CN106119154A - A kind of bacterial strain preventing and treating Peanut continuous cropping silborne fungal diseases and application - Google Patents
A kind of bacterial strain preventing and treating Peanut continuous cropping silborne fungal diseases and application Download PDFInfo
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- CN106119154A CN106119154A CN201610475283.4A CN201610475283A CN106119154A CN 106119154 A CN106119154 A CN 106119154A CN 201610475283 A CN201610475283 A CN 201610475283A CN 106119154 A CN106119154 A CN 106119154A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/38—Pseudomonas
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The present invention relates to a kind of bacterial isolates preventing and treating Peanut continuous cropping soil fungi sexually transmitted disease (STD) evil and microbial inoculum thereof, described bacterial strain is a kind of pseudomonas (Pseudomonas protegens), and deposit number is CGMCC No.11539.Bacterial strain 16s rRNA base sequence is as shown in SEQ ID No:1.Bacterial strain product chitinase (the degraded pathogen hyphal cell wall) activity of the present invention is higher, and the multiple soil-borne fungus pathogen mycelium such as Fusarium spp., rhizoctonia, Verticillium are all had notable degradation capability.Microbial inoculum of the present invention seed dressing consumption normally only 20 30 kgs/acre, can effectively prevent and treat the soil-borne fungus sexually transmitted disease (STD) evil that nonirrigated farmland Peanut continuous cropping root rot, damping-off, southern blight etc. take place frequently, preventive effect reaches more than 55%, there is the advantages such as consumption is little, antimicrobial spectrum wide, obvious effect of increasing production, effectively alleviate the soil-borne disease impact on continuous cropping Radix Polygalae Crotalarioidis production loss.
Description
Technical field
The present invention relates to bacterial strain and the bacterial preparation process thereof etc. done harm to for Peanut continuous cropping soil-borne fungus sexually transmitted disease (STD), belong to agricultural
Intensive manufacture technical field, is exclusively used in and overcomes Peanut continuous cropping silborne fungal diseases problem occurred frequently.
Background technology
Semen arachidis hypogaeae is the important oil crop of China, and long-term sown area accounts for about the 35% of the total sown area of oil crop.
Steep hilly region in southern China is one of Semen arachidis hypogaeae main producing region of China, relatively compared with main producing region, North China, South Red Soil Region peanut yield
Relatively low (average product is only about the 60% of producing region, North China), this is both relevant with peanut varieties, weather conditions, soil fertility etc.,
Also seriously there is certain relation with South Red Soil Region Semen arachidis hypogaeae continuous cropping obstacle.Owing to red soil slopeland fertility is relatively low, seasonal drought is tight
Weight, Semen arachidis hypogaeae continuous cropping phenomenon is universal.Semen arachidis hypogaeae continuous cropping continuous cropping often leads to the evil aggravation of soil-borne fungus sexually transmitted disease (STD), increases with the continuous cropping time limit, flower
Maize ear rot of taking root sickness rate rises at double, and damping-off and southern blight persistently increase.Therefore, continuous cropping Radix Polygalae Crotalarioidis soil-borne fungus is effectively prevented and treated
Sexually transmitted disease (STD) evil becomes the current demand that the sustainable production of Semen arachidis hypogaeae is urgent.
The most conventional administering method has to be used chemical bactericide and uses the biological organic fertilizer containing biocontrol microorganisms.Chemistry bacterium
Soil surface characters radix can effectively be killed by agent or efficiently fumigant, temporarily alleviates disease and occurs, but also inhibits soil simultaneously
Probiotics, in the long run/term, chemosterilization will deteriorate soil ecology function further, is unfavorable for agricultural production.Biological and ecological methods to prevent plant disease, pests, and erosion
The screening of bacterium is key prepared by biological organic fertilizer, and the screening of current biocontrol agent is mainly from growth-promoting, metabolite biocidal property
Use after angle is carried out, and generally employing and fertilizer carry out mixed fermentation.But in view of biocontrol microorganisms is a kind of living body biological, no
It is difficult to directly at fertilizer continued survival etc. through strengthening induction, causes field control effect unstable.Additionally, it is the most existing
The general large usage quantity of Biological control fertilizer (> 200 kgs/acre), application cost higher.
