CN103193547A - Environment-friendly plant source disease-resistant growth promoting agent and preparation method thereof - Google Patents
Environment-friendly plant source disease-resistant growth promoting agent and preparation method thereof Download PDFInfo
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- CN103193547A CN103193547A CN2013101533649A CN201310153364A CN103193547A CN 103193547 A CN103193547 A CN 103193547A CN 2013101533649 A CN2013101533649 A CN 2013101533649A CN 201310153364 A CN201310153364 A CN 201310153364A CN 103193547 A CN103193547 A CN 103193547A
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Abstract
The invention relates to an environment-friendly plant source disease-resistant growth promoting agent and a preparation method of the environment-friendly plant source disease-resistant growth promoting agent. According to the plant source disease-resistant growth promoting agent, a dandelion extract, a ginkgo biloba extract and a burdock oligosaccharide-copper complex are used as main components and are mixed with monopotassium phosphate or potassium nitrate, magnesium sulfate, zinc sulfate, ferrous sulfate, boric acid and copper sulfate serving as large nutrient elements, medium nutrient elements and micronutrient elements; and the plant source disease-resistant growth promoting agent is prepared by compounding, chelating and fully stirring. Combining nutrition and disease resistance into a whole, the plant source disease-resistant growth promoting agent is wide in spectrum resistance; and by improving the immunity of a crop, the health of the plant is increased, the disease resistance of the plant is increased, pesticide application amount is reduced, the yield and the quality of the crop are improved, the performance is stable and the environment is friendly.
Description
Technical field
The present invention relates to a kind of environmentally friendly plant source disease-resistant growth-promoting agent and preparation method thereof, belong to the short technical field of giving birth to of plant stress-resistance.
Background technology
The residual meeting of the chemical pesticide that produces in the agriculture production causes that toxic substance exceeds standard in water body, soil and the agricultural-food, seriously jeopardizes human beings'health and value of farm production.Therefore press for development high-quality, non-harmful agricultural-food.And the safeguards of production high-quality, non-polluted farm product is healthy cultivation.Bio-control method is the primary selection of healthy cultivation, comprises that mainly microorganism builds biological control material, plant-sourced class biological control material and plant inducing anti-disease etc.For example CN1946297A provides a kind of plant disease controlling agent or control material, and described control agent or control material comprise bacillus (Bacillus) bacterium that can suppress plant pathogenetic bacteria, fungi or virus infection or propagation.CN101433221A discloses a kind of traditional Chinese medicine bactericide of preventing and treating konjak soft rot, belongs to control of plant disease and field of biological pesticide.Its main ingredient and weight percent are: Bulbus Allii extract 12%～36%, and Rhizoma Coptidis extract 3%～12%, Folium Lonicerae extract 3%～10%, agricultural chemicals wettable powder agent aid 3%～5%, surplus is the agricultural chemicals filling carrier.Above-mentioned each component is re-dubbed konjak soft rot pathogenic bacterium carrot soft rot erwinia is had apparent the traditional Chinese medicine bactericide wettable powder of sterilization effect, be used for the konjak soft rot control.This traditional Chinese medicine bactericide wettable powder environmental protection, its specificity of evidence is strong, and the control konjak soft rot is better than chemical agent, and relative control effect is higher than 65%, is applied to can improve konjaku output in the konjaku production.
It is disease-resistant relative lasting, stable and often have a systematicness to a large number of experiments show that botanical pesticide and plant inducer adpedance characteristic of disease have; Disease-resistant spectrum is wide, free of contamination characteristics.
Though botanical pesticide and plant inducer adpedance characteristic of disease have obtained the extensive recognition and acceptance of society, exist the single problem of formulation in current development research.Therefore be difficult to adapt to the demand of numerous control of plant disease, need further develop and integrate nutrition, disease-resistant environmentally friendly plant source disease-resistant growth-promoting agent.
Summary of the invention
At the deficiencies in the prior art, the invention provides a kind of environmentally friendly plant source disease-resistant growth-promoting agent and preparation method thereof.
