CN103193547B - Environment-friendly plant source disease-resistant growth promoting agent and preparation method thereof - Google Patents
Environment-friendly plant source disease-resistant growth promoting agent and preparation method thereof Download PDFInfo
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- CN103193547B CN103193547B CN201310153364.9A CN201310153364A CN103193547B CN 103193547 B CN103193547 B CN 103193547B CN 201310153364 A CN201310153364 A CN 201310153364A CN 103193547 B CN103193547 B CN 103193547B
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Abstract
The invention relates to an environment-friendly plant source disease-resistant growth promoting agent and a preparation method of the environment-friendly plant source disease-resistant growth promoting agent. According to the plant source disease-resistant growth promoting agent, a dandelion extract, a ginkgo biloba extract and a burdock oligosaccharide-copper complex are used as main components and are mixed with monopotassium phosphate or potassium nitrate, magnesium sulfate, zinc sulfate, ferrous sulfate, boric acid and copper sulfate serving as large nutrient elements, medium nutrient elements and micronutrient elements; and the plant source disease-resistant growth promoting agent is prepared by compounding, chelating and fully stirring. Combining nutrition and disease resistance into a whole, the plant source disease-resistant growth promoting agent is wide in spectrum resistance; and by improving the immunity of a crop, the health of the plant is increased, the disease resistance of the plant is increased, pesticide application amount is reduced, the yield and the quality of the crop are improved, the performance is stable and the environment is friendly.
Description
Technical field
The present invention relates to a kind of environmentally friendly plant source disease-resistant growth-promoting agent and preparation method thereof, belong to plant stress-resistance growth-promoting technical field.
Background technology
The chemical pesticide produced in agriculture production is residual can cause toxic substance in water body, soil and agricultural-food to exceed standard, and seriously jeopardizes health and the value of farm production of the mankind.Therefore in the urgent need to development high-quality, non-harmful agricultural-food.And the safeguards of production high-quality, non-polluted farm product is healthy cultivation.Bio-control method is the primary selection of healthy cultivation, mainly comprises microorganism and builds biological control material, plant-sourced class biological control material and plant inducing anti-disease etc.Such as CN1946297A provides a kind of plant disease controlling agent or control material, and described control agent or control material comprise bacillus (Bacillus) bacterium that can suppress plant pathogenetic bacteria, fungi or virus infection or propagation.CN101433221A discloses a kind of traditional Chinese medicine bactericide of preventing and treating konjak soft rot, belongs to control of plant disease and field of biological pesticide.Its main ingredient and weight percent are: Bulbus Allii extract 12% ~ 36%, Rhizoma Coptidis extract 3% ~ 12%, Folium Lonicerae extract 3% ~ 10%, agricultural chemicals wettable powder agent aid 3% ~ 5%, and surplus is agricultural chemicals filling carrier.Above-mentioned each component is re-dubbed and to konjak soft rot pathogenic bacterium Erwinia Carotovora Pv. carotovora, there is the traditional Chinese medicine bactericide wettable powder showing sterilization effect, prevent and treat for konjak soft rot.This traditional Chinese medicine bactericide wettable powder environmental protection, test proves that its specificity is strong, and control konjak soft rot is better than chemical agent, and relative control effect, higher than 65%, is applied to during konjaku is produced and can improves Amorphophallus Yield.
A large number of experiments show that botanical pesticide and On Induced Disease Resistance In Plants have disease-resistant relative lasting, stable and often there is systematicness; Disease-resistant spectrum is wide, free of contamination feature.
Although botanical pesticide and On Induced Disease Resistance In Plants obtain the extensive recognition and acceptance of society, in current development research, there is the problem that formulation is single.Therefore be difficult to adapt to the demand of numerous control of plant disease, need to develop further and integrate nutrition, disease-resistant environmentally friendly plant source disease-resistant growth-promoting agent.
Summary of the invention
For the deficiencies in the prior art, the invention provides a kind of environmentally friendly plant source disease-resistant growth-promoting agent and preparation method thereof.