Generally, continuous cropping ground soil-borne disease fungal pathogens abundance of a relatively high (> 30%), the cell wall of these pathogenic fungi
It is by there being chitin to constitute.
Summary of the invention
It is an object of the invention to solve the deficiency that prior art exists, it is provided that one is caused by pathogenic fungi for preventing and treating
The effective preparation method and applications of biocontrol agent of Semen arachidis hypogaeae soil-borne disease, thus effectively reduce Peanut continuous cropping rhizosphere pathogenic fungi
Radix, reaches to suppress the purpose of soil-borne pathogen infecting peanut root, and the inventive method specialization is strong, effect stability.
For achieving the above object, the present invention is by the following technical solutions:
A kind of pseudomonas (Pseudomonas protegens) WXX-1, deposit number is CGMCC No.11539.Institute
Stating bacterial strain is gram-negative bacterium, and the 16s rDNA base sequence of bacterial strain is as shown in SEQ ID No:1.
The invention also discloses the application in preventing and treating soil-borne fungus sexually transmitted disease (STD) evil of the described bacterial strain.Particularly at preventing and treating red soil
Application in Peanut continuous cropping soil-borne fungus sexually transmitted disease (STD) evil.
Present invention also offers bacterial strain application in preparation preventing and treating soil-borne fungus sexually transmitted disease (STD) evil microbial inoculum.
A kind of microbial inoculum preventing and treating soil-borne fungus sexually transmitted disease (STD) evil, this microbial inoculum is prepared by pseudomonas WXX-1.
Further, in this microbial inoculum, pseudomonas WXX-1 is through fungal mycelium induction strengthening, effectively survives in having of becoming thoroughly decomposed
In machine muck, the content of WXX-1 is 1 × 109More than cfu/g.The suitability of microbial inoculum field is strong, may be directly applied to prevent and treat southern red
Earth Peanut continuous cropping soil-borne fungus sexually transmitted disease (STD) evil.
The present invention provides the pseudomonas WXX1 microbial inoculum technology of preparing to be:
1) inactivation saprophytic fungus mycelia inductor obtains: configuration PD culture fluid (Rhizoma Solani tuber osi 20% (g/ml), glucose
1.5% (g/ml), distilled water 1000ml), and sterilization treatment；Then inoculate Mortierella (Mortierella sp.), be placed in constant temperature
Shaking table dark culturing (28 DEG C, 120rpm), after 7d, filter paper filtering obtains mycelia, distilled water wash 2 times, high temperature sterilize after sucking filtration
(121 DEG C, 20min) are standby.
2) configuration biocidal property enriched medium: Carnis Bovis seu Bubali cream 3% (g/ml), peptone 8% (g/ml), inactivate saprophytic fungus bacterium
Filament 3% (g/ml)；
3) strain stability strengthening: added by described bacterial strain in above-mentioned culture medium, ferment ventilation 10L/m, speed of agitator
200r/min, with salt acid for adjusting pH value 5.5-6.5, fermentation temperature is 26 DEG C, fermentation time 72h, and recording bacterium solution OD value is 1.0 left sides
Right；
4) prepared by described microbial inoculum: with the pig manure that becomes thoroughly decomposed as substrate, by above-mentioned WXX1 biocidal property forced fermentation liquid by 5%
Ratio is inoculated into above-mentioned substrate, places calorstat (28 DEG C) and cultivates 5d；Parasitic to fungal mycelia to described bacterial strain through second step
Strengthening, described microbial inoculum can grow the most surely in the organic substrate containing a large amount of saprophytic fungus, described bacterium amount reaches 1 ×
109cfu/g.Finally obtain the solids mixing microbial inoculum of WXX1 bacteriostasis property strengthening.
