CN103918715A - Brevibacillus laterosporus-containing antibacterial yield increase composition - Google Patents
Brevibacillus laterosporus-containing antibacterial yield increase composition Download PDFInfo
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Abstract
The present invention relates to a brevibacillus laterosporus-containing antibacterial yield increase composition, which is an antibacterial yield increase composition comprising effective active components such as an active component A and an active component B, wherein the active component A is selected from brevibacillus laterosporus, and the active component B is any one compound selected from benzylaminopurine, brassinolide, indole acetic acid, triacontanol and gibberellic acid. The composition provides high activity for a plurality of plant diseases on a plurality of crops, and has characteristics of significant synergy effect, bacterial killing spectrum expanding, increase of effective fertilizer absorption of crops, helping of good growth of crop inferior parts, and regulation of water balance in crops so as to activate metabolic systems of crops, and promote crop cell growth and root system activity strengthening.
Description
Technical field
The invention belongs to technical field of pesticide, relate to a kind of antibacterial volume increase composition containing Brevibacillus laterosporus in the application of crops.
Technical background
Brevibacillus laterosporus is new type bactericide, can be used for preventing and treating multiple diseases, for example capsicum epidemic disease, watermelon blight, wheat scab, grape grey mould, Strawberry anthracnose.
Benayl aminopurine, brassin lactones, heteroauxin, triacontanol, gibberellic acid are conventional plant growth regulator, and wherein gibberellic acid is gibberellic acid A4+A7, gibberellic acid A3, gibberellic acid A4, gibberellic acid A7.
In the real process of agricultural production, preventing and treating the problem that disease is easy to produce is, when having prevented and treated disease, the growth of plant itself is had to inhibitory action.By bactericide with plant growth regulator is composite can also promote the growth of plant roots and leaf when can effectively prevent and treat disease.Heterogeneity is carried out composite, according to practical application effect, judge certain composite be synergy, add and or antagonism.In most cases, the composite effect of agricultural chemicals is all additive effect, really has the seldom composite of synergistic effect, especially synergistic effect very obviously, synergy ratio very high composite just still less.Through inventor's research, discovery is mutually composite by Brevibacillus laterosporus and benayl aminopurine, brassin lactones, heteroauxin, triacontanol, gibberellic acid, there is within the specific limits good synergistic effect, can well endure the anti pathologic immunity power of high crop, strengthen the activity of root system, thereby reach the object of volume increase, and relevant composite about Brevibacillus laterosporus and benayl aminopurine, brassin lactones, heteroauxin, triacontanol, gibberellic acid, there is not yet at present relevant report at home and abroad.
Summary of the invention
The object of the invention is to propose a kind of have synergistic function, low, the effective antibacterial volume increase composition that contains Brevibacillus laterosporus of use cost.
The present invention is achieved through the following technical solutions:
A kind of antibacterial volume increase composition containing Brevibacillus laterosporus, composition is active components A and active component B, the weight ratio of active components A and active component B is 10000:1 ~ 1:100, described active components A is selected from Brevibacillus laterosporus, active component B is selected from a kind of in benayl aminopurine, brassin lactones, heteroauxin, triacontanol, gibberellic acid, and in composition, the more excellent weight ratio of active components A and active component B is 8000:1 ~ 1:50; Wherein the weight ratio of Brevibacillus laterosporus and benayl aminopurine is 50:1 ~ 1:20, the weight ratio of Brevibacillus laterosporus and brassin lactones is 3000:1 ~ 1000:1, the weight ratio of Brevibacillus laterosporus and heteroauxin is 6000:1 ~ 3000:1, the weight ratio of Brevibacillus laterosporus and triacontanol is 600:1 ~ 200:1, and the weight ratio of Brevibacillus laterosporus and gibberellic acid is 40:1 ~ 1:20.
Described plant growth regulator composition, wherein effective active component content accounts for 0.0001%～60% of gross weight, is preferably 0.001%～20%.
The purposes of the described antibacterial volume increase composition containing Brevibacillus laterosporus for preventing and treating crop pest and Promoting plant growth.
Described crop is pears, apple, grape, oranges and tangerines, banana, strawberry, watermelon, Chinese cabbage, cucumber, capsicum, tomato, wheat, paddy rice, and described disease is virus disease, fusarium wilt, gray mold, anthracnose, powdery mildew, downy mildew, epidemic disease.
