CN106119154B - It is a kind of prevent and treat Peanut continuous cropping silborne fungal diseases bacterial strain and application - Google Patents
It is a kind of prevent and treat Peanut continuous cropping silborne fungal diseases bacterial strain and application Download PDFInfo
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- CN106119154B CN106119154B CN201610475283.4A CN201610475283A CN106119154B CN 106119154 B CN106119154 B CN 106119154B CN 201610475283 A CN201610475283 A CN 201610475283A CN 106119154 B CN106119154 B CN 106119154B
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- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
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- C12R2001/38—Pseudomonas
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12N1/205—Bacterial isolates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The present invention relates to the bacterium bacterial strain and its microbial inoculum of a kind of prevention and treatment Peanut continuous cropping soil fungi venereal disease evil, the bacterial strain is a kind of pseudomonad (Pseudomonas protegens), and deposit number is CGMCC No.11539.Bacterial strain 16s rRNA base sequence is as shown in SEQ ID No:1.Bacterial strain production chitinase (degradation pathogen hyphal cell wall) activity of the invention is higher, has significant degradation capability to a variety of soil-borne fungus venereal disease opportunistic pathogen mycelium such as sickle-like bacteria, rhizoctonia, Verticillium dahliae.Microbial inoculum of the present invention is dressed seed generally only 20-30 kgs/acre of dosage, the soil facsimile disease that nonirrigated farmland Peanut continuous cropping root rot, damping-off, southern blight etc. take place frequently can be effectively prevented, preventive effect is up to 55% or more, have many advantages, such as that dosage is small, antimicrobial spectrum is wide, obvious effect of increasing production, effectively mitigates influence of the soil-borne disease to continuous cropping tibet milkwort root production loss.
Description
Technical field
The present invention relates to the bacterial strains and its bacterial preparation process etc. for Peanut continuous cropping soil facsimile disease, belong to agricultural
Intensive manufacture technical field is exclusively used in overcoming the problems, such as that Peanut continuous cropping silborne fungal diseases are high-incidence.
Background technique
Peanut is the important oil crops in China, and long-term sown area accounts for 35% or so of the total sown area of oil crops.
Steep hilly region in southern China is one of the peanut main producing region in China, opposite compared with the main producing region of North China, South Red Soil Region peanut yield
Lower (average product be only North China producing region 60% or so), this was both related with peanut varieties, weather conditions, soil fertility etc.,
Also seriously there is certain relationship with South Red Soil Region peanut continuous cropping obstacle.Since red soil slopeland fertility is lower, seasonal drought is tight
Weight, peanut continuous cropping phenomenon are universal.Peanut continuous cropping continuous cropping often leads to soil facsimile disease aggravation, increases with the continuous cropping time limit, flower
Maize ear rot of taking root disease incidence rises at double, and damping-off and southern blight persistently increase.Therefore, continuous cropping tibet milkwort root soil-borne fungus is effectively prevented
Venereal disease evil becomes the urgent current demand of the sustainable production of peanut.
Currently used administering method has the biological organic fertilizer of application chemical bactericide and application containing biocontrol microorganisms.Chemical bacterium
Agent or efficient fumigant can effectively kill soil surface characters radix, temporarily mitigate disease, but also inhibit soil simultaneously
Beneficial bacterium, in the long run/term, chemical sterilization will further deteriorate soil ecology function, be unfavorable for agricultural production.Biological and ecological methods to prevent plant disease, pests, and erosion
The screening of bacterium is the key that biological organic fertilizer preparation, and the screening of biocontrol agent at present is mainly from growth-promoting, metabolite biocidal property
Angle is carried out, and is generallyd use and used with after organic fertilizer progress mixed fermentation.It but is a kind of living body biological in view of biocontrol microorganisms, no
It is difficult directly to cause field control effect unstable in organic fertilizer continued survival etc. by strengthening induction.In addition, existing at present
The general dosage of biological control organic fertilizer larger (> 200 kgs/acre), application cost are higher.
Under normal circumstances, continuous cropping soil-borne disease fungal pathogens abundance it is relatively high (> 30%), the cell wall of these disease fungus
It is by there is chitin to constitute.