Technical scheme of the present invention is as follows:
Environmentally friendly plant source disease-resistant growth-promoting agent, the feed composition weight part is as follows:
30～50 parts of Herba Taraxaci extracts; 20～40 parts of Folium Ginkgo extracts; 1～2 part of burdock oligosaccharide-copper complex; 5～10 parts in potassium primary phosphate or saltpetre; 0～2 part in sal epsom; 0～5 part of manganous sulfate; 2～5 parts in zinc sulfate; 0～10 part in ferrous sulfate; 1～10 part of boric acid; 0～1 part in copper sulfate;
Chlorogenic acid content 〉=2% in the described taraxacum stem and leaf extract, flavonoid content 〉=1.5%; Alkaloid in the described Folium Ginkgo extract 〉=5.0%, flavonoid content 〉=1.0%;
Described Herba Taraxaci extract is that the lixiviate solid-to-liquid ratio is taraxacum dry powder: water=1:25～30 weight ratios with the filtrate of the leaf of taraxacum and/or the dry pulverizing after heat of stem flooding;
Described Semen Ginkgo extrac is the filtrate by the lixiviate of Ginkgo Leaf dry powder hot water, and the lixiviate solid-to-liquid ratio is Ginkgo Leaf dry powder: water=1:35～40 weight ratios.
Described burdock oligosaccharide-copper complex is to be formed by burdock oligosaccharide and copper sulfate complexing.
Above Herba Taraxaci extract and Semen Ginkgo extrac, not special all getting final product by the prior art extraction process by water of limiting.
According to the present invention, burdock oligosaccharide-copper complex formazan preparation gets final product by existing burdock oligosaccharide complex compound preparation technology.The present invention preferably provides following method:
Described burdock oligosaccharide-copper complex prepares according to the following steps: burdock oligosaccharide is dissolved in the distilled water, copper-bath is joined in the burdock oligosaccharide solution, transfer pH5.5～6.5, refluxed 1～2 hour in 50 ℃ of stirred in water bath, concentrate, add acetone and separate out precipitation, after filtration, washing, drying, recrystallization, vacuum-drying, obtain burdock oligosaccharide-copper complex.
In feed composition of the present invention, potassium primary phosphate or saltpetre are the source of macroelement K, and among Mg, Zn, Mn, Fe, the Cu etc., trace element all selects its vitriol form for use, B selects boric acid for use; This macroelement and in, trace element can be plant growth that nutrition is provided.
Preferred according to the present invention, environmentally friendly plant source disease-resistant growth-promoting agent, the feed composition weight part is as follows:
34 parts of taraxacum extracting solutions; 40 parts of ginkgo extracts; 2 parts of burdock oligosaccharide-copper complexs; 8 parts in saltpetre; 2 parts in sal epsom; 3 parts in zinc sulfate; 10 parts of boric acid; 1 part in copper sulfate.This prescription is the special-purpose disease-resistant growth-promoting agent of wheat.
Preferred according to the present invention, environmentally friendly plant source disease-resistant growth-promoting agent, the feed composition weight part is as follows:
30 parts of taraxacum extracting solutions; 35 parts of ginkgo extracts; 2 parts of burdock oligosaccharide-copper complexs; 10 parts of potassium primary phosphates; 2 parts in sal epsom; 3 parts of manganous sulfates; 5 parts in zinc sulfate; 3 parts in ferrous sulfate; 10 parts of boric acid; 1 part in copper sulfate.This prescription is the special-purpose disease-resistant growth-promoting agent of tomato.
Preferred according to the present invention, environmentally friendly plant source disease-resistant growth-promoting agent, the feed composition weight part is as follows:
37 parts of taraxacum extracting solutions; 25 parts of ginkgo extracts; 2 parts of burdock oligosaccharide-copper complexs; 8 parts of potassium primary phosphates; 2 parts in sal epsom; 5 parts in zinc sulfate; 10 parts of boric acid; 1 part in copper sulfate.This prescription is the special-purpose disease-resistant growth-promoting agent of corn.
Preferred according to the present invention, environmentally friendly plant source disease-resistant growth-promoting agent, the feed composition weight part is as follows:
35 parts of Herba Taraxaci extracts; 30 parts of Folium Ginkgo extracts; 2 parts of burdock oligosaccharide-copper complexs; 10 parts in saltpetre; 2 parts in sal epsom; 5 parts in zinc sulfate; 5 parts in ferrous sulfate; 10 parts of boric acid; 1 part in copper sulfate.This prescription is the special-purpose disease-resistant growth-promoting agent of cucumber.