The selected surfactant of antibacterial volume increase composition of the present invention is known in those skilled in the art: can be selected from one or more in dispersant, wetting agent, thickener or defoamer.According to different dosage form, in preparation, can also contain stabilizing agent known in those skilled in the art, antifreeze etc.
Antibacterial volume increase composition of the present invention, can be processed on demand acceptable formulation on any agricultural chemicals, wherein preferred dosage form is wetting powder, soluble granule, soluble powder, soluble liquid, water dispersible granules, aqua or microcapsule suspending agent, can also make aqueous emulsion, microemulsion, suspending agent, effervescent agent, seed coat agent.
When making wetting powder, composition comprises following constituent content: active components A 0.01% ~ 80%, active component B0.0001% ~ 50%, dispersant 2% ~ 10%, wetting agent 2% ~ 10%, filler surplus.
When making soluble granule, composition comprises following constituent content: active components A 0.01% ~ 80%, active component B0.0001% ~ 50%, binding agent 0 ~ 8%, colouring agent 0.1%-2%, dispersant 2% ~ 10%, wetting agent 2% ~ 10%, filler surplus.
When making soluble powder, composition comprises following constituent content: active components A 0.01% ~ 80%, active component B0.0001% ~ 50%, dispersant 2% ~ 8%, wetting agent 1%-7%, stabilizing agent 0 ~ 5%, filler surplus.
When making soluble liquid, composition comprises following constituent content: active components A 0.01% ~ 80%, active component B0.0001% ~ 50%, emulsifier 1% ~ 10%, cosolvent 2% ~ 6%, solvent add to 100%.
When making water dispersible granules, composition comprises following constituent content: active components A 0.01% ~ 80%, active component B0.0001% ~ 50%, dispersant 3% ~ 12%, wetting agent 1% ~ 8%, disintegrant 1% ~ 10%, binding agent 0 ~ 8%, filler surplus.
When making aqua, composition comprises following constituent content: active components A 0.01% ~ 50%, active component B0.0001% ~ 30%, cosolvent 2% ~ 6%, wetting agent 1%-10%, antifreeze 0 ~ 8%, deionized water add to 100%.
When making microcapsule suspending agent, composition comprises following constituent content: active components A 0.01% ~ 50%, active component B0.0001% ~ 30%, macromolecule cyst material 2% ~ 10%, dispersant 1% ~ 10%, solvent 1% ~ 10%, emulsifier 1% ~ 7%, defoamer 0.01% ~ 2%, pH adjusting agent 0.01% ~ 5%, deionized water add to 100%.
Wetting powder the key technical indexes of the present invention:
Soluble granule the key technical indexes of the present invention:
Soluble powder the key technical indexes of the present invention:
Soluble liquid the key technical indexes of the present invention:
Water dispersible granules the key technical indexes of the present invention:
Aqua the key technical indexes of the present invention:
Microcapsule suspending agent the key technical indexes of the present invention:
The invention has the advantages that:
(1) Brevibacillus laterosporus and benayl aminopurine, brassin lactones, heteroauxin, triacontanol, gibberellic acid are composite has obvious promotion growth afterwards to pears, apple, grape, oranges and tangerines, banana, strawberry, watermelon, Chinese cabbage, cucumber, capsicum, tomato, wheat, paddy rice, and can prevent and treat virus disease, fusarium wilt, gray mold, anthracnose, powdery mildew, downy mildew or epidemic disease; (2) agricultural chemicals dosage reduces, and reduces the residual quantity of agricultural chemicals on crop, alleviates environmental pollution; (3) increase effective absorption of crop fertilizer, the good growth of supplemental crop inferior position part, regulates the balance of making moisture in object, thereby activates the metabolic system of crop, promotes crop cell to increase, the feature that improving activity of root system is strengthened simultaneously.
Embodiment
Below in conjunction with embodiment, to further instruction of the present invention, the percentage in embodiment is all weight percentage, but the present invention is not limited thereto.
Application Example one
Embodiment 1～15 wetting powder
Brevibacillus laterosporus, active component B, dispersant, wetting agent, filler are mixed in mixed cylinder, after airslide disintegrating mill is pulverized, mix again, can make wetting powder product of the present invention, specifically in Table 1.