Technical scheme of the present invention is as follows:
Environmentally friendly plant source disease-resistant growth-promoting agent, feed composition weight part is as follows:
Herba Taraxaci extract 30 ~ 50 parts; Folium Ginkgo extract 20 ~ 40 parts; Burdock oligosaccharide-copper complex 1 ~ 2 part; Potassium primary phosphate or 5 ~ 10 parts, saltpetre; 0 ~ 2 part, magnesium sulfate; Manganous sulfate 0 ~ 5 part; 2 ~ 5 parts, zinc sulfate; 0 ~ 10 part, ferrous sulfate; Boric acid 1 ~ 10 part; 0 ~ 1 part, copper sulfate;
Described taraxacum stem and leaf extract Content of Chlorogenic Acid >=2%, flavonoid content >=1.5%; Alkaloid >=5.0% in described Folium Ginkgo extract, flavonoid content >=1.0%;
Described Herba Taraxaci extract is that lixiviate solid-to-liquid ratio is taraxacum dry powder by the filtrate of hot water extraction after the leaf of taraxacum and/or stem drying and crushing: water=1:25 ~ 30 weight ratio;
Described Semen Ginkgo extrac is by the filtrate of Ginkgo Leaf dry powder hot water extraction, and lixiviate solid-to-liquid ratio is Ginkgo Leaf dry powder: water=1:35 ~ 40 weight ratio.
Described burdock oligosaccharide-copper complex is formed by burdock oligosaccharide and copper sulfate complexing.
Above Herba Taraxaci extract and Semen Ginkgo extrac, be not particularly limited all by prior art extraction process by water.
According to the present invention, burdock oligosaccharide-copper complex formazan preparation is by existing burdock oligosaccharide complexes preparation technique.The present invention preferably provides following methods:
Described burdock oligosaccharide-copper complex is prepared according to the following steps: be dissolved in by burdock oligosaccharide in distilled water, copper-bath is joined in burdock oligosaccharide solution, adjust pH5.5 ~ 6.5, in 50 DEG C of stirred in water bath backflows 1 ~ 2 hour, concentrated, add acetone separate out precipitation, after filtration, washing, drying, recrystallization, vacuum-drying, obtain burdock oligosaccharide-copper complex.
In feed composition of the present invention, potassium primary phosphate or saltpetre are the source of macroelement K, and the middle and trace element such as Mg, Zn, Mn, Fe, Cu all select its vitriol form, and B selects boric acid; This macroelement and middle and trace element can be plant growth and provide nutrition.
Preferred according to the present invention, environmentally friendly plant source disease-resistant growth-promoting agent, feed composition weight part is as follows:
Taraxacum extracting solution 34 parts; Ginkgo extract 40 parts; Burdock oligosaccharide-copper complex 2 parts; 8 parts, saltpetre; 2 parts, magnesium sulfate; 3 parts, zinc sulfate; Boric acid 10 parts; 1 part, copper sulfate.This formula is the special disease-resistant growth-promoting agent of wheat.
Preferred according to the present invention, environmentally friendly plant source disease-resistant growth-promoting agent, feed composition weight part is as follows:
Taraxacum extracting solution 30 parts; Ginkgo extract 35 parts; Burdock oligosaccharide-copper complex 2 parts; Potassium primary phosphate 10 parts; 2 parts, magnesium sulfate; Manganous sulfate 3 parts; 5 parts, zinc sulfate; 3 parts, ferrous sulfate; Boric acid 10 parts; 1 part, copper sulfate.This formula is the special disease-resistant growth-promoting agent of tomato.
Preferred according to the present invention, environmentally friendly plant source disease-resistant growth-promoting agent, feed composition weight part is as follows:
Taraxacum extracting solution 37 parts; Ginkgo extract 25 parts; Burdock oligosaccharide-copper complex 2 parts; Potassium primary phosphate 8 parts; 2 parts, magnesium sulfate; 5 parts, zinc sulfate; Boric acid 10 parts; 1 part, copper sulfate.This formula is the special disease-resistant growth-promoting agent of corn.
Preferred according to the present invention, environmentally friendly plant source disease-resistant growth-promoting agent, feed composition weight part is as follows:
Herba Taraxaci extract 35 parts; Folium Ginkgo extract 30 parts; Burdock oligosaccharide-copper complex 2 parts; 10 parts, saltpetre; 2 parts, magnesium sulfate; 5 parts, zinc sulfate; 5 parts, ferrous sulfate; Boric acid 10 parts; 1 part, copper sulfate.This formula is the special disease-resistant growth-promoting agent of cucumber.