The application process of microbial inoculum of the present invention, is to be puddled with microbial inoculum by peanut seed in 1:2 ratio (weight ratio), microbial inoculum
Consumption 20-30 kg/acre；After field trench digging, together with the peanut seed after mixing thoroughly, it is sowed at the seed furrow held successfully in advance with microbial inoculum
In, then earthing.So on the one hand, described bacterial strain can be grown in Semen arachidis hypogaeae rhizosphere the most surely, on the other hand can be effectively attached at Roots of Peanut circle
Closely form protecting wall, reach to suppress the purpose of soil-borne pathogen infecting peanut root.The present invention can effectively prevent and treat continuous cropping Radix Polygalae Crotalarioidis
Root rot, damping-off, base rot disease, southern blight, preventive effect reaches more than 55%, has that field control effect is stable, antimicrobial spectrum is wide.
The invention have the advantage that bacterial strain WXX1 produces chitinase (degraded pathogen hyphal cell wall) activity higher, to sickle
The multiple soil-borne fungus pathogen mycelium such as cutter bacterium, rhizoctonia, Verticillium all have notable degradation capability, can through strengthening induction
With effectively parasitic existence on saprophytic quasi-microorganism in fertilizer；It is inoculated in the organic manure that becomes thoroughly decomposed and is prepared as solid fungicide；It is
For preventing and treating the biocontrol bacteria of Peanut continuous cropping soil-borne fungus sexually transmitted disease (STD) evil, microbial inoculum consumption is few, low cost, obvious effect of increasing production.Soil passes
Fungal disease is the Major Diseases occurred during intensive agriculture produces, and can cause crop withered death, and the underproduction is serious.If pressing
Microbial inoculum prepared by said method applies Peanut continuous cropping rhizosphere, so that it may stable existence within the peanut growth phase, thus is effectively formed and presses down
The protecting wall of pathogen infection processed, and effectively reduce Semen arachidis hypogaeae rhizosphere pathogen radix, finally reach with " competition " between microorganism
To the purpose effectively preventing and treating Peanut continuous cropping silborne fungal diseases.
Accompanying drawing explanation
Fig. 1 is pseudomonas WXX1 growth in culture medium (chitin containing 2%) and transparent circle.
Fig. 2 is the pseudomonas WXX1 experiment effect figure to Fusarium oxysporum.
Note: in figure, 1 is Fusarium oxysporum, 2 is pseudomonas WXX1
Fig. 3 is the pseudomonas WXX1 experiment effect figure to Fusarium solani.
Note: in figure, 1 is Fusarium solani, 2 is pseudomonas WXX1
Fig. 4 is the experiment effect figure of pseudomonas WXX1 Rhizoctonia solani.
Note: in figure, 1 is Rhizoctonia solani Kuhn, 2 is pseudomonas WXX1
Fig. 5 is the pseudomonas WXX1 experiment effect figure to Verticillium.
Note: in figure, 1 is Verticillium, 2 is pseudomonas WXX1
Fig. 6 is the Electronic Speculum figure that pathogen fungal mycelia is destroyed by pseudomonas WXX1.
Fig. 7 is pseudomonas WXX1 microbial inoculum Field information design sketch.
Bacterial strain WXX-1 in the present invention, the Classification And Nomenclature of described bacterial strain is: pseudomonas (Pseudomonas
Protegens), deposit number is CGMCC No.11539, and depositary institution is that China Committee for Culture Collection of Microorganisms is general
Logical microorganism center (CGMCC), depositary institution address is: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the Chinese Academy of Sciences is micro-
Biological study institute, preservation date is on October 26th, 2015.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearer, below in conjunction with drawings and Examples, right
The present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, and
It is not used in the restriction present invention.
