CN102812971A - Compound traditional Chinese medicine extract for killing plant pathogenic bacteria and insect pests - Google Patents

Compound traditional Chinese medicine extract for killing plant pathogenic bacteria and insect pests Download PDF

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Publication number
CN102812971A
CN102812971A CN2012102797974A CN201210279797A CN102812971A CN 102812971 A CN102812971 A CN 102812971A CN 2012102797974 A CN2012102797974 A CN 2012102797974A CN 201210279797 A CN201210279797 A CN 201210279797A CN 102812971 A CN102812971 A CN 102812971A
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extract
chinese medicine
dregs
traditional chinese
pathogenic bacteria
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CN2012102797974A
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CN102812971B (en
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戴争
张博
赵玖华
王升吉
孙廷林
任青华
尚佑芬
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Institute of Plant Protection Shandong Academy of Agricultural Sciences
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Institute of Plant Protection Shandong Academy of Agricultural Sciences
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Abstract

The invention discloses a compound traditional Chinese medicine extract for killing plant pathogenic bacteria and insect pests. The compound traditional Chinese medicine extract for killing plant pathogenic bacteria and insect pests is extracted from 20 to 30g of ligusticum chuanxiong hort, 15 to 25g of amur corktree bark, 15 to 25g of the root of Chinese pulsatilla, 20 to 30g of lightyellow sophora root and 30 to 40g of dandelion. An extraction method of the compound traditional Chinese medicine extract for killing plant pathogenic bacteria and insect pests comprises the following steps of 1, crushing the raw materials into crude powder, adding distilled water into the crude powder for immersion of the crude powder, carrying out oscillation, boiling, filtering, adding distilled water into dregs, carrying out decoction, filtering, merging filtrate obtained by the two filtration processes, carrying out concentration, adding a cosolvent into a concentrated solution, and carrying out ultrasonic oscillation to obtain water extract, 2, adding ethanol having a concentration of 95% into dregs obtained by the step 1, carrying out magnetic stirring, carrying out oscillation in a constant temperature oscillator for 2.5 to 3.5 hours, standing, and recovering ethanol so that ethanol extract is obtained, and 3, merging the water extract and the ethanol extract, and carrying out reduced-pressure low-temperature concentration by a rotary evaporator to obtain the compound traditional Chinese medicine extract. An experiment proves that the compound traditional Chinese medicine extract has good effects of killing aphids and blight pathogenic bacteria and can be used as a substitute of a chemical pesticide.