According to the present invention, the preparation method of environmentally friendly plant source disease-resistant growth-promoting agent, step is as follows:
(1) preparation of Herba Taraxaci extract, Folium Ginkgo extract
By hot water extraction after the leaf of taraxacum and/or stem drying and crushing, temperature 50 ~ 70 DEG C, lixiviate solid-to-liquid ratio is taraxacum dry powder: water=1:25 ~ 30 weight ratio, extraction time is 1 ~ 3 hour, vat liquor filters, chlorogenic acid content >=2% of gained stoste, flavonoid content >=1.5%; I.e. Herba Taraxaci extract;
By Ginkgo Leaf dry powder hot water extraction, temperature 50 ~ 70 DEG C, lixiviate solid-to-liquid ratio is Ginkgo Leaf dry powder: water=1:35 ~ 40 weight ratio, and extraction time is 1 ~ 3 hour, and vat liquor filters, alkaloid >=5.0% of gained stoste, flavonoid content >=1.0%; I.e. Folium Ginkgo extract;
(2) by proportioning, Herba Taraxaci extract obtained for step (1), Folium Ginkgo extract are added in reactor, thin up 20-30 times volume;
(3) by proportioning, in reactor, add medium trace element magnesium sulfate, manganous sulfate, zinc sulfate, ferrous sulfate, boric acid, copper sulfate, stirring and dissolving, then add chelator acid citrate or ethylenediamine tetraacetic acid (EDTA), be heated to 60 ~ 80 DEG C, adjust pH to 4.5 ~ 6.5, react 0.5 ~ 1 hour;
(4) after completion of the reaction, be cooled to 25 ~ 30 DEG C, in reactor, add burdock oligosaccharide-copper complex, potassium primary phosphate or saltpetre, dissolve, stirring is mixed evenly, and adjusts pH to 5.5 ~ 6.5; Filter, fully stir further, make mixed solution even.Obtain environmentally friendly plant source disease-resistant growth-promoting agent.
Preferably, as needs are preserved and long-distance transport for a long time, in above-mentioned environmentally friendly plant source disease-resistant growth-promoting agent, tensio-active agent can be added again, mix, canned, obtain environmentally friendly plant source disease-resistant growth-promoting agent product, for direct marketing.The consumption of tensio-active agent is 0.1 ~ 0.8wt% of feed composition total amount.
According to method of the present invention, preferably, the leaching process of step (1) completes in plant concentration extraction device.
According to method of the present invention, preferably, the chelator acid citrate consumption of step (3) is 5 ~ 15wt% of medium trace element consumption.
According to method of the present invention, preferably, the filtration of step (4) adopts vacuum filter.
According to method of the present invention, preferably, being fully stirred in liquid sand mill further of step (4) is carried out.
According to method of the present invention, in growth-promoting agent product, add tensio-active agent is stable and uniform in order to ensure product system, preferably, described tensio-active agent is selected from alkylphenol polyoxyethylene OP series or Span series, as OP-9,10,12, one of 20, Span-20,40,60, one of 80, further preferred described tensio-active agent is OP-10.
Compared with the prior art the present invention has the following advantages:
1, the present invention is with Folium Taraxacum and stem extracting solution, ginkgo biloba succi for solvent, and dissolve specific trace element needed for plant, the environmentally friendly plant source disease-resistant growth-promoting agent made, takes full advantage of the disease-resistant composition in extract, as alkaloid, chlorogenic acid etc.
2, the trace copper in plant materials with different target added in the present invention and burdock oligosaccharide are combined into burdock oligosaccharide-copper compound.Compared with single trace element or alone oligosaccharides, the induction of resistance of this material strengthens.
3, contain two class materials of sterilization and induction of resistance effect in this invention, compared with the product of current simple function composition, the anti-spectrum of this product is wide, and resistance against diseases strengthens, effect stability.
4, preparation method of the present invention, assembles herbal extraction equipment and Liquid Fertilizer production unit and uses, be convenient to plant extraction liquid and directly pump into reactor.
5, the nutritional characteristic of the present invention needed for crop and edaphic condition, be equipped with Different Nutrient Elements targetedly, develop and integrate nutrition, disease-resistant environmentally friendly plant source disease-resistant growth-promoting agent.The anti-spectrum of this disease-resistant growth-promoting agent is wide, by improving crop autoimmunity, promotes robust plant, strengthen the disease resistance of plant, reduce applications of pesticide amount, and then reach the object improving crop yield and quality, and stable performance, nontoxic to crop, harmless, not there is danger to human health and environment.
