CN103918719A - High-efficiency sterilization composition containing brevibacillus laterosporus - Google Patents
High-efficiency sterilization composition containing brevibacillus laterosporus Download PDFInfo
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Abstract
The invention discloses a high-efficiency sterilization composition containing brevibacillus laterosporus. Effective active components of the sterilization composition comprise an active component A and an active component B, wherein the active component A is selected from brevibacillus laterosporus; the active component B is selected from any one of the following compounds: azoxystrobin, kresoxim-methyl, pyraclostrobin, trifloxystrobin, fluoxastrobin, dimoxystrobin, orysastrobin, metominostrobin, pyraoxystrobin, benzene kresoxim-methyl, and picoxystrobin; the weight percentage ratio of the active component A to the active component B is 0.1%-20%:0.5%-80%. The composition has higher activity against various diseases on various crops, has an obvious synergistic effect, expands a sterilization spectrum, besides, increases effective absorption of crops on fertilizers, assists a crop disadvantage part to well grow, adjusts the balance of water in the crops, thereby activating a crop metabolic system, promoting crop cell growth, and enhancing root system vitality.
Description
Technical field
The invention belongs to technical field of pesticide, relate to the application of a kind of efficient bactericidal composite containing Brevibacillus laterosporus on corps diseases.
Background technology
Brevibacillus laterosporus is new type bactericide, can be used for preventing and treating multiple diseases, for example capsicum epidemic disease, watermelon blight, wheat scab, grape grey mould, Strawberry anthracnose.
Fluoxastrobin, kresoxim-methyl, pyraclostrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, orysastrobin, SSF 126, azoles bacterium ester, ZJ0712 or ZEN 90160 all belong to methoxy acrylic bactericide, are widely used in the disease of crops.
But in the real process of agricultural production, preventing and treating the problem that disease the most easily produces is the generation of disease drug resistance.Different cultivars composition carries out composite, is to prevent and treat the very common method of resistance disease.Heterogeneity is carried out composite, according to practical application effect, judge certain composite be synergy, add and or antagonism.In most cases, the composite effect of sterilization is all additive effect, really have a synergistic effect composite seldom, especially synergistic effect very obviously, synergy ratio very high composite just still less.Through inventor's research, find Brevibacillus laterosporus and Fluoxastrobin, kresoxim-methyl, pyraclostrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, orysastrobin, SSF 126, azoles bacterium ester, ZJ0712 or ZEN 90160 can produce good synergistic effect after composite, and not yet open about Brevibacillus laterosporus and the composite relevant report of Fluoxastrobin, kresoxim-methyl, pyraclostrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, orysastrobin, SSF 126, azoles bacterium ester, ZJ0712 or ZEN 90160.
Summary of the invention
The object of the invention is to propose a kind of have synergistic function, use cost is low, preventive effect the is good efficient bactericidal composite containing Brevibacillus laterosporus.
The efficient bactericidal composite containing Brevibacillus laterosporus that the present invention proposes contains active components A and active component B, active components A and active component B percentage by weight are 0.1% ~ 20%:0.5% ~ 80%, described active components A is selected from Brevibacillus laterosporus, active component B is selected from the one in Fluoxastrobin, kresoxim-methyl, pyraclostrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, orysastrobin, SSF 126, azoles bacterium ester, ZJ0712 or ZEN 90160, and active components A and the preferred percentage by weight of active component B are 0.5% ~ 15%:1% ~ 60%; More preferably the percentage by weight of Brevibacillus laterosporus and Fluoxastrobin or SSF 126 or ZEN 90160 is 1% ~ 10%:5% ~ 60%, the percentage by weight of Brevibacillus laterosporus and pyraclostrobin or kresoxim-methyl or oxime bacterium ester or dimoxystrobin or orysastrobin or fluoxastrobin is 1% ~ 10%:5% ~ 50%, and the percentage by weight of Brevibacillus laterosporus and azoles bacterium ester or ZJ0712 is 1% ~ 10%:2% ~ 45%.
The efficient bactericidal composite containing Brevibacillus laterosporus that the present invention proposes is for preventing and treating the purposes of disease on crops, and described crops comprise cereal crops, legume crop, fiber crop, sugar [yielding, melon crop, fruits crop, dry fruit crop, hobby crop, root crop, oil crop, flowers crop, medicinal crop, raw material crop, green manure pasture crop; Described disease comprises powdery mildew, downy mildew, virus disease, head blight, fusarium wilt, gray mold, epidemic disease, leaf mold, shot hole, scab, peronospora tabacina, anthracnose, spot defoliation, rust, rice blast, false smut, banded sclerotial blight, brown spot or leaf blight.
The percentage by weight of active components A, active component B is 0.1% ~ 20%:0.5% ~ 80%.Conventionally in composition, the weight percentage of active component is gross weight 0.5%～90%, is preferably 5%～80%.According to different preparation types, active component content scope is different.Conventionally, liquid preparation contains 1%～70% active substance by weight, is preferably 5%～50%; Solid pharmaceutical preparation contains 5%～80% active substance by weight, is preferably 10%～80%.