Table 1 embodiment 1～15 each component and weight portion
Embodiment 16～30 soluble granules
Brevibacillus laterosporus, active component B, binding agent, colouring agent, dispersant, wetting agent, filler are mixed in mixed cylinder, at process extruder grain, dry, and make soluble granule product of the present invention through screening, specifically in Table 2.
Table 2 embodiment 1630 each component and weight portions
Embodiment 31～45 soluble powders
Brevibacillus laterosporus, active component B, dispersant, wetting agent, stabilizing agent (can add and can not add), filler are mixed in mixed cylinder, after pulverizing, airslide disintegrating mill mixes again, can make soluble powder product of the present invention, specifically in Table 3.
Table 3 embodiment 31～45 each component and weight portions
Embodiment 46～55 soluble liquids
Emulsifier, cosolvent are mixed through high speed shear, add Brevibacillus laterosporus, active component B, in ball mill, ball milling is 2 ~ 3 hours, make diameter of particle all below 5 μ m, surplus is supplied with solvent, can make soluble liquid product of the present invention, specifically in Table 4.
Table 4 embodiment 46～55 each component and weight portions
Benayl aminopurine, brassin lactones, heteroauxin, triacontanol, gibberellic acid in table 1 ~ 4 are exchanged, can make novel formulation.
Embodiment 56～58 water dispersible granules
Brevibacillus laterosporus, active component B, dispersant, wetting agent, disintegrant, filler etc. are obtained to the particle diameter needing through air-flow crushing together, then add other auxiliary agents such as binding agent (can add and can not add), obtain the materials of granulating.By item quantitatively send in fluidized bed granulation dryer through granulation and dry after, can make water dispersible granules product of the present invention, specifically in Table 5.
Table 5 embodiment 56～58 each component and content
Embodiment 59～73 aquas
Brevibacillus laterosporus, active component B, wetting agent, antifreeze (can add and can not add), solvent, deionized water etc. are mixed together, make the aqua product of the present composition, specifically in Table 6.
Table 6 embodiment 5973 each component and weight portions
Benayl aminopurine, brassin lactones, heteroauxin, triacontanol, gibberellic acid in table 6 are exchanged, can make novel formulation.
Embodiment 74,75 microcapsule suspending agents
By Brevibacillus laterosporus, active component B, macromolecule cyst material, solvent, make to be dissolved into even oil phase, under shearing condition, oil phase is joined in the aqueous phase solution that contains emulsifier, pH adjusting agent, dispersant, surplus is supplied by deionized water, bi-material reacts at oil-water interfaces, forms macromolecule cyst wall, makes the finely disseminated microcapsule suspending agent product of the present composition.Specifically in Table 7.
Table 7 embodiment 74,75 each component and weight portions
The embodiment of the present invention is the method that adopts Toxicity Determination and field trial to combine.First by Toxicity Determination, the synergy ratio (SR) after clear and definite two kinds of medicaments are composite by a certain percentage, SR < 0.5 is antagonism, 0.5≤SR≤1.5 are summation action, SR > 1.5 is synergistic effect, on this basis, then carries out field trial.
Test method: after the effective inhibition concentration scope of each medicament is determined in prerun, medicament is established respectively 5 dosage by active constituent content and processed, and establishes clear water contrast.With reference to < < farm-chemical indoor determination test rule bactericide > >, carry out, adopt mycelial growth rate method to measure the virulence of medicament to crop germ.After 72h, by right-angled intersection method, measure colony diameter, calculate and respectively process net growth, mycelial growth inhibition rate.
Net growth (mm)=measurement colony diameter-5
Mycelial growth inhibition rate is converted into probit value (y), and liquor strength (μ g/mL) converts logarithm value to (x), with method of least squares, tries to achieve virulence regression equation (y=a+bx), and calculates thus the EC of every kind of medicament
50value.According to Wadley method, calculate the different proportioning synergy ratio (SR) of two medicaments, SR < 0.5 is antagonism simultaneously, and 0.5≤SR≤1.5 are summation action, and SR > 1.5 is synergistic effect.Computing formula is as follows:
Wherein: a, b are respectively active components A and active component B shared ratio in combination;
A is Brevibacillus laterosporus;
B is selected from a kind of in benayl aminopurine, brassin lactones, heteroauxin, triacontanol, gibberellic acid.
