CN109182204A - A kind of microbe leaven and preparation method thereof - Google Patents
A kind of microbe leaven and preparation method thereof Download PDFInfo
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- CN109182204A CN109182204A CN201811172022.0A CN201811172022A CN109182204A CN 109182204 A CN109182204 A CN 109182204A CN 201811172022 A CN201811172022 A CN 201811172022A CN 109182204 A CN109182204 A CN 109182204A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The present invention relates to microorganisms technical fields, more particularly to a kind of microbe leaven and preparation method thereof, which is made of following parts by weight raw material: 20-30 parts of bacillus subtilis, 10-15 parts of bacillus coagulans, 15-20 parts of bacillus megaterium, 15-20 parts of bacillusmusilaginosiengineering, 6-9 parts of aspergillus oryzae, 10-17 parts of bacillus licheniformis, 5-10 parts of Paecilomyces lilacinus, 7-12 parts of Streptomyces jingyangensis；Technical solution provided by the invention effectively compensates for be had a single function existing for existing microbe leaven, the defect for promoting Effects on Plant Growth weak.
Description
Technical field
The present invention relates to microorganisms technical fields, and in particular to a kind of microbe leaven and preparation method thereof.
Background technique
Microorganism includes: one big including bacterium, virus, fungi and some small-sized protists, microalga etc.
Class biocenose, its individual is small, close with human relation.Cover beneficial to harmful numerous kinds, relate generally to food,
The numerous areas such as medicine, industrial or agricultural, environmental protection.In the textbook of CONTINENTAL AREA OF CHINA, it is big that microorganism is divided into following 8
Class: bacterium, virus, fungi, actinomyces, Richettsia, mycoplasma, Chlamydia, conveyor screw.Some microorganisms are that naked eyes can be seen
See, as mushroom, the ganoderma lucidum etc. for belonging to fungi.There are also microorganism be it is a kind of by nucleic acid and protein etc. a few at being grouped as
" acellular organism ", but its existence is necessarily dependent upon living cells.
The raw ferment agent of microorganism is a kind of multifunction additive new product, has concentrated effective microorganisms, probiotic, enzyme system
Agent, leavening, deodorant, animal health-care product, growth promoter, hypnotic, the comprehensive function for promoting fragrant and sweet dose of food, Chinese herb feed additive,
And the long factor, magic power agent to promote food and Chinese herbal medicines preparation are urged containing potent, vigor is superpower, and cost is only same currently on the market
Less than half of class product product.The product after use, has obtained higher praise throughout the country.
Chinese patent CN103834604B discloses a kind of multi-cultur es microbe leaven and preparation method thereof at present.Pass through
First by bacillus subtilis, bacillus megaterium, black-koji mould, streptococcus lactis, production gastral cavity Candida, Corynebacterium glutamicum point
Not carry out actication of culture, inclined-plane culture, after by above-mentioned 6 kinds of microorganism fungus kinds by number percent proportion mixing be put into triangular flask
In, it is placed in shaking table, is cultivated 2-3 days under conditions of 28 DEG C, control pH makes bacterium number content up to hundred million/ml of 6-8, system in 6.5-7.0
Obtain the multi-cultur es microbe leaven.The selected specific 6 kinds of microbial bacterias of navel orange feature are directed to by using the present invention
Multi-cultur es microbe leaven prepared by kind, can improve the quality of navel orange, improve the yield of navel orange, and yield is made to increase production 10-
15%, to meet the needs of price peak period.
But existing microbe leaven, which exists, to be had a single function, the defect for promoting Effects on Plant Growth weak.
Summary of the invention
In order to overcome the shortcomings of the prior art, the object of the present invention is to provide a kind of microbe leaven and its preparations
Method is effectively compensated for and is had a single function existing for existing microbe leaven, the defect for promoting Effects on Plant Growth weak.
In order to achieve the above purpose, it is achieved by the following technical programs:
A kind of microbe leaven, the microbe leaven are made of following parts by weight raw material: bacillus subtilis 20-
30 parts, 10-15 parts of bacillus coagulans, 15-20 parts of bacillus megaterium, 15-20 parts of bacillusmusilaginosiengineering, aspergillus oryzae 6-9
Part, 10-17 parts of bacillus licheniformis, 5-10 parts of Paecilomyces lilacinus, 7-12 parts of Streptomyces jingyangensis.
