CN101300984B - Biological preparation with multiple target sites and use thereof - Google Patents
Biological preparation with multiple target sites and use thereof Download PDFInfo
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- CN101300984B CN101300984B CN2008100166641A CN200810016664A CN101300984B CN 101300984 B CN101300984 B CN 101300984B CN 2008100166641 A CN2008100166641 A CN 2008100166641A CN 200810016664 A CN200810016664 A CN 200810016664A CN 101300984 B CN101300984 B CN 101300984B
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Abstract
The present invention discloses a multi-target biological agent and the use thereof. The multi-target biological agent according to the invention contains a complex elicitor and a natural bactericidal substance, wherein the complex elicitor is the composition of shell polysaccharide, acetylsalicylic acid, riboflavin and water-soluble cellulose. The natural bactericidal substance is the composition of sodium benzoate and lemon acid. The pesticide comprises following components, by weight, 30-100 parts of water, 1-8 parts of shell polysaccharide; 0.1-2 parts of acetylsalicylic acid; 0.1-2 parts of riboflavin; 0.1-1 parts of water soluble cellulose; 0.1-2 parts of sodium benzoate; and 0.1-2 parts of lemon acid. The multi-target biological agent provided by the invention has the advantages of stable capability, broad spectrum and low cost.
Description
(1) technical field
The invention belongs to the agricultural chemicals biological technical field, relate to the elicitor compound and the natural disinfection material that can inducing plant produce resistance, be widely used in the prevention of vegetables, crops multiple diseases and the biological preparation with multiple target sites of treatment, the invention still further relates to the application of this biological preparation with multiple target sites.
(2) background technology
Great biological epidemics have become one of most important obstacle of restriction China's agricultural development at present, not only of a great variety, the complicated condition that causes disaster of Agricultural Bio-disasters, and also the loss that causes is serious.China crops due to illness insect pest to cause cereal crops to lose about 35%, vegetables 30%, cotton 33.8%.Because the variability of pest itself, great damage by disease and insect take place frequently, secondary disease insect pest quantity rises, new damage by disease and insect constantly occurs, and has seriously restricted the sustainable development of China's agricultural production and national economy.
Aborning, the control of diseases and pests of agronomic crop at present mainly relies on a large amount of use chemical pesticides, and it is the main cause that causes the harmful substance residual contamination.According to investigations, China suffers the crop acreage of pesticide pollution to reach 1.4 hundred million mu.In addition, because the pollution of agricultural environment, the biocoenosis that comprises various pest natural enemies in the agricultural ecosystem also is subjected to influence in various degree.The prolonged and repeated use of chemical pesticide or abuse have produced other many ecological economy problems simultaneously, as: 1) excessive use chemical pesticide damages crop, reduced quality of agricultural product, this is one of main reason of present agricultural products in China experience green barrier; 2) in the process of the single pesticide species of chronic administration, cause disease, the drug-fast generation of worm inevitably, and in order to remedy the influence of damage by disease and insect pesticide resistance to the chemical pesticide drug effect, have to increase the medication number of times again or improve drug concentration, thereby cause the damage by disease and insect pesticide resistance further to improve, the further increasing of chemical pesticide usage amount, and control efficiency reduces year by year, economic loss is serious; 3) the unreasonable use infringement human health of chemical pesticide, mainly through two approach: the one, in the process of spraying pesticide, owing to applications of pesticide person not the measure of properly protecting pass through tissues such as respiratory tract, skin, digestive tract and directly enter in the human body, cause direct harm; Residue of pesticide in agricultural product, soil, the water body enter in the human body indirectly by diet or food chain on the other hand, just injure health after running up to a certain degree; 4) destroy the ecological balance, when chemicals killed off the insect pests, more large-area its natural enemy that killed was destroyed food chain, and this also is the reason that causes damage by disease and insect to take place in a wider context.