In bactericidal composition of the present invention, at least contain a kind of surfactant, the dispersion of active component in water while being beneficial to use.Surface-active contents is 5%～30% of total formulation weight amount, and surplus is solid or liquid diluent.
Bactericidal composition of the present invention can be by user before use through dilution or directly use.Its preparation can be prepared by common processing method known in those skilled in the art, after mixing with liquid flux or solid carrier by active substance, then add surfactant as one or more in dispersant, stabilizing agent, wetting agent, binding agent, defoamer, disintegrant, antifreeze etc.
Bactericidal composition of the present invention, can be processed on demand acceptable formulation in any sterilization, wherein more preferably formulation is wetting powder, water dispersible granules, suspending agent, aqueous emulsion, microemulsion, microcapsule suspending agent, can also make suspension emulsion, microcapsule suspension-suspending agent.
When making wetting powder, composition comprises following constituent content: active components A 0.1% ~ 20%, active component B0.5% ~ 80%, dispersant 2% ~ 10%, wetting agent 2% ~ 10%, filler surplus.
When making water dispersible granules, composition comprises following constituent content: active components A 0.1% ~ 20%, active component B0.5% ~ 80%, dispersant 3% ~ 12%, wetting agent 1% ~ 8%, disintegrant 1% ~ 10%, binding agent 0 ~ 8%, filler surplus.
When making suspending agent, composition comprises following constituent content: active components A 0.1% ~ 20%, active component B0.5% ~ 50%, dispersant 2% ~ 10%, wetting agent 2% ~ 10%, defoamer 0.01% ~ 2%, thickener 0 ~ 2%, antifreeze 0 ~ 8%, deionized water add to 100%.
When making aqueous emulsion, composition comprises following constituent content: active components A 0.1% ~ 20%, active component B0.5% ~ 50%, solvent 1% ~ 30%, emulsifier 1% ~ 15%, antifreeze 0 ~ 8%, thickener 0 ~ 2%, defoamer 0.01% ~ 2%, deionized water are supplied surplus.
When making microemulsion, composition comprises following constituent content: active components A 0.1% ~ 20%, active component B0.5% ~ 50%, solvent 1% ~ 30%, emulsifier 3% ~ 25%, antifreeze 0 ~ 8%, defoamer 0.01% ~ 2%, deionized water are supplied surplus.
When making microcapsule suspending agent, composition comprises following constituent content: active components A 0.1% ~ 20%, active component B0.5% ~ 50%, macromolecule cyst material 2% ~ 10%, dispersant 1% ~ 10%, organic solvent 1% ~ 10%, emulsifier 1% ~ 7%, defoamer 0.01% ~ 2%, pH adjusting agent 0.01% ~ 5%, deionized water add to 100%.
Wetting powder the key technical indexes of the present invention:
Water dispersible granules the key technical indexes of the present invention:
Suspending agent the key technical indexes of the present invention:
Aqueous emulsion the key technical indexes of the present invention:
Microemulsion the key technical indexes of the present invention:
Microcapsule suspending agent the key technical indexes of the present invention:
The invention has the advantages that:
(1) Brevibacillus laterosporus and Fluoxastrobin, kresoxim-methyl, pyraclostrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, orysastrobin, SSF 126, azoles bacterium ester, ZJ0712 or ZEN 90160 composite after, there is obvious synergy and lasting effect; (2) disease on cereal crops, legume crop, fiber crop, sugar [yielding, melon crop, fruits crop, dry fruit crop, hobby crop, root crop, oil crop, flowers crop, medicinal crop, raw material crop, green manure pasture crop is all had to greater activity; (3) reduce agricultural chemicals dosage, reduced the residual quantity of agricultural chemicals on crop, alleviated environmental pollution; (4), to person poultry safety, Environmental compatibility is good; And preparation adhesion strength strengthens, resistance of rainwater washing against.
Embodiment
Below in conjunction with embodiment, to further instruction of the present invention, the percentage in embodiment is all weight percentage, but the present invention is not limited thereto.
Application Example one
Embodiment 1～26 wetting powder
Brevibacillus laterosporus, active component B, dispersant, wetting agent, filler are mixed in mixed cylinder, after airslide disintegrating mill is pulverized, mix again, can make wetting powder product of the present invention, specifically in table 1.
The each component of table 1 embodiment 1～26 and weight portion
Embodiment 27～52 water dispersible granules
Brevibacillus laterosporus, active component B, dispersant, wetting agent, disintegrant, filler etc. are obtained to the particle diameter needing through air-flow crushing together, then add other auxiliary agents such as binding agent (can add and can not add), obtain the materials of granulating.By item quantitatively send in fluidized bed granulation dryer through granulation and dry after, can make water dispersible granules product of the present invention, specifically in table 2.
The each component of table 2 embodiment 27～52 and weight portion
Embodiment 53～78 suspending agents
By dispersant, wetting agent, defoamer, thickener (can add and can not add), antifreeze (can add and can not add), mix through high speed shear, add Brevibacillus laterosporus, active component B, ball milling 2 ~ 3 hours in ball mill, make diameter of particle all below 5 μ m, surplus is supplied by deionized water, can make suspending agent product of the present invention, specifically in table 3.