According to the present invention, the preparation method of environmentally friendly plant source disease-resistant growth-promoting agent, step is as follows:
(1) preparation of Herba Taraxaci extract, Folium Ginkgo extract
With leaf and/or the dry pulverizing after heat of the stem flooding of taraxacum, 50～70 ℃ of temperature, lixiviate solid-to-liquid ratio are taraxacum dry powder: water=1:25～30 weight ratios, extraction time is 1～3 hour, vat liquor filters, the chlorogenic acid content of gained stoste 〉=2%, flavonoid content 〉=1.5%; It is Herba Taraxaci extract;
With the lixiviate of Ginkgo Leaf dry powder hot water, 50～70 ℃ of temperature, lixiviate solid-to-liquid ratio are Ginkgo Leaf dry powder: water=1:35～40 weight ratios, and extraction time is 1～3 hour, and vat liquor filters, the alkaloid of gained stoste 〉=5.0%, flavonoid content 〉=1.0%; It is Folium Ginkgo extract;
(2) by proportioning, Herba Taraxaci extract, Folium Ginkgo extract that step (1) is made add in the reactor thin up 20-30 times volume;
(3) by proportioning, micro-sal epsom, manganous sulfate, zinc sulfate, ferrous sulfate, boric acid, copper sulfate in adding in the reactor, stirring and dissolving adds sequestrant citric acid or ethylenediamine tetraacetic acid (EDTA) again, be heated to 60～80 ℃, adjust pH to 4.5～6.5, reacted 0.5～1 hour;
(4) after reaction finishes, be cooled to 25～30 ℃, in reactor, add burdock oligosaccharide-copper complex, potassium primary phosphate or saltpetre, dissolving, stirring is evenly mixed, transfers pH to 5.5～6.5; Filter, further fully stir, make mixed solution even.Namely get environmentally friendly plant source disease-resistant growth-promoting agent.
Preferably, as needs prolonged preservation and long-distance transport, can in above-mentioned environmentally friendly plant source disease-resistant growth-promoting agent, add tensio-active agent again, mix, canned, get environmentally friendly plant source disease-resistant growth-promoting agent product, be used for direct marketing.The consumption of tensio-active agent is 0.1～0.8wt% of feed composition total amount.
The method according to this invention, preferred, the leaching process of step (1) is finished in plant concentration extraction device.
The method according to this invention, preferred, the sequestrant citric acid consumption of step (3) is 5～15wt% of middle micro-consumption.
The method according to this invention, preferred, vacuum filter is adopted in the filtration of step (4).
The method according to this invention, preferred, further fully being stirred in the liquid sand mill of step (4) carried out.
The method according to this invention, adding tensio-active agent in the growth-promoting agent product is in order to guarantee the stable and uniform of product system, preferably, described tensio-active agent is selected from alkylphenol polyoxyethylene OP series or sapn series, as OP-9,10,12, one of 20, Span-20,40,60, one of 80, further preferred described tensio-active agent is OP-10.
Compared with the prior art the present invention has the following advantages:
1, the present invention is solvent with Folium Taraxacum and stem extracting solution, ginkgo biloba succi, the required specific trace element of dissolving plant, and the environmentally friendly plant source disease-resistant growth-promoting agent of making has taken full advantage of the disease-resistant composition in the extract, as alkaloid, chlorogenic acid etc.
2, the trace copper that has different targets in plant materials and the burdock oligosaccharide that adds among the present invention is combined into burdock oligosaccharide-copper compound.Compare with oligosaccharides with single trace element or list, the induction of resistance of this material strengthens.
3, contain two class materials of sterilization and induction of resistance effect in this invention, compare with the product of present simple function composition, the anti-spectrum of this product is wide, and resistance against diseases strengthens, effect stability.
4, preparation method of the present invention uses plant extract equipment and the fertile production unit assembling of liquid, is convenient to plant extraction liquid and directly pumps into reactor.
5, the present invention's nutritional characteristic and edaphic condition required according to crop is equipped with Different Nutrient Elements targetedly, develops to integrate nutrition, disease-resistant environmentally friendly plant source disease-resistant growth-promoting agent.The anti-spectrum of this disease-resistant growth-promoting agent is wide, by improving crop autoimmunity, promotes robust plant, strengthen the disease resistance of plant, reduce applications of pesticide amount, and then reach the purpose that improves crop yield and quality, and stable performance, nontoxic, harmless to crop, human health and environment are not had danger.
