CN101278680B - Insect baculovirus pesticides in dosage form of water dispersible granule and preparation - Google Patents
Insect baculovirus pesticides in dosage form of water dispersible granule and preparation Download PDFInfo
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- CN101278680B CN101278680B CN2007100652359A CN200710065235A CN101278680B CN 101278680 B CN101278680 B CN 101278680B CN 2007100652359 A CN2007100652359 A CN 2007100652359A CN 200710065235 A CN200710065235 A CN 200710065235A CN 101278680 B CN101278680 B CN 101278680B
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- 239000002552 dosage form Substances 0.000 title claims description 16
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- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 6
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Abstract
The invention relates to a water dispersion granula preparation formulation of an insect baculovirus pesticide and a preparation thereof, which is a solid water dispersion granula preparation formulation and comprises 10-500 billion PIB/g of the insect baculovirus raw powder and accessory agents, the accessory agents comprise vector, dispersant, wetting agent, disintegrant, light protective agent and bond. The proportion of weight portion of every component is as follows: 1 to 50 portions of the insect baculovirus raw powder, 1 to 70 portions of the vector, 1 to 20 portions of the dispersant, 0 to 20 portions of the wetting agent, 1 to 20 portions of the disintegrant, 0.5 to 20 portions of the light protective agent and 1 to 20 portions of the bond. The preparation method is that the insect baculovirus raw powder and the accessory agents are mixed by certain proportion to be crushed by airflow for granulating and drying to obtain the water dispersion granula preparation formulation of the insect baculovirus pesticide which can be used separately and also can be used by combing with chemical pesticide. The water dispersion granula preparation formulation of the insect baculovirus pesticide has the advantages of little pollution caused by producing and processing process, long shelf life, stable product quality, good dispersibility, easy storage, convenient transportation, extremely low dosage and high virus content, which has significant meaning for preventing and curing insects in agriculture and forestry.
Description
Technical field
The present invention relates to a kind of insect baculovirus pesticides and preparation method thereof, relate in particular to the insect baculovirus pesticides in dosage form of water dispersible granule and the preparation method of a kind of ultralow consumption, high-load.
Background technology
The main host of Rhabdoviridae (Baculoviridae) virus is an insect, and it is sick and dead to make host insect suffer from baculoviral, can make the insecticide control agriculture and forestry injurious insect.Insect baculovirus pesticides (abbreviation viral insecticide) is the biopesticide of a class pure natural, green, environmental protection.Current, the unit are consumption of chemical insecticide is more and more higher, also more and more serious to environment, non-target animal, bio-diversity and the human harm that causes itself, a large amount of severe toxicity, high residue insecticide are limited the production, cut-off, limit the use of and forbid, the situation that produce, the development and application viral insecticide is fast development, market demand rises year by year.
The production of viral insecticide is made up of two parts: the production of insect disease toxogen powder and the processing of virus insect disinfestation agent formulation.The basic principle of former powder production is exactly by allowing the oral corresponding virus of insect, make insect suffer from the insect disease viral disease, virus is amplification in a large number in insect bodies, extracts virus then and make former powder from the insect corpse of ill death, be can desinsection in the viral insecticide active component.Preparation processing is exactly to add material such as proper assistant in former powder, makes it to become to be directly used in the process that the virus insect disinfestation activity was sprayed and can bring into play to greatest extent in the field.
Because insect viruses are biological products that a class has life, in that former powder is processed into the process of preparation from virus, protect its insecticidal activity to be without prejudice and are even more important.For example in alkaline environment, the polyhedrin of virus can very fast dissolving, and the activity of virus is destroyed; In the medium-term and long-term preservation of the aqueous solution, very easily produce assorted bacterium in the product, have influence on the quality and the use of product; Too high or the ultraviolet radiation of environmental temperature etc. can cause the insecticidal activity of virus sharply to descend.Thereby develop be suitable for storing, transportation, and the viral insecticide formulation product of effect stability, most important to the large-scale promotion application of this class biopesticide.
Compare with chemical insecticide, the kind of insect baculovirus pesticides exploitation is few, and in evening time that research and development are carried out, research basis and level are relatively low.The research and development of PI toxin preparation all are that technology and the method for using for reference chemical insecticide are carried out, yet the physicochemical property of insect baculovirus and insecticidal mechanism have distinct characteristics, some technology of imitation chemical insecticide processing, for the performance of insect viruses insecticidal action, be unnecessary or even harmful.