Accompanying drawing explanation
Fig. 1 environmentally friendly plant source disease-resistant growth-promoting agent production technological process.
The environmentally friendly plant source disease-resistant growth-promoting agent of Fig. 2 embodiment 1 is on the impact of wheat output.
The environmentally friendly plant source disease-resistant growth-promoting agent of Fig. 3 embodiment 2 is on the impact of tomato in plastic greenhouse output.
Embodiment
Below in conjunction with embodiment, the invention will be further described, but be not limited thereto.
In embodiment, described burdock oligosaccharide-copper complex is prepared according to the following steps: be dissolved in by burdock oligosaccharide 100g in 50mL distilled water, joins in the burdock oligosaccharide aqueous solution by water-soluble for copper sulfate 30g wiring solution-forming, adjust pH6, reflux 1 hour in 50 DEG C of stirred in water bath, concentrated, add acetone 15mL and separate out precipitation, filter, wash with water, dry, use anhydrous methanol recrystallization, vacuum-drying, obtains burdock oligosaccharide-copper complex.
In embodiment, equipment used is the existing conventional equipment in this area.Raw materials used all can market buy.
Embodiment 1: environmentally friendly plant source disease-resistant growth-promoting agent, feed composition weight part is as follows:
Taraxacum extracting solution 34 parts; Ginkgo extract 40 parts; Burdock oligosaccharide-copper complex 2 parts; 8 parts, saltpetre; 2 parts, magnesium sulfate; 3 parts, zinc sulfate; Boric acid 10 parts; 1 part, copper sulfate.This formula is specially adapted to wheat.
Preparation method, step is as follows:
(1) by hot water extraction after the leaf of taraxacum and/or stem drying and crushing, temperature 60 C, lixiviate solid-to-liquid ratio is taraxacum dry powder: water=1:30 weight ratio, and extraction time is 2.5 hours, and vat liquor filters, and obtains taraxacum extracting solution;
By Ginkgo Leaf dry powder hot water extraction, temperature 60 C, lixiviate solid-to-liquid ratio is Ginkgo Leaf dry powder: water=1:35 weight ratio, and extraction time is 2.5 hours, and vat liquor filters, and obtains ginkgo biloba succi;
(2) above-mentioned plant extraction liquid is input in reactor in proportion, thin up 20 times;
(3) in reactor, add magnesium sulfate, zinc sulfate, boric acid, copper sulfate in proportion, stirring and dissolving, then add chelator acid citrate 2.4 parts, heat 65 DEG C, adjust pH to 5.5, carry out chelatropic reaction, react 1 hour;
(4) after chelatropic reaction, be cooled to 25 ~ 30 DEG C, in reactor, add burdock oligosaccharide-copper complex and saltpetre, dissolve, stirring and evenly mixing;
(5) mixing solutions is filtered by vacuum filter, then enter liquid sand mill and stir further, make mixed solution even.
Further, mixing solutions is input to finished product still, add that Surfactant OP-10(accounts for feed composition total mass 0.5%), mix, canned, obtain wheat private environment friendly plant source disease-resistant growth-promoting agent product.
Embodiment 2: environmentally friendly plant source disease-resistant growth-promoting agent, feed composition weight part is as follows:
Taraxacum extracting solution 30 parts; Ginkgo extract 35 parts; Burdock oligosaccharide-copper complex 2 parts; Potassium primary phosphate 10 parts; 2 parts, magnesium sulfate; Manganous sulfate 3 parts; 5 parts, zinc sulfate; 3 parts, ferrous sulfate; Boric acid 10 parts; 1 part, copper sulfate.This formula is specially adapted to tomato.
Preparation method is with embodiment 1.
Embodiment 3: environmentally friendly plant source disease-resistant growth-promoting agent, feed composition weight part is as follows:
Taraxacum extracting solution 37 parts; Ginkgo extract 25 parts; Burdock oligosaccharide-copper complex 2 parts; Potassium primary phosphate 8 parts; 2 parts, magnesium sulfate; 5 parts, zinc sulfate; Boric acid 10 parts; 1 part, copper sulfate.This formula is specially adapted to corn.Preparation method is with embodiment 1.
