CN1813543B - Aseptic fly-maggot plant-biological pesticide and its production method - Google Patents
Aseptic fly-maggot plant-biological pesticide and its production method Download PDFInfo
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Abstract
The present invention relates to a kind of aseptic fly maggot plant biological pesticide and its production method. It is made up by using fly maggot as main raw material, adding antibiotic, aspirin, acetaminophen, prednisone acetate and plant extract through a certain preparation process. When it is used, it can be diluted by adding clean water or dissolved, and can be directly sprayed on the diseased plant, and can be used for killing various injurious insects of crops, vegetables and fruit trees.
Description
Technical field
The present invention relates to a kind of non-harmful agricultural chemicals and plant growth accelerator and production method thereof, specifically is that primary raw material prepares agent of insect protected disease-resistant growth-promoting and production method thereof with the fly maggot.
Background technology
The utilization of fly fly maggot has in recent years obtained people's extensive attention, and carried out a large amount of research, past always is considered as the insect of communicate illness by people, mainly be that people are lest catch and kill too late because fly usually is originated in ight soil and corrupt animal, plant, the rubbish.But modern science studies show that fly not only contains good protein and human various necessary amino acid, and contains multiple proteins and lipolytic enzyme, and immunologic active material.The fly maggot fat mass is formed approximate fish oil, and Main Ingredients and Appearance is the unsaturated fatty acid of needed by human.Fly maggot contains micro-abundant in addition, wherein zinc content is high especially, it is one of human development's stage essential trace element, can be used to develop beverage, space food, beauty treatment, sportsman's food etc., also can obtain fat, antibiotic, agglutinin etc. simultaneously in the production process, introduce according to " Xinjiang Animal Husbandry " 1997 the 2nd phases; Can extract chitin from the housing of fly maggot and pupa, be widely used in medicine, chemical industry, printing and dyeing, weaving, feed and biotechnology, maggot powder and other Chinese medicine are mixed with Chinese medicine preparation can play effect hypotensive, fat-reducing.The research that has uses fly maggot oven dry, sterilization, the corresponding Chinese medicine of pulverizing back adding of culturing out to be made into Chinese medicine preparation and to carry out tens routine clinical testings, and the result proves that the hypotensive and fat-reducing effect of fly maggot is remarkable." insect natural enemy " the 2nd phase-59-63 in 2003 has introduced the fly maggot oligo-chitosan strawberry preservation effect has been studied [author] Wei Xin certain herbaceous plants with big flowers thunder towards the inhibitory action of two kinds of fly maggot oligo-chitosans of biology department of bright [mechanism] Guangxi Normal University [digest] to 7 kinds of strawberry pathogens, and result of the test shows that the bacteriostatic activity of enzymolysis oligo-chitosan is significantly higher than H
2O
2The hydrolysis oligo-chitosan, during mass concentration 10g/ml, it has had strong inhibitory effects to 6 kinds of pathogens such as strawberry botrytis cinereas, and fungistatic effect is more than 80%, but general to the inhibitory action of strawberry powdery mildew; H
2O
2The hydrolysis oligo-chitosan also has the good restraining effect to 4 kinds of pathogens such as strawberry botrytis cinereas, and is relatively poor to the inhibitory action of 3 kinds of pathogens such as strawberry black mould germ.Results from pot experiment test shows that fly maggot oligo-chitosan spraying processing can reduce the incidence of disease of strawberry fruit in vegetative period, handle the back and promptly showed certain protection effect on the 3rd day, handle the 6th day protection effect the best in back. handled the back the 9th day, protection effect begins to descend to some extent.The Processing Test of filming is the result show, at normal temperatures, 10-20mg/ml oligo-chitosan solution-treated can make the storage life of strawberry prolong 2-3 days." food industry science and technology " 2002 23 volumes 12 phase .-90-93 have also introduced the development and use and deep processing [author] He Jidong summer Wenshui [mechanism] the Southern Yangtze University Foodstuffs Academy of engineering fly maggot, the fly maggot that [digest] propagates housefly production artificially has very high nutritive value, special bioactive ingredients is arranged again, be worth exploitation energetically.This paper summarizes the developmental research of domestic and international this respect, has proposed the research direction of engineering fly maggot deep development; Utilize fly maggot exploitation new resource food, health food, functional food additives; Chitin extraction, the preparation shitosan is applied to food, medicine, cosmetics; Extract antibacterial peptide, agglutinin to transgenic housefly production biochemical drug.
But except the inventor, open source literature does not in the past appear in the newspapers that fly maggot is applied to plant disease-proof and as plant growth accelerator, is used for antibacterial aspect and does not provide concrete technical scheme yet.
