CN113354491A - Biocontrol and plant vigor complexing agent and preparation method thereof - Google Patents
Biocontrol and plant vigor complexing agent and preparation method thereof Download PDFInfo
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- CN113354491A CN113354491A CN202110822862.2A CN202110822862A CN113354491A CN 113354491 A CN113354491 A CN 113354491A CN 202110822862 A CN202110822862 A CN 202110822862A CN 113354491 A CN113354491 A CN 113354491A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/30—Layered or coated, e.g. dust-preventing coatings
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Soil Sciences (AREA)
- Plant Pathology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a biocontrol and plant vigor complexing agent and a preparation method thereof. The compound agent has reasonable design, has stronger biocontrol capability, can improve the plant immunity, enhance the disease resistance of plants, increase roots and strengthen seedlings, thereby promoting the photosynthesis of crops, leading the plants to absorb nutrient elements required by growth from soil, promoting the growth and maturing in advance, and having the effects of improving soil, protecting environment and the like.
Description
Technical Field
The invention relates to the technical field of bioengineering application, in particular to a biocontrol and plant vigor complexing agent and a preparation method thereof.
Background
Population, food and environment have become the current outstanding problem, and by 2040 years, food production must be increased 3 times on the current basis to meet human development needs, even if pesticides are used, 30% of crops are lost every year, otherwise 70% is lost. However, the use of chemical pesticides causes side effects such as resistance to the plant, direct harm to non-target organisms, environmental pollution, neglect of natural regulation, etc., which make pest control more complicated and difficult, and there is a strong need for effective and feasible methods to solve these problems. The world society of environmental and developmental activities as early as 1992 has required "control of the sale and use of chemical pesticides on a global scale", and it has become a global consensus that chemical pesticides cannot be relied upon excessively. When Moffat faces prohibition of using chemical pesticides to the environment, Moffat points out that the research of novel biocontrol agents by using genetic and genetic engineering techniques is the fundamental way to solve the problems of food and environment.
The plant biocontrol agent is a general name for preparing metabolites for controlling plant diseases and insect pests, weeds and regulating plant growth by utilizing various bioactive components generated or expressed by organisms and genes thereof. The plant biocontrol preparation has the following advantages: (1) the specificity for preventing and controlling the plant diseases and insect pests is strong; (2) is safe and nontoxic to human and livestock, does not pollute the environment and has no residue; (3) the killing specificity to germs and pests is strong, natural enemies and beneficial organisms are not damaged, and ecological natural balance can be kept; (4) the production raw materials and the effective components belong to natural products, and the natural products can return to the nature, so that the sustainable development is ensured; (5) the microorganism can be modified by biotechnology and genetic engineering method, and has various development and utilization ways, and pests and pathogenic bacteria are difficult to generate drug resistance.
In the prior art, a biocontrol agent and a plant nutrition activator are not compounded.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a biocontrol and plant activity complexing agent which comprises 560mg of sodium benzoate 530-, 20-35mg of ferric sulfate, 20000-40000mg of sucrose, 80-150mg of inositol, 0.01-1mg of IVB nicotinic acid, 0.01-1mg of pyridoxine hydrochloride, 0.01-1mg of thiamine hydrochloride and 0.5-5mg of glycine.
As a preferred technical solution of the present invention, the plant extract includes green tea polyphenol, soy isoflavone, bamboo leaf extract, grape seed extract, rosemary extract, litchi essential oil, cinnamaldehyde, ginkgo leaf extract and ginger extract.