Embodiment 4: environmentally friendly plant source disease-resistant growth-promoting agent, feed composition weight part is as follows:
Herba Taraxaci extract 35 parts; Folium Ginkgo extract 30 parts; Burdock oligosaccharide-copper complex 2 parts; 10 parts, saltpetre; 2 parts, magnesium sulfate; 5 parts, zinc sulfate; 5 parts, ferrous sulfate; Boric acid 10 parts; 1 part, copper sulfate.This formula is specially adapted to
Cucumber.Preparation method is with embodiment 1.
Effect is tested
1, in embodiment 1 wheat private environment friendly plant source disease-resistant growth-promoting agent on the impact of wheat yield and Powdery Mildew
1.1 materials and methods
1.1.1 test materials: wheat breed: 15(Shandong Province, Jinan academy of agricultural sciences crop provided); Bacterial classification: Erysiphe graminis (Erysiphe graminis).Academy of agricultural sciences of Shandong Province Plant Protection Institute provides.
1.1.2 pot experiment:
Pot experiment designs: 4 process are established in this test: (1) contrast (CK): spray clear water; (2) environmentally friendly plant source disease-resistant growth-promoting agent (BADRGP): spray 10gL
-1environmentally friendly plant source disease-resistant growth-promoting agent solution; (3) pathogenic bacteria (E.g.): inoculation Erysiphe graminis; (4) BADRGP+E.g.: spray 10gL
-1environmentally friendly plant source disease-resistant growth-promoting agent solution, inoculated Erysiphe graminis after 3 days.Each processes 6 repetitions, the strain of every basin 10.
Pot-culture method: wheat seed, after 3% hypochlorite disinfectant, rinsing, vernalization, is planted in sterilized soil.Treat that wheat grew to for 2 leaf phases, in process (2) and (4), spray 10gL
-1environmentally friendly plant source disease-resistant growth-promoting agent solution, inoculated the Erysiphe graminis of having bred to process (3) and (4) with shaking off method after 3 days.Then wheat is placed in that humidity is 90%, temperature is in the moisturizing bucket of 25 DEG C, takes out after 16h.Mutually isolated between each process, prevent cross infection, when pending (3) morbidity is more abundant, investigation disease refers to.
Wheat powdery mildew Disease investigation: 0 grade: anosis; 1 grade: lesion area accounts for less than 5% of one-piece blade area; 3 grades: lesion area accounts for 6 ~ 15% of one-piece blade area; 5 grades: lesion area accounts for 16 ~ 25% of one-piece blade area; 7 grades: lesion area accounts for 26 ~ 50% of one-piece blade area; 9 grades: lesion area accounts for more than 50% of one-piece blade area.
Disease index (%)=(Σ (disease index × this grade of diseased plant number) × 100)/(the highest number of the state of an illness × total strain number)
Relative control effect (%)=(contrast disease index-process disease index) × 100/ contrast disease index
1.1.3 field test:
Field experiment designs: 3 process are established in test: (1) contrast (CK): spray clear water; (2) environmentally friendly plant source disease-resistant growth-promoting agent (BADRGP): spray environmentally friendly plant source disease-resistant growth-promoting agent solution; (3) commercially available foliage fertilizer (SSLF): the product Tianda-2116 with disease-resistant function that spray market is bought, 3 repetitions are established in each process, and community adopts the group arrangement of random district.
Field experiment method: test and carry out in high-ranking official village, Longshan Town, Zhangqiu City.Environmentally friendly plant source disease-resistant growth-promoting agent dilutes 500 times when using, and Tianda-2116 dilutes 600 times.Spray.4 ~ 6m is got during results
2wheat, threshing meter produces, according to actual cut area conversion wheat hectare output.
1.2 results and analysis
1.2.1 the impact of environmentally friendly plant source disease-resistant growth-promoting agent induction on potted plant wheat powdery mildew disease index
From table 1, wheat disease index and relative control effect result are found out: do not inoculate the process of pathogenic bacteria not by infection process.Environmentally friendly plant source disease-resistant growth-promoting agent induces the disease index of wheat after 3 days significantly lower than E.g. process, and relative control effect reaches 33.3%.Illustrate that the induction of environmentally friendly plant source disease-resistant growth-promoting agent has certain prophylactic effect to wheat powdery mildew.