The each component of table 3 embodiment 53～78 and weight portion
Fluoxastrobin, kresoxim-methyl, pyraclostrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, orysastrobin, SSF 126, azoles bacterium ester, ZJ0712, ZEN 90160 in table 1 ~ 3 are exchanged, can make novel formulation.
Embodiment 79～83 aqueous emulsions
Brevibacillus laterosporus, active component B, solvent, emulsifier are added together, make to be dissolved into even oil phase; Deionized water, antifreeze (can add and can not add), thickener (can add and can not add), defoamer are mixed, become homogeneous water.Under high-speed stirred, water is added to oil phase, surplus is supplied by deionized water; Can make aqueous emulsion product of the present invention, specifically in table 4.
The each component of table 4 embodiment 79～83 and weight portion
Embodiment 84～86 microemulsions
Brevibacillus laterosporus, active component B, solvent, emulsifier, antifreeze (can add and can not add), defoamer are fully mixed into the oil phase of homogeneous transparent, under agitation slowly add deionized water, form WO emulsion, again through agitating heating, make it rapid phase inversion and become oil-in-water type, be chilled to room temperature and make it to reach balance, after filtration, surplus is supplied by deionized water; Can make microemulsion product of the present invention, specifically in table 5.
The each component of table 5 embodiment 84～86 and weight portion
Embodiment 87,88 microcapsule suspending agents
By Brevibacillus laterosporus, active component B, macromolecule cyst material, solvent, make to be dissolved into even oil phase, under shearing condition, oil phase is joined in the aqueous phase solution that contains emulsifier, pH adjusting agent, dispersant, surplus is supplied by deionized water, bi-material reacts at oil-water interfaces, forms macromolecule cyst wall, makes the finely disseminated microcapsule suspending agent product of the present composition.Specifically in table 6.
Table 6 embodiment 87,88 each component and weight portions
The embodiment of the present invention is the method that adopts Toxicity Determination and field trial to combine.First by Toxicity Determination, the synergy ratio (SR) after clear and definite two kinds of medicaments are composite by a certain percentage, SR < 0.5 is antagonism, 0.5≤SR≤1.5 are summation action, SR > 1.5 is synergistic effect, on this basis, then carries out field trial.
Test method: after the effective inhibition concentration scope of each medicament is determined in prerun, medicament is established respectively 5 dosage processing by active constituent content, establishes clear water contrast.Carry out with reference to " farm-chemical indoor determination test rule bactericide ", adopt mycelial growth rate method to measure the virulence of medicament to crop germ.After 72h, measure colony diameter by right-angled intersection method, calculate each processing net growth, mycelial growth inhibition rate.
Net growth (mm)=measurement colony diameter-5
Mycelial growth inhibition rate is converted into probit value (y), and liquor strength (μ g/mL) converts logarithm value to (x), tries to achieve virulence regression equation (y=a+bx) with method of least squares, and calculates thus the EC of every kind of medicament
50value.Calculate the different proportioning synergy ratios of two medicaments (SR) according to Wadley method, SR < 0.5 is antagonism simultaneously, and 0.5≤SR≤1.5 are summation action, and SR > 1.5 is synergistic effect.Computing formula is as follows:
Wherein: a, b are respectively active components A and active component B shared ratio in combination;
A is Brevibacillus laterosporus;
B is selected from the one in Fluoxastrobin, kresoxim-methyl, pyraclostrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, orysastrobin, SSF 126, azoles bacterium ester, ZJ0712, ZEN 90160.
Application Example two:
For trying disease: powdery mildew of cucumber
Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..
Experimental scheme: effective inhibition concentration scope of determining the former medicine of Brevibacillus laterosporus and Fluoxastrobin and the two different proportioning mixture through preliminary experiment.
Toxicity test result
Table 7 Brevibacillus laterosporus and the azoxystrobin compound toxicity test analysis of results table to powdery mildew of cucumber
As shown in Table 7, Brevibacillus laterosporus and the Fluoxastrobin proportioning synergy ratio SR to powdery mildew of cucumber in the time of 1 ︰ 800 ~ 40 ︰ 1 is all greater than 1.5, illustrate that both all show synergistic effect at mixture in 1 ︰ 800 ~ 40 ︰ 1 scopes, when the proportioning of Brevibacillus laterosporus and Fluoxastrobin is during at 1 ︰ 60 ~ 2 ︰ 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.15.Test and find that the optimum ratio of Brevibacillus laterosporus and Fluoxastrobin is 2:1,1:1,1:2,1:3,1:4,1:5,1:6,1:7,1:8,1:9,1:10,1:11,1:12,1:13,1:14,1:15,1:16,1:17,1:18,1:19,1:20,1:30,1:40,1:60 through applicant, especially synergy ratio maximum in the time that Brevibacillus laterosporus and Fluoxastrobin weight ratio are 1:11, synergistic effect is the most obvious.
Application Example three
For trying disease: grape powdery mildew
Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..
Experimental scheme: effective inhibition concentration scope of determining the former medicine of Brevibacillus laterosporus and kresoxim-methyl and the two different proportioning mixture through preliminary experiment.