Therefore, along with international development of Agricultural Products Trade and national raising to the food security level of understanding, strengthen non-environmental pollution control, to reduce chemical pesticide to the pollution of environment with realize that agricultural sustainable development is a target, at the production demand, carry out research and product development based on the process for comprehensively treating of biological control, alleviate plant pest, this has also just expedited the emergence of the appearance of biopesticide.Yet existing biopesticide is because biogenetic derivation is single, cause disease and insect resistance general narrow, and drug effect reaction is the inherent shortcoming of biopesticide slowly, unbearable important task when especially running into sudden and crushing damage by disease and insect, and hold at high price, and induction of immunity is difficult to make the plant semelincident immunization.
(3) summary of the invention
It is slow to the present invention is directed to general narrow, the drug effect reaction of present biopesticide disease and insect resistance, and strategy is unilateral, and effect is limited, and a kind of stable performance, broad spectrum activity, biological preparation with multiple target sites that cost is low are provided.The present invention also provides the application of this biological preparation with multiple target sites in controlling plant diseases.
The present invention is by the following technical solutions:
Biological preparation with multiple target sites of the present invention, contain co-induction and natural disinfection material, wherein co-induction is the composition of chitin, acetylsalicylic acid, vitamin b3, water-soluble cellulose, the natural disinfection material is the composition of Sodium Benzoate and citric acid, and the parts by weight of this each component of agricultural chemicals are:
Water 30-100
Chitin 1-8
Acetylsalicylic acid 0.1-2
Vitamin b3 0.1-2
Water-soluble cellulose 0.1-1
Sodium Benzoate 0.1-2
Citric acid 0.1-2.
Above-mentioned biological preparation with multiple target sites, the parts by weight that described component produces better effects are:
Water 50-90
Chitin 2-6
Acetylsalicylic acid 0.5-1.5
Vitamin b3 0.5-1.5
Water-soluble cellulose 0.2-0.8
Sodium Benzoate 0.5-1.6
Citric acid 0.2-1.6.
Above-mentioned biological preparation with multiple target sites, the parts by weight that described component produces optimum efficiency are:
Water 60-80
Chitin 4-6
Acetylsalicylic acid 0.6-1.2
Vitamin b3 0.8-1.2
Water-soluble cellulose 0.2-0.6
Sodium Benzoate 0.6-1.5
Citric acid 0.6-1.5.
The invention still further relates to the application of above-mentioned biological preparation with multiple target sites on the fusarium wilt of preventing and treating vegetables, melon and fruit and crops, downy mildew, powdery mildew, gray mold, early blight, late blight, leaf mold.
Biological preparation with multiple target sites of the present invention mainly contains two action pathway, first elicitor inducing plant resistance expression of gene, and it two is that the natural disinfection material suppresses growth of pathogenic bacteria.Control strategy of the present invention excites plant specificity defense response based on plant resistance to environment stress by exogenous elicitor, thereby improves the resistance of plant to pathogen, and by natural disinfection material kill pathogenic bacteria and its growth of inhibition.The disease-resistant elicitor that the present invention uses is a chitin, acetylsalicylic acid, vitamin b3, water-soluble cellulose and composition thereof, composition-A; The natural antimicrobial substance that the present invention uses is a Sodium Benzoate, citric acid and composition thereof, composition-B; The present invention is with A, and B is mixed with the aqueous solution according to a certain percentage, promptly gets biological preparation with multiple target sites, the aqueous solution has safety, economical, directly, advantage such as practicality.The present invention has good preventive and therapeutic effect to tomato, cucumber, cotton, eggplant, sweet potato, soybean crops, effective to gray mold, anthraenose, fusarium wilt, downy mildew, powdery mildew, early blight, late blight, leaf mold biological physiology disease, can control the generation of above-mentioned illness, its effect is better than conventional pesticide.According in the experimental control of Shandong Pingdu to the tomato in plastic greenhouse gray mold, it drops into 40 yuan of control efficiency that can obtain 150 yuan of conventional pesticides, and the ecological problem that does not have the conventional pesticide medicament residue to be brought, and benefit is very obvious.
(4) embodiment
Embodiment 1
This embodiment biological preparation with multiple target sites is that the raw material by following (part) by weight ratio is mixed into homogeneous phase solution:
Water 70, chitin 5, acetylsalicylic acid 0.7, vitamin b3 1.0, water-soluble cellulose 0.5, Sodium Benzoate 0.7, citric acid 1.0.