The applicant's Huang is made a show and has been applied for the patent of invention of " a kind of highly effective pesticide and plant growth accelerator and production method thereof with the aseptic fly-maggot preparation " on December 2nd, 2002 to State Intellectual Property Office, [patent application (patent) number] CN02147790.6[summary] a kind of agricultural chemicals and plant growth accelerator and production method thereof, be to be primary raw material with the fly maggot, with its oven dry or break into pulpous state, add antibiotic or preservative, add clear water dilution or dissolving during use, directly be sprayed on the ill plant, the present invention compares with the chemical pesticide of existing control plant, can on various plants, use, also can prevent and treat many diseases of plant, as melon, beans, the powdery mildew of leaf vegetables, leaf rot, yellowtop, the rice blast of paddy rice, banded sclerotial blight, bacterial leaf-blight, the anthracnose of fruit, mycosis, the scab of flowers, yellowtop, leaf rot etc., control efficiency can reach more than 90%, be sprayed directly on the plant leaf surface, can make plant increase output 20-50%.Claims 1, a kind of highly effective pesticide and plant growth accelerator with the aseptic fly-maggot preparation, it is characterized in that: it comprises aseptic fly-maggot, antibiotic or preservative, its composition and mass content are: aseptic fly-maggot (in dry powder), 90-95%; Antibiotic or preservative, 1-10%.The patent of inventor application has been pointed out to use for fly maggot and has been opened up a new road because a large amount of at present use agricultural chemicals, made plant growing more and more difficulty carry out the insect protected diseases prevention, people more and more taste bad non-harmful grain and vegetables.
Summary of the invention
The inventor is on the basis of applying for a patent in 2002, to the deep research of fly maggot application having carried out again, except the material of the special chitin prevention germ of research fly maggot, the chitin that fly maggot is special adds some chemical substances and plant extract material, has found some unexpected effects of fly maggot insect-proof fungicidal and promotion plant growing effect again.
Technical scheme of the present invention is as follows:
Aseptic fly-maggot plant-biological pesticide of the present invention in addition, also adds some chemical substances and plant extract material (Chinese herbal medicine) except comprising aseptic fly-maggot, antibiotic or preservative, and its composition and mass fraction are as follows:
Aseptic fly-maggot 50-80;
Antibiotic or preservative 0.5-2.0;
Aspirin 2.0-10.0;
Paracetamol 0.5-3.0;
Prednisone acetate 2.0-15.0;
Plant extract material 2.0-6.0.
Above-described various material calculates with dry matter.
Above-described aseptic fly-maggot plant-biological pesticide can be made liquid preparation or solid pharmaceutical preparation.
1, the production method of liquid preparation (aqua) aseptic fly-maggot plant-biological pesticide: the aseptic fly-maggot of getting 5-7 days maturations of hatching, it is broken into pulpous state with beater, add antibiotic, chemical substance and plant extract material, perhaps add preservative again, with suitable water wiring solution-forming, the amount that adds entry is 10-80% (weight %).
2, the production method of solid pharmaceutical preparation (pulvis) aseptic fly-maggot plant-biological pesticide: the aseptic fly-maggot of getting 5-7 days maturations of hatching, with baking oven or baker oven dry, break into powder more earlier, add antibiotic, chemical substance and plant extract material, mix, be packaged to be product.
Above-described aseptic fly-maggot plant-biological pesticide, the antibiotic that adds comprises penicillin, jinggangmycin, Polyoxin, terramycin, chloramphenicol etc., and described preservative can adopt food preservative such as materials such as Sodium Benzoate, citric acid, sorbic acid or sodium salt.
Above-described aseptic fly-maggot plant-biological pesticide, the material of plant extract material for from margosa, mother chrysanthemum, kuh-seng, Derris plant, garlic, tobacco, extracting, its extracting method can be according to the traditional Chinese medicine extraction method of routine, and for example water extraction or water are carried and added alcohol extracting method.
Above-described aseptic fly-maggot plant-biological pesticide can add bactericide, insecticide in liquid preparation or solid pharmaceutical preparation, its content is composite as required, generally is no more than 30% and is advisable.
Aseptic fly-maggot plant-biological pesticide with the aseptic fly-maggot preparation can mix with other plant growth regulator, also can add some trace element favourable to plant growing.
The production method of above-described aseptic fly-maggot following (identical) with the inventor's patent application CN02147790.6:
Put it to sterilizing chamber's sterilization 4-8 hour earlier after the fly in field caught, be put into again that shading takes shelter from rain, have in the net of sufficient clean food or the room that glass is made, the nutritious food that allows aseptic fly free choice feeding process sterilize, these foods comprise cornmeal mush, rice congee or peanut cream, necessity can add egg, high nutrients such as milk are laid eggs on ready ovigerous disk, general thickness is 5-10 centimetre, allow its hatching, treat that the fly back of laying eggs shifted out the same day and to be placed to the hatching house in addition in ovigerous disk, lay new ovigerous disk again, 5-7 days, obtain individual stout and strong aseptic fly-maggot, be directly used in the control high-efficiency plant disease medicine of making liquid, also can dry earlier, become agricultural chemicals with other combinations of substances.