Description

A kind of herbal mixture extract of killing plant pest
Technical field
The present invention relates to a kind of herbal mixture extract of killing plant pest.
Background technology
Have well-known many drawbacks in view of chemical pesticide, agricultural production both at home and abroad at present proposes all that research is efficient, low toxicity, low-residual, non-harmful agricultural chemicals, and exploitation natural plants source pesticide is exactly one of best approach, also is the heat subject of current research.Botanical pesticide derives from plant itself, and environment and human body are had higher safety, is the emphasis of following insecticide research.Traditional Chinese medicine itself is exactly a natural plant, and some Chinese medicine both can be used for the human disease treatment, can also effectively kill plant insect simultaneously, has received showing great attention to of Chinese scholars.In order to seek the best plant resource medicine of killing aphids and anti-blight poison, this seminar is through the repeated screening to multiple folk prescription Chinese medicine and several kinds of different herbal mixtures, and contrast and farmland experiment have finally obtained desirable insecticidal effect with the herbal mixture extract.
From natural plant, seek active substance instead of chemical agricultural chemicals,, become the direction of current agricultural research to reduce chemical pesticide to the injury of human body with to the pollution of environment.
Summary of the invention
To above-mentioned prior art, the invention provides a kind of herbal mixture extract of killing plant pest.
The present invention realizes through following technical scheme:
A kind of herbal mixture extract of killing plant pest is prepared by following bulk drug extraction: rhizome of chuanxiong 20~30g, golden cypress 15~25g, the Chinese pulsatilla 15~25g, kuh-seng 20~30g, dandelion 30~40g;
Method for distilling is:
(1) get each bulk drug, be ground into meal, adding distil water 1000~1800ml soaked 40~60 minutes, vibrated, boiled 50~70 minutes; Cross and filter the filtrating and the dregs of a decoction, dregs of a decoction adding distil water 1000~1200ml decocted 35~45 minutes; Cross and filter the filtrating and the dregs of a decoction, merge filtrating twice, concentrate and obtain the concentrate that the crude drug amount is 1g/ml; In concentrate, add the Tween 80 cosolvent of 2% (that is: adding 2g in the 100ml concentrate), ultrasonic vibration gets the water extract;
(2) the above-mentioned dregs of a decoction add 3 times of (weight multiples) 95% (percentage by volume) ethanol, magnetic agitation 3.5~4.5h, and 37 ℃ of vibration 2.5~3.5h leave standstill 20~28h in the constant-temperature shaking appearance, filter, and reclaim ethanol, get alcohol extract;
(3) merge water extract and alcohol extract, decompression low temperature is concentrated into that to contain crude drug be 1.5g/ml on Rotary Evaporators, promptly get the herbal mixture extract, and 4 ℃ of refrigerators preservations are subsequent use.
Herbal mixture extract of the present invention has good kill activity through experiment showed, to aphid, plant withered pathogenic bacteria, and the substitute that can be used as chemical pesticide is used.
Embodiment
Below in conjunction with embodiment the present invention is further described.
A kind of herbal mixture extract of killing plant pest of embodiment 1 preparation
Prescription is: rhizome of chuanxiong 25g, golden cypress 20g, Chinese pulsatilla 20g, kuh-seng 25g, dandelion 35g;
Method for distilling is:
(1) get each bulk drug, be ground into meal, adding distil water 1400ml soaked 50 minutes, vibrated, boiled 60 minutes; Cross and filter the filtrating and the dregs of a decoction, dregs of a decoction adding distil water 1100ml decocted 40 minutes; Cross and filter the filtrating and the dregs of a decoction, merge filtrating twice, concentrate and obtain the concentrate that the crude drug amount is 1g/ml; In concentrate, add the Tween 80 cosolvent of 2% (that is: adding 2g in the 100ml concentrate), ultrasonic vibration gets the water extract;
(2) the above-mentioned dregs of a decoction add 3 times of (weight multiples) 95% (percentage by volume) ethanol, magnetic agitation 4h, and 37 ℃ of vibration 3h leave standstill 24h in the constant-temperature shaking appearance, filter, and reclaim ethanol, get alcohol extract;
