CN103918716A - Brevibacillus laterosporus-containing efficient pesticide composition - Google Patents
Brevibacillus laterosporus-containing efficient pesticide composition Download PDFInfo
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Abstract
The present invention discloses a brevibacillus laterosporus-containing efficient pesticide composition, wherein effective active components comprise an active component A and an active component B, the active component A is selected from brevibacillus laterosporus, and the active component B is any one compound selected from amino polysaccharide, chitosan, plant activator protein, lentinan, glucosan and amino-oligosaccharin, and a weight ratio of the active component A to the active component B is 1:300-500:1. The composition provides high activity for a plurality of plant diseases on a plurality of crops, and has characteristics of stimulation of crop root system development, promotion of crop growth, increase of yield, and improvement of crop quality.
Description
Technical field
The invention belongs to technical field of pesticide, relate to the application of a kind of highly effective pesticide composition containing Brevibacillus laterosporus in crop pest.
Technical background
Brevibacillus laterosporus is new type bactericide, can be used for preventing and treating multiple diseases, for example Phytophthora capsici disease, watermelon blight, wheat scab, grape grey mould, Strawberry anthracnose.
In the real process of agricultural production, preventing and treating the problem that disease the most easily produces is the generation of disease drug resistance.Different cultivars composition carries out composite, is to prevent and treat the very common method of resistance disease.Heterogeneity is carried out composite, according to practical application effect, judge certain composite be synergy, add and or antagonism.In most cases, the composite effect of agricultural chemicals is all additive effect, really has the seldom composite of synergistic effect, especially synergistic effect very obviously, synergy ratio very high composite just still less.Through inventor's research, discovery is mutually composite by Brevibacillus laterosporus and glycosaminoglycan, chitosan, toVerticilliumdahliaActivitie Activitie S of Relative activator protein, lentinan, Portugal's polyene sugar, amino-oligosaccharide, there is within the specific limits good synergistic effect, and about Brevibacillus laterosporus is to glycosaminoglycan, chitosan, toVerticilliumdahliaActivitie Activitie S of Relative activator protein, lentinan, Portugal's polyene is sugared, amino-oligosaccharide is relevant composite, there is not yet at present relevant report at home and abroad.
Summary of the invention
The object of the invention is to propose a kind of have synergistic function, use cost is low, preventive effect the is good highly effective pesticide composition containing Brevibacillus laterosporus.
The present invention is achieved through the following technical solutions:
A kind of highly effective pesticide composition containing Brevibacillus laterosporus, contain active components A and active component B, active components A and active component B weight ratio are 1: 300～500: 1, described active components A is selected from Brevibacillus laterosporus, and active component B is selected from a kind of in glycosaminoglycan, chitosan, toVerticilliumdahliaActivitie Activitie S of Relative activator protein, lentinan, Portugal's polyene sugar or amino-oligosaccharide; The weight ratio that is preferably active components A and active component B is 1 ︰ 50～300 ︰ 1; More preferably the weight ratio of Brevibacillus laterosporus and glycosaminoglycan or chitosan or toVerticilliumdahliaActivitie Activitie S of Relative activator protein or lentinan is 1: 30～60: 1, the weight ratio of Brevibacillus laterosporus and Portugal's polyene sugar is 1: 20～80: 1, and the weight ratio of Brevibacillus laterosporus and amino-oligosaccharide is 1: 20～50: 1.
Bactericidal composition of the present invention, can be processed on demand acceptable formulation in any sterilization, wherein more preferably formulation is wetting powder, aqua, water dispersible granules, soluble powder, soluble liquid, soluble granule, can also make suspending agent, suspension emulsion, aqueous emulsion, microemulsion, microcapsule suspending agent, microcapsule suspension-suspending agent, seed coat agent, effervescent agent.
The disease of the described highly effective pesticide composition containing Brevibacillus laterosporus for preventing and treating crop; Described crop is cereal crops, legume crop, fiber crop, sugar [yielding, melon crop, fruits crop, dry fruit crop, hobby crop, root crop, oil crop, flowers crop, medicinal crop, raw material crop, green manure pasture crop; Described disease is virus disease, powdery mildew, downy mildew, epidemic disease, head blight, fusarium wilt, anthracnose, gray mold, soft rot or stripe virus disease.
The amount of application when content of active component depends on independent use in bactericidal composition of the present invention, also depends on mixed ratio and the synergistic effect degree of a kind of compound and another kind of compound, and the while is also relevant with target disease.Conventionally in composition, the weight percentage of active component is gross weight 1%～90%, is preferably 5%～80%.According to different preparation types, active component content scope is different.Liquids in general preparation contains 1%～60% active substance by weight, is preferably 5%～50%; Solid pharmaceutical preparation contains 5%～80% active substance by weight, is preferably 10%～80%.