Embodiment 1:
Gathering the test that the Semen arachidis hypogaeae rhizosphere soil on one piece of Peanut continuous cropping ground, red earth area carries out being correlated with, it concretely comprises the following steps:
(1) soil sample processes: prepare the enrichment medium (composition: fine powder chitin 2.5g, MgSO of sterilizing4·7H2O 0.5g,
K2HPO40.7g, KH2PO40.3g, FeSO4·7H2O 0.01g, distilled water 1000ml), subpackage 20ml in aseptic triangular flask, and
Taking 1.0g fresh soil sample and add in triangular flask, mixing, under room temperature, 150r/min cultivates 48h, obtains the culture fluid of enrichment.
(2) coating separates: drawing 1mL filtrate and add 9.0mL sterilized water, it is 10 that serial dilution obtains dilution factor for 3 times-4Sample
Product liquid, carries out gradient dilution, and soil dilution liquid 0.02mL coats on plate isolation base, cultivates 4-7d, observes, chooses for 28 DEG C
Take the bacterial isolates producing transparent circle.
(3) cultivation and purification: the single bacterium colony of picking setting-out purification on plate isolation base is cultivated, and purification obtains after cultivating
The bacterial strain arrived, as strains tested, is inoculated into the temporary transient preservation in beef extract-peptone inclined-plane.
(4) screening active ingredients: use the multiple screen method of shaking flask to carry out screening active ingredients, the bacterial strain separated is accessed (training in culture medium
Foster based component: fine powder chitin 10g, peptone 5g, yeast extract 5g, K2HPO40.7g, KH2PO40.3g, MgSO4·7H2O
0.5g, FeSO4·7H2O 0.01g, ZnSO40.01g, distilled water 1000ml), 28 DEG C, 150r/min shaken cultivation 3-4d, fermentation
Liquid is centrifugal obtains crude enzyme liquid, measures enzyme activity, then chooses the bacterial strain producing enzyme activity high and carries out multiple sieve (Fig. 1).Multiple screen method is same
On, measure enzyme activity, choose further and produce the bacterial strain that enzyme activity is high.
(5) activity assay: with the antibacterial of isolated and purified high yield chitinase, be target for examination pathogen, use flat
Plate opposite culture method directly measures the bacterial strain suppression ratio to pathogenic fungi.Method is first to be activated by bacterial strain to be screened, puts down at PDA
Pathogenic fungi is first inoculated at plate center, makes bacterium cake with the card punch of diameter 5mm in the punching of pathogen colony edge, and receives PDA
Flat board central authorities, then inoculate bacterial isolates to be measured, three weights of every strain antibacterial in culture dish surrounding away from pathogen about 2.5cm
Multiple, cultivate one week for 28 DEG C, see whether to produce inhibition zone, measure pathogenic fungi colony diameter by decussation method, calculate suppression
Rate.
(6) determination of classification position: extract the DNA of strains tested, carries out 16SrRNA order-checking and determines that bacterial strain is taxonomically
Position.
(7) select the strain bacterial strain (WXX1) that antibacterial effect is best, and amplification culture by above testing sieve, extract it
DNA, determines classification position by 16SrNA cloning and sequencing, and this bacterial strain is under the jurisdiction of Proteobacteria-γ and deforms Gammaproteobacteria-pseudomonas
Mesh-pseudomonadaceae-Rhodopseudomonas.And in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms,
The preservation time is on October 26th, 2015, and deposit number is CGMCC No.11539.
Preserving number is: the biological property of the bacterial strain of CGMCC NO.11539 is: this bacterium is gram negative bacteria, aerobic
Type, shaft-like, micro-Huang, the magnitude range of antibacterial: 1.5-3 μm；This bacterium is best suitable for growth temperature and is 28-30 DEG C, and pH is 5-7.