Application Example two:
For trying disease: capsicum epidemic disease
Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..
Experimental scheme: effective inhibition concentration scope of determining the two different proportioning mixture of Brevibacillus laterosporus and benayl aminopurine through preliminary experiment.
Toxicity test result
The composite toxicity test analysis of results table to capsicum epidemic disease of table 8 Brevibacillus laterosporus and benayl aminopurine
As shown in Table 8, the proportioning of the composite control capsicum epidemic disease of Brevibacillus laterosporus and benayl aminopurine is when 10000 ︰ 1 ~ 1 ︰ 100, synergy ratio SR is all greater than 1.5, illustrate that both are mixed and all show synergistic effect in 10000 ︰ 1 ~ 1 ︰ 100 scopes, the proportioning of Brevibacillus laterosporus and benayl aminopurine was at 50: 1～1: 20, synergistic effect is more outstanding, synergy ratio is all more than 2.10, when wherein Brevibacillus laterosporus and benayl aminopurine weight ratio are 5:1, synergy ratio is maximum, and synergistic effect is the most obvious.When applicant tests the proportioning of finding Brevibacillus laterosporus and benayl aminopurine and is 50:1,40:1,30:1,20:1,15:1,10:1,9:1,8:1,7:1,6:1,5:1,4:1,3:1,2:1,1:1,1:2,1:3,1:4,1:5,1:6,1:7,1:10,1:15,1:20, the control of virus disease, fusarium wilt, gray mold, anthracnose, powdery mildew, downy mildew, epidemic disease is had to obvious synergistic effect, and synergy ratio is all more than 1.50.
Application Example three:
For trying disease: watermelon blight
Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..
Experimental scheme: effective inhibition concentration scope of determining the two different proportioning mixture of Brevibacillus laterosporus and brassin lactones through preliminary experiment.
Toxicity test result
The composite toxicity test analysis of results table to watermelon blight of table 9 Brevibacillus laterosporus and brassin lactones
As shown in Table 9, the composite control watermelon blight of Brevibacillus laterosporus and brassin lactones proportioning is when 10000 ︰ 1 ~ 1 ︰ 100, synergy ratio SR is all greater than 1.5, illustrate that both are mixed and all show synergistic effect in 10000 ︰ 1 ~ 1 ︰ 100 scopes, the proportioning of Brevibacillus laterosporus and brassin lactones was at 3000: 1～1000: 1, synergistic effect is more outstanding, synergy ratio is all more than 2.05, when wherein Brevibacillus laterosporus and brassin lactones weight ratio are 2000:1, synergy ratio is maximum, and synergistic effect is the most obvious.Through applicant, test and find that the proportioning of Brevibacillus laterosporus and brassin lactones is 3000:1, 2900:1, 2500:1, 2300:1, 2100:1, 2050:1, 2025:1, 2015:1, 2010:1, 2006:1, 2005:1, 2004:1, 2003:1, 2002:1, 2001:1, 2000:1, 1999:1, 1998:1, 1997:1, 1996:1, 1995:1, 1990:1, 1950:1, 1925:1, 1900:1, 1500:1, during 1000:1, to virus disease, fusarium wilt, gray mold, anthracnose, powdery mildew, downy mildew, the control of epidemic disease has obvious synergistic effect, synergy ratio is all more than 1.50.
Application Example four:
For trying disease: wheat scab
Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..
Experimental scheme: effective inhibition concentration scope of determining the two different proportioning mixture of Brevibacillus laterosporus and heteroauxin through preliminary experiment.
Toxicity test result
The composite toxicity test analysis of results table to wheat scab of table 10 Brevibacillus laterosporus and heteroauxin
As shown in Table 10, the composite proportioning of preventing and treating wheat scab of Brevibacillus laterosporus and heteroauxin is when 10000 ︰ 1 ~ 1 ︰ 100, synergy ratio SR is all greater than 1.5, illustrate that both are mixed and all show synergistic effect in 10000 ︰ 1 ~ 1 ︰ 100 scopes, the proportioning of Brevibacillus laterosporus and heteroauxin was at 6000: 1～3000: 1, synergistic effect is more outstanding, synergy ratio is all more than 2.15, when wherein Brevibacillus laterosporus and heteroauxin weight ratio are 5000:1, synergy ratio is maximum, and synergistic effect is the most obvious.Through applicant, test and find that the proportioning of Brevibacillus laterosporus and heteroauxin is 6000:1, 5900:1, 5500:1, 5300:1, 5100:1, 5050:1, 5025:1, 5015:1, 5010:1, 5006:1, 5005:1, 5004:1, 5003:1, 5002:1, 5001:1, 5000:1, 4999:1, 4998:1, 4997:1, 4996:1, 4995:1, 4990:1, 4950:1, 4925:1, 4000:1, 3500:1, during 3000:1, to virus disease, fusarium wilt, gray mold, anthracnose, powdery mildew, downy mildew, the control of epidemic disease has obvious synergistic effect, synergy ratio is all more than 1.50.