The composite toxicity test analysis of results table to grape powdery mildew of table 8 Brevibacillus laterosporus and kresoxim-methyl
As shown in Table 8, Brevibacillus laterosporus and the kresoxim-methyl proportioning synergy ratio SR to grape powdery mildew in the time of 1 ︰ 800 ~ 40 ︰ 1 is all greater than 1.5, illustrate that both all show synergistic effect at mixture in 1 ︰ 800 ~ 40 ︰ 1 scopes, when the proportioning of Brevibacillus laterosporus and kresoxim-methyl is during at 1 ︰ 50 ~ 2 ︰ 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.15.Test and find that the optimum ratio of Brevibacillus laterosporus and kresoxim-methyl is 2:1,1:1,1:2,1:3,1:4,1:5,1:6,1:7,1:8,1:9,1:10,1:11,1:12,1:13,1:14,1:15,1:16,1:17,1:18,1:19,1:20,1:30,1:40,1:50 through applicant, especially synergy ratio maximum in the time that Brevibacillus laterosporus and kresoxim-methyl weight ratio are 1:9, synergistic effect is the most obvious.
Application Example four
For trying disease: grape powdery mildew
Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..
Experimental scheme: effective inhibition concentration scope of determining the former medicine of Brevibacillus laterosporus and pyraclostrobin and the two different proportioning mixture through preliminary experiment.
The composite toxicity test analysis of results table to grape powdery mildew of table 9 Brevibacillus laterosporus and pyraclostrobin
As shown in Table 9, Brevibacillus laterosporus and the pyraclostrobin proportioning synergy ratio SR to grape powdery mildew in the time of 1 ︰ 800 ~ 40 ︰ 1 is all greater than 1.5, illustrate that both all show synergistic effect at mixture in 1 ︰ 800 ~ 40 ︰ 1 scopes, when the proportioning of Brevibacillus laterosporus and pyraclostrobin is during at 1 ︰ 50 ~ 2 ︰ 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.15.Test and find that the optimum ratio of Brevibacillus laterosporus and pyraclostrobin is 2:1,1:1,1:2,1:3,1:4,1:5,1:6,1:7,1:8,1:9,1:10,1:11,1:12,1:13,1:14,1:15,1:16,1:17,1:18,1:19,1:20,1:30,1:40,1:50 through applicant, especially synergy ratio maximum in the time that Brevibacillus laterosporus and pyraclostrobin weight ratio are 1:9, synergistic effect is the most obvious.
Application Example five
For trying disease: downy mildew of garpe
Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..
Experimental scheme: effective inhibition concentration scope of determining Brevibacillus laterosporus and the former medicine of oxime bacterium ester and the two different proportioning mixture through preliminary experiment.
The composite toxicity test analysis of results table to downy mildew of garpe of table 10 Brevibacillus laterosporus and oxime bacterium ester
As shown in Table 10, Brevibacillus laterosporus and the oxime bacterium ester proportioning synergy ratio SR to downy mildew of garpe in the time of 1 ︰ 800 ~ 40 ︰ 1 is all greater than 1.5, illustrate that both all show synergistic effect at mixture in 1 ︰ 800 ~ 40 ︰ 1 scopes, when the proportioning of Brevibacillus laterosporus and oxime bacterium ester is during at 1 ︰ 50 ~ 2 ︰ 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.10.Test and find that the optimum ratio of Brevibacillus laterosporus and oxime bacterium ester is 2:1,1:1,1:2,1:3,1:4,1:5,1:6,1:7,1:8,1:9,1:10,1:11,1:12,1:13,1:14,1:15,1:16,1:17,1:18,1:19,1:20,1:30,1:40,1:50 through applicant, especially synergy ratio maximum in the time that Brevibacillus laterosporus and oxime bacterium ester weight ratio are 1:9, synergistic effect is the most obvious.
Application Example six
For trying disease: wheat rust
Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..
Experimental scheme: effective inhibition concentration scope of determining the former medicine of Brevibacillus laterosporus and fluoxastrobin and the two different proportioning mixture through preliminary experiment.
The composite toxicity test analysis of results table to wheat rust of table 11 Brevibacillus laterosporus and fluoxastrobin
As shown in Table 11, Brevibacillus laterosporus and the fluoxastrobin proportioning synergy ratio SR to wheat rust in the time of 1 ︰ 800 ~ 40 ︰ 1 is all greater than 1.5, illustrate that both all show synergistic effect at mixture in 1 ︰ 800 ~ 40 ︰ 1 scopes, when the proportioning of Brevibacillus laterosporus and fluoxastrobin is during at 1 ︰ 50 ~ 2 ︰ 1, synergistic effect is more outstanding, synergy ratio all more than 2.20, synergy ratio maximum in the time that Brevibacillus laterosporus and fluoxastrobin weight ratio are 1:9, synergistic effect is the most obvious.
Application Example seven
For trying disease: downy mildew of garpe
Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..
Experimental scheme: effective inhibition concentration scope of determining the former medicine of Brevibacillus laterosporus and dimoxystrobin and the two different proportioning mixture through preliminary experiment.