The formulation of having registered at present the insect virus pesticide product mainly contains two kinds of wetting powder and suspending agents, virus polyhedron content is generally at 5-20 hundred million PIB/mL (gram), viral level is low in the product, the inserts too high levels, field dosage big (general field mu consumption 40-100mL (gram) or more than), make troubles in packing, store, transportation, sell, and product a series of problems such as denaturation of virus inactivation in the producing and selling process.The for example processing of wetting powder in the viral insecticide, with reference to the technology of conventional chemical insecticide wetting powder, add the inertia filler of a large amount of (60%-90%), as diatomite, kaolin etc., caused the process dust pollution like this, viral level reduces in the preparation.With heliothis armigera nuclear polyhedrosis virus (Helicoverpa armigera nuclear polyhedrosis virus, be called for short HaNPV) be example, the content of former medicine is at 100-200 hundred million PIB/mL (gram), when process preparation, a large amount of fillers and a certain amount of auxiliary agent have been added, thereby the polyhedron content that makes the heliothis armigera nuclear polyhedrosis virus preparation is between 10-20 hundred million PIB/mL (gram), to being diluted in about 10 times of former concentration.For example existing wetting powder product has, the Henan Province rich biochemical pesticide of universal love favour Co., Ltd registration product: 1,000,000,000 PIB/ gram heliothis armigera nuclear polyhedrosis virus wetting powder, registration card number: LS94579; Chibi, the Hubei Province sincere biotechnology of will Co., Ltd registration product: 1,000,000,000 PIB/ gram heliothis armigera nuclear polyhedrosis virus wetting powder, registration card number: LS20011685 etc.The suspending agent product has, Shijiazhuang City, Hebei Province city ring industry biopesticide factory registration product: 2,000,000,000 PIB/ milliliter heliothis armigera nuclear polyhedrosis virus suspending agents, registration card number: LS96795; The registration product of Zhuhai City, Guangdong Province China biologic product Co., Ltd: 2,000,000,000 PIB/ milliliter heliothis armigera nuclear polyhedrosis virus suspending agents, registration card number: LS20031797 etc.
Wetting powder and suspending agent respectively have pluses and minuses in transportation and application: be not easy to the preservation transportation as suspending agent, though and wetting powder is better than suspending agent aspect active keeping viral organism, but because under the situation of preparation thin up when using in the field, the technical merit that is added man-hour limits, the polyhedrosis dispersiveness of virus is good not as suspending agent, thereby causes the field effect to be lower than suspending agent.
Summary of the invention
The invention provides a kind of shortcoming that has overcome suspending agent and wetting powder, good dispersion, easily preservation, convenient transportation, ultralow consumption, high-load be the insect baculovirus pesticides in dosage form of water dispersible granule and the preparation method of main active with the insect baculovirus.
Technical scheme of the present invention is as follows:
Insect baculovirus pesticides in dosage form of water dispersible granule provided by the invention is characterised in that, described insect baculovirus pesticides in dosage form of water dispersible granule is the solid dosage form of water dispersible granule, comprises former powder of insect baculovirus and auxiliary agent; The insect baculovirus content of the former powder of described insect baculovirus is 100-5000 hundred million PIB/ grams; Described auxiliary agent comprises carrier, dispersant, wetting agent, disintegrant, bright protective agent and binding agent;
The weight portion proportioning of described each component is as follows:
The former powder 1-50 of insect baculovirus weight portion;
Carrier 1-70 weight portion;
Dispersant 1-20 weight portion;
Wetting agent 0-20 weight portion;
Disintegrant 1-20 weight portion;
Bright protective agent 0.5-20 weight portion;
Binding agent 1-20 weight portion.
The former powder of described insect baculovirus is that nuclear polyhedrosis virus (nuclear polyhedrosis virus) former powder is or/and the former powder of PuGV (granulosis virus).