Embodiment 2: the bacteriostatic experiment to various soil-borne fungus type pathogen
The bacterial strain that deposit number is CGMCC NO.11539 is utilized to carry out antibacterial experiment: first to be activated by described bacterial strain,
Pathogenic fungi is first inoculated at PDA plate center, makes bacterium cake with the card punch of diameter 5mm in the punching of pathogen colony edge, and connects
To PDA plate central authorities, then inoculate bacterial isolates to be measured, every strain antibacterial three in culture dish surrounding away from pathogen about 2.5cm
Individual repetition, cultivates one week for 28 DEG C, sees whether to produce inhibition zone, measures pathogenic fungi colony diameter by decussation method, calculates
Suppression ratio.Selected pathogen is: Rhizoctonia solani Kuhn (Rhizoctonia solani), Fusarium solani (Fusarium
Solani), Fusarium oxysporum (Fusarium oxysporum f.sp.diath), black and white Verticillium (Verticillium
Dahliae Kleb), such as Fig. 2 Fig. 5, find that described bacterial strain is respectively provided with good bacteriostasis to above-mentioned soil-borne disease fungal pathogens
(table 1).Then by scanning electron microscope, fungal mycelia being carried out microexamination discovery, hyphal cell protoplasm shrinks, cavity occurs；
Mycelia deformity enlarges into spherical；Hyphal cell wall is damaged, clear up, protoplasm excessive (Fig. 6).
The mensuration of the in vitro bacteriostasis rate of table 1 bacterial strain
Embodiment 3: greenhouse live body preventing and treating experiment
PDA culture medium activates and at Roots of Peanut corruption pathogen (Fusarium solani) 28 DEG C, cultivates 7d, with sterilized water respectively
Rinsing pathogen spore in culture medium, bacterium solution is with sterilized filter paper filtering to remove mycelia, and filtrate is added suitably steaming by pathogen
It is 1 × 10 that distilled water is diluted to spore concentration7The suspension of individual/mL, standby.
To add for examination biocontrol bacterial strain and carry out liquid without fungal mycelia inductor culture fluid (Carnis Bovis seu Bubali cream 3%, peptone 8%)
Body fermentation (26 DEG C, 72h, 200rpm), the most again to inoculate the pig manure substrate become thoroughly decomposed in 5% ratio, places calorstat (28
DEG C) cultivate 5d, standby as microbial inoculum A.
The most in same way as described above, liquid medium within simply adds inactivation fungal mycelia inductor (3%) to carry out
Antibacterial strengthening liquid fermentation, the microbial inoculum B of preparation is standby.
The Upland Red Soil agricultural land soil gathered, after pulverizing and sieving, and 121 DEG C, moist heat sterilization 3h, to prepare sterile soil.Press
5% ratio is added pathogen spore liquid and is mixed thoroughly with sterile soil, fills basin.Then in 1:2 ratio, peanut seed and microbial inoculum A/B are carried out
Mixing, stand-by.
Test and set 4 process altogether:
CK1, sterile soil, without pathogen and microbial inoculum；
CK2, adds pathogen, it is thus achieved that band soil bacteria；
A, containing pathogen soil+microbial inoculum A；
B, containing pathogen soil+microbial inoculum B.
Often processing 10 basins, after plantation, regular watering observes incidence.30d " Invest, Then Investigate " Semen arachidis hypogaeae root occurring degree.Semen arachidis hypogaeae
Root rot disease scale standard: 0 grade, without Visual symptoms；1 grade, black rotten area accounts for less than the 5% of whole main root area；2 grades, black
Rotten area accounts for whole main root area 5%～25%；3 grades, black rotten area accounts for the 25%～50% of whole main root area；4 grades, black corruption
Area accounts for the 50%～75% of whole main root area；5 grades, black rotten area accounts for more than the 75% of whole main root area.