The composite toxicity test analysis of results table to downy mildew of garpe of table 12 Brevibacillus laterosporus and dimoxystrobin
As shown in Table 12, Brevibacillus laterosporus and the dimoxystrobin proportioning synergy ratio SR to downy mildew of garpe in the time of 1 ︰ 800 ~ 40 ︰ 1 is all greater than 1.5, illustrate that both all show synergistic effect at mixture in 1 ︰ 800 ~ 40 ︰ 1 scopes, when the proportioning of Brevibacillus laterosporus and dimoxystrobin is during at 1 ︰ 50 ~ 2 ︰ 1, synergistic effect is more outstanding, synergy ratio all more than 2.10, synergy ratio maximum in the time that Brevibacillus laterosporus and dimoxystrobin weight ratio are 1:9, synergistic effect is the most obvious.Application Example eight
For trying disease: rice sheath blight disease
Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..
Experimental scheme: effective inhibition concentration scope of determining the former medicine of Brevibacillus laterosporus and orysastrobin and the two different proportioning mixture through preliminary experiment.
The composite toxicity test analysis of results table to rice sheath blight disease of table 13 Brevibacillus laterosporus and orysastrobin
As shown in Table 13, Brevibacillus laterosporus and the orysastrobin proportioning synergy ratio SR to rice sheath blight disease in the time of 1 ︰ 800 ~ 40 ︰ 1 is all greater than 1.5, illustrate that both all show synergistic effect at mixture in 1 ︰ 800 ~ 40 ︰ 1 scopes, when the proportioning of Brevibacillus laterosporus and orysastrobin is during at 1 ︰ 50 ~ 2 ︰ 1, synergistic effect is more outstanding, synergy ratio all more than 2.20, synergy ratio maximum in the time that Brevibacillus laterosporus and orysastrobin weight ratio are 1:9, synergistic effect is the most obvious.
Application Example nine
For trying disease: rice blast
Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..
Experimental scheme: effective inhibition concentration scope of determining the former medicine of Brevibacillus laterosporus and SSF 126 and the two different proportioning mixture through preliminary experiment.
The composite toxicity test analysis of results table to rice blast of table 14 Brevibacillus laterosporus and SSF 126
As shown in Table 14, Brevibacillus laterosporus and the SSF 126 proportioning synergy ratio SR to rice blast in the time of 1 ︰ 800 ~ 40 ︰ 1 is all greater than 1.5, illustrate that both all show synergistic effect at mixture in 1 ︰ 800 ~ 40 ︰ 1 scopes, when the proportioning of Brevibacillus laterosporus and SSF 126 is during at 1 ︰ 60 ~ 2 ︰ 1, synergistic effect is more outstanding, synergy ratio all more than 2.20, synergy ratio maximum in the time that Brevibacillus laterosporus and SSF 126 weight ratio are 1:11, synergistic effect is the most obvious.
Application Example ten
For trying disease: cucumber downy mildew
Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..
Experimental scheme: effective inhibition concentration scope of determining Brevibacillus laterosporus and the former medicine of azoles bacterium ester and the two different proportioning mixture through preliminary experiment.
The composite toxicity test analysis of results table to cucumber downy mildew of table 15 Brevibacillus laterosporus and azoles bacterium ester
As shown in Table 15, Brevibacillus laterosporus and the azoles bacterium ester proportioning synergy ratio SR to cucumber downy mildew in the time of 1 ︰ 800 ~ 40 ︰ 1 is all greater than 1.5, illustrate that both all show synergistic effect at mixture in 1 ︰ 800 ~ 40 ︰ 1 scopes, when the proportioning of Brevibacillus laterosporus and azoles bacterium ester is during at 1 ︰ 45 ~ 5 ︰ 1, synergistic effect is more outstanding, synergy ratio all more than 2.15, synergy ratio maximum in the time that Brevibacillus laterosporus and azoles bacterium ester weight ratio are 1:3, synergistic effect is the most obvious.
Application Example 11
For trying disease: powdery mildew of cucumber
Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..
Experimental scheme: effective inhibition concentration scope of determining the former medicine of Brevibacillus laterosporus and ZJ0712 and the two different proportioning mixture through preliminary experiment.
The composite toxicity test analysis of results table to powdery mildew of cucumber of table 16 Brevibacillus laterosporus and ZJ0712
As shown in Table 16, Brevibacillus laterosporus and the ZJ0712 proportioning synergy ratio SR to powdery mildew of cucumber in the time of 1 ︰ 800 ~ 40 ︰ 1 is all greater than 1.5, illustrate that both all show synergistic effect at mixture in 1 ︰ 800 ~ 40 ︰ 1 scopes, when the proportioning of Brevibacillus laterosporus and ZJ0712 is during at 1 ︰ 45 ~ 5 ︰ 1, synergistic effect is more outstanding, synergy ratio all more than 2.05, synergy ratio maximum in the time that Brevibacillus laterosporus and ZJ0712 weight ratio are 1:3, synergistic effect is the most obvious.
Application Example 12
For trying disease: wheat leaf blight
Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..
Experimental scheme: effective inhibition concentration scope of determining the former medicine of Brevibacillus laterosporus and ZEN 90160 and the two different proportioning mixture through preliminary experiment.