Described carrier is white carbon, bentonite, kaolin, diatomite or calcium carbonate etc.;
Described dispersant is a kind of surface reactive material, can be anion surfactant, cationic surfactant, amphoteric surfactant and non-ionic surface active agent; More preferably dodecyl sodium laurate, neopelex, lauryl sodium sulfate, lignin sodium, lignin calcium or Tea Saponin etc.;
Described wetting agent is a kind of surface reactive material, can be anion surfactant, cationic surfactant, amphoteric surfactant and non-ionic surface active agent; More preferably dodecyl sodium laurate, neopelex, lauryl sodium sulfate, lignin sodium, lignin calcium or Tea Saponin etc.;
Described disintegrant is Ac-Di-Sol, crosslinked carboxymethyl fecula sodium, sulfuric acid amine, sodium bicarbonate, starch or urea etc.;
Described bright protective agent is fluorescent whitening agent, india ink, nano titanium oxide, nano zine oxide or activated carbon powder etc.;
Described binding agent is soluble starch, gelatin, polyvinyl alcohol, carboxymethyl cellulose or sodium carboxymethylcellulose etc.
The preparation method of insect baculovirus pesticides in dosage form of water dispersible granule provided by the invention, its step is as follows:
1) the former powder of insect baculovirus, carrier, dispersant, wetting agent, disintegrant, bright protective agent and binding agent are mixed by following weight portion proportioning and carry out precomminution;
2) will pass through comminution by gas stream again through the raw material of precomminution, and make its particle diameter less than 10 μ m;
3) in mixer, the raw material behind comminution by gas stream is mixed once more;
4) add water and mediate, use the comminutor extruder grain; Perhaps use the thermopnore granulation;
5) afterwards it is carried out vacuum drying or freeze drying, baking temperature is not higher than 40 ℃, obtains moisture and is no more than 5% water dispersible granular thing;
6) resulting water dispersible granular thing is sieved, promptly make insect baculovirus pesticides in dosage form of water dispersible granule.
Insect baculovirus pesticides in dosage form of water dispersible granule of the present invention can use separately, also can unite use with chemical insecticide, is applied to prevent and treat agriculture and forestry injurious insect.
With respect to insect baculovirus suspending agent and wetting powder, insect baculovirus pesticides in dosage form of water dispersible granule of the present invention has many irreplaceable advantages: (1) process and field application process dust pollution are very little, to operator's safety; (2) moisture very little (being lower than 5%), the active retention time of viral organism is long, and shelf life is long, constant product quality; (3) environmental temperature of process is not higher than 40 ℃, has kept viral insecticidal activity to greatest extent; (4) viral level height (between 100-5000 hundred million PIB/ gram), good disinsection effect, the field consumption is few, is convenient to transportation; (5) it is few that good fluidity, container adhere to, add water after disintegrative good, easy to use; (6) because of the active constituent content height, dispersant in the auxiliary agent and bright protective agent can play one's part to the full, and make that viral polyhedron is scattered in the field, anti-uv-ray is strong; (7) adjuvant used all is the Water-borne modification material, adds field consumption considerably less (every mu of ground only needs the 0.2-10 gram), very friendly to environment, is the pesticide product of environmental protection.
Embodiment
Embodiment 1
The former powder 2Kg of heliothis armigera nuclear polyhedrosis virus of 5,000 hundred million PIB/ gram adds neopelex (also can be the dodecyl sodium laurate) 10Kg, lignin calcium 10Kg, urea 5Kg, fluorescent whitening agent (also can be nano zine oxide) 2Kg, kaolin 66Kg, carboxymethyl cellulose 5Kg.Cracker mixes, pulverizes, and becomes female powder.With airslide disintegrating mill female powder is further pulverized, made particle diameter, in mixer, fully mix then less than 10 μ m.After the thermopnore granulation, vacuum drying, baking temperature is controlled at 30 ℃.The check moisture is lower than at 5% o'clock, gets material and sieves, and yield rate is 95%.Promptly obtain 10,000,000,000 PIB/ gram heliothis armigera nuclear polyhedrosis virus water dispersible granules product 95Kg, need the bollworm virus of 1,000 hundred million PIB to prevent and treat cotton bollworm by every mu of cotton field, then its field consumption is 10 gram/mus.
During field control, water 40Kg spraying is converted with water dispersible granules 10 grams that 10,000,000,000 PIB/ restrain in every mu of cotton field, and the control efficiency to cotton bollworm after 7 days reaches more than 80%, and to natural enemies security.