(3) merge water extract and alcohol extract, decompression low temperature is concentrated into that to contain crude drug be 1.5g/ml on Rotary Evaporators, promptly get the herbal mixture extract, and 4 ℃ of refrigerators preservations are subsequent use.
A kind of herbal mixture extract of killing plant pest of embodiment 2 preparations
Prescription is: rhizome of chuanxiong 20g, golden cypress 25g, Chinese pulsatilla 15g, kuh-seng 30g, dandelion 30g;
Method for distilling is:
(1) get each bulk drug, be ground into meal, adding distil water 1000ml soaked 60 minutes, vibrated, boiled 50 minutes; Cross and filter the filtrating and the dregs of a decoction, dregs of a decoction adding distil water 1000ml decocted 35 minutes; Cross and filter the filtrating and the dregs of a decoction, merge filtrating twice, concentrate and obtain the concentrate that the crude drug amount is 1g/ml; In concentrate, add the Tween 80 cosolvent of 2% (that is: adding 2g in the 100ml concentrate), ultrasonic vibration gets the water extract;
(2) the above-mentioned dregs of a decoction add 3 times of (weight multiples) 95% (percentage by volume) ethanol, magnetic agitation 3.5h, and 37 ℃ of vibration 2.5h leave standstill 20h in the constant-temperature shaking appearance, filter, and reclaim ethanol, get alcohol extract;
(3) merge water extract and alcohol extract, decompression low temperature is concentrated into that to contain crude drug be 1.5g/ml on Rotary Evaporators, promptly get the herbal mixture extract, and 4 ℃ of refrigerators preservations are subsequent use.
A kind of herbal mixture extract of killing plant pest of embodiment 3 preparations
Prescription is: rhizome of chuanxiong 30g, golden cypress 15g, Chinese pulsatilla 25g, kuh-seng 20g, dandelion 40g;
Method for distilling is:
(1) get each bulk drug, be ground into meal, adding distil water 1800ml soaked 40 minutes, vibrated, boiled 70 minutes; Cross and filter the filtrating and the dregs of a decoction, dregs of a decoction adding distil water 1200ml decocted 45 minutes; Cross and filter the filtrating and the dregs of a decoction, merge filtrating twice, concentrate and obtain the concentrate that the crude drug amount is 1g/ml; In concentrate, add the Tween 80 cosolvent of 2% (that is: adding 2g in the 100ml concentrate), ultrasonic vibration gets the water extract;
(2) the above-mentioned dregs of a decoction add 3 times of (weight multiples) 95% (percentage by volume) ethanol, magnetic agitation 4.5h, and 37 ℃ of vibration 3.5h leave standstill 28h in the constant-temperature shaking appearance, filter, and reclaim ethanol, get alcohol extract;
(3) merge water extract and alcohol extract, decompression low temperature is concentrated into that to contain crude drug be 1.5g/ml on Rotary Evaporators, promptly get the herbal mixture extract, and 4 ℃ of refrigerators preservations are subsequent use.
Test the killing action of 1 herbal mixture extract to aphid
1 material
1.1 reagent: 95% ethanol, the sincere Bioisystech Co., Ltd in Shandong produces.Benzoic acid (analyzing pure), the strong reagent material Co., Ltd in Shandong, Jinan produces.
1.2 Chinese medicine: rhizome of chuanxiong, golden cypress, the Chinese pulsatilla, kuh-seng, dandelion, purchase authentic medicinal herbs company in the Jinan City, Shandong Province.
1.3 supply the examination polypide: select the individual size aptery aphid consistent with form.
1.4 experimental instrument: WG-5 magnetic force heating stirrer, wound earlier scientific and technological Co., Ltd in Beijing produces.RT-II rotational oscillation appearance, Chengdu medical apparatus and instruments factory produces.The ZH-TI ultrasonic vibrator, pharmacy head factory in Chaoyang, Beijing is produced.SD-2 intelligent control shaking table, Beijing Buddhist nun reaches industry Science and Technology Ltd. and produces.Automatic dual distiller, Huangpu, Shanghai Science and Technology Ltd. produces.
2 methods:
2.1 the preparation of herbal mixture extract: the method by embodiment 1 prepares the herbal mixture extract.
2.2 the sample solution preparation: the herbal mixture extract adds sterile water is diluted to 500mg, 250mg, 125mg, 62.5mg, five concentration gradients of 31.3mg/ml by double dilution method sample solution.