The composite toxicity test analysis of results table to wheat leaf blight of table 17 Brevibacillus laterosporus and ZEN 90160
As shown in Table 17, Brevibacillus laterosporus and the ZEN 90160 proportioning synergy ratio SR to wheat leaf blight in the time of 1 ︰ 800 ~ 40 ︰ 1 is all greater than 1.5, illustrate that both all show synergistic effect at mixture in 1 ︰ 800 ~ 40 ︰ 1 scopes, when the proportioning of Brevibacillus laterosporus and ZEN 90160 is during at 1 ︰ 60 ~ 2 ︰ 1, synergistic effect is more outstanding, synergy ratio all more than 2.20, synergy ratio maximum in the time that Brevibacillus laterosporus and ZEN 90160 weight ratio are 1:11, synergistic effect is the most obvious.
Application Example 13
For studying thing: tomato
Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..
Experimental scheme: the concentration range of determining the two different proportioning mixture coordinate plant growth of Brevibacillus laterosporus and active component B through preliminary experiment.
The growth regulation effect of the mixed liquid of table 18 Brevibacillus laterosporus and active component B and different proportion thereof to tomato
As shown in Table 18, Brevibacillus laterosporus mixes with active component B after use, metabolic system that can activated plant and plant growth quickening vigor is strengthened, promote plant cell to increase, improving activity of root system is strengthened, reach volume increase object, Brevibacillus laterosporus and active component B, in the time of 1 ︰ 800 ~ 40 ︰ 1, all have stronger regulating action.
Find through test: after Brevibacillus laterosporus and active component B are composite, the control of the powdery mildew in various crop, downy mildew, virus disease, head blight, fusarium wilt, gray mold, epidemic disease, leaf mold, shot hole, scab, peronospora tabacina, anthracnose, spot defoliation, rust, rice blast, false smut, banded sclerotial blight, brown spot or leaf blight is had to obvious synergistic effect, synergy ratio SR is all greater than 1.5.
Effect experiment part: test medicine is researched and developed, provided by Shanxi Thompson Biological Technology Co., Ltd..
Table 19 test medicine
Application Example 14 Brevibacillus laterosporus and part active component B and composite powdery mildew of cucumber drug effect and the growth regulation Experiment on Function prevented and treated thereof
This test arrangement is at Xian District, Shanxi Province suburban area, and investigation powdery mildew of cucumber disease index before medicine, in the dispenser of disease initial stage of origination, investigates disease indexes and calculate preventive effect, the growth rate of dispenser " Invest, Then Investigate " crop for the second time for after dispenser 5 days, 15 days, 30 days.Result of the test is as follows:
Composite powdery mildew of cucumber drug effect and the growth regulation Experiment on Function prevented and treated of table 20 Brevibacillus laterosporus and part active component B
As can be seen from Table 20, after Brevibacillus laterosporus and part active component B are composite, can effectively prevent and treat powdery mildew of cucumber, test and find that Brevibacillus laterosporus and part active component B also can effectively prevent and treat cucumber downy mildew, epidemic disease after composite through applicant, control efficiency is all higher than 96%, be better than the preventive effect of single dose, the preventive effect phase is long, and metabolic system that can activated plant and plant growth quickening vigor is strengthened, and strengthens the growth of root system.In test scope of medication, target crop is had no adverse effects simultaneously.
Application Example 15 Brevibacillus laterosporus and part active component B and composite wheat rust drug effect and the growth regulation Experiment on Function prevented and treated thereof
This test arrangement is in Weinan City Pucheng County, Shaanxi Province, and investigation wheat rust disease index before medicine, in the dispenser of disease initial stage of origination, investigates disease indexes and calculate preventive effect, the growth rate of dispenser " Invest, Then Investigate " crop for the second time for after dispenser 3 days, 10 days, 20 days.Result of the test is as follows:
Table 21 Brevibacillus laterosporus and part active component B and composite wheat rust drug effect and the growth regulation Experiment on Function prevented and treated thereof
As can be seen from Table 21, after Brevibacillus laterosporus and part active component B are composite, can effectively prevent and treat wheat rust, test and find that Brevibacillus laterosporus and part active component B also can effectively prevent and treat wheat scab, brown spot, leaf blight after composite through applicant, control efficiency is all higher than 96%, be better than the preventive effect of single dose, the preventive effect phase is long, and metabolic system that can activated plant and plant growth quickening vigor is strengthened, and strengthens the growth of root system.In test scope of medication, target crop is had no adverse effects simultaneously.
Application Example 16 Brevibacillus laterosporus and part active component B and composite control pepper virus disease drug effect and growth regulation Experiment on Function
This test arrangement is at Xian District, Shanxi Province suburban area, and investigation pepper virus disease disease index before medicine, in the dispenser of disease initial stage of origination, investigates disease indexes and calculate preventive effect, the growth rate of dispenser " Invest, Then Investigate " crop for the second time for after dispenser 5 days, 15 days, 30 days.Result of the test is as follows:
Table 22 Brevibacillus laterosporus and part active component B and composite control pepper virus disease drug effect and growth regulation Experiment on Function
As can be seen from Table 22, Brevibacillus laterosporus and part active component B and composite control pepper virus disease thereof, through applicant test find Brevibacillus laterosporus and part active component B composite after also effectively mixed with rice bran, epidemic disease, control efficiency is all higher than 95%, be better than the preventive effect of single dose, the preventive effect phase is long, and metabolic system that can activated plant and plant growth quickening vigor is strengthened, and strengthens the growth of root system.In test scope of medication, target crop is had no adverse effects simultaneously.