Description of drawings
The environmentally friendly plant source disease-resistant growth-promoting agent of Fig. 1 technological process of production figure.
Fig. 2 embodiment 1 environmentally friendly plant source disease-resistant growth-promoting agent is to the influence of field wheat yield.
Fig. 3 embodiment 2 environmentally friendly plant source disease-resistant growth-promoting agents are to the influence of tomato in plastic greenhouse output.
Embodiment
The invention will be further described below in conjunction with embodiment, but be not limited thereto.
Among the embodiment, described burdock oligosaccharide-copper complex prepares according to the following steps: burdock oligosaccharide 100g is dissolved in the 50mL distilled water, the water-soluble wiring solution-forming of copper sulfate 30g is joined in the burdock oligosaccharide aqueous solution, transfer pH6, refluxed 1 hour in 50 ℃ of stirred in water bath, concentrate, add acetone 15mL and separate out precipitation, filter, wash with water, drying is used the anhydrous methanol recrystallization, vacuum-drying obtains burdock oligosaccharide-copper complex.
Equipment used is the existing conventional equipment in this area among the embodiment.But raw materials used all market is bought.
Embodiment 1: environmentally friendly plant source disease-resistant growth-promoting agent, and the feed composition weight part is as follows:
34 parts of taraxacum extracting solutions; 40 parts of ginkgo extracts; 2 parts of burdock oligosaccharide-copper complexs; 8 parts in saltpetre; 2 parts in sal epsom; 3 parts in zinc sulfate; 10 parts of boric acid; 1 part in copper sulfate.This prescription is specially adapted to wheat.
The preparation method, step is as follows:
(1) with leaf and/or the dry pulverizing after heat of the stem flooding of taraxacum, 60 ℃ of temperature, lixiviate solid-to-liquid ratio are taraxacum dry powder: water=1:30 weight ratio, and extraction time is 2.5 hours, vat liquor filters, and gets the taraxacum extracting solution;
With the lixiviate of Ginkgo Leaf dry powder hot water, 60 ℃ of temperature, lixiviate solid-to-liquid ratio are Ginkgo Leaf dry powder: water=1:35 weight ratio, and extraction time is 2.5 hours, vat liquor filters, and gets ginkgo biloba succi;
(2) above-mentioned plant extraction liquid is input in the reactor in proportion 20 times of thin ups;
(3) add sal epsom, zinc sulfate, boric acid, copper sulfate in proportion in reactor, stirring and dissolving adds 2.4 parts of sequestrant citric acids again, heats 65 ℃, adjust pH to 5.5, carries out chelatropic reaction, reacts 1 hour;
(4) after chelatropic reaction finishes, be cooled to 25～30 ℃, in reactor, add burdock oligosaccharide-copper complex and saltpetre, dissolving, stirring and evenly mixing;
(5) mixing solutions is filtered by vacuum filter, go into the liquid sand mill then and further stir, make mixed solution even.
Further, mixing solutions is input to the finished product still, add tensio-active agent OP-10(account for the feed composition total mass 0.5%), mix, canned, namely get the friendly type plant source disease-resistant of wheat private environment growth-promoting agent product.
Embodiment 2: environmentally friendly plant source disease-resistant growth-promoting agent, and the feed composition weight part is as follows:
30 parts of taraxacum extracting solutions; 35 parts of ginkgo extracts; 2 parts of burdock oligosaccharide-copper complexs; 10 parts of potassium primary phosphates; 2 parts in sal epsom; 3 parts of manganous sulfates; 5 parts in zinc sulfate; 3 parts in ferrous sulfate; 10 parts of boric acid; 1 part in copper sulfate.This prescription is specially adapted to tomato.
The preparation method is with embodiment 1.
Embodiment 3: environmentally friendly plant source disease-resistant growth-promoting agent, and the feed composition weight part is as follows:
37 parts of taraxacum extracting solutions; 25 parts of ginkgo extracts; 2 parts of burdock oligosaccharide-copper complexs; 8 parts of potassium primary phosphates; 2 parts in sal epsom; 5 parts in zinc sulfate; 10 parts of boric acid; 1 part in copper sulfate.This prescription is specially adapted to corn.The preparation method is with embodiment 1.