Summary of the invention
It is an object of the invention to solve the shortcomings of the prior art, one kind is provided and is caused for preventing and treating by disease fungus
Peanut soil-borne disease the effective preparation method and applications of biocontrol agent, so that Peanut continuous cropping rhizosphere disease fungus be effectively reduced
Radix achievees the purpose that inhibition soil-borne pathogen infecting peanut root, and the method for the present invention specialization is strong, effect stability.
To achieve the above object, the invention adopts the following technical scheme:
A kind of pseudomonad (Pseudomonas protegens) WXX-1, deposit number are CGMCC No.11539.Institute
Stating bacterial strain is gram-negative bacterium, and the 16s rDNA base sequence of bacterial strain is as shown in SEQ ID No:1.
The invention also discloses application of the bacterial strain in prevention and treatment soil facsimile disease.Especially in prevention and treatment red soil
Application in Peanut continuous cropping soil facsimile disease.
The present invention also provides application of the bacterial strain in preparation prevention and treatment soil facsimile disease microbial inoculum.
A kind of microbial inoculum for preventing and treating soil facsimile disease, the microbial inoculum are made by pseudomonad WXX-1.
Further, pseudomonad WXX-1 is induced through fungal mycelium and is strengthened in the microbial inoculum, and effectively surviving in decomposed has
In machine muck, the content of WXX-1 is 1 × 109Cfu/g or more.Microbial inoculum field strong applicability may be directly applied to prevention and treatment southern red
Earth Peanut continuous cropping soil facsimile disease.
The present invention provides pseudomonad WXX1 microbial inoculum technology of preparing are as follows:
1) inactivation saprophytic fungus mycelia inductor obtains: configuration PD culture solution (potato 20% (g/ml), glucose
1.5% (g/ml), distilled water 1000ml), and sterilization treatment；Then it is inoculated with Mortierella (Mortierella sp.), is placed in constant temperature
Shaking table dark culturing (28 DEG C, 120rpm), after 7d, filter paper filtering obtains mycelia, distills water washing 2 times, high-temperature sterilization after suction filtration
(121 DEG C, 20min) are spare.
2) biocidal property enriched medium: beef extract 3% (g/ml) is configured, peptone 8% (g/ml) inactivates saprophytic fungus bacterium
Filament 3% (g/ml)；
3) strain stability is strengthened: the bacterial strain being added in above-mentioned culture medium, ferment ventilatory capacity 10L/m, speed of agitator
200r/min, with salt acid for adjusting pH value 5.5-6.5, fermentation temperature is 26 DEG C, fermentation time 72h, and measuring bacterium solution OD value is 1.0 left
It is right；
4) using decomposed pig manure as matrix, above-mentioned WXX1 biocidal property forced fermentation liquid the microbial inoculum preparation: is pressed 5%
Ratio is inoculated into above-mentioned matrix, places (28 DEG C) culture 5d of insulating box；By second step to the bacterial strain to hypha,hyphae parasitism
Reinforcing, the microbial inoculum can colonize efficiently in the organic substrate containing a large amount of saprophytic fungus, the bacterium amount reaches 1 ×
109cfu/g.Finally obtain the solid mix bacterium agent that WXX1 bacteriostasis property is strengthened.
The method of administration of microbial inoculum of the present invention is to puddle peanut seed and microbial inoculum in 1:2 ratio (weight ratio), microbial inoculum
20-30 kgs/acre of dosage；After field trench digging, the seed furrow held successfully in advance will be sowed at together with the peanut seed after mixing thoroughly with microbial inoculum
In, then earthing.On the one hand the bacterial strain can be colonized effectively in peanut rhizosphere in this way, on the other hand can be effectively attached in Roots of Peanut circle
It is close to form protecting wall, achieve the purpose that inhibit soil-borne pathogen infecting peanut root.The present invention can effectively prevent continuous cropping tibet milkwort root
Root rot, damping-off, base rot disease, southern blight, preventive effect have field control effect stabilization, antimicrobial spectrum wide up to 55% or more.
