CN109294941A - A kind of microbial compound bacterial fertilizer and the application in promotion plant absorption N P and K - Google Patents
A kind of microbial compound bacterial fertilizer and the application in promotion plant absorption N P and K Download PDFInfo
- Publication number
- CN109294941A CN109294941A CN201811048010.7A CN201811048010A CN109294941A CN 109294941 A CN109294941 A CN 109294941A CN 201811048010 A CN201811048010 A CN 201811048010A CN 109294941 A CN109294941 A CN 109294941A
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- CN
- China
- Prior art keywords
- bacillus
- water diluent
- microbial compound
- bacterial fertilizer
- water
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/45—Tobacco
Abstract
The invention discloses a kind of microbial compound bacterial fertilizer and promoting the application in plant absorption N P and K, the bacterial manure is made of following methods: (1) weighing bacillus megaterium, Bacillus subtillis and bacillus amyloliquefaciens bacterial manure respectively, the distilled water dilution of 200 mass times is respectively added, respectively obtain bacillus megaterium water diluent, Bacillus subtillis water diluent and bacillus amyloliquefaciens water diluent；(2) by volume it is 1:(1-3): the ratio of (1-3), by bacillus megaterium water diluent, Bacillus subtillis water diluent and bacillus amyloliquefaciens water diluent mix to obtain microbial compound bacterial fertilizer.Method of the invention is efficiently simple and direct, inexpensive, not only ensure that soil fertility, but also improves plant to the uptake of N P and K, substitutes chemical fertilizer and uses, also can avoid pollution on the environment.
Description
Technical field
The invention belongs to microbial-bacterial fertilizer applied technical field, it is related to a kind of microbial compound bacterial fertilizer and promotes plant absorption
Application method in N P and K.
Background technique
A large amount of applications of China's agriculture chemistry fertilizer not only cause soil hardening, soil microflora to change, and
And food-safety problem is brought, also cause a large amount of economic loss and serious environmental pollution；A large amount of applications of chemical pesticide, are controlled
Mark does not effect a permanent cure, and disease pest has produced drug resistance, and the residual hazard of fertilizer and pesticide severely compromises the health of the mankind.And bio-bacterial manure is exactly
It can solve these problems, it carrys out activating soil by the procreation of beneficial bacterium, supplies various nutrition required for crop, has fertilizing ground
Power, drought resisting, promotees precocious effect at improvement soil, moreover it is possible to solve the problems, such as Pesticide-Polluted Soil, be that production green food is indispensable
Fertilizer.
Under current agricultural sustainable development situation, it is paid more and more attention using microbial manure part replacing fertilizer.And
And a kind of safety and environmental protection is needed at present, plant not only can be improved to the absorbability of N P and K, but also mass production is suitble to use
Microbial compound bacterial fertilizer.
Summary of the invention
The purpose of the present invention is overcome the deficiencies of the prior art and provide a kind of microbial compound bacterial fertilizer.
A second object of the present invention is to provide a kind of microbial compound bacterial fertilizers to promote answering in plant absorption N P and K
With.
Technical scheme is as follows:
A kind of microbial compound bacterial fertilizer, is made of following methods:
(1) bacillus megaterium, Bacillus subtillis and bacillus amyloliquefaciens bacterial manure are weighed respectively, are respectively added 200
The distilled water dilution of quality times, respectively obtains bacillus megaterium water diluent, and Bacillus subtillis water diluent conciliates starch
Bacillus water diluent；
(2) by volume it is 1:(1-3): the ratio of (1-3), by bacillus megaterium water diluent, Bacillus subtillis
Water diluent and bacillus amyloliquefaciens water diluent mix to obtain microbial compound bacterial fertilizer.
A kind of above-mentioned microbial compound bacterial fertilizer is promoting the application in plant absorption N P and K.
Preferably, by mentioned microorganism composite bacterial fertilizer according to 35-45ml/m2Dosage be applied to depth of soil 5-10cm
Place；By 24-26 hours, planting plants seedling.