Table 1 environmentally friendly plant source disease-resistant growth-promoting agent is on the impact of potted plant wheat powdery mildew disease index
1.2.2 the impact of environmentally friendly plant source disease-resistant growth-promoting agent induction on wheat output
On the impact of wheat output as shown in Figure 2, result shows the induction of environmentally friendly plant source disease-resistant growth-promoting agent, and sprays compared with clear water, and after spraying different foliage fertilizer, wheat yield has and improves to some extent.The wheat yield of environmentally friendly plant source disease-resistant growth-promoting agent process increases by 12.14%, and commercially available foliage fertilizer increases by 9.26%.Explanation sprays environmentally friendly plant source disease-resistant growth-promoting agent can increase wheat yield.
If environmentally friendly plant source disease-resistant growth-promoting agent can merchandized handling smoothly, cost can than experiment, reduction further than pilot scale.Price and commercially available foliage fertilizer similar.Because it also increases production about 2.8% than commercially available foliage fertilizer, if by per mu yield 1000 jin, Wheat price is 1.2 yuan of/jin of calculating, amounts to mu and takes in about 34 yuan more.
1.3 brief summary
Result shows: the induction of environmentally friendly plant source disease-resistant growth-promoting agent can wheat growth-promoting, reduces disease, improves wheat yield.
2, embodiment 2 tomato private environment friendly plant source disease-resistant growth-promoting agent is to the restraining effect of graw mold of tomato
2.1 materials and methods
2.1.1 for examination material
Tomato: middle vegetables No. four (Xinjiang Ji Feng Zhong Ye limited liability company is on sale); Bacterial classification: Botrytis cinerea (Botrytis cinerea), see Dai Fanglan, Chinese fungi aggregation, the 850th ?852 pages, scientific publication, 1979.Academy of agricultural sciences of Shandong Province Plant Protection Institute provides.
2.1.2 pot experiment design and method
4 process are established in this test, are respectively (1) contrast (CK): spray clear water; (2) environmentally friendly plant source disease-resistant growth-promoting agent (BADRGP)
10: spray 10gL
-1environmentally friendly plant source disease-resistant growth-promoting agent solution; (3) (BADRGP)
15: spray 15gL
-1environmentally friendly plant source disease-resistant growth-promoting agent solution; (4) (BADRGP)
20: spray 20gL
-1environmentally friendly plant source disease-resistant growth-promoting agent solution, each process repeats for 5 times.
Tomato seeds, after 3% hypochlorite disinfectant, rinsing, vernalization, is planted in sterilized soil.When tomato grew to for 3 leaf phase, final singling, the strain of every basin 10, during 5 leaf phase, spray environmentally friendly plant source disease-resistant growth-promoting agent solution, inoculate pathogenic bacteria after 3 days, method is streak method, is 1 × 10 by concentration
7the Botrytis cinerea liquid of CFU/ml spreads upon tomato leaf portion, all the other process spray clear water.Then be placed on that humidity is 90%, temperature is in the moisturizing bucket of 20 DEG C, observe incidence, when B.c. process morbidity is abundant, add up disease index.
Disease investigation: 0 grade: without scab; 1 grade: lesion area accounts for whole leaf area less than 5%; 3 grades: lesion area accounts for whole leaf area 6% ~ 10%; 5 grades: lesion area accounts for whole leaf area 11% ~ 20%; 7 grades: lesion area accounts for whole leaf area 21% ~ 40%; 9 grades: lesion area accounts for whole leaf area more than 40%.
Disease index (%)=(Σ (disease index × this grade of diseased plant number) × 100)/(the highest number of the state of an illness × total strain number)
Relative control effect (%)=(contrast disease index-process disease index) × 100/ contrast disease index
2.1.3 booth test design and method
3 process are established in test, are respectively (1) contrast (CK): spray clear water; (2) environmentally friendly plant source disease-resistant growth-promoting agent (BADRGP): spray environmentally friendly plant source disease-resistant growth-promoting agent solution; (3) commercially available foliage fertilizer (SSLF): the foliage fertilizer Tianda-2116 with disease-resistant function that spray market is bought, each process repeats for 3 times, and community arranges by random district group, plot area 21m
2.