Above-described sterilizing chamber can make with glass or net canopy, the underground sterilization tray of laying in the sterilizing chamber, fill the TCCA (Trichloroisocyanuric acid) of potassium permanganate solution or the 0.5-5% of 1-5% in the dish, perhaps 1-5% Eusol, also coat aqueous antiseptic solution around glass or the net canopy, when in sterilization tray or around the sterilizing chamber, stopping after fly enters, after pin, wing, feeler etc. are stained with thimerosal, germ can be killed.
The plant extract material adopts in the above-described aseptic fly-maggot plant-biological pesticide water extraction, water are put forward the method that adds alcohol extracting method and are:
Water extraction:
Get plant (dry product or new plant peel of plucking or fruit, leaf) and thinly slice or pulverize,, put in the container, add 5-8 times of water with water infiltration a few hours (4-6), extraction is boiled in heating, 2-3 hour, takes out soup, the dregs of a decoction add suitable quantity of water again, and heating is boiled extraction, 1-2 hour.Take out soup, extracted twice liquid is merged, heating is condensed into paste and obtains.
Water is carried and is added alcohol extracting method:
Plant (dry product or new plant peel of plucking or fruit, leaf) water is carried secondary, 2 hours first time, 1-1.5 hour for the second time merging extract, filtration, filtrate are concentrated into the clear cream of proportion 1.15, put cold, add ethanol, make the alcohol amount of containing reach 60%-75%, left standstill 48 hours, and filtered filtrate recycling ethanol, the thick paste of dense one-tenth proportion 1.25.
The present invention is that the material of feedstock production can be as the principle of sterilization with the aseptic fly-maggot: fly maggot vitality is extremely strong, evil is given birth to the ground of dirt and can not be caught an illness, reason is can produce the bacteriostasis antibiosis active substance in the body, determine that through scientific research these anti-mattress materials are agglutinin, antibacterial peptide and bacteriolyze enzyme.Antibacterial peptide is a kind of small protein, only needs ten thousand/concentration, just can kill multiple cause of disease mattress, and it is renderd a service and surpasses penicillin, and according to the Japanology personnel, the fly maggot antibacterial peptide can be treated the human diseases that is caused by true mattress infection, not only effectively but also have no side effect.By physics, chemistry and gene engineering method, can induce the rope maggot to produce the high concentration antibacterial peptide.The emphasis investment research of at present more American-European countries, and obtained some progress.
The present invention is that the insect protected mechanism of feedstock production is to have added chemical substance and plant extract materials (Chinese herbal medicine extract) such as some aspirin, prednisone acetate, paracetamol with the aseptic fly-maggot, makes it play synergistic effect.The mechanism of action of these chemical substances and plant extract material below is described:
Aspirin, chemical name: acetylsalicylic acid, chemical formula: C
9H
8O
4Physicochemical property: this product is white crystals or crystalline powder, no stink or littlely have acetic acid smelly, little acid of distinguishing the flavor of, meet moisture and promptly slowly be hydrolyzed into salicylic acid and aceticanhydride, water, the purposes of medicine: antipyretic-antalgic, be used for heating, headache, arthralgic treatment, anti-cardiovascular Aging, but the inventor is through overtesting, find it with other combinations of substances of the present invention after can play and sterilize and prevent the egg hatch effect.Paracetamol (paracetamol) is one of medicine commonly used of analgesic at present, analgesia, treatment flu, this medicine is national basic medical insurance medication, it is again a kind of non-prescribed medicine safely and effectively (0TC), it is evident in efficacy, can be used for the treatment of pain relieving etc. after heating, headache, courbature, arthralgia, neuralgia, dysmenorrhoea, cancer pain, the operation, the inventor is through overtesting, finds can play sterilization and prevent the egg hatch effect after it and other combinations of substances of the present invention.
Prednisone acetate, (the medicine another name: prednisone), chemistry is by name: 17 α, 21-dimonohydric pregnant-1,4-diene-3,11,20-triketone 21-acetate.Molecular formula: C
23H
28O
6Molecular weight: 400.47 have anti-inflammatory, anti-allergic effects, medicine is used for various acute severe bacterial infections clinically, serious anaphylactia, collagenosis, rheumatism, nephrotic syndrome, the serious not enough disease of bronchial astehma, thrombocytopenic purpura, agranulocytosis, acute lymphatic leukemia, various adrenal cortex function, exfoliative dermatitis, neurodermatitis, eczema etc., the inventor is through overtesting, finds can play sterilization and prevent the egg hatch effect after it and other combinations of substances of the present invention.
