate 240-260 mg/l, ferrous sulfate heptahydrate 13.9-14.0 mg/l, EDETATE SODIUM 18.6-19.0 mg/l, boric acid 0.286-0.300 mg/l, manganese chloride 0.181-0.190 mg/l, white vitriol 0.022-0.032 mg/l, cupric sulfate pentahydrate 0.008-0.018 mg/l, sodium molybdate 0.009-0.020 mg/l, N:P=31:11200.
5. AM fungal infection cotton as claimed in claim 1 improves the method for output of cotton, it is characterized in that described step 2) in applied limit nitrogen limit phosphorus nutrition liquid 5-10ml/ strain every 7 days between culture period.
6. AM fungal infection cotton as claimed in claim 1 improves the method for output of cotton, it is characterized in that applying every 7 days in described step 4) once to limit nitrogen limit phosphorus nutrition liquid 1-5ml/ strain.
7. AM fungal infection cotton as claimed in claim 1 improves the method for output of cotton, and it is characterized in that described composite fertilizer N:P:K=15:15:15, the amount of application of composite fertilizer is 5g/ strain.
8. AM fungal infection cotton as claimed in claim 1 improves the method for output of cotton, it is characterized in that described arbuscular mycorrhizal fungi be Glomus intraradices bacterium (
glomus intraradices).
9.AM fungal infection cotton improves the application of output of cotton.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104928191A (en) * | 2015-06-25 | 2015-09-23 | 无锡本元生物科技有限公司 | Arbuscular mycorrhizal fungi GZ176 for disease prevention, growth promotion and adverse resistance of herbaceous plants and microbial inoculums and application of arbuscular mycorrhizal fungi GZ176 |
| CN106508429A (en) * | 2016-11-10 | 2017-03-22 | 中国矿业大学(北京) | Multiplication method of arbuscular mycorrhiza fungi inoculant in coal mine area |
| CN109906893A (en) * | 2019-03-18 | 2019-06-21 | 浙江师范大学 | A kind of method that Mycorrhizal higher yield of crops in farmland is high-quality |
-
2015
- 2015-01-26 CN CN201510037602.9A patent/CN104604557A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104928191A (en) * | 2015-06-25 | 2015-09-23 | 无锡本元生物科技有限公司 | Arbuscular mycorrhizal fungi GZ176 for disease prevention, growth promotion and adverse resistance of herbaceous plants and microbial inoculums and application of arbuscular mycorrhizal fungi GZ176 |
| CN104928191B (en) * | 2015-06-25 | 2019-04-26 | 南京本源生态农业科技有限公司 | For herbaceous plant diseases prevention, growth-promoting, degeneration-resistant arbuscular mycorrhizal fungi GZ176 and its microbial inoculum and application |
| CN106508429A (en) * | 2016-11-10 | 2017-03-22 | 中国矿业大学(北京) | Multiplication method of arbuscular mycorrhiza fungi inoculant in coal mine area |
| CN109906893A (en) * | 2019-03-18 | 2019-06-21 | 浙江师范大学 | A kind of method that Mycorrhizal higher yield of crops in farmland is high-quality |
| CN109906893B (en) * | 2019-03-18 | 2022-02-01 | 浙江师范大学 | Method for high yield and high quality of farmland mycorrhizal crops |
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