Application Example five:
For trying disease: grape grey mould
Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..
Experimental scheme: effective inhibition concentration scope of determining the two different proportioning mixture of Brevibacillus laterosporus and triacontanol through preliminary experiment.
Toxicity test result
The composite toxicity test analysis of results table to grape grey mould of table 11 Brevibacillus laterosporus and triacontanol
As shown in Table 11, the proportioning of the composite control grape grey mould of Brevibacillus laterosporus and triacontanol is when 10000 ︰ 1 ~ 1 ︰ 100, synergy ratio SR is all greater than 1.5, illustrate that both are mixed and all show synergistic effect in 10000 ︰ 1 ~ 1 ︰ 100 scopes, the proportioning of Brevibacillus laterosporus and triacontanol was at 600: 1～200: 1, synergistic effect is more outstanding, synergy ratio is all more than 2.15, when wherein Brevibacillus laterosporus and triacontanol weight ratio are 400:1, synergy ratio is maximum, and synergistic effect is the most obvious.When applicant tests the proportioning of finding Brevibacillus laterosporus and triacontanol and is 600:1,550:1,500:1,490:1,450:1,425:1,420:1,415:1,410:1,406:1,405:1,404:1,403:1,402:1,401:1,400:1,399:1,398:1,397:1,396:1,395:1,390:1,350:1,325:1,300:1,250:1,200:1, the control of virus disease, fusarium wilt, gray mold, anthracnose, powdery mildew, downy mildew, epidemic disease is had to obvious synergistic effect, and synergy ratio is all more than 1.50.
Application Example six:
For trying disease: citrus anthracnose
Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..
Experimental scheme: effective inhibition concentration scope of determining the two different proportioning mixture of Brevibacillus laterosporus and gibberellic acid through preliminary experiment.
Toxicity test result
The composite toxicity test analysis of results table to citrus anthracnose of table 12 Brevibacillus laterosporus and gibberellic acid
As shown in Table 12, the proportioning of the composite control citrus anthracnose of Brevibacillus laterosporus and gibberellic acid is when 10000 ︰ 1 ~ 1 ︰ 100, synergy ratio SR is all greater than 1.5, illustrate that both are mixed and all show synergistic effect in 10000 ︰ 1 ~ 1 ︰ 100 scopes, the proportioning of Brevibacillus laterosporus and gibberellic acid was at 40: 1～1: 20, synergistic effect is more outstanding, synergy ratio is all more than 2.10, when wherein Brevibacillus laterosporus and gibberellic acid weight ratio are 4:1, synergy ratio is maximum, and synergistic effect is the most obvious.When applicant tests the proportioning of finding Brevibacillus laterosporus and gibberellic acid and is 40:1,35:1,30:1,20:1,15:1,10:1,9:1,8:1,7:1,6:1,5:1,4:1,3:1,2:1,1:1,1:2,1:3,1:4,1:5,1:6,1:7,1:10,1:15,1:20, the control of virus disease, fusarium wilt, gray mold, anthracnose, powdery mildew, downy mildew, epidemic disease is had to obvious synergistic effect, and synergy ratio is all more than 1.50.
Application Example seven:
For studying thing: tomato
Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..
Experimental scheme: the concentration range of determining the two different proportioning mixture coordinate plant growth of Brevibacillus laterosporus and active component B through preliminary experiment.
The growth regulation effect of the mixed liquid of table 13 Brevibacillus laterosporus and active component B and different proportion thereof to tomato
As shown in Table 13, Brevibacillus laterosporus mixes with active component B after use, metabolic system that can activated plant and plant growth quickening vigor is strengthened, promote plant cell to increase, improving activity of root system is strengthened, reach volume increase object, Brevibacillus laterosporus and active component B, when 10000 ︰ 1 ~ 1 ︰ 100, all have stronger regulating action.