The plant extract material:
1, the water and the ethanol extract of China tree (Melia azedarach) bark, fruit or leaf, external to worm, particularly ascaris suum has the paralysis effect, the data introduction is arranged, the margosine of variable concentrations increases progressively with concentration the effect of ascaris suum and is slight, moderate to suppressing fully, can reactivate gradually then.To ascaris suum, toosendanin presents paralysis when high concentration, and low concentration has appreciable stimulating action to ascaris suum, occurs acutely shrinking, and can upset the polypide energy metabolism, makes polypide can not adhere to the intestines wall and excretes.Extract with the different solvents of amargosa and fruit carries out bacteriostatic test to two kinds of fungies (aspergillus niger, Trichoderma viride), found that the bacteriostatic activity of the extract of amargosa and fruit variable concentrations has very big difference; The bacteriostatic activity of different solvents extract is also different, see that from integral body the amargosa extract is to better than China tree fruit for the inhibition effect of examination bacterium, the amargosa ethanol extract is the strongest to two kinds of biologically actives for the examination bacterium, Trichoderma viride and aspergillus niger there is good bacteriostatic activity, the test of minimum inhibitory concentration shows: the amargosa ethanol extract is 0.5% to the minimum inhibitory concentration of Trichoderma viride and aspergillus niger, and wherein methanolic extract is 2% to the minimum inhibitory concentration of Trichoderma viride and aspergillus niger.
Mother chrysanthemum, multiple lepidopterous insects there is very strong biologically active, and be a kind of of best results in the current photoactivation toxin, report is arranged, with its maduca sexta of feeding (Manduca sexta) larva, behind UV-irradiation, the larva body weight obviously alleviates, larval phase significant prolongation, then form lopsided pupa, make abnormal pupa rate bring up to 100%, under ultraviolet light, can improve 4333 times the virulence of fruit bat ovum by 10%.Also can be used for the field control larvae.
The Derris plant, can extract separate a kind of from the Derris plant has insecticide active substance, is one of three great tradition vegetable insecticide.At present on producing widely used effective insecticidal constituent rotenone owing to do not have human body endangered and be widely used in using as agricultural chemicals.
Garlic, garlic are the sterilized plant that people are familiar with, and garlic P.E is added, and can reduce the quantity of aphid in garden and the field crops, scale insect, two spotted spider mite.
Tobacco to insect muscle performance toxicity, causes that insect gets food and stop and the paralysis that relaxes.The inventor tests and finds that it shows anesthetic effect to the insect larvae of harm cucumber, there have data to introduce tobacco extract to be very effective as insecticide, can reduce the quantity of aphid, scale insect, white fly, leafhopper, beans fly and flat beetle in garden and the field crops.
The present invention is that the material of feedstock production can be as the principle of plant growth accelerator with the fly maggot, aseptic fly-maggot contains a large amount of protein, trace element and hormone, it can provide the nutrient component of the many needs of plant and the material that promotes plant growing, generally can increase 5-10%.
Biggest advantage of the present invention is, it is a kind of nontoxic, biopesticide efficiently, it can prevent and treat many diseases of plant, as vegetables powdery mildew, leaf rot, the yellowtop of (comprising melon, beans, leaf vegetables), the rice blast of paddy rice, banded sclerotial blight, bacterial leaf-blight, the anthracnose of fruit, mycosis, the scab of flowers, yellowtop, leaf rot etc., control efficiency can reach more than 90%.Also can kill the worm's ovum of a lot of insects, as the worm's ovum of thrips worm, two spotted spider mite, leaf heart worm, rouge and powder worm, acarid, louse worm etc., dominant mechanism is that these worm's ovums can't be hatched, and larva can't grow up to.Efficient reaching more than 98%, but this biopesticide to people and domestic animals and fowls without any toxic and side effect, even sprayed the same day, can not produce harm yet.And present any chemical pesticide does not all reach this requirement.
The present invention can be applied to most of crops, and as melon, beans, vegetables, fruit, flowers, traditional Chinese medicine, forest seedling etc., disease sprays and can tell in 1-2 days, and larva sprays and can tell in 1-2 days, and worm's ovum generally can not be hatched.
The present invention compares with the chemical pesticide (comprising thiophanate methyl, carbendazim, metalaxyl, Pu Like, antimycoin etc.) of existing control plant, can on various plants, use, it can prevent and treat many diseases and the insect pest of plant, and the function singleness of above-mentioned chemical pesticide, what have is only sick responsive to certain, and invalid to other diseases.