In bactericidal composition of the present invention, at least contain a kind of surfactant, the dispersion of active component in water while being beneficial to use.Surface-active contents is 2%～30% of total formulation weight amount, and surplus is solid or liquid diluent.
Bactericidal composition of the present invention can be by user before use through dilution or directly use.Its preparation can be prepared by processing method known in those skilled in the art, after being about to active component and mixing with liquid flux or solid carrier, then add surfactant as one or more in dispersant, stabilizing agent, wetting agent, binding agent, disintegrant, defoamer etc.According to different dosage form, in preparation, can also contain antifreeze known in those skilled in the art etc.
When making wetting powder, composition comprises following component and content: active components A 0.001% ~ 80%, active component B0.001% ~ 60%, dispersant 1% ~ 12%, wetting agent 1% ~ 8%, filler surplus.
When making aqua, composition comprises following constituent content: active components A 0.001% ~ 50%, active component B0.001% ~ 50%, cosolvent 2% ~ 6%, wetting agent 1%-10%, antifreeze 0 ~ 8%, deionized water add to 100%.
When making soluble powder, composition comprises following constituent content: active components A 0.001% ~ 80%, active component B0.001% ~ 60%, dispersant 2% ~ 8%, wetting agent 1%-7%, stabilizing agent 0 ~ 5%, filler surplus.
When making soluble liquid, composition comprises following constituent content: active components A 0.001% ~ 50%, active component B0.001% ~ 50%, emulsifier 1% ~ 10%, cosolvent 2% ~ 6%, solvent add to 100%.
When making water dispersible granules, composition comprises following component and content: active components A 0.001% ~ 80%, active component B0.001% ~ 60%, dispersant 1% ~ 12%, wetting agent 1% ~ 8%, disintegrant 1% ~ 10%, binding agent 0 ~ 8%, filler surplus.
When making soluble granule, composition comprises following constituent content: active component
A0.001% ~ 80%, active component B0.001% ~ 60%, binding agent 0 ~ 8%, colouring agent
0.1%-2%, dispersant 2% ~ 10%, wetting agent 2% ~ 10%, filler surplus.
Wetting powder the key technical indexes of the present invention:
Aqua the key technical indexes of the present invention:
Soluble powder the key technical indexes of the present invention:
Soluble liquid the key technical indexes of the present invention:
Water dispersible granules the key technical indexes of the present invention:
Soluble granule the key technical indexes of the present invention:
The invention has the advantages that:
(1) present composition has good synergy and lasting effect within the specific limits, and preventive effect is higher than single dose; (2) agricultural chemicals dosage reduces, and reduces the residual quantity of agricultural chemicals on crop, alleviates environmental pollution; (3) expanded fungicidal spectrum, multiple diseases is had to greater activity as virus disease, powdery mildew, downy mildew, epidemic disease, head blight, fusarium wilt, anthracnose, gray mold, soft rot or stripe virus disease etc., and have and stimulate crop root to grow, promote plant growth, increase output, improve the feature of crop quality.
Embodiment
Below in conjunction with embodiment, to further instruction of the present invention, the percentage in embodiment is all weight percentage, but the present invention is not limited thereto.
Application Example one
Embodiment 1～18 wetting powder
Brevibacillus laterosporus, active component B, dispersant, wetting agent, filler are mixed, in mixed cylinder, mix, after airslide disintegrating mill is pulverized, mix again, can be made into wetting powder product of the present invention.Specifically in Table 1.
Table 1 embodiment 1～18 each component and content
Example 19～36 aquas
Brevibacillus laterosporus, active component B, wetting agent, antifreeze, solvent, deionized water etc. are mixed together, make the aqua product of the present composition, specifically in Table 2.
Table 2 embodiment 19～36 each component and content
Embodiment 3754 soluble powders
Brevibacillus laterosporus, active component B, dispersant, wetting agent, stabilizing agent (can add and can not add), filler are mixed in mixed cylinder, after pulverizing, airslide disintegrating mill mixes again, can make soluble powder product of the present invention, specifically in Table 3.
Table 3 embodiment 3754 each component and content
Embodiment 55～72 soluble liquids
Emulsifier, cosolvent, defoamer are mixed through high speed shear, add Brevibacillus laterosporus, active component B, in ball mill, ball milling is 2 ~ 3 hours, make diameter of particle all below 5 μ m, surplus is supplied with solvent, can make soluble liquid product of the present invention, specifically in Table 4.
Table 4 embodiment 55～72 each component and content
Embodiment 73～84 water dispersible granules
Brevibacillus laterosporus, active component B, dispersant, wetting agent, disintegrant, filler etc. are obtained to the particle diameter needing through air-flow crushing together, then add other auxiliary agents such as binding agent (can add and can not add), obtain the materials of granulating.By item quantitatively send in fluidized bed granulation dryer through granulation and dry after, can make water dispersible granules product of the present invention, specifically in Table 5.