Embodiment 4: environmentally friendly plant source disease-resistant growth-promoting agent, and the feed composition weight part is as follows:
35 parts of Herba Taraxaci extracts; 30 parts of Folium Ginkgo extracts; 2 parts of burdock oligosaccharide-copper complexs; 10 parts in saltpetre; 2 parts in sal epsom; 5 parts in zinc sulfate; 5 parts in ferrous sulfate; 10 parts of boric acid; 1 part in copper sulfate.This prescription is specially adapted to
Cucumber.The preparation method is with embodiment 1.
The effect experiment
1, among the embodiment 1 the friendly type plant source disease-resistant of wheat private environment growth-promoting agent to the influence of wheat yield and Powdery Mildew
1.1 material and method
1.1.1 test materials: wheat breed: Jinan 15(Shandong Province academy of agricultural sciences crop provides); Bacterial classification: cereal powdery mildew (Erysiphe graminis).Academy of agricultural sciences, Shandong Province plant protection institute provides.
1.1.2 pot experiment:
The pot experiment design: 4 processing are established in this test: (1) contrast (CK): the spray clear water; (2) environmentally friendly plant source disease-resistant growth-promoting agent (BADRGP): spray 10gL
-1Environmentally friendly plant source disease-resistant growth-promoting agent solution; (3) pathogenic bacteria (E.g.): inoculation cereal powdery mildew; (4) BADRGP+E.g.: spray 10gL
-1Environmentally friendly plant source disease-resistant growth-promoting agent solution, inoculation cereal powdery mildew after 3 days.Each handles 6 repetitions, every basin 10 strains.
The pot experiment method: wheat seed is planted in sterilization soil after 3% clorox sterilization, rinsing, vernalization.Treat that wheat grew to for 2 leaf phases, go up spray 10gL toward handling (2) and (4)
-1Environmentally friendly plant source disease-resistant growth-promoting agent solution was inoculated the cereal powdery mildew of having bred to handling (3) and (4) with shaking off method after 3 days.Then wheat is placed that humidity is 90%, temperature is in 25 ℃ the bucket of preserving moisture, to take out behind the 16h.Isolate mutually between each is handled, prevent cross infection, when pending (3) fell ill fully, the investigation disease referred to.
Wheat powdery mildew state of an illness investigation: 0 grade: anosis; 1 grade: lesion area accounts for below 5% of one-piece blade area; 3 grades: lesion area accounts for 6～15% of one-piece blade area; 5 grades: lesion area accounts for 16～25% of one-piece blade area; 7 grades: lesion area accounts for 26～50% of one-piece blade area; 9 grades: lesion area accounts for more than 50% of one-piece blade area.
Disease index (%)=(Σ (state of an illness progression * this grade diseased plant number) * 100)/(the highest number of the state of an illness * total strain number)
Relative control effect (%)=(contrast disease index-processing disease index) * 100/ contrast disease index
1.1.3 field test:
The field experiment design: 3 processing are established in test: (1) contrast (CK): the spray clear water; (2) environmentally friendly plant source disease-resistant growth-promoting agent (BADRGP): spray environmentally friendly plant source disease-resistant growth-promoting agent solution; (3) commercially available foliage fertilizer (SSLF): the product sky of buying on the spray market with disease-resistant function reaches 2116, and 3 repetitions are established in each processing, and district's group arrangement is at random adopted in the residential quarter.
The field experiment method: test is carried out in high-ranking official village, Longshan, Zhangqiu City town.Dilution was 500 times when environmentally friendly plant source disease-resistant growth-promoting agent was used, and the sky reaches 600 times of 2116 dilutions.Spray.Get 4～6m during results
2Wheat, the threshing meter produces, according to actual cut area conversion wheat hectare output.
1.2 result and analysis
1.2.1 environmentally friendly plant source disease-resistant growth-promoting agent is induced the influence to potted plant wheat powdery mildew disease index
Wheat disease index and relative control effect result find out from table 1: do not inoculate the processing of pathogenic bacteria not by infection process.Environmentally friendly plant source disease-resistant growth-promoting agent is induced the disease index of wheat after 3 days significantly to be lower than E.g. and is handled, and relative control effect reaches 33.3%.Illustrate that environmentally friendly plant source disease-resistant growth-promoting agent induces wheat powdery mildew is had certain prophylactic effect.