Embodiment
Embodiment 1, cultural method by above-mentioned aseptic fly-maggot, put it to sterilizing chamber's sterilization 4-8 hour of the potassium permanganate solution that is mounted with 1-5% after the fly in field caught earlier, be put into again that shading takes shelter from rain, have in the net of sufficient clean food or the room that glass is made, make aseptic fly free choice feeding nutritious by cornmeal mush through what sterilize, rice congee or peanut cream, the food of egg, at the ready corn flour of laying, dregs of beans, wheat bran, feeds such as peanut press pulp, general thickness is to lay eggs on the ovum dish of 5-10 li of product, allow its hatching, 5-7 days, obtain individual stout and strong aseptic fly-maggot, again it is broken into pulpous state, press the per kilogram aseptic fly-maggot and add 25 gram penicillin, 50 gram aspirin; 20 gram paracetamol; 100 gram prednisone acetates; 30 gram margosa extraction of substance (water is carried and added alcohol extracting), again with 500 gram water dilutions, after treating that all substances are all dissolved, filter, install in the clean vial or plastic bottle, obtain product, add the clear water of 500-800 when this product uses, dissolving directly is sprayed on the ill plant, plant prevention for anosis can also be diluted to more than 1000 times and directly spray.
Embodiment 2, cultural method by above-mentioned aseptic fly-maggot, put it to sterilizing chamber's sterilization 4-8 hour that is mounted with 1% the TCCA (Trichloroisocyanuric acid) aqueous solution after the fly in field caught earlier, be put into again that shading takes shelter from rain, have in the net of sufficient clean food or the room that glass is made, make aseptic fly free choice feeding nutritious by cornmeal mush through what sterilize, rice congee or peanut cream, the food of egg, at the ready corn flour of laying, dregs of beans, wheat bran, feeds such as peanut press pulp, general thickness is to lay eggs on the 5-10 li of ovigerous disk, allow its hatching, 5-7 days, obtain individual stout and strong aseptic fly-maggot, again it is broken into pulpous state, press the per kilogram aseptic fly-maggot and add 22 gram Polyoxins, 2 gram Sodium Benzoates, 80 gram aspirin; 10 gram paracetamol; 80 gram prednisone acetates; 50 gram Flos Chrysanthemi Indici extract matter (water extract), again with 500 gram water dilutions, after treating that all substances are all dissolved, filter, install in the clean vial or plastic bottle, obtain product, add the clear water of 500-800 when this product uses, dissolving directly is sprayed on the ill plant, for anosis plant prevention, can also be diluted to 1000 times and directly spray.
Embodiment 3, cultural method by above-mentioned aseptic fly-maggot, put it to sterilizing chamber's sterilization 4-8 hour that is mounted with 8% the bleaching powder aqueous solution after the fly in field caught earlier, be put into again that shading takes shelter from rain, have in the net of sufficient clean food or the room that glass is made, make aseptic fly free choice feeding nutritious by cornmeal mush through what sterilize, rice congee or peanut cream, the food of egg, at the ready corn flour of laying, dregs of beans, wheat bran, feeds such as peanut press pulp, general thickness is to lay eggs on the 5-10 li of ovigerous disk, allow its hatching, 5-7 days, obtain individual stout and strong aseptic fly-maggot, again it is broken into pulpous state, press the per kilogram aseptic fly-maggot and add 15 gram terramycin, 2 gram citric acids, 100 gram aspirin; 8 gram paracetamol; 120 gram prednisone acetates; 30 gram garlic P.E matter (water extract) again with 500 gram water dilutions, are treated all substances all after the dissolving, and filtration installs in the clean vial or plastic bottle, obtains product.Add the clear water of 500-800 when this product uses, dissolving directly is sprayed on the ill plant, for anosis plant prevention, can also be diluted to 1000 times and directly spray.
Embodiment 4, cultural method by above-mentioned aseptic fly-maggot, put it to sterilizing chamber's sterilization 4-8 hour that is mounted with 3% potassium permanganate solution after the fly in field caught earlier, be put into again that shading takes shelter from rain, have in the net of sufficient clean food or the room that glass is made, make aseptic fly free choice feeding nutritious by cornmeal mush through what sterilize, rice congee or peanut cream, the food of egg, at the ready corn flour of laying, dregs of beans, wheat bran, feeds such as peanut press pulp, general thickness is to lay eggs on the 5-10 li of ovigerous disk, allow its hatching, 5-7 days, obtain individual stout and strong aseptic fly-maggot, again it is broken into pulpous state, press the per kilogram aseptic fly-maggot and add 25 gram terramycin, 2 gram sorbic acids, 55 gram aspirin; 25 gram paracetamol; 130 gram prednisone acetates; 25 gram tobacco extract matter again with 500 gram water dilutions, are treated all substances all after the dissolving, and filtration installs in the clean vial or plastic bottle, obtains product.Add the clear water of 500-800 when this product uses, dissolving directly is sprayed on the ill plant, for anosis plant prevention, can also be diluted to 1000 times and directly spray.