The invention has the advantages that bacterial strain WXX1 production chitinase (degradation pathogen hyphal cell wall) activity is higher, to sickle
A variety of soil-borne fungus venereal disease opportunistic pathogen mycelium such as knife bacterium, rhizoctonia, Verticillium dahliae have significant degradation capability, can by strengthening induction
With existence parasitic on quasi-microorganism saprophytic effectively in organic fertilizer；It is inoculated into decomposed organic manure and is prepared into solid fungicide；It is
For preventing and treating the biocontrol bacteria of Peanut continuous cropping soil facsimile disease, microbial inoculum dosage is few, at low cost, obvious effect of increasing production.Soil passes
Fungal disease is the Major Diseases occurred in intensive agriculture production, can cause crop withered death, the underproduction is serious.If pressing
The microbial inoculum of above method preparation applies Peanut continuous cropping rhizosphere, so that it may stablize existence within the peanut growth phase, to be effectively formed suppression
The protecting wall of pathogen infection processed, and peanut rhizosphere pathogen radix is effectively reduced, finally reached with " competition " between microorganism
To the purpose for effectively preventing Peanut continuous cropping silborne fungal diseases.
Detailed description of the invention
Fig. 1 is growth and transparent circle of the pseudomonad WXX1 on culture medium (chitin containing 2%).
Fig. 2 is experiment effect figure of the pseudomonad WXX1 to Fusarium oxysporum.
Note: 1 is Fusarium oxysporum in figure, and 2 be pseudomonad WXX1
Fig. 3 is experiment effect figure of the pseudomonad WXX1 to Fusarium solani.
Note: 1 is Fusarium solani in figure, and 2 be pseudomonad WXX1
Fig. 4 is the experiment effect figure of pseudomonad WXX1 Rhizoctonia solani.
Note: 1 is Rhizoctonia solani Kuhn in figure, and 2 be pseudomonad WXX1
Fig. 5 is experiment effect figure of the pseudomonad WXX1 to Verticillium dahliae.
Note: 1 is Verticillium dahliae in figure, and 2 be pseudomonad WXX1
Fig. 6 is the electron microscope that pseudomonad WXX1 destroys pathogen hypha,hyphae.
Fig. 7 is pseudomonad WXX1 microbial inoculum Field information effect picture.
Bacterial strain WXX-1 in the present invention, the classification naming of the bacterial strain are as follows: pseudomonad (Pseudomonas
Protegens), deposit number is CGMCC No.11539, and depositary institution is that China Committee for Culture Collection of Microorganisms is general
Logical microorganism center (CGMCC), depositary institution address are as follows: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the Chinese Academy of Sciences are micro-
Biological study institute, the deposit date is on October 26th, 2015.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.
Embodiment 1:
The peanut rhizosphere soil for acquiring one piece of red earth area Peanut continuous cropping ground carries out relevant test, the specific steps are that:
(1) soil sample is handled: preparing the enriched medium (composition: fine powder chitin 2.5g, MgSO of sterilizing4·7H2O 0.5g,
K2HPO40.7g, KH2PO40.3g, FeSO4·7H2O 0.01g, distilled water 1000ml), 20ml is dispensed in sterile triangular flask, and
It takes 1.0g fresh soil sample to be added in triangular flask, mixes, 150r/min cultivates 48h, the culture solution being enriched at room temperature.
(2) it coating separation: draws 1mL filtrate and 9.0mL sterile water is added, 3 acquisition dilutions of serial dilution are 10-4Sample
Product liquid carries out gradient dilution, and soil dilution liquid 0.02mL is coated on plate isolation base, and 28 DEG C of culture 4-7d are observed, chosen
Take the bacterium bacterial strain for producing transparent circle.
(3) culture and purifying: picking single bacterium colony draws lines on plate isolation base purifies culture, after purifying culture
The bacterial strain arrived is inoculated into the temporary preservation in beef extract-peptone inclined-plane as strains tested.