Effect experiment part: test medicine is researched and developed, provided by Shanxi Thompson Biological Technology Co., Ltd., contrast medicament 10,000,000,000 spores/gram Brevibacillus laterosporus wetting powder (autogamy), 1% benayl aminopurine soluble powder (commercial), 0.004% brassin lactones aqua (commercial), 0.11% heteroauxin aqua (commercial), 0.1% triacontanol microemulsion (commercial), 40% gibberellic acid soluble granule (commercial).
Application Example eight Brevibacillus laterosporus and active component B and composite control tomato virus disease drug effect and growth regulation Experiment on Function
This experimental establishment, in Xi'an City, Shanxi Province, is investigated the tomato virus disease state of an illness before medicine, in the dispenser for the first time of state of an illness initial stage, once, dispenser is 2 times altogether in dispenser in every 7 days.Within after dispenser 3 days, 7 days, 15 days for the second time, investigate respectively disease index and calculate preventive effect, for the second time the growth rate of dispenser " Invest, Then Investigate " crop.Experimental result is as follows:
Table 14 Brevibacillus laterosporus and active component B and composite control tomato virus disease drug effect and growth regulation Experiment on Function
As can be seen from Table 14, Brevibacillus laterosporus and active component B and composite after can effectively prevent and treat tomato virus disease, control efficiency is all better than the preventive effect of single dose, the preventive effect phase is long, and metabolic system that can activated plant and plant growth quickening vigor is strengthened, strengthens the growth of root system.In test scope of medication, target crop is had no adverse effects simultaneously.
Application Example nine Brevibacillus laterosporus and active component B and composite wheat scab drug effect and the growth regulation Experiment on Function prevented and treated thereof
This test arrangement, in Weinan City Pucheng County, Shaanxi Province, is investigated the wheat scab state of an illness before medicine, in the dispenser for the first time of state of an illness initial stage, once, dispenser is 2 times altogether in dispenser in every 7 days.Within after dispenser 3 days, 7 days, 15 days for the second time, investigate respectively disease index and calculate preventive effect, for the second time the growth rate of dispenser " Invest, Then Investigate " crop.Experimental result is as follows:
Table 15 Brevibacillus laterosporus and active component B and composite wheat scab drug effect and the growth regulation Experiment on Function prevented and treated thereof
As can be seen from Table 15, Brevibacillus laterosporus and active component B and composite after can effectively prevent and treat wheat scab, control efficiency is all better than the preventive effect of single dose, the preventive effect phase is long, and metabolic system that can activated plant and plant growth quickening vigor is strengthened, strengthens the growth of root system.In test scope of medication, target crop is had no adverse effects simultaneously.
Application Example ten Brevibacillus laterosporus and active component B and composite control watermelon blight drug effect and growth regulation Experiment on Function
This experimental establishment, at Xian District, Shanxi Province suburban area, is investigated the watermelon blight state of an illness before medicine, in the dispenser for the first time of state of an illness initial stage, once, dispenser is 2 times altogether in dispenser in every 7 days.Within after dispenser 7 days, 14 days, 30 days for the second time, investigate respectively disease index and calculate preventive effect, for the second time the growth rate of dispenser " Invest, Then Investigate " crop.Experimental result is as follows:
Table 16 Brevibacillus laterosporus and active component B and composite control watermelon blight drug effect and growth regulation Experiment on Function
As can be seen from Table 16, Brevibacillus laterosporus and active component B and composite after can effectively prevent and treat watermelon blight, control efficiency is all better than the preventive effect of single dose, the preventive effect phase is long, and metabolic system that can activated plant and plant growth quickening vigor is strengthened, strengthens the growth of root system.In test scope of medication, target crop is had no adverse effects simultaneously.