Table 5 example 73～84 components and content
Embodiment 87～98 soluble granules
The withered prestige of spiral shell, active component B, binding agent (can add and can not add), colouring agent, dispersant, wetting agent, filler are mixed in mixed cylinder, at process extruder grain, dry, and make granule product of the present invention through screening, specifically in Table 6.
Table 6 embodiment 87～98 each component and weight portions
Glycosaminoglycan, chitosan, toVerticilliumdahliaActivitie Activitie S of Relative activator protein, lentinan, Portugal's polyene sugar, amino-oligosaccharide in table 1 ~ 5 are exchanged, can make novel formulation.
The embodiment of the present invention is the method that adopts Toxicity Determination and field trial to combine.First by Toxicity Determination, the synergy ratio (SR) after clear and definite two kinds of medicaments are composite by a certain percentage, SR < 0.5 is antagonism, 0.5≤SR≤1.5 are summation action, SR > 1.5 is synergistic effect, on this basis, then carries out field trial.
Test method: after the effective inhibition concentration scope of each medicament is determined in prerun, medicament is established respectively 5 dosage by active constituent content and processed, and establishes clear water contrast.With reference to < < farm-chemical indoor determination test rule bactericide > >, carry out, adopt mycelial growth rate method to measure the virulence of medicament to crop germ.After 72h, by right-angled intersection method, measure colony diameter, calculate respectively process net growth,
Mycelial growth inhibition rate.
Net growth (mm)=measurement colony diameter-5
Mycelial growth inhibition rate is converted into probit value (y), and liquor strength (μ g/mL) converts logarithm value to (x), with method of least squares, tries to achieve virulence regression equation (y=a+bx), and calculates thus the EC of every kind of medicament
50value.According to Wadley method, calculate the different proportioning synergy ratio (SR) of two medicaments, SR < 0.5 is antagonism simultaneously, and 0.5≤SR≤1.5 are summation action, and SR > 1.5 is synergistic effect.Computing formula is as follows:
Wherein: a, b are respectively active components A and active component B shared ratio in combination;
A is Brevibacillus laterosporus;
B is selected from a kind of in glycosaminoglycan, chitosan, toVerticilliumdahliaActivitie Activitie S of Relative activator protein, lentinan, Portugal's polyene sugar or amino-oligosaccharide.
Application Example two:
For trying disease: capsicum epidemic disease, test medicine provides by Shanxi Thompson Biological Technology Co., Ltd., experimental scheme: effective inhibition concentration scope of determining the two different proportioning mixture of Brevibacillus laterosporus and glycosaminoglycan through preliminary experiment.
Toxicity test result
The composite toxicity test analysis of results table to capsicum epidemic disease of table 7 Brevibacillus laterosporus and glycosaminoglycan
As shown in Table 7, the proportioning of the composite control capsicum epidemic disease of Brevibacillus laterosporus and glycosaminoglycan is when 1 ︰ 300 ~ 500 ︰ 1, synergy ratio SR is all greater than 1.5, illustrate that both are mixed and all show synergistic effect in 1 ︰ 300 ~ 500 ︰ 1 scopes, the proportioning of Brevibacillus laterosporus and glycosaminoglycan was at 1: 30～60: 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.20.Through applicant, test and find that the proportioning of Brevibacillus laterosporus and glycosaminoglycan is 1 ︰ 30, 1 ︰ 25, 1 ︰ 20, 1 ︰ 15, 1 ︰ 10, 1 ︰ 5, 1 ︰ 1, 4 ︰ 1, 5 ︰ 1, 6 ︰ 1, 7 ︰ 1, 8 ︰ 1, 9 ︰ 1, 10 ︰ 1, 11 ︰ 1, 12 ︰ 1, 13 ︰ 1, 14 ︰ 1, 15 ︰ 1, 16 ︰ 1, 17 ︰ 1, 18 ︰ 1, 19 ︰ 1, 20 ︰ 1, 21 ︰ 1, 22 ︰ 1, 23 ︰ 1, 24 ︰ 1, 25 ︰ 1, 29 ︰ 1, 30 ︰ 1, 35 ︰ 1, 40 ︰ 1, 45 ︰ 1, 50 ︰ 1, 55 ︰ 1, 60 ︰ 1, when wherein Brevibacillus laterosporus and glycosaminoglycan weight ratio are 10:1, synergy ratio is maximum, synergistic effect is the most obvious.
Application Example three:
For trying disease: powdery mildew of cucumber, test medicine provides by Shanxi Thompson Biological Technology Co., Ltd., experimental scheme: effective inhibition concentration scope of determining the former medicine of Brevibacillus laterosporus and chitosan and the two different proportioning mixture through preliminary experiment.