Embodiment 2
This embodiment biological preparation with multiple target sites is that the raw material mixed preparing by following (part) by weight ratio forms:
Water 55, chitin 3, acetylsalicylic acid 0.5, vitamin b3 0.6, water-soluble cellulose 0.2, Sodium Benzoate 0.6, citric acid 0.4.
Embodiment 3
This embodiment biological preparation with multiple target sites is formulated by the raw material of following (part) by weight ratio:
Water 40, chitin 1, acetylsalicylic acid 0.2, vitamin b3 0.3, water-soluble cellulose 0.1, Sodium Benzoate 0.3, citric acid 0.2.
Embodiment 4
This embodiment biological preparation with multiple target sites is formulated by the raw material of following (part) by weight ratio:
Water 90, chitin 7, acetylsalicylic acid 1.6, vitamin b3 1.5, water-soluble cellulose 0.8, Sodium Benzoate 1.8, citric acid 1.8.
Product of the present invention is applicable to the disease control of various vegetables, melon and fruit and crops, and is remarkable to the control efficiency of diseases such as fusarium wilt, downy mildew, powdery mildew, gray mold, early blight, late blight, leaf mold, promotes the development of nuisanceless green agricultural product.
Concrete using method: irritate root once earlier, sprayed once every 10-15 days afterwards, irritate root and spray that to be used alternatingly effect preferable.
Experimental example 1 graw mold of tomato efficiency test:
Result of the test is as shown in table 1.200 times, 500 times, 800 times this patent invention products spray to be handled back and clear water (ck) to compare control efficiency remarkable, and secondly 200 times of liquid control efficiency the bests wherein are 500 times.
Table 1 product of the present invention is to the control efficiency (Shandong Pingdu) of tomato in plastic greenhouse gray mold
Experimental example 2 powdery mildew of cucumber efficiency tests
Result of the test is as shown in table 2.200 times, 500 times liquid of this patent invention product spray to be handled the back that the control efficiency of cucumber downy mildew and clear water (ck) are compared otherness is remarkable, and effect all is better than the conventional pesticide Sandofan, and wherein 200 times of liquid effects are more remarkable.
Table 2 product of the present invention is to the control efficiency (Shandong is China ink both) of protection purpleflower violet herb downy mildew
Experimental example 3 cucumber anthracnose efficiency tests
Result of the test is as shown in table 3.200 times of liquid of patented product of the present invention are best to the control efficiency of cucumber anthracnose, secondly are 500 times, and its control efficiency all is better than 600 times of liquid of 75% tpn and significant difference is arranged.
Table 3 product of the present invention is to the control efficiency (Cangshan, Shandong) of plastic tent cucumber anthracnose
Experimental example 4 cotton epidemic disease bacterium efficiency tests
Result of the test is as shown in table 4.200 times, 500 times liquid of patented product of the present invention are better to the control efficiency of cotton epidemic disease bacterium, all are higher than spirit of 85% epidemic disease frost and 70% mancozeb, significant difference.
Table 4 product of the present invention is to the control efficiency (Shou County, Anhui) of experimental field cotton epidemic disease bacterium
Embodiment 5:
This embodiment biological preparation with multiple target sites is that the raw material mixed preparing by following (part) by weight ratio forms:
Water 100, chitin 8, acetylsalicylic acid 2, Riboflavin Tetrabutyrate, water-soluble cellulose 1, Sodium Benzoate 1.5, citric acid 1.9.
Embodiment 6:
This embodiment biological preparation with multiple target sites is that the raw material mixed preparing by following (part) by weight ratio forms:
Water 50, chitin 2.1, acetylsalicylic acid 1.3, vitamin b3 0.6, water-soluble cellulose 1, Sodium Benzoate 1.6, citric acid 1.5.
Embodiment 7:
This embodiment biological preparation with multiple target sites is that the raw material mixed preparing by following (part) by weight ratio forms:
Water 65, chitin 5.5, acetylsalicylic acid 0.7, vitamin b3 1.1, water-soluble cellulose 0.9, Sodium Benzoate 0.5, citric acid 1.3.