Test is carried out in Hou Yang village village, Liu Ge town, Haiyang City, Shandong Province, 3 years ages of greenhouse.Whole community tomato yield is recorded during each results.
2.2 results and analysis
2.2.1 environmentally friendly plant source disease-resistant growth-promoting agent is on the impact of potted tomato gray mold disease index
The environmentally friendly plant source disease-resistant growth-promoting agent of table 2 is on the impact of potted tomato gray mold disease index
Found out by table 2, the tomato disease index of spray different concns environmentally friendly plant source disease-resistant growth-promoting agent process is significantly lower than the process of spray clear water, and effect is it is preferred that (BADRGP)
20process, relative control effect reaches 53.77%, illustrates that the induction of environmentally friendly plant source disease-resistant growth-promoting agent has certain prophylactic effect to graw mold of tomato.
2.2.2 environmentally friendly plant source disease-resistant growth-promoting agent is on the impact of tomato in plastic greenhouse output
Environmentally friendly plant source disease-resistant growth-promoting agent on the impact of tomato in plastic greenhouse output as shown in Figure 3, test-results shows, in greenhouse gardening tomato situation, spray environmentally friendly plant source disease-resistant growth-promoting agent amount of increase in production and reach 22.97%, commercially available foliage fertilizer reaches 19.0%.
After this environmentally friendly plant source disease-resistant growth-promoting agent is used, than commercially available foliage fertilizer output increased nearly 4%, if be 11000 pounds/acre by tomato in plastic greenhouse output in winter, price is 2 yuan of/jin of calculating, mu increases income more than 880 yuan, and therefore environmentally friendly plant source disease-resistant growth-promoting agent benefit is better, has a extensive future.
2.3 brief summary
Result shows, environmentally friendly plant source disease-resistant growth-promoting agent induction can improve the ability of tomato botrytis resistant, and sickness rate is reduced, and output increases, and benefit improves.
Claims (6)
1. a preparation method for environmentally friendly plant source disease-resistant growth-promoting agent, feed composition weight part is as follows:
Herba Taraxaci extract 30 ~ 50 parts; Folium Ginkgo extract 20 ~ 40 parts; Burdock oligosaccharide-copper complex 1 ~ 2 part; Potassium primary phosphate or 5 ~ 10 parts, saltpetre; 0 ~ 2 part, magnesium sulfate; Manganous sulfate 0 ~ 5 part; 2 ~ 5 parts, zinc sulfate; 0 ~ 10 part, ferrous sulfate; Boric acid 1 ~ 10 part; 0 ~ 1 part, copper sulfate; Chelator acid citrate consumption is 5 ~ 15wt% of medium trace element consumption;
Described Herba Taraxaci extract Content of Chlorogenic Acid >=2%, flavonoid content >=1.5%; Alkaloid >=5.0% in described Folium Ginkgo extract, flavonoid content >=1.0%;
Described Herba Taraxaci extract is that lixiviate solid-to-liquid ratio is taraxacum dry powder by the filtrate of hot water extraction after the leaf of taraxacum and/or stem drying and crushing: water=1:25 ~ 30 weight ratio;
Described Semen Ginkgo extrac is by the filtrate of Ginkgo Leaf dry powder hot water extraction, and lixiviate solid-to-liquid ratio is Ginkgo Leaf dry powder: water=1:35 ~ 40 weight ratio;
Described burdock oligosaccharide-copper complex is formed by burdock oligosaccharide and copper sulfate complexing, prepare according to the following steps: burdock oligosaccharide is dissolved in distilled water, copper-bath is joined in burdock oligosaccharide solution, adjust pH5.5 ~ 6.5, in 50 DEG C of stirred in water bath backflows 1 ~ 2 hour, concentrated, add acetone and separate out precipitation, after filtration, washing, drying, recrystallization, vacuum-drying, obtain burdock oligosaccharide-copper complex;
Step is as follows:
(1) preparation of Herba Taraxaci extract, Folium Ginkgo extract
By hot water extraction after the leaf of taraxacum and/or stem drying and crushing, temperature 50 ~ 70 DEG C, lixiviate solid-to-liquid ratio is taraxacum dry powder: water=1:25 ~ 30 weight ratio, extraction time is 1 ~ 3 hour, vat liquor filters, chlorogenic acid content >=2% of gained stoste, flavonoid content >=1.5%; I.e. Herba Taraxaci extract;