The table 2 bacterial strain WXX1 difference microbial inoculum type of process prevention effect to soil-borne disease
| Process type | Disease index | Prevention effect (compared with CK2) |
| CK1 | 0 | / |
| CK2 | 63.5 | 0 |
| A | 34.4 | 45.8% |
| B | 21.2 | 66.7% |
Different microbial inoculums peanut growth is all had good facilitation, plant height and well developed root system degree be all remarkably higher than right
According to group.Control effect testing result shows, 2 kinds of microbial inoculums of preparation are respectively 45.8% for the live body preventive effect of Roots of Peanut maize ear rot,
66.7%, wherein microbial inoculum B is best to the preventive effect of soil-borne disease bacterium, significantly reduces rotten brown stain, preventive effect highly significant.
Embodiment 4: field Peanut continuous cropping ground experiment
The bacterial strain that deposit number is CGMCC NO.11539 transfers to Peanut continuous cropping field test, and continuous 3 years in field certainly
So verify the inhibition that Peanut continuous cropping soil-borne fungus sexually transmitted disease (STD) is done harm to by microbial inoculum prepared by this bacterial strain under cultivation condition, specific as follows:
(1) for examination soil basic condition: test arrangement is in the continuous cropping obstacle in Upland Red Soil peanut cultivation district, Yujiang County, Jiangxi
Soil, trial zone long-term root-rot sickness rate is at 40%-70%, and constantly rises with continuous cropping time limit prolongation sickness rate, some sections
Soil can not be cultivated, and sickness rate is up to more than 80%.The region continuous cropping red soil Semen arachidis hypogaeae field of 5 years has been selected in test in 2012
Carry out compliance test result, within 2013, selected the Semen arachidis hypogaeae field of 7 years of continuous cropping to carry out compliance test result, within 2014, selected continuous cropping 4
The Semen arachidis hypogaeae field in year carries out compliance test result, is respectively provided with 4 groups of contrast tests every year, in addition to test processes difference, keeps trial zone
Edaphic condition and cultivation management technology are unified.
(2) test process and method: test She12Ge community, each zone leader 10m altogether, sets 1m ditch between wide 5m community
Away from, group arrangement Semen arachidis hypogaeae spacing in the rows in random district is 18-20cm, and line-spacing is 45-50cm, 2 seeds of every bunch planting.If 4 groups of experiments, respectively:
CK: conventional NPK fertilising, carbamide 150kg hm executed by base prior to seeding-2, potassium chloride 225kg hm-2, calcium and magnesium phosphorus
1125kg hm-2；
A: microbial inoculum A and peanut seed with preparing without mycelia inductor mix thoroughly (2:1), conventional NPK dose with CK at
Reason；
B: the microbial inoculum B prepared with the inductor containing mycelia mixes thoroughly (2:1) with peanut seed, and conventional NPK dose is with CK process；
C:50% carbendazol wettable powder 800 times, conventional NPK dose is with CK process.Each process sets 3 repetitions.
(3) result of the test: the efficiency test result to different Peanut continuous cropping fields showed (table 3) in continuous 3 years: WXX1 biological and ecological methods to prevent plant disease, pests, and erosion
Microbial inoculum is respectively provided with preferable prevention effect (Fig. 7) to the silborne fungal diseases in Peanut continuous cropping ground, and wherein the present invention prepares microbial inoculum B
Preventive effect the highest, the preventive effect of Semen arachidis hypogaeae damping-off, root rot and southern blight is respectively 53.0～60.7%, 53.2～58.8%,
65.0～66.7%, its effect is suitable with 50% carbendazol wettable powder preventive effect, but effect of increasing production is the most notable.Therefore, originally
Microbial inoculum prepared by the invention bacterial strain with deposit number as CGMCCNO.11539 is to suppression red soil Peanut continuous cropping soil-borne fungus sexually transmitted disease (STD) evil
Generation and spread there is preferable action and efficacy, and prevention effect is stable, is the biology of South Red Soil Region Semen arachidis hypogaeae continuous cropping obstacle
Repair product.