Toxicity test result
The composite toxicity test analysis of results table to powdery mildew of cucumber of table 8 Brevibacillus laterosporus and chitosan
As shown in Table 8, the composite proportioning of preventing and treating powdery mildew of cucumber of Brevibacillus laterosporus and chitosan is when 1 ︰ 300 ~ 500 ︰ 1, synergy ratio SR is all greater than 1.5, illustrate that both are mixed and all show synergistic effect in 1 ︰ 300 ~ 500 ︰ 1 scopes, the proportioning of Brevibacillus laterosporus and chitosan was at 1: 30～60: 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.20.Through applicant, test and find that the proportioning of Brevibacillus laterosporus and chitosan is 1 ︰ 30, 1 ︰ 25, 1 ︰ 20, 1 ︰ 15, 1 ︰ 10, 1 ︰ 5, 1 ︰ 1, 4 ︰ 1, 5 ︰ 1, 6 ︰ 1, 7 ︰ 1, 8 ︰ 1, 9 ︰ 1, 10 ︰ 1, 11 ︰ 1, 12 ︰ 1, 13 ︰ 1, 14 ︰ 1, 15 ︰ 1, 16 ︰ 1, 17 ︰ 1, 18 ︰ 1, 19 ︰ 1, 20 ︰ 1, 21 ︰ 1, 22 ︰ 1, 23 ︰ 1, 24 ︰ 1, 25 ︰ 1, 29 ︰ 1, 30 ︰ 1, 35 ︰ 1, 40 ︰ 1, 45 ︰ 1, 50 ︰ 1, 55 ︰ 1, 60 ︰ 1, when wherein Brevibacillus laterosporus and chitosan weight ratio are 11:1, synergy ratio is maximum, synergistic effect is the most obvious.
Application Example four:
For trying disease: tomato virus disease, test medicine provides by Shanxi Thompson Biological Technology Co., Ltd., experimental scheme: effective inhibition concentration scope of determining Brevibacillus laterosporus and the former medicine of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and the two different proportioning mixture through preliminary experiment.
Toxicity test result
The composite toxicity test analysis of results table to tomato virus disease of table 9 Brevibacillus laterosporus and toVerticilliumdahliaActivitie Activitie S of Relative activator protein
As shown in Table 9, the proportioning of Brevibacillus laterosporus and the composite control tomato virus disease of toVerticilliumdahliaActivitie Activitie S of Relative activator protein is when 1 ︰ 300 ~ 500 ︰ 1, synergy ratio SR is all greater than 1.5, illustrate that both are mixed and all show synergistic effect in 1 ︰ 300 ~ 500 ︰ 1 scopes, the proportioning of Brevibacillus laterosporus and toVerticilliumdahliaActivitie Activitie S of Relative activator protein was at 1: 30～60: 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.20.Through applicant, test and find that the proportioning of Brevibacillus laterosporus and toVerticilliumdahliaActivitie Activitie S of Relative activator protein is 1 ︰ 30, 1 ︰ 25, 1 ︰ 20, 1 ︰ 15, 1 ︰ 10, 1 ︰ 5, 1 ︰ 1, 4 ︰ 1, 5 ︰ 1, 6 ︰ 1, 7 ︰ 1, 8 ︰ 1, 9 ︰ 1, 10 ︰ 1, 11 ︰ 1, 12 ︰ 1, 13 ︰ 1, 14 ︰ 1, 15 ︰ 1, 16 ︰ 1, 17 ︰ 1, 18 ︰ 1, 19 ︰ 1, 20 ︰ 1, 21 ︰ 1, 22 ︰ 1, 23 ︰ 1, 24 ︰ 1, 25 ︰ 1, 29 ︰ 1, 30 ︰ 1, 35 ︰ 1, 40 ︰ 1, 45 ︰ 1, 50 ︰ 1, 55 ︰ 1, 60 ︰ 1, when wherein Brevibacillus laterosporus and toVerticilliumdahliaActivitie Activitie S of Relative activator protein weight ratio are 15:1, synergy ratio is maximum, synergistic effect is the most obvious.
Application Example five:
For trying disease: pepper virus disease, test medicine provides by Shanxi Thompson Biological Technology Co., Ltd., experimental scheme: effective inhibition concentration scope of determining the former medicine of Brevibacillus laterosporus and lentinan and the two different proportioning mixture through preliminary experiment.