The environmentally friendly plant source disease-resistant growth-promoting agent of table 1 is to the influence of potted plant wheat powdery mildew disease index
1.2.2 environmentally friendly plant source disease-resistant growth-promoting agent is induced the influence to the field wheat yield
Environmentally friendly plant source disease-resistant growth-promoting agent is induced to the influence of field wheat yield as shown in Figure 2, and the result shows, compares with spraying clear water, spray different foliage fertilizers after, wheat yield has to some extent and improves.The wheat yield that environmentally friendly plant source disease-resistant growth-promoting agent is handled increases by 12.14%, and commercially available foliage fertilizer increases by 9.26%.Illustrate that spraying environmentally friendly plant source disease-resistant growth-promoting agent can increase wheat yield.
If the commercialization production smoothly of environmentally friendly plant source disease-resistant growth-promoting agent, cost can be than experiment, than the further reduction of pilot scale.Price and commercially available foliage fertilizer are similar.Because it also increases production about 2.8% than commercially available foliage fertilizer, if by 1000 jin of per mu yields, the wheat price is 1.2 yuan of/jin calculating, amounts to mu and overcharges about 34 yuan.
1.3 brief summary
The result shows: environmentally friendly plant source disease-resistant growth-promoting agent is induced and can be promoted wheat growth, reduces disease, improves wheat yield.
2, the friendly type plant source disease-resistant of embodiment 2 tomato private environment growth-promoting agent is to the restraining effect of graw mold of tomato
2.1 material and method
2.1.1 for the examination material
Tomato: middle vegetables No. four (Xinjiang Ji Feng kind industry limited liability company is on sale); Bacterial classification: Botrytis cinerea (Botrytis cinerea), referring to Dai Fanglan, Chinese fungi aggregation, 852 pages of the 850th ?, scientific publication, 1979.Academy of agricultural sciences, Shandong Province plant protection institute provides.
2.1.2 pot experiment design and method
4 processing are established in this test, are respectively (1) contrast (CK): the spray clear water; (2) environmentally friendly plant source disease-resistant growth-promoting agent (BADRGP)
10: spray 10gL
-1Environmentally friendly plant source disease-resistant growth-promoting agent solution; (3) (BADRGP)
15: spray 15gL
-1Environmentally friendly plant source disease-resistant growth-promoting agent solution; (4) (BADRGP)
20: spray 20gL
-1Environmentally friendly plant source disease-resistant growth-promoting agent solution, each is handled 5 times and repeats.
Tomato seeds is planted in sterilization soil after 3% clorox sterilization, rinsing, vernalization.Treat that tomato grows to 3 leaves during the phase, final singling, every basin 10 strains, 5 leaves spray environmentally friendly plant source disease-resistant growth-promoting agent solution during the phase, inoculate pathogenic bacteria after 3 days, and method is streak method, is 1 * 10 with concentration
7The Botrytis cinerea liquid of CFU/ml spreads upon tomato leaf portion, and all the other handle the spray clear water.Be placed on humidity then and be 90%, temperature is in 20 ℃ the bucket of preserving moisture, to observe incidence, treats that B.c. handles morbidity when abundant, the statistics disease index.
State of an illness investigation: 0 grade: no scab; 1 grade: lesion area accounts for whole leaf area below 5%; 3 grades: lesion area accounts for whole leaf area 6%～10%; 5 grades: lesion area accounts for whole leaf area 11%～20%; 7 grades: lesion area accounts for whole leaf area 21%～40%; 9 grades: lesion area accounts for whole leaf area more than 40%.
Disease index (%)=(Σ (state of an illness progression * this grade diseased plant number) * 100)/(the highest number of the state of an illness * total strain number)
Relative control effect (%)=(contrast disease index-processing disease index) * 100/ contrast disease index
2.1.3 booth test design and method
3 processing are established in test, are respectively (1) contrast (CK): the spray clear water; (2) environmentally friendly plant source disease-resistant growth-promoting agent (BADRGP): spray environmentally friendly plant source disease-resistant growth-promoting agent solution; (3) commercially available foliage fertilizer (SSLF): the foliage fertilizer sky of buying on the spray market with disease-resistant function reaches 2116, and each is handled 3 times and repeats, and the residential quarter is by district's group arrangement at random, residential quarter area 21m
2
Test is carried out in Hou Yang village village, Liu Ge town, Haiyang City, Shandong Province, 3 years ages of booth canopy.Record whole residential quarter tomato yield during each the results.