Application Example 17 Brevibacillus laterosporus and part active component B and composite control watermelon blight drug effect and growth regulation Experiment on Function
This test arrangement is in Weinan City Dali County, Shaanxi Province, and investigation watermelon blight disease index before medicine, in the dispenser of disease initial stage of origination, investigates disease indexes and calculate preventive effect, the growth rate of dispenser " Invest, Then Investigate " crop for the second time for after dispenser 3 days, 10 days, 20 days.Result of the test is as follows:
Table 23 Brevibacillus laterosporus and part active component B composite control watermelon blight drug effect and growth regulation Experiment on Function
As can be seen from Table 23, after Brevibacillus laterosporus and part active component B are composite, can effectively prevent and treat watermelon blight, test and find that Brevibacillus laterosporus and part active component B also can effectively prevent and treat watermelon powdery mildew, fusarium wilt, epidemic disease after composite through applicant, control efficiency is all higher than 95%, be better than the preventive effect of single dose, the preventive effect phase is long, and metabolic system that can activated plant and plant growth quickening vigor is strengthened, and strengthens the growth of root system.In test scope of medication, target crop is had no adverse effects simultaneously.
Application Example 18 Brevibacillus laterosporus and part active component B and composite control citrus anthracnose drug effect and growth regulation Experiment on Function
This test arrangement is in Hanzhong City suburb, Shaanxi Province, and investigation citrus anthracnose disease index before medicine, in the dispenser of disease initial stage of origination, investigates disease indexes and calculate preventive effect, the growth rate of dispenser " Invest, Then Investigate " crop for the second time for after dispenser 10 days, 20 days, 30 days.Result of the test is as follows:
Table 24 Brevibacillus laterosporus and part active component B composite control citrus anthracnose drug effect and growth regulation Experiment on Function
As can be seen from Table 24, after Brevibacillus laterosporus and part active component B are composite, can effectively prevent and treat citrus anthracnose, test and find that Brevibacillus laterosporus and part active component B also can effectively prevent and treat citrus scab after composite through applicant, control efficiency is all higher than 96%, be better than the preventive effect of single dose, the preventive effect phase is long, and metabolic system that can activated plant and plant growth quickening vigor is strengthened, and strengthens the growth of root system.In test scope of medication, target crop is had no adverse effects simultaneously.Application Example 19 Brevibacillus laterosporus and part active component B and composite downy mildew of garpe drug effect and the growth regulation Experiment on Function prevented and treated thereof
This test arrangement is in Weinan City Dali County, Shaanxi Province, and investigation downy mildew of garpe disease index before medicine, in the dispenser of disease initial stage of origination, investigates disease indexes and calculate preventive effect, the growth rate of dispenser " Invest, Then Investigate " crop for the second time for after dispenser 7 days, 14 days, 25 days.Result of the test is as follows:
Composite downy mildew of garpe drug effect and the growth regulation Experiment on Function prevented and treated of table 25 Brevibacillus laterosporus and part active component B
As can be seen from Table 25, after Brevibacillus laterosporus and part active component B are composite, can effectively prevent and treat downy mildew of garpe, test and find that Brevibacillus laterosporus and part active component B also can effectively prevent and treat grape powdery mildew after composite through applicant, control efficiency is all higher than 96%, be better than the preventive effect of single dose, the preventive effect phase is long, and metabolic system that can activated plant and plant growth quickening vigor is strengthened, and strengthens the growth of root system.In test scope of medication, target crop is had no adverse effects simultaneously.Application Example 20 Brevibacillus laterosporus and part active component B and composite control alternaria leaf spot of apple drug effect and growth regulation Experiment on Function
This test arrangement is at Shaanxi Province's Weinan City Baishui County, and investigation alternaria leaf spot of apple disease index before medicine, in the dispenser of disease initial stage of origination, investigates disease indexes and calculate preventive effect, the growth rate of dispenser " Invest, Then Investigate " crop for the second time for after dispenser 7 days, 14 days, 25 days.Result of the test is as follows:
Table 26 Brevibacillus laterosporus and part active component B composite control alternaria leaf spot of apple drug effect and growth regulation Experiment on Function
As can be seen from Table 26, after Brevibacillus laterosporus and part active component B are composite, can effectively prevent and treat alternaria leaf spot of apple, test and find that Brevibacillus laterosporus and part active component B also can effectively prevent and treat apple marssonina leaf spot, scab after composite through applicant, control efficiency is all higher than 95%, be better than the preventive effect of single dose, the preventive effect phase is long, and metabolic system that can activated plant and plant growth quickening vigor is strengthened, and strengthens the growth of root system.In test scope of medication, target crop is had no adverse effects simultaneously.