2.3 insecticidal activity assay: the fresh kidney beans leaf that will have aphid is adopted down, rejects nonconforming individuality, and each sample treatment supplies 75~100 of examination worms; Immerse the sample solution of variable concentrations, the unnecessary soup of sucking-off behind the 10s adopts distilled water to do contrast (CK); Place and add a cover the culture dish of preserving moisture; (20-25 ℃) observed 24h-48h under room temperature, and a record worm number alive is represented insecticidal effect with lethality.
3. result:
Experimental result shows that the herbal mixture extract has tangible action of contace poison to aphid, and lethality increases with concentration and significantly increases.Even but when low concentration 31.3mg/ml, aphid is still had certain effect of tagging, see table 1 and table 2.
Table 1 herbal mixture extract 24h is to the killing action of aphid
Table 2 herbal mixture extract 48h is to the killing action of aphid
Test of the influence of 2 herbal mixture extracts to the plant withered pathogenic bacteria
1 material:
1.1 reagent: 95% ethanol, benzoic acid, the sincere Bioisystech Co., Ltd in Shandong produces.Beef extract, peptone, sodium chloride, agar, glucose, the strong reagent material Co., Ltd in Shandong, Jinan produces.
1.2 Chinese medicine: rhizome of chuanxiong, golden cypress, the Chinese pulsatilla, kuh-seng, dandelion, purchase authentic medicinal herbs company in the Jinan City, Shandong Province.
1.3 supply the examination bacterial classification: withered germ of water-melon (Fusarium oxysporumf.sp.niveum), cucumber fusarium axysporum (Fusarium oxysporumf.sp.cucumerinum) provides by key lab of Shandong Agricultural University.
1.4 experimental instrument: WG-5 magnetic force heating stirrer, wound earlier scientific and technological Co., Ltd in Beijing produces.The RT-II is revolved shaker, and Chengdu medical apparatus and instruments factory produces.The ZH-TI ultrasonic vibrator, Chaoyang, Beijing science and technology medicine equipment parent company produces.Automatic dual distiller, Huangpu, Shanghai Science and Technology Ltd. produces.The LXD-5 incubator, Nanjing medical apparatus corporation, Ltd produces.The inhibition zone measuring appliance, Shanghai Red Star medical treatment exploitation responsibility Co., Ltd produces.
2 methods:
2.1 the preparation of herbal mixture extract: the method by embodiment 1 prepares the herbal mixture extract.
2.2 the sample solution preparation: the herbal mixture extract adds sterile water is diluted to 0.5g/ml, 0.25g/ml, 0.125g/ml, 0.0625g/ml, five concentration gradients of 0.0313g/ml by double dilution method sample solution.
2.3 supply the preparation of examination bacterium liquid: get the former bacterium slant activation of former bacterium of watermelon blight and cucumber fusarium axysporum, put 35 ℃ of cultivation 16h in the incubator.Get the good inclined-plane of activation, add the sterile water concussion and shake up, being mixed with concentration is 10 7The cfu/ml bacteria suspension was seeded to plating medium and carries out bacteriostatic experiment in 20 minutes.
2.4 antibacterial activity is measured: cut-off directly is the filter paper dick of 6mm, 100 ℃ of hot air sterilization 2h, and the sterilization filter paper is put into soup and is soaked 30min, and taking-up is dried.With the bacteria suspension for preparing, inject the plate of solid culture medium, evenly coat media surface with aseptic spreader, the aseptic nipper gripping pastille scraps of paper; Put into the bacteria suspension medium, applying light makes the pastille scraps of paper be close to medium; Put 30 ℃ of cultivation 48h in the constant incubator, each cultivation vessel is put 3 of medicinal charts, and each scraps of paper spacing equates; Repeat 3 times, average, measure the antibacterial circle diameter of drug paper disk at last with the inhibition zone measuring appliance.
3. result
Experimental result shows (table 3), and the herbal mixture extract has the obvious suppression effect to former bacterium of watermelon blight and the former bacterium of cucumber fusarium axysporum, and its inhibitory action increases with concentration and significantly increases.Even when low concentration 0.0313g/ml, these two kinds of pathogens are still had the better inhibited effect.
Table 3 herbal mixture extract is to the influence of withered pathogenic bacteria