2.2 result and analysis
2.2.1 environmentally friendly plant source disease-resistant growth-promoting agent is to the influence of potted plant graw mold of tomato disease index
The environmentally friendly plant source disease-resistant growth-promoting agent of table 2 is to the influence of potted plant graw mold of tomato disease index
Found out that by table 2 the tomato disease index that the environmentally friendly plant source disease-resistant growth-promoting agent of spray different concns is handled significantly is lower than the spray clear water to be handled, that effect is best is (BADRGP)
20Handle, relative control effect reaches 53.77%, illustrates that environmentally friendly plant source disease-resistant growth-promoting agent induces graw mold of tomato is had certain prophylactic effect.
2.2.2 environmentally friendly plant source disease-resistant growth-promoting agent is to the influence of tomato in plastic greenhouse output
Environmentally friendly plant source disease-resistant growth-promoting agent to the influence of tomato in plastic greenhouse output as shown in Figure 3, test-results shows, under greenhouse gardening tomato situation, spray environmentally friendly plant source disease-resistant growth-promoting agent amount of increase in production and reach 22.97%, commercially available foliage fertilizer reaches 19.0%.
After this environmentally friendly plant source disease-resistant growth-promoting agent is used, improve than commercially available foliage fertilizer output nearly 4%, if by winter tomato in plastic greenhouse output be 11000 jin/mu, price is 2 yuan of/jin calculating, mu increases income more than 880 yuan, and therefore environmentally friendly plant source disease-resistant growth-promoting agent benefit is better, has a extensive future.
2.3 brief summary
The result shows that environmentally friendly plant source disease-resistant growth-promoting agent is induced the ability that can improve the tomato botrytis resistant, and sickness rate is reduced, and output increases, and benefit improves.
Claims (10)
1. environmentally friendly plant source disease-resistant growth-promoting agent is characterized in that the feed composition weight part is as follows:
30～50 parts of Herba Taraxaci extracts; 20～40 parts of Folium Ginkgo extracts; 1～2 part of burdock oligosaccharide-copper complex; 5～10 parts in potassium primary phosphate or saltpetre; 0～2 part in sal epsom; 0～5 part of manganous sulfate; 2～5 parts in zinc sulfate; 0～10 part in ferrous sulfate; 1～10 part of boric acid; 0～1 part in copper sulfate;
Chlorogenic acid content 〉=2% in the described taraxacum stem and leaf extract, flavonoid content 〉=1.5%; Alkaloid in the described Folium Ginkgo extract 〉=5.0%, flavonoid content 〉=1.0%;
Described Herba Taraxaci extract is that the lixiviate solid-to-liquid ratio is taraxacum dry powder: water=1:25～30 weight ratios with the filtrate of the leaf of taraxacum and/or the dry pulverizing after heat of stem flooding;
Described Semen Ginkgo extrac is the filtrate by the lixiviate of Ginkgo Leaf dry powder hot water, and the lixiviate solid-to-liquid ratio is Ginkgo Leaf dry powder: water=1:35～40 weight ratios.
Described burdock oligosaccharide-copper complex is to be formed by burdock oligosaccharide and copper sulfate complexing.
2. environmentally friendly plant source disease-resistant growth-promoting agent as claimed in claim 1 is characterized in that the feed composition weight part is as follows:
34 parts of taraxacum extracting solutions; 40 parts of ginkgo extracts; 2 parts of burdock oligosaccharide-copper complexs; 8 parts in saltpetre; 2 parts in sal epsom; 3 parts in zinc sulfate; 10 parts of boric acid; 1 part in copper sulfate;
This prescription is the special-purpose disease-resistant growth-promoting agent of wheat.