Application Example 21 Brevibacillus laterosporus and part active component B and composite control peronophythora litchi drug effect and growth regulation Experiment on Function
This test arrangement is in suburb, Zengcheng City of Guangdong Province, and investigation peronophythora litchi disease index before medicine, in the dispenser of disease initial stage of origination, investigates disease indexes and calculate preventive effect, the growth rate of dispenser " Invest, Then Investigate " crop for the second time for after dispenser 7 days, 14 days, 25 days.Result of the test is as follows:
Table 27 Brevibacillus laterosporus and part active component B and composite control peronophythora litchi drug effect and growth regulation Experiment on Function
As can be seen from Table 27, after Brevibacillus laterosporus and part active component B are composite, can effectively prevent and treat peronophythora litchi, test and find that Brevibacillus laterosporus and part active component B also can effectively prevent and treat lichee anthracnose after composite through applicant, control efficiency is all higher than 95%, be better than the preventive effect of single dose, the preventive effect phase is long, and metabolic system that can activated plant and plant growth quickening vigor is strengthened, and strengthens the growth of root system.In test scope of medication, target crop is had no adverse effects simultaneously.
After draw through different local throughout the country tests, Brevibacillus laterosporus and Fluoxastrobin, kresoxim-methyl, pyraclostrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, orysastrobin, SSF 126, azoles bacterium ester, ZJ0712, ZEN 90160 is composite rear to the powdery mildew in various crop, downy mildew, virus disease, head blight, fusarium wilt, gray mold, epidemic disease, leaf mold, shot hole, scab, peronospora tabacina, anthracnose, spot defoliation, rust, rice blast, false smut, banded sclerotial blight, the preventive effect of the Common Diseases such as brown spot or leaf blight is all more than 95%, be better than single dose preventive effect, synergistic effect is obvious.
Claims (6)
1. the efficient bactericidal composite containing Brevibacillus laterosporus, effective active composition is active components A and active component B, it is characterized in that: active components A and active component B percentage by weight are 0.1% ~ 20%:0.5% ~ 80%, described active components A is selected from Brevibacillus laterosporus, and active component B is selected from the one in Fluoxastrobin, kresoxim-methyl, pyraclostrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, orysastrobin, SSF 126, azoles bacterium ester, ZJ0712 or ZEN 90160.
2. the efficient bactericidal composite containing Brevibacillus laterosporus according to claim 1, is characterized in that: the percentage by weight of active components A and active component B is 0.5% ~ 15%:1% ~ 60%.
3. the efficient bactericidal composite containing Brevibacillus laterosporus according to claim 2, is characterized in that:
The percentage by weight of Brevibacillus laterosporus and Fluoxastrobin or SSF 126 or ZEN 90160 is 1% ~ 10%:5% ~ 60%;
The percentage by weight of Brevibacillus laterosporus and pyraclostrobin or kresoxim-methyl or oxime bacterium ester or dimoxystrobin or orysastrobin or fluoxastrobin is 1% ~ 10%:5% ~ 50%;
The percentage by weight of Brevibacillus laterosporus and azoles bacterium ester or ZJ0712 is 1% ~ 10%:2% ~ 45%.
4. according to the efficient bactericidal composite containing Brevibacillus laterosporus described in any one in claims 1 to 3, it is characterized in that: composition is made wetting powder, water dispersible granules, suspending agent, aqueous emulsion, microemulsion or microcapsule suspending agent.
5. the purposes of the efficient bactericidal composite containing Brevibacillus laterosporus according to claim 4 for preventing and treating crop pest and Promoting plant growth.
6. purposes according to claim 5, is characterized in that: described disease comprises powdery mildew, downy mildew, virus disease, head blight, fusarium wilt, gray mold, epidemic disease, leaf mold, shot hole, scab, peronospora tabacina, anthracnose, spot defoliation, rust, rice blast, false smut, banded sclerotial blight, brown spot or leaf blight.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577766A (en) * | 2016-12-14 | 2017-04-26 | 贵州省烟草科学研究院 | Biocontrol bacterium-pyraclostrobin composition as well as preparation method and use thereof |
| CN106689212A (en) * | 2016-12-14 | 2017-05-24 | 贵州省烟草科学研究院 | Bactericide composition containing azoxystrobin, and preparation method and application thereof |
| CN108998394A (en) * | 2018-08-16 | 2018-12-14 | 浙江农林大学 | A kind of bacillus and its application |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577766A (en) * | 2016-12-14 | 2017-04-26 | 贵州省烟草科学研究院 | Biocontrol bacterium-pyraclostrobin composition as well as preparation method and use thereof |
| CN106689212A (en) * | 2016-12-14 | 2017-05-24 | 贵州省烟草科学研究院 | Bactericide composition containing azoxystrobin, and preparation method and application thereof |
| CN106577766B (en) * | 2016-12-14 | 2019-01-11 | 贵州省烟草科学研究院 | A kind of composition, the preparation method and the usage of biocontrol bacteria and pyraclostrobin |
| CN106689212B (en) * | 2016-12-14 | 2019-08-30 | 贵州省烟草科学研究院 | A kind of microbicide compositions containing Fluoxastrobin, preparation method and application |
| CN108998394A (en) * | 2018-08-16 | 2018-12-14 | 浙江农林大学 | A kind of bacillus and its application |
| CN108998394B (en) * | 2018-08-16 | 2020-11-20 | 浙江农林大学 | Bacillus and application thereof |
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Application publication date: 20140716 |