Preferably, which is made of following parts by weight raw material: 22-25 parts of bacillus subtilis, condensation
11-13 parts of bacillus, 16-19 parts of bacillus megaterium, 17-18 parts of bacillusmusilaginosiengineering, 7-8 parts of aspergillus oryzae, lichens bud
12-15 parts of spore bacillus, 6-8 parts of Paecilomyces lilacinus, 8-10 parts of Streptomyces jingyangensis.
A kind of preparation method of microbe leaven, comprises the following steps:
1) peeled potatoes 200-210g is taken, fritter is cut, adds water 900-950ml, boils 30min, filters off potato, is added
Glucose 25-30g, agar 10-15g, moisturizing to, heating for dissolving equal with initial amount of water, 15 pounds of sterilizings a period of times；
2) step 1) resulting material is added in triangular flask, bacillus subtilis, bacillus coagulans, huge gemma is added
Bacillus, bacillusmusilaginosiengineering, aspergillus oryzae, bacillus licheniformis, Paecilomyces lilacinus, Streptomyces jingyangensis, triangular flask is placed in and is shaken
In bed, control pH is certain, 4-5 days is cultivated under the conditions of certain temperature to obtain the final product.
Preferably, sterilization time is 25-30min in the step 1).
Preferably, pH is 6.5-7.0 in the step 2).
Preferably, cultivation temperature is 25-27 DEG C in the step 2).
Using above-mentioned technical solution, beneficial effects of the present invention are as follows:
Bacillus subtilis, bacillus coagulans are used cooperatively, and can be reduced nitrate content and heavy metal in plant and be contained
Amount makes Vc content in fruit improve 30% or more.
Bacillus megaterium, bacillusmusilaginosiengineering are used cooperatively, and generate a large amount of plant endogenous enzyme in the metabolic process,
Crop is significantly improved to the absorptivity of the nutrients such as nitrogen, phosphorus, potassium.
Bacillusmusilaginosiengineering and bacillus licheniformis cooperate, and can promote crop root growth, and fibrous root increases, beneficial to micro- life
The plant endogenous enzyme and plant growth regulator that object bacterial metabolism generates enter in plant through root system, promote photosynthesis, adjust
It saves nutrient to flow toward fruit, swollen fruit effect of increasing production is obvious.
Paecilomyces lilacinus has prevention and treatment function to a variety of nematodes, and Streptomyces jingyangensis has enhancing soil fertility, stimulation crop
The ability of growth.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention,
Technical scheme in the embodiment of the invention is clearly and completely described.Based on the embodiment of the present invention, the common skill in this field
Art personnel every other embodiment obtained without creative efforts belongs to the model that the present invention protects
It encloses.
Embodiment 1:
A kind of microbe leaven, the microbe leaven are made of following parts by weight raw material: bacillus subtilis 20
Part, 15 parts of bacillus coagulans, 15 parts of bacillus megaterium, 20 parts of bacillusmusilaginosiengineering, 6 parts of aspergillus oryzae, lichens gemma bar
17 parts of bacterium, 5 parts of Paecilomyces lilacinus, 12 parts of Streptomyces jingyangensis.
A kind of microbe leaven and preparation method thereof, comprises the following steps:
1) peeled potatoes 200g is taken, fritter is cut, adds water 950ml, boils 30min, filters off potato, glucose is added
25g, agar 15g, moisturizing to, heating for dissolving equal with initial amount of water, 15 pounds of sterilizing 25min；
2) step 1) resulting material is added in triangular flask, bacillus subtilis, bacillus coagulans, huge gemma is added
Bacillus, bacillusmusilaginosiengineering, aspergillus oryzae, bacillus licheniformis, Paecilomyces lilacinus, Streptomyces jingyangensis, triangular flask is placed in and is shaken
In bed, control pH is 7.0,5 days is cultivated under the conditions of 25 DEG C to obtain the final product.
Embodiment 2:
A kind of microbe leaven, the microbe leaven are made of following parts by weight raw material: bacillus subtilis 30
Part, 10 parts of bacillus coagulans, 20 parts of bacillus megaterium, 15 parts of bacillusmusilaginosiengineering, 9 parts of aspergillus oryzae, lichens gemma bar
10 parts of bacterium, 10 parts of Paecilomyces lilacinus, 7 parts of Streptomyces jingyangensis.