Toxicity test result
The composite toxicity test analysis of results table to pepper virus disease of table 10 Brevibacillus laterosporus and lentinan
As shown in Table 10, the proportioning of the composite control pepper virus disease of Brevibacillus laterosporus and lentinan is when 1 ︰ 300 ~ 500 ︰ 1, synergy ratio SR is all greater than 1.5, illustrate that both are mixed and all show synergistic effect in 1 ︰ 300 ~ 500 ︰ 1 scopes, the proportioning of Brevibacillus laterosporus and lentinan was at 1: 30～60: 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.25.Through applicant, test and find that the proportioning of Brevibacillus laterosporus and lentinan is 1 ︰ 30, 1 ︰ 25, 1 ︰ 20, 1 ︰ 15, 1 ︰ 10, 1 ︰ 5, 1 ︰ 1, 4 ︰ 1, 5 ︰ 1, 6 ︰ 1, 7 ︰ 1, 8 ︰ 1, 9 ︰ 1, 10 ︰ 1, 11 ︰ 1, 12 ︰ 1, 13 ︰ 1, 14 ︰ 1, 15 ︰ 1, 16 ︰ 1, 17 ︰ 1, 18 ︰ 1, 19 ︰ 1, 20 ︰ 1, 21 ︰ 1, 22 ︰ 1, 23 ︰ 1, 24 ︰ 1, 25 ︰ 1, 29 ︰ 1, 30 ︰ 1, 35 ︰ 1, 40 ︰ 1, 45 ︰ 1, 50 ︰ 1, 55 ︰ 1, 60 ︰ 1, when wherein Brevibacillus laterosporus and lentinan weight ratio are 16:1, synergy ratio is maximum, synergistic effect is the most obvious.
Application Example six:
For examination disease: tomato blight, test medicine provides by Shanxi Thompson Biological Technology Co., Ltd., experimental scheme: effective inhibition concentration scope of determining Brevibacillus laterosporus and Portugal's polyene glycogen medicine and the two different proportioning mixture through preliminary experiment.
Toxicity test result
Table 11 Brevibacillus laterosporus and the composite toxicity test analysis of results table to tomato blight of Portugal's polyene sugar
As shown in Table 11, the proportioning of Brevibacillus laterosporus and the composite control tomato blight of Portugal's polyene sugar is when 1 ︰ 300 ~ 500 ︰ 1, synergy ratio SR is all greater than 1.5, illustrate that both are mixed and all show synergistic effect in 1 ︰ 300 ~ 500 ︰ 1 scopes, the proportioning of Brevibacillus laterosporus and Portugal's polyene sugar was at 1: 20～80: 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.25.Through applicant, test and find that the proportioning of Brevibacillus laterosporus and Portugal's polyene sugar is 1 ︰ 20, 1 ︰ 15, 1 ︰ 10, 1 ︰ 5, 1 ︰ 1, 4 ︰ 1, 5 ︰ 1, 6 ︰ 1, 7 ︰ 1, 8 ︰ 1, 9 ︰ 1, 10 ︰ 1, 11 ︰ 1, 12 ︰ 1, 13 ︰ 1, 14 ︰ 1, 15 ︰ 1, 16 ︰ 1, 17 ︰ 1, 18 ︰ 1, 19 ︰ 1, 20 ︰ 1, 21 ︰ 1, 22 ︰ 1, 23 ︰ 1, 24 ︰ 1, 25 ︰ 1, 29 ︰ 1, 30 ︰ 1, 35 ︰ 1, 40 ︰ 1, 45 ︰ 1, 50 ︰ 1, 55 ︰ 1, 60 ︰ 1, 70 ︰ 1, 80 ︰ 1, when wherein Brevibacillus laterosporus is 19:1 with Portugal's polyene sugar weight ratio, synergy ratio is maximum, synergistic effect is the most obvious.
Application Example seven:
For trying disease: watermelon blight, test medicine provides by Shanxi Thompson Biological Technology Co., Ltd., experimental scheme: effective inhibition concentration scope of determining the former medicine of Brevibacillus laterosporus and amino-oligosaccharide and the two different proportioning mixture through preliminary experiment.
Toxicity test result
The composite toxicity test analysis of results table to watermelon blight of table 12 Brevibacillus laterosporus and amino-oligosaccharide
As shown in Table 12, the proportioning of the composite control watermelon blight of Brevibacillus laterosporus and amino-oligosaccharide is when 1 ︰ 300 ~ 500 ︰ 1, synergy ratio SR is all greater than 1.5, illustrate that both are mixed and all show synergistic effect in 1 ︰ 300 ~ 500 ︰ 1 scopes, the proportioning of Brevibacillus laterosporus and amino-oligosaccharide was at 1: 20～50: 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.30.Through applicant, test and find that the proportioning of Brevibacillus laterosporus and amino-oligosaccharide is 1 ︰ 20,1 ︰ 15,1 ︰ 10,1 ︰ 5,1 ︰ 1,4 ︰ 1,5 ︰ 1,6 ︰ 1,7 ︰ 1,8 ︰ 1,9 ︰ 1,10 ︰ 1,11 ︰ 1,12 ︰ 1,13 ︰ 1,14 ︰ 1,15 ︰ 1,16 ︰ 1,17 ︰ 1,18 ︰ 1,19 ︰ 1,20 ︰ 1,21 ︰ 1,22 ︰ 1,23 ︰ 1,24 ︰ 1,25 ︰ 1,29 ︰ 1,30 ︰ 1,35 ︰ 1,40 ︰ 1,45 ︰ 1,50 ︰ 1, when wherein Brevibacillus laterosporus and amino-oligosaccharide weight ratio are 4:1, synergy ratio is maximum, and synergistic effect is the most obvious.