Application Example 11 Brevibacillus laterosporus and active component B and composite control grape grey mould drug effect and growth regulation Experiment on Function
This experimental establishment, in Weinan City Dali County, Shaanxi Province, is investigated the grape grey mould state of an illness before medicine, in the dispenser for the first time of state of an illness initial stage, once, dispenser is 2 times altogether in dispenser in every 7 days.Within after dispenser 3 days, 7 days, 15 days for the second time, investigate respectively disease index and calculate preventive effect, for the second time the growth rate of dispenser " Invest, Then Investigate " crop.Experimental result is as follows:
Table 17 Brevibacillus laterosporus and active component B and composite control grape grey mould drug effect and growth regulation Experiment on Function
As can be seen from Table 17, Brevibacillus laterosporus and active component B and composite after can effectively prevent and treat grape grey mould, control efficiency is all better than the preventive effect of single dose, the preventive effect phase is long, and metabolic system that can activated plant and plant growth quickening vigor is strengthened, strengthens the growth of root system.In test scope of medication, target crop is had no adverse effects simultaneously.
Application Example 12 Brevibacillus laterosporus and active component B and composite control citrus anthracnose drug effect and growth regulation Experiment on Function
This experimental establishment, at Shaanxi Province's Hanzhong City, is investigated the citrus anthracnose state of an illness before medicine, in the dispenser for the first time of state of an illness initial stage, once, dispenser is 2 times altogether in dispenser in every 7 days.Within after dispenser 7 days, 14 days, 30 days for the second time, investigate respectively disease index and calculate preventive effect, for the second time the growth rate of dispenser " Invest, Then Investigate " crop.Experimental result is as follows:
Table 18 Brevibacillus laterosporus and active component B and composite control citrus anthracnose drug effect and growth regulation Experiment on Function
As can be seen from Table 18, Brevibacillus laterosporus and active component B and composite after can effectively prevent and treat citrus anthracnose, control efficiency is all better than the preventive effect of single dose, the preventive effect phase is long, and metabolic system that can activated plant and plant growth quickening vigor is strengthened, strengthens the growth of root system.In test scope of medication, target crop is had no adverse effects simultaneously.
Application Example 13 Brevibacillus laterosporus and active component B and composite powdery mildew of cucumber drug effect and the growth regulation Experiment on Function prevented and treated thereof
This experimental establishment, in Jingyang County, Shaanxi Province, is investigated the powdery mildew of cucumber state of an illness before medicine, in the dispenser for the first time of state of an illness initial stage, once, dispenser is 2 times altogether in dispenser in every 7 days.Within after dispenser 3 days, 7 days, 15 days for the second time, investigate respectively disease index and calculate preventive effect, for the second time the growth rate of dispenser " Invest, Then Investigate " crop.Experimental result is as follows:
Table 19 Brevibacillus laterosporus and active component B and composite powdery mildew of cucumber drug effect and the growth regulation Experiment on Function prevented and treated thereof
As can be seen from Table 19, Brevibacillus laterosporus and active component B and composite after can effectively prevent and treat powdery mildew of cucumber, control efficiency is all better than the preventive effect of single dose, the preventive effect phase is long, and metabolic system that can activated plant and plant growth quickening vigor is strengthened, strengthens the growth of root system.In test scope of medication, target crop is had no adverse effects simultaneously.
After through different local throughout the country tests, draw, Brevibacillus laterosporus and active component B composite rear to the preventive effect of the Common Diseases such as the virus disease in various crop, fusarium wilt, gray mold, anthracnose, powdery mildew, downy mildew or epidemic disease all 95% with on excellent, in single dose preventive effect, synergistic effect is obvious.Metabolic system that simultaneously can activated plant and plant growth quickening vigor is strengthened, the increase of proline content and the reinforcement of cellulase, promote plant cell to increase, improving activity of root system is strengthened, and reaches volume increase object.
Claims (8)
1. the antibacterial volume increase composition containing Brevibacillus laterosporus, effective active composition is active components A and active component B, it is characterized in that: the weight ratio of active components A and active component B is 10000:1 ~ 1:100, described active components A is selected from Brevibacillus laterosporus, and active component B is selected from a kind of in benayl aminopurine, brassin lactones, heteroauxin, triacontanol or gibberellic acid.
2. the antibacterial volume increase composition containing Brevibacillus laterosporus according to claim 1, is characterized in that: in composition, the weight ratio of active components A and active component B is 8000:1 ~ 1:50.