(4) screening active ingredients: carrying out screening active ingredients using shaking flask secondary screening method, by (training on isolated bacterial strain access culture medium
Support based component: fine powder chitin 10g, peptone 5g, yeast extract 5g, K2HPO40.7g, KH2PO40.3g, MgSO4·7H2O
0.5g, FeSO4·7H2O 0.01g, ZnSO40.01g, distilled water 1000ml), 28 DEG C, 150r/min shaken cultivation 3-4d, fermentation
Liquid is centrifuged to obtain crude enzyme liquid, measures enzyme activity, then chooses the high bacterial strain of producing enzyme vigor and carries out secondary screening (Fig. 1).Secondary screening method is same
On, enzyme activity is measured, the high bacterial strain of producing enzyme vigor is further chosen.
(5) activity assay: being target for trying pathogen, using flat with the bacterium of the high yield chitinase isolated and purified
Plate opposite culture method directly measures bacterial strain to the inhibiting rate of disease fungus.Method is first to activate bacterial strain to be screened, flat in PDA
Plate center is first inoculated with disease fungus, bacteria cake is made in the punching of pathogen colony edge with the punch of diameter 5mm, and be connected to PDA
Then plate center is inoculated with bacterium bacterial strain to be measured, three weights of every plant of bacterium away from pathogen 2.5cm or so in culture dish surrounding
Multiple, 28 DEG C are cultivated one week, see whether to generate inhibition zone, are measured disease fungus colony diameter with crossing method, are calculated and inhibit
Rate.
(6) determination of classification position: extracting the DNA of strains tested, carries out 16SrRNA and determining bacterial strain is sequenced taxonomically
Position.
(7) one plant of best bacterial strain (WXX1) of antibacterial effect is selected by the above testing sieve, and expands culture, extract it
DNA has determined classification position by 16SrNA cloning and sequencing, which is under the jurisdiction of Proteobacteria-γ deformation Gammaproteobacteria-pseudomonad
Mesh-pseudomonadaceae-pseudomonas.And in China Committee for Culture Collection of Microorganisms's common micro-organisms center's preservation,
The preservation time is on October 26th, 2015, and deposit number is CGMCC No.11539.
Deposit number are as follows: the biological property of the bacterial strain of CGMCC NO.11539 are as follows: the bacterium is gramnegative bacterium, aerobic
Type, rod-shaped, micro- Huang, the magnitude range of bacterium: 1.5-3 μm；It is 28-30 DEG C that the bacterium, which is most suitable for growth temperature, pH 5-7.
Embodiment 2: to the bacteriostatic experiment of various soil-borne fungus type pathogens
Antibacterial experiment is carried out using the bacterial strain that deposit number is CGMCC NO.11539: first activating the bacterial strain,
PDA plate center is first inoculated with disease fungus, bacteria cake is made in the punching of pathogen colony edge with the punch of diameter 5mm, and connect
To PDA plate center, bacterium bacterial strain to be measured, every plant of bacterium three then are inoculated with away from pathogen 2.5cm or so in culture dish surrounding
A repetition, 28 DEG C are cultivated one week, see whether to generate inhibition zone, are measured disease fungus colony diameter with crossing method, are calculated
Inhibiting rate.Selected pathogen are as follows: Rhizoctonia solani Kuhn (Rhizoctonia solani), Fusarium solani (Fusarium
Solani), Fusarium oxysporum (Fusarium oxysporum f.sp.diath), verticilliumalbo-atrum (Verticillium
Dahliae Kleb), such as Fig. 2-Fig. 5, it is found that the bacterial strain all has good bacteriostasis to above-mentioned soil-borne disease fungal pathogens
(table 1).Then microexamination discovery is carried out to hypha,hyphae by scanning electron microscope, hyphal cell plasm is shunk, and vacuole occurs；
Mycelia deformity expands glomeration；Hyphal cell wall is damaged, clears up, and plasm is excessive (Fig. 6).
The measurement of the in vitro bacteriostasis rate of 1 bacterial strain of table
Embodiment 3: greenhouse living body prevention and treatment experiment
7d is cultivated at 28 DEG C of activation peanut root rot opportunistic pathogen (Fusarium solani) in PDA culture medium, is distinguished with sterile water
Pathogen spore on culture medium is rinsed, bacterium solution is filtered with sterilized filter paper to remove mycelia, and filtrate is added appropriate steaming by pathogen
It is 1 × 10 that distilled water, which is diluted to spore concentration,7The suspension of a/mL, it is spare.