Application Example eight:
For studying thing: tomato, test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..Experimental scheme: the concentration range of determining the two different proportioning mixture coordinate plant growth of Brevibacillus laterosporus and active component B through preliminary experiment.
Toxicity test result
The composite growth regulation effect to tomato of table 13 Brevibacillus laterosporus and active component B
As shown in Table 13, Brevibacillus laterosporus mixes with active component B after use, metabolic system that can activated plant and plant growth quickening vigor is strengthened, the increase of proline content and the reinforcement of cellulase, promote plant cell to increase, and stimulates crop root to grow, promote plant growth, increase output, improve crop quality, Brevibacillus laterosporus and chitosan, when 1 ︰ 300 ~ 500 ︰ 1, all have stronger regulating action.
Through test, find: after Brevibacillus laterosporus and active component B are composite, the control of the virus disease in various crop, powdery mildew, downy mildew, epidemic disease, head blight, fusarium wilt, anthracnose, gray mold, soft rot or stripe virus disease is had to obvious synergistic effect, synergy ratio is all more than 1.5, and there is the crop root of stimulation to grow, promote plant growth, increase output, improve the feature of crop quality.
Effect experiment part: test medicine is researched and developed, provided by Shanxi Thompson Biological Technology Co., Ltd., contrast medicament 10,000,000,000 spores/gram Brevibacillus laterosporus wetting powder (autogamy), 2% glycosaminoglycan aqua (autogamy), 2% chitosan aqua (commercial), 3% toVerticilliumdahliaActivitie Activitie S of Relative activate egg wetting powder (commercial), 1% lentinan aqua (commercial), 0.5% Portugal's polyene sugar soluble powder (commercial), 2% amino-oligosaccharide aqua (commercial).
Application Example nine Brevibacillus laterosporus and active component B and composite control tomato blight drug effect and growth regulation Experiment on Function
This experimental establishment, in Xi'an City, Shanxi Province, is investigated the tomato blight state of an illness before medicine, in the dispenser for the first time of state of an illness initial stage, once, dispenser is 2 times altogether in dispenser in every 7 days.Within after dispenser 7 days, 14 days, 30 days for the second time, investigate respectively disease index and calculate preventive effect.Experimental result is as follows:
Table 14 Brevibacillus laterosporus and active component B and composite control tomato blight drug effect and growth regulation Experiment on Function
As can be seen from Table 14, Brevibacillus laterosporus and active component B and composite after can effectively prevent and treat tomato blight, control efficiency is all better than the preventive effect of single dose, and the preventive effect phase is long.In test scope of medication, target crop is had no adverse effects.
Application Example ten Brevibacillus laterosporus and active component B and composite powdery mildew of cucumber drug effect and the growth regulation Experiment on Function prevented and treated thereof
This test arrangement, in Jingyang County, Shaanxi Province, is investigated the powdery mildew of cucumber state of an illness before medicine, in the dispenser for the first time of state of an illness initial stage, once, dispenser is 2 times altogether in dispenser in every 7 days.Within after dispenser 3 days, 7 days, 15 days for the second time, investigate respectively disease index and calculate preventive effect.Experimental result is as follows:
Table 15 Brevibacillus laterosporus and active component B and composite powdery mildew of cucumber drug effect and the growth regulation Experiment on Function prevented and treated thereof
As can be seen from Table 15, Brevibacillus laterosporus and active component B and composite after can effectively prevent and treat powdery mildew of cucumber, control efficiency is all better than the preventive effect of single dose, and the preventive effect phase is long, in test, target crop is had no adverse effects in scope of medication.
Application Example 11 Brevibacillus laterosporus and active component B and composite control watermelon blight drug effect and growth regulation Experiment on Function
This test arrangement, in Dali County, Shaanxi Province, is investigated the watermelon blight state of an illness before medicine, in the dispenser for the first time of state of an illness initial stage, once, dispenser is 2 times altogether in dispenser in every 7 days.Within after dispenser 7 days, 14 days, 30 days for the second time, investigate respectively disease index and calculate preventive effect.Experimental result is as follows:
Table 16 Brevibacillus laterosporus and active component B and composite control watermelon blight drug effect and growth regulation Experiment on Function
As can be seen from Table 16, Brevibacillus laterosporus and active component B and composite after can effectively prevent and treat watermelon blight, control efficiency is all better than the preventive effect of single dose, and the preventive effect phase is long.In test scope of medication, target crop is had no adverse effects.