A preparation method of a biocontrol and plant vigor complexing agent comprises the following specific steps:
a. preparing a solid culture medium for culturing a biocontrol agent: the culture medium contains yeast extract 1gL, potato 200gL, glucose 15gL, sodium chloride 3gL and agar 15.0-20gL, the pH value is 6.6-7.2, the culture medium is sterilized for 20 minutes at 122 ℃, taken out after the sterilization is finished, cooled to 45-50 ℃, and then subjected to aseptic operation to pour the plate, and the plate is placed for standby after solidification;
b. preparing a plant vigor culture: 2.6-5.3 parts of nano silicon dioxide, 0.18-0.36 part of sodium stearate, 0.24-0.54 part of polyethylene glycol, 0.13-0.28 part of bisphenol A polyoxypropylene PO-9 ether, 0.12-0.29 part of bisphenol A polyoxyethylene EO-7 ether, 0.24-0.56 part of span and 90-110 parts of water, adding sodium hydroxide to adjust the pH to 12-13, and uniformly mixing the phytoalexin culture with a plate culture medium after the preparation is finished to obtain a biocontrol and phytoalexin culture;
c, preparing a biocontrol and plant vigor complexing agent: mixing the biocontrol and plant vigor culture with sodium benzoate, potassium sorbate, chitosan, phytic acid, lactic acid bacteria, bacteriocin, antimicrobial peptide, protamine, lysozyme, plant extracts, ammonium nitrate, potassium nitrate, calcium chloride, monopotassium phosphate, potassium iodide, boric acid, magnesium sulfate, zinc sulfate, sodium molybdate, copper sulfate, cobalt chloride, ferric sulfate, sucrose, inositol, IVB nicotinic acid, pyridoxine hydrochloride, thiamine hydrochloride and glycine according to the weight ratio to obtain the biocontrol and plant vigor complexing agent.
As a preferred technical scheme of the invention, the plant vigor culture is cultured for 8-11 days at the temperature of 23-26 ℃ after being inoculated in the step b.
As a preferable technical scheme of the invention, the particle diameter of the nano silicon dioxide in the step b is 25-50 nanometers
Compared with the prior art, the invention has the following beneficial effects:
the compound agent has reasonable design, has stronger biocontrol capability, can improve the plant immunity, enhance the disease resistance of plants, increase roots and strengthen seedlings, thereby promoting the photosynthesis of crops, leading the plants to absorb nutrient elements required by growth from soil, promoting the growth and maturing in advance, and having the effects of improving soil, protecting environment and the like.
Detailed Description
The following description of the preferred embodiments of the present invention is provided for the purpose of illustration and description, and is in no way intended to limit the invention.
Example 1
The invention provides a biocontrol and plant vigor complexing agent, which comprises 560mg of sodium benzoate 530-, Sucrose 20000-40000mg, inositol 80-150mg, IVB nicotinic acid 0.01-1mg, pyridoxine hydrochloride 0.01-1mg, thiamine hydrochloride 0.01-1mg and glycine 0.5-5 mg.
The invention provides a preparation method of a biocontrol and plant vigor complexing agent, which comprises the following specific steps:
a. preparing a solid culture medium for culturing a biocontrol agent: the culture medium contains yeast extract 1gL, potato 200gL, glucose 15gL, sodium chloride 3gL and agar 15.0-20gL, the pH value is 6.6-7.2, the culture medium is sterilized for 20 minutes at 122 ℃, taken out after the sterilization is finished, cooled to 45-50 ℃, and then subjected to aseptic operation to pour the plate, and the plate is placed for standby after solidification;
b. preparing a plant vigor culture: 2.6-5.3 parts of nano silicon dioxide, 0.18-0.36 part of sodium stearate, 0.24-0.54 part of polyethylene glycol, 0.13-0.28 part of bisphenol A polyoxypropylene PO-9 ether, 0.12-0.29 part of bisphenol A polyoxyethylene EO-7 ether, 0.24-0.56 part of span and 90-110 parts of water, adding sodium hydroxide to adjust the pH to 12-13, and uniformly mixing the phytoalexin culture with a plate culture medium after the preparation is finished to obtain a biocontrol and phytoalexin culture;
c, preparing a biocontrol and plant vigor complexing agent: mixing the biocontrol and plant vigor culture with sodium benzoate, potassium sorbate, chitosan, phytic acid, lactic acid bacteria, bacteriocin, antimicrobial peptide, protamine, lysozyme, plant extracts, ammonium nitrate, potassium nitrate, calcium chloride, monopotassium phosphate, potassium iodide, boric acid, magnesium sulfate, zinc sulfate, sodium molybdate, copper sulfate, cobalt chloride, ferric sulfate, sucrose, inositol, IVB nicotinic acid, pyridoxine hydrochloride, thiamine hydrochloride and glycine according to the weight ratio to obtain the biocontrol and plant vigor complexing agent.