3. environmentally friendly plant source disease-resistant growth-promoting agent as claimed in claim 1 is characterized in that the feed composition weight part is as follows:
30 parts of taraxacum extracting solutions; 35 parts of ginkgo extracts; 2 parts of burdock oligosaccharide-copper complexs; 10 parts of potassium primary phosphates; 2 parts in sal epsom; 3 parts of manganous sulfates; 5 parts in zinc sulfate; 3 parts in ferrous sulfate; 10 parts of boric acid; 1 part in copper sulfate; This prescription is the special-purpose disease-resistant growth-promoting agent of tomato.
4. environmentally friendly plant source disease-resistant growth-promoting agent as claimed in claim 1 is characterized in that the feed composition weight part is as follows:
37 parts of taraxacum extracting solutions; 25 parts of ginkgo extracts; 2 parts of burdock oligosaccharide-copper complexs; 8 parts of potassium primary phosphates; 2 parts in sal epsom; 5 parts in zinc sulfate; 10 parts of boric acid; 1 part in copper sulfate; This prescription is the special-purpose disease-resistant growth-promoting agent of corn.
5. environmentally friendly plant source disease-resistant growth-promoting agent as claimed in claim 1 is characterized in that the feed composition weight part is as follows:
35 parts of Herba Taraxaci extracts; 30 parts of Folium Ginkgo extracts; 2 parts of burdock oligosaccharide-copper complexs; 10 parts in saltpetre; 2 parts in sal epsom; 5 parts in zinc sulfate; 5 parts in ferrous sulfate; 10 parts of boric acid; 1 part in copper sulfate; This prescription is the special-purpose disease-resistant growth-promoting agent of cucumber.
6. the preparation method of each described environmentally friendly plant source disease-resistant growth-promoting agent of claim 1～5, step is as follows:
(1) preparation of Herba Taraxaci extract, Folium Ginkgo extract
With leaf and/or the dry pulverizing after heat of the stem flooding of taraxacum, 50～70 ℃ of temperature, lixiviate solid-to-liquid ratio are taraxacum dry powder: water=1:25～30 weight ratios, extraction time is 1～3 hour, vat liquor filters, the chlorogenic acid content of gained stoste 〉=2%, flavonoid content 〉=1.5%; It is Herba Taraxaci extract;
With the lixiviate of Ginkgo Leaf dry powder hot water, 50～70 ℃ of temperature, lixiviate solid-to-liquid ratio are Ginkgo Leaf dry powder: water=1:35～40 weight ratios, and extraction time is 1～3 hour, and vat liquor filters, the alkaloid of gained stoste 〉=5.0%, flavonoid content 〉=1.0%; It is Folium Ginkgo extract;
(2) by proportioning, Herba Taraxaci extract, Folium Ginkgo extract that step (1) is made add in the reactor thin up 20-30 times volume;
(3) by proportioning, micro-sal epsom, manganous sulfate, zinc sulfate, ferrous sulfate, boric acid, copper sulfate in adding in the reactor, stirring and dissolving adds sequestrant citric acid or ethylenediamine tetraacetic acid (EDTA) again, be heated to 60～80 ℃, adjust pH to 4.5～6.5, reacted 0.5～1 hour;
(4) after reaction finishes, be cooled to 25～30 ℃, in reactor, add burdock oligosaccharide-copper complex, potassium primary phosphate or saltpetre, dissolving, stirring is evenly mixed, transfers pH to 5.5～6.5; Filter, further fully stir, make mixed solution even; Namely.
7. the preparation method of environmentally friendly plant source disease-resistant growth-promoting agent as claimed in claim 6, it is characterized in that also adding tensio-active agent in the environmentally friendly plant source disease-resistant growth-promoting agent of gained, mix, canned, the consumption of tensio-active agent is 0.1～0.8wt% of feed composition total amount.
8. the preparation method of environmentally friendly plant source disease-resistant growth-promoting agent as claimed in claim 7 is characterized in that described tensio-active agent is OP-10.
9. the preparation method of environmentally friendly plant source disease-resistant growth-promoting agent as claimed in claim 6 is characterized in that the sequestrant citric acid consumption of step (3) is 5～15wt% of middle micro-consumption.
10. the preparation method of environmentally friendly plant source disease-resistant growth-promoting agent as claimed in claim 6 is characterized in that the leaching process of step (1) is finished in plant concentration extraction device; Vacuum filter is adopted in the filtration of step (4), and described further fully being stirred in the liquid sand mill carried out.
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