The field efficacy analysis (2012 of table 3WXX1 biocontrol microorganisms difference microbial inoculum preventing and treating continuous cropping soil Semen arachidis hypogaeae silborne fungal diseases
Year)
The field efficacy analysis (2013 of table 4WXX1 biocontrol microorganisms difference microbial inoculum preventing and treating continuous cropping soil Semen arachidis hypogaeae silborne fungal diseases
Year)
The field efficacy analysis (2014 of table 5WXX1 biocontrol microorganisms difference microbial inoculum preventing and treating continuous cropping soil Semen arachidis hypogaeae silborne fungal diseases
Year)
Claims (7)
1. pseudomonas (Pseudomonas protegens) WXX-1, deposit number is CGMCC No.11539.
2. the application in preventing and treating soil-borne fungus sexually transmitted disease (STD) evil of the bacterial strain described in claim 1.
3. the application in preventing and treating Peanut continuous cropping soil-borne fungus sexually transmitted disease (STD) evil of the bacterial strain described in claim 1.
4. the microbial inoculum preventing and treating soil-borne fungus sexually transmitted disease (STD) evil, it is characterised in that: by the pseudomonas WXX-1 described in claim 1
Prepare.
5. microbial inoculum as claimed in claim 4, it is characterised in that in this microbial inoculum, pseudomonas WXX-1 induces through fungal mycelium
Strengthening, effectively survives in the organic manure become thoroughly decomposed, and the content of WXX-1 is 1 × 109More than cfu/g.
6. the application in preparation preventing and treating soil-borne fungus sexually transmitted disease (STD) evil microbial inoculum of the bacterial strain described in claim 1.
7. the application process of microbial inoculum as described in claim 4 or 5, it is characterised in that by weight 1:2 ratio by peanut seed with
Microbial inoculum is puddled, microbial inoculum consumption 20-30 kg/acre；After field trench digging, it is sowed in advance together with the peanut seed after mixing thoroughly with microbial inoculum
In the seed furrow held successfully, then earthing.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109757329A (en) * | 2019-02-20 | 2019-05-17 | 山东省农业科学院生物技术研究中心 | A method of improving Peanut continuous cropping yield and quality |
| CN110622825A (en) * | 2019-10-28 | 2019-12-31 | 湖南省烟草公司永州市公司 | Tobacco seedling culture medium and preparation method and application thereof |
| CN110643538A (en) * | 2019-10-15 | 2020-01-03 | 浙江大学 | Pseudomonas fluorescens XY2F4 and application thereof in preventing and treating crop verticillium wilt |
| CN114045232A (en) * | 2021-10-29 | 2022-02-15 | 领先生物农业股份有限公司 | Organic material rapid fermentation synergistic microbial inoculum and application thereof |
| CN116235867A (en) * | 2022-11-30 | 2023-06-09 | 乐施康农业科技有限公司 | Pathogen for preventing and treating cotton wilt and preparation method thereof |
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| 甄雪冰: "土传病害生防菌株的筛选鉴定及防治效果研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 陈荣庚: "木霉菌的分离鉴定及其抑菌机理研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
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| CN110643538B (en) * | 2019-10-15 | 2021-04-27 | 浙江大学 | Pseudomonas fluorescens XY2F4 and application thereof in preventing and treating crop verticillium wilt |
| CN110622825A (en) * | 2019-10-28 | 2019-12-31 | 湖南省烟草公司永州市公司 | Tobacco seedling culture medium and preparation method and application thereof |
| CN114045232A (en) * | 2021-10-29 | 2022-02-15 | 领先生物农业股份有限公司 | Organic material rapid fermentation synergistic microbial inoculum and application thereof |
| CN114045232B (en) * | 2021-10-29 | 2023-07-18 | 领先生物农业股份有限公司 | Organic material rapid fermentation synergistic microbial agent and application thereof |
| CN116235867A (en) * | 2022-11-30 | 2023-06-09 | 乐施康农业科技有限公司 | Pathogen for preventing and treating cotton wilt and preparation method thereof |
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