Specifically, a, preparing a solid culture medium for culturing a biocontrol agent: cutting 200g of potatoes into strips, boiling in 800ml of water in a beaker for 20min, filtering with gauze to remove residues, adding 1.0g of yeast extract, 15g of glucose, 3g of sodium chloride and 15.0-20g of agar into the filtrate, supplementing water to 1000ml, adjusting the pH to 6.6-7.2, subpackaging the obtained mixture into 300ml triangular flasks according to 150ml of culture medium in each bottle, carrying out high-pressure steam sterilization at 122 ℃ for 20min after the packing of the bottle mouths, taking out the obtained product after the sterilization is finished, carrying out aseptic operation when the product is cooled to 45 ℃, pouring the obtained product into a flat plate, and standing for later use after the flat plate is solidified;
b. preparing a plant vigor culture: 2.6 parts of nano silicon dioxide, 0.18 part of sodium stearate, 0.24 part of polyethylene glycol, 0.13 part of bisphenol A polyoxypropylene PO-9 ether, 0.12 part of bisphenol A polyoxyethylene EO-7 ether, 0.24 part of span and 90 parts of water, adding sodium hydroxide to adjust the pH value to 12-13, uniformly mixing the phytovitamine culture with a plate culture medium after the preparation is finished, and detecting that the concentration of the phytovitamine can reach 5.8-110gl for biocontrol and phytovitamine;
c. preparing a biocontrol and plant vigor complexing agent: 20g of biocontrol and plant activity culture, 530mg of sodium benzoate, 480mg of potassium sorbate, 10mg of chitosan, 5mg of phytic acid, 6mg of lactic acid bacteria, 10g of bacteriocin, 3mg of antimicrobial peptide, 4mg of protamine, 1.5mg of lysozyme, 20mg of plant extract, 1200mg of ammonium nitrate, 1500mg of potassium nitrate, 250mg of calcium chloride, 50mg of monopotassium phosphate, 0.1mg of potassium iodide, 2mg of boric acid, 15mg of magnesium sulfate, 1mg of zinc sulfate, 0.1mg of sodium molybdate, 0.001mg of copper sulfate, 0.001mg of cobalt chloride, 20mg of ferric sulfate, 20000mg of sucrose, 80mg of inositol, 0.01mg of IVB nicotinic acid, 0.01mg of pyridoxine hydrochloride, 0.01mg of thiamine hydrochloride and 0.5mg of glycine are taken and mixed uniformly to prepare the novel biocontrol and plant activity composite agent.
The obtained novel biocontrol and plant vigor complexing agent is dissolved in 1000ml of water, when in use, 1/2 is diluted, the mixture is sprayed to the contact part of the plant root and the soil by a spray can or a sprayer, and each plant is about 5g, and through analysis, the novel biocontrol and plant vigor complexing agent disclosed by the invention can be used for receiving more than 1000 yuan per mu compared with the common pesticide.
Example 2
a. Preparing a plant vigor culture: 3.2 parts of nano silicon dioxide, 0.23 part of sodium stearate, 0.36 part of polyethylene glycol, 0.15 part of bisphenol A polyoxypropylene PO-9 ether, 0.22 part of bisphenol A polyoxyethylene EO-7 ether, 0.45 part of span and 95 parts of water, adding sodium hydroxide to adjust the pH value to 12-13, uniformly mixing a plant vigor culture with a plate culture medium after the preparation is finished to obtain a biocontrol and plant vigor culture, inoculating 18g of the plant vigor culture to the center of the plate obtained in example 1, culturing at 22 ℃ for 10 days, uniformly mixing the plant vigor culture with the plate after the culture is finished, and detecting that the concentration of the plant vigor culture can reach 6.9-80gl to obtain the biocontrol and plant vigor culture;
b. preparing a biocontrol and plant vigor complexing agent: 20g of biocontrol and plant activity culture, 560mg of sodium benzoate, 495mg of potassium sorbate, 15mg of chitosan, 6mg of phytic acid, 9mg of lactic acid bacteria, 12mg of bacteriocin, 3mg of antimicrobial peptide, 5mg of protamine, 1.7mg of lysozyme, 25mg of plant extract, 1400mg of ammonium nitrate, 1600mg of potassium nitrate, 350mg of calcium chloride, 130mg of monopotassium phosphate, 0.5mg of potassium iodide, 6mg of boric acid, 18mg of magnesium sulfate, 3mg of zinc sulfate, 0.3mg of sodium molybdate, 0.005mg of copper sulfate, 0.05mg of cobalt chloride, 27mg of ferric sulfate, 30000mg of sucrose, 105mg of inositol, 0.08mg of IVB nicotinic acid, 0.06mg of pyridoxine hydrochloride, 0.07mg of thiamine hydrochloride and 3mg of glycine are taken and mixed uniformly to prepare the novel biocontrol and plant activity composite preparation.