By Ginkgo Leaf dry powder hot water extraction, temperature 50 ~ 70 DEG C, lixiviate solid-to-liquid ratio is Ginkgo Leaf dry powder: water=1:35 ~ 40 weight ratio, and extraction time is 1 ~ 3 hour, and vat liquor filters, alkaloid >=5.0% of gained stoste, flavonoid content >=1.0%; I.e. Folium Ginkgo extract;
Above-mentioned leaching process completes in plant concentration extraction device;
(2) by proportioning, Herba Taraxaci extract obtained for step (1), Folium Ginkgo extract are added in reactor, thin up 20-30 times volume;
(3) by proportioning, in reactor, add medium trace element magnesium sulfate, manganous sulfate, zinc sulfate, ferrous sulfate, boric acid, copper sulfate, stirring and dissolving, then add chelator acid citrate, be heated to 60 ~ 80 DEG C, adjust pH to 4.5 ~ 6.5, react 0.5 ~ 1 hour;
(4) after completion of the reaction, be cooled to 25 ~ 30 DEG C, in reactor, add burdock oligosaccharide-copper complex, potassium primary phosphate or saltpetre, dissolve, stirring is mixed evenly, and adjusts pH to 5.5 ~ 6.5; Employing vacuum filter filters, and fully stirs further, make mixed solution even in liquid sand mill; Obtain.
2. the preparation method of environmentally friendly plant source disease-resistant growth-promoting agent as claimed in claim 1, it is characterized in that, feed composition weight part is as follows:
Taraxacum extracting solution 34 parts; Ginkgo extract 40 parts; Burdock oligosaccharide-copper complex 2 parts; 8 parts, saltpetre; 2 parts, magnesium sulfate; 3 parts, zinc sulfate; Boric acid 10 parts; 1 part, copper sulfate;
This formula is the special disease-resistant growth-promoting agent of wheat.
3. the preparation method of environmentally friendly plant source disease-resistant growth-promoting agent as claimed in claim 1, it is characterized in that, feed composition weight part is as follows:
Taraxacum extracting solution 30 parts; Ginkgo extract 35 parts; Burdock oligosaccharide-copper complex 2 parts; Potassium primary phosphate 10 parts; 2 parts, magnesium sulfate; Manganous sulfate 3 parts; 5 parts, zinc sulfate; 3 parts, ferrous sulfate; Boric acid 10 parts; 1 part, copper sulfate; This formula is the special disease-resistant growth-promoting agent of tomato.
4. the preparation method of environmentally friendly plant source disease-resistant growth-promoting agent as claimed in claim 1, it is characterized in that, feed composition weight part is as follows:
Taraxacum extracting solution 37 parts; Ginkgo extract 25 parts; Burdock oligosaccharide-copper complex 2 parts; Potassium primary phosphate 8 parts; 2 parts, magnesium sulfate; 5 parts, zinc sulfate; Boric acid 10 parts; 1 part, copper sulfate; This formula is the special disease-resistant growth-promoting agent of corn.
5. the preparation method of environmentally friendly plant source disease-resistant growth-promoting agent as claimed in claim 1, it is characterized in that, feed composition weight part is as follows:
Herba Taraxaci extract 35 parts; Folium Ginkgo extract 30 parts; Burdock oligosaccharide-copper complex 2 parts; 10 parts, saltpetre; 2 parts, magnesium sulfate; 5 parts, zinc sulfate; 5 parts, ferrous sulfate; Boric acid 10 parts; 1 part, copper sulfate; This formula is the special disease-resistant growth-promoting agent of cucumber.
6. the preparation method of environmentally friendly plant source disease-resistant growth-promoting agent as claimed in claim 1, it is characterized in that also adding Surfactant OP-10 in the environmentally friendly plant source disease-resistant growth-promoting agent of gained, mix, canned, the consumption of tensio-active agent is 0.1 ~ 0.8 wt % of feed composition total amount.
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| CN104130065A (en) * | 2014-07-30 | 2014-11-05 | 金美善 | Plate growth promoter and preparation method thereof |
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| CN109020744A (en) * | 2018-10-30 | 2018-12-18 | 潘云龙 | Langenaria siceria plants special medicated fertilizer and preparation method thereof |
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