3. the antibacterial volume increase composition containing Brevibacillus laterosporus according to claim 2, is characterized in that:
The weight ratio of Brevibacillus laterosporus and benayl aminopurine is 50:1 ~ 1:20;
The weight ratio of Brevibacillus laterosporus and brassin lactones is 3000:1 ~ 1000:1;
The weight ratio of Brevibacillus laterosporus and heteroauxin is 6000:1 ~ 3000:1;
The weight ratio of Brevibacillus laterosporus and triacontanol is 600:1 ~ 200:1;
The weight ratio of Brevibacillus laterosporus and gibberellic acid is 40:1 ~ 1:20.
4. the antibacterial volume increase composition containing Brevibacillus laterosporus according to claim 1, is characterized in that: wherein effective active component content accounts for 0.0001%～60% of gross weight.
5. the antibacterial volume increase composition containing Brevibacillus laterosporus according to claim 4, is characterized in that: wherein effective active component content accounts for 0.001%～20% of gross weight.
6. according to the antibacterial volume increase composition containing Brevibacillus laterosporus described in any one in claim 1 to 5, it is characterized in that: composition is made wetting powder, soluble granule, soluble powder, soluble liquid, water dispersible granules, aqua or microcapsule suspending agent.
7. the antibacterial volume increase composition containing Brevibacillus laterosporus according to claim 1 is used for the purposes of preventing and treating crop pest and Promoting plant growth.
8. purposes according to claim 7, is characterized in that: described disease is virus disease, fusarium wilt, gray mold, anthracnose, powdery mildew, downy mildew or epidemic disease.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105557689A (en) * | 2015-12-19 | 2016-05-11 | 佛山市艳晖生物科技有限公司 | Bacillus wettable powder containing brassinolide and preparation method thereof |
| CN105580843A (en) * | 2015-12-19 | 2016-05-18 | 佛山市艳晖生物科技有限公司 | Brassinolide containing spore pesticide fertilizer and preparation method thereof |
| CN105594698A (en) * | 2015-12-19 | 2016-05-25 | 佛山市艳晖生物科技有限公司 | Bacillus laterosporus wettable powder preparation method |
| CN109805007A (en) * | 2017-11-22 | 2019-05-28 | 河南福瑞得生物科技有限公司 | A kind of bacillus subtilis wettable powder |
| CN112913843A (en) * | 2021-01-27 | 2021-06-08 | 内蒙古大学 | Control effect of indoleacetic acid on 4 plant disease fungi |
| CN115349519A (en) * | 2022-08-30 | 2022-11-18 | 山东大成生物化工有限公司 | Brassinolide-gibberellic acid-bacillus aryabhattai compound pesticide fertilizer and preparation method and application thereof |
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- 2013-01-10 CN CN201310009085.5A patent/CN103918715A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105557689A (en) * | 2015-12-19 | 2016-05-11 | 佛山市艳晖生物科技有限公司 | Bacillus wettable powder containing brassinolide and preparation method thereof |
| CN105580843A (en) * | 2015-12-19 | 2016-05-18 | 佛山市艳晖生物科技有限公司 | Brassinolide containing spore pesticide fertilizer and preparation method thereof |
| CN105594698A (en) * | 2015-12-19 | 2016-05-25 | 佛山市艳晖生物科技有限公司 | Bacillus laterosporus wettable powder preparation method |
| CN105594698B (en) * | 2015-12-19 | 2018-06-01 | 佛山市艳晖生物科技有限公司 | A kind of preparation method of bacillus laterosporus wettable powder |
| CN105580843B (en) * | 2015-12-19 | 2018-09-28 | 佛山市艳晖生物科技有限公司 | A kind of gemma medicine fertilizer and preparation method thereof containing brassin lactones |
| CN105557689B (en) * | 2015-12-19 | 2018-09-28 | 佛山市艳晖生物科技有限公司 | A kind of bacillus wettable powder and preparation method thereof containing brassin lactones |
| CN109805007A (en) * | 2017-11-22 | 2019-05-28 | 河南福瑞得生物科技有限公司 | A kind of bacillus subtilis wettable powder |
| CN112913843A (en) * | 2021-01-27 | 2021-06-08 | 内蒙古大学 | Control effect of indoleacetic acid on 4 plant disease fungi |
| CN115349519A (en) * | 2022-08-30 | 2022-11-18 | 山东大成生物化工有限公司 | Brassinolide-gibberellic acid-bacillus aryabhattai compound pesticide fertilizer and preparation method and application thereof |
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Application publication date: 20140716 |