A kind of microbe leaven and preparation method thereof, comprises the following steps:
1) peeled potatoes 210g is taken, fritter is cut, adds water 900ml, boils 30min, filters off potato, glucose is added
30g, agar 10g, moisturizing to, heating for dissolving equal with initial amount of water, 15 pounds of sterilizing 30min；
2) step 1) resulting material is added in triangular flask, bacillus subtilis, bacillus coagulans, huge gemma is added
Bacillus, bacillusmusilaginosiengineering, aspergillus oryzae, bacillus licheniformis, Paecilomyces lilacinus, Streptomyces jingyangensis, triangular flask is placed in and is shaken
In bed, control pH is 6.5,4 days is cultivated under the conditions of 27 DEG C to obtain the final product.
Embodiment 3:
A kind of microbe leaven, the microbe leaven are made of following parts by weight raw material: bacillus subtilis 22
Part, 13 parts of bacillus coagulans, 16 parts of bacillus megaterium, 18 parts of bacillusmusilaginosiengineering, 7 parts of aspergillus oryzae, lichens gemma bar
15 parts of bacterium, 6 parts of Paecilomyces lilacinus, 10 parts of Streptomyces jingyangensis.
A kind of microbe leaven and preparation method thereof, comprises the following steps:
1) peeled potatoes 200g is taken, fritter is cut, adds water 910ml, boils 30min, filters off potato, glucose is added
25g, agar 10g, moisturizing to, heating for dissolving equal with initial amount of water, 15 pounds of sterilizing 30min；
2) step 1) resulting material is added in triangular flask, bacillus subtilis, bacillus coagulans, huge gemma is added
Bacillus, bacillusmusilaginosiengineering, aspergillus oryzae, bacillus licheniformis, Paecilomyces lilacinus, Streptomyces jingyangensis, triangular flask is placed in and is shaken
In bed, control pH is 7.0,4 days is cultivated under the conditions of 25 DEG C to obtain the final product.
Embodiment 4:
A kind of microbe leaven, the microbe leaven are made of following parts by weight raw material: bacillus subtilis 25
Part, 11 parts of bacillus coagulans, 19 parts of bacillus megaterium, 17 parts of bacillusmusilaginosiengineering, 8 parts of aspergillus oryzae, lichens gemma bar
12 parts of bacterium, 8 parts of Paecilomyces lilacinus, 8 parts of Streptomyces jingyangensis.
A kind of microbe leaven and preparation method thereof, comprises the following steps:
1) peeled potatoes 210g is taken, fritter is cut, adds water 920ml, boils 30min, filters off potato, glucose is added
30g, agar 10g, moisturizing to, heating for dissolving equal with initial amount of water, 15 pounds of sterilizing 25min；
2) step 1) resulting material is added in triangular flask, bacillus subtilis, bacillus coagulans, huge gemma is added
Bacillus, bacillusmusilaginosiengineering, aspergillus oryzae, bacillus licheniformis, Paecilomyces lilacinus, Streptomyces jingyangensis, triangular flask is placed in and is shaken
In bed, control pH is 7.0,4 days is cultivated under the conditions of 25 DEG C to obtain the final product.
Embodiment 5:
A kind of microbe leaven, the microbe leaven are made of following parts by weight raw material: bacillus subtilis 24
Part, 12 parts of bacillus coagulans, 17 parts of bacillus megaterium, 17 parts of bacillusmusilaginosiengineering, 8 parts of aspergillus oryzae, lichens gemma bar
13 parts of bacterium, 7 parts of Paecilomyces lilacinus, 9 parts of Streptomyces jingyangensis.
A kind of microbe leaven and preparation method thereof, comprises the following steps:
1) peeled potatoes 200g is taken, fritter is cut, adds water 930ml, boils 30min, filters off potato, glucose is added
30g, agar 15g, moisturizing to, heating for dissolving equal with initial amount of water, 15 pounds of sterilizing 25min；
2) step 1) resulting material is added in triangular flask, bacillus subtilis, bacillus coagulans, huge gemma is added
Bacillus, bacillusmusilaginosiengineering, aspergillus oryzae, bacillus licheniformis, Paecilomyces lilacinus, Streptomyces jingyangensis, triangular flask is placed in and is shaken
In bed, control pH is 6.5,5 days is cultivated under the conditions of 27 DEG C to obtain the final product.