It will be added for examination biocontrol bacterial strain and carry out liquid without hypha,hyphae inductor culture solution (beef extract 3%, peptone 8%)
Body ferments (26 DEG C, 72h, 200rpm), then places insulating box (28 again to be inoculated with decomposed pig manure matrix in 5% ratio
DEG C) 5d is cultivated, it is spare as microbial inoculum A.
Simultaneously in same way as described above, only addition inactivation hypha,hyphae inductor (3%) carries out in liquid medium
The microbial inoculum B of antibacterial reinforcing liquid fermentation, preparation is spare.
The Upland Red Soil agricultural land soil of acquisition, after pulverizing and sieving, and 121 DEG C, moist heat sterilization 3h, to prepare sterile soil.It presses
5% ratio addition pathogen spore liquid is mixed thoroughly with sterile soil, fills basin.Then peanut seed and microbial inoculum A/B are carried out in 1:2 ratio
It mixes, for use.
Experiment sets 4 processing altogether:
CK1, sterile soil are free of pathogen and microbial inoculum；
CK2 adds pathogen, obtains band soil bacteria；
A, soil containing pathogen+microbial inoculum A；
B, soil containing pathogen+microbial inoculum B.
10 basins of every processing, regular watering observes incidence after plantation.Peanut root occurring degree is investigated after 30d.Peanut
Root rot disease scale standard: 0 grade, no Visual symptoms；1 grade, black corruption area accounts for the 5% or less of entire main root area；It is 2 grades, black
Rotten area accounts for entire main root area 5%~25%；3 grades, black corruption area accounts for the 25%~50% of entire main root area；4 grades, black corruption
Area accounts for the 50%~75% of entire main root area；5 grades, black corruption area accounts for 75% or more of entire main root area.
Control efficiency of 2 bacterial strain WXX1 difference microbial inoculum type of the table processing to soil-borne disease
| Handle type | Disease index | Control efficiency (compared with CK2) |
| CK1 | 0 | / |
| CK2 | 63.5 | 0 |
| A | 34.4 | 45.8% |
| B | 21.2 | 66.7% |
Different microbial inoculums all have good facilitation to peanut growth, and plant height and well developed root system degree are all remarkably higher than pair
According to group.Control effect testing the result shows that, 2 kinds of microbial inoculums of preparation for the living body preventive effect of peanut root rot are respectively 45.8%,
66.7%, wherein microbial inoculum B is best to the preventive effect of soil-borne disease bacterium, significantly reduces rotten brown stain, preventive effect highly significant.
Embodiment 4: it tests to field Peanut continuous cropping
The bacterial strain that deposit number is CGMCC NO.11539 is switched into Peanut continuous cropping field trial, continuous 3 years field from
Inhibitory effect of the microbial inoculum to Peanut continuous cropping soil facsimile disease of bacterial strain preparation is verified under right cultivation condition, specific as follows:
(1) for trying soil basic condition: test arrangement is in the continuous cropping obstacle of Jiangxi Yujiang County Upland Red Soil peanut species growing area
Soil, the long-term root-rot disease incidence in trial zone extend disease incidence with the continuous cropping time limit and constantly rise in 40%-70%, some sections
Soil cannot cultivate, and disease incidence is up to 80% or more.Region continuous cropping 5 years red soil peanut fields have been selected in test in 2012
Compliance test result is carried out, the peanut field selected for 2013 continuous cropping 7 years carries out compliance test result, has selected continuous cropping 4 within 2014
The peanut field in year carries out compliance test result, and 4 groups of comparative tests are respectively set every year, in addition to test process difference, keeps trial zone
Edaphic condition and cultivation management technology are unified.