Embodiment 8:
This embodiment biological preparation with multiple target sites is that the raw material mixed preparing by following (part) by weight ratio forms:
Water 80, chitin 6, acetylsalicylic acid 0.9, vitamin b3 0.8, water-soluble cellulose 1.2, Sodium Benzoate 0.5, citric acid 1.2.
Claims (4)
1. biological preparation with multiple target sites, it is characterized in that: contain co-induction and natural disinfection material, wherein co-induction is the composition of chitin, acetylsalicylic acid, vitamin b3, water-soluble cellulose, the natural disinfection material is the composition of Sodium Benzoate and citric acid, and the parts by weight of this each component of agricultural chemicals are:
Water 30-100
Chitin 1-8
Acetylsalicylic acid 0.1-2
Vitamin b3 0.1-2
Water-soluble cellulose 0.1-1
Sodium Benzoate 0.1-2
Citric acid 0.1-2.
2. biological preparation with multiple target sites according to claim 1 is characterized in that: the parts by weight of described component are:
Water 50-90
Chitin 2-6
Acetylsalicylic acid 0.5-1.5
Vitamin b3 0.5-1.5
Water-soluble cellulose 0.2-0.8
Sodium Benzoate 0.5-1.6
Citric acid 0.2-1.6.
3. biological preparation with multiple target sites according to claim 2 is characterized in that: the parts by weight of described component are:
Water 60-80
Chitin 4-6
Acetylsalicylic acid 0.6-1.2
Vitamin b3 0.8-1.2
Water-soluble cellulose 0.2-0.6
Sodium Benzoate 0.6-1.5
Citric acid 0.6-1.5.
4. the application of the described biological preparation with multiple target sites of one of claim 1 to 3 on the fusarium wilt of preventing and treating vegetables, melon and fruit and crops, downy mildew, powdery mildew, gray mold, early blight, late blight, leaf mold.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100166641A CN101300984B (en) | 2008-06-04 | 2008-06-04 | Biological preparation with multiple target sites and use thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100166641A CN101300984B (en) | 2008-06-04 | 2008-06-04 | Biological preparation with multiple target sites and use thereof |
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| Publication Number | Publication Date |
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| CN101300984A CN101300984A (en) | 2008-11-12 |
| CN101300984B true CN101300984B (en) | 2010-07-07 |
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| CN2008100166641A Expired - Fee Related CN101300984B (en) | 2008-06-04 | 2008-06-04 | Biological preparation with multiple target sites and use thereof |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103202318B (en) * | 2013-04-08 | 2015-01-28 | 南京师范大学 | Accumulation promoter of atractylis lancea naphtha and preparation method thereof |
| CN104381303B (en) * | 2014-10-28 | 2017-07-21 | 中国农业科学院烟草研究所 | Composition and its application |
| CN107347920A (en) * | 2017-08-22 | 2017-11-17 | 苏州神良生物科技有限公司 | A kind of biological agent for preventing and treating pest and disease damage |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1377579A (en) * | 2002-03-16 | 2002-11-06 | 湖北移栽灵农业科技股份有限公司 | Compound plant resistance revellent |
| WO2005094580A1 (en) * | 2004-03-03 | 2005-10-13 | Isagro S.P.A. | Mixtures and methods for the induction of resistance in plants |
| CN1748511A (en) * | 2005-08-31 | 2006-03-22 | 山东靠山生物科技有限公司 | Biologica land biochemical preparation and its preparing method and use |
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2008
- 2008-06-04 CN CN2008100166641A patent/CN101300984B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1377579A (en) * | 2002-03-16 | 2002-11-06 | 湖北移栽灵农业科技股份有限公司 | Compound plant resistance revellent |
| WO2005094580A1 (en) * | 2004-03-03 | 2005-10-13 | Isagro S.P.A. | Mixtures and methods for the induction of resistance in plants |
| CN1748511A (en) * | 2005-08-31 | 2006-03-22 | 山东靠山生物科技有限公司 | Biologica land biochemical preparation and its preparing method and use |
Non-Patent Citations (1)
| Title |
|---|
| 梁元存等人.激发子诱导植物抗性的作用机制.植物生理学通讯.2001,37(5),442－445. * |
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Granted publication date: 20100707 Termination date: 20180604 |