Application Example 12 Brevibacillus laterosporus and active component B and composite control tomato virus disease drug effect and growth regulation Experiment on Function
This test arrangement, in Xi'an City, Shanxi Province, is investigated the tomato virus disease state of an illness before medicine, in the dispenser for the first time of state of an illness initial stage, once, dispenser is 2 times altogether in dispenser in every 7 days.Within after dispenser 7 days, 14 days, 30 days for the second time, investigate respectively disease index and calculate preventive effect.Experimental result is as follows:
Table 17 Brevibacillus laterosporus and active component B and composite control tomato virus disease drug effect and growth regulation Experiment on Function
As can be seen from Table 17, Brevibacillus laterosporus and active component B and composite after can effectively prevent and treat tomato virus disease, control efficiency is all better than the preventive effect of single dose, and the preventive effect phase is long.In test scope of medication, target crop is had no adverse effects.
Application Example 13 Brevibacillus laterosporus and active component B and composite wheat scab drug effect and the growth regulation Experiment on Function prevented and treated thereof
This test arrangement, at Shaanxi Province's Weinan City, is investigated the wheat scab state of an illness before medicine, in the dispenser for the first time of state of an illness initial stage, once, dispenser is 2 times altogether in dispenser in every 7 days.Within after dispenser 7 days, 14 days, 30 days for the second time, investigate respectively disease index and calculate preventive effect.Experimental result is as follows:
Table 18 Brevibacillus laterosporus and active component B and composite wheat scab drug effect and the growth regulation Experiment on Function prevented and treated thereof
As can be seen from Table 18, Brevibacillus laterosporus and active component B and composite after can effectively prevent and treat wheat scab, control efficiency is all better than the preventive effect of single dose, and the preventive effect phase is long.In test scope of medication, target crop is had no adverse effects.
Application Example 14 Brevibacillus laterosporus and active component B and composite control Strawberry anthracnose drug effect and growth regulation Experiment on Function
This test arrangement, at Shaanxi Province's Weinan City, is investigated the Strawberry anthracnose state of an illness before medicine, in the dispenser for the first time of state of an illness initial stage, once, dispenser is 2 times altogether in dispenser in every 7 days.Within after dispenser 7 days, 14 days, 30 days for the second time, investigate respectively disease index and calculate preventive effect.Experimental result is as follows:
Table 19 Brevibacillus laterosporus and active component B and composite control Strawberry anthracnose drug effect and growth regulation Experiment on Function
As can be seen from Table 19, Brevibacillus laterosporus and active component B and composite after can effectively prevent and treat Strawberry anthracnose, control efficiency is all better than the preventive effect of single dose, and the preventive effect phase is long.In test scope of medication, target crop is had no adverse effects.
Application Example 15 Brevibacillus laterosporus and active component B and composite control capsicum epidemic disease drug effect and growth regulation Experiment on Function
This test arrangement, in Xianyang, Shanxi province city, is investigated the capsicum epidemic disease state of an illness before medicine, in the dispenser for the first time of state of an illness initial stage, once, dispenser is 2 times altogether in dispenser in every 7 days.Within after dispenser 7 days, 14 days, 30 days for the second time, investigate respectively disease index and calculate preventive effect.Experimental result is as follows:
Table 20 Brevibacillus laterosporus and active component B and composite control capsicum epidemic disease drug effect and growth regulation Experiment on Function
As can be seen from Table 20, Brevibacillus laterosporus and active component B and composite after can effectively prevent and treat virus disease, powdery mildew, downy mildew, epidemic disease, head blight, fusarium wilt, anthracnose, gray mold, soft rot or stripe virus disease, control efficiency is all better than the preventive effect of single dose, and the preventive effect phase is long.In test scope of medication, target crop is had no adverse effects.
After through different local throughout the country tests, draw, glycosaminoglycan, chitosan, toVerticilliumdahliaActivitie Activitie S of Relative activator protein, lentinan, Portugal's polyene sugar or amino-oligosaccharide composite rear to the preventive effect of the Common Diseases such as the virus disease in various crop, powdery mildew, downy mildew, epidemic disease, head blight, fusarium wilt, anthracnose, gray mold, soft rot or stripe virus disease all more than 95%, be better than single dose preventive effect, synergistic effect is obvious, and have and stimulate crop root to grow, promote plant growth, increase output, improve the feature of crop quality.
Claims (7)
1. the highly effective pesticide composition containing Brevibacillus laterosporus, effective active composition is active components A and active component B, it is characterized in that: the weight ratio of active components A and active component B is 1: 300～500: 1, described active components A is selected from Brevibacillus laterosporus, and active component B is selected from a kind of in glycosaminoglycan, chitosan, toVerticilliumdahliaActivitie Activitie S of Relative activator protein, lentinan, Portugal's polyene sugar or amino-oligosaccharide.