The preferred corn of the plant, Chinese rose or tobacco, can also select other plants, such as wheat, sorghum.
Advantages of the present invention:
Method of the invention is efficiently simple and direct, inexpensive, not only ensure that soil fertility, but also improve suction of the plant to N P and K
Receipts amount, substitution chemical fertilizer use, and also can avoid pollution on the environment.
Specific embodiment
Bacillus megaterium source: from Shandong, Lv Long Bioisystech Co., Ltd is purchased.
Bacillus subtillis source: from Shandong, Lv Long Bioisystech Co., Ltd is purchased.
Bacillus amyloliquefaciens source: from Shandong, Lv Long Bioisystech Co., Ltd is purchased.
The present invention is further illustrated combined with specific embodiments below.
Embodiment 1
A kind of microbial compound bacterial fertilizer, is made of following methods:
(1) bacillus megaterium, Bacillus subtillis and bacillus amyloliquefaciens bacterial manure are weighed respectively, are respectively added 200
The distilled water dilution of quality times, respectively obtains bacillus megaterium water diluent, and Bacillus subtillis water diluent conciliates starch
Bacillus water diluent；
It (2) is by volume the ratio of 1:2:2, by bacillus megaterium water diluent, Bacillus subtillis water diluent
Microbial compound bacterial fertilizer is mixed to obtain with bacillus amyloliquefaciens water diluent.
Embodiment 2
A kind of microbial compound bacterial fertilizer, is made of following methods:
(1) bacillus megaterium, Bacillus subtillis and bacillus amyloliquefaciens bacterial manure are weighed respectively, are respectively added 200
The distilled water dilution of quality times, respectively obtains bacillus megaterium water diluent, and Bacillus subtillis water diluent conciliates starch
Bacillus water diluent；
It (2) is by volume the ratio of 1:1:3, by bacillus megaterium water diluent, Bacillus subtillis water diluent
Microbial compound bacterial fertilizer is mixed to obtain with bacillus amyloliquefaciens water diluent.
Embodiment 3
A kind of microbial compound bacterial fertilizer, is made of following methods:
(1) bacillus megaterium, Bacillus subtillis and bacillus amyloliquefaciens bacterial manure are weighed respectively, are respectively added 200
The distilled water dilution of quality times, respectively obtains bacillus megaterium water diluent, and Bacillus subtillis water diluent conciliates starch
Bacillus water diluent；
It (2) is by volume the ratio of 1:3:1, by bacillus megaterium water diluent, Bacillus subtillis water diluent
Microbial compound bacterial fertilizer is mixed to obtain with bacillus amyloliquefaciens water diluent.
Embodiment 4
A kind of microbial compound bacterial fertilizer is promoting the application in plant absorption N P and K, includes the following steps:
Microbial compound bacterial fertilizer prepared by embodiment 1 is according to 40ml/m2Dosage be applied at depth of soil 8cm (tool
Gymnastics is made: soil topsoil being dug 8cm depth, by microbial compound bacterial fertilizer according to 40ml/m2Dosage uniformly spill, then by surface layer
Soil covering)；By 25 hours, maize planting seedling.
Embodiment 5
A kind of microbial compound bacterial fertilizer is promoting the application in plant absorption N P and K, includes the following steps:
Microbial compound bacterial fertilizer prepared by embodiment 2 is according to 35ml/m2Dosage be applied at depth of soil 5cm；Through
24 hours are spent, tobacco seedling is planted.
Embodiment 6
A kind of microbial compound bacterial fertilizer is promoting the application in plant absorption N P and K, includes the following steps:
Microbial compound bacterial fertilizer prepared by embodiment 3 is according to 45ml/m2Dosage be applied at depth of soil 10cm；Through
26 hours are spent, Chinese rose seedling is planted.
Using same method, it can be used for other plants such as with microbial compound bacterial fertilizer prepared by embodiment 1,2 or 3
Wheat, sorghum seedling.