Embodiment 5, cultural method by above-mentioned aseptic fly-maggot, put it to sterilizing chamber's sterilization 4-8 hour that is mounted with 4% the chlorine isocyanuric acid aqueous solution after the fly in field caught earlier, be put into again that shading takes shelter from rain, have in the net of sufficient clean food or the room that glass is made, make aseptic fly free choice feeding nutritious by cornmeal mush through what sterilize, rice congee or peanut cream, the food of egg, at the ready corn flour of laying, dregs of beans, wheat bran, feeds such as peanut press pulp, general thickness is to lay eggs on the 5-10 li of ovigerous disk, allow its hatching, 5-7 days, obtain individual stout and strong aseptic fly-maggot, again it is broken into pulpous state, press the per kilogram aseptic fly-maggot and add 25 gram chloramphenicol, 2 gram sorbic acids, 90 gram aspirin; 10 gram paracetamol; 20 gram prednisone acetates; 20 gram margosa extraction of substance (water is carried and added alcohol extracting), 30 gram Flos Chrysanthemi Indici extract matter (water extract) again with 500 gram water dilutions, are treated all substances all after the dissolving, filtration installs in the clean vial or plastic bottle, obtains product.Add the clear water of 500-800 when this product uses, dissolving directly is sprayed on the ill plant, for anosis plant prevention, can also be diluted to 1000 times and directly spray.
Embodiment 6, cultural method by above-mentioned aseptic fly-maggot, put it to sterilizing chamber's sterilization 4-8 hour of the potassium permanganate solution that is mounted with 1-5% after the fly in field caught earlier, be put into again that shading takes shelter from rain, have in the net of sufficient clean food or the room that glass is made, make aseptic fly free choice feeding nutritious by cornmeal mush through what sterilize, rice congee or peanut cream, the food of egg, at the ready corn flour of laying, dregs of beans, wheat bran, feeds such as peanut press pulp, general thickness is to lay eggs on the 5-10 li of ovigerous disk, allow its hatching, 5-7 days, obtain individual stout and strong aseptic fly-maggot, again with its oven dry, press per kilogram aseptic fly-maggot dry powder and add 23 gram penicillin, 40 gram aspirin; 25 gram paracetamol; 70 gram prednisone acetates; 30 gram trifoliate jewelvine extraction of substance (water is carried and added alcohol extracting) mix, and obtain product.Add the clear water of 800-1000 before this product uses, dissolving directly is sprayed on the ill plant.
Embodiment 7, cultural method by above-mentioned aseptic fly-maggot, put it to sterilizing chamber's sterilization 4-8 hour of the potassium permanganate solution that is mounted with 1-5% after the fly in field caught earlier, be put into again that shading takes shelter from rain, have in the net of sufficient clean food or the room that glass is made, make aseptic fly free choice feeding nutritious by cornmeal mush through what sterilize, rice congee or peanut cream, the food of egg, at the ready corn flour of laying, dregs of beans, wheat bran, feeds such as peanut press pulp, general thickness is to lay eggs on the 5-10 li of ovigerous disk, allow its hatching, 5-7 days, obtain individual stout and strong aseptic fly-maggot, again with its oven dry, press per kilogram aseptic fly-maggot dry powder and add 50 gram terramycin, 75 gram aspirin; 45 gram paracetamol; 65 gram prednisone acetates; 35 gram Flos Chrysanthemi Indici extract matter (water extract) mix, and obtain product.Add the clear water of 800-1000 before this product uses, dissolving directly is sprayed on the ill plant.For anosis plant prevention, can also be diluted to 1200 times and directly spray.
Embodiment 8, cultural method by above-mentioned aseptic fly-maggot, put it to sterilizing chamber's sterilization 4-8 hour of the potassium permanganate solution that is mounted with 1-5% after the fly in field caught earlier, be put into again that shading takes shelter from rain, have in the net of sufficient clean food or the room that glass is made, make aseptic fly free choice feeding nutritious by cornmeal mush through what sterilize, rice congee or peanut cream, the food of egg, at the ready corn flour of laying, dregs of beans, wheat bran, feeds such as peanut press pulp, general thickness are to lay eggs on the 5-10 li of ovigerous disk, allow its hatching, 5-7 days, obtain individual stout and strong aseptic fly-maggot,, press per kilogram aseptic fly-maggot dry powder and add 25 gram jinggangmycins again with its oven dry; 80 gram aspirin; 15 gram paracetamol; 30 gram prednisone acetates; 20 gram Flos Chrysanthemi Indici extract matter (water extract), 30 gram shrubby sophora extracts mix, and obtain product.Add the clear water of 800-1000 before this product uses, dissolving directly is sprayed on the ill plant.For anosis plant prevention, can also be diluted to 1200 times and directly spray.