2. the highly effective pesticide composition containing Brevibacillus laterosporus according to claim 1, is characterized in that: the weight ratio of active components A and active component B is 1: 50～300: 1.
3. the highly effective pesticide composition containing Brevibacillus laterosporus according to claim 2, is characterized in that:
The weight ratio of Brevibacillus laterosporus and glycosaminoglycan or chitosan or toVerticilliumdahliaActivitie Activitie S of Relative activator protein or lentinan is 1: 30～60: 1;
The weight ratio of Brevibacillus laterosporus and Portugal's polyene sugar is 1: 20～80: 1;
The weight ratio of Brevibacillus laterosporus and amino-oligosaccharide is 1: 20～50: 1.
4. the highly effective pesticide composition containing Brevibacillus laterosporus according to claim 1, is characterized in that: the content of Brevibacillus laterosporus be 100,000,000～50,000,000,000 living spores/gram, the content of active component B is 0.01%～20%.
5. according to the highly effective pesticide composition containing Brevibacillus laterosporus described in any one in claim 1～4, it is characterized in that: composition is made wetting powder, aqua, water dispersible granules, soluble powder, soluble liquid or soluble granule.
6. the highly effective pesticide composition containing Brevibacillus laterosporus according to claim 1 is used for the purposes of preventing and treating crop pest and Promoting plant growth.
7. purposes according to claim 6, is characterized in that: described disease is virus disease, powdery mildew, downy mildew, epidemic disease, head blight, fusarium wilt, anthracnose, gray mold, soft rot or stripe virus disease.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104255805A (en) * | 2014-08-13 | 2015-01-07 | 江苏丘陵地区镇江农业科学研究所 | Biological sterilization composition of chitosan and bacillus amyloliquefaciens and application thereof |
| CN104542716A (en) * | 2014-12-30 | 2015-04-29 | 天津市工业微生物研究所有限公司 | Novel biological seed coating agent and preparation method thereof |
| CN105454234A (en) * | 2015-12-08 | 2016-04-06 | 唐山金土生物有机肥有限公司 | Single or compound oligochitosan preparation and application of single or compound oligochitosan preparation in tomato virus disease control |
| CN106070245B (en) * | 2016-08-15 | 2018-08-28 | 江苏辉丰农化股份有限公司 | A kind of microbicide compositions |
| CN110122482A (en) * | 2019-05-08 | 2019-08-16 | 北京普绿通生物肥料厂 | A kind of preparation process of toVerticilliumdahliaActivitie Activitie S of Relative PeaT1 plant immune protein soluble powder |
| CN113229293A (en) * | 2021-05-07 | 2021-08-10 | 河北中保绿农作物科技有限公司 | Bactericidal composition and application thereof |
| CN114027323A (en) * | 2021-11-19 | 2022-02-11 | 天津市汉邦植物保护剂有限责任公司 | Brevibacillus laterosporus and Paenibacillus polymyxa combined granule as well as preparation method and application thereof |
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2013
- 2013-01-10 CN CN201310009194.7A patent/CN103918716A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104255805A (en) * | 2014-08-13 | 2015-01-07 | 江苏丘陵地区镇江农业科学研究所 | Biological sterilization composition of chitosan and bacillus amyloliquefaciens and application thereof |
| CN104542716A (en) * | 2014-12-30 | 2015-04-29 | 天津市工业微生物研究所有限公司 | Novel biological seed coating agent and preparation method thereof |
| CN105454234A (en) * | 2015-12-08 | 2016-04-06 | 唐山金土生物有机肥有限公司 | Single or compound oligochitosan preparation and application of single or compound oligochitosan preparation in tomato virus disease control |
| CN106070245B (en) * | 2016-08-15 | 2018-08-28 | 江苏辉丰农化股份有限公司 | A kind of microbicide compositions |
| CN110122482A (en) * | 2019-05-08 | 2019-08-16 | 北京普绿通生物肥料厂 | A kind of preparation process of toVerticilliumdahliaActivitie Activitie S of Relative PeaT1 plant immune protein soluble powder |
| CN110122482B (en) * | 2019-05-08 | 2021-09-10 | 北京普绿通生物肥料厂 | Preparation process of plant immune protein soluble powder of alternaria tenuissima PeaT1 |
| CN113229293A (en) * | 2021-05-07 | 2021-08-10 | 河北中保绿农作物科技有限公司 | Bactericidal composition and application thereof |
| CN114027323A (en) * | 2021-11-19 | 2022-02-11 | 天津市汉邦植物保护剂有限责任公司 | Brevibacillus laterosporus and Paenibacillus polymyxa combined granule as well as preparation method and application thereof |
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Application publication date: 20140716 |