Embodiment 7
N P and K situation in seedling, specific method and detection knot are detected respectively to plant seedling growth mature (about several days)
Fruit is as follows:
1. the quick measurement of nitrogen (N) element in plant:
1.0g diphenylamines is dissolved in the 100mL concentrated sulfuric acid, diphenylamines sulphate reagent is obtained；
Each plant children stem 0.1g of embodiment 4,5,6 is weighed respectively and is thinly sliced, is put into small beaker, and 2 drop hexichol are instilled
Amine sulphate reagent.As a result all it is in navy blue, indicates that nitrogen quantity is sufficient.
Blue is deeper, indicates that nitrogen content is higher.
2. the quick measurement of phosphorus (P) element in plant:
Each plant children stem 0.1g of embodiment 4,5,6 is weighed respectively to thinly slice, and is put into test tube.1mL reagent a is added (will
63ml concentrated hydrochloric acid is added in 437ml distilled water, then weighs 4g ammonium molybdate, is dissolved in wherein), with forced oscillation 1min, then it is added few
Perhaps dry powdery stannous chloride, which is sufficiently mixed, is allowed to react completely.As a result all it is in navy blue, indicates that phosphorus amount is abundant.
Blue is deeper, indicates that phosphorus content is higher.
3. the quick measurement of potassium (K) element in plant:
Each plant children stem 0.1g of embodiment 4,5,6 is weighed respectively to thinly slice, is put into test tube, and 1ml reagent c is added (first
5g natrium cobaltinitrosum and 30g sodium nitrite are dissolved in distilled water, 5ml glacial acetic acid is added, is finally settled to distilled water
100ml is stood, then the sodium nitrite solution of 5ml15% is taken to be added in above-mentioned mixed liquor, is finally with glacial acetic acid adjustment pH value
5.0), with forced oscillation 1min, it is slow added into 0.5ml95% alcohol.It is observed after static 3min, solution is all muddy, illustrates plant
Potassium content is high.
Solution is all muddy, illustrates that potassium content is higher.
Reference examples
A kind of microbial compound bacterial fertilizer is promoting the application method in plant absorption N P and K, includes the following steps:
1. taking soil maize planting, Chinese rose and the tobacco seedling for not applying microbial compound bacterial fertilizer of the present invention；
2. detecting N P and K situation in seedling respectively after seedling is mature, specific method and testing result are as follows:
1. the quick measurement of nitrogen (N) element in plant:
1.0g diphenylamines is dissolved in the 100ml concentrated sulfuric acid, each plant children stem 0.1g is weighed and is thinly sliced, small beaker is put into
In, instill 2 drop diphenylamines sulphate reagents.As a result micro- indigo plant is all only presented in three samples, indicates that nitrogen content is slightly insufficient.
2. the quick measurement of phosphorus (P) element in plant:
It weighs each plant children stem 0.1g to thinly slice, be put into test tube.1ml reagent a is added (to add 63ml concentrated hydrochloric acid
In 437ml distilled water, then 4g ammonium molybdate is weighed, is dissolved in wherein), with forced oscillation 1min, powdery chlorine dry a little is then added
Change stannous, which is sufficiently mixed, to be allowed to react completely.As a result three samples are all close to colourless, expression phosphorus content deficiency.
3. the quick measurement of potassium (K) element in plant:
Weigh each plant children stem 0.1g to thinly slice, be put into test tube, be added 1ml reagent c (first by 5g natrium cobaltinitrosum and
30g sodium nitrite is dissolved in distilled water, and 5ml glacial acetic acid is added, is finally settled to 100ml with distilled water, is stood, then take
The sodium nitrite solution of 5ml15% is added in above-mentioned mixed liquor, is finally 5.0), to use forced oscillation with glacial acetic acid adjustment pH value
1min is slow added into 0.5ml95% alcohol.It is observed after static 3min, as a result three sample solutions are almost clarified, and illustrate plant
Potassium content is insufficient.