(2) test process and method: testing and set 12 cells altogether, each zone leader 10m, sets 1m ditch between wide 5m cell
Away from random district's groups arrangement peanut spacing in the rows is 18-20cm, line-spacing 45-50cm, 2 seeds of every bunch planting.If 4 groups of experiments, respectively:
CK: conventional NPK fertilising, base applies urea 150kg hm prior to seeding-2, potassium chloride 225kg hm-2, calcium and magnesium phosphorus
1125kg hm-2；
A: with without mycelia inductor preparation microbial inoculum A and peanut seed mix thoroughly (2:1), conventional NPK dose with CK at
Reason；
B: the microbial inoculum B and peanut seed prepared with inductor containing mycelia is mixed thoroughly (2:1), and conventional NPK dose is the same as CK processing；
800 times of C:50% carbendazol wettable powder, conventional NPK dose is the same as CK processing.Each processing sets 3 repetitions.
(3) test result: the continuous 3 years efficiency tests to different Peanut continuous cropping fields are the result shows that (table 3): WXX1 biological and ecological methods to prevent plant disease, pests, and erosion
Microbial inoculum all has preferable control efficiency (Fig. 7) to the silborne fungal diseases in Peanut continuous cropping ground, and wherein the present invention prepares microbial inoculum B
Preventive effect highest, the preventive effect to peanut damping-off, root rot and southern blight is respectively 53.0~60.7%, 53.2~58.8%,
65.0~66.7%, effect is suitable with 50% carbendazol wettable powder preventive effect, but effect of increasing production is more significant.Therefore, originally
Microbial inoculum prepared by bacterial strain of the invention with deposit number for CGMCCNO.11539 is to inhibition red soil Peanut continuous cropping soil facsimile disease
Generation and sprawling have preferable action and efficacy, and control efficiency stablize, be the biology of South Red Soil Region peanut continuous cropping obstacle
Repair product.
Table 3WXX1 biocontrol microorganisms difference microbial inoculum prevents and treats the field efficacy analysis (2012 of continuous cropping soil peanut silborne fungal diseases
Year)
Table 4WXX1 biocontrol microorganisms difference microbial inoculum prevents and treats the field efficacy analysis (2013 of continuous cropping soil peanut silborne fungal diseases
Year)
Table 5WXX1 biocontrol microorganisms difference microbial inoculum prevents and treats the field efficacy analysis (2014 of continuous cropping soil peanut silborne fungal diseases
Year)
Claims (5)
1. a kind of pseudomonad (Pseudomonas protegens) WXX-1, deposit number is CGMCC No.11539；It is described
Bacterial strain be applied to prevention and treatment Peanut continuous cropping soil facsimile disease.
2. a kind of microbial inoculum for preventing and treating peanut soil facsimile disease, it is characterised in that: by pseudomonad described in claim 1
WXX-1 is made.
3. microbial inoculum as claimed in claim 2, which is characterized in that pseudomonad WXX-1 is induced through fungal mycelium in the microbial inoculum
Strengthen, effectively survive in decomposed organic manure, the content of WXX-1 is 1 × 109Cfu/g or more.
4. application of the bacterial strain described in claim 1 in preparation prevention and treatment peanut soil facsimile disease microbial inoculum.
5. the method for administration of microbial inoculum described in Claims 2 or 3, which is characterized in that by weight 1:2 ratio by peanut seed and bacterium
Agent is puddled, 20-30 kgs/acre of microbial inoculum dosage；After field trench digging, opened being sowed at together with the peanut seed after being mixed thoroughly with microbial inoculum in advance
In good seed furrow, then earthing.
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| CN110643538B (en) * | 2019-10-15 | 2021-04-27 | 浙江大学 | Pseudomonas fluorescens XY2F4 and application thereof in preventing and treating crop verticillium wilt |
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| CN114045232B (en) * | 2021-10-29 | 2023-07-18 | 领先生物农业股份有限公司 | Organic material rapid fermentation synergistic microbial agent and application thereof |
| CN116235867A (en) * | 2022-11-30 | 2023-06-09 | 乐施康农业科技有限公司 | Pathogen for preventing and treating cotton wilt and preparation method thereof |
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|---|---|
| CN106119154A (en) | 2016-11-16 |
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