Embodiment 2
The former powder 15Kg of prodenia litura nuclear polyhedrosis virus (Spodoptera litura nuclearpolyhedrosis virus) of 2,000 hundred million PIB/ gram, add lauryl sodium sulfate 15Kg, Tea Saponin (also can be lignin calcium) 12Kg, sulfuric acid amine (also can be sodium bicarbonate) 15Kg, nano titanium oxide 5Kg, bentonite 32Kg, soluble starch 6Kg.Cracker mixes, pulverizes, and becomes female powder.With airslide disintegrating mill female powder is further pulverized, made particle diameter, in mixer, fully mix then less than 10 μ m.Behind the extruder grain, vacuum drying, baking temperature is controlled at 30 ℃.The check moisture is lower than at 5% o'clock, gets material and sieves, and yield rate is 95%.Promptly obtain 30,000,000,000 PIB/ gram prodenia litura nuclear polyhedrosis virus water dispersible granules product 95Kg, by the prodenia litura prevention and cure of viruses prodenia litura of every mu of vegetables field needs 60,000,000,000 PIB, then its field consumption is 2 gram/mus.
During field control, water 30Kg spraying is converted with water dispersible granules 2 grams that 30,000,000,000 PIB/ restrain in every mu of vegetables field, and the control efficiency to prodenia litura after 7 days reaches more than 80%, and to natural enemies security.
Embodiment 3
The former powder 10Kg of plutella xylostella granulosis virus (Plutella xylostella granulosis virus) of 6,000 hundred million PIB/ gram adds Tea Saponin 15Kg, starch 5Kg, fluorescent whitening agent 15Kg, bentonite 49Kg, polyvinyl alcohol 6Kg.Cracker mixes, pulverizes, and becomes female powder.With airslide disintegrating mill female powder is further pulverized, made particle diameter, in mixer, fully mix then less than 10 μ m.Behind the extruder grain, vacuum freezedrying.The dry end got material and sieves, and yield rate is 90%.Promptly obtain 60,000,000,000 PIB/ gram plutella xylostella granulosis virus water dispersible granules product 90Kg, by the diamond-back moth prevention and cure of viruses diamond-back moth of every mu of vegetables field needs 6,000 hundred million PIB, then its field consumption is 10 gram/mus.
During field control, water 30Kg spraying is converted with water dispersible granules 10 grams that 60,000,000,000 PIB/ restrain in every mu of vegetables field, and the control efficiency to diamond-back moth after 5 days reaches more than 85%, and to natural enemies security.
Embodiment 4
The former powder 1Kg of cabbage caterpillar PuGV (Pieris rapae granulosis virus) of 10,000 hundred million PIB/ gram adds lauryl sodium sulfate 1Kg, lignin calcium 20Kg, crosslinked carboxymethyl fecula sodium 20Kg, activated carbon powder 20Kg, calcium carbonate 37Kg, gelatin 1Kg.Cracker mixes, pulverizes, and becomes female powder.With airslide disintegrating mill female powder is further pulverized, made particle diameter, in mixer, fully mix then less than 10 μ m.Behind the extruder grain, vacuum freezedrying.The dry end got material and sieves, and yield rate is 98%.Promptly obtain 10,000,000,000 PIB/ gram cabbage caterpillar PuGV water dispersible granules product 98Kg, by the cabbage caterpillar prevention and cure of viruses cabbage caterpillar of every mu of vegetables field needs 1,000 hundred million PIB, then its field consumption is 10 gram/mus.
During field control, water 30Kg spraying is converted with water dispersible granules 10 grams that 10,000,000,000 PIB/ restrain in every mu of vegetables field, and the control efficiency to cabbage caterpillar after 6 days reaches more than 87%, and to natural enemies security.
Embodiment 5
The former powder 25Kg of laphygma exigua nuclear polyhedrosis virus (Spodoptera exigua nuclearpolyhedrosis virus) of 3,000 hundred million PIB/ gram, add Tea Saponin 10Kg, neopelex 10Kg, cross-linked carboxymethyl cellulose is received 12Kg, india ink 0.5Kg, calcium carbonate 32.5Kg, polyvinyl alcohol 10Kg.Cracker mixes, pulverizes, and becomes female powder.With airslide disintegrating mill female powder is further pulverized, made particle diameter, in mixer, fully mix then less than 10 μ m.Behind the extruder grain, vacuum freezedrying.The dry end got material and sieves, and yield rate is 95%.Promptly obtain 75,000,000,000 PIB/ gram laphygma exigua nuclear polyhedrosis virus water dispersible granules product 95Kg, by the beet armyworm prevention and cure of viruses beet armyworm of every mu of vegetables field needs 75,000,000,000 PIB, then its field consumption is 1 gram/mu.