Claims (4)
1. a kind of microbial compound bacterial fertilizer, it is characterized in that being made of following methods:
(1) bacillus megaterium, Bacillus subtillis and bacillus amyloliquefaciens bacterial manure are weighed respectively, and 200 mass are respectively added
Distilled water dilution again, respectively obtains bacillus megaterium water diluent, and Bacillus subtillis water diluent conciliates starch gemma
Bacillus water diluent；
(2) by volume it is 1:(1-3): the ratio of (1-3), by bacillus megaterium water diluent, Bacillus subtillis water is dilute
It releases liquid and bacillus amyloliquefaciens water diluent mixes to obtain microbial compound bacterial fertilizer.
2. a kind of microbial compound bacterial fertilizer of claim 1 is promoting the application in plant absorption N P and K.
3. application according to claim 2, it is characterized in that including the following steps: the microbial composite bacteria of claim 1
Fertilizer is according to 35-45ml/m2Dosage be applied at depth of soil 5-10cm；By 24-26 hours, planting plants seedling.
4. application according to claim 3, it is characterized in that the plant is corn, Chinese rose or tobacco.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811048010.7A CN109294941A (en) | 2018-09-10 | 2018-09-10 | A kind of microbial compound bacterial fertilizer and the application in promotion plant absorption N P and K |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811048010.7A CN109294941A (en) | 2018-09-10 | 2018-09-10 | A kind of microbial compound bacterial fertilizer and the application in promotion plant absorption N P and K |
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| Publication Number | Publication Date |
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| CN109294941A true CN109294941A (en) | 2019-02-01 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201811048010.7A Pending CN109294941A (en) | 2018-09-10 | 2018-09-10 | A kind of microbial compound bacterial fertilizer and the application in promotion plant absorption N P and K |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101768020A (en) * | 2008-12-31 | 2010-07-07 | 丁海波 | Biological germ fertilizer as well as preparation method and application thereof |
| CN103992963A (en) * | 2014-03-19 | 2014-08-20 | 南京博农生物科技有限公司 | Bacillus megaterium and application thereof |
| CN105385638A (en) * | 2015-12-21 | 2016-03-09 | 云南省微生物发酵工程研究中心有限公司 | Microbial phosphorus-dissolving preparation and preparation method and application thereof |
| CN105439766A (en) * | 2015-12-09 | 2016-03-30 | 山东新富瑞农业科技有限公司 | Novel microbial fertilizer |
| CN107721713A (en) * | 2016-08-12 | 2018-02-23 | 南京轩凯生物科技有限公司 | A kind of microbial compound bacterial fertilizer and preparation method and application |
| WO2018129016A1 (en) * | 2017-01-04 | 2018-07-12 | Novozymes Bioag A/S | Bacillus isolates and uses thereof |
-
2018
- 2018-09-10 CN CN201811048010.7A patent/CN109294941A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101768020A (en) * | 2008-12-31 | 2010-07-07 | 丁海波 | Biological germ fertilizer as well as preparation method and application thereof |
| CN103992963A (en) * | 2014-03-19 | 2014-08-20 | 南京博农生物科技有限公司 | Bacillus megaterium and application thereof |
| CN105439766A (en) * | 2015-12-09 | 2016-03-30 | 山东新富瑞农业科技有限公司 | Novel microbial fertilizer |
| CN105385638A (en) * | 2015-12-21 | 2016-03-09 | 云南省微生物发酵工程研究中心有限公司 | Microbial phosphorus-dissolving preparation and preparation method and application thereof |
| CN107721713A (en) * | 2016-08-12 | 2018-02-23 | 南京轩凯生物科技有限公司 | A kind of microbial compound bacterial fertilizer and preparation method and application |
| WO2018129016A1 (en) * | 2017-01-04 | 2018-07-12 | Novozymes Bioag A/S | Bacillus isolates and uses thereof |
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Application publication date: 20190201 |
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