Claims (1)

1. a herbal mixture extract of killing plant pest is characterized in that: prepared by following bulk drug extraction: rhizome of chuanxiong 20~30g, golden cypress 15~25g, the Chinese pulsatilla 15~25g, kuh-seng 20~30g, dandelion 30~40g; Method for distilling is:
(1) get each bulk drug, be ground into meal, adding distil water 1000~1800ml soaked 40~60 minutes, vibrated, boiled 50~70 minutes; Cross and filter the filtrating and the dregs of a decoction, dregs of a decoction adding distil water 1000~1200ml decocted 35~45 minutes; Cross and filter the filtrating and the dregs of a decoction, merge filtrating twice, concentrate and obtain the concentrate that the crude drug amount is 1g/ml; In concentrate, add 2% Tween 80 cosolvent, ultrasonic vibration gets the water extract;
(2) the above-mentioned dregs of a decoction add 3 times of 95% ethanol, magnetic agitation 3.5~4.5h, and 37 ℃ of vibration 2.5~3.5h leave standstill 20~28h in the constant-temperature shaking appearance, filter, and reclaim ethanol, get alcohol extract;
(3) merge water extract and alcohol extract, decompression low temperature is concentrated into that to contain crude drug be 1.5g/ml on Rotary Evaporators, promptly gets the herbal mixture extract.
CN201210279797.4A 2012-08-08 2012-08-08 Compound traditional Chinese medicine extract for killing plant pathogenic bacteria and insect pests Expired - Fee Related CN102812971B (en)

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Cited By (8)

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Publication number Priority date Publication date Assignee Title
CN103193547A (en) * 2013-04-28 2013-07-10 黄志勐 Environment-friendly plant source disease-resistant growth promoting agent and preparation method thereof
CN104026174A (en) * 2014-06-26 2014-09-10 太仓市荣德生物技术研究所 Sterilizing agent for crops
CN104938540A (en) * 2015-07-17 2015-09-30 曾建民 Preparation method for mosquito and other insect repellent
CN105494482A (en) * 2016-02-03 2016-04-20 孙欣 Natural repellent for forestry pine moth
CN105961459A (en) * 2016-06-13 2016-09-28 吉林长白绿叶人参产业有限公司 Composition for disease and pest control and preparation, preparation method and application thereof
CN106490017A (en) * 2016-10-23 2017-03-15 张颂忠 A kind of foliage fertilizer plant pesticide of preventing and treating tetranychus viennensis
CN106561735A (en) * 2016-08-02 2017-04-19 章吉浦 Plant growth protection liquid and preparation method thereof
CN111820247A (en) * 2020-08-14 2020-10-27 安徽朗宜百草生物工程有限公司 A Chinese medicinal preparation for resisting plant diseases and insect pests, and its preparation method

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CN1466879A (en) * 2003-05-20 2004-01-14 李子友 Pesticide made from traditional Chinese medicine
CN1736207A (en) * 2005-09-07 2006-02-22 刘继东 Chinese medicinal plant sterilizing formulation
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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103193547A (en) * 2013-04-28 2013-07-10 黄志勐 Environment-friendly plant source disease-resistant growth promoting agent and preparation method thereof
CN103193547B (en) * 2013-04-28 2015-02-11 黄志勐 Environment-friendly plant source disease-resistant growth promoting agent and preparation method thereof
CN104026174A (en) * 2014-06-26 2014-09-10 太仓市荣德生物技术研究所 Sterilizing agent for crops
CN104938540A (en) * 2015-07-17 2015-09-30 曾建民 Preparation method for mosquito and other insect repellent
CN105494482A (en) * 2016-02-03 2016-04-20 孙欣 Natural repellent for forestry pine moth
CN105961459A (en) * 2016-06-13 2016-09-28 吉林长白绿叶人参产业有限公司 Composition for disease and pest control and preparation, preparation method and application thereof
CN105961459B (en) * 2016-06-13 2018-12-11 吉林长白绿叶人参产业有限公司 For the composition and its preparation of the prevention and control of plant diseases, pest control, preparation method and application
CN106561735A (en) * 2016-08-02 2017-04-19 章吉浦 Plant growth protection liquid and preparation method thereof
CN106490017A (en) * 2016-10-23 2017-03-15 张颂忠 A kind of foliage fertilizer plant pesticide of preventing and treating tetranychus viennensis
CN111820247A (en) * 2020-08-14 2020-10-27 安徽朗宜百草生物工程有限公司 A Chinese medicinal preparation for resisting plant diseases and insect pests, and its preparation method

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