During field control, water 30Kg spraying is converted with water dispersible granules 1 gram that 75,000,000,000 PIB/ restrain in every mu of vegetables field, and the control efficiency to beet armyworm after 5 days reaches more than 90%, and to natural enemies security.
Embodiment 6
The former powder 50Kg of tea-geometrid-type polyhedrosis viruses (Ectropis obliqua nuclearpolyhedrosis virus) of 1,000 hundred million PIB/ gram adds Tea Saponin 15Kg, urea 5Kg, fluorescent whitening agent 20Kg, diatomite 9Kg, soluble starch 1Kg.Cracker mixes, pulverizes, and becomes female powder.With airslide disintegrating mill female powder is further pulverized, made particle diameter, in mixer, fully mix then less than 10 μ m.Behind the extruder grain, vacuum freezedrying.The dry end got material and sieves, and yield rate is 99%.Promptly obtain 50,000,000,000 PIB/ gram tea-geometrid-type polyhedrosis viruses water dispersible granules product 99Kg, by the tea geometrid prevention and cure of viruses tea geometrid of every mu of tea place needs 1,000 hundred million PIB, then its field consumption is 2 gram/mus.
During control, water 40Kg spraying is converted with water dispersible granules 2 grams that 1,000 hundred million PIB/ restrain in every mu of tea place, and the control efficiency to tea geometrid after 9 days reaches more than 90%, and to natural enemies security.
Embodiment 7
The former powder 50Kg of agrotis segetum granulosis virus (Agrotis segetum granulosis virus) of 10,000 hundred million PIB/ gram adds lignin calcium 10Kg, dodecyl sodium laurate 2Kg, starch 3Kg, nano titanium oxide 10Kg, white carbon 5Kg, carboxymethyl cellulose 20Kg.Cracker mixes, pulverizes, and becomes female powder.With airslide disintegrating mill female powder is further pulverized, made particle diameter, in mixer, fully mix then less than 10 μ m.Behind the extruder grain, vacuum freezedrying.The dry end got material and sieves, and yield rate is 94%.Promptly obtain 5,000 hundred million PIB/ gram agrotis segetum granulosis virus water dispersible granules product 94Kg, by the white line dart moth of turnip prevention and cure of viruses white line dart moth of turnip of every mu of farmland needs 7,500 hundred million PIB, then its field consumption is 1.5 gram/mus.
During control, water 30Kg spraying is converted with water dispersible granules 1.5 grams that 10,000 hundred million PIB/ restrain in every mu of farmland, and the control efficiency to white line dart moth of turnip after 7 days reaches more than 90%, and to natural enemies security.
Embodiment 8
The former powder 10Kg of laphygma exigua nuclear polyhedrosis virus of 3,000 hundred million PIB/ gram, the former powder 40Kg of plutella xylostella granulosis virus of 6,000 hundred million PIB/ gram adds Tea Saponin 2Kg, lignin calcium 2Kg, cross-linked carboxymethyl cellulose is received 10Kg, nano zine oxide 1Kg, diatomite 25Kg, polyvinyl alcohol 10Kg.Cracker mixes, pulverizes, and becomes female powder.With airslide disintegrating mill female powder is further pulverized, made particle diameter, in mixer, fully mix then less than 10 μ m.Behind the extruder grain, vacuum freezedrying.The dry end got material and sieves, and yield rate is 96%.Promptly obtain the water dispersible granules product 96Kg that 30,000,000,000 PIB/ gram laphygma exigua nuclear polyhedrosis virus and 2,400 hundred million PIB/ gram plutella xylostella granulosis virus mix, the beet armyworm prevention and cure of viruses beet armyworm that needs 75,000,000,000 PIB by every mu of vegetables field, the plutella xylostella granulosis virus control diamond-back moth meter of 6,000 hundred million PIB, in the mixed vegetables field that takes place of two kinds of insects, its field consumption is 5 gram/mus.