Multi-cultur es microbe leaven made from embodiment 1-5 and normal fermentation agent are compared into performance test, as a result
It is as follows:
| Navel orange (kilogram) | Fermentation time (hour) | Fermentation yield (kilogram) | |
| Embodiment 1 | 1000 | 96 | 630 |
| Embodiment 2 | 1000 | 96 | 620 |
| Embodiment 3 | 1000 | 96 | 615 |
| Embodiment 4 | 1000 | 96 | 625 |
| Embodiment 5 | 1000 | 96 | 630 |
| Normal fermentation agent | 1000 | 144 | 460 |
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although with reference to the foregoing embodiments
Invention is explained in detail, those skilled in the art should understand that: it still can be to aforementioned each implementation
Technical solution documented by example is modified or equivalent replacement of some of the technical features；And these modification or
Replacement, the spirit and scope for technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution.
Claims (6)
1. a kind of microbe leaven, which is characterized in that the microbe leaven is made of following parts by weight raw material: withered grass bud
20-30 parts of spore bacillus, 10-15 parts of bacillus coagulans, 15-20 parts of bacillus megaterium, 15-20 parts of bacillusmusilaginosiengineering,
6-9 parts of aspergillus oryzae, 10-17 parts of bacillus licheniformis, 5-10 parts of Paecilomyces lilacinus, 7-12 parts of Streptomyces jingyangensis.
2. microbe leaven according to claim 1, which is characterized in that the microbe leaven is by following parts by weight
Raw material is made: 22-25 parts of bacillus subtilis, 11-13 parts of bacillus coagulans, 16-19 parts of bacillus megaterium, gel-shaped bud
17-18 parts of spore bacillus, 7-8 parts of aspergillus oryzae, 12-15 parts of bacillus licheniformis, 6-8 parts of Paecilomyces lilacinus, Streptomyces jingyangensis 8-10
Part.
3. a kind of preparation method of -2 any microbe leaven according to claim 1, which is characterized in that including such as
Under several steps:
1) peeled potatoes 200-210g is taken, fritter is cut, adds water 900-950ml, boils 30min, filters off potato, grape is added
Sugared 25-30g, agar 10-15g, moisturizing to, heating for dissolving equal with initial amount of water, 15 pounds of sterilizings a period of times；
2) step 1) resulting material is added in triangular flask, bacillus subtilis, bacillus coagulans, huge gemma bar is added
Triangular flask is placed in shaking table by bacterium, bacillusmusilaginosiengineering, aspergillus oryzae, bacillus licheniformis, Paecilomyces lilacinus, Streptomyces jingyangensis
Interior, control pH is certain, 4-5 days is cultivated under the conditions of certain temperature to obtain the final product.
4. the preparation method of microbe leaven according to claim 3, which is characterized in that in the step 1) when sterilizing
Between be 25-30min.
5. the preparation method of microbe leaven according to claim 3, which is characterized in that pH is in the step 2)
6.5-7.0。
6. the preparation method of microbe leaven according to claim 3, which is characterized in that culture temperature in the step 2)
Degree is 25-27 DEG C.
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| CN201811172022.0A CN109182204A (en) | 2018-10-09 | 2018-10-09 | A kind of microbe leaven and preparation method thereof |
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| CN201811172022.0A CN109182204A (en) | 2018-10-09 | 2018-10-09 | A kind of microbe leaven and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110156515A (en) * | 2019-05-31 | 2019-08-23 | 云南联展科技有限公司 | A kind of biological material and its preparation process using organic microbial fermentation |
| CN111825506A (en) * | 2020-07-30 | 2020-10-27 | 上海绿乐生物科技有限公司 | Special fertilizer for improving ecological environment of rice soil and application method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110156515A (en) * | 2019-05-31 | 2019-08-23 | 云南联展科技有限公司 | A kind of biological material and its preparation process using organic microbial fermentation |
| CN111825506A (en) * | 2020-07-30 | 2020-10-27 | 上海绿乐生物科技有限公司 | Special fertilizer for improving ecological environment of rice soil and application method thereof |
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Application publication date: 20190111 |