The novel biocontrol and plant vigor complexing agent can also achieve the same technical effect as the embodiment 1.
Example 3
a. Preparing a plant vigor culture: 5.3 parts of nano silicon dioxide, 0.36 part of sodium stearate, 0.54 part of polyethylene glycol, 0.28 part of bisphenol A polyoxypropylene PO-9 ether, 0.29 part of bisphenol A polyoxyethylene EO-7 ether, 0.56 part of span and 110 parts of water, adding sodium hydroxide to adjust the pH value to 12-13, and uniformly mixing a phytoalexin culture and a plate culture medium after the preparation is finished to obtain a biocontrol and phytoalexin culture for biocontrol and phytoalexin culture;
b. preparing a biocontrol and plant vigor complexing agent: 20g of biocontrol and plant activity culture, 550mg of sodium benzoate, 500mg of potassium sorbate, 12mg of chitosan, 6mg of phytic acid, 8mg of lactic acid bacteria, 12mg of bacteriocin, 4mg of antimicrobial peptide, 5mg of protamine, 2mg of lysozyme, 26mg of plant extract, 1300mg of ammonium nitrate, 1600mg of potassium nitrate, 300mg of calcium chloride, 250mg of monopotassium phosphate, 1.2mg of potassium iodide, 8mg of boric acid, 30mg of magnesium sulfate, 5mg of zinc sulfate, 0.5mg of sodium molybdate, 0.05mg of copper sulfate, 0.05mg of cobalt chloride, 35mg of ferric sulfate, 40000mg of sucrose, 150mg of inositol, 1mg of IVB nicotinic acid, 1mg of pyridoxine hydrochloride, 1mg of thiamine hydrochloride and 5mg of glycine are taken, and the substances are mixed uniformly to prepare the novel biocontrol and plant activity complexing agent.
The novel biocontrol and plant vigor complexing agent can also achieve the same technical effect as the embodiment 1.
Example 4
a. Preparing a plant vigor culture: 2.6-5.3 parts of nano silicon dioxide, 0.27 part of sodium stearate, 0.32 part of polyethylene glycol, 0.17 part of bisphenol A polyoxypropylene PO-9 ether, 0.29 part of bisphenol A polyoxyethylene EO-7 ether, 0.36 part of span and 105 parts of water, adding sodium hydroxide to adjust the pH value to 12-13, uniformly mixing a phyto-activin culture with a plate culture medium after the preparation is finished to obtain a biocontrol and phyto-activin culture, inoculating 16g of the phyto-activin culture into the center of the plate obtained in example 1, culturing for 10 days at 22 ℃, uniformly mixing the phyto-activin culture with the plate after the culture is finished, and detecting that the concentration of the phyto-activin can reach 6.9-70gl and the biocontrol and phyto-activin culture is obtained;
b. preparing a biocontrol and plant vigor complexing agent: 30g of biocontrol and plant activity culture, 550mg of sodium benzoate, 500mg of potassium sorbate, 14mg of chitosan, 6mg of phytic acid, 8mg of lactic acid bacteria, 14mg of bacteriocin, 5mg of antimicrobial peptide, 6mg of protamine, 2.0mg of lysozyme, 35mg of plant extract, 1600mg of ammonium nitrate, 1700mg of potassium nitrate, 4500mg of calcium chloride, 180mg of monopotassium phosphate, 0.8mg of potassium iodide, 6mg of boric acid, 18mg of magnesium sulfate, 4mg of zinc sulfate, 0.3mg of sodium molybdate, 0.03mg of copper sulfate, 0.009mg of cobalt chloride, 29mg of ferric sulfate, 35000mg of sucrose, 109mg of inositol, 0.09mg of IVB nicotinic acid, 0.2mg of pyridoxine hydrochloride, 0.6mg of thiamine hydrochloride and 3.6mg of glycine are taken, and the above substances are mixed uniformly to prepare the novel biocontrol and plant activity compound.