Below be use embodiment of the present invention:
The inventor is applied to the vegetables prevention and elimination of disease and pests with above-mentioned aqua for preparing or pulvis, and the result is as follows:
Application example 1
Worm's ovum with lepidoptera pests such as aseptic fly-maggot plant-biological pesticide control cabbage caterpillar of the present invention, beet armyworms.Cabbage caterpillar, beet armyworm section insect are to cause harm heavier at present and the insect of difficult control, are injured the heaviest with brassicaceous vegetable and shallot.Noctuidae pests happiness temperature and high temperature resistant, high temperature and drought helps big generation.The characteristics that it is caused harm are that the newly hatched larvae blade back of trooping is got food mesophyll, disperse after 3 ages to cause harm laggard people's gluttony phase in 4 ages.Suburb, Guangdong township vegetable field plantation wild cabbage, behind the wild cabbage volume heart, the peasant finds have white butterfly to lay eggs on the dish leaf, the inventor begins to spray medicine in the ovum phase of not incubating, aqua or pulvis aseptic fly-maggot mixture dilute with water 500-800 that the foregoing description is prepared doubly are used for spraying, spray after 5 days 1 time, control efficiency is more than 95% again.Spray and to pluck in back second day, detect through Vegetable Research Institute during harvesting, do not contain harmful agricultural chemicals and poisonous metal element, meet the rank of pollution-free vegetable.
Application example 2
With aseptic fly-maggot plant-biological pesticide control tomato virus disease of the present invention, the inventor doubly is used to spray the tomato virus disease with aqua or the pulvis aseptic fly-maggot mixture dilute with water 500-1000 that the foregoing description prepares, spray back two space-based instincts and eliminate disease, tomato restore normal growth.And compared with the control, volume increase 20%;
Application example 3
The sugarcane of Heng County, Guangxi peasant household plantation suffers from powdery mildew, when 1 meter high of sugarcane, occur in the plant middle part and produce subcircular powdery white mould, after be fused into powder domestic animal spot, be covered with full leaf when serious: plant strain growth is bad, the inventor doubly is used to spray powdery mildew with aqua or the pulvis aseptic fly-maggot mixture dilute with water 800-1000 that the foregoing description prepares, and sprays back two space-based instincts and eliminates disease, and sugarcane restore normal growth.Spray 1 time after spending three days, disease is eliminated again, and sugarcane grows fine.
Application example 4
5 mu in the paddy rice of school, Heng County, Guangxi chair Zhen Huang village Huang plantation in 2005, nearly having, 3 mu paddy rice suffers from rice blast, the inventor doubly is used to spray paddy rice 2 times with aqua or the pulvis aseptic fly-maggot mixture dilute with water 800-1000 that the foregoing description prepares, spray back 3-5 space-based instinct and eliminate disease, paddy rice restore normal growth, and increases output 10% than common rice.
Application example 5
Suburb, Nanning peasant thanks so-and-so and has planted 2 mu of kidney beans, when bean seedlings length to 30 is centimetre high, find that the kidney bean leaf has tetranychid to suck juice at blade back, chlorisis color spot point is appearred by harm, after become white or yellow-white, transfer redness at last to, the blade that the has rubescent shape of seemingly burning that dries up, the inventor doubly is used for aqua or the pulvis aseptic fly-maggot mixture dilute with water 800-1000 that the foregoing description prepares to spray, and sprays back two space-based instincts and eliminates disease, and kidney bean restore normal growth.Spray 1 time after spending three days, disease is eliminated again, and kidney bean grows fine, and the plant strain growth stalwartness.
Application example 6
Suburb, Nanning peasant Lee has planted 4 mu of capsicums, and capsicum meets with the harm of mite class, on capsicum or the pimento, plant leaf shrinkage after the mite class occurs, plant even fern leaf phenomenon occurred when serious, and it is virus disease that the vegetable grower takes for, and presses virus disease control, effect is very poor.Examine analysis through the expert, predicate the harm of mite class, the young tender position of adult or young mite harm plant, the leaf back at position of being injured is taupe or yellowish-brown, has oily gloss or oil immersion shape, and the blade outer rim is curled downwards, though sick leaf shrinkage has tangible scar at the back side of leaf; Virus disease also has symptoms such as leaf roll, deformity, and the inventor doubly is used for aqua or the pulvis aseptic fly-maggot mixture dilute with water 800-1000 that the foregoing description prepares to spray, and sprays back two space-based instincts and eliminates disease, and capsicum restore normal growth.Spray 1 time after spending 5 days, disease is eliminated again.
The aseptic fly-maggot plant-biological pesticide control citrus red mite test of pesticide effectiveness of the present invention
The aseptic fly-maggot plant-biological pesticide aqua, the time: in Guilin city Pingyue County citrus garden carried out the mite danger control citrus red mite test of pesticide effectiveness to June in May, 2005, obtained the better prevention effect, now result of the test is summarized as follows.