Beet armyworm and the mixed vegetables Tanaka of taking place of diamond-back moth, the water dispersible granules product (30,000,000,000 PIB/ gram laphygma exigua nuclear polyhedrosis virus and 2,400 hundred million PIB/ gram plutella xylostella granulosis virus) 5 that mixes with two kinds of viruses restrains, convert water 30Kg spraying, control efficiency to beet armyworm and diamond-back moth after 5 days reaches more than 90% respectively, and to natural enemies security.
Claims (4)
1. an insect baculovirus pesticides in dosage form of water dispersible granule is characterized in that, described insect baculovirus pesticides in dosage form of water dispersible granule is the solid dosage form of water dispersible granule, comprises former powder of insect baculovirus and auxiliary agent; The insect baculovirus content of the former powder of described insect baculovirus is 100-5000 hundred million PIB/ grams; Described auxiliary agent comprises carrier, dispersant, wetting agent, disintegrant, bright protective agent and binding agent;
The weight portion proportioning of described each component is as follows:
The former powder 1-50 of insect baculovirus weight portion, its particle diameter is less than 10 μ m;
Carrier 1-70 weight portion, it is bentonite or calcium carbonate;
Dispersant 1-20 weight portion, it is dodecyl sodium laurate, neopelex, lauryl sodium sulfate or Tea Saponin;
Wetting agent 0-20 weight portion, it is dodecyl sodium laurate, neopelex, lauryl sodium sulfate, lignin sodium, lignin calcium or Tea Saponin;
Disintegrant 1-20 weight portion, it is Ac-Di-Sol or crosslinked carboxymethyl fecula sodium;
Bright protective agent 0.5-20 weight portion;
Binding agent 1-20 weight portion, it is soluble starch, gelatin or sodium carboxymethylcellulose;
The former powder of described insect baculovirus is that nuclear polyhedrosis virus is or/and PuGV.
2. by the described insect baculovirus pesticides in dosage form of water dispersible granule of claim 1, it is characterized in that described bright protective agent is fluorescent whitening agent, india ink, nano titanium oxide, nano zine oxide or activated carbon powder.
3. the preparation method of the described insect baculovirus pesticides in dosage form of water dispersible granule of claim 1, its step is as follows:
1) the former powder of insect baculovirus, carrier, dispersant, wetting agent, disintegrant, bright protective agent and binding agent are mixed by following weight portion proportioning and carry out precomminution;
The weight portion proportioning of described each component is as follows:
The former powder 1-50 of insect baculovirus weight portion;
Carrier 1-70 weight portion, it is bentonite or calcium carbonate;
Dispersant 1-20 weight portion, it is dodecyl sodium laurate, neopelex, lauryl sodium sulfate or Tea Saponin;
Wetting agent 0-20 weight portion, it is dodecyl sodium laurate, neopelex, lauryl sodium sulfate, lignin sodium, lignin calcium or Tea Saponin;
Disintegrant 1-20 weight portion, it is Ac-Di-Sol or crosslinked carboxymethyl fecula sodium;
Bright protective agent 0.5-20 weight portion;
Binding agent 1-20 weight portion, it is soluble starch, gelatin or sodium carboxymethylcellulose;
The former powder of described insect baculovirus is that nuclear polyhedrosis virus is or/and PuGV;
2) will pass through comminution by gas stream again through the raw material of precomminution, and make its particle diameter less than 10 μ m;
3) in mixer, the raw material behind comminution by gas stream is mixed once more;
4) add water and mediate, use the comminutor extruder grain; Perhaps use the thermopnore granulation;
5) afterwards it is carried out vacuum drying or freeze drying, baking temperature is not higher than 40 ℃, obtains moisture and is no more than 5% water dispersible granular thing;
6) resulting water dispersible granular thing is sieved, promptly make insect baculovirus pesticides in dosage form of water dispersible granule.
4. by the preparation method of the described insect baculovirus pesticides in dosage form of water dispersible granule of claim 1, it is characterized in that described bright protective agent is fluorescent whitening agent, india ink, nano titanium oxide, nano zine oxide or activated carbon powder.
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| CN102415374B (en) * | 2011-10-20 | 2013-10-09 | 迈克斯(如东)化工有限公司 | Trinexapacethyl water dispersible granule preparation and preparation method thereof |
| CN103563991A (en) * | 2012-07-31 | 2014-02-12 | 江苏丘陵地区镇江农业科学研究所 | Preparation method of brevibacillus brevis water dispersible granules |
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