The novel biocontrol and plant vigor complexing agent can also achieve the same technical effect as the embodiment 1.
The compound agent has reasonable design, has stronger biocontrol capability, can improve the plant immunity, enhance the disease resistance of plants, increase roots and strengthen seedlings, thereby promoting the photosynthesis of crops, leading the plants to absorb nutrient elements required by growth from soil, promoting the growth and maturing in advance, and having the effects of improving soil, protecting environment and the like.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (5)
1. A biocontrol and plant vigor complexing agent is characterized in that the biocontrol and plant vigor complexing agent comprises 560mg of sodium benzoate 530-, Sucrose 20000-40000mg, inositol 80-150mg, IVB nicotinic acid 0.01-1mg, pyridoxine hydrochloride 0.01-1mg, thiamine hydrochloride 0.01-1mg and glycine 0.5-5 mg.
2. The biocontrol and plant vigor complexing agent as defined in claim 1, wherein the plant extract comprises green tea polyphenols, soy isoflavones, bamboo leaf extract, grape seed extract, rosemary extract, litchi chinensis seed oil, cinnamaldehyde, ginkgo biloba extract and ginger extract.
3. A preparation method of a biocontrol and plant vigor complexing agent is characterized by comprising the following specific steps:
a. preparing a solid culture medium for culturing a biocontrol agent: the culture medium contains yeast extract 1gL, potato 200gL, glucose 15gL, sodium chloride 3gL and agar 15.0-20gL, the pH value is 6.6-7.2, the culture medium is sterilized for 20 minutes at 122 ℃, taken out after the sterilization is finished, cooled to 45-50 ℃, and then subjected to aseptic operation to pour the plate, and the plate is placed for standby after solidification;
b. preparing a plant vigor culture: 2.6-5.3 parts of nano silicon dioxide, 0.18-0.36 part of sodium stearate, 0.24-0.54 part of polyethylene glycol, 0.13-0.28 part of bisphenol A polyoxypropylene PO-9 ether, 0.12-0.29 part of bisphenol A polyoxyethylene EO-7 ether, 0.24-0.56 part of span and 90-110 parts of water, adding sodium hydroxide to adjust the pH to 12-13, and uniformly mixing the phytoalexin culture with a plate culture medium after the preparation is finished to obtain a biocontrol and phytoalexin culture;
c, preparing a biocontrol and plant vigor complexing agent: mixing the biocontrol and plant vigor culture with sodium benzoate, potassium sorbate, chitosan, phytic acid, lactic acid bacteria, bacteriocin, antimicrobial peptide, protamine, lysozyme, plant extracts, ammonium nitrate, potassium nitrate, calcium chloride, monopotassium phosphate, potassium iodide, boric acid, magnesium sulfate, zinc sulfate, sodium molybdate, copper sulfate, cobalt chloride, ferric sulfate, sucrose, inositol, IVB nicotinic acid, pyridoxine hydrochloride, thiamine hydrochloride and glycine according to the weight ratio to obtain the biocontrol and plant vigor complexing agent.
4. The preparation method of the biocontrol and plant vigor complexing agent is characterized in that after the plant vigor culture is inoculated in the step b, the mixture is cultured for 8 to 11 days at the temperature of between 23 and 26 ℃.
5. The preparation method of the biocontrol and plant vigor complexing agent is characterized in that the particle size of the nano silicon dioxide in the step b is 25-50 nanometers.
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