1, reagent agent
The aseptic fly-maggot plant-biological pesticide aqua; 5% hexythiazox missible oil (the Zhejiang Weir reaches company and produces).
2, test site
The test of pesticide effectiveness is chosen in citrus garden, Pingyue County, Guilin city carries out, and these tangerine gardens citrus red mite in 2005 is taken place more serious.
3, experimental scheme
4 processing are established in test altogether, 1. aseptic fly-maggot plant-biological pesticide aqua (800 times of dilute with waters), 2. aseptic fly-maggot plant-biological pesticide aqua (1000 times of dilute with waters), 3. aseptic fly-maggot plant-biological pesticide aqua (2000 times of dilute with waters), 4. clear water contrast.Every sub-district 5 strain citrus, 3 repetitions, randomized arrangement.3 peasant households are arranged in demonstration, and each handles citrus garden 120 strains tree, does not establish repetition.
4, investigation method
Investigation medicine frontal lobe acarid mouth radix, the worm mite number of 5d, 10d, 20d behind the medicine.The every strain tree of the test of pesticide effectiveness is by orientation, the four corners of the world, and 5 leaves on 1 branch are investigated in each orientation.Each handles investigation 6 strains tree the demonstration area, and every strain tree is looked into 5 branches at random, and each branch is looked into 5 leaves.Result of the test is calculated by the test of pesticide effectiveness and demonstration test compound average.
5, test results and analysis
Result of the test shows that the preventive effect of mite danger aseptic fly-maggot plant-biological pesticide aqua (800 times of dilute with waters) has reached 85.64% behind dispenser 5d, has shown its positive effect.Aseptic fly-maggot plant-biological pesticide aqua behind the 10d (800 times of dilute with waters) preventive effect has reached 92.64%.
Control efficiency compared with the control:
Behind aseptic fly-maggot plant-biological pesticide aqua (800 times of dilute with waters) the medication 10d, treatment effect reaches 92.64%, with the preventive effect height of contrast 5% hexythiazox 66.77%.The effect comparison that aseptic fly-maggot plant-biological pesticide aqua control citrus red mite is described is far better according to hexythiazox, hexythiazox is an application effect kind preferably in producing, and the effect of aseptic fly-maggot plant-biological pesticide aqua (800 times of dilute with waters) is also better than hexythiazox, so we think that aseptic fly-maggot plant-biological pesticide aqua (800 times of dilute with waters) has higher using value aborning.
6, conclusions and recommendations
Aseptic fly-maggot plant-biological pesticide aqua (800 times of dilute with waters) control citrus red mite effect all can effectively be controlled its ascendant trend before the 20d behind the 5d behind medicine, reach preventive effect preferably, can large-area applications promote.
Claims (5)
1. aseptic fly-maggot plant-biological pesticide, it is characterized in that: its main active comprises following material, its composition and mass fraction are as follows:
Aseptic fly-maggot 50-80;
Antibiotic 0.5-2.0;
Aspirin 2.0-10.0;
Paracetamol 0.5-3.0;
Prednisone acetate 2.0-15.0;
Plant extract material 2.0-6.0;
Above-described various material calculates with dry matter;
Described antibiotic is penicillin, jinggangmycin, Polyoxin, terramycin or chloramphenicol;
Described plant extract material adds the material that alcohol extracting method obtains for adopting water extraction or water to carry from margosa, mother chrysanthemum, kuh-seng, Derris plant, garlic or tobacco.
2. aseptic fly-maggot plant-biological pesticide according to claim 1 is characterized in that: described aseptic fly-maggot plant-biological pesticide is liquid preparation or solid pharmaceutical preparation.
3. aseptic fly-maggot plant-biological pesticide according to claim 1 is characterized in that: described aseptic fly-maggot plant-biological pesticide also further adds preservative, and described preservative is Sodium Benzoate, citric acid, sorbic acid or sodium salt.
4. the production method of an aseptic fly-maggot plant-biological pesticide as claimed in claim 1 or 2, it is characterized in that: the production method of liquid preparation biopesticide is to get the fly maggot of above-mentioned hatching 5-7 days maturation, break into pulpous state with beater, add antibiotic, aspirin, paracetamol, prednisone acetate and plant extract material, with suitable water wiring solution-forming.
5. production method as claim 1 and 2 described aseptic fly-maggot plant-biological pesticides, it is characterized in that: the production method of solid pharmaceutical preparation biopesticide is to get the fly maggot of above-mentioned hatching 5-7 days maturation, earlier with baking oven or baker oven dry, break into powder again, add antibiotic, aspirin, paracetamol, prednisone acetate and plant extract material